KR900009979A - Japanese encephalitis virus non-structural protein production method - Google Patents

Japanese encephalitis virus non-structural protein production method Download PDF

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Publication number
KR900009979A
KR900009979A KR1019890018246A KR890018246A KR900009979A KR 900009979 A KR900009979 A KR 900009979A KR 1019890018246 A KR1019890018246 A KR 1019890018246A KR 890018246 A KR890018246 A KR 890018246A KR 900009979 A KR900009979 A KR 900009979A
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Prior art keywords
encephalitis virus
japanese encephalitis
cdna encoding
protein
nonstructural protein
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KR1019890018246A
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Korean (ko)
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고따로 야스이
미찌꼬 미야모또
다까노리 사또
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원본미기재
닛뽕 제온 가부시끼가이샤
자이단호오진도오꾜도신께이가가꾸소오고겡뀨쇼
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Publication of KR900009979A publication Critical patent/KR900009979A/en

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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
    • C12N2770/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
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Abstract

내용 없음No content

Description

일본 뇌염 비루스 비구조 단백질의 제조방법Japanese encephalitis virus non-structural protein production method

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 본 발명의 방법을 나타낸다. 제2도는 일본 뇌염 비루스 cDNA 클로닝의 방법을 나타낸다. 제3도는 cDNA에서 제한 효소 부위의 위치관계를 나타낸다.1 shows the method of the present invention. 2 shows a method of cloning Japanese encephalitis virus cDNA. 3 shows the positional relationship of restriction enzyme sites in cDNA.

Claims (5)

일본 뇌염 비루스 비구조 단백질을 코딩하는 cDNA의 일부분 이상이 비클로비루스 증식에 비필수적인 게놈 영역에 통합되어 있는 제조합 비클로비루스를 사용하여 곤충 세포를 감염시키고, 이 곤충 세포를 배양하고, 발현된 일본 뇌염 비루스 비구조 단백질을 회수함을 특징으로 하는 일본 뇌염 비루스 비구조 단백질의 제조방법.Infected insect cells, cultured, and expressed using presynthetic vicloviruses wherein at least a portion of the cDNA encoding the Japanese encephalitis virus nonstructural protein is integrated into genomic regions that are indispensable for biclovirus growth A method for producing Japanese encephalitis virus non-structural protein, characterized by recovering Japanese encephalitis virus non-structural protein. 제1항에 있어서, 비구조 단백질을 코딩하는 cDNA의 전부 또는 일부가 일본뇌염 비루스 비구조 단백질-1인 방법.The method of claim 1, wherein all or part of the cDNA encoding the nonstructural protein is Japanese Encephalitis Virus Nonstructural Protein-1. 제2항에 있어서, 비구조 단백질-1을 코딩하는 cDNA의 전부 또는 일부가 일본뇌염 비루스 비구조 단백질-2인 방법.The method of claim 2, wherein all or part of the cDNA encoding nonstructural protein-1 is Japanese Encephalitis Virus Nonstructural Protein-2. 제1항 또는 2항에 있어서, 일본뇌염 비루스 비구조 단백질을 코딩하는 cDNA가 제4도에서 NAI으로 나타낸 아미노산 서열 또는 아미노산 서열과 실질적으로 동일한 기능을 갖는 폴립펩티드를 코팅하는 방법.The method according to claim 1 or 2, wherein the cDNA encoding the Japanese encephalitis virus nonstructural protein has a function of substantially the same as the amino acid sequence or amino acid sequence represented by NAI in FIG. 일본 뇌염 비루스 엔벨로프 단백질을 코딩하는 cDNA 또는 3'-말단을 포함하는 그의 일부, 일본뇌염 비루스 비구조 단백질-1을 코딩하는 cDNA, 일본 뇌염 비루스 비구조 단백질-2를 코딩하는 cDNA 또는 그의5'-말단을 포함하는 cDNA, 5'-말단에서부터 3'-말단까지 계속되는 것의 일부를 비클로비루스 증식에 비필수적인 게놈 영역에 통합시킨 재조합 바클로비루스를 사용하여 고층세포를 감염시키고, 이곤충 세포를 배양하고, 발현된 일본 뇌염 비루스 비구조단백질을 회수함을 특징으로 하는 일본 뇌염 비루스 비구조 단백질의 제조방법.CDNA encoding the Japanese encephalitis virus envelope protein or part thereof comprising the 3'-terminus, cDNA encoding the Japanese encephalitis virus nonstructural protein-1, cDNA encoding the Japanese encephalitis virus nonstructural protein-2 or its 5'- Infecting the high-rise cells using recombinant baclovirus, which incorporates a terminal cDNA, part of the 5'-end to the 3'-end, into a genomic region that is indispensable for biclovirus growth, and cultures the insect insect cells And recovering the expressed Japanese encephalitis virus non-structural protein. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019890018246A 1988-12-09 1989-12-09 Japanese encephalitis virus non-structural protein production method KR900009979A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP88-311656 1988-12-09
JP63311656A JPH02156896A (en) 1988-12-09 1988-12-09 Production of non-constitutive protein of japanese encephalitis virus

Publications (1)

Publication Number Publication Date
KR900009979A true KR900009979A (en) 1990-07-06

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ID=18019906

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KR1019890018246A KR900009979A (en) 1988-12-09 1989-12-09 Japanese encephalitis virus non-structural protein production method

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JP (1) JPH02156896A (en)
KR (1) KR900009979A (en)
FR (1) FR2640144A1 (en)
GB (1) GB2226031A (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2511494B2 (en) * 1988-05-12 1996-06-26 善治 松浦 Method for producing Japanese encephalitis virus surface antigen protein
FR2665710A1 (en) * 1990-08-10 1992-02-14 Pasteur Institut RECOMBINANT BACULOVIRUS EXPRESSING E AND NS1 PROTEINS FROM FLAVIVIRIDAE - RELATED VIRUSES OR FLAVIVIRIDAE - RELATED VIRUSES, DIAGNOSTIC AND THERAPEUTIC APPLICATIONS.
WO2004082712A1 (en) * 2003-03-20 2004-09-30 Shanghai Tengen Biomedical Co., Ltd. Bivalent vaccine
US10718771B2 (en) 2018-02-09 2020-07-21 Chung Yuan Christian University Recombinant baculoviruses and their uses in detecting arthropod-borne virus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01501203A (en) * 1986-10-27 1989-04-27 フォーニアー，モーリル・ジェイ Diagnosis and vaccines for Japanese encephalitis virus and similar viruses
JP2511494B2 (en) * 1988-05-12 1996-06-26 善治 松浦 Method for producing Japanese encephalitis virus surface antigen protein

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GB8927844D0 (en) 1990-02-14
JPH02156896A (en) 1990-06-15
FR2640144A1 (en) 1990-06-15
GB2226031A (en) 1990-06-20

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