KR880000580A - 융합 방법 - Google Patents

융합 방법 Download PDF

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Publication number
KR880000580A
KR880000580A KR1019870006298A KR870006298A KR880000580A KR 880000580 A KR880000580 A KR 880000580A KR 1019870006298 A KR1019870006298 A KR 1019870006298A KR 870006298 A KR870006298 A KR 870006298A KR 880000580 A KR880000580 A KR 880000580A
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KR
South Korea
Prior art keywords
fusion
cells
carried out
fusing
chemical
Prior art date
Application number
KR1019870006298A
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English (en)
Inventor
피. 선카라 사이
Original Assignee
개리디. 스트리트
메렐다우 파마슈티칼스 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 개리디. 스트리트, 메렐다우 파마슈티칼스 인코포레이티드 filed Critical 개리디. 스트리트
Publication of KR880000580A publication Critical patent/KR880000580A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

내용 없음

Description

융합 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (11)

  1. 융합될 세포의 혼합물을 유효량의 응집원과 접촉시킨 다음 응집된 세포를 융합시킴을 특징으로 하여, 선택성 조건하에서 유용한 생성물을 생성할 수 있는 포유동물 세포와 연속 증식가능한 포유동물세포를 융합시켜 하이브리드세포를 제조하는 방법.
  2. 유용한 생성물을 생성할 수 있는 식물세포 및 연속증식 가능한 식물세포를 유효량의 응집원과 접촉시킨다음 응집된 세포를 융합시킴을 특징으로하여 융합된 하이브리드 세포를 제조하는 방법.
  3. 제1항에 있어서, 응집원이 식물성 혈구응집소(phytohaemaggiutinin)임을 특징으로 하는 방법.
  4. 제2항에 있어서, 응집원이 식물성 혈구응집소임을 특징으로 하는 방법.
  5. 제1항에 있어서, 융합을 화학적 융합법으로 수행함을 특징으로 하는 방법.
  6. 제2항에 있어서, 융합을 화학적 융합법으로 수행함을 특징으로 하는 방법.
  7. 제1항에 있어서, 융합을 전기-융합법으로 수행함을 특징으로 하는 방법.
  8. 제2항에 있어서, 융합을 전기-융합법으로 수행함을 특징으로 하는 방법.
  9. 제5항에 있어서, 화학적 융합원이 약 1000 내지 약 4000의 평균 분자량을 갖는 PEG임을 특징으로 하는 방법.
  10. 제6항에 있어서, 화학적 융합원이 약 1000 내지 약 4000의 평균 분자량을 갖는 PEG임을 특징으로 하는 방법.
  11. 제1항 또는 2항에 따라 생성되는 융합된 하이브리드세포.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019870006298A 1986-06-24 1987-06-22 융합 방법 KR880000580A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US87809186A 1986-06-24 1986-06-24
US878091 1992-05-04

Publications (1)

Publication Number Publication Date
KR880000580A true KR880000580A (ko) 1988-03-28

Family

ID=25371357

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019870006298A KR880000580A (ko) 1986-06-24 1987-06-22 융합 방법

Country Status (13)

Country Link
EP (1) EP0251106A3 (ko)
JP (1) JPS633792A (ko)
KR (1) KR880000580A (ko)
CN (1) CN87104270A (ko)
AU (1) AU591298B2 (ko)
DK (1) DK319787A (ko)
FI (1) FI872787A (ko)
HU (1) HUT44077A (ko)
IL (1) IL82971A0 (ko)
NO (1) NO872629L (ko)
NZ (1) NZ220765A (ko)
PT (1) PT85150B (ko)
ZA (1) ZA874409B (ko)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8928234B2 (en) 2009-12-14 2015-01-06 Lg Electronics Inc. Lighting apparatus
US9030460B2 (en) 2012-03-05 2015-05-12 Samsung Display Co., Ltd. Display apparatus

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA874410B (en) * 1986-06-24 1988-04-27 Merrell Dow Pharma Fusion products
US5175004A (en) * 1988-12-27 1992-12-29 Matsumura Kenneth N Propagatable, new combinant cells for cellular replacement therapy
CN108707600A (zh) * 2018-05-31 2018-10-26 东北农业大学 一种猪鼠细胞单细胞融合方法
CN108707599A (zh) * 2018-05-31 2018-10-26 东北农业大学 一种小鼠胚胎干细胞单细胞融合方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5722697A (en) * 1980-07-15 1982-02-05 Hiroshi Taniguchi Novel preparation method of bioproducing substance
ZA874410B (en) * 1986-06-24 1988-04-27 Merrell Dow Pharma Fusion products

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8928234B2 (en) 2009-12-14 2015-01-06 Lg Electronics Inc. Lighting apparatus
US9030460B2 (en) 2012-03-05 2015-05-12 Samsung Display Co., Ltd. Display apparatus

Also Published As

Publication number Publication date
FI872787A (fi) 1987-12-25
FI872787A0 (fi) 1987-06-23
PT85150B (pt) 1990-03-30
JPS633792A (ja) 1988-01-08
AU591298B2 (en) 1989-11-30
EP0251106A3 (en) 1989-10-04
NZ220765A (en) 1990-06-26
DK319787D0 (da) 1987-06-23
PT85150A (en) 1987-07-01
IL82971A0 (en) 1987-12-20
ZA874409B (en) 1988-04-27
NO872629D0 (no) 1987-06-23
EP0251106A2 (en) 1988-01-07
AU7451487A (en) 1988-01-07
HUT44077A (en) 1988-01-28
DK319787A (da) 1987-12-25
CN87104270A (zh) 1988-01-27
NO872629L (no) 1987-12-28

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