KR880000002B1 - Pesticidal composition - Google Patents

Abstract

Nitrofuran derivatives (I), having activity for bacterially caused plant disease, were prepared and evaluated germicidal effect of their composition mixture. When nutrition-culture ground, has been cultivated by bacteria, was treated by nitrofuran derivatives, No. CH-819(5-nitro-furfurylyten-3-amino-2-oxazolidon) and CH-1552 (3-(5- nitro-furfurilyten-amino)-imidazole-2-thiol), activity of the pesticidal composition for atroseptica was greater than Erbinia Caroto when concentration was 1.0-1.5 mg/ml, volume was 0.1ml/hole.

Description

Bactericidal composition

Figure 1 shows the susceptibility of plant pathogens and bacteria to each mixture when carbendazim and furazolidone are mixed in various proportions.

The present invention relates to a bactericidal composition having activity against bacterial plant diseases, a method for preparing the same, and a method of using the composition to suppress bacterial plant diseases.

In the above formula, X means a structural formula (a) group or (b) group.

And a bactericidal composition active against bacterial plant diseases comprising 1-99% of the total amount by weight of any one or more known bactericidal active ingredients. On the other hand, when the composition consists of two active ingredients, the ratio of the active ingredient of the general formula (I) to the known bactericidal active ingredient is 1: 99-99: 1, preferably 1: 9: -9: 1 When the composition consists of three active ingredients, it is 1: 9: 0.5-1: 1: 1, preferably 1: 4: 1-1: 1: 1, and these compositions are suitable inert solid or liquid carriers or diluents. And conventional adjuvants such as wetting agents, dispersants, adhesives, antifoams and / or cryoprotectants.

The compositions of the present invention are useful for inhibiting bacterial or bacterial and fungal diseases of plants, respectively:

Agrobacterium Tumefaciens O

Agrobacterium Tumefaciens C-58

Agrobacterium Tumefaciens B-6

Agrobacterium RadioBacter K-84

Erwinia Uredobora

Erwinia Carotoborah var. Atroceptika

Erwinia Carotoborah

Corinbacterium misigananese

Corinthium Nebraskense

Santomona genus

Genus Fusarium, Genus Littoonia, Genus Botrytis, Genus Screrotinian, Genus Alternaara, Genus Penicillium, Genus Aspergillus, Genus Rizopus.

The bactericidal composition contains, in addition to the general formula (I), one or more known bactericidal active ingredients. Suitable known folding and systemic fungicides are used for this purpose. Another purpose of using other fungicides is to extend the active range of the composition. The following sterilizations are preferred for use.

1-Butylcarbamoyl-benzimidazole-2-methyl-carbamade (benomyl):

2-Methoxinecarbonylamino-benzimidazole (MBC):

1, 2-bis-3-methoxycarbonyl-thiourea-benzene (thiophanatemethyl):

2- (4-thiazolyl) -benzimidazole (thiabendazole):

Zinc-Ethylene-Bis-Dithiocarbamate (Manap):

Manganese-Zink-Ethylene-Bis-Dithiocarbamate

Tetramethyl-thiuram-disulfide (TMTD):

N-trichloro-methylthio-tetrahydro-phthalamide (captan).

The compositions of the present invention are particularly suitable for the protection of propagation materials such as seeds, tubers, calibers, cuttings, etc., prior to sowing or during storage. In addition to most fungal infections, plants can be harmed by various plant pathogenic bacteria.

Krement (Klement, Plant Protection, 1982, No. 37.) summarizes the important features of diseases caused by phytopathogenic bacteria:

Of the plant pathogenic bacteria mentioned above, bacteria which infect breeding substances, for example seeds, tubers, rhizomes, and trees, are particularly important.

In other words, there are Corinbacterium microgigancene of tomato and Corinbacterium nebrasucaense of corn. Agrobacterium tumefaciens causes large tumors in various fruit varieties and vine propagation, and infected material becomes unusable. Among plant pathogenic bacteria, Erwinia species cause soft rot on stored crops with high levels of water and juice, especially because of the high economic loss of breeding materials-tubers, rhizomes, onions, roots. Harmful.

Ermine Carotoborah var. Atheroseptica causes soft disease in stored potatoes, and the harm caused by poor protection is very serious.

According to Pterter, Plant Protection "Novenyvedelem", 1982, No. 37, when 5% of all potatoes develop a total of 1 million forints (Hungarian halves) when the disease occurs.

Already discussed the severity of bacteria (Ervinia Carotovora var. Atheroceptica) and fungal diseases. [Note: Stachewich, 1974. Nachr. Blatt Pflschutz., 28. (s)., Wellving, Sweed. Seed. Assoc. 31, Anonym, Merkbl. des Pflschutz, 1974. (10) 7.

As such, fungal and bacterial diseases can develop simultaneously in potatoes during storage and prior to sowing.

Insecticidal active ingredients used in fungal and bacterial diseases at the same time are summarized in Table 1 and mentioned in the prior art.

TABLE 1

However, none of the compositions described above is suitable for full protection of plants and antibiotics should be used to suppress bacterial infection. In most countries, however, the use of antibiotics for agriculture is prohibited.

It is an object of the present invention to overcome the shortcomings of known methods for suppressing bacterial plant diseases. In particular, an object of the present invention is to effectively treat bacterial plant diseases, and to provide a plant protection agent suitable for simultaneously treating bacterial and fungal diseases.

From experiments on livestock, it has been found that nitrofuran derivatives are active against various Gram-negative bacteria. In such prior art, there is no known use of nitrofuran derivatives for plant protection.

In addition, when the nitrofuran derivative is used in combination with other known fungicides, the present invention was found to have a synergistic effect, the effect of which is greatly enhanced (see Example 13).

Accordingly, it is an object of the present invention to provide a sterilizing composition for controlling bacterial plant diseases.

In parallel with the present invention, the following nitrofuran test compounds were used for preliminary and in vitro experiments. :

CH-819, furazolidone = 5-nitro-furfurylten-3-amino-2-oxazolidone:

CH-1237, nitrofurantoin = N- (5-nitro-furfurylidene-2-)-1-amino-hydantoin:

CH-1552 = 3- (5-nitro-furfurylidene-amino) -imidazole-2-thiol:

CH-2007 = 2- (5-Nitro-furfurylidene-amino) -4-chloromethyl-5- (2-chloroethyl) -thiazole:

CH-2011 = 2- (5-Nitro-furfurylidene-amino) -4-methyl-5- (2-chloroethyl) -thiazole:

CH-2103 = 2- (5-nitro-fururilidene-amino) -4-methyl-5- (2-hydroxyxyl) -thiazole.

The results are shown in Table 2.

TABLE 2

Antibacterial Effect of Nitrofuran Derivatives in Aseptic Culture

A => 1000mg / ml

It is well known that Erbinia Carotoborah and Agrobacterium tumefaciens are very sensitive to NO.819 compounds but moderately sensitive to No.1552 compounds.

Based on the preliminary test results, in vitro experiments were conducted to determine the biological activity of the compositions of nitrofuran derivatives and nitrofuran derivatives and antifungal active ingredients.

The following examples are intended to illustrate the invention in more detail, and the invention is not limited to these examples.

TABLE 3

Effect of Carbendazim on Bactericidal Activity of Furazolidone Against Various Bacteria

Carvandazim did not inhibit the growth of any of these bacteria even at a concentration of 9000 mg / ml. The term "nothing" means that the effect of furazolidone is the same as the effect on the control plate without carbendazim.

Example 1

In the Petri-Dish test, Irbinia carotobora var.

Activity of Test Compounds on Atroceptica

Five replicates were performed for each dose using a nutrient medium suitable to isolate Erwinia carotobora.

When the bacteria were bought, they were cultured in agar nutrient medium, and the bacteria were purely separated and inoculated in the nutrient medium cooled to 40-50 ° C as follows.

Pour 20 ml of nutrient medium into a petri-dish, 10 cm direct, and harden, and make four holes per petri-dish. 0.1 mL of suspension was applied to each hole. Incubated at 25 ° C. Table 4 shows the results.

TABLE 4

From the above results, Nitrofuran UDO Nos. CH-819 and CH-1552 were obtained from Erbinia carotobora var. At a concentration of 1.0-1.5 mg / ml and a dose of 0.1 ml / hole. It can be seen that there is activity against atheroseptica. Benomil and MBC did not increase bactericidal activity.

Example 2

Potato tubers (desired species) were bisected.

In each of the two pieces, five holes with a depth of 15 mm and a diameter of 10 mm were drilled at equal distances from each other.

0.1 ml of Erbinia carotobora var. Aqueous suspension of atheroseptica bacteria was added dropwise. After 30 minutes, the test compound was added dropwise to the hole pretreated with bacteria using micropipette. Treatments and infected tubers were left for 72 hours in a humid room to see results. The hole was cut into two parts perpendicular to the initial cut and the weight was scraped off the area. Based on the following criteria, the treatment efficiency was measured in comparison with the untreated control group.

The percentage values above represent the weight of scraped material infected with Erbinia carotoboro stroceptica relative to the control weight.

"0" means very preferred, and "4" means that the treatment effect is very low. The results are shown in Table 5.

TABLE 5

Similar to the Petri-Dish experiment, the nitrofuran derivative No. CH-819 and No. Combined administration of CH-1552 and these compounds with antifungal agents was effective.

The combination of antifungal and antibacterial agents, ie CH-819 + MBC + Ditan M-45 (active ingredient mancozeb) (ratio 50% + 25% + 25%), showed high activity.

The present invention provides compound No. It is based on the fact that CH-819 and CH-1552 show strong activity against Erbinia phytopathogenic bacteria.

The bactericidal composition of the present invention is very suitable for treating bacterial diseases or bacterial and fungal diseases respectively.

Plant protectants can be formulated in many forms, with the composition being selected according to the conditions of administration, plant culture engineering, and the available machinery.

In order to protect tubers, bulbs, roots, etc. during storage, it is necessary to treat the previously propagated material before storage. The foregoing technique is the same or similar to the so-called "dressing process" commonly used for pre-sowing treatment and germination of plant parts. The above spraying methods can be carried out at different times.

Pesticides of the present invention may be formulated in conventional formulations such as powders, powders dispersible in water, dispersant concentrates, granules and the like.

The preparation of the compositions of the present invention consists of two basic processes, which are often used in admixture with each other.

The first step is to prepare the active ingredient in suitable particles. To obtain satisfactory results, 70% of the particles must have a particle size of less than 10 µm. This process can be done in two ways. According to this method, the active ingredient is ground and particles of the desired size are produced by a drying process (eg airflow generator) or a wet process (eg pearl or sand mill). The chemical reaction for producing the active line powder is preferably carried out in the same manner as the method for obtaining the active ingredient having the desired particle size. However, it is unnecessary to use an expensive grinder which requires high energy.

The second process is to prepare the desired formulation, wherein the particles of the active ingredient are contacted with adjuvants, carriers, diluents, adhesives, activity increasing compounds, colorants and the like.

Dispersible Powder In Water

Essentially, the particles of the active ingredient must be readily dispersible in water, and when administered to the plant to be protected, the dispersant must be coated uniformly. Such hydrol mixtures should contain so-called wetting agents. The chemical structure of the wetting agent should consist of polar and nonpolar residues. Examples of the wetting agents described above are as follows.

Anionic active compound

References to the above supplements are as follows.

Kurt Lindner, Tenside, Textilhilfsmittel, Waschrohstoffe, Wissenschaftliche Verlaggesellschaft MbH, Stuttgart.

Dispersants formed from particles of the wetted and dispersed active ingredient at the time of use must be stable, for example, during storage, and no sedimentation causing a change in concentration should occur. This problem can be solved by using a suitable dispersant. The foregoing dispersants are polymeric substances that act as protective colloids. For this purpose, calcium, sodium or ammonium salts of lignin sulfonate, hydrated polymers, mucus and the like are used.

Crushed minerals such as china-clay, kaolin, bentonite, calcium carbonate, gypsum and the like are used as the carrier or filler.

The hydrated powder composition includes, of course, an adhesive. As the aforementioned compound, a water-soluble macroorganic polymer is particularly preferable.

Representative examples of the foregoing compounds are as follows:

Carboxymethylated starch, carboxymethylated cellulose, polyvinylpyrrolidone.

In order to increase the effect, the compositions of the present invention include inorganic salts such as sodium sulfate, organic acids (tartaric acid), vegetable oils and mineral oils, and the like.

In order to improve the storage properties, the composition includes synthetic silicic acid, aluminum oxide and the like having a large surface area.

For the user, foaming is a serious problem. This problem is solved by using a foam inhibitor. For this, use an oil dispersant. The aforementioned foam inhibitor is prepared by mixing a suitable emulsifier system selected with oil in consideration of the oil to be emulsified and pouring the resulting mixture into water and stirring with a suitable stirrer. If water is an unwanted component in the mixture, the oil constituting the emulsifier may be added to the composition. In this case, the effect of oil is seen when the active ingredient is added to the suspension. Foam inhibitors should have the property of emulsifying themselves. As antifoam, suspensions of plant or mineral oils, in particular suspensions of silicone oils, can be used.

Foam inhibitors can also be obtained by partially saponifying plant or mineral oils with sodium or potassium hydroxide. The advantage of the stable suspension thus obtained is that it is easily and completely degraded during the biological life of the plant.

Suspension concentrates exhibit a rapidly elevated effect in plant protection technology. The above composition comprises the active ingredient in the form of a suspension and an adjuvant in the form of an aqueous and / or oily liquid. Water and / or oil act as fillers (excipients) as well as solvents. Suspension concentrates have the following characteristics:

1. The above composition is a suspension, so the disadvantage of powder formation is reduced.

2. When suspending the powder composition, slow hydration due to the weak hydration properties of the active powder particles is a serious problem.

However, this drawback does not arise in the case of suspension concentrates.

3. Auxiliaries that improve adhesion and other properties are present in the solution to prevent agglomeration, so that the nozzles are not clogged when spraying and can be sprayed constantly.

4. The composition exhibits an excellent floating effect.

Suspension concentrates generally consist of a number of wetting and dispersing agents in combination with a hydrated powder. The foregoing suspension concentrates also contain substances which increase the stability of the suspension, ie, water soluble macromolecules. To this end, for example, carbohydrate derivatives, synthetic polymers, substances which improve the structure of the suspension (eg, artificial synthetic aluminum silicate oxide with large surface area and bentonite, organophilic bentonite, etc.) may be used.

Freezing point synergists (freezing inhibitors) are important components of aqueous suspensions. For this purpose, preference is given to using polyols, in particular ethylene glycol.

Use of suspensions of plant, animal or mineral oils in suitable proportions preferably affects the stability of the suspension. The aforementioned additives, of course, improve the biological stability of the composition.

So-called soil sterilization methods can suppress soil microorganisms. For this purpose, it is preferable to use granule disinfectants. When the composition described above is mixed with the soil, the active ingredient is released by the moisture, and it is located here and there in the soil, thereby killing unwanted parasites, thereby creating an environment suitable for growing cultivated plants.

Active ingredients, carriers, fillers and auxiliaries (e.g. wetting agents, dispersants, adhesives, delivery control agents, disintegrating agents, etc.) that have been ground to a suitable particle size are mixed with water, the mixture is kneaded and the resultant is pressed to give a constant Sterile granules are prepared by passing through a sieve of the mesh or by granulation in a known manner and drying and crushing or spray-drying. Another method is to arrange in advance a carrier of a desired particle size (eg organic or inorganic granules) and apply the active ingredient particles to the surface of the carrier. If one or all of the active ingredients is a liquid, carrier particles with a suitable porosity are used, and the liquid is adsorbed onto the above porous particles. When the active ingredient is partially solid and partly liquid, the solid active ingredient may be dispersed in the liquid active ingredient, and the liquid thus obtained may be absorbed into the porous carrier, and the solid particles adhere to the carrier. When using a soluble active ingredient, it is preferable to prepare a solution from the active ingredient, absorb the obtained solution into a carrier and evaporate the solvent.

It is preferable to project natural materials (eg cellulose derivatives, sugars, gelatin, gums, plants, animals or mineral oils, fats, etc.) as adhesives. Synthetic adhesives (e.g. polyvinyl alcohol, polyvinylacetate, polyacrylic acid, polyacrylamide, polyvinylpyridone, etc.) may also be used.

Based on in vitro experiments, in vivo experiments were conducted to determine antibacterial and antifungal effects. The composition used in the above experiment was prepared according to the following example.

Example 3

Active ingredients having the following particle size distributions were prepared:

10μm or more 20.3%

5-10㎛ 31.0%

5 µm or less 48.7%

The measurement was carried out using an ultrasonic macroshift set (manufactured by Retsch).

100 kg of active ingredient (CH-819), 3 kg of diotilane, 5 kg of Totanin B, 41 kg of Neuburgch choke and 1 kg of Tylose C were weighed in a 600 L Loedige type high speed stirrer through a pulverizing granulator. The mixture was stirred for 10 minutes and ground to 200 kg / hr in 315 mill of Alpine so that the components were in good contact. The mixture was uniformed and finally distributed in 300 L Nautamix and the product packaged.

The floating capacity of CH-819 80wp thus obtained reached 89%. (Measured in 0.5% suspension according to WHO standard).

Example 4

The following composition was prepared using the CH-819 active ingredient having a particle size distribution of Example 3:

The hydrated powder composition thus obtained is particularly suitable for treating bacterial infections of potato and root plants because the composition contains very high active ingredients.

Example 5

In order to effectively suppress fungal and bacterial simultaneous infection, in addition to antibacterial agents, systemic antifungal agents can be added to the composition:

The ingredients were added to a 600 L Rhodige high speed stirrer through a milling granulator. Genapol PGM was excluded because it is a highly viscous wetting agent with a water content of 50%. The mixture was stirred for 3 minutes and Gennapol was added directly. That is, it did not pass through the milling granulator. It was homogenized for 10 minutes. The mixture was dried in a KFSZ-250-SH type liquefaction dryer with a stirrer. The mixture was triturated in an Alpine AS-200 ventilator and again homogenized and packaged in a 300L Nautamix stirrer.

Example 6

The following composition was prepared:

The mixture was triturated in an Alpine AS-200 ventilator to give an average particle size of 5 μm, and completely homogenized and packaged with dimethyl sulfoxide in a 300 L Nautamix stirrer.

Example 7

Water (183.35 g) was mixed with ethylene glycol (55 g). The resulting mixture dissolved Tensiopix B 4725 (16.5 g) and Tensiopix CG 11 (16.5 g) at 40 ° C. In the solution thus obtained, CH-819 (143 g) having the same particle size as in Example 3 was dispersed using a stirrer (ultraturax).

A mixture of paraffin oil (99 g) and Triton X-45 (11 g) was added to the suspension with dispersion stirring. The oil-solid-water system was stirred until uniform. 2% (weight ratio) of the aqueous solution of Tensiopix 821 (27,5g) was added stirring. Since foaming occurs when stirring, Tenciopix L 0 5 1 (1 ₀ g) was added as a foam inhibitor at the end of this step. The composition thus obtained adheres well to plant parts. The suspension has good stability and the floating capacity is more than 95%.

Example 8

A solvent was used to mix water (228.3 kg) and ethylene glycol (7 kg). The following components were dissolved in the solution thus obtained while stirring:

Atrox 4875: 1.5 kg

Atrox 1225: 0.5kg

Atrox 4852 / b: 1.5 kg

In the above surfactant solution, the active ingredients of CH-819 (21 kg) and mancozeb (34 kg) were suspended using a decontamination agent.

The suspension was triturated at 200 L / hr in a 50 L pearl mill to give an average solid particle size of 5 μm or less. Paraffin oil (9 kg) and Triton X45 (1 kg) were added to the suspension with stirring in an apparatus with a stirrer. After thoroughly mixing, a 2% aqueous solution of Tensiopix 821 (5 kg) was added while stirring.

The mixture was stirred and stabilized through a suspension suspension system obtained. At the end of the process, a foam inhibitor Tensiooffice L051 (0.3 kg) was added prior to final mixing. The resulting mixed sterilization mixture has good spraying properties. The composition adheres better to each part of the plant better than conventional fungicides.

Example 9

The following sculpture was prepared.

20 parts MBC by weight 10 parts ethylene glycol by weight

20 parts by weight of CH-819, 2 parts by weight of a 2% aqueous solution of Tensiooffice 821

Tensiopix B 4725 by weight 3 parts Water by weight 40 parts

Ten parts of Tencio fix CG 21 by weight

Example 10

Effect of CH-819 on Infected Parasites (Bacteria and Fungi) During Potato Storage

종)을 저장전에 처리했다.Potato tubers (consumer

Species) were treated before storage.

25 kg of tubers were used for each trial. The number of duplicate trials is five. The tubers are stored in a potato storehouse until the results are measured. The number of bad tubers and diseased tubers was calculated.

The results are shown in Table 6.

TABLE 6

Compositions containing only nitrofuran derivatives as active ingredients are active only in Ervinia. However, pain disorders caused by Fusarium and Alternaria can be suppressed by using compositions containing antifungal agents in addition to nitrofuran derivatives. The compositions of Examples 5, 6 and 9 are very effective in inhibiting infections, and in particular the compositions of Example 6 (comprising 13 + 34 + 10% by weight of CH-819 + benomil + mancozeb) have the best results. Indicated.

Example 11

After the potato was harvested, the propagable tubers of UT-1 (desired species) were treated with a certain amount of test compound in the form of wet dressing on October 23 (treated solution 15L). Potatoes were harvested by machine, washed and sorted before processing. Lumps and diseased tubers were removed. 200 tubers were used for each treatment and repeated 3 times with each dose. The treated tubers were placed in bags made of cloth and stacked in a ventilated storage device. Storage temperature was below 15 ℃ and relative humidity content was 80-90%. The environmental conditions (temperature, water content) of the storage device were adjusted to create conditions suitable for the growth of pathogenic parasites.

The results were measured on April 30 the following year. The weight and ratio of normal and diseased tubers, the degree of disease and the degree of infection of parasites were determined.

The results are shown in Table 7.

TABLE 7

* Reference DD-PS 209950

The above industrial scale test results demonstrate that the composition of the present invention is effective against plant diseases. In the untreated control group, Fusarium dry rot disease causes severe loss, and Erbinia carotobora var. Atrovceptika is as bad as that.

The compositions of Examples 5 and 9 are particularly effective for Fusarium-ervinia, and the compositions of Example 6 are very good for Fusarium + Erbinia + Alternaria.

Example 12

Before seeding, the treatment machine Kumotox was used to treat the test compound with a capacity of 6.5 liters of the treating solution. Seeding was carried out using a 4-SaBP-75 planter. The sample size was 0.5 ha and the number of duplicate runs was 3. Seed tubers weighed 2.8 kg / ha.

Fifteen days after sowing, the number of non- sprouting plants was counted as much as the result of Lizatonia infection. In 4 × 50 (m) specimens, shoots infected with liqtonia were counted. (200 shoots each). The number of infected tubers was counted by examining each 200 tubers. The proportion of tubers with a seed size of 4-6 cm was calculated. The results are summarized in Table 8.

TABLE 8

As a result of spraying before sowing, the yield and potato yield increased significantly.

Example 13

Sensitivity of Plant Pathogens and Bacteria when Carbendazim and Furazolidone are Treated with Mixtures in Various Ratios

In vitro bacterial tests were performed. The minimum blocking concentrations of the various mixtures of the two antimicrobial substances described above were determined as follows.

Mixtures of various proportions of the two marine products were prepared and mixed with influence media of the composition as follows. Final concentrations of furazolidone were 1000, 100, 10, 1 and 0.1 mg / l, respectively. The concentration of carbendazim in the medium varied with the mixing ratio.

b 1981 : Biometrical methods in research, Mez

gazds

gi Kiad

Budapest]에 의거하여 결과를 계산했다.For each test species (25 species), the relative efficiency with respect to furazolidone was calculated according to each mixing ratio. McKinney's Official [Mckiney Sv

b 1981: Biometrical methods in research, Mez

gazds

gi Kiad

Budapest] was calculated.

The results are shown graphically in FIG.

Surprisingly, the presence of large amounts of carbendazim in the mixture significantly increases the antibacterial activity of furazolidone. In other words, it may increase by 100 times (see Table 3). This synergy is of particular interest in the genus Santomonas.

Nutrition medium

Agar powder nutrition medium (Human OEV) 26g: Bactobacillus 2.5g: Glucose 3g: Yeast Extract 1g: Bactoa peptone 2g: Potassium dihydroge-phosphate 0.55g: Potassium chloride 0.1g: Sodium chloride 0.1g: Magnesium sulfate 0.125 g: calcium chloride 0.125 g: iron citrate 0.02 g: microelement solution by Hogland and Anon's method 1 ml: total amount of nutrient medium is 1 l. 2N ammonia water was added to adjust the pH to 6.5.

Inoculation

Test compounds were added to the nutrient medium (temperature: 50 ° C.) before making the plates. The medium was hardened in the usual manner and then inoculated.

Example 14

Inhibition of infection with your Santomonas Orissa by seed-spray method

The pathogens were isolated from the rice seeds of Far East Asia, whose infection is unknown. Treated seeds. Prior to germination, a mixture of Colfugo 25FW and Furazolidine 30FW was treated with 250 seeds at each dose at appropriate doses. (Example 7). Corfugo 25FW was inactive for purely cultured bacteria and was not used for seed treatment. Seeds are pre-germinated for 48 hours in damp filter paper (so-called dopog method). A few sprouted 200 seeds were selected for each treatment. The stems of the radicles of these seeds were punctured with a needle to make holes. The seeds thus treated were planted in sterile soil and grown on phytotrons for 15 days. (12 hours sunshine, 28 ℃ during the day, 25 ℃ at night).

The results are shown in Table 4.

TABLE 4

Claims (3)

The active ingredient contains 1-99% (by weight) of the total amount of nitrofuran derivative of general formula (I) and any one or more antifungal active ingredients, and in the case of two active ingredients, a general structural formula for a known antifungal active ingredient The ratio of the active ingredient of (I) is 1: 99-99: 1, preferably 1: 9-9: 1 and: 1: 3 in the case of the composition having three active ingredients: 1: 9: 0.5-1: 1: 5, Preferably 1: 4: 5, preferably 1; 4: 1-1: 1: 4, and each of these compositions is used in admixture with a suitable inert solid or liquid carrier or diluent and conventional auxiliaries (eg wetting agents, dispersants, adhesives, antifoams and / or cryoprotectants). A bactericidal composition which is active against bacterial plant diseases.

In the formula, X means structural formula (a) or (b).

2. In the first term, a composition containing as an additional antifungal active ingredient one or more of the following compounds: 1-Butylcarbamoyl-benzimidazole-2-methyl-carbamate: 2-methoxycarbonylamino-benzimidazole: 1, 2-bis- (3-methoxycarbonyl-thioureido) benzene: 2- (4-thiazolyl) benz imidazole: Zinc Ethylene-Bis-Dithiocarbamate: Manganese-ethylene-bis-dithiocarbamate: Tetramethyl-thiuram-disulfide: N-tricholomethylthio-tetrahydro-phthalimide. 2. A composition active in bacterial plant disease according to claim 1, wherein the compound of formula (I) and any one or more known antifungal active ingredients are mixed with a suitable inert solid or liquid carrier or diluent and any suitable excipients. Manufacturing method.

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