KR870005248A - Methods and Reagents for Quantifying Bindable Analytes - Google Patents

Methods and Reagents for Quantifying Bindable Analytes Download PDF

Info

Publication number
KR870005248A
KR870005248A KR860009308A KR860009308A KR870005248A KR 870005248 A KR870005248 A KR 870005248A KR 860009308 A KR860009308 A KR 860009308A KR 860009308 A KR860009308 A KR 860009308A KR 870005248 A KR870005248 A KR 870005248A
Authority
KR
South Korea
Prior art keywords
receptor
solid phase
analyte
measurement
phase
Prior art date
Application number
KR860009308A
Other languages
Korean (ko)
Inventor
피터 카스파 클라우스
Original Assignee
독토르 다움 및 독토르. 웨버
뵈링거 만하임 게엠베하
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 독토르 다움 및 독토르. 웨버, 뵈링거 만하임 게엠베하 filed Critical 독토르 다움 및 독토르. 웨버
Publication of KR870005248A publication Critical patent/KR870005248A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/819—Multifunctional antigen or antibody
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/824—Immunological separation techniques
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

1. Process for the determination of a bindable analyte according to the principle of heterogeneous immunoassay by incubation of a sample solution which contains the analyte with a labelled first receptor specifically bindable with the analyte and present in dissolved phase and a second receptor present in a solid phase which does not cross-react with the first receptor and can fix a complex which contains the analyte and first receptor, separation of the phases after incubation and quantitative measurement of the labelling bound to the solid phase, characterised in that one determines the back dissociation velocity of the labelling bound to the solid phase into the dissolved phase and uses the quotients of back dissociation velocity and measurement value as measure for the correctness of the test result of the first measurement.

Description

결합가능한 분석물을 정량하는 방법 및 시약Methods and Reagents for Quantifying Bindable Analytes

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 분석물의 농도에 따르는 흡광도 곡선이고, 굵은선은 분석물의 농도 정량에 대한 계산곡선이다.1 is the absorbance curve according to the concentration of the analyte, and the thick line is the calculation curve for quantifying the concentration of the analyte.

제2도는 본 발명을 행하는데 사용하는 원심분리 자동분석기의 삽입요소이다.2 is an insertion element of a centrifugal autoanalyzer used in practicing the present invention.

Claims (8)

분석물을 포함하는 샘플 용액을 용해된 상에 존재하고 분석물과 특이적으로 결합 가능한 표식된 제1의 수용기 및 제1의 수용기와 반응되지 않고 분석물 및 제1의 수용기를 포함하는 복합체를 고정가능한 고체상에 존재하는 제2의 수용기와 배양하고, 상을 분리하고, 고체 상에 결합된 표식양을 측정함에 의해서 이질의 면역분석 원리에 따라 결합가능한 분석물을 정량하는데, 여기에서 용해된 상으로 고체상에 결합된 표식의 역 분해속도 및 역 분해 속도의 지수가 결정되고 측정치는 첫번째 측정의 테스트 결과 정확성에 대한 측정으로 사용되는 방법.Fixing the complex comprising the analyte and the first receptor without reacting the labeled first receptor and the first receptor present in the dissolved phase and specifically binding to the analyte, the sample solution comprising the analyte By culturing with a second receptor present in a possible solid phase, separating the phases, and measuring the amount of label bound to the solid phase, quantitatively bindable analytes are carried out according to heterogeneous immunoassay principles, wherein A method of determining the inverse decomposition rate and inverse decomposition rate of a marker bound to a solid phase and the measurement being used as a measure for the accuracy of the test results of the first measurement. 제1항에 있어서, 역 분해 속도가 첫번째 측정에 대한 제한된 시간 간격에서 고체상에 결합된 표식의 최소한 하나 제2의 측정에 의해 결정되는 방법.The method of claim 1, wherein the rate of reverse decomposition is determined by at least one second measurement of the marker bound to the solid phase at a limited time interval for the first measurement. 제1항 또는 제2항에 있어서, 효소로 표식된 제1의 수용기를 사용하고, 고체상은 표식효소를 정량하는 색체 시약과 배양하고, 시약 용액은 고체상으로 부터 분리하고, 역분해 속도는 제한된 시간 간격에서 최소한 두 측정으로 시약 용액내에서 결정되는 방법.The method according to claim 1 or 2, wherein the enzyme-labeled first receptor is used, the solid phase is incubated with a chromosome reagent to quantify the marker enzyme, the reagent solution is separated from the solid phase, and the rate of reverse decomposition is limited. Method determined in reagent solution with at least two measurements at intervals. 제1항 또는 제2항에 있어서, 첫번째 측정 후에, 고체 상을 액체 상과 다시 배양하고 역 분해 속도는 고체 상에 남아 있는 표식 또는 액체 상에 통과된 표식을 측정하여 결정하는 방법.The method according to claim 1, wherein after the first measurement, the solid phase is incubated again with the liquid phase and the reverse decomposition rate is determined by measuring the label remaining on the solid phase or the label passed through the liquid phase. 전기한 항의 어느 한항에 있어서, 제1의 수용기가 조종된 것에 대한 것과 다른 분석물의 정량 인자에 대해 조종된 분석물에 대해 특이한 제3의 수용기가 부가되고 제2의 수용기가 제3의 수용기와 결합가능한 방법.The method of any one of the preceding claims, wherein a third receptor specific for the analyte steered for the first receptor and for the analyte steered for the quantitative factor of the other analyte is added and the second receptor is coupled to the third receptor. Possible way. 제5항에 있어서, 모노크론 항체가 제3의 수용기로서 사용되고, 제1의 수용기가 모노크론 항체의 Fab단편을 포함하고 제2의 수용기가 제3수용기의 Fc부분과 결합가능한 방법.The method of claim 5, wherein the monoclonal antibody is used as the third receptor, the first receptor comprises a Fab fragment of the monoclonal antibody and the second receptor is capable of binding to the Fc portion of the third receptor. 전기한 항의 어느 한 항에 있어서, 시약 내 최소한 한 수용기의 역 분해 상수가 결합 상수에 따르는 항체의 선택 및 / 또는 세정제 및 / 또는 무질서를 유발하는 이온의 부가에 의해 조정되는 방법.The method of claim 1, wherein the reverse degradation constant of the at least one receptor in the reagent is adjusted by the selection of the antibody according to the binding constant and / or by the addition of ions causing cleaning and / or disorder. 제7항에 있어서, 고체 상에 결합된 표식의 최소한 10%가 다시 분해되는 측정 시간에서, 최소한 하나의 분석물 농도에서 최소한 한 수용기의 역 분해 상수가 선택되는 방법.8. The method of claim 7, wherein a reverse decomposition constant of at least one receptor at at least one analyte concentration is selected at a measurement time at least 10% of the marker bound to the solid phase is degraded again. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR860009308A 1985-11-05 1986-11-05 Methods and Reagents for Quantifying Bindable Analytes KR870005248A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19853539215 DE3539215A1 (en) 1985-11-05 1985-11-05 METHOD FOR DETERMINING AN IMMUNOLOGICALLY BINDABLE ANALYT
DE3539215.0 1985-11-05

Publications (1)

Publication Number Publication Date
KR870005248A true KR870005248A (en) 1987-06-05

Family

ID=6285208

Family Applications (1)

Application Number Title Priority Date Filing Date
KR860009308A KR870005248A (en) 1985-11-05 1986-11-05 Methods and Reagents for Quantifying Bindable Analytes

Country Status (7)

Country Link
US (1) US4966839A (en)
EP (1) EP0227921B1 (en)
JP (1) JPS62112064A (en)
KR (1) KR870005248A (en)
AT (1) ATE50459T1 (en)
DE (2) DE3539215A1 (en)
DK (1) DK163022C (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3718140A1 (en) * 1987-05-29 1988-12-08 Boehringer Mannheim Gmbh HETEROGENIC IMMUNOASSAY
US5856194A (en) 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
US5795784A (en) 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
DE19808226C1 (en) * 1998-02-27 2000-03-02 Bruker Analytik Gmbh Investigating hydrophilic macromolecules in an aqueous solution by attaching them to a lipid film on a solid carrier
US6284472B1 (en) * 1998-10-05 2001-09-04 Dade Behring Inc. Method for extending the range of an immunoassay
CA2418861A1 (en) * 2000-08-11 2002-02-21 Nanotype Gmbh Method and device for characterising and/or for detecting a bonding complex
DE10126798A1 (en) * 2001-06-01 2002-12-19 Nanotype Gmbh Highly parallel determination of analytes, useful e.g. for diagnostic detection of proteins, by immobilization then contacting with surface carrying specific probes
US20030232340A1 (en) * 2002-06-13 2003-12-18 David Anderson Nanoporous particle with a retained target
WO2006063174A2 (en) * 2004-12-08 2006-06-15 Lyotropic Therapeutics, Inc. Compositions for binding to assay substrata and methods of using

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4329213A (en) * 1977-07-14 1982-05-11 Elwing Hans B Gel diffusion immunoassay including α-feto protein determination
US4381291A (en) * 1979-02-23 1983-04-26 Ab Fortia Measurement of free ligands
JPS57136165A (en) * 1981-02-18 1982-08-23 Mochida Pharmaceut Co Ltd Immunological measuring reagent
US4459359A (en) * 1981-11-19 1984-07-10 New York Blood Center, Inc. Sensitive immunoassays of antigens or antibodies sequestered within immune complexes
FR2519763A1 (en) * 1982-01-14 1983-07-18 Guigan Jean PACKAGING DEVICE FOR MULTIPLE ANALYSIS
US4595661A (en) * 1983-11-18 1986-06-17 Beckman Instruments, Inc. Immunoassays and kits for use therein which include low affinity antibodies for reducing the hook effect
DE3425008A1 (en) * 1984-07-06 1986-02-06 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD AND DEVICE FOR CARRYING OUT ANALYTICAL PROVISIONS
FR2579756B1 (en) * 1985-03-26 1987-05-07 Guigan Jean METHOD FOR PERFORMING BIOLOGICAL ANALYSIS USING IMMUNOLOGICAL REACTIONS AND DEVICE FOR CARRYING OUT SAID METHOD

Also Published As

Publication number Publication date
DE3669078D1 (en) 1990-03-29
DK528286D0 (en) 1986-11-05
DK163022B (en) 1992-01-06
EP0227921B1 (en) 1990-02-21
JPS62112064A (en) 1987-05-23
DE3539215A1 (en) 1987-05-07
US4966839A (en) 1990-10-30
ATE50459T1 (en) 1990-03-15
JPH0476628B2 (en) 1992-12-04
DK528286A (en) 1987-05-06
DK163022C (en) 1992-06-01
EP0227921A1 (en) 1987-07-08

Similar Documents

Publication Publication Date Title
Gosling A decade of development in immunoassay methodology
Norden et al. Misleading results from immunoassays of serum free thyroxine in the presence of rheumatoid factor
Wauthier et al. Interferences in immunoassays: review and practical algorithm
KR910008407A (en) Improved automated immunochromatography device
US5573921A (en) Immunochemical displacement for determining an analyte
RU95118163A (en) CHROMATOGRAPHIC ANALYSIS DEVICE
DE69714584D1 (en) IMMUNOASSAYS FOR DETECTING ANTIPHOSPHOLIPID ANTIBODIES
CA2437618A1 (en) Urine assay for ovarian reserve
DK406087A (en) METHOD AND TEST KIT FOR DETERMINING THE CONCENTRATION OF AN ACTIVE RELATION IN A BIOLOGICAL SCIENCE
KR870005248A (en) Methods and Reagents for Quantifying Bindable Analytes
JPS6447951A (en) Immunoassay method and kit for hapten detection by agglutination
US6087088A (en) Binding assays using more than one label for determining analyte in the presence of interfering factors
ES8609723A1 (en) Method for immunologically measuring antigens, antibodies or haptens, the use of this method for measuring lipoproteins and alpha-fetoprotein and test kits for the performance of this method.
Lin et al. Determination of the dissociation constant of phosvitin–anti-phosphoserine interaction by affinity capillary electrophoresis
US5306622A (en) Heterogeneous immunoassay process
ES462365A1 (en) Method and apparatus for the detection of a specific binding protein
DE58907087D1 (en) Method for the determination of antibodies specific for a class of antigen and reagent suitable therefor.
ES2125982T3 (en) TURBIDIMETRIC IMMUNOLOGICAL TEST OF INITIAL SPEED.
KR880013013A (en) Quantitative reagents and immunochemical methods for the determination of multivalent antigens in liquid samples
Wu et al. Matrix interference in serum total thyroxin (T4) time-resolved fluorescence immunoassay (TRFIA) and its elimination with the use of streptavidin–biotin separation technique
US4732848A (en) Process for the determination of an immunologically-bindable substance involving a Fab fragment
JPS635265A (en) Method for immunoassay
WO2002014869A3 (en) Assay method and device for detecting the presence and concentration of analytes
JPS62294964A (en) Immunological analysis
DE59610859D1 (en) Immunological method of determination

Legal Events

Date Code Title Description
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid