KR850007565A - 정액분리방법 - Google Patents

정액분리방법 Download PDF

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Publication number
KR850007565A
KR850007565A KR1019850002661A KR850002661A KR850007565A KR 850007565 A KR850007565 A KR 850007565A KR 1019850002661 A KR1019850002661 A KR 1019850002661A KR 850002661 A KR850002661 A KR 850002661A KR 850007565 A KR850007565 A KR 850007565A
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KR
South Korea
Prior art keywords
medium
osmosis
semen
density
low
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KR1019850002661A
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English (en)
Inventor
셔림톤 왈라스
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셔림톤 왈라스
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Publication date
Application filed by 셔림톤 왈라스 filed Critical 셔림톤 왈라스
Publication of KR850007565A publication Critical patent/KR850007565A/ko

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
    • B03B5/00—Washing granular, powdered or lumpy materials; Wet separating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
    • B03B5/00—Washing granular, powdered or lumpy materials; Wet separating
    • B03B5/28—Washing granular, powdered or lumpy materials; Wet separating by sink-float separation
    • B03B5/30—Washing granular, powdered or lumpy materials; Wet separating by sink-float separation using heavy liquids or suspensions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03D—FLOTATION; DIFFERENTIAL SEDIMENTATION
    • B03D3/00—Differential sedimentation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Zoology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Centrifugal Separators (AREA)
  • External Artificial Organs (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음

Description

정액분리방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (5)

  1. 밀도분리방법의 포노타이픽 디퍼런스에 의해 X정액과 Y정액을 분리하기 위한 방법에서, 정액은 관의 하부상에서는 고밀도를 관의 상부에서는 저밀도를 가지는 양분매질에 현탁되고, 상기 관내의 삼투성구배를 유지하는 구성을 특징으로 하는 정액분리방법.
  2. 제1항에 있어서, 역삼투성은 매질의 하부에서는 비교적 저삼투성을 그리고 매질의 상부에는 고삼투성을 제공함에 의해 유지됨을 특징으로 하는 정액분리방법.
  3. 생물학적물질의 비중분리를 위한 매질을 제조하기 위한 방법에 있어서, a. 저밀도의 제1양분매질을 제공하고 바람직한 소정의 값에 삼투성을 조절하는 과정과, b. 고밀도의 제2양분 매질을 제공하고 상기 제1영양매질의 삼투성보다 더 낮은 값에 삼투성을 조절하는 과정과, c. 하부에는 고밀도 저삼투성을 가지며 상부에는 저밀도 고삼투성을 가지는 비중분리수단을 제공하기 위해 관내에 상기 제1, 제2영양 매질을 가라앉히는 과정으로 구성됨을 특징으로 하는 비중분리용 매질을 제조하기 위한 방법.
  4. 제3항에 있어서, 상기 생물학적 물질은 정액임을 특징으로 하는 비중분리용 매질을 제조하기 위한 방법.
  5. 정액의 비중분리용 매질에 있어서, 상기 매질은 하부에서는 고밀도 저삼투성을 그리고 상부에서는 저밀도 고삼투성을 가짐을 특징으로 하는 비중분리용 매질을 제조하기 위한 방법.
    ※ 참고사항 : 최초출원내용에 의하여 공개하는 것임.
KR1019850002661A 1984-04-20 1985-04-18 정액분리방법 KR850007565A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/602,461 US4605558A (en) 1984-04-20 1984-04-20 Process for cell separation
US602461 1984-04-20

Publications (1)

Publication Number Publication Date
KR850007565A true KR850007565A (ko) 1985-12-07

Family

ID=24411446

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019850002661A KR850007565A (ko) 1984-04-20 1985-04-18 정액분리방법

Country Status (7)

Country Link
US (1) US4605558A (ko)
EP (1) EP0159044A3 (ko)
JP (1) JPS6122851A (ko)
KR (1) KR850007565A (ko)
AU (1) AU4079085A (ko)
BR (1) BR8501891A (ko)
NZ (1) NZ211761A (ko)

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JPH01206610A (ja) * 1988-02-13 1989-08-18 Kitamura Kiden Kk 段付巻鉄心用帯材の切抜方法
US5188305A (en) * 1988-09-27 1993-02-23 Kitamura Kiden Co., Ltd. Apparatus for cutting winding strips for use in a wound core
JPH0289304A (ja) * 1988-09-27 1990-03-29 Kitamura Kiden Kk 巻鉄心用帯材の切抜方法
JPH0541327A (ja) * 1991-08-05 1993-02-19 Denki Tetsushin Kogyo Kk 巻鉄心の製法
JP2901413B2 (ja) * 1992-04-22 1999-06-07 北村機電株式会社 巻鉄心用帯材の切り抜き装置
JP2838627B2 (ja) * 1992-09-03 1998-12-16 電気鉄芯工業株式会社 巻鉄心
JP2771109B2 (ja) * 1994-03-16 1998-07-02 北村機電株式会社 巻鉄心
US5514537A (en) * 1994-11-28 1996-05-07 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Process and apparatus for sorting spermatozoa
US5879877A (en) * 1996-02-09 1999-03-09 Advanced Reproduction Technologies Inc. Use of arabinogalactan in a sperm wash product
US5897987A (en) 1996-03-25 1999-04-27 Advanced Reproduction Technologies, Inc. Use of arabinogalactan in cell cryopreservation media
JP4323571B2 (ja) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド 光学装置
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
EP2283848A1 (en) 1998-07-30 2011-02-16 Xy, Llc Equine system for non-surgical articificial insemination
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
HUP0302232A2 (hu) 2000-05-09 2003-10-28 Xy, Inc. Berendezés és eljárás X-kromoszómát hordozó hímivarsejtek és Y-kromoszómát hordozó hímivarsejtek szétválogatására, eljárás részecskék megkülönböztetésére és részecskeosztályozó berendezés
AU2002220018A1 (en) 2000-11-29 2002-06-11 Colorado State University System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
EP1348222A2 (en) * 2000-12-29 2003-10-01 ABB Technology AG Method of manufacturing a stacked core for a magnetic induction device
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
MX337304B (es) 2002-08-01 2016-02-24 Xy Llc Sistema de separacion de baja presion para celulas de esperma.
WO2004017041A2 (en) 2002-08-15 2004-02-26 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
EP2306174B1 (en) 2003-03-28 2016-05-11 Inguran, LLC Flow cytometry nozzle for orienting particles and corresponding method
NZ544103A (en) 2003-05-15 2010-10-29 Xy Llc Efficient haploid cell sorting for flow cytometer systems
CN104974983A (zh) 2004-03-29 2015-10-14 英格朗公司 用于授精的精子悬浮液
AU2005266930B2 (en) 2004-07-22 2010-09-16 Inguran, Llc Process for enriching a population of sperm cells
US7618770B2 (en) 2005-07-29 2009-11-17 Xy, Inc. Methods and apparatus for reducing protein content in sperm cell extenders
CN102907417B (zh) * 2012-10-22 2014-01-29 西北农林科技大学 一种用于提高荷斯坦公牛x精子冻后品质的冷冻稀释液配方

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4327177A (en) * 1969-04-10 1982-04-27 Wallace Shrimpton Method and means for controlling the sex of mammalian offspring and product therefor
US3894529A (en) * 1969-04-10 1975-07-15 Bio Controls Inc Method and means for controlling the sex of mammalian offspring and product therefor

Also Published As

Publication number Publication date
AU4079085A (en) 1985-10-24
BR8501891A (pt) 1985-12-24
EP0159044A3 (en) 1987-09-16
NZ211761A (en) 1989-08-29
EP0159044A2 (en) 1985-10-23
US4605558A (en) 1986-08-12
JPS6122851A (ja) 1986-01-31

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