KR840005824A - 바이러스성 b형 간염 진단을 위한 시약의 제조방법 - Google Patents

바이러스성 b형 간염 진단을 위한 시약의 제조방법 Download PDF

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KR840005824A
KR840005824A KR1019830003808A KR830003808A KR840005824A KR 840005824 A KR840005824 A KR 840005824A KR 1019830003808 A KR1019830003808 A KR 1019830003808A KR 830003808 A KR830003808 A KR 830003808A KR 840005824 A KR840005824 A KR 840005824A
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에밀 투이 뒤에이유 쟝
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원본미기재
쏘씨에떼 아노님 사노피
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    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/82—Hepatitis associated antigens and antibodies

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Abstract

내용 없음

Description

바이러스성 B형 간염 진단을 위한 시약의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (9)

  1. 하기의 성분을 함유함을 특징으로 하는 평균 직경이 약 10nm∼약 10㎛이고, 일정한 크기를 갖는 다작용 친수성 구상미립자.
    1) R1이 C1∼C4를 갖는 알킬기인 식의 아크릴릭 알데히드의 공중합으로부터 얻어지는 구성단위의 5∼95중량%, 바람직하게는 40∼60중량%,
    2) R2가 수소원자 및 C1∼C4를 갖는 알킬기 중에서 선택되고, R3가 저급알킬기인 식의 아크릴산 유도체의 공중합으로부터 얻어지는 구성단위의 5∼90중량%, 바람직하게는 40∼70중량%,
    3) R4, R5및 R6는 같거나 또는 다른 저급알킬기 (C1∼C4)인및의 유도체 중에서 선택되는 아크릴산 유도체 중 적어도 하나가 15중량% 이하이고, -비공액디엔(unconjugated diene), 특히 N,N'-메틸렌-비스-아크릴아미드인교차 결합체로부터 얻어진 구성 단위의 0.1∼10중량%, 바람직하게는 0.5∼5중량%.
  2. 제1항에 있어서, 하기 비율의 모노머가 수용성 매질에서 공중합 반응으로 구성됨을 특징으로 하는 미립자의 제조방법:
    1) R1이 C1∼C4를 알킬기인 식의 아크릴릭 알데히드의 공중합유로부터 얻어지는 구성단위의 5∼95%, 바람직하게는 40∼60중량%,
    2) R2가 수소원자 및 C1∼C4를 갖는 알킬기 중에서 선택되고 R3가 저급하이드록시알킬기인 식의 아크릴산 유도체의 공중합체로부터 얻어지는 구성단위의 5∼90중량%, 바람직하게는 40∼70%,
    3) R4, R5및 R6는 같거나 또는 다른 저급알킬기 (C1∼C4)인및의 유도체 중에서 선택된 아크릴산 유도체 중 적어도 하나가 15중량% 이하이고, -비공액디엔, 특히 N,N'-메틸렌-비스-아크릴아미드인 교차결합체로 부터 얻어진 구성 단위의 0.1∼10중량%, 바람직하게는 0.5∼5중량%.
  3. 제2항에 있어서, 계면활성제는 이온 또는 비-이온성 타입이고 중합매질의 부피에 대하여 0.01∼5중량%의 양을 사용함을 특징으로 하는 방법.
  4. 제2항 또는 3항에 있어서, 수용성 중합매질에서 전체모노머 농도가 중합매질의 부피에 대해 4∼16중량%임을 특징으로 하는 방법.
  5. 제2∼4항 중 어느 한항에 따라, 공중합은 공중합 용액을 교반하고, 적어도 15분 동안 혼합물의 시간당 ㎤당 0.11∼0.5메가레드의 조사량이 사용된 고-에너지 방사능(코발트 γ선 방사능과 같은)의 영양하에서 수행됨을 특징으로 하는 방법.
  6. 제1항에 있어서, 하기중에서 선택된 항체와 액체매질에서 접촉시켜 알맞게 정제된 미립자가 얻어짐을 특징으로 하는 HBS항원의 결정을 위한 미립자의 용도:
    -정제된 HBS항원으로 면역 시킨 후 동물로부터 수득된 특이한 면역 혈청으로부터 공지된 방법에 의해 분리한 다클론성 항체 및
    -HBS항원에 대해 면역된 동물의 형질세포융합 및 적당한 특성을 갖는 골수종 세포로부터 얻어진 융합 세포(Hybridomas)를 제조한 후 공지된 방법에 의해 정제된 다클론성 항체.
  7. 제6항에 있어서, 상기 입자가 상기의 항체와 접촉한 후, 수득된 생성물을 자유 알데히드기를 보호하기 위해 과량의 1차 아민으로 처리함을 특징으로 하는 미립자의 용도.
  8. 제6항에 있어서, 상기 입자가 상기의 항체와 접촉한 후, 수득된 생성물을 수소화금속으로 처리하여 환원시킴을 특징으로 하는 미립자의 용도.
  9. 제6,7 및 8항 중 어느 한항에 따라, 생물학적 액체에서 HBS항원의 혼탁 측정 결정을 위해 처리된 미립자의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019830003808A 1982-08-16 1983-08-16 바이러스성 b형 간염 진단을 위한 시약 및 그의 제조방법 KR920000193B1 (ko)

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FR8214170A FR2531718B1 (fr) 1982-08-16 1982-08-16 Reactif permettant un dosage de tres haute sensibilite de l'antigene caracteristique du virus de l'hepatite b dans les liquides biologiques humains
FR8214170 1982-08-16

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KR920000193B1 KR920000193B1 (ko) 1992-01-10

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US (1) US4690906A (ko)
EP (1) EP0104101B1 (ko)
JP (2) JPS59108009A (ko)
KR (1) KR920000193B1 (ko)
AT (1) ATE25527T1 (ko)
CA (1) CA1228446A (ko)
DE (1) DE3369853D1 (ko)
FR (1) FR2531718B1 (ko)
GR (1) GR79632B (ko)
IL (1) IL69393A (ko)
SG (1) SG12488G (ko)

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US5141848A (en) * 1987-01-21 1992-08-25 Abbott Laboratories Confirmatory immunoassay using microparticle separation
JP2833624B2 (ja) * 1987-02-27 1998-12-09 日本化薬株式会社 診断試薬用ラテックス
US4828978A (en) * 1987-09-18 1989-05-09 Eastman Kodak Company Agglutination reagent and method of preparing same
US6326136B1 (en) * 1988-04-01 2001-12-04 Digene Corporation Macromolecular conjugate made using unsaturated aldehydes
FR2642174B1 (fr) * 1989-01-20 1994-03-25 Diagnostica Stago Particules submicroniques, utilisation dans le domaine des reactions immunologiques du type antigene
DE4014540A1 (de) * 1990-05-07 1991-11-14 Klaus Dr Tschaikowsky Immunkonjugate zur prophylaxe und therapie von organschaeden bei entzuendlichen prozessen
US6090925A (en) 1993-03-09 2000-07-18 Epic Therapeutics, Inc. Macromolecular microparticles and methods of production and use
US5981719A (en) * 1993-03-09 1999-11-09 Epic Therapeutics, Inc. Macromolecular microparticles and methods of production and use
US6316185B1 (en) * 1999-09-29 2001-11-13 Mountain View Pharmaceuticals, Inc. Quantitation of viruses by light scattering
CA3031355C (en) 2016-07-29 2023-05-23 Diazyme Laboratories, Inc. Methods and compositions for assaying vitamin d
CN110907639A (zh) * 2019-12-05 2020-03-24 四川新健康成生物股份有限公司 血清淀粉样蛋白a检测试剂盒及其制备方法

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DE2404742A1 (de) * 1974-02-01 1975-08-07 Hoechst Ag Verfahren zur herstellung von copolymeren ungesaettigter aldehyde
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US4413070A (en) * 1981-03-30 1983-11-01 California Institute Of Technology Polyacrolein microspheres
US4438239A (en) * 1981-03-30 1984-03-20 California Institute Of Technology Microsphere coated substrate containing reactive aldehyde groups

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FR2531718B1 (fr) 1986-11-21
IL69393A (en) 1987-01-30
US4690906A (en) 1987-09-01
SG12488G (en) 1989-04-21
JPH05310849A (ja) 1993-11-22
FR2531718A1 (fr) 1984-02-17
CA1228446A (en) 1987-10-20
KR920000193B1 (ko) 1992-01-10
GR79632B (ko) 1984-10-31
EP0104101A1 (fr) 1984-03-28
DE3369853D1 (en) 1987-04-02
JPH0613582B2 (ja) 1994-02-23
ATE25527T1 (de) 1987-03-15
IL69393A0 (en) 1983-11-30
JPS59108009A (ja) 1984-06-22
EP0104101B1 (fr) 1987-02-25
JPH0527064B2 (ko) 1993-04-20

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