KR830001367A - Method for manufacturing glucoamylase - Google Patents

Method for manufacturing glucoamylase Download PDF

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Publication number
KR830001367A
KR830001367A KR7904251A KR790002978A KR830001367A KR 830001367 A KR830001367 A KR 830001367A KR 7904251 A KR7904251 A KR 7904251A KR 790002978 A KR790002978 A KR 790002978A KR 830001367 A KR830001367 A KR 830001367A
Authority
KR
South Korea
Prior art keywords
glucoamylase
cellulose
immobilized
activity
duponti
Prior art date
Application number
KR7904251A
Other languages
Korean (ko)
Other versions
KR830002800B1 (en
Inventor
마사키 타무라
미즈호 시미즈
미노루 타고
Original Assignee
죤 피이 후로이드
시이피이시이 인터내쇼날 인코포레이팃드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP10635478A external-priority patent/JPS5534046A/en
Application filed by 죤 피이 후로이드, 시이피이시이 인터내쇼날 인코포레이팃드 filed Critical 죤 피이 후로이드
Publication of KR830001367A publication Critical patent/KR830001367A/en
Application granted granted Critical
Publication of KR830002800B1 publication Critical patent/KR830002800B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01G—COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSES C01D OR C01F
    • C01G23/00—Compounds of titanium
    • C01G23/04—Oxides; Hydroxides
    • C01G23/047—Titanium dioxide
    • C01G23/07—Producing by vapour phase processes, e.g. halide oxidation
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09C—TREATMENT OF INORGANIC MATERIALS, OTHER THAN FIBROUS FILLERS, TO ENHANCE THEIR PIGMENTING OR FILLING PROPERTIES ; PREPARATION OF CARBON BLACK  ; PREPARATION OF INORGANIC MATERIALS WHICH ARE NO SINGLE CHEMICAL COMPOUNDS AND WHICH ARE MAINLY USED AS PIGMENTS OR FILLERS
    • C09C1/00—Treatment of specific inorganic materials other than fibrous fillers; Preparation of carbon black
    • C09C1/36—Compounds of titanium
    • C09C1/3607—Titanium dioxide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2006/00—Physical properties of inorganic compounds
    • C01P2006/60—Optical properties, e.g. expressed in CIELAB-values
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2006/00—Physical properties of inorganic compounds
    • C01P2006/80—Compositional purity

Abstract

A glycoamylase, which retained >=90% of its activity when held at 70≰C for 10 min. at pH 4.5 and had max. activity at ~ 75≰C, was isolated from a Talaromyces duponti culture. Thus, T. duponti G 45-632 was cultured for 7 days at 40≰C in a yeast ext. starch agar medium and the glucoamylase was isolated from the culture, after filtering off the mycelia. The glucoamylase was purified by DEAE-cellulose, CM-cellulose, and sephadex G-150 chromatog. Finally, the purified glucoamylase was immobilized by binding with AE-cellulose using glutaraldehyde. Immobilized glucoamylase had 2,000 units of activity per g carrier. In a saccharification test, using a 25% soln. of starch hydrolysate at 60≰C, immobilized glucoamylase produced a saccharified soln. contg. 96.5% dextrose.

Description

열에 안정한 글루코아밀라제의 제법Preparation of heat stable glucoamylase

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 60℃에서 상대활성에 대한 pH의영향.1 shows the effect of pH on relative activity at 60 ° C.

제2도는 pH6.0에서 상대활성에 대한 온도의 영향.2 shows the effect of temperature on relative activity at pH 6.0.

제3도는 70℃에서 기질을 함유하지 않는 통상의 그로코아밀라제와 본 효소와의 열안정성의 비교.3 is a comparison of the thermal stability of a conventional grocoamylase containing no substrate at 70 ° C. with the present enzyme.

Claims (1)

탈라로마이스 듀폰티 균주 세포를 영양배지 중에서 배양하고 배양지로 부터 글루코아밀라제 효소제제를 분리하는 것으로 구성된 열에 안정한 글루코아밀라제 효소 제제의 제조방법.A method for preparing a heat stable glucoamylase enzyme preparation comprising culturing Talaomycus duponti strain cells in a nutrient medium and separating the glucoamylase enzyme preparation from the culture medium. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019790002978A 1978-09-01 1979-08-31 Preparation of heat stable glucoamylase KR830002800B1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP10635478A JPS5534046A (en) 1978-09-01 1978-09-01 Novel glucoamyrase having excellent heat resistance and production
US06/055,723 US4247637A (en) 1978-09-01 1979-07-09 Highly thermostable glucoamylase and process for its production
US055723 1979-07-09
US055,723 1979-07-09

Publications (2)

Publication Number Publication Date
KR830001367A true KR830001367A (en) 1983-04-30
KR830002800B1 KR830002800B1 (en) 1983-12-16

Family

ID=26446464

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019790002978A KR830002800B1 (en) 1978-09-01 1979-08-31 Preparation of heat stable glucoamylase

Country Status (5)

Country Link
KR (1) KR830002800B1 (en)
AU (1) AU528159B2 (en)
GB (1) GB2029835B (en)
MY (1) MY8400129A (en)
SG (1) SG6883G (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4536477A (en) * 1983-08-17 1985-08-20 Cpc International Inc. Thermostable glucoamylase and method for its production
EP0255124A3 (en) * 1986-07-29 1989-04-12 Hitachi, Ltd. Thermostable glucoamylase, a method for production of glucose using same and a plant for production thereof

Also Published As

Publication number Publication date
KR830002800B1 (en) 1983-12-16
AU4949079A (en) 1980-03-06
GB2029835A (en) 1980-03-26
AU528159B2 (en) 1983-04-14
SG6883G (en) 1984-04-19
GB2029835B (en) 1982-11-10
MY8400129A (en) 1984-12-31

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