KR810001586B1 - Degumming method of silk by fermentation - Google Patents

Abstract

Degumming of by-product silk which is raw material for spinning of silk yarn was processed by utilizing Bacillus megaterium SY-7(I), by which the degumming time, degumming degree, residual fat, tensile strength, and whiteness of silk yarn were improved. Thus, a mixt. of 1% sericin extract, small amt. of maltose, and ammonium nitrate was mixed in a fermentation broth of I and degummed at 30-35oC, pH 7.0-7.4.

Description

Refining method of raw sand by fermentation

The present invention uses Bacillus megaterium SY-7 (Bacillus megaterium SY-7) bacteria in refining silkworms as raw materials of silk-spun yarns. It is shortened, uniformly refined and has a small amount of residue. The silk or silk-shaped babe has almost complete thread glass, and the tensile The present invention relates to a method for refining silk spinning raw materials, which has advantages of being able to obtain a silk fiber having good strength and whiteness, and also after-treatment by chemical treatment, which can reduce cost and prevent sulfur change. .

Conventional refining methods of Bujasa include chemical treatment methods such as neutral soap, sodium carbonate, and surfactants, and refining methods by fermentation. However, since the former damages the fiber, the tensile strength is significantly lowered and it is easy to change to yellowish brown. Since the latter is generally performed by natural fermentation, the required refining time is long, and the skin of silk layer or raw skin is poor in glass, high residue rate and easy to be stained. There were a number of disadvantages to go through.

The present invention is to solve the above-mentioned drawbacks described in more detail as follows.

Bacillus megaterium SY-7 bacteria used in the present invention, 5 g of distilled water was added to 50 g of fresh skin (Buxamsa), boiled under 1.2 kg / cm 2 pressure for 120 minutes, and the sericin extract was filtered. Distilled water was replenished so that 5 liters of it was made into about 0.2% of sericin extract. 1 g of Beef extract, 10 g of Pepton, and 3 g of salt were added per 1 liter of the filtrate, and 1.5% of Agar in the case of a solid medium prepared at pH 6.8-7.4 was used. 30 ml each of 300 ml Erlenmeyer flasks, and put 1 g of various separation sources (fermentation tank waste liquor, various sub-silsa wastes, penny scraps, soil, gutter, etc.) and incubate at 34 ° C for 112 hours. After separation by pure water separation method, there was little damage to perbroin among them, and the highest titer of protease was selected. This bacterium was 0.6-0.8 It is about 3.0-3.8μ in size. It is active gram-positive bacteria, and the optimum growth temperature is 31-36 ℃ and pH is 6.8-7.7.

When refining Bujasa was carried out by controlling the temperature to 31-35 ℃, pH 6.5-7.5 using the Bacillus megaterium SY-7 strain (Example) is as follows.

EXAMPLE

5 liters of tap water was added to 1 kg of Bujasa, 3-4 hours of sericin extracted after treatment for 3-4 hours at 85-95 ℃, and 3g of beef and extract, 10g of peptone, and 3g of salt were added. Temperature 32-35 ℃, pH 7.0 The strain was inoculated and cultured under the conditions of -7.4, and 1% soybean meal hydrolysate and very small amounts of maltose and ammonium nitrate were added to the sub-silica refining solution, followed by inoculation culture. It refine | purified under the conditions of 30-35 degreeC, and pH 7.0-7.4.

The fermentation by spontaneous adherent bacteria under the same conditions as in Example and the fermentation refining results by the bacterium "Bacterium megaterium SY-7" of the present invention are as follows.

Note: (1) Extracted and quantified by Soxhlet apparatus.

However, 0 = anhydrous amount of sample

0 ₁ = sample anhydrous amount after oil extraction

0p = oil content

(2) Measured using Instrong's Texile Strength tester CR Type in accordance with KS-302 (Fiber Tensile Strength Test Method).

(3) Irradiated under the purple fluorescent lamp or determined by the dyeing result.

(4) Boil 2.0-2.5 hours in a solution of 7% Marseille soap, 3% sodium carbonate and 2 ml / l nonionic surfactant.

As shown in the above table, it can be seen that the refining method using Bacillus megaterium SY-7 bacteria is superior to the method of refining raw sand by fermentation by conventional common natural bacteria.

Claims (1)

As described in the text, 1% sericin extract, a small amount of maltose and ammonium nitrate were added to a culture medium of Bacillus megaterium SY-7 in fermentation method, and the temperature was 30-35 ° C., pH 7.0. Process for refining raw silk by fermentation characterized in that the refining under the conditions of -7.4.