KR800001608B1 - Process for preparing meat products - Google Patents

Process for preparing meat products Download PDF

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KR800001608B1
KR800001608B1 KR770000939A KR770000939A KR800001608B1 KR 800001608 B1 KR800001608 B1 KR 800001608B1 KR 770000939 A KR770000939 A KR 770000939A KR 770000939 A KR770000939 A KR 770000939A KR 800001608 B1 KR800001608 B1 KR 800001608B1
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gluten
powder
meat
protein
products
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가즈오 모리
요시아끼 아까바네
다까히고 안노
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모리시다 히로시
닛뽄신야구가부시기가이샤
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Abstract

Meat product contg. waeat gluten powder was prefd. by gluten modification. Thus, 150kg raw gluten was dispersed in 900kg 0.15N HC1, hydrolyzed at 1000C for 1 hr sedimented at pH 4.5, and spray dried to obtain gluten power, more easily sol. than the original material. MNeat products contg. modified gluten powder are easily soluble in water which the emulisifing property.

Description

육제품의 제조법Recipe of meat products

제1도는 발명의 상세한 설명에 기재한 실시예 1에 의해 얻어진 탈 아미드화 글루텐의 유화안정성과 와의 관계 곡선을 나타낸다.1 shows a relationship curve between the emulsion stability of the deamidated gluten obtained in Example 1 described in the detailed description of the invention.

본 발명은 개량된 글루텐(gluten)을 사용한 육제품의 제조법에 관한 것이다.The present invention relates to a method for preparing meat products using improved gluten.

근년 어업자원의 고갈, 식육, 어육원료의 고가에 의한 장차의 불안감에서 식물 단백질의 축산 및 수산연(練)제품에의 이용이 활발하게 행해지게 되었다. 식물 단백질 중에서도 특히 소맥단백질 글루텐은 높은 응집성, 부착성을 가지고, 가열했을 경우 대단히 탄력이 강한 겔을 형성하기 때문에 연제품의 탄력 보강제로서 널리 이용되어 왔다. 그러나 그 반면 글루텐은 물에 불용성의 단백질이기 때문에 흡수성이 불량하고 유화성도 적고, 겔화 온도도 높고, 제품에 다량으로 혼합했을 때에는 특유한 단단함을 보이고, 부드러운 감이 결핍된 식미(食味)로 되는 등의 결점을 가지고 있다. 이 때문에 이들의 개량에 관해서 수많은 연구를 하고 있으나 제품으로서 우수한 것이 아직 발견되지 않은 것이 현상이다. 한편 동물성 단백은 식물성 단백에 비해서 영양적으로도 우수하기는 하지만 가공원료로서의 가격유지, 공급량의 한계에서 수요에 충분히 대응하여 공급해 나가는 것이 어려웠다. 그 때문에 가격이 싼 식물성 단백에 대한 요구는 날이 갈수록 증가되어 있고 종전 이상으로 식품가공에 적합한 특성을 가진 제품의 개발이 강하게 요망되고 있었다. 그래서 본 발명자들은 이러한 현상에 비추어 소맥글루텐의 개량에 대해서 예의(銳意)연구를 한 결과 글루텐 단백질을 식품 가공에 적합하도록 개질시키는데 성공하고 개량된 글루텐을 사용해서 신규한 육제품의 제조법을 발명했다.In recent years, there has been an increase in the use of plant protein for livestock and fishery products in the future due to the depletion of fishery resources, meat and raw fish raw materials. Among the plant proteins, the wheat protein gluten has been widely used as an elastic reinforcement agent of soft products because it has high cohesiveness and adhesion, and forms a very elastic gel when heated. However, gluten is an insoluble protein in water, so it has poor absorption, low emulsification, high gelling temperature, and when mixed in a large amount, it shows peculiar hardness and lack of soft taste. It has a flaw. For this reason, a lot of research has been carried out regarding these improvements, but it is a phenomenon that excellent products have not yet been found. On the other hand, although animal protein is more nutritious than vegetable protein, it was difficult to supply enough in response to demand in terms of maintaining the price as a raw material and supply limitation. For this reason, the demand for low-cost vegetable protein has been increasing day by day, and there has been a strong demand for the development of products having characteristics suitable for food processing than before. Therefore, in light of these phenomena, the present inventors conducted a thorough study on the improvement of wheat gluten and succeeded in modifying the gluten protein to be suitable for food processing, and invented a novel meat product manufacturing method using the improved gluten.

단백질이 있는 여러가지의 식품 가공상의 기능은 일반적으로 단백질 분자내의 가전된 관능기의 분포나 양 및 그 입체구조에 유래되어 있다고 생각된다. 이것에 대해서 글루텐 단백질을 볼 때 그 구성 아미노산에 있어서 산성 아미노산인 글루텐산, 아스파라긴산이 다량으로 함유되어 있음에도 불구하고 이들의 아미노산의 대부분은 암모니아와 결합해서 소위 아미드 결합을 형성하고 있다. 그 때문에 아미드결합은 분자내 혹은 분자간에 있어서 많은 수소 결합을 형성하고 글루텐 단백질을 불용(不溶)의 단백질로 하고 있다. 그래서 본 발명자들은 이점에 착안해서 아미드 결합을 절단한 탈아미드화 글루텐을 제조한바, 단백질의 성질은 대단히 개선되고 육가공의 공정에 이용했을때 우수한 성질을 발현하는 것을 발견하고 본 발명을 완성했다.Various food processing functions with proteins are generally thought to be derived from the distribution and amount of household functional groups in protein molecules and their conformation. On the other hand, in view of the gluten protein, although the constituent amino acids contain a large amount of acidic gluten acid and aspartic acid, most of these amino acids combine with ammonia to form so-called amide bonds. Therefore, amide bonds form many hydrogen bonds in the molecule or in the molecule, and the gluten protein is made into an insoluble protein. Therefore, the present inventors made the deamidated gluten which cut | disconnected the amide bond based on this advantage, and discovered that the property of a protein is greatly improved and expresses the outstanding property when used in the process of meat processing, and completed this invention.

즉 본 발명은 염산 산성하에 분산된 글루텐을 가열 가수분해하여 글루텐 단백의 전아미드 결합의 5%-30%를 탈아미드화 시키고 그 탈아미드화된 글루텐을 건조시켜 분말로 하여 육가공품의 제조시에 첨가하는 것을 특징으로 하는 육제품의 제조법이다. 본 발명의 방법에 의해서 제조한 수산연제품 식육제품은 품질도 대단히 우수한 것이고, 본 발명의 제조방법은 우수하고 저렴한 제품 공급에 유용하다. 하기 실험예 및 실시예를 들어 본 발명을 상술한다.In other words, the present invention heat-hydrolyzes gluten dispersed under acidic hydrochloric acid to deamidize 5% -30% of all amide bonds of the gluten protein, and then dry the deamidated gluten to powder to add it in the manufacture of meat products. It is a manufacturing method of the meat product characterized by the above-mentioned. The marine fish products of the meat products produced by the method of the present invention are also of excellent quality, and the production method of the present invention is useful for supplying excellent and inexpensive products. The present invention is described in detail by the following experimental examples and examples.

[실험예 1 탈아미드화 글루텐의 유화 안정성에 대한 pH의 영향.]Experimental Example 1 Effect of pH on Emulsification Stability of Deamidated Gluten.

[실험방법]Experimental Method

글루텐 분말 50g을 0.1N염산용액에 분산시킨후 120℃에서 15분간 가열하여 가수분해한 후 용액을 냉각시키고 수산화나트륨 용액을 사용해서 pH를 4.5부근으로 조정하고 탈아미드화 글루텐을 등전침전(等電沈殿)시켜서 회수했다. 그 후 또 다시 이 침전된 탈 아미드화 글루텐을 묽은 수산화 나트륨용액에 용해하고 pH를 7.0으로 조정, 활성탄으로 처리한 후 동결 건조하여 건조분말 38g을 얻었다. 이 분말의 일부를 채취해서 각 pH로 액성을 맞춘 탈아미드화글루텐 1%수용액 혹은 1%분산액을 만들었다. 이 액 각 135㎖에 면실유 65㎖를 첨가하여 전량을 200㎖로 하고, 호모믹서를 사용해서 2분간 유화시킨 후, 유화액 10㎖를 채취해서 시험관에 넣고 30분간 방치한 후, 치험관 하층에서 유화액 5㎖를 채취해서 수분 정량을 하고 다음식에 의해서 유화 안정성을 산출한다.50 g of gluten powder was dispersed in 0.1 N hydrochloric acid solution, and then hydrolyzed by heating at 120 ° C. for 15 minutes, cooling the solution, adjusting the pH to around 4.5 using sodium hydroxide solution, and deaminated gluten isoelectric precipitation. V) to recover. Thereafter, the precipitated deamidated gluten was dissolved in dilute sodium hydroxide solution, the pH was adjusted to 7.0, treated with activated carbon, and lyophilized to obtain 38 g of a dry powder. A portion of this powder was taken to form a 1% aqueous solution of deamidated gluten or 1% dispersion adjusted to liquid pH at each pH. After adding 65 ml of cottonseed oil to each of these liquids to make 200 ml, and emulsifying it for 2 minutes using a homomixer, 10 ml of the emulsion was collected, placed in a test tube, and allowed to stand for 30 minutes. 5 ml is taken, water is quantified, and the emulsion stability is calculated by the following equation.

Figure kpo00001
Figure kpo00001

Mt : 실제로 함유되어 있던 수분 %Mt:% moisture actually contained

Mo : 본래 함유되어 있었을 수분 %Mo:% of moisture originally contained

[실험결과][Experiment result]

결과는 제1도에 표시한 대로이고 본 발명의 방법을 특징으로 하는 글루텐의 탈아미드화에 의해 개량된 단백질은 개질전의 글루텐에 비해서 식육이 지니고 있는 중성부근의 pH이고 유화성을 가지고 있으며, 유화안정성에 있어서도 우수한 것이 명백히 인정되었다.The results are as shown in FIG. 1 and the protein improved by deamidation of the gluten characterized by the method of the present invention has a pH and emulsification near the neutrality of meat compared to gluten before reforming and emulsification. It was clearly recognized that excellent also in stability.

[실험예 2 유화안정성의 탈아미드화도에 의한 영향.][Experimental Example 2 Influence by the degree of deamidation of the emulsion stability.]

[실험방법]Experimental Method

글루텐 분말 50g을 0.1N 또는 0.5N 염산용액에 분산시켜서 온도를 100℃ 또는 120℃로 조정하고 분해시간을 15분-2시간으로 변경시켜서 탈아미드화의 비율이 3%-67%로 되도록 가수분해 처리를 한 후 냉각시키고 pH를 조정한 다음 등전 침전시켜서 회수하고 또 다시 용해한 후 pH를 7.5로 조정하고 건조분말화했다. 얻어진 건조분말 시료(試料)의 1%용액을 만들고 실험예 1의 방법에 따라 유화안정성을 시험했다.Dissolve 50g gluten powder in 0.1N or 0.5N hydrochloric acid solution to adjust the temperature to 100 ℃ or 120 ℃ and change the decomposition time to 15min-2hours so that the rate of deamidation is 3% -67% After the treatment, the mixture was cooled, the pH was adjusted, then isoelectrically precipitated, recovered, dissolved again, the pH was adjusted to 7.5, and the powder was dried. A 1% solution of the obtained dry powder sample was prepared, and the emulsion stability was tested according to the method of Experimental Example 1.

[실험결과][Experiment result]

결과는 하기표에서 보인 바와같고 글루텐의 개질법으로, 탈아미드 화도가 5%-30%가 되도록 처리한 경우 유화 안정성이 우수한 것이 명백히 인정되었다. 탈아미드화도가 5%보다 작을때는 글루텐 단백질의 용해성이 약하고 유화안정성도 불량하다. 또 30%보다 커지면 유화안정성이 점차 저하되어갔다. 또 글루텐의 탈아미드화도는 2N 황산중에서 120℃, 2시간의 가수분해후 킬달(Kjeldahl)법으로 질소를 측정하여,The results are as shown in the table below, and by the method of modifying gluten, it was clearly recognized that the emulsion stability was excellent when treated to a degree of deamide degree of 5% -30%. When the degree of deamidation is less than 5%, the solubility of gluten protein is weak and the emulsion stability is poor. In addition, when it exceeds 30%, the emulsion stability gradually decreases. The degree of deamidation of gluten was measured by nitrogen using Kjeldahl method after 2 hours of hydrolysis at 120 ° C. in 2N sulfuric acid.

Figure kpo00002
Figure kpo00002

으로 나타낸다.Represented by

Figure kpo00003
Figure kpo00003

[실험예 3 어육소세지의 탄력성에 대한 영향.]Experimental Example 3 Influence on Elasticity of Fish Sausage.

[실험방법]Experimental Method

냉동명태 살을 분쇄한 것 100부를 해동시키고 잘 다져서 사이렌트 캇터중에서 세절한후 식염 3부를 첨가하고 잘 컷팅한 후 물 60부, 사탕 1부, 글루타민산 나트륨 1부 전분 8부를 첨가해서 더욱 더 잘 컷팅했다. 이 연육 100부에 실험예 2에서 얻어진 건조분말 각각 2부를 첨가하여 잘 혼합한 후 라아드(돼지기름) 20부를 첨가하고 더욱 잘 혼합시킨 후 케이싱에 채우고 80℃의 열탕중에서 30분간 가열한 후 냉각하여 어육소세지를 만들었다. 이 어육소세지를 15㎜두께로 절단하고 텍스츄로메터에 의해 탄력성을 측정했다.100 parts of frozen pollack meat is thawed, chopped, and chopped in a siren cutter to add 3 parts of salt, and then cut well. did. Add 2 parts of dry powder obtained in Experiment 2 to 100 parts of the meat, mix well, add 20 parts of laad (pig), mix well, fill the casing, heat for 30 minutes in a hot water bath at 80 ° C, and then cool. Fish meat sausage. This fish sausage was cut to a thickness of 15 mm and the elasticity was measured by a texturometer.

[실험결과][Experiment result]

다음 결과표에서 보인 것과 같고 글루텐의 개질법에 의해 얻어진 분말을 첨가해서 만든 어육 소세지 탄력성 내지 ''족(足)''(Hardness)가 대조구(區)와 비교해서 더욱 좋고 라아드(돼지기름)의 유화가 잘 되며 식미도 부드러운 것으로 되었다. 어육소세지에 있어서의 유화성도 실험예 2와 같은 경향을 보이고 탈아미드화도 30%이상에서는 어육소세지의 품질은 명백히 저하되었다. 또 탈아미드화도 3%의 검체도 유화가 잘되지 않고 어육소세지의 탄력성도 불량했다.As shown in the following result table, fish meat sausage elasticity or `` hardness '' made by adding the powder obtained by the reforming method of gluten is better than the control, and emulsification of Laard It is well and the taste is soft. The emulsification in fish sausage also showed the same tendency as Experimental Example 2, and the quality of fish sausage was clearly deteriorated at a deamidation degree of 30% or more. In addition, the deamidation and the 3% of the specimens did not emulsify well, and the meat sausage had poor elasticity.

Figure kpo00004
Figure kpo00004

[실시예 1]Example 1

소맥분에서 분리된 생글루텐 150㎏을 0.15N염산용액 900㎏중에 교반하면서 분산시키고 100℃에서 한시간 동안 가열 가수분해를 한 후 냉각하고 pH를 4.5로 조정하고 탈아미드화 글루텐을 등전 침전시켜서 회수한다. 회수후 0.01N수산화나트륨용액 800㎏에 용해하고 pH를 7.5로 조정후 여별(濾別)해서 불용물을 제거한다. 다음에 이 여액을 탈색, 탈취 처리한 후 스프레이드라이어에 의해 개량된 글루텐 건조분말 33㎏을 얻었다. 이 분말의 탈아미드화도는 15%이었다. 이 분말 2.5㎏을 5℃이하로 보유한 양육과 돈육의 혼합물 100㎏에 25㎏의 물을 첨가하여 잘 혼합된 것에 섞고 잘 혼합시킨다. 그 후 식염 3㎏, 설탕 0.5㎏ 글루타민산나트륨 0.4㎏, 인산염 0.6㎏을 첨가해서 잘 교반 혼합한다. 그 후 교반 혼합한 것을 냉장고 2일동안 방치한다. 2일간 침전시킨후 통상 방법에 의해 컷팅을 하고 적당량의 전분, 향신료를 첨가해서 소세지를 제조한다.150 kg of raw gluten separated from wheat flour is dispersed in 900 kg of 0.15 N hydrochloric acid solution with stirring, heated by hydrolysis at 100 ° C. for one hour, cooled, adjusted to pH 4.5, and recovered by isostatic precipitation of deamidated gluten. After recovery, the solution was dissolved in 800 kg of 0.01 N sodium hydroxide solution, the pH was adjusted to 7.5, and the mixture was filtered to remove insoluble matters. Next, the filtrate was decolorized and deodorized, and 33 kg of a gluten-dried powder improved by a spray dryer was obtained. The deamidation degree of this powder was 15%. 2.5 kg of this powder is added to 100 kg of the mixture of rearing and pork which is kept below 5 ° C., 25 kg of water is mixed and mixed well. Thereafter, 3 kg of salt, 0.5 kg of sugar, 0.4 kg of sodium glutamate, and 0.6 kg of phosphate are added, followed by stirring and mixing well. After that, the mixture was stirred and left for 2 days in the refrigerator. After settling for 2 days, cutting is carried out by the usual method, and sausage is prepared by adding an appropriate amount of starch and spices.

[실시예 2]Example 2

세단(細斷)시킨 신선한 대구 100부를 5-10배량의 물로 5회 세척하여 바랜후 6회째의 물로 바랠때에 식염을 첨가해서 농도를 0.2%정도로 하고 이것을 압착 탈수하여 탈수어육 70부를 얻었다. 이 탈수육 100부를 간 후 인산염(폴리인산 나트륨) 0.2부 설탕 3부 및 실시예 1에서 얻은 개량 글루텐 10부를 첨가해서 30분간 연마하여 점조성이 강한 육호(肉糊)를 얻었다. 이것을 깊이 5㎝의 아연 도금한 철판제 용기에 넣어서 25℃에서 약 3시간을 보낸 후 동결시켜 냉동 분쇄육을 제조한다.100 parts of fresh cod was washed 5 times with 5-10 times of water, and when the water was changed to the 6th water, salt was added to make the concentration about 0.2%, and this was compressed and dehydrated to obtain 70 parts of dehydrated fish meat. After 100 parts of this dehydrated meat, 0.2 parts of phosphate (sodium polyphosphate) and 3 parts of sugar and 10 parts of the improved gluten obtained in Example 1 were added and ground for 30 minutes to obtain a viscous consistency. This was placed in a galvanized iron plate container having a depth of 5 cm, and then frozen for about 3 hours at 25 ° C to prepare frozen ground meat.

Claims (1)

0.1N-0.5N염산에 분산시킨 글루텐을 100℃-120℃에서 15분-2시간 가열 가수분해시켜 글루텐 단백의 전아미드 결합의 5%-30%를 탈아미드화하고 이것을 건조시켜 분말로 해서 육가공품의 제조시에 첨가하는 것을 특징으로 하는 육제품의 제조법.Gluten dispersed in 0.1N-0.5N hydrochloric acid was heated and hydrolyzed at 100 ° C-120 ° C for 15 minutes-2 hours to deamidize 5% -30% of all amide bonds of the gluten protein, dried to powder A method for producing a meat product, which is added at the time of manufacture of a product.
KR770000939A 1977-04-19 1977-04-19 Process for preparing meat products KR800001608B1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100972933B1 (en) * 2007-12-12 2010-07-28 샘표식품 주식회사 Sausage containing wheat gluten hydrolysate and method for producing the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100972933B1 (en) * 2007-12-12 2010-07-28 샘표식품 주식회사 Sausage containing wheat gluten hydrolysate and method for producing the same

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