KR20250051161A - 클래스 ii, 유형 ii crispr 시스템 - Google Patents

클래스 ii, 유형 ii crispr 시스템 Download PDF

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Publication number
KR20250051161A
KR20250051161A KR1020257011372A KR20257011372A KR20250051161A KR 20250051161 A KR20250051161 A KR 20250051161A KR 1020257011372 A KR1020257011372 A KR 1020257011372A KR 20257011372 A KR20257011372 A KR 20257011372A KR 20250051161 A KR20250051161 A KR 20250051161A
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lys
arg
endonuclease
glu
leu
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Korean (ko)
Inventor
브라이언 토마스
크리스토퍼 브라운
오드라 디보토
크리스티나 버터필드
리사 알렉산더
다니엘라 에스 에이 골츠맨
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메타지노미, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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KR1020257011372A 2020-03-31 2021-03-30 클래스 ii, 유형 ii crispr 시스템 Pending KR20250051161A (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US202063003159P 2020-03-31 2020-03-31
US63/003,159 2020-03-31
US202063116149P 2020-11-19 2020-11-19
US63/116,149 2020-11-19
PCT/US2021/024945 WO2021202568A1 (en) 2020-03-31 2021-03-30 Class ii, type ii crispr systems
KR1020227037087A KR102794727B1 (ko) 2020-03-31 2021-03-30 클래스 ii, 유형 ii crispr 시스템

Related Parent Applications (1)

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KR1020227037087A Division KR102794727B1 (ko) 2020-03-31 2021-03-30 클래스 ii, 유형 ii crispr 시스템

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KR20250051161A true KR20250051161A (ko) 2025-04-16

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KR1020257011372A Pending KR20250051161A (ko) 2020-03-31 2021-03-30 클래스 ii, 유형 ii crispr 시스템
KR1020227037087A Active KR102794727B1 (ko) 2020-03-31 2021-03-30 클래스 ii, 유형 ii crispr 시스템

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US (2) US11946039B2 (enExample)
EP (2) EP4632066A3 (enExample)
JP (2) JP7546689B2 (enExample)
KR (2) KR20250051161A (enExample)
CN (1) CN116096877B (enExample)
AU (1) AU2021248800A1 (enExample)
CA (1) CA3177051A1 (enExample)
ES (1) ES3055163T3 (enExample)
GB (1) GB2608292B (enExample)
MX (1) MX2022012110A (enExample)
WO (1) WO2021202568A1 (enExample)

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CA3177051A1 (en) 2020-03-31 2021-10-07 Metagenomi, Inc. Class ii, type ii crispr systems
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MX2024005505A (es) 2021-11-05 2024-05-23 Metagenomi Inc Enzimas de edicion de bases.
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US11946039B2 (en) 2024-04-02
AU2021248800A1 (en) 2022-10-13
WO2021202568A1 (en) 2021-10-07
GB2608292A (en) 2022-12-28
JP7546689B2 (ja) 2024-09-06
US20240309356A1 (en) 2024-09-19
CN116096877A (zh) 2023-05-09
EP4127156A4 (en) 2024-03-27
JP7785139B2 (ja) 2025-12-12
CA3177051A1 (en) 2021-10-07
KR102794727B1 (ko) 2025-04-11
EP4127156C0 (en) 2025-09-10
JP2024178181A (ja) 2024-12-24
EP4632066A3 (en) 2025-12-24
KR20220161383A (ko) 2022-12-06
CN116096877B (zh) 2026-04-28
MX2022012110A (es) 2022-10-18
GB202211839D0 (en) 2022-09-28
US20230051396A1 (en) 2023-02-16
EP4632066A2 (en) 2025-10-15
ES3055163T3 (en) 2026-02-10
EP4127156B1 (en) 2025-09-10
JP2023519953A (ja) 2023-05-15
GB2608292B (en) 2024-02-14
EP4127156A1 (en) 2023-02-08

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