KR20240139088A - 킬로베이스 규모의 rna 가이드 게놈 리콤비니어링 - Google Patents

킬로베이스 규모의 rna 가이드 게놈 리콤비니어링 Download PDF

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Publication number
KR20240139088A
KR20240139088A KR1020247030192A KR20247030192A KR20240139088A KR 20240139088 A KR20240139088 A KR 20240139088A KR 1020247030192 A KR1020247030192 A KR 1020247030192A KR 20247030192 A KR20247030192 A KR 20247030192A KR 20240139088 A KR20240139088 A KR 20240139088A
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protein
cell
recombinant protein
sequence
aptamer
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KR1020247030192A
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English (en)
Korean (ko)
Inventor
리 콩
쳉쿤 왕
유안하오 쿠
Original Assignee
더 보드 오브 트러스티스 오브 더 리랜드 스탠포드 주니어 유니버시티
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Priority claimed from PCT/US2022/075850 external-priority patent/WO2023034925A1/en
Application filed by 더 보드 오브 트러스티스 오브 더 리랜드 스탠포드 주니어 유니버시티 filed Critical 더 보드 오브 트러스티스 오브 더 리랜드 스탠포드 주니어 유니버시티
Publication of KR20240139088A publication Critical patent/KR20240139088A/ko
Pending legal-status Critical Current

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C12N9/226—Class 2 CAS enzyme complex, e.g. single CAS protein
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    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Medicinal Chemistry (AREA)
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  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020247030192A 2022-02-10 2023-02-10 킬로베이스 규모의 rna 가이드 게놈 리콤비니어링 Pending KR20240139088A (ko)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US202263308834P 2022-02-10 2022-02-10
US202263308837P 2022-02-10 2022-02-10
US202263308830P 2022-02-10 2022-02-10
US63/308,837 2022-02-10
US63/308,834 2022-02-10
US63/308,830 2022-02-10
USPCT/US2022/075850 2022-09-01
PCT/US2022/075850 WO2023034925A1 (en) 2021-09-01 2022-09-01 Rna-guided genome recombineering at kilobase scale
PCT/US2023/062406 WO2023154877A2 (en) 2022-02-10 2023-02-10 Rna-guided genome recombineering at kilobase scale

Publications (1)

Publication Number Publication Date
KR20240139088A true KR20240139088A (ko) 2024-09-20

Family

ID=87565146

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KR1020247030192A Pending KR20240139088A (ko) 2022-02-10 2023-02-10 킬로베이스 규모의 rna 가이드 게놈 리콤비니어링

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Country Link
US (1) US20250354164A1 (https=)
EP (1) EP4476333A4 (https=)
JP (1) JP2025505732A (https=)
KR (1) KR20240139088A (https=)
CN (1) CN119855904A (https=)
AU (1) AU2023217087A1 (https=)
CA (1) CA3249564A1 (https=)
IL (1) IL314544A (https=)
WO (1) WO2023154877A2 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4396340A4 (en) * 2021-09-01 2025-09-24 Univ Leland Stanford Junior RNA-GUIDED GENOME RECOMBINATION AT THE KILOBASE SCALE
WO2024168265A1 (en) * 2023-02-10 2024-08-15 Possible Medicines Llc Aav delivery of rna guided recombination system
CN117247858B (zh) * 2023-07-06 2024-07-23 云南农业大学 一种消减烟草连作障碍并提质增产的菌剂及其制法和应用
WO2026010985A1 (en) * 2024-07-02 2026-01-08 The General Hospital Corporation Regeneration of biological tissues

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017106657A1 (en) * 2015-12-18 2017-06-22 The Broad Institute Inc. Novel crispr enzymes and systems
WO2018081535A2 (en) * 2016-10-28 2018-05-03 Massachusetts Institute Of Technology Dynamic genome engineering
EP3728604A2 (en) * 2017-12-22 2020-10-28 KWS SAAT SE & Co. KGaA Targeted transcriptional regulation using synthetic transcription factors
IL296057A (en) * 2020-03-03 2022-10-01 Univ Leland Stanford Junior Ribonucleic acid-guided genome recombination engineering at the scale of thousands of bases
WO2023154892A1 (en) * 2022-02-10 2023-08-17 Possible Medicines Llc Rna-guided genome recombineering at kilobase scale

Also Published As

Publication number Publication date
US20250354164A1 (en) 2025-11-20
CA3249564A1 (en) 2023-08-17
EP4476333A4 (en) 2026-02-25
CN119855904A (zh) 2025-04-18
WO2023154877A3 (en) 2023-09-14
IL314544A (en) 2024-09-01
AU2023217087A1 (en) 2024-08-22
EP4476333A2 (en) 2024-12-18
JP2025505732A (ja) 2025-02-28
WO2023154877A2 (en) 2023-08-17

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