AU2023217087A1 - Rna-guided genome recombineering at kilobase scale - Google Patents

Rna-guided genome recombineering at kilobase scale Download PDF

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Publication number
AU2023217087A1
AU2023217087A1 AU2023217087A AU2023217087A AU2023217087A1 AU 2023217087 A1 AU2023217087 A1 AU 2023217087A1 AU 2023217087 A AU2023217087 A AU 2023217087A AU 2023217087 A AU2023217087 A AU 2023217087A AU 2023217087 A1 AU2023217087 A1 AU 2023217087A1
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Prior art keywords
protein
composition
cell
recombination
sequence
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AU2023217087A
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English (en)
Inventor
Le Cong
Yuanhao QU
Chengkun WANG
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Leland Stanford Junior University
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Leland Stanford Junior University
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Priority claimed from PCT/US2022/075850 external-priority patent/WO2023034925A1/en
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of AU2023217087A1 publication Critical patent/AU2023217087A1/en
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
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    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2023217087A 2022-02-10 2023-02-10 Rna-guided genome recombineering at kilobase scale Pending AU2023217087A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US202263308834P 2022-02-10 2022-02-10
US202263308837P 2022-02-10 2022-02-10
US202263308830P 2022-02-10 2022-02-10
US63/308,837 2022-02-10
US63/308,834 2022-02-10
US63/308,830 2022-02-10
AUPCT/US2022/075850 2022-09-01
PCT/US2022/075850 WO2023034925A1 (en) 2021-09-01 2022-09-01 Rna-guided genome recombineering at kilobase scale
PCT/US2023/062406 WO2023154877A2 (en) 2022-02-10 2023-02-10 Rna-guided genome recombineering at kilobase scale

Publications (1)

Publication Number Publication Date
AU2023217087A1 true AU2023217087A1 (en) 2024-08-22

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ID=87565146

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AU2023217087A Pending AU2023217087A1 (en) 2022-02-10 2023-02-10 Rna-guided genome recombineering at kilobase scale

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US (1) US20250354164A1 (https=)
EP (1) EP4476333A4 (https=)
JP (1) JP2025505732A (https=)
KR (1) KR20240139088A (https=)
CN (1) CN119855904A (https=)
AU (1) AU2023217087A1 (https=)
CA (1) CA3249564A1 (https=)
IL (1) IL314544A (https=)
WO (1) WO2023154877A2 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4396340A4 (en) * 2021-09-01 2025-09-24 Univ Leland Stanford Junior RNA-GUIDED GENOME RECOMBINATION AT THE KILOBASE SCALE
WO2024168265A1 (en) * 2023-02-10 2024-08-15 Possible Medicines Llc Aav delivery of rna guided recombination system
CN117247858B (zh) * 2023-07-06 2024-07-23 云南农业大学 一种消减烟草连作障碍并提质增产的菌剂及其制法和应用
WO2026010985A1 (en) * 2024-07-02 2026-01-08 The General Hospital Corporation Regeneration of biological tissues

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017106657A1 (en) * 2015-12-18 2017-06-22 The Broad Institute Inc. Novel crispr enzymes and systems
WO2018081535A2 (en) * 2016-10-28 2018-05-03 Massachusetts Institute Of Technology Dynamic genome engineering
EP3728604A2 (en) * 2017-12-22 2020-10-28 KWS SAAT SE & Co. KGaA Targeted transcriptional regulation using synthetic transcription factors
IL296057A (en) * 2020-03-03 2022-10-01 Univ Leland Stanford Junior Ribonucleic acid-guided genome recombination engineering at the scale of thousands of bases
WO2023154892A1 (en) * 2022-02-10 2023-08-17 Possible Medicines Llc Rna-guided genome recombineering at kilobase scale

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US20250354164A1 (en) 2025-11-20
CA3249564A1 (en) 2023-08-17
EP4476333A4 (en) 2026-02-25
CN119855904A (zh) 2025-04-18
WO2023154877A3 (en) 2023-09-14
IL314544A (en) 2024-09-01
KR20240139088A (ko) 2024-09-20
EP4476333A2 (en) 2024-12-18
JP2025505732A (ja) 2025-02-28
WO2023154877A2 (en) 2023-08-17

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