KR20240137862A - Cosmetic for preventing and treating acnes containing Genistein - Google Patents

Abstract

The present invention relates to a cosmetic for preventing and treating acne, and the cosmetic according to the present invention is characterized by containing genistein as an effective ingredient. The cosmetic containing genistein can be used safely without any side effects on the skin, and is excellent in controlling sebum secretion by inhibiting the activity of 5 alpha-reductase and in antibacterial and anti-inflammatory effects, so it is effective in preventing and treating acne.

Description

{Cosmetic for preventing and treating acne containing Genistein}

The present invention relates to a cosmetic for preventing and treating acne, and more specifically, to a cosmetic which is not only safe to use without side effects on the skin, but also excellent in preventing and treating acne by containing genistein, which has excellent antibacterial and anti-inflammatory effects and controls sebum secretion by inhibiting the activity of 5 alpha-reductase.

Acne is a chronic inflammatory disease of the sebaceous glands that occurs mainly on the face, chest, and neck of young men and women, such as those in their puberty years, and forms comedones, papules, pustules, cysts, or nodules, and in severe cases, can leave scars. If the skin damage caused by acne is severe, it can cause psychological damage such as depression or avoidance of interpersonal relationships.

The cause of acne has not yet been clearly identified, but if genetic and environmental factors are excluded, it is believed to be caused by the following three factors.

First, there is the case where sebum is secreted abnormally in excess. Excessive sebum secretion is closely related to the male hormone testosterone, and it is known that testosterone is converted into dihydrotestosterone (DHT) by 5 alpha-reductase in skin cells, which increases the activity of sebum-secreting cells and promotes sebum secretion (Rosenfield RL and Deplewski D. Role of androgen in the developmental biology of the pilosebaceous unit. The American Journal of Medicine 1995;16:80s-88s).

Second, when the hair follicle wall is hyperkeratinized and the pore is blocked. The sebum that is produced should be discharged smoothly out of the skin, but when the pore is blocked due to hyperkeratinization of the hair follicle, the smooth discharge of sebum is inhibited, so sebum stagnates in the hair follicle and forms micro-comedones. This condition is the initial stage of acne.

Third, stagnant sebum in the hair follicle promotes the growth of Propionibacterium acnes, an anaerobic bacterium that resides in the hair follicle, thereby worsening acne lesions. As acne bacteria proliferate in the sebaceous glands, the triglycerides that make up the sebum are converted into free fatty acids, which act as irritants or comedogenic substances, promoting acne production and resulting in an inflammatory response (Sergio Nacht, Cosmetics Toiletries, 101, 47-55, 1986; Wei-Li Lee et al., Infection and Immunity, 35, 71-78, 1982).

Accordingly, the current evaluation methods for acne treatment and relief effects are also based on the aforementioned mechanism of action, including methods for evaluating the effects of controlling sebum secretion, suppressing hyperkeratosis, antibacterial activity against acne bacteria, and alleviating inflammation caused by acne bacteria (Madli Puhvel, Cosmetics Toiletries, 98, 117-119, 1983; Kathy Perisho et al., J. of Invest. Dermatol., 90, 350-353, 1988; Hyeyoung Kim et al., Korean J. Ginseng Sci., 14, 391-398, 1990; R.A. Bojar et al., British J. of Dermatology, 132, 204-208, 1995; A. M. Kligman et al., British J. of Dermatology, 100, 699-702, 1979).

Acne treatment methods using medication can be largely categorized into topical application therapy and oral therapy. If acne symptoms are mild, topical application therapy alone can be sufficiently treated. Among topical antibiotics used as medicines, clindarmycin is known to be the most effective, and combination drugs that mix erythromycin and tetracycline are mainly used.

Also, as a commonly used method, acne is treated by suppressing the formation of dead skin cells in pores or removing them to promote secretion by using preparations containing salicylic acid or vitamin A derivatives such as retinoic acid, or by removing dead skin cells and alleviating inflammation through antibacterial action by using preparations containing benzoyl peroxide, triclosan, sulfur or tea tree oil.

However, salicylic acid preparations not only have minimal therapeutic effects, but can also cause skin redness, swelling, or skin dermatitis, and retinoic acid preparations are effective in suppressing hyperkeratosis, but can cause contact dermatitis, erythema, dry skin, and peeling. In addition, benzoyl peroxide and trichlorosan preparations have side effects such as allergic contact dermatitis, scarring, and severe erythema, so caution is required when using them. On the other hand, azelaic acid preparations, which were introduced relatively recently, have reduced drug side effects, but their therapeutic effects are inferior to those of existing treatment products.

Therefore, the technical problem to be achieved by the present invention is to provide a cosmetic that can be safely used without side effects on the skin by solving the above problems, and is capable of controlling sebum secretion by inhibiting the activity of 5 alpha-reductase, and has excellent antibacterial and anti-inflammatory effects, thereby being excellent in preventing and treating acne.

Composition and operation of the invention

In order to achieve the above purpose, the cosmetic for preventing and treating acne according to the present invention is characterized by containing genistein as an effective ingredient.

In the cosmetic according to the present invention, the content of genistein is preferably 0.001 to 10 wt% based on the total weight of the cosmetic.

The cosmetic according to the present invention may further contain an anti-keratosis agent such as salicylic acid, retinoic acid, a retinoic acid derivative, or benzoyl peroxide, and the cosmetic may be manufactured into various formulations such as skin, lotion, cream, foundation, essence, gel, pack, foam cleansing, or soap.

Hereinafter, a cosmetic for preventing and treating acne according to the present invention will be described in detail.

Genistein is a type of isoflavone and its structural formula is as follows.

Genistein is a compound classified as an isoflavone, phytoestrogen, and polyphenol. It is found in many products such as soybeans, potatoes, or beans. It is known to reduce adipose tissue, inhibit cell differentiation of adipocytes, and prevent the process of fat inheritance.

It also lowers cholesterol and triglyceride levels. It has a muscle-protecting effect that prevents muscle atrophy and damage, and it also affects the growth of muscle mass. It reduces the concentration of cortisol, commonly called the stress hormone, which has a negative effect on fat metabolism and bones. It also supports glucose transport and regulates carbohydrate metabolism, reducing insulin resistance.

According to the results of various clinical trials described below, genistein not only causes little irritation to the skin, unlike conventional acne treatments, but also inhibits the activity of 5-alpha-reductase to reduce sebum secretion, inhibits the growth of Propionibacterium acnes, and relieves inflammation, thereby showing great effectiveness in preventing and treating acne.

According to the present invention, when genistein is added to cosmetics such as skin, lotion, cream, foundation, essence, and gel, in addition to the basic cosmetic function, it can simultaneously achieve the effect of preventing and treating acne.

In addition, genistein can be applied in various ways to products that require cleansing, such as packs, foam cleansing, and soap. In particular, if substances that can suppress or remove hyperkeratosis, such as salicylic acid, retinoic acid, retinoic acid derivatives, and benzoyl peroxide, are added to cosmetics, acne can be prevented and treated more effectively.

In the cosmetic composition for preventing and treating acne of the present invention, the content of genistein is preferably 0.001 to 10 wt% based on the total weight of the cosmetic composition. If the content of genistein is less than 0.001 wt%, the acne treatment effect may be lower than expected, and if it exceeds 10 wt%, the mixing of the cosmetic composition may not be easy. In this respect, it is more preferable to add 0.001 to 2 wt% of genistein.

Hereinafter, in order to specifically explain the present invention, experimental examples and examples will be described in detail. However, the examples according to the present invention can be modified in various different forms, and the scope of the present invention should not be construed as being limited to the examples described below. The examples of the present invention are provided in order to more completely explain the present invention to a person having average knowledge in the art.

The cosmetic for preventing and treating acne containing genistein of the present invention can be safely used without side effects on the skin, and is excellent in controlling sebum secretion by inhibiting the activity of 5 alpha-reductase and in antibacterial and anti-inflammatory effects, so it is very useful for preventing and treating acne.

Figure 1 - Evaluation test for sebum secretion inhibition effect
Figure 2 - Sebum secretion suppression effect
Figure 3 - Evaluation of anti-inflammatory effect
Figure 4 - Primary irritation test on human skin (closed patch test)
Figure 5 - Primary irritation test on human skin (closed patch test)
Figure 6 - Sebum secretion speed evaluation test
Figure 7 - Evaluation test for inhibition of cotton formation
Figure 8 - Comparison table of cotton area
Figure 9 - Cotton wadding effect evaluation test

Genistein's sebum secretion inhibitory effect

Experimental Example 1

The following experiment was conducted to investigate the inhibition of genistein's 5 alpha-reductase activity and confirm its effect of inhibiting sebum secretion.

In 30 ㎕ of phosphate buffered saline (PBS / pH 6.6), 10 ㎕ of testosterone (3H-1,2,4,5,6,7-testosterone) bound to dithiothreitol, NADPH, and radioactive isotope, 40 ㎕ of 5 alpha-reductase enzyme extract, and 20 ㎕ of 0.001% genistein were mixed, respectively. Then, after reacting at room temperature for 10 minutes, the amount of converted dihydrotestosterone was measured using a combination of HPLC and a radioisotope meter, and the inhibition rate (inhibition rate (%)) of genistein against 5 alpha-reductase enzyme was calculated according to the following formula, and the results are shown in Table 1 below.

Inhibition rate (%) = (Conversion rate of testosterone to dihydrotestosterone in the sample without genistein added) × 100 / (Conversion rate of testosterone to dihydrotestosterone in the sample with genistein added)

Experimental examples 2-4

The experiment was conducted in the same manner as Experimental Example 1, except that the concentration of genistein was changed as described in Table 1.

Genistein concentration (%) Inhibition rate (%) Experimental Example 1 0.001 20.10 Experimental example 2 0.010 33.00 Experimental Example 3 0.100 42.80 Experimental example 4 1.000 100.00

As shown in Table 1 above, genistein is found to be very effective in inhibiting the activity of 5 alpha-reductase.

Evaluation of the antibacterial activity of genistein against Propionibacterium acnes

Experimental Example 5

To measure the antibacterial activity of genistein against Propionibacterium acnes (hereinafter referred to as “P. acnes”), P. acnes that had been stored in a refrigerated state in a bacteriostatic state were cultured on Brain Heart Infusion (BHI) solid plate medium 3 days prior to the start of the experiment, and then the pre-cultured P. acnes was inoculated onto BHI solid plate medium containing a certain concentration of genistein on the day of the experiment. Then, the number of P. acnes after 3 days was measured to obtain the minimum inhibitory concentration (MIC), which is shown in Table 2.

Example 1

The experiment was conducted in the same manner as Experimental Example 5, except that benzoyl peroxide was used instead of genistein.

Sample MIC(Mole) Experimental Example 5 Genistein 110~600ppm Example 1 Benzoyl peroxide 550~1,100ppm

Referring to Table 2 above, it can be seen that genistein has a better P. acnes growth inhibition effect than benzoyl peroxide, which is used as an effective ingredient in conventional acne treatment agents.

Evaluation of the anti-inflammatory effect of genistein

Experimental example 6

When inflammation is formed by acnes, acne becomes worse. The anti-inflammatory effect of genistein was evaluated to confirm whether genistein has an acne-alleviating effect. The experimental method was as follows: The evaluation method using arachidonic acid (A. Crummey, G. P. Harper, E. A. Boyle and F. R. Mangan. Inhibition of arachidonic acid-induced ear oedema as a model for assessing topical anti-inflammatory compound. Agents and Actions 1987;20:69-76) was used.

After cleaning both ears of the mouse with ethanol before sample application, 20 ㎕ of a 0.3% concentration genistein sample was applied to the sample application group, and 20 ㎕ of ethanol was applied to the control group once a day for 4 days continuously. Finally, 1 hour after the application, ethanol was applied to the left ear, and 2 mg/ear of arachidonic acid was applied to the right ear. 1 hour after application, the degree of ear edema was repeatedly measured 3 times for each ear using a micrometer. The left ear of the treatment group was used as a control group to determine the degree of inflammation induced by the sample itself.

The anti-inflammatory effect was determined by the degree of swelling inhibition in the genistein sample application group based on the arachidonic acid treatment control group, and the results are shown in Table 3 below. The number of mice used in each treatment group was 7, and the measurement value is the average value for 7 mice.

Contrast examples 2-3

Instead of genistein samples, stearyl glycyrrhetinate and indomethacin were used respectively.

The experiment was conducted in the same manner as Experimental Example 6, except that (indomethacin) was used.

Treatment group Sample density(%) Solvent Ear thickness (㎛) Inhibition rate (%) Before sample processing After sample processing Control group Arachidonic acid 2mg/ear Ethanol 270 490 - Experimental example 6 Genistein 0.3 Ethanol 282 440 26.8 Example 2 Stearyl Glycyrrhetinate 0.3 Ethanol 278 464 13.8 Example 3 Indomethacin 0.3 Ethanol 293 401 22.6

※ Suppression rate (%) = (A―B) / A × 100

A: Change in average thickness of the control ear (thickness of the ear treated with arachidonic acid - thickness of the ear not treated)

B: Change in average thickness of the sample-applied group's ears (thickness of sample-treated ears - thickness of untreated ears)

As shown in Table 3, the genistein sample showed a high inflammation inhibition rate of 26.8%, which is higher than that of stearyl glycyrrhetinate in Comparative Example 2, which is known to have a good anti-inflammatory effect, and was shown to have the same anti-inflammatory effect as indomethacin in Comparative Example 3, which is a substance with an excellent anti-inflammatory effect as a pharmaceutical ingredient. In addition, when verifying the significance difference with the edema increase rate, genistein had a meaningful effect with arachidonic acid at a significance level of 99%.

From these experimental examples, it can be seen that genistein has excellent inhibitory effects on 5 alpha-reductase activity, antibacterial effects on P. acnes, and anti-inflammatory effects.

Below, the efficacy of adding genistein to cosmetics such as gels, lotions, essences, and creams for suppressing sebum secretion, alleviating acne, and improving acne in vivo was evaluated. Experiments were also conducted on other skins, foundations, and packs, and similar effects were obtained, but are omitted here.

Example 1 and Comparative Example 1

A gel was prepared with the ingredients and contents as shown in Table 4 below.

Raw material name Example 1 (weight%) Comparative Example 1 (weight%) Glycerin 3.00 3.00 Dipropylene glycol 5.00 5.00 Carboxyvinyl polymer 0.20 0.20 Ethanol 10.00 10.00 Polyoxyrene-cured castor oil 0.30 0.30 Triethanolamine 0.20 0.20 Genistein 0.10 - antiseptic dose dose spices dose dose pigment dose dose Purified water TO 100 TO 100

Example 2 and Comparative Example 2

A lotion was prepared with the ingredients and contents as shown in Table 5 below.

Raw material name Example 2 (weight%) Comparative Example 2 (weight%) Cholesteryl ester 2.00 2.00 Cetostearyl alcohol 0.80 0.80 Stearic acid 1.00 1.00 Squalane 8.00 8.00 Refined Jojoba Oil 4.00 4.00 Octyldodecanol 2.00 2.00 Polysorbate 1.00 1.00 Sorbitan sesquiolenic acid 0.50 0.50 glycerin 5.00 5.00 Carboxyvinyl polymer 0.20 0.20 Triethanolamine 0.30 0.30 Genistein 0.50 - antiseptic dose dose spices dose dose pigment dose dose Purified water to 100 to 100

Example 3 and Comparative Example 3

An essence was prepared with the ingredients and contents as shown in Table 6 below.

Raw material name Example 3 (weight%) Comparative Example 3 (weight%) Ethanol 6.00 6.00 Glycerin 5.00 5.00 polyacrylic acid 0.20 0.20 1,3-Butylene glycol 10.00 10.00 Hydroxyethyl cellulose 0.10 0.10 Carboxyethylene cellulose 0.10 0.10 Triethanolamine 0.20 0.20 Ethylene oxide 0.30 0.30 Genistein 0.30 - antiseptic dose dose Fragrance dose dose pigment dose dose Purified water to 100 to 100

Example 4 and Comparative Example 4

A cream was prepared with the ingredients and contents as shown in Table 7 below.

Raw material name Example 4 (weight%) Comparative Example 4 (weight%) Microstalinic acid 0.50 0.50 Cetostearyl alcohol 3.00 3.00 Stearic acid 2.00 2.00 Squalane 5.00 5.00 Stearic acid glycerin 2.00 2.00 Octyldodecanol 3.00 3.00 Polysorbate 1.50 1.50 Sorbitan sesquiolenic acid 0.50 0.50 Liquid paraffin 8.00 8.00 Octyldodecyl myristate 4.00 4.00 glycerin 6.00 6.00 Dipropylene glycol 5.00 5.00 Genistein 0.50 - Preservative dose dose spices dose dose pigment dose dose Purified water to 100 to 100

Primary irritation test on human skin (closed patch test)

In order to determine whether the cosmetics according to the above examples are harmful to the human body, a first irritation test on human skin was conducted using a human patch test as follows.

The experiment was conducted on 50 healthy adult men and women according to the CTFA guidelines (The Cosmetic, Toiletry and Fragrance Association. Inc. Washington, D.C., 20036, 1991). 20㎕ of cosmetics according to the examples and comparative examples were dropped into a Finn Chamber, placed on the test area, the back of the human body, and fixed with tape. Then, after 24 hours of application, the patch was removed, and after another 4 hours, the skin reaction of the test area was visually read according to the following criteria, and the results are shown in Table 8 below.

<Judging criteria>

- : No erythema or unusual phenomenon

+- ; Slightly redder than the surrounding area

+ ; Significantly redder than surrounding area

++; Swells and becomes redder than the surrounding area

Stimulus = [{(+-)number × 1} + {(+)number × 2} + {(++)number × 3}]/number of subjects

Sample Number of test subjects Verdict Stimulus ++ + +- - Example 1 50 0 0 0 50 0.00 Comparative Example 1 50 0 0 3 47 0.06 Example 2 50 0 0 0 50 0.00 Comparative Example 2 50 0 0 2 48 0.04 Example 3 50 0 0 0 50 0.00 Comparative Example 3 50 0 0 1 49 0.02 Example 4 50 0 0 0 50 0.00 Comparative Example 4 50 0 0 2 48 0.04

As shown in Table 8 above, the cosmetics of Examples 1 to 4 containing genistein showed lower irritation than the cosmetics of Comparative Examples 1 to 4 not containing genistein. This is thought to be due to the excellent anti-inflammatory effect of genistein, as shown in the anti-inflammatory effect experiment described above. Therefore, it was confirmed that genistein is a very safe substance for the skin when applied to cosmetic formulations.

Sebum secretion inhibition evaluation test

To evaluate the sebum secretion inhibition effect, 40 healthy adult men and women were randomly selected and the sebum secretion inhibition effect using sebum tape (sebutape CuDerm Corp., TX, USA) was observed. The experimental method is as follows.

First, the normal sebum secretion rate in the forehead area to be used in the experiment was measured using sebum tape, and then Examples 1 to 3 and Comparative Examples 1 to 3 were applied for 3 weeks, and then the sebum secretion rate was measured using sebum tape in the same area where the normal sebum secretion rate was measured. The sebum tape was attached for 30 minutes and then removed, and the amount of sebum secreted on the sebum tape was measured using an image analyzer, and the determination was made using the sebum secretion rate, and the results are shown in Table 9 below. The unit of the sebum secretion rate is ㎍/㎠/min, which means the amount of sebum secreted per unit time and per unit area.

Sample Sebum secretion rate (㎍/㎠/min) (Comparative Example-Example)/Comparative Example × 100 Example 1 0.92 33.33% Comparative Example 1 1.38 Example 2 0.89 38.62% Comparative Example 2 1.45 Example 3 0.82 46.05% Comparative Example 3 1.52

Referring to Table 9 above, it can be seen that the samples of Examples 1 to 3 according to the present invention have a superior effect on reducing sebum secretion speed compared to the samples of Comparative Examples 1 to 3. Accordingly, the effect on reducing the amount of sebum secreted is also further enhanced. As shown in the experimental examples described above, it is believed that this is due to the inhibition of 5 alpha-reductase activity by genistein.

Evaluation of the effect of inhibiting the formation of cotton using rabbit ears

The anti-comedone formation effect was evaluated using a rabbit ear experiment method, which is widely known as an acne efficacy evaluation method (A.M.Kligman et al., British Journal of Dermatology, 100, 699-702, 1979). This comedone formation experiment is somewhat different from acne formation in humans, but it is currently the closest experimental method to creating a condition similar to the pre-inflammatory stage of acne by creating comedones.

First, 0.5 ml of the samples of Examples 1 to 3 were applied to one ear of a rabbit, and 0.5 ml of the samples of Comparative Examples 1 to 3 were applied to the other ear of a rabbit, and then 0.5 ml of isopropyl myristate was applied to each ear after 5 hours. This treatment was repeated every day for 2 weeks, and the effect of inhibiting comedones formation was evaluated through visual judgment at 7 levels of '0', '0.5', '1', '1.5', '2', '2.5', and '3' according to the following criteria. In addition, a whole mount was created from a rabbit ear, and the area of the comedones (based on square millimeter) was measured and evaluated using an image analyzer. The results are shown in Tables 10 and 11 below, respectively. Seven rabbits were used in each experimental example.

<Visual judgment criteria>

0.0: No difference from the control group

0.5: Symptoms between '0' and '1'

1.0: Congestion, capillary hemorrhage, mild keratosis pilaris and hypertrophy of the pores can be seen.

1.5: Symptoms between '1' and '2'

2.0: Moderate keratosis pilaris and hypertrophy of pores

2.5: Symptoms between '2' and '3'

3.0: Severe keratosis and pore enlargement, typical comedones observed

Sample Visual judgment (Comparative Example-Example)/Comparative Example × 100 Example 1 1.50 42.75% Comparative Example 1 2.62 Example 2 1.61 44.86% Comparative Example 2 2.92 Example 3 1.61 43.11% Comparative Example 3 2.83

Sample Comparison of cotton area (Comparative Example-Example)/Comparative Example × 100 Example 1 1.250 43.28% Comparative Example 1 2.204 Example 2 1.112 56.04% Comparative Example 2 2.530 Example 3 1.231 49.80% Comparative Example 3 2.452

Referring to the above results, in the comparison of the acne area by visual judgment or using an image analyzer, the samples of Examples 1 to 3 are expected to be more effective in inhibiting acne formation than the samples of Comparative Examples 1 to 3, and thus are expected to be very effective in preventing acne.

Evaluation of cotton pad relaxation effect using rabbit ears

The anti-comedogenic effect of genistein was evaluated using the rabbit ear anti-comedogenic effect evaluation method described above.

First, 0.5㎖ of isopropyl myristate was applied to each rabbit ear for 2 weeks to induce cotton swabs, and then 0.5㎖ each of the samples of Examples 1 to 3 were applied to one ear and 0.5㎖ each of the samples of Comparative Examples 1 to 3 were applied to the other ear for 2 weeks. After application for 2 weeks, the cotton swab relief effect was evaluated through visual judgment by dividing it into 7 stages of '0', '0.5', '1', '1.5', '2', '2.5', and '3' according to the following criteria. In addition, a whole mount was created from the rabbit ear, and the cotton swab area (based on ㎟) was measured and evaluated using an image analyzer. The results are shown in Tables 12 and 13 below. Seven rabbits were used in each experimental example.

<Visual judgment criteria>

0.0: No difference from the control group

0.5: Symptoms between '0' and '1'

1.0: Congestion, capillary hemorrhage, mild keratosis pilaris and hypertrophy of the pores can be seen.

1.5: Symptoms between '1' and '2'

2.0: Moderate keratosis pilaris and hypertrophy of pores

2.5: Symptoms between '2' and '3'

3.0: Severe keratosis and pore enlargement, typical comedones observed

Sample Visual judgment (Comparative Example-Example)/Comparative Example × 100 Example 1 1.00 44.75% Comparative Example 1 1.81 Example 2 1.00 45.36% Comparative Example 2 1.83 Example 3 1.01 46.56% Comparative Example 3 1.89

Sample Comparison of cotton area (Comparative Example-Example)/Comparative Example × 100 Example 1 1.043 37.62% Comparative Example 1 1.672 Example 2 1.008 39.17% Comparative Example 2 1.663 Example 3 1.053 38.96% Comparative Example 3 1.725

Referring to the above results, it can be seen that the samples of Examples 1 to 3 have a superior cotton relaxation effect than the samples of Comparative Examples 1 to 3 in comparison of cotton area by visual judgment or by using an image analyzer.

The above-mentioned effects of suppressing and alleviating acne formation are considered to be the comprehensive results of the sebum secretion suppression, inflammation alleviation, and antibacterial effects against acne of genistein, which have been demonstrated in the aforementioned experimental examples. Through the experiments on suppressing and alleviating acne formation using the ears of rabbits, it was found that the samples of Examples 1 to 3 according to the present invention have a remarkable effect on preventing and alleviating acne. Accordingly, the following experiments were conducted to determine whether acne alleviation effects are present in actual clinical practice.

Acne relief effect evaluation

In order to examine the acne alleviation effect of the cosmetic according to the present invention, a blind test was conducted on 120 healthy adult men and women aged 20 to 30 who had acne.

120 men and women were randomly divided into six groups of 20 each, and the samples of Examples 1 to 3 and Comparative Examples 1 to 3 were placed in the same container. Each group was instructed to use Examples 1 to 3 and Comparative Examples 1 to 3 twice a day for two months. The evaluation was conducted by comprehensively combining the subjects' questionnaires on the aggravation and alleviation of acne symptoms at two-week intervals and the visual findings of two dermatologists, and the evaluation was completed at the final two-month mark. The results are shown in Table 14 below.

Sample It's effective (noun) Slightly effective (noun) I don't know (noun) No effect (noun) Example 1 13/20 6/20 1/20 01/20 Comparative Example 1 00/20 3/20 3/20 14/20 Example 2 12/20 8/20 0/20 00/20 Comparative Example 2 00/20 3/20 5/20 15/20 Example 3 15/20 5/20 0/20 00/20 Comparative Example 3 00/20 3/20 1/20 16/20

Referring to Table 14 above, the samples of Examples 1 to 3 had 'effective' and 'slightly effective' acne alleviation effects at 90 to 100%, while the comparative examples 1 to 3, which did not contain the effective genistein ingredient at all, had effects at about 10 to 15%.

Therefore, it can be seen that the cosmetic according to the present invention shows an excellent acne alleviation effect.

Not applicable

Claims (5)

A cosmetic for preventing and treating acne characterized by containing genistein as an active ingredient.
In claim 1, a cosmetic for preventing and treating acne characterized in that the content of genistein is 0.001 to 10 wt% based on the total weight of the cosmetic
A cosmetic for preventing and treating acne, characterized in that it further contains a hyperkeratosis inhibitor in claim 1.
A cosmetic for preventing and treating acne, characterized in that in claim 1, the hyperkeratosis inhibitor comprises at least one selected from the group consisting of salicylic acid, retinoic acid, retinoic acid derivatives, and benzoyl peroxide.
A cosmetic for preventing and treating acne, characterized in that in claim 1, the cosmetic is formed into any one formulation selected from the group consisting of skin, lotion, cream, foundation, essence, gel, pack, foam cleansing, and soap.