KR20240092673A - automatic sample analysis apparatus - Google Patents

Abstract

An automatic sample analysis device is disclosed. The automatic sample analysis device of the present invention includes a chip magazine in which a plurality of electrochemical chips, each having a reaction unit that reacts with a detection target contained in a liquid sample, are mounted; and a sample supply unit that suctions a set amount of the liquid sample from the sample storage unit where the liquid sample is stored and then automatically transfers the liquid sample to the plurality of reaction units and sequentially dispenses the liquid sample. According to the present invention, storage of the liquid sample, suction of the liquid sample, supply of the liquid sample to the top of the electrochemical chip, discharge of the remaining unreacted liquid sample, detection and analysis of the actual detection target substance (phthalate) using a probe, etc. A series of analysis tasks can be performed entirely automatically, which not only improves the reliability of measurement results but also allows a large amount of analysis work to be performed quickly.

Description

Automatic sample analysis apparatus}

The present invention relates to an automatic sample analysis device that can automatically determine and analyze whether a liquid sample contains a substance to be detected.

In general, water quality testing examines the physical properties of water, chemical components contained in water, microorganisms, etc., when selecting a water source, checking and monitoring contamination or purification work of the water source, and checking tap water against water quality standards. It is carried out for a variety of purposes, such as when testing for acceptance or failure, when measuring the environmental pollution level of the sea, lake, river or stream, when determining the suitability of well water for drinking at public health centers, etc., or when inspecting the pollution level of industrial water supply or sewage. .

In order to test water quality, it is necessary to collect samples on site that can be used as a standard for overall water quality. If the object of water quality test is deep water such as the sea, lake, river, or stream, water quality varies depending on the water depth. In order to increase the accuracy, samples should be collected by water depth or samples with average water quality should be collected at a standard water depth. In this case, a water sampler that can collect samples from deep above the water is used.

The water collected in the water sampler is transported to a test room, etc., and then the tester moves the water contained in the water sampler into a separate small container and manually supplies it in the form of droplets to the surface of the test chip through a dropper, etc. to measure the target substance in the water. It is analyzed whether it is included and, if so, at what concentration/ratio.

However, in the past, the above-described work of analyzing water collected in a water sampler was all done manually, so there was a problem that the analysis results could be different depending on the tester. For example, water must be supplied in a fixed amount to the test chip, but there was a problem in that the fixed quantity supply was difficult to apply uniformly depending on the tester.

In addition, since this series of tasks are performed manually, there is an inevitable problem that the time required for water quality analysis increases and the analysis cost increases.

Republic of Korea Patent Publication No. 10-1252284 (registered on April 2, 2013)

The present invention was developed to solve the above-described conventional problems, including storage of liquid samples, suction of liquid samples, supply of liquid samples to the top of the electrochemical chip, discharge of the remaining unreacted liquid samples, and practical detection using a probe. The purpose is to provide an automatic sample analysis device that can automatically perform a series of analysis tasks such as detection and analysis of target substances (phthalates).

According to one aspect of the present invention, a chip magazine in which a plurality of electrochemical chips are seated, each having a reaction unit that reacts with a detection target contained in a liquid sample; and a sample supply unit that suctions a set amount of liquid samples from a sample storage unit where the liquid samples are stored and then automatically transfers the liquid samples onto the plurality of reaction units and sequentially dispenses the sample. An automatic sample analysis device is provided.

a voltage application unit having a probe to apply voltage by sequentially contacting electrodes of a plurality of electrochemical chips seated in the chip magazine; And it may further include a determination unit that determines whether the liquid sample contains the detection target substance by detecting current changes in the plurality of electrochemical chips to which a set voltage is applied through the voltage application unit.

It may further include a magazine tilting unit for tilting the chip magazine to remove remaining liquid samples that do not react with the detection target substance from the chip magazine.

A tilting bracket is integrally provided on one side of the chip magazine, and the magazine tilting unit includes a drive motor; and a cam connected to the rotation shaft of the drive motor and periodically contacting the tilting bracket as the drive motor rotates to push the tilting bracket upward.

The magazine tilting part not only supports the chip magazine from below, but also allows one end of the chip magazine to be hinged and rotatable, and has an inner bottom surface for discharging the remaining liquid sample that does not react with the detection target substance to the outside. It may include a magazine base provided with a drain hole.

The chip magazine is provided with a plurality of chip seating grooves so that the plurality of electrochemical chips are respectively seated, and on one side of the plurality of chip seating grooves, the chip magazine is tilted and communicates with the plurality of chip seating grooves, respectively. A plurality of sample discharge holes may be provided so that the remaining liquid sample that does not react with the detection target material is discharged.

A plurality of discharge portions each having a communication hole may be protruded from the bottom of the chip magazine to communicate with the plurality of sample discharge holes.

The sample supply unit includes a sample storage unit having a plurality of compartmentalized accommodation spaces in which the liquid sample is stored; A pipette storage unit in which a plurality of pipettes are stored; and a pipette fixing pin for gripping the pipette, and after gripping any one of the plurality of pipettes stored in the pipette storage unit and transferring it to the sample storage unit, the liquid sample from the sample storage unit is sucked. It may include a sample transfer unit that repeatedly performs the operation of transferring and dispensing onto the reaction unit.

The sample supply unit further includes a washing liquid storage unit in which washing liquid is stored, and the sample transfer unit dispenses the liquid sample onto the reaction unit, separates the pipette currently being gripped, and grips a new pipette, It may be driven to suction the washing liquid for washing foreign substances not attached to the surface of the reaction unit in addition to the detection target substance and dispense it onto the plurality of reaction units.

The sample transfer unit may be configured to reciprocate the pipette fixing pin for gripping the pipette in the x, y, and z axes.

The voltage application unit may be configured to reciprocate the probe in the x, y, and z axes.

It may further include an air fan for removing the remaining liquid sample that remains on the top of the electrochemical chip on the chip magazine and does not react with the detection target material from the chip magazine by blowing it to the outside of the chip magazine.

According to the automatic sample analysis device of the present invention described above, storage of the liquid sample, suction of the liquid sample, supply of the liquid sample to the top of the electrochemical chip, discharge of the remaining unreacted liquid sample, and actual detection target material using a probe ( A series of analysis tasks such as phthalate (Phthalate) detection and analysis can be performed entirely automatically, which not only improves the reliability of measurement results but also allows a large amount of analysis work to be carried out quickly.

In addition, by applying a structure in which the chip magazine rotates tilting, liquid samples that do not react with the reaction part of the electrochemical chip can be automatically removed, thereby increasing analysis efficiency.

In addition, by applying a structure in which the chip magazine rotates tilting, in the process of the washing liquid flowing down to the sample discharge hole along with the unreacted liquid sample, the washing liquid washes away foreign substances other than phthalate that may attach to the reaction part. Accordingly, only phthalate may remain attached to the reaction unit, and ultimately, the determination unit can perform phthalate detection/analysis work more efficiently, thereby improving the reliability of the analysis results.

1 is a one-way perspective view showing an automatic sample analysis device according to an embodiment of the present invention;
Figure 2 is a perspective view in the other direction of Figure 1;
Figure 3 is a front view of Figure 1;
Figure 4 is a perspective view showing area IV of Figure 3;
Figure 5 is a plan view of Figure 1;
Figure 6 is a perspective view showing area VI of Figure 5;
Figure 7 is a perspective view showing the chip magazine and magazine tilting portion of the automatic sample analysis device according to an embodiment of the present invention;
Figure 8 is a perspective view showing the chip magazine in a tilted state in Figure 7;
Figure 9 is a bottom perspective view of the chip magazine of the automatic sample analysis device according to an embodiment of the present invention;
Figures 10 and 11 are diagrams showing the operation sequence of the automatic sample analysis device according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in more detail with reference to the attached drawings. However, the present invention is not limited to the embodiments disclosed below and will be implemented in various different forms. These embodiments only serve to ensure that the disclosure of the present invention is complete and to fully convey the scope of the invention to those skilled in the art. This is provided to inform you. In the drawings, like symbols refer to like elements.

The sample analysis device according to a preferred embodiment of the present invention analyzes a liquid sample to detect detection target substances contained in the liquid sample. Specifically, the sample analysis device includes water collected from rivers, streams, reservoirs, oil supply pipes, water pipes, etc. Phthalate can be detected, storing liquid samples, inhaling liquid samples, supplying liquid samples to the top of the electrochemical chip, discharging the remaining unreacted liquid samples, and actually detecting the target substance (phthalate) using a probe. and analysis tasks can be performed entirely automatically.

Hereinafter, the present invention will be described in detail with reference to examples.

As shown in Figures 1 to 5, the sample analysis device according to an embodiment of the present invention includes a chip magazine 100, a sample supply unit 200, a voltage application unit 300, and a determination unit 500.

First, as shown in FIGS. 1 to 5, the chip magazine 100 includes a plurality of electrochemical chips 10 each having a reaction unit 11 that reacts with the target substance to be detected contained in the liquid sample. It is made to settle down.

Specifically, the chip magazine 100 is provided with a plurality of chip seating grooves 110 so that the plurality of electrochemical chips 10 are respectively seated, and has an overall square plate structure. The electrochemical chip 10 has a reaction part 11 coated on the surface to react with a detection target substance, and this reaction part 11 can be applied as a screen printed carbon electrode.

In an embodiment of the present invention, as shown in FIGS. 6 to 9, a tilting bracket 120 is integrally provided on one side of the chip magazine 100, and the tilting bracket 120 is periodically and repeatedly pushed to move the chip magazine. Tilting rotation of (100) can be performed, which will be described later.

In an embodiment of the present invention, on one side of the plurality of chip seating grooves 110, the remaining liquid sample that does not react with the detection target material is discharged from the chip magazine 100 that is tilted and in communication with the plurality of chip seating grooves 110, respectively. Preferably, a plurality of sample discharge holes 130 are provided.

To elaborate, a reaction unit 11 is provided on the surface of the electrochemical chip 10 so that the detection target substance (phthalate) is attached to the surface of the electrochemical chip by reacting with the set detection target substance to be detected. Except for phthalate, the remaining liquid samples need to be removed from the top of the electrochemical chip.

To this end, as shown in FIGS. 6 to 9, the present invention includes a magazine tilting unit 600 for tilting the chip magazine 100 to remove the remaining liquid sample that does not react with the detection target substance from the chip magazine 100. ) includes.

Here, as shown in FIGS. 7 and 8, the magazine tilting unit 600 is connected to the drive motor 610 and the rotation axis of the drive motor 610 and is tilted according to the rotation of the drive motor 610. It includes a cam 620 that periodically contacts the 120 to push the tilting bracket 120 upward. As the cam 620 rotates and periodically pushes the tilting bracket 120, the chip magazine 100 may also periodically repeat tilting rotation.

To elaborate, as described later, the liquid sample is dispensed (automatically dropped) onto the reaction unit 11 and the washing liquid (buffer) is similarly dispensed onto the reaction unit 11, and then the drive motor 610 is rotated. As the chip magazine 100 is tilted and rotated upward, the remaining liquid sample and washing liquid (buffer), excluding the detection target substances attached to the reaction unit 11, react with the reaction unit 11. It may break away from the phase and be removed, that is, it may flow down.

In this way, the remaining liquid sample and washing liquid that do not react with the detection target material in the chip magazine 100 fall downward to the chip magazine 100 through the sample discharge hole 130.

In an embodiment of the present invention, as shown in FIGS. 7 and 8, the magazine tilting unit 600 not only supports the chip magazine 100 from below, but also allows one end of the chip magazine 100 to hinge and rotate. Includes a magazine base 630 that is coupled.

The magazine base 630 contacts the bottom of the chip magazine 100 and supports the chip magazine from below when the chip magazine 100 is not rotated upward by the cam 620.

As shown in FIG. 8, a drain hole 640 is provided on the inner bottom surface of the magazine base 630 for discharging the washing liquid to the outside along with the remaining liquid samples that do not react with the detection target substance. The liquid sample and washing liquid discharged through the drain hole 640 are collected separately and then discarded.

Therefore, when the chip magazine 100 tilts and rotates upward, the liquid sample on the electrochemical chip 10 and the washing liquid described later flow down along the upper surface and then toward the magazine base 630 through the sample discharge hole 130. It falls.

On the other hand, it is desirable to allow the liquid sample and washing liquid discharged through the sample discharge hole 130 to be stably guided to the drain hole 640. If there is no such guiding structure, it bounces around during the falling process and into the surrounding work space. Problems that may cause contamination may occur.

To solve this problem, as shown in FIG. 9, a plurality of discharge portions 140 each having a communication hole 141 to communicate with a plurality of sample discharge holes 130 are protruded from the bottom of the chip magazine 100. It is desirable to be Therefore, the distance between the location where the unreacted liquid sample and the washing liquid are finally separated from the chip magazine 100 and the inner bottom surface of the magazine base 630 is further reduced, so that they do not splash to the surroundings but onto the magazine base 630. It can be induced stably.

Meanwhile, the drain hole 640 is formed in the central portion of the magazine base 630, and the liquid sample and washing liquid that fall toward the edge area of the inner bottom surface of the magazine base 630 are efficiently drained through the central drain hole 640. As shown in FIG. 8, the inner bottom surface of the magazine base 630 is preferably formed to be inclined entirely downward toward the drain hole 640. In addition, the bottom surface of the magazine base 630 is preferably inclined in all directions around the drain hole 640. Accordingly, liquid samples and washing liquid that have fallen to any location on the bottom surface of the magazine base 630 can be efficiently guided to the drain hole 640 and quickly discharged.

Next, as shown in FIGS. 1 to 5, the sample supply unit 200 sucks a set amount of liquid sample from the sample storage unit 210 where the liquid sample is stored, and then inhales the liquid sample from the plurality of electrochemical chips 10. It is automatically transferred to the reaction unit 11 and supplied through a sequential dispensing process.

Specifically, the sample supply unit 200 includes a sample storage unit 210, a pipette storage unit 220, a washing liquid storage unit 230, and a sample transfer unit 240.

The sample storage unit 210 has a plurality of compartmentalized accommodation spaces where liquid samples are stored. For example, 12 storage spaces are provided in the sample storage unit 210, and in each space, liquid samples collected from reservoirs, oil supply pipes, oil pipelines, etc. are separated and accommodated at 2-hour intervals based on 24 hours.

In addition, liquid samples (water) collected from reservoirs, oil supply pipes, oil pipelines, etc. are collected at intervals of 2 hours in a separate water sampler (not shown), stored separately, and then pumped as shown in Figures 1 to 5. They are individually accommodated in each receiving space of the sample storage unit 210 by 250 and the multi-valve 260. Therefore, liquid samples collected at different times can be stored in the 12 spaces of the sample storage unit 210, and rather than receiving liquid samples in all 12 spaces at the same time, the liquid samples are stored sequentially over time. Acceptance is achieved, and this operation can be achieved through operation control of the pump 250 and the multi-valve 260.

The pipette storage unit 220 provides a space in which a plurality of pipettes 221 are stored, and the plurality of pipettes 221 are placed in the pipette storage unit 220 so that one end with a relatively expanded opening faces upward. They are stored in an aligned state with set intervals.

The washing liquid storage unit 230 provides a space where the washing liquid is stored, and the washing liquid is a buffer solution that is used in liquid samples other than the detection target substance (phthalate) attached to the surface of the reaction unit 11 of the electrochemical chip 10. By washing foreign substances that are included but do not adhere to the surface of the reaction unit, sensing sensitivity can be improved so that the determination unit 500, which will be described later, can more efficiently and accurately identify and analyze phthalate.

In this way, foreign substances that may attach to the surface of the reaction unit 11 are washed by dispensing a liquid sample onto the reaction unit 11 of the chip magazine 100 and then dispensing washing liquid on the chip, as described above. This is done in the process of tilting and rotating the magazine 100 by driving the cam 620 to flow the unreacted liquid sample and washing liquid downward.

That is, in the process of the washing liquid flowing down toward the sample discharge hole 130 along with the unreacted liquid sample, the washing liquid removes foreign substances not attached to the reaction unit 11 other than phthalate that may attach to the reaction unit 11. It is washed away, and as a result, only phthalate may remain attached to the reaction unit 11. Ultimately, the determination unit 500 can perform phthalate detection/analysis more efficiently and improve the reliability of the analysis results. there is.

Next, as shown in FIGS. 1 to 5, the sample transport unit 240 is provided with a pipette fixing pin unit 241 for gripping the pipette 221, and includes a plurality of pipettes stored in the pipette storage unit 220. After gripping any one of the pipettes 221 and transferring it to the sample storage unit 210, the liquid sample in the sample storage unit 210 is sucked and transferred onto the reaction unit 11 to be dispensed. It is done so that

In addition, the sample transfer unit 240 dispenses the liquid sample onto the reaction unit 11, separates the pipette 221 that is currently gripping, and grips a new pipette 221, and then, in addition to the detection target substance, the reaction unit 11 (11) The operation of suctioning washing liquid for washing foreign substances that may adhere to the surface and dispensing it onto a plurality of reaction units (11) is performed repeatedly.

Hereinafter, the operational relationship of the automatic sample analysis device according to an embodiment of the present invention will be described with reference to FIGS. 10 and 11.

Describing the operation process, as shown in FIG. 10, the sample transfer unit 240 transfers the pipette fixing pin unit 241 to the pipette storage unit 220 and attaches the pipette fixing pin unit 241 to one of the pipettes 221. ) and then grip the pipette (221). Here, the operation of gripping the pipette 221 on the pipette fixing pin portion 241 may use various methods such as separate mechanical gripping and vacuum adsorption.

Thereafter, the sample transfer unit 240 transfers the pipette 221 to the sample storage unit 210, inserts the pipette 221 into the sample storage unit 210, and sucks a fixed amount of the liquid sample into the pipette 221. For this purpose, as shown in FIGS. 1 to 5, the sample supply unit 200 includes a syringe (not shown) for suctioning the liquid sample by creating suction pressure in the inner space of the pipette 221, and a syringe (not shown). ) may form suction pressure while moving in the up and down direction by the syringe driving unit 242, or may move to the initial position for forming suction pressure.

Here, the syringe driving unit 242 may have a ball screw structure capable of reciprocating the syringe (not shown) in the up and down direction. In addition, a gear rack, cylinder, etc. capable of reciprocating the syringe in a straight line may be applied.

Thereafter, the sample transfer unit 240 transfers the pipette 221 through which the liquid sample has been sucked to the upper side of the reaction unit 11 of the electrochemical chip 10 seated on the chip magazine 100, and the syringe driver 242 A fixed amount of liquid sample is dispensed onto the reaction unit 11 through driving.

Here, a fixed amount of liquid sample can be supplied at all times by driving the syringe driver 242 to move the syringe (not shown) back and forth a set distance.

Thereafter, the sample transfer unit 240 transfers the pipette 221 to the outside, separates the pipette 221 currently gripped by the pipette fixing pin unit 241, and drops it into a separate waste collection space.

Afterwards, it goes through an incubation process. Incubation is a delay time process that provides sufficient reaction time so that the target substance to be detected, that is, phthalate, reacts stably with the reaction unit 11 of the electrochemical chip. The phthalate reacted with the reaction unit 11 is reacted with the reaction unit (11). 11) It remains attached (remnant) to the surface.

Thereafter, the sample transfer unit 240 transfers the pipette fixing pin unit 241 to the pipette storage unit 220, inserts the pipette fixing pin unit 241 into one of the pipettes 221, and then grips the pipette 221. do.

Thereafter, the sample transfer unit 240 transfers the pipette 221 to the washing liquid storage unit 230, inserts the pipette 221 into the washing liquid storage unit 230, and then connects the syringe driver 242 and the syringe (not shown) to the washing liquid storage unit 230. ) is driven to inhale a fixed amount of washing liquid into the pipette 221.

Thereafter, the sample transfer unit 240 transfers the pipette 221, into which the washing liquid has been sucked, to the upper side of the reaction unit 11 of the electrochemical chip 10 seated on the chip magazine 100, and the syringe driver 242. A fixed amount of washing liquid is dispensed onto the reaction unit 11 through driving. At this time, the liquid sample has already been supplied to the reaction unit 11 through the above-described process.

Thereafter, the sample transfer unit 240 transfers the pipette 221 to the outside, separates the pipette 221 currently gripped by the pipette fixing pin unit 241, and drops it into a separate waste collection space.

Thereafter, as shown in FIG. 11, the magazine tilting unit 600 is driven to tilt and rotate the chip magazine 100 upward.

At this time, the detection target substance reacting with the reaction unit 11 remains attached to the reaction unit 11 and does not escape from the reaction unit 11 even when tilting and rotating. In contrast, unreacted liquid samples containing components that do not react with the reaction unit 11 flow downward when the chip magazine 100 is tilted and rotated and fall toward the magazine base 630 through the sample discharge hole 130.

In addition, the washing liquid flows downward when the chip magazine 100 is tilted and rotated, washing away foreign substances not attached to the reaction unit 11, except for phthalate attached to the reaction unit 11, and foreign substances other than phthalate are washed away. falls through the sample discharge hole along with the washing liquid.

Accordingly, only phthalate may be attached and remain on the reaction unit 11, and ultimately, the determination unit 500 can perform phthalate detection/analysis work more efficiently and improve the reliability of the analysis results.

In an embodiment of the present invention, the process of supplying washing liquid to the top of the electrochemical chip and the tipping process of the chip magazine can be repeated at least twice, and therefore, as described above, only phthalate is further attached to the reaction unit 11. By leaving it in a residual state, the determination unit 500 can more efficiently perform phthalate detection/analysis work and improve the reliability of the analysis results.

Next, the voltage application unit 300 automatically moves the probe 310 to contact the electrode 12 of the electrochemical chip 10 and applies a set voltage. Thereafter, the determination unit 500 detects changes in current of the electrochemical chip 10 to determine and analyze the concentration of the detection target substance in the liquid sample.

In an embodiment of the present invention, as shown in FIGS. 1 to 5, the sample transfer unit 240 reciprocates the pipette fixing pin unit 241 for gripping the pipette 221 in the x, y, and z axes. To this end, the pipette fixing pin part moving part 245 reciprocates the pipette fixing pin part 241 in the x, y, and z axes, and a suction pressure is formed in the internal space of the pipette 221 to collect the liquid sample. It includes a syringe driver 242 for driving a syringe (not shown) for suction.

The pipette fixing pin moving part 245 is an It includes a y-direction pipette fixing pin moving part 247 that reciprocates along the direction, and a z-direction pipette fixing pin moving part 248 that reciprocates the pipette fixing pin moving part 245 along the z-direction, which is the vertical direction, Each pipette fixing pin moving part can be applied as a ball screw unit.

However, it is not limited to this, and each pipette fixing pin moving part can be changed in various ways, such as a gear-rack unit or cylinder unit that can reciprocate the pipette fixing pin in the x, y, and z directions.

In an embodiment of the present invention, as shown in FIGS. 1 to 6, the voltage application unit 300 sequentially contacts the electrodes 12 of the plurality of electrochemical chips 10 mounted on the chip magazine 100. It includes a probe 310 capable of applying voltage, and a probe moving unit 320 that reciprocates the probe 310 in the x, y, and z axes.

The probe 310 is fixed to the probe fixing plate 311, and the probe moving unit 320 moves the probe 310 to the upper side of the electrode 12 of the electrochemical chip 10 and then lowers it to the electrode 12. Contact the upper surface of . Afterwards, the voltage application unit 300 applies the set applied voltage to the electrode 12 through the probe 310.

For example, a voltage can be applied between 0.2V and -0.2V, and if an oxidation reaction occurs between the detection target material and the reaction unit 11, the voltage can be applied by sequentially changing the voltage from -0.2V to 0.2V. Conversely, if a reduction reaction occurs between the detection target material and the reaction unit 11, the voltage can be applied while sequentially changing from 0.2V to -0.2V.

As shown in Figures 1 to 5, the probe moving unit 320 includes an A y-direction probe moving unit 322 that reciprocates the probe fixing plate 311 along the left-right y-direction, and a z-direction probe moving unit 323 that reciprocates the probe fixing plate 311 along the up-and-down z-direction. ), and each probe moving part can be applied as a ball screw unit.

However, it is not limited to this, and each probe moving unit can be changed in various ways, such as a gear-rack unit or a cylinder unit that can reciprocate the probe fixing plate 311 in the x, y, and z directions, respectively.

Next, as shown in FIG. 1, the determination unit 500 detects the change in current of the electrodes 12 of the plurality of electrochemical chips 10 to which a set voltage is applied through the voltage application unit 300 and Determine and analyze whether the detection target substance is included, that is, whether phthalate is included, and at what concentration value phthalate is included.

Specifically, the determination unit 500 detects a change in the current value and compares the current value peak point with the set reference value. If the measured peak current value is more than the set reference value, it determines that phthalate is contained more than the reference value, and the measured peak current value is higher than the set reference value. If the current value is less than the set standard value, it is judged that phthalate is contained less than the standard value.

The automatic sample analysis device according to an embodiment of the present invention has been described above, and the present invention includes tilting the chip magazine 100 to remove the remaining liquid sample that does not react with the detection target substance from the chip magazine 100. In addition to the unit 600, other modified structures may be employed.

Specifically, the automatic sample analysis device according to another embodiment of the present invention collects the remaining liquid sample that remains on the top of the electrochemical chip 10 on the chip magazine 100 and does not react with the detection target substance to the outside of the chip magazine 100. It may further include an air fan (not shown) for removal from the chip magazine 100 by blowing.

When replacing the magazine tilting unit 600 with an air fan (not shown), the operation related to magazine tilting can be omitted in the above-described operation process.

Although the present invention has been shown and described in connection with preferred embodiments for illustrating the principles of the invention, the invention is not limited to the construction and operation as shown and described. Rather, those skilled in the art will understand that numerous changes and modifications can be made to the present invention without departing from the spirit and scope of the appended claims.

10: electrochemical chip 11: reaction unit
12: electrode 100: chip magazine
110: Chip seating groove 120: Tilting bracket
130: Sample discharge hole 140: Discharge unit
141: communication hole 200: sample supply unit
210: sample storage unit 220: pipette storage unit
221: Pipette 230: Washing liquid storage unit
240: sample transport unit 242: syringe driving unit
245: Pipette fixing pin moving part 250: Pump
260: Multi-valve 300: Voltage application unit
310: Probe 320: Probe moving part
500: Determination unit 600: Magazine tilting unit
610: Drive motor 620: Cam
630: Magazine base 640: Drain hole

Claims (12)

A chip magazine in which a plurality of electrochemical chips, each having a reaction unit that reacts with the target substance to be detected contained in the liquid sample, are placed; and
An automatic sample analysis device including a sample supply unit that sucks a set amount of liquid samples from a sample storage unit where liquid samples are stored and then automatically transfers the liquid samples onto the plurality of reaction units and sequentially dispenses them. According to paragraph 1,
a voltage application unit having a probe to apply voltage by sequentially contacting electrodes of a plurality of electrochemical chips seated in the chip magazine; and
An automatic sample analysis device further comprising a determination unit that determines whether the liquid sample contains the detection target substance by detecting current changes in a plurality of electrochemical chips to which a set voltage is applied through the voltage application unit. According to paragraph 1,
An automatic sample analysis device further comprising a magazine tilting unit for tilting the chip magazine to remove remaining liquid samples that do not react with the detection target substance from the chip magazine. According to paragraph 3,
A tilting bracket is integrally provided on one side of the chip magazine,
The magazine tilting unit,
drive motor; and
An automatic sample analysis device comprising a cam connected to the rotation axis of the drive motor, periodically contacting the tilting bracket as the drive motor rotates, and capable of pushing the tilting bracket upward. According to paragraph 4,
The magazine tilting unit,
In addition to supporting the chip magazine from below, one end of the chip magazine is hinged and rotatable, and a drain hole is provided on the inner bottom surface for discharging the remaining liquid sample that does not react with the detection target substance to the outside. An automatic sample analysis device comprising a magazine base. According to any one of claims 3 to 5,
The chip magazine,
A plurality of chip seating grooves are provided so that the plurality of electrochemical chips are respectively seated, and one side of the plurality of chip seating grooves reacts with the detection target substance in the tilted chip magazine that communicates with the plurality of chip seating grooves, respectively. An automatic sample analysis device characterized in that a plurality of sample discharge holes are provided so that the remaining liquid samples that are not used are discharged. According to clause 6,
An automatic sample analysis device, characterized in that a plurality of discharge portions having communication holes are protruded from the bottom of the chip magazine to communicate with the plurality of sample discharge holes, respectively. According to paragraph 1,
The sample supply unit,
a sample storage unit having a plurality of compartmentalized accommodation spaces in which the liquid samples are stored;
A pipette storage unit in which a plurality of pipettes are stored; and
Provided with a pipette fixing pin for gripping the pipette, gripping any one of the plurality of pipettes stored in the pipette storage unit and transferring it to the sample storage unit, and then sucking the liquid sample from the sample storage unit. An automatic sample analysis device comprising a sample transfer unit that repeatedly transfers and dispenses onto the reaction unit. According to clause 8,
The sample supply unit further includes a washing liquid storage unit in which washing liquid is stored,
The sample transfer unit dispenses the liquid sample onto the reaction unit, separates the pipette currently gripping, grips a new pipette, and then cleans foreign substances not attached to the surface of the reaction unit other than the detection target substance. An automatic sample analysis device, characterized in that it is driven to suck the washing liquid and dispense it onto the plurality of reaction units. According to clause 8,
The sample transfer unit is configured to reciprocate the pipette fixing pin for gripping the pipette in the x, y, and z axes. According to paragraph 2,
The automatic sample analysis device, wherein the voltage application unit reciprocates the probe in the x, y, and z axes. According to paragraph 1,
Automatic sample analysis, characterized in that it further includes an air fan for blowing the remaining liquid sample remaining on the top of the electrochemical chip on the chip magazine and not reacting with the detection target material to the outside of the chip magazine to remove it from the chip magazine. Analysis device.

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