KR20240070082A - Apparatus of lateral shearing phase contrast imaging device - Google Patents

Abstract

The present invention relates to a layer-slipped phase difference image acquisition module device, which includes an optical module that irradiates light output from a light source to a measurement object to obtain reflected light reflected from the measurement object, and a layer-slipped phase difference image acquisition module device that acquires reflected light reflected from the measurement object. In order to do this, the reflected light is polarized and irradiated to the mirror unit, and a signal generator is provided to generate an optical interference signal through the light reflected from the mirror unit.
The layer-sliding phase difference image acquisition module device according to the present invention can acquire an optical interference signal by layer-sliding the reflected light reflected from the measurement object using a polarizer, so the structure is relatively simple, which can reduce the cost and manpower required for manufacturing. there is.
In addition, unlike conventional interferometers, the present invention does not require reference light, so a more reliable optical interference signal can be obtained even when vibration is applied from the outside.
In addition, since the present invention can be configured by installing a light detection module using a layer-push interferometer instead of the light detection unit of a conventional optical microscope, it is possible to simultaneously acquire light intensity images and phase images.

Description

Apparatus of lateral shearing phase contrast imaging device}

The present invention relates to a shearing phase difference image acquisition module device, which is capable of acquiring an optical interference signal based on light obtained by polarizing and diffracting reflected light reflected from a measurement object.

Today, microscope imaging optical system technology is widely used in optics, biotechnology, and many other fields. In particular, the use of a microscope is essential for tissue observation in biotechnology to analyze the structure and characteristics of living cells. However, due to the transparent nature of cells, there are limitations in observing and analyzing cells when using a basic optical microscope, so phase contrast microscopy (phase contrast microscopy) Contrast microscopy and differential interference contrast microscopy are used.

Among these, phase contrast microscopy is a technology used to observe the specimen in three dimensions by converting it into light and dark when the light passing through the material has a phase difference due to the refractive index of the transparent specimen. In addition, differential interference microscopy utilizes a technology that maximizes the contrast of the image of the observed specimen by generating light interference through a birefringent material.

In particular, when observing living cells, it is difficult to observe them with an optical microscope due to the transparent appearance of the cells, so a phase contrast microscope is used. Phase-contrast microscopy obtains images of cells by maximizing contrast between light and dark by detecting the phase of cells through the interference of scattered light and background light, which are divided through the refractive index of cells. However, conventional phase contrast microscopes have the disadvantage of having a complex structure and relatively high manufacturing costs and manpower. Additionally, it is difficult for a conventional phase contrast microscope to acquire light intensity images obtained from a general microscope.

Registered Patent Publication No. 10-1595858: Optical components and phase contrast microscope using optical components

The present invention was created to improve the above problems, and includes a layer-throw phase difference image acquisition module device using a layer-throw interferometer that can generate an optical interference signal by layer-thrive the reflected light reflected from the measurement object using a polarizer. The purpose is to provide.

To achieve the above object, the layer shear phase difference image acquisition module device according to the present invention includes an optical module that irradiates light output from a light source to a measurement object and acquires reflected light reflected from the measurement object, and In order to deflect the reflected light, the reflected light is polarized and irradiated to the mirror, and a signal generator is provided to generate an optical interference signal through the light reflected from the mirror.

The signal generator includes a linear polarizer installed on the optical path of the reflected light incident from the optical module to polarize the reflected light, a beam splitter that irradiates the reflected light polarized by the linear polarizer to the mirror, and the beam splitter and mirror. A first polarizing unit installed between the units, polarizing the reflected light incident on the mirror unit, splitting the reflected light into a plurality of polarized lights diffracted in different directions, and diffracting the polarized light reflected from the mirror unit again; , and is provided with a light detection module that detects polarized light emitted from the polarization unit and generates an optical interference signal based on the detected polarized light.

The first polarization unit divides the reflected light incident from the beam splitter into left-circular polarization and right-circular polarization, which are diffracted in mutually different directions at a predetermined angle with respect to the optical axis line of the reflected light and output to the mirror unit. The reflected left-circularly polarized light and right-circularly polarized light are emitted to the beam splitter in a direction parallel to the optical axis of the reflected light.

The beam splitter splits the reflected light incident from the polarizer into first and second split lights that are emitted in mutually intersecting directions, and the mirror unit includes a first mirror installed on the optical path of the first split light, and It has a second mirror installed on the optical path of the second split light, and the first polarization unit is installed between the beam splitter and the first mirror to polarize the first split light, and divides the first split light into a plurality of A first polarizing member that diffracts and splits polarized light and emits it to the first mirror, diffracts the polarized light reflected from the first mirror again and emits it, and is installed between the beam splitter and the second mirror to produce the second split light. It is provided with a second polarizing member that diffracts and splits the second split light into a plurality of polarized lights and emits it to the second mirror, and diffracts the polarized light reflected from the second mirror and emits it again.

The first polarizing member diffracts the first split light incident from the beam splitter in mutually different directions at a predetermined angle with respect to the optical axis line of the first split light, and outputs the first left circular polarization and the first split light to the reference mirror. Split into 1 right circularly polarized light, and the first left circularly polarized light and the first right circularly polarized light reflected from the reference mirror are emitted to the beam splitter in a direction parallel to the optical axis of the first split light.

The second polarizing member rotates the second split light incident from the beam splitter in mutually different directions at a predetermined angle with respect to the optical axis line of the second split light, and outputs the second left circular polarization and the second split light to the fixed mirror. Split into two right circularly polarized lights, the second left circularly polarized light and the second right circularly polarized light reflected from the fixed mirror are emitted to the beam splitter in a direction parallel to the optical axis of the second split light.

The signal generator may be installed in the mirror unit, and may further include a driver that moves the mirror unit closer to or away from the polarization unit to adjust the amount of layer shedding of the reflected light.

The beam splitter emits the light reflected from the mirror unit to the light detection module, and the light detection module is installed between a light detection unit that detects the light emitted from the beam splitter, the beam splitter, and the camera, A second polarizer is provided to polarize light incident on the camera.

The optical module reflects the light output from the light source and makes it incident on the measurement object, and includes an optical splitter that transmits the reflected light reflected from the measurement object and emits it to the signal generator.

The optical module may further include a collimating lens installed between the light source and the optical distributor to collimate the light output from the light source into parallel light.

The signal generator may further include a tube lens installed between the linear polarizer and the beam splitter to focus the reflected light polarized by the linear polarizer and output it to the beam splitter.

The layer-sliding phase difference image acquisition module device according to the present invention can acquire an optical interference signal by layer-sliding the reflected light reflected from the measurement object using a polarizer, so the structure is relatively simple, which can reduce the cost and manpower required for manufacturing. there is.

In addition, unlike conventional interferometers, the present invention does not require reference light, so a more reliable optical interference signal can be obtained even when vibration is applied from the outside.

In addition, the present invention can be configured by installing a light detection module using a layer-push interferometer instead of the light detection unit of a conventional optical microscope, so it is possible to simultaneously acquire light intensity images and phase images.

1 is a conceptual diagram of a layer shear phase difference image acquisition module device according to an embodiment of the present invention;
Figures 2 and 3 are conceptual diagrams of the first polarization unit of the layer-sliding phase contrast image acquisition module device of Figure 1;
Figure 4 is a diagram of the photodetection module of the layer shear phase difference image acquisition module device of Figure 1;
FIG. 5 is an exemplary diagram of an optical interference signal acquired from the optical detection module of the layer shear phase difference image acquisition module device of FIG. 1;
6 to 13 are analysis images obtained by the layer milling phase contrast image acquisition module device of the present invention for blood vessels;
14 and 15 are analysis images obtained from the layer milling phase contrast image acquisition module device of the present invention for the liver;
Figures 16 to 20 are analysis images obtained from the layer milling phase contrast image acquisition module device of the present invention for the kidney.

Hereinafter, a layer shear phase difference image acquisition module device according to an embodiment of the present invention will be described in detail with reference to the attached drawings. Since the present invention can be subject to various changes and can have various forms, specific embodiments will be illustrated in the drawings and described in detail in the text. However, this is not intended to limit the present invention to a specific disclosed form, and should be understood to include all changes, equivalents, and substitutes included in the spirit and technical scope of the present invention. While describing each drawing, similar reference numerals are used for similar components. In the attached drawings, the dimensions of the structures are enlarged from the actual size for clarity of the present invention.

Terms such as first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The above terms are used only for the purpose of distinguishing one component from another. For example, a first component may be named a second component, and similarly, the second component may also be named a first component without departing from the scope of the present invention.

The terms used in this application are only used to describe specific embodiments and are not intended to limit the invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, terms such as “comprise” or “have” are intended to designate the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but are not intended to indicate the presence of one or more other features. It should be understood that this does not exclude in advance the possibility of the existence or addition of elements, numbers, steps, operations, components, parts, or combinations thereof.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as generally understood by a person of ordinary skill in the technical field to which the present invention pertains. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and should not be interpreted in an ideal or excessively formal sense unless explicitly defined in the present application. No.

1 to 4 show a layer shear phase difference image acquisition module device 100 according to the present invention.

Referring to the drawing, the layer shear phase difference image acquisition module device 100 radiates light output from the light source 200 to the measurement object 10 to obtain reflected light 11 reflected from the measurement object 10. In order to push the reflected light 11 obtained from the optical module 300, the reflected light 11 is polarized and irradiated to the mirror unit 500, It is provided with a signal generator 400 that generates an optical interference signal through reflected light.

The light source 200 generates light to be used to image the measurement object 10. That is, the light source 200 generates light to be incident on the measurement object 10, and irradiates the light with a collimator of the optical module 300, which will be described later. The light source 200 uses a fiber-coupled LED and outputs light with a wavelength of 554 nm.

The optical module 300 includes an optical splitter 310, a collimating lens 320, and an objective lens 330.

The optical splitter 310 is installed on the optical path of the light incident from the light source 200, reflects the light incident from the light source 200 to the measurement object 10, and reflects the reflected light 11 reflected from the measurement object 10. ) is transmitted. Since the optical splitter 310 is a splitter commonly used in the related art to distribute incident light, a detailed description will be omitted.

The collimating lens 320 is installed between the light source 200 and the optical distributor 310 to collimate the light output from the light source 200 into parallel light. The light passing through the collimating lens 320 is incident on the optical splitter 310, and is incident on the measurement object 10 by the optical splitter 310.

The objective lens 330 is installed between the measurement object 10 and the optical splitter 310 to refract and focus the light incident from the optical splitter 310 and make it enter the measurement object 10. The reflected light 11 reflected by the measurement object 10 is incident on the objective lens 330. The objective lens 330 refracts the reflected light 11, collimates it into parallel light, and outputs it to the optical splitter 310. . The reflected light 11 is emitted to the signal generator 400 through the optical splitter 310.

The signal generator 400 includes a linear polarizer 410, a tube lens 420, a beam splitter 430, a first polarizer 440, and a light detection module 450.

The linear polarizer 410 polarizes the reflected light 11 that passes through the optical splitter 310 and outputs it to the beam splitter 430. Since the linear polarizer 410 uses a polarization means generally used in the related art to adjust the polarization state of the incident light to a linear polarization state and output it, a detailed description thereof will be omitted.

The tube lens 420 is installed on the optical path of the reflected light 11 between the linear polarizer 410 and the beam splitter 430, and focuses the reflected light 11 polarized by the linear polarizer to form the beam splitter 430. Enter the military service as

The beam splitter 430 irradiates the reflected light 11 polarized by the linear polarizer 410 to the mirror unit 500, and emits the light reflected from the mirror unit 500 to the light detection module 450. . Here, the beam splitter 430 divides the reflected light 11 incident from the polarizer into first and second split lights 12 and 13 that are emitted in mutually intersecting directions.

Here, the beam splitter 430 linearly polarizes some of the incident reflected light 11 to reflect it in a 90° direction orthogonal to the incident direction, and linearly polarizes the remainder of the reflected light 11 to transmit it along the incident direction. A polarizing beam splitter (PBS) may be applied. Here, the light reflected by the beam splitter 430 in a 90° direction orthogonal to the incident direction is set as the first split light 12, and the light transmitted along the incident direction is set as the second split light 13.

The mirror unit 500 includes a first mirror 510 installed on the optical path of the first split light 12 and reflecting the incident light to the beam splitter 430, and a second split light 13. It is provided with a second mirror 520 that is installed on the optical path and reflects the incident light to the beam splitter 430. Meanwhile, although not shown in the drawing, a driver for moving the first mirror 510 and the second mirror 520 is provided at the bottom of the mirror unit 500, that is, the first mirror 510 and the second mirror 520. (not shown) is installed respectively. The corresponding driver moves the first mirror 510 or the second mirror 520 to be adjacent to or away from the polarization unit along the optical path of the first split light 12 or the second split light 13. The amount of layer shedding of the reflected light 11 can be adjusted by adjusting the separation distance between the mirror unit 500 and the first polarizing unit 440 through the driver.

The first polarizing unit 440 diffracts the reflected light 11 incident from the beam splitter 430 in mutually different directions at a predetermined angle with respect to the optical axis line 14 of the reflected light 11, thereby diffracting the reflected light 11 incident from the beam splitter 430 into mutually different directions. It is divided into left-circularly polarized light and right-circularly polarized light output to 500, and the left-circularly polarized light and right-circularly polarized light reflected from the mirror unit 500 are split in a direction parallel to the optical axis line 14 of the reflected light 11 through the beam splitter ( 430). Here, the first polarizing unit 440 includes a first polarizing member 441 and a second polarizing member 442 made of a polarizing grid.

The first polarizing member 441 is installed between the beam splitter 430 and the first mirror 510 to polarize the first split light 12, and divides the first split light 12 into a plurality of polarizations. It is diffracted and divided and output to the first mirror 510. Additionally, the first polarizing member 441 diffracts the polarized light reflected from the first mirror 510 again and emits it.

The first polarizing member 441 will be described in more detail with reference to FIG. 3 as follows.

The first split light 12 incident from the beam splitter 430 to the first polarizing member 441 is polarized by the first polarizing member 441 and is divided into first left circular polarization 15 and first right circular polarization. It is divided into (16) and output to the first mirror (510). At this time, the first left circular polarization 15 and the first right circular polarization 16 are predetermined with respect to the optical axis line 14 of the first split light 12 according to the diffraction grating characteristics of the first polarization member 441. They have a diffraction angle (θ) and are emitted in different directions. Meanwhile, the first left circularly polarized light 15 and the first right circularly polarized light 16 reflected by the first mirror 510 are re-incident to the first polarizing member 441, and are diffracted by the first polarizing member 441. The first left circularly polarized light 15 and the first right circularly polarized light 16 reflected by the grating characteristic are oriented parallel to the optical axis line 14 of the first split light 12, that is, parallel to the first split light 12. It is emitted from the beam splitter (430). Here, the first left circular polarization 15 and the first right circular polarization 16 are the separation distance between the first polarizing member 441 and the first mirror 510, and the diffraction angle occurring in the first polarizing member 441. (θ) causes floor shedding as shown in Equation 1 below.

Here, △s is the layer shedding value generated from the first left-handed circularly polarized light 15 and the first right-handed circularly polarized light 16, and d is the separation distance between the first polarizing member 441 and the first mirror 510, θ is the diffraction angle of the first left-handed circularly polarized light 15 and the first right-handed circularly polarized light 16. That is, according to Equation 1, when the separation distance between the first mirror 510 and the first polarizing member 441 is changed, the layer shedding value generated in the first left circular polarization 15 and the first right circular polarization 16 You can see that this has changed.

The first left circularly polarized light 15 and the first right circularly polarized light 16 emitted from the first polarizing member 441 are incident on the light detection module 450 by the beam splitter 430.

The second polarizing member 442 is installed between the beam splitter 430 and the second mirror 520 to polarize the second split light 13, and converts the first split light 12 into a plurality of polarized lights. It is diffracted and divided and output to the second mirror 520. Additionally, the second polarizing member 442 diffracts the polarized light reflected from the second mirror 520 again and emits it.

The second polarizing member 442 will be described in more detail as follows.

The second split light 13 incident from the beam splitter 430 to the second polarizing member 442 is polarized by the second polarizing member 442 and is divided into second left circular polarization and second right circular polarization. It is projected to the second mirror (520). At this time, the second left circular polarization and the second right circular polarization have a predetermined diffraction angle (θ) with respect to the optical axis line 14 of the second split light 13 due to the diffraction grating characteristics of the second polarization member 442. and are emitted in different directions. Meanwhile, the second left circularly polarized light and the second right circularly polarized light reflected by the second mirror 520 are re-incident to the second polarizing member 442, and are reflected by the diffraction grating characteristics of the second polarizing member 442. The second left circular polarization and the second right circular polarization are emitted to the beam splitter 430 in a direction parallel to the optical axis line 14 of the second split light 13, that is, parallel to the second split light 13. Here, the second left-handed circular polarization and the second right-handed circular polarization are determined by the separation distance between the second polarizing member 442 and the second mirror 520 and the diffraction angle (θ) generated in the second polarizing member 442. Jungle occurs. At this time, when the separation distance between the second mirror 520 and the second polarizing member 442 is changed, as in the case of the first polarizing member 441 and the first mirror 510, the second left-circular polarization and the second right-circular polarization are generated. The floor push value is changed. The second left circularly polarized light and the second right circularly polarized light emitted from the second polarizing member 442 are incident on the light detection module 450 by the beam splitter 430.

The light detection module 450 is installed between the light detection unit 451, which detects the light emitted from the beam splitter 430, and the beam splitter 430 and the light detection unit 451, and the light incident to the camera It is provided with a second polarization unit 452 that polarizes.

The light detection unit 451 includes a plurality of image sensors 453 for detecting the first left-handed circularly polarized light 15, the first right-handed circularly polarized light 16, the second left-handed circularly polarized light, and the second right-handed circularly polarized light emitted from the beam splitter 430. is provided. The image sensors 453 are preferably arranged in a grid shape. The light detection unit 451 can acquire optical interference signals due to incident left-circularly polarized light and right-handedly polarized light.

The second polarization unit 452 includes a plurality of third polarization members 454 respectively installed on the incident surfaces of the imaging elements where light is incident. The third polarizing member 454 is a linear polarizer with transmission axes of 0˚, 45˚, 90˚, and 135˚. It is preferable that the third polarizing members 454 are arranged in a grid shape corresponding to the imaging elements. Additionally, a micro lens 455 may be installed on the incident surface of the third polarizing member 454 where light is incident from the beam splitter 430.

By using the above-described second polarization unit 452, the light detection unit 451 can simultaneously acquire four phase-shifted optical interference signals as shown in FIG. 5. Here, ' This is an image of an optical interference signal obtained by first left circular polarization (15) and first right circular polarization (16), and 'X+Y' is an image that combines the image of 'X' and the image of 'Y'.

The light detection module 450 is not limited to this, but can be applied to any polarization camera that can acquire an optical interference signal based on incident light.

Meanwhile, the light detection module 450 may further include an image analysis module that generates an image of the measurement object 10 based on the light interference signal acquired by the light detection unit 451. Here, the image analysis module controls the driver to adjust the separation distance between the first mirror 510 and the first polarizing member 441 or the separation distance between the second mirror 520 and the second polarizing member 442. The amount of layer shedding of the reflected light 11, that is, the first and second split lights 12 and 13, can be adjusted.

Here, the image analysis module can use phase-shifted optical interference signals to extract the phase of the optical interference signal through Equation 2 below.

Here, Φ is the phase of the specimen being measured, and I _0˚ , I _45˚ , I _90˚ , I _135˚ are the light intensity according to each polarization direction.

In addition, the image analysis module can also acquire images based on light intensity measured in a conventional microscope through visibility detection, as shown in Equation 3 below.

Meanwhile, in FIGS. 6 to 13 , analysis images obtained by the layer milling phase contrast image acquisition module device 100 of the present invention for blood vessels, that is, bright field image, phase contrast image, and image visibility, are posted. In addition, Figures 14 and 15 show the bright field image, phase contrast image, and image visibility acquired by the layer milling phase contrast image acquisition module device 100 of the present invention for the liver. And, in FIGS. 16 to 20, bright field images, phase contrast images, and image visibility acquired by the layer milling phase contrast image acquisition module device 100 for the kidney of the present invention are posted. Referring to the drawings, it can be seen that the layer shear phase difference image acquisition module device 100 according to the present invention can acquire an analysis image with high reliability and accuracy even if the measurement object is fine or has high transparency.

The layer shearing phase difference image acquisition module device 100 according to the present invention configured as described above can acquire an optical interference signal by shearing the reflected light 11 reflected from the measurement object 10 using a polarizer, and thus has a structure. It is relatively simple and can reduce manufacturing costs and manpower.

In addition, unlike conventional interferometers, the present invention does not require reference light, so a more reliable optical interference signal can be obtained even when vibration is applied from the outside.

In addition, the present invention can be configured by installing a light detection module using a layer-push interferometer instead of the light detection unit of a conventional optical microscope, so it is possible to simultaneously acquire light intensity images and phase images.

The description of the presented embodiments is provided to enable any person skilled in the art to use or practice the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be applied to other embodiments without departing from the scope of the invention. Thus, the present invention is not limited to the embodiments presented herein, but is to be construed in the broadest scope consistent with the principles and novel features presented herein.

100: layer shear phase contrast image acquisition module device 200: light source
300: Optical module 310: Optical splitter
320: collimating lens 330: objective lens
400: signal generator 410: linear polarizer
420: Tube lens 430: Beam splitter
440: first polarization unit 441: first polarization member
442: second polarizing member 450: light detection module
451: light detection unit 452: second polarization unit
500: Mirror unit 510: First mirror
520: Second mirror

Claims (11)

An optical module that irradiates light output from a light source to a measurement object and obtains reflected light reflected from the measurement object; and
In order to deflect the reflected light obtained from the optical module, the reflected light is polarized and irradiated to the mirror unit, and a signal generator that generates an optical interference signal through the light reflected from the mirror unit; comprising a.
Laminar phase contrast image acquisition module device.
According to paragraph 1,
The signal generator
A linear polarizer installed on the optical path of the reflected light incident from the optical module to polarize the reflected light;
a beam splitter that irradiates reflected light polarized by the linear polarizer to the mirror unit;
It is installed between the beam splitter and the mirror unit, polarizes the reflected light incident on the mirror unit, splits the reflected light into a plurality of polarized lights diffracted in mutually different directions, and re-diffracts the polarized light reflected from the mirror unit. First polarization unit;
A light detection module that detects polarized light emitted from the polarization unit and generates an optical interference signal based on the detected polarized light,
Laminar phase contrast image acquisition module device.
According to paragraph 2,
The first polarization unit divides the reflected light incident from the beam splitter into left-circular polarization and right-circular polarization, which are diffracted in mutually different directions at a predetermined angle with respect to the optical axis line of the reflected light and output to the mirror unit. Emitting the reflected left-circularly polarized light and right-handedly polarized light to the beam splitter in a direction parallel to the optical axis line of the reflected light,
Laminar phase contrast image acquisition module device.
According to paragraph 2,
The beam splitter splits the reflected light incident from the polarizer into first and second split lights that are emitted in mutually intersecting directions,
The mirror unit includes a first mirror installed on the optical path of the first split light and a second mirror installed on the optical path of the second split light,
The first polarizer
It is installed between the beam splitter and the first mirror to polarize the first split light, and the first split light is diffracted and divided into a plurality of polarized lights to be emitted to the first mirror, and the polarized light reflected from the first mirror A first polarizing member that diffracts again and emits light; and
It is installed between the beam splitter and the second mirror to polarize the second split light, and the second split light is diffracted and split into a plurality of polarized lights to be emitted to the second mirror, and the polarized light reflected from the second mirror A second polarizing member that diffracts again and emits light,
Laminar phase contrast image acquisition module device.
According to clause 4,
The first polarizing member diffracts the first split light incident from the beam splitter in mutually different directions at a predetermined angle with respect to the optical axis line of the first split light, and outputs the first left circular polarization and the first split light to the reference mirror. Splitting into 1 right circular polarization, and emitting the first left circular polarization and the first right circular polarization reflected from the reference mirror to the beam splitter in a direction parallel to the optical axis line of the first split light,
Laminar phase contrast image acquisition module device.
According to clause 4,
The second polarizing member rotates the second split light incident from the beam splitter in mutually different directions at a predetermined angle with respect to the optical axis line of the second split light, and outputs the second left circular polarization and the second split light to the fixed mirror. Splitting the second right circular polarization into two right circular polarizations, and emitting the second left circular polarization and the second right circular polarization reflected from the fixed mirror to the beam splitter in a direction parallel to the optical axis line of the second split light,
Laminar phase contrast image acquisition module device.
According to paragraph 2,
The signal generator is installed in the mirror unit, and further includes a driver that moves the mirror unit adjacent to or away from the polarization unit to adjust the amount of layer shedding of the reflected light.
Laminar phase contrast image acquisition module device.
According to paragraph 2,
The beam splitter emits the light reflected from the mirror unit to the light detection module,
The light detection module is
a light detection unit that detects light emitted from the beam splitter; and
A second polarizer installed between the beam splitter and the camera and polarizing the light incident on the camera,
Laminar phase contrast image acquisition module device.
According to claim 1 or 2,
The optical module reflects the light output from the light source and makes it incident on the measurement object, and includes an optical splitter that transmits the reflected light reflected from the measurement object and emits it to the signal generator.
Laminar phase contrast image acquisition module device.
According to clause 8,
The optical module further includes a collimating lens installed between the light source and the optical distributor to collimate the light output from the light source into parallel light,
Laminar phase contrast image acquisition module device.
According to paragraph 2,
The signal generator further includes a tube lens installed between the linear polarizer and the beam splitter to focus the reflected light polarized by the linear polarizer and emit it to the beam splitter.
Laminar phase contrast image acquisition module device.

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