KR20240055718A - 조작된 폴리머라제 - Google Patents
조작된 폴리머라제 Download PDFInfo
- Publication number
- KR20240055718A KR20240055718A KR1020247001936A KR20247001936A KR20240055718A KR 20240055718 A KR20240055718 A KR 20240055718A KR 1020247001936 A KR1020247001936 A KR 1020247001936A KR 20247001936 A KR20247001936 A KR 20247001936A KR 20240055718 A KR20240055718 A KR 20240055718A
- Authority
- KR
- South Korea
- Prior art keywords
- polymerase
- amino acid
- nucleotide
- nucleic acid
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163212540P | 2021-06-18 | 2021-06-18 | |
| US63/212,540 | 2021-06-18 | ||
| US17/705,011 | 2022-03-25 | ||
| US17/705,043 | 2022-03-25 | ||
| US17/705,011 US20220403351A1 (en) | 2021-06-18 | 2022-03-25 | Engineered polymerases |
| US17/705,020 | 2022-03-25 | ||
| US17/705,043 US12139727B2 (en) | 2021-06-18 | 2022-03-25 | Engineered polymerases |
| US17/705,020 US12270056B2 (en) | 2021-06-18 | 2022-03-25 | Engineered polymerases |
| PCT/US2022/034021 WO2022266462A2 (en) | 2021-06-18 | 2022-06-17 | Engineered polymerases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20240055718A true KR20240055718A (ko) | 2024-04-29 |
Family
ID=82558168
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020247001936A Pending KR20240055718A (ko) | 2021-06-18 | 2022-06-17 | 조작된 폴리머라제 |
Country Status (8)
| Country | Link |
|---|---|
| EP (2) | EP4355867A2 (https=) |
| JP (1) | JP2024522795A (https=) |
| KR (1) | KR20240055718A (https=) |
| AU (1) | AU2022291874A1 (https=) |
| CA (1) | CA3223112A1 (https=) |
| IL (1) | IL309440A (https=) |
| TW (1) | TW202317760A (https=) |
| WO (1) | WO2022266462A2 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019241305A1 (en) | 2018-06-12 | 2019-12-19 | Element Biosciences, Inc. | Improved reverse transcriptase for nucleic acid sequencing |
| US10704094B1 (en) | 2018-11-14 | 2020-07-07 | Element Biosciences, Inc. | Multipart reagents having increased avidity for polymerase binding |
| US10768173B1 (en) | 2019-09-06 | 2020-09-08 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| WO2020102766A2 (en) | 2018-11-15 | 2020-05-22 | Element Biosciences, Inc. | Methods for generating circular nucleic acid molecules |
| US12313627B2 (en) | 2019-05-01 | 2025-05-27 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| US12391929B2 (en) | 2019-05-24 | 2025-08-19 | Element Biosciences, Inc. | Polymerase-nucleotide conjugates for sequencing by trapping |
| US11287422B2 (en) | 2019-09-23 | 2022-03-29 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| US12359193B2 (en) | 2022-03-04 | 2025-07-15 | Element Biosciences, Inc. | Single-stranded splint strands and methods of use |
| US12371743B2 (en) | 2022-03-04 | 2025-07-29 | Element Biosciences, Inc. | Double-stranded splint adaptors and methods of use |
| AU2022316142A1 (en) | 2021-07-21 | 2024-02-22 | Element Biosciences, Inc. | Optical systems for nucleic acid sequencing and methods thereof |
| US12606819B2 (en) | 2022-07-05 | 2026-04-21 | Element Biosciences, Inc. | PCR-free library preparation using double-stranded splint adaptors and methods of use |
| AU2023343187A1 (en) | 2022-09-12 | 2025-03-13 | Element Biosciences, Inc. | Double-stranded splint adaptors with universal long splint strands and methods of use |
| WO2025024465A1 (en) * | 2023-07-24 | 2025-01-30 | Element Biosciences, Inc. | On-support circularization and amplification for generating immobilized nucleic acid concatemer molecules |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1340806C (en) | 1986-07-02 | 1999-11-02 | James Merrill Prober | Method, system and reagents for dna sequencing |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US7211390B2 (en) | 1999-09-16 | 2007-05-01 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| GB0321306D0 (en) * | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| EP2230316A1 (en) | 2005-02-01 | 2010-09-22 | AB Advanced Genetic Analysis Corporation | Nucleic acid sequencing by performing successive cycles of duplex extension |
| US20110045489A1 (en) * | 2008-04-22 | 2011-02-24 | New England Biolabs, Inc. | Polymerases for Incorporating Modified Nucleotides |
| EP3497236B1 (en) | 2016-08-15 | 2021-11-03 | Omniome, Inc. | Method for sequencing nucleic acids |
| WO2018148727A1 (en) * | 2017-02-13 | 2018-08-16 | Qiagen Waltham Inc. | Polymerase enzyme from 9°n |
| US11512295B2 (en) * | 2019-09-12 | 2022-11-29 | Singular Genomics Systems, Inc. | Modified thermoccocus polymerases |
| CA3196800A1 (en) * | 2020-10-30 | 2022-05-05 | Element Biosciences, Inc. | Reagents for massively parallel nucleic acid sequencing |
| CN119317711A (zh) * | 2022-04-07 | 2025-01-14 | 元素生物科学公司 | 具有反应性基团的多价结合组合物 |
-
2022
- 2022-06-17 IL IL309440A patent/IL309440A/en unknown
- 2022-06-17 EP EP22741923.1A patent/EP4355867A2/en not_active Withdrawn
- 2022-06-17 EP EP25202557.2A patent/EP4650457A3/en active Pending
- 2022-06-17 JP JP2023577945A patent/JP2024522795A/ja active Pending
- 2022-06-17 CA CA3223112A patent/CA3223112A1/en active Pending
- 2022-06-17 AU AU2022291874A patent/AU2022291874A1/en active Pending
- 2022-06-17 KR KR1020247001936A patent/KR20240055718A/ko active Pending
- 2022-06-17 TW TW111122750A patent/TW202317760A/zh unknown
- 2022-06-17 WO PCT/US2022/034021 patent/WO2022266462A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022266462A9 (en) | 2023-07-13 |
| TW202317760A (zh) | 2023-05-01 |
| WO2022266462A2 (en) | 2022-12-22 |
| AU2022291874A1 (en) | 2024-01-18 |
| EP4355867A2 (en) | 2024-04-24 |
| EP4650457A3 (en) | 2026-03-11 |
| CA3223112A1 (en) | 2022-12-22 |
| EP4650457A2 (en) | 2025-11-19 |
| JP2024522795A (ja) | 2024-06-21 |
| WO2022266462A3 (en) | 2023-05-04 |
| IL309440A (en) | 2024-02-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
St.27 status event code: A-0-1-A10-A15-nap-PA0105 |
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| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
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| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
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| R18-X000 | Changes to party contact information recorded |
St.27 status event code: A-3-3-R10-R18-oth-X000 |
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| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
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| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
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| PG1501 | Laying open of application |
St.27 status event code: A-1-1-Q10-Q12-nap-PG1501 |
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| R18-X000 | Changes to party contact information recorded |
St.27 status event code: A-3-3-R10-R18-oth-X000 |
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| A201 | Request for examination | ||
| E13-X000 | Pre-grant limitation requested |
St.27 status event code: A-2-3-E10-E13-lim-X000 |
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| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
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| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
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| PA0201 | Request for examination |
St.27 status event code: A-1-2-D10-D11-exm-PA0201 |