KR20240054087A - Method for improving the functionality of peanut shells comprising plasma treatment - Google Patents

Abstract

The present invention provides a method for producing a peanut skin extract with improved functionality, comprising the steps of preparing peanut skin, treating the peanut skin with plasma, and extracting the plasma-treated peanut skin; A method for improving the functionality of peanut skin comprising the above steps; Peanut shells whose functionality has been improved by the above method; A cosmetic composition for skin whitening or wrinkle improvement comprising the peanut shell; and quasi-drug compositions.

Description

Method for improving the functionality of peanut shells comprising plasma treatment}

The present invention provides a method for producing a peanut skin extract with improved functionality, comprising the steps of preparing peanut skin, treating the peanut skin with plasma, and extracting the plasma-treated peanut skin; A method for improving the functionality of peanut skin comprising the above steps; Peanut shells whose functionality has been improved by the above method; A cosmetic composition for skin whitening or wrinkle improvement comprising the peanut shell; and quasi-drug compositions.

Peanut (Arachis hypogaea L.) is an annual herbaceous plant belonging to the Rosales legume family (Fabaceae) and is cultivated as an oil raw material crop due to its high oil content. The fat content of peanuts is 35.8-54.2%, with doubly unsaturated fatty acids accounting for more than 80%, and the protein content is high at 16.2-36%, so they are also used as protein food ingredients (Lee et al., 1988; Lim et al., 2004). , Recently, it has been attracting attention as a health food because it contains a large amount of phenolic compounds, including vitamins, minerals, resveratrol (3,5,4'-trihydroxystilbene), coumaroyl-rhamnose, and pcoumaric acid derivatives (Limmongkon et al., 2017). When a peanut bears fruit, the bottom of the ovary burrows into the soil to form a pod. The outer shell of the peanut that surrounds the peanut protects the fruit from external physical and chemical attacks such as moisture, ultraviolet rays, microorganisms, and pests. It has a self-defense function. The outer skin of blood peanuts, which accounts for 35-40% of the weight of blood peanuts, contains various functional substances such as plant sterols, phenolic acids and flavonoids, but is recognized as a by-product and most of it is discarded or used as feed (Qiu et al., 2012) .

On the other hand, when energy is applied to a gas composed of atoms or molecules, the electrically neutral atoms or molecules become ionized to become a collection of electrons and ions, and these charged particles become a state of matter showing collective motion. This state of matter is called plasma. Plasma is classified according to temperature and density into nuclear fusion plasma in the high-temperature and high-density region, process application plasma in the low-temperature region, and space plasma in the low-density region. Nuclear fusion plasma is a high-temperature plasma of about 100 million degrees in a completely ionized state of ions and atoms, while low-temperature plasma is composed of a partially ionized state with a mixture of non-ionized neutral atoms or molecules.

Among low-temperature plasmas, atmospheric pressure plasma is a technology that generates plasma at atmospheric pressure, and includes dielectric barrier discharge (DBD), corona discharge, microwave discharge, and arc discharge. ), etc. In particular, compared to low-pressure process plasma, atmospheric pressure plasma can be generated and utilized without a vacuum system depending on the purpose of use, so it is economical due to low installation and maintenance costs, and is known as a non-polluting and environmentally friendly technology because it does not generate waste.

Plasma generates atoms with high energy, highly reactive radicals (-OH, -H, -HO ₂ , H ₂ O ₂ ), and ozone (O ₃ ) by electric discharge in gases or liquids such as air. It is reported to have sterilizing power, and most recent cases of applying this principle for sanitation in the food field (Kim, B. et al., Food Microbiol 28, 9-13) have led to the development of functional materials. Cases of applying plasma technology for this purpose are limited.

Research on peanut by-products published to date includes establishment of optimal extraction conditions for functional components of the outer skin, research on testing the biological activity of the seed coat by variety, and research on the functional components and physiological activity of the outer skin according to the extraction solvent and variety. have been published, but no research has been reported on the improvement of physiological functionality by applying plasma technology to the outer shell of peanuts.

Against this background, the present inventors made research efforts to develop functional materials using discarded peanut skins, and as a result, functional ingredients such as total polyphenols and flavonoids of peanut skins treated with atmospheric pressure plasma were improved, and antioxidant activity and tyrosinase were improved. The present invention was completed by confirming that whitening and wrinkle improvement were increased due to increased enzyme inhibitory activity.

One object of the present invention is to prepare peanut shells; Treating the peanut shell with plasma; And to provide a method for producing a peanut skin extract with improved functionality, including the step of extracting the plasma-treated peanut skin.

Another object of the present invention is to prepare peanut shells; Treating the peanut shell with plasma; And to provide a method for improving the functionality of peanut skin, comprising extracting the plasma-treated peanut skin.

Another object of the present invention is to provide peanut shells with improved functionality through the above improvement method.

Another object of the present invention is to provide a cosmetic composition for skin whitening or wrinkle improvement containing the peanut skin extract.

Another object of the present invention is to provide a quasi-drug composition for skin whitening or wrinkle improvement containing the peanut skin extract.

This is explained in detail as follows. Meanwhile, each description and embodiment disclosed in the present invention may also be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in the present invention fall within the scope of the present invention. Additionally, the scope of the present invention cannot be considered limited by the specific description described below.

One aspect of the present invention for achieving the above object includes preparing peanut outer shell; Treating the peanut shell with plasma; And it provides a method for producing a peanut skin extract with improved functionality, including the step of extracting the plasma-treated peanut skin.

In addition, another aspect of the present invention for achieving the above object includes preparing peanut outer shell; Treating the peanut shell with plasma; And it provides a method for improving the functionality of peanut outer skin, comprising extracting the plasma-treated peanut outer skin.

The term "peanut" in the present invention is an annual herbaceous plant belonging to the Rosales legume family (Fabaceae), and is attracting attention as a health food because it contains a large amount of fat, protein, vitamins, minerals, phenolic compounds, etc. The “peanut shell” accounts for 35-40% of the weight of the peanut and contains various functional substances such as plant sterols, phenolic acids, and flavonoids, and has various pharmaceutical uses such as dementia prevention, anti-inflammatory, anti-diabetic, etc. Despite active research, it is still considered a by-product and most of it is discarded or used as feed.

In the present invention, the peanut outer shell may be purchased and used commercially, or may be used as one collected or cultivated in nature, but is not limited thereto.

The term "plasma" in the present invention is a form of matter known as the fourth state of matter other than solid, liquid, and gas, and may mean low-temperature plasma (or non-equilibrium plasma). The low-temperature plasma refers to a plasma in a state in which the energy of electrons among the ions and electrons constituting the plasma is greater than the energy of the ions. In the present invention, the plasma is generated by a dielectric barrier discharge (DBD) method, and specifically, the electrical characteristic of generating plasma ions by passing electricity through electrodes on both sides of a dielectric can be utilized. The DBD method is widely used in industry because it can be discharged at atmospheric pressure (normal pressure) and room temperature.

In the present invention, the plasma discharge method may specifically mean selected from AT-DBD, AT-DBD-gas substitution, and RF jet, and may more specifically mean AT-DBD-gas substitution, but is limited thereto. It doesn't work.

In one embodiment of the present invention, as a result of comparing the functional ingredient content and antioxidant activity of peanut outer skin according to the plasma discharge method, the total polyphenol and flavonoid content as functional ingredients was determined by AT-DBD-gas displacement discharge (atmospheric pressure dielectric). In the barrier discharge-gas exchange (AT-DBD-gas exchange) method, it was 0.41 GAE mg/g and 0.09 CE mg/g, respectively, which were higher than those of other treatments (Table 1), and in particular, increased by 1.32 and 3.00 times, respectively, compared to the control group. In addition, antioxidant activity was significantly improved in AT-DBD-gas substitution and RF jet discharge methods in DPPH, ABTS, and FRAP activities compared to the control (Table 2).

According to the above results, AT-DBD-gas substitution discharge method was selected as the optimal plasma processing model of the present invention.

The term "functionality" of the present invention may refer to functional ingredients such as polyphenols and flavonoids contained in the outer shell of peanuts, antioxidant activity, and enzyme inhibitory activity related to skin aging, and the enzyme related to skin aging is specifically , tyrosinase, elastase, collagenase, or a combination thereof, but is not limited thereto. Additionally, “improved functionality” may mean that the functional ingredients and activity are increased compared to those inherent in the peanut outer shell.

In the present invention, when the active species (radical) generated during the plasma treatment is O ₃ , this may mean that functional ingredients, antioxidant activity, and enzyme inhibitory activity related to skin aging are increased.

In addition, in the present invention, the plasma treatment temperature may be specifically, room temperature to 180°C, more specifically, 100°C to 170°C, more specifically, 120°C to 160°C, and most Specifically, it may be 140°C to 160°C, but is not limited thereto.

The term "extract" in the present invention refers to the resulting liquid component obtained by immersing the desired substance in various solvents and then extracting it at room temperature or at a heated state for a certain period of time, and the solid component obtained by removing the solvent from the liquid component. it means. In addition, in addition to the above results, it can be comprehensively interpreted to include dilutions of the results, concentrates thereof, crude preparations, purifications, etc. of the above results. Accordingly, the extract of the peanut skin provided by the present invention is an extract obtained by extraction treatment, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a crude product or purified product of the extract, or a mixture thereof, etc. , it can be interpreted to include the extract itself and all types of extracts that can be formed using the extract.

The extraction method for the extract of the present invention is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include hot water extraction, ultrasonic extraction, filtration, and reflux extraction, which may be performed alone or by combining two or more methods.

In the present invention, the type of solvent used for the extraction is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water, alcohol, or a mixed solvent thereof, and these may be used alone or in a mixture of one or more types, and specifically, water may be used. When alcohol is used as a solvent, alcohol having 1 to 4 carbon atoms can be used, and more specifically, ethanol can be used.

In the present invention, the extract may mean extraction of plasma-treated peanut skin and 80% ethanol at a ratio of 0.5 to 2 to 9.5 to 8, and more specifically, extraction at a ratio of 1 to 9. However, it is not limited to this.

In addition, the extract may be prepared and used in concentrated and freeze-dried form after extraction, but is not limited thereto.

In one embodiment of the present invention, a peanut skin extract was prepared by extracting plasma-treated peanut skin and 80% ethanol at a ratio of 1:9 for 24 hours (FIG. 1).

In addition, in the present invention, the peanut skin extract affects its functional ingredients, antioxidant activity, and enzyme inhibitory activity depending on the plasma treatment conditions, specifically the active species (radicals) generated during plasma treatment and the treatment temperature. It can mean something that can go crazy.

In one embodiment of the present invention, as a result of examining the functional components of peanut skin extract according to plasma treatment conditions, specifically, the treatment method and treatment temperature when the active species is O ₃ or _NO As a result of treating plasma with different conditions, O ₃ treatment increased the total polyphenol and flavonoid content of peanut skin. In particular, the 150 ℃ treatment group showed the highest content, and the O ₃ and 150 ℃ treatment conditions increased the total polyphenol and flavonoid content. and was confirmed to be most effective in increasing flavonoid content (Table 3).

In addition, in one embodiment of the present invention, the antioxidant activity of peanut skin extract according to plasma treatment conditions was compared and reviewed through reducing power assay (FRAP), DPPH radical scavenging ability, and metal chelating effect test. As a result, O ₃ , 150 It was excellent in the following order: ℃ > O ₃ 25 > NO _x , 150℃ ≥ control (Table 4).

The above results may mean that antioxidant activity increased due to an increase in the total polyphenol and flavonoid content of peanut outer skin due to plasma treatment.

This increase in the content of the above-mentioned functional ingredients improves the antioxidant activity and whitening and wrinkle-improving activity of the peanut skin extract, suggesting that it can be used as a cosmetic or quasi-drug material.

Another aspect of the present invention for achieving the above object provides a peanut outer skin extract with improved functionality by the above functionality improvement method.

At this time, the “peanut shell” and “extract” are as described above.

Another aspect of the present invention for achieving the above object provides a cosmetic composition for skin whitening or wrinkle improvement comprising a peanut skin extract with improved functionality.

At this time, the “peanut shell” and “extract” are as described above.

As used herein, the term “whitening” refers to whitening the skin, and may refer to any action that inhibits or prevents the deposition of melanin on the skin by inhibiting the synthesis of melanin.

The melanin is a dark brown granular pigment and is produced by organelles called melanosomes within melanocytes. Melanin is present in skin, hair, and eyes, and skin color is determined by the amount of melanin. The greater the amount of melanin, the darker the skin color.

In the present invention, the whitening may be achieved through the inhibitory activity of tyrosinase, a melanin-producing enzyme.

The term "wrinkle" in the present invention refers to fine lines that occur as the skin ages, and can be caused by genes, a decrease in collagen present in the skin dermis, external environment, etc.

In the present invention, the improvement of wrinkles may be achieved by inhibiting the activity of elastase and collagenase, enzymes that cause wrinkles by damaging intracellular elastin and collagen molecules.

The term "improvement" of the present invention means suppressing or inhibiting the symptoms of skin deposition or wrinkles, or alleviating the symptoms of skin deposition or wrinkles that have already appeared, using the composition of the present invention.

In one embodiment of the present invention, in order to examine the possibility of turning peanut skin into a cosmetic material, the tyrosinase activity related to skin whitening was compared according to plasma treatment conditions. As a result, the O ₃ , 150°C treatment group showed the best tyrosinase inhibitory activity. , it was confirmed that the inhibitory activity increased by about 20% compared to the control group (Table 5).

In addition, in one embodiment of the present invention, in order to examine the possibility of turning peanut skin into a cosmetic material, the elastase and collagenase inhibitory activities related to skin wrinkles were compared according to plasma treatment conditions. As a result, O ₃ , 150° C. , it was confirmed that the inhibitory activity of each enzyme was the best in the treatment group (Table 5).

In the present invention, the cosmetic composition includes a solution, external ointment, cream, foam, nutritional lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid cleanser, bath additive, sunscreen cream, sun oil, Can be prepared in a formulation selected from the group consisting of suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays. Not limited.

The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers that are blended with general skin cosmetics, and common ingredients include, for example, oil, water, surfactant, moisturizer, lower alcohol, and thickener. , chelating agents, pigments, preservatives, fragrances, etc. may be appropriately mixed, but are not limited thereto. Cosmetically acceptable carriers included in the cosmetic composition of the present invention vary depending on the formulation.

When the formulation of the present invention is an ointment, paste, cream or gel, the carrier ingredient may be animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures of these can be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silcate, polyamide powder, or mixtures thereof may be used as the carrier ingredient, and especially in the case of a spray, chloro May contain propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-Butyl glycol oils may be used, in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, aliphatic esters of glycerol, fatty acid esters of polyethylene glycol or sorbitan. there is.

When the formulation of the present invention is a suspension, the carrier components include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and miso. Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

On the other hand, when the formulation of the present invention is a capsule, it may be formulated in the form of an alginate capsule, agar capsule, gelatin capsule, wax capsule, or double capsule. It is not limited to this.

In addition, the cosmetic composition of the present invention contains commonly used auxiliaries, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic activators, preservatives, antioxidants, solvents, and fragrances, in addition to peanut skin extract, fractions thereof, or compounds isolated therefrom. , fillers, blocking agents, pigments, odorants, and dyes.

Another aspect of the present invention for achieving the above object provides a quasi-drug composition for skin whitening or wrinkle improvement comprising a peanut skin extract with improved functionality.

At this time, the “peanut shell,” “extract,” “whitening,” “wrinkle,” and “improvement” are the same as described above.

The quasi-drug composition may have the function of helping to lighten the color of melanin pigment deposited on the skin, and prevents excessive deposition of melanin pigment on the skin by inhibiting tyrosinase activity, thereby suppressing the occurrence of skin pigmentation disease. It may have a function that helps in whitening. In addition, it may have the function of helping to improve wrinkles caused by damage to intracellular elastin and collagen molecules, and it helps improve skin wrinkles by suppressing or inhibiting the occurrence of skin wrinkles by inhibiting elastase or collagenase activity. can give.

In addition to the above ingredients, the quasi-drug composition of the present invention may further include pharmaceutically acceptable carriers, excipients, or diluents as needed. The pharmaceutically acceptable carrier, excipient, or diluent is not limited as long as it does not impair the effect of the present invention, and includes, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. It can be included.

The quasi-drug composition of the present invention may include, but is not limited to, disinfectant cleaners, shower foams, ointments, wet tissues, coating agents, etc., and the formulation method, dosage, usage method, components, etc. of the quasi-drugs are commonly known in the technical field. It can be appropriately selected from the techniques.

According to the present invention, it was confirmed that functionality such as skin whitening or wrinkle improvement can be improved as the total polyphenols and flavonoids, antioxidant activity, and enzyme inhibitory activity of the plasma-treated peanut skin are increased. Therefore, a cosmetic composition using the same can be improved. Alternatively, it can be used as a quasi-drug composition.

Figure 1 shows a schematic diagram and processing method of a dielectric barrier discharge gas substitution system.
Figure 2 analyzes the main active species generated by plasma generation.
Figure 3 shows the results of functional ingredients and physiological activity of peanut skin according to plasma treatment conditions through heatmap and VIP analysis.

Hereinafter, the present invention will be described in more detail through examples. These examples are for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples.

Experimental Example 1. Plasma treatment method

Low-temperature plasma technology was applied as a way to improve the functionality of peanut shells, which are recognized as waste. The plasma used a dielectric barrier discharge (DBD) method that utilizes the electrical characteristics of generating plasma ions by passing electricity through electrodes on both sides of a dielectric that can be discharged at atmospheric pressure and room temperature.

Specifically, as shown in the schematic diagram shown in FIG. 1, chemically active species are stabilized by generating plasma in the chamber containing the peanut shell (sample) while all valves (V1, V2) outside the plasma chamber are closed. and at the same time lowered the pressure in the chamber to 5 to 10 Torr. Afterwards, the valve V3 on the gas outlet side of the gas-tight chamber was closed, and V1 and V2 were opened together to slowly inject the plasma active gas into the gas-tight chamber. At this time, the time required from low pressure to atmospheric pressure due to plasma active gas injection was 7.5 minutes, and the above process was repeated a total of three times to treat the peanut outer shell with plasma.

Experimental Example 2. Plasma active species analysis

In-situ gas analysis (Optical Absorption Spectroscopy) was applied to each chamber to investigate changes in the density of major active species without gas sampling. At this time, the number density of each active species was expressed according to the treatment time.

As a result, as shown in Figure 2, ozone (O ₃ ), nitrogen monoxide (NO), and nitrogen dioxide (NO ₂ ) were detected as main gases, and the activity varied in each chamber according to the plasma treatment method and sequence of Experimental Example 1. The number density of species was expressed numerically along with pressure changes.

Experimental Example 3. Preparation of peanut skin extract

To prepare an extract of the plasma-treated peanut skin in Example 1, the dried and ground peanut skin and solvent were extracted for 24 hours using 80% ethanol as a solvent at a ratio of 1:9. After extraction, it was filtered and concentrated under reduced pressure, then re-dissolved in dimethyl sulfoxide (DMSO) at a concentration of 100 mg/mL and used in the experiment (yield 3-4%).

Experimental Example 4. Statistical analysis

For all data, the average and standard deviation of each measurement group were calculated after three replicate experiments, and the presence or absence of differences between each treatment group was analyzed using one way ANOVA (analysis of variance), followed by Duncan's multiple range test at a confidence interval of P<0.05. Significance was tested using .

Example 1. Comparison of functional ingredient content and antioxidant activity according to plasma discharge method

We compared and analyzed the functional ingredients and antioxidant activity contained in peanut skin extract according to the discharge method of treating the peanut skin with plasma.

Total polyphenol and flavonoid content

First, the content of functional ingredients according to the discharge method of plasma treatment of peanut outer shell was confirmed through comparative analysis of total polyphenol and flavonoid content. Samples for analysis were diluted to 1 mg/mL using DMSO and used for analysis.

Specifically, the total polyphenol content was determined using the Folin-Ciocalteu method. To 100 μL of the extract, 2 mL of 2% Na ₂ CO ₃ and 100 μL of 50% Folin-Ciocalteu reagent were added and reacted for 30 minutes, and then the absorbance was measured at 750 nm. The total polyphenol content was calculated using gallic acid as a standard material.

The total flavonoid content was determined using the AlCl ₃ method. To 75 μL of the extract, 300 μL of distilled water, 22.5 μL of NaNO ₃ , 45 μL of NaCl ₃ , and 150 μL of 1M NaOH were added and reacted for 20 minutes, and the absorbance was measured at 510 nm. Total flavonoid content was calculated using catechin as a standard material.

Its total polyphenol and flavonoid content is shown in Table 1 below ( p < 0.05

).

As shown in Table 1, the total polyphenol and flavonoid content was higher than that of other treatments at 0.41 GAE mg/g and 0.09 CE mg/g, respectively, in the AT-DBD-gas substitution discharge method, especially 1.32 and 1.32, respectively, compared to the control. It was confirmed that there was a 3.00-fold increase.

antioxidant activity

Next, the antioxidant activity according to the discharge method of treating the peanut skin with plasma was confirmed through comparative analysis of FRAP (reducing power assay), DPPH radical scavenging ability, and metal chelating effect test. Samples for analysis were diluted to 1 mg/mL using DMSO and used for analysis.

Specifically, in the FRAP assay, 180 μL of FRAP reagent and 90 μL of distilled water were added to 30 μL of the extract, reacted for 10 minutes, and absorbance was measured at 593 nm.

DPPH radical scavenging ability was measured by adding 200 μL of DPPH reagent to 20 μL of the extract, reacting for 30 minutes, and measuring the absorbance at 520 nm.

To test the metal chelating effect, 2.5 μL of 2 mM FeCl ₂ , 5 μL of 5 mM ferrozine, and 160 μL of ethanol were added to 25 μL of the extract, reacted for 10 minutes, and absorbance was measured at 562 nm.

Its FRAP, DPPH radical scavenging ability and metal chelating results are shown in Table 2 below ( p<0.05 ).

As shown in Table 2 above, it was confirmed that the antioxidant activity of FRAP, DPPH, and metal chelating effects in the AT-DBD-gas substitution and RF jet discharge methods were significantly improved compared to the control. On the other hand, in the case of the AT-DBD discharge method, there was no significant difference due to plasma treatment at a similar level to the control.

According to the above results, AT-DBD-gas substitution discharge method was selected as the optimal plasma processing model of the present invention.

Example 2. Comparison of functional ingredient content and antioxidant activity according to plasma treatment conditions

The functional ingredients and antioxidant activity contained in the peanut skin extract were compared and analyzed according to plasma treatment conditions, such as the main types of active species detected near the plasma generation and plasma treatment temperature as confirmed in Experimental Example 2 above.

Specific plasma treatment conditions include the active species being O ₃ or _NO The same procedure was performed.

The results are shown in Tables 3 and 4 below ( p<0.05 ).

As shown in Table 3, the total polyphenol and flavonoid content of the peanut skin increased when treated with O ₃ among the active species generated during plasma treatment, especially when the plasma treatment temperature was 150°C. content was shown. On the other hand, when treated with _NO

As shown in Table 4, it was confirmed that the antioxidant activity according to the type of active species and plasma treatment temperature was excellent in the following order: O ₃ , 150°C > O ₃ , 25°C > NO _x , and 150°C ≥ control. These results were determined to be due to an increase in antioxidant activity due to an increase in the total polyphenol and flavonoid content of peanut outer skin according to plasma treatment conditions.

Example 3. Comparison of enzyme inhibitory activity according to plasma treatment conditions

To examine the possibility of using peanut skin as a cosmetic material, we compared and analyzed the activities of three enzymes (tyrosinase, elastase, and collagenase) related to skin aging according to plasma treatment conditions.

Tyrosinase inhibitory activity

Tyrosinase is an enzyme that produces intracellular melanin and causes pigmentation, and the inhibitory activity of this enzyme was confirmed depending on plasma treatment conditions.

Specifically, the extract was diluted to 1 mg/mL using 0.1 M potassium phosphate buffer (pH 6.8). To 40 μL of the extract, 80 μL of 0.1 M potassium phosphate buffer (pH 6.8), 40 μL of mushroom tyrosinase (100 unit/mL), and 40 μL of 10 mM L-3,4-dihydroxyphenylalanine were added and reacted for 10 minutes. Absorbance was measured at 475 nm. was measured.

Elastase inhibitory activity

Elastase is an enzyme that causes wrinkles by damaging (cutting) elastin molecules within cells, and the inhibitory activity of this enzyme was confirmed depending on plasma treatment conditions.

Specifically, the extract was diluted to 0.1 mg/mL using 50 mM Trizma-base buffer (pH 7.5). To 10 μL of the diluted extract, 70 μL of 50 mM Trizma-base buffer (pH 7.5), 5 μL of porcine pancreatic elastase type IV (0.45 units/mL), and 20 μL of 2 mM N-succinyl-Ala-Ala-Ala-p-nitroanilide. was added and reacted for 30 minutes, and then the absorbance was measured at 405 nm.

Collagenase inhibitory activity

Collagenase, like elastase, is an enzyme that causes wrinkles by damaging (cleaving) collagen molecules within cells, and the inhibitory activity of this enzyme was confirmed depending on plasma treatment conditions.

Specifically, the extract was diluted to 1 mg/mL using 50 mM Trizma-base buffer (pH 7.5). To 40 μL of the diluted extract, 120 μL of 50 mM Trizma-base buffer (pH 7.5), 40 μL of collagenase (50 μg/mL), and 50 mM metalloproteinase-2 were added and reacted for 30 minutes, followed by excitation at 320 nm and emission by spectrofluorescence. Analyzed at 405 nm.

The results are shown in Table 5 below ( p<0.05 ).

As shown in Table 5, the inhibitory activity of each enzyme, tyrosinase, elastase, and collagenase, according to the type of active species and plasma treatment temperature, was the best in the O ₃ , 150°C treatment group, and the inhibitory activity of tyrosinase was higher than that of the control group. This increased by about 20%.

Example 4. Analysis of test results for functional ingredient content and physiological activity

Heatmap analysis

Through Examples 2 and 3, the test results for the content of functional ingredients and physiological activity of peanut skin according to plasma treatment conditions were confirmed through heatmap analysis, and the normalized heatmap was created using MetaboAnalyst 5.0 ( https://www. It was created using the program metaboanalyst.ca ).

Variable Importance Important in Project (VIP) Analysis

In order to confirm the degree of influence involved in the differences between treatments through multivariate analysis, VIP analysis was performed, and items with a VIP score of 1.0 or more confirmed by PLS-DA were judged to be significant variables.

As a result, as shown in Figure 3, the heatmap analysis results confirmed again that all items were superior in the O ₃ , 150°C treatment group, and the NO _x , 150°C treatment group had higher DPPH radical scavenging ability and tyrosinase inhibitory activity than the control group. However, other items tended to be similar to or slightly lower than those in the control group (Figure 3a). In addition, the VIP analysis results confirmed that tyrosinase inhibitory activity and DPPH radical scavenging ability served as major variables indicating differences between treatments (Figure 3b).

In summary, among the types of plasma active species, the total polyphenol and flavonoid content of peanut skin was significantly increased under the conditions of O ₃ and processing temperature of 150°C, and this increase in functional ingredient content was related to the antioxidant activity and antioxidant activity of peanut skin extract. It can be expected to have improved whitening and wrinkle improvement activity. Through this, it was confirmed that the O ₃ , 150°C plasma treatment conditions can be applied to the functional ingredients and physiological activity enhancement technology of peanut skin for use in cosmetics, etc.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. In this regard, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed as including the meaning and scope of the patent claims described below rather than the detailed description above, and all changes or modified forms derived from the equivalent concept thereof are included in the scope of the present invention.

Claims (13)

Preparing peanut shells;
Treating the peanut shell with plasma; and
A method for producing a peanut skin extract with improved functionality, comprising extracting the plasma-treated peanut skin. The method of claim 1, wherein the plasma treatment is a Surface Dielectric Barrier Discharge method. The method of claim 2, wherein the discharge method is an atmospheric pressure dielectric barrier discharge-gas exchange (AT-DBD-gas exchange) method. The method of claim 1, wherein the plasma treatment treats O ₃ as an active species. The method of claim 1, wherein the plasma treatment is performed at a temperature of 140°C to 160°C. The method of claim 1, wherein the extract is extracted with one or more solvents selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, or mixed solvents thereof. The method of claim 1, wherein the functional improvement is an increase in the functional ingredients, antioxidant activity, or enzyme inhibitory activity contained in the outer shell of the peanut. The method of claim 7, wherein the functional ingredients are total polyphenols and flavonoids. The method of claim 7, wherein the enzyme is at least one selected from the group consisting of tyrosinase, elastase, and collagenase. Preparing peanut shells;
Treating the peanut shell with plasma; and
A method of improving peanut skin functionality, comprising extracting the plasma-treated peanut skin. Peanut skin extract whose functionality has been improved by the method of claim 10. A cosmetic composition for skin whitening or wrinkle improvement comprising the peanut skin extract of claim 11. A quasi-drug composition for skin whitening or wrinkle improvement comprising the peanut skin extract of claim 11.