KR20240039764A - ANTIBACTERIAL AND ANTIFUNGAL COMPOSITION COMPRISING Juniperus chinensis extract - Google Patents

Abstract

The present invention relates to an antibacterial and antifungal composition containing an extract of Juniperus chinensis L. Since the juniper extract according to the present invention exhibits antibacterial and antifungal activity against various pathogenic bacteria and fungi, it can prevent, improve, alleviate or alleviate various diseases and conditions caused by bacteria and fungi whose growth is inhibited by the juniper extract of the present invention. For the purpose of treatment, it can be widely used in various industrial fields such as food, health functional food, pharmacy, cosmetics, quasi-drugs, feed additives, and cleaning and disinfection compositions.

Description

Antibacterial and antifungal composition containing juniper extract {ANTIBACTERIAL AND ANTIFUNGAL COMPOSITION COMPRISING Juniperus　chinensis extract}

The present invention relates to an antibacterial and antifungal composition containing an extract of Juniperus chinensis L.

Pathogenic microorganisms cause several infectious diseases in humans and livestock. For example, Staphylococcus aureus , a Gram-positive bacterium, is one of the causative bacteria that invades the human body and causes skin infections such as abscesses and wound infections, osteoarthritis, bacteremia, pneumonia, toxic shock syndrome, and food poisoning. Pyogenic bacteria are causative bacteria that cause infectious diseases in animals, and in particular, together with Escherichia coli , they cause acute mastitis in cattle. Escherichia coli is a gram-negative bacterium and is generally present in the intestinal tract of humans and livestock, but is also detected in the urinary tract and respiratory tract in animals. E coli O157:H7, a representative pathogenic E. coli, is enterohemorrhagic Escherichia coli and causes food poisoning, hemolytic uremic syndrome, hemolytic colitis, and kidney dysfunction in humans. do. Additionally, it has a very low ID _{of 50} , so the infection rate is very high.

In general, direct or indirect damage caused by pathogenic microorganisms is causing many economic, environmental, and medical problems. In particular, losses due to spoilage during the food distribution process in the food industry, harm to the human body and environmental pollution due to the use of excessive amounts of chemical pesticides on crops in agriculture, and the emergence of antibiotic-resistant strains due to misuse and abuse of antibiotics have already become a problem in society. It is becoming a chronic problem across the board. In this reality, natural bioactive substances isolated from plants, etc. exhibit antibiotic activity against a wide range of pathogenic bacteria, fungi, etc., and can be used in an environmentally friendly manner, so the development of new alternative substances is urgently needed.

Juniperus chinensis L. is an evergreen tree of the gymnosperm conifer subclass Conifer family Cupressaceae and grows naturally in Korea, Japan, China, and Mongolia. It was named juniper because its wood has been used as incense for a long time. It grew in abundance in deep mountainous areas, especially on Ulleungdo Island, but most of it has disappeared and is commonly planted for ornamental purposes. Wood is widely used in making pencils, and is known to be used in other materials such as sculpture, furniture, and decoration. However, studies on applying juniper to antibiotics and antibacterial agents have not yet been widely reported.

While trying to find a new natural substance that exhibits antibacterial and antifungal activity, the present inventors confirmed that an extract of Juniperus chinensis L. exhibits antibacterial activity against various pathogenic bacteria and fungi and completed the present invention. Therefore, the purpose of the present invention is to provide an antibacterial and antifungal composition containing juniper extract.

In order to achieve the above object, the present invention provides an antibacterial and antifungal composition containing an extract of Juniperus chinensis L..

The present invention also provides a food composition containing the above composition.

Additionally, the present invention provides a health functional food composition containing the above composition.

The present invention also provides a pharmaceutical composition containing the above composition.

Additionally, the present invention provides a cosmetic composition containing the composition.

Additionally, the present invention provides a quasi-drug composition containing the above composition.

The present invention also provides a feed additive composition containing the above composition.

The present invention also provides a composition for cleaning and disinfection containing the composition.

Since the juniper extract according to the present invention exhibits antibacterial and antifungal activity against various pathogenic bacteria and fungi, it can prevent, improve, alleviate or alleviate various diseases and conditions caused by bacteria and fungi whose growth is inhibited by the juniper extract of the present invention. For the purpose of treatment, it can be widely used in various industrial fields such as food, health functional food, pharmacy, cosmetics, quasi-drugs, feed additives, and cleaning and disinfection compositions.

The present invention relates to an antibacterial and antifungal composition containing juniper extract. The juniper extract of the present invention is excellent in inhibiting the growth of various bacteria and fungi.

Hereinafter, the present invention will be described in detail.

The present invention relates to an antibacterial and antifungal composition containing juniper extract.

In the present invention, the juniper extract refers to an extract obtained from the juniper tree as a raw material, and may include all parts of the juniper tree without limitation as long as the antibacterial and antifungal purpose of the present invention can be achieved, such as whole plants, leaves, It may be an extract obtained from one or more parts selected from the group consisting of flowers, branches, fruits, roots, stems and seeds, and in a preferred embodiment of the present invention, a leaf extract of juniper is used.

The extract of the juniper tree may be an extract extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and may be extracted with water, methanol, ethanol, ethyl acetate, acetone, ether, chloroform, benzene, hexane, dichloromethane and these. It may be extracted with one or more solvents selected from the group consisting of mixed solvents.

Preferably, the extraction solvent may be an extract of a lower alcohol having 1 to 4 carbon atoms, and more preferably, it may be an ethanol extract. When the juniper extract of the present invention is an ethanol extract, the ethanol extract may be 50 to 99% (v/v) ethanol extract, preferably 60 to 80% (v/v), more preferably 65 to 75%. It may be % (v/v) ethanol extract.

The term 'extract' used in this specification has the meaning commonly used in the art as a crude extract, as described above, but in a broad sense also includes fractions obtained by additional fractionation of the extract. In other words, plant extracts include not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process. For example, fractions obtained by passing the extract through an ultrafiltration membrane with a certain molecular weight cut-off value, separation by various chromatographs (designed for separation according to size, charge, hydrophobicity, or affinity), etc. Fractions obtained through various purification methods are also included in the plant extract of the present invention.

The extract used in the present invention can be prepared in powder form by additional processes such as reduced pressure distillation and freeze drying or spray drying.

The juniper extract of the present invention exhibits antibacterial and antifungal activity against various bacteria and fungi, and may be characterized as inhibiting the growth of bacteria and fungi. Preferably, the antibacterial and antifungal composition of the present invention is Escherichia coli, Salmonella enterica, Staphylococcus aureus, Streptococcus mutans, Acinetoboxer virii, Bordetella pertussis, and Klebsiella pneumoniae. It can be characterized by exhibiting antibacterial and antifungal activity against two or more types of bacteria selected from the group consisting of Moraxella catarrhalis, Streptococcus pneumoniae, Aspergillus fumigatus, and Streptococcus pyogenes. there is.

Escherichia coli ( E. coli ) is a gram-negative bacterium that causes food poisoning.

Salmonella enteica , a member of the Salmonella genus, is not only a common cause of food poisoning, but also a cause of typhoid fever and paratyphoid fever. Salmonella enterica of the present invention may be Salmonella enterica Serovar Enteritidis.

Staphylococcus aureus is also a representative food poisoning-causing bacteria and is known as a representative Gram-positive pathogenic microorganism that causes purulent diseases in humans.

Streptococcus mutans is one of the oral bacteria and is a representative cariogenic bacteria that causes oral cavities.

Acinetobacter baylyi is Acinetobacter sp. Also called ADP1, it is a Gram-negative fungus found in soil. Natural transformation is active, allowing expression of various foreign genes such as antibiotic resistance genes and essential metabolic genes, and can cause lung diseases in children or people with weakened immunity due to diseases such as acute leukemia, diabetes, and cancer. It is classified as an opportunistic infection.

Bordetella pertussis is a Gram-negative bacterium of the Bordetella genus and is transmitted through pertussis toxin, hemagglutinin, adenyl cyclase, and bacterial lipopolysaccharide (LPS). Because it causes whooping cough, it is known as the causative agent of pertussis (whooping cough), a human respiratory disease.

Klebsiella pneumoniae is a Gram-negative, genus of Enterobacteriaceae that was discovered as the cause of community pneumonia over 100 years ago, and has recently been known to be the main cause of urinary tract infections, sepsis, pneumonia, and abdominal cold infections. In particular, Klebsiella pneumoniae is a representative bacteria that causes pneumonia, ranking fourth among the bacteria that cause pneumonia.

Moraxella catarrhgalis is an aerobic gram-negative diplococcus that mainly causes otitis media and sinusitis in infants and children, and is a major pathogen that worsens chronic obstructive pulmonary disease (COPD) in adults. It is known. In addition, serious infections such as pneumonia, endocarditis, meningitis, and sepsis have been reported, and it is known to be a risk factor for causing subacute or chronic sinusitis in patients with bronchial asthma or respiratory allergies, making it a causative agent of various respiratory diseases. are classified.

Streptococcus pneumoniae is a pneumococcus, a gram-positive bacterium, and is known to be the main cause of pneumonia. Among bacterial pneumonias, 60-70% are also bacteria that cause Streptococcus pneumoniae, osteomyelitis, arthritis, and peritonitis. In particular, Streptococcus pneumoniae is the most common cause of bacteremia, pneumonia, meningitis, and otitis media in young children.

Aspergillus fumigatus is a fungus belonging to the Ascomycetes that causes infections without colony formation and is known to infiltrate the human respiratory tract and cause aspergillosis.

Streptococcus pyogenes is a Gram-positive coccus that forms part of the flora that inhabits the skin. Its main habitat is the human throat and skin, and the respiratory tract is also infected through aerosols. Streptococcus pyogenes is also known to cause purulent diseases such as pharyngitis and cellulitis, and non-purulent diseases such as rheumatic fever and acute pyelonephritis.

As described above, the juniper extract of the present invention exhibits a broad growth inhibitory effect on bacteria that cause food poisoning, bacteria that cause cavities, bacteria that cause various respiratory diseases such as pneumonia, and bacteria that cause purulent diseases, so it can be used for various purposes depending on the purpose. It can be used for antibacterial and antifungal purposes.

Therefore, the present invention can be used in a variety of ways as food, health functional food composition, pharmaceutical composition, cosmetic composition, quasi-drug composition, feed additive composition, and cleaning and disinfection composition containing the above antibacterial and antifungal composition.

When the composition of the present invention is used as a food or health functional food composition, Escherichia coli, Salmonella enterica, Staphylococcus aureus, Streptococcus mutans, Acinetoboxer virii, Bordetella pertussis, Various diseases and conditions caused by two or more types of bacteria selected from the group consisting of Klebsiella pneumoniae, Moraxella catarrhalis, Streptococcus pneumoniae, Aspergillus fumigatus, and Streptococcus pyogenes. It can be used for prevention or improvement purposes.

Food compositions according to the present invention include all types of functional foods, nutritional supplements, health foods, health supplements, and food additives. The food composition of the above type may be formulated in any form selected from the group consisting of powder, tablet, capsule, pill, and liquid according to a conventional method known in the art, but is not limited thereto. It can be manufactured in various forms using methods known in the art.

For example, as a health food, the juniper extract of the present invention can be granulated, encapsulated, and powdered and consumed, or prepared in the form of tea, juice, and drink. In addition, the juniper extract of the present invention can be prepared in the form of a composition by mixing it with known substances or active ingredients known to have antibacterial and antifungal effects.

In addition, functional foods include beverages (including alcoholic beverages), fruits and their processed foods (e.g. canned fruit, bottled foods, jam, marmalades, etc.), fish, meat and their processed foods (e.g. ham, sausage corn beef, etc.) , bread and noodles (e.g. udon, buckwheat noodles, ramen, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, taffy, dairy products (e.g. butter, cheese, etc.), edible vegetable oil, margarine, vegetable protein, retort. It can be manufactured by adding the juniper extract of the present invention to food, frozen food, various seasonings (e.g. soybean paste, soy sauce, sauce, etc.).

In addition, the food composition of the present invention may contain common food additives, and its suitability as a "food additive" is determined in accordance with the general provisions of the food additive code and general test methods approved by the Ministry of Food and Drug Safety, unless otherwise specified. It is determined based on the specifications and standards for the relevant item. Items listed in the "Food Additives Code" include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid; natural additives such as subchromic pigment, licorice extract, crystalline cellulose, high-liquid pigment, and guar gum; Examples include mixed preparations such as sodium L-glutamate preparations, noodle additive alkaline preparations, preservative preparations, and tar coloring preparations.

In the food composition of the present invention, the juniper extract may be preferably included in an amount of 0.00001 to 50% by weight relative to the food composition. If the content is less than 0.00001% by weight, the effect is insignificant, and if it exceeds 50% by weight, the increase in effect compared to the amount used is minimal, making it uneconomical.

Additionally, in order to use the juniper extract of the present invention in the form of a food additive, it can be manufactured and used in the form of tablets, capsules, powders, granules, liquid, pills, etc.

When the composition of the present invention is manufactured into a beverage, it may contain various flavoring agents or natural carbohydrates as additional ingredients, like conventional beverages. The above-mentioned natural carbohydrates may include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, natural sweeteners such as dextrin and cyclodextrin, and synthetic sweeteners such as saccharin and aspartame. The proportion of natural carbohydrates is generally about 0.01 to 10 g, preferably about 0.01 to 0.1 g, per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention contains various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, It may include carbonating agents used in carbonated drinks. Additionally, the composition of the present invention may include pulp for the production of natural fruit juice, fruit juice beverages, and vegetable beverages. These ingredients can be used independently or in combination. The ratio of these additives is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

In the present invention, “health supplement” or “health functional food” means food manufactured and processed using raw materials or ingredients with functionality useful to the human body in accordance with the Health Functional Food Act. , “Functional” means ingestion for the purpose of controlling nutrients for the structure and function of the human body or obtaining useful effects for health purposes such as physiological effects.

The composition of the present invention may be a pharmaceutical composition. When the composition of the present invention is used as a pharmaceutical composition, Escherichia coli, Salmonella enterica, Staphylococcus aureus, Streptococcus mutans, Acinetoboxer virii, Bordetella pertussis, and Klebsiella Prevents and improves various diseases and conditions caused by two or more types of bacteria selected from the group consisting of Pneumoniae pneumoniae, Moraxella catarrhalis, Streptococcus pneumoniae, Aspergillus fumigatus, and Streptococcus pyogenes. , or can be used for therapeutic purposes.

In particular, the juniper extract of the present invention is used against human beings such as Acinectobacter birii, Bordetella pertussis, Klebsiella pneumoniae, Moraxella catarrhalis, Streptococcus pneumoniae, and Aspergillus fumigatus. Since it exhibits growth inhibitory activity against bacteria known to cause diseases in the respiratory tract, the pharmaceutical composition may be used to prevent or treat respiratory diseases, and is preferably Acinetobacter virii, Bordetella pertussis, It may be used for the prevention or treatment of respiratory diseases caused by one or more types of bacteria selected from the group consisting of Klebsiella pneumoniae, Moraxella catarrhalis, Streptococcus pneumoniae, and Aspergillus fumigatus.

The respiratory disease is 1 selected from the group consisting of Acinectobacter birii, Bordetella pertussis, Klebsiella pneumoniae, Moraxella catarrhalis, Streptococcus pneumoniae, and Aspergillus fumigatus. Diseases caused by more than one species of bacteria may be included without limitation, but include, for example, whooping cough, bronchitis, laryngitis, pneumonia, emphysema, chronic obstructive lung disease, It may be one or more types selected from the group consisting of asthma and aspergillosis.

The pharmaceutical composition of the present invention can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, and sterile injection solutions according to conventional methods. there is. Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations include the juniper extract of the present invention with at least one or more excipients, such as starch, calcium carbonate, sucrose or lactose. It is prepared by mixing gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, oral solutions, emulsions, syrups, etc. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. . Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao, laurin, glycerogeratin, etc. can be used.

The dosage of the pharmaceutical composition of the present invention will vary depending on the age, gender, and weight of the subject to be treated, the specific disease or pathological state to be treated, the severity of the disease or pathological state, the route of administration, and the judgment of the prescriber. Dosage determinations based on these factors are within the level of one skilled in the art, and dosages generally range from 0.01 mg/kg/day to approximately 2000 mg/kg/day. A more preferred dosage is 0.1 mg/kg/day to 1000 mg/kg/day. Administration may be administered once a day, or may be administered several times. The above dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, livestock, and humans through various routes. In the present invention, “administration” means providing a given composition of the present invention to an individual by any suitable method. The pharmaceutical composition of the present invention can be administered through various routes, including oral or parenteral.

The pharmaceutical composition of the present invention may further include substances known to be effective in antibacterial and antifungal effects in addition to the juniper extract of the present invention.

Additionally, the present invention relates to a cosmetic composition containing the composition of the present invention.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to juniper extract, for example, conventional auxiliaries and carriers such as stabilizers, solubilizers, vitamins, pigments, and fragrances. Includes.

The cosmetic composition of the present invention can be manufactured in any formulation commonly manufactured in the art, and examples include emulsions, creams, lotions, packs, foundations, lotions, beauty essences, hair cosmetics, etc.

Specifically, the cosmetic composition of the present invention includes skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, It includes formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions, and body cleansers.

More preferably, it is a formulation of hair tonic, hair cream, hair lotion, hair shampoo, hair rinse, hair conditioner, hair spray, hair aerosol, pomade, powder gel, hair pack, hair treatment, eyebrow hair growth agent, eyelash growth medicine or eyelash nutrition. However, it is not limited to this.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide. Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester can be used.

In the present invention, when using the juniper extract as a cosmetic composition, the extract may be added in an amount of 0.001 to 50.0% by weight based on the total weight of the cosmetic composition, and is preferably added in an amount of 0.005 to 10.0% by weight. You can.

Additionally, the cosmetic composition of the present invention can be used for pets depending on its formulation. For example, it can be manufactured in various forms such as solution, gel, emulsion, oil, wax, and aerosol, such as pet shampoo and pet rinse, and is a neutral detergent that is less irritating to the pet's skin and has excellent moisturizing properties. It can be manufactured by adding.

Additionally, the present invention can be used in the form of a quasi-drug composition.

In the present invention, the "quasi-drugs" are fibers, rubber products or similar products used for the purpose of treating, alleviating, treating or preventing diseases in humans or animals, and have a weak effect on the human body or do not act directly on the human body, It refers to items that are not instruments or machines, items similar to them, or preparations used for sterilization, insecticide, and similar purposes to prevent infection. Among articles used for the purpose of diagnosing, treating, alleviating, treating, or preventing diseases in humans or animals that are not instruments, machines, or devices, and among articles used for the purpose of having a pharmacological effect on the structure and function of humans or animals, instruments , refers to goods excluding those that are not machines or devices, and also includes external skin preparations and personal hygiene products.

The composition of the present invention can be used against Escherichia coli, Salmonella enterica, Staphylococcus aureus, Streptococcus mutans, Acinetoboxer virii, Bordetella pertussis, Klebsiella pneumoniae, and Moraxella catarrhal. To be included in quasi-drugs for the purpose of preventing or improving various conditions and symptoms caused by two or more types of bacteria selected from the group consisting of Streptococcus pneumoniae, Aspergillus fumigatus, and Streptococcus pyogenes. In this case, the composition can be used as is or together with other quasi-drug ingredients, and can be used appropriately according to conventional methods. The mixing amount of the active ingredient can be appropriately determined depending on the purpose of use.

The quasi-drug of the present invention is not particularly limited thereto, but may be manufactured and used in the form of, for example, cream, lotion, aerosol, shampoo, gel, or pack.

In the case of creams, ointments, shampoos, gels or packs, white petrolatum, yellow petrolatum, lanolin, bleached beeswax, cetanol, stearyl alcohol, stearic acid, hydrogenated oil, gelling hydrocarbons, polyethylene glycol, liquid paraffin, squalane, etc. Mechanism of; Solvents and solubilizers such as oleic acid, isopropyl myrstate, glycerin triisooctanoate, crotamiton, diethyl sebacate, diisopropyl adipate, hexyl laurate, fatty acids, fatty acid esters, fatty alcohols, and vegetable oils; Antioxidants such as tocopherol derivatives, L-ascorbic acid, dibutylhydroxytoluene, and butylhydroxyanisole; Preservatives such as parahydroxybenzoic acid ester; Moisturizers such as glycerin, propylene glycol, and sodium hyaluronate; Surfactants such as polyoxyethylene derivatives, glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, and lecithin; There are thickeners such as carboxyvinyl polymer, xanthan gum, carboxymethyl cellulose, carboxymethyl cellulose sodium salt, hydroxypropyl cellulose, and hydroxypropyl methyl cellulose.

In the case of aerosol preparations, white petrolatum, yellow petrolatum, lanolin, bleached beeswax, cetanol, stearyl alcohol, stearic acid, etc. are used in the preparation of ointments, creams, gels, suspensions, emulsions, solutions and lotions. Bases such as hydrogenated oil, gelled hydrocarbon, polyethylene glycol, liquid paraffin, and squalane; Oleic acid, isopropyl myrstate, diisopropyl adipate, isopropyl sebacate, glycerin triisooctanoate, crotamiton, diethyl sebacate, hexyl laurate, fatty acids, fatty acid esters, fatty alcohols, vegetable oils, etc. Solvents and dissolution aids; Antioxidants such as tocopherol derivatives, L-ascorbic acid, dibutylhydroxytoluene, and butylhydroxyanisole; Preservatives such as parahydroxybenzoic acid ester; Moisturizers such as glycerin, propylene glycol, and sodium hyaluronate; Surfactants such as polyoxyethylene derivatives, glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, and lecithin; Thickeners such as carboxyvinyl polymer, xanthan gum, carboxymethylcellulose, carboxymethylcellulose sodium salt, hydroxypropylcellulose, and hydroxypropylmethylcellulose; In addition, various stabilizers, buffers, coagulants, suspending agents, emulsifiers, fragrances, preservatives, solubilizers, and other appropriate additives can be added. In addition, stabilizers, preservatives, absorption accelerators, pH adjusters, and other appropriate additives can be added as needed.

In addition, the present invention relates to a feed additive composition or a cleaning and disinfection composition for use in industrial fields.

The term "feed" of the present invention refers to any natural or artificial diet, meal, etc., or an ingredient of the meal, which is eaten by animals, and the composition for adding feed for the purpose of antibacterial and antifungal effects according to the present invention. Feed containing active ingredients can be manufactured from various types of feed known in the art, and may preferably include concentrated feed, roughage and/or special feed, but is not limited thereto.

In the present invention, the term “composition for adding feed” may mean “feed additive”, which means alleviating disease symptoms in animals, supplementing nutrients and preventing weight loss, improving digestibility of fiber in feed, improving oil quality, and breeding. It contains substances added to feed for various effects, such as preventing disability, improving conception rate, and preventing high temperature stress in the summer.

The composition for adding feed of the present invention corresponds to supplementary feed under the Feed Management Act, and includes mineral preparations such as sodium bicarbonate, bentonite, magnesium oxide, and complex mineral substances, and trace mineral preparations such as zinc, copper, cobalt, and selenium. , vitamins such as kerotene, vitamins A D, E, nicotinic acid, and vitamin B complex, protective amino acids such as methionine and lysine, protective fatty acids such as fatty acid calcium salts, probiotics (lactic acid bacteria), yeast cultures, fungal fermentation products, etc. Probiotics, yeast, etc. may be additionally included.

The composition for adding feed according to the present invention can be prepared by adding the juniper extract of the present invention in an appropriate effective concentration range according to various feed production methods known in the art.

The feed additive according to the present invention can be applied without limitation to any object intended for the antibacterial and antifungal effect according to the present invention. For example, it can be applied to any entity, including non-human animals such as monkeys, dogs, cats, rabbits, guinea pigs, rats, mice, cows, sheep, pigs, goats, birds, and fish.

The present invention also relates to a disinfection and cleaning composition comprising the composition of the present invention.

The composition can be used as a disinfectant and detergent, and the term "disinfectant" in the present invention refers to a drug for the purpose of sterilizing pathogenic bacteria, and the term "detergent" in the present invention refers to the purpose of cleaning. refers to a drug used for

The disinfectant and detergent of the present invention can be easily prepared according to known methods, including one or more excipients or additives commonly used in the art. In addition, in addition to the juniper extract of the present invention, it may further include other known substances with antibacterial and antifungal activity or substances that can impart other functionality.

The contents of the present invention described above are applied equally to each other unless they contradict each other, and implementation by a person skilled in the art with appropriate changes is also included in the scope of the present invention.

Hereinafter, the present invention will be described in detail through examples, but the scope of the present invention is not limited to the following examples.

Example 1. Preparation of juniper extract

10 times the weight of 70% (v/v) ethanol was added to 100 g of dried powder of juniper leaves, and the extract was extracted by applying ultrasound while stirring three times for 24 hours at room temperature, and then filtered through filter paper. The obtained 70% ethanol filtrate was first concentrated under reduced pressure at room temperature using a rotary vacuum concentrator, and then lyophilized to remove as much of the remaining solvent as possible to obtain an extract. The above extract was purchased and used from the Wildlife Natural Products Bank of the National Institute of Biological Resources.

Example 2. Confirmation of antibacterial activity of juniper extract

An experiment was performed to confirm the antibacterial activity of the juniper extract prepared in Example 1. The antibacterial activity test was performed using the pathogenic bacteria shown in Table 1 below distributed from NCCP or ATCC. Specifically, using a medium suitable for the growth of each pathogenic bacteria in Table 1, the test was performed according to the Disc diffusion assay of the Antimicrobial disk susceptibility test, which is a guideline of CLSI (Clinical & Laboratory Standard Institute).

pathogen Disclosure strain
code Positive control group badge Escherichia coli KCTC 2617 Ampicillin 2.5mg/mL Nutrient agar plate medium Salmonella enterica Serovar Enteritidis NCCP 14546 Gentamicin 2.5mg/mL Tryptic soy agar plate medium Streptococcus mutans KCTC 3065 Kanamycin 50mg/mL Brain Heart Infusion agar plate medium Staphylococcus aureus NCCP 14560 Gentamicin 50mg/mL Nutrient agar plate medium Acinetobacter baylyi ATCC 33305 Gentamicin 2.5mg/mL Brain Heart Infusion agar plate medium Klebsiella pneumoniae NCCP 16052 Gentamicin 2.5mg/mL Nutrient agar plate medium Bodetella pertussis NCCP13671 Gentamicin 2.5mg/mL Bordet Gengou Agar plat medium Moraxella catarrhalis ATCC 43628 Gentamicin 2.5mg/mL Brain Heart Infusion agar plate medium Streptococcus pyrogenes NCCP 14783 Gentamicin 2.5mg/mL Brain Heart Infusion agar plate medium Streptococcus pneumoniae NCCP14774 Gentamicin 2.5mg/mL 5% sheep blood agar plate medium Aspergillus fumigatus NCCP 32662 Hygromycin50mg/mL Potato dextrose agar plate medium Candida albicans NCCP 31077 Hygromycin
10mg/mL Sabouraud dextrose agar plate medium

Each pathogenic bacteria was plated on each agar plate medium at a concentration of 1 ^to 2 20㎕ of the sample (sample concentration 200,000ppm) was dropped and absorbed. Afterwards, the culture was incubated at 37°C for 1 day and the antibacterial activity was analyzed by measuring the size of the growth inhibition zone (clear zone, mm) around the disk. The positive control group (PC) used antibiotics that act on each pathogen (Table 1), and the negative control group (NC) used 70% ethanol used when diluting the sample. The results were negative in terms of the size of growth inhibition rings in the sample treatment group. (This is the value obtained by subtracting the size of the growth inhibitory tube in the control group), the average value was obtained by repeating the experiment three times. The results are shown in Table 2 below.

pathogen PC NC sample Escherichia coli 12.0mm - 10.0mm Salmonella enterica Serovar Enteritidis 16.0mm - 9.6mm Streptococcus mutans 16.0mm - 12.0mm Staphylococcus aureus 14.0mm - 11.7mm Acinetobacter baylyi 12mm - 9mm Klebsiella pneumoniae 12mm - 9mm Bodetella pertussis 16mm - 15mm Moraxella catarrhalis 16mm - 9mm Streptococcus pyogenes 12mm - 12mm Streptococcus pneumoniae 12mm - 10.3mm Aspergillus fumigatus 18.0mm - 13mm Candida albicans 12.0 mm - -

As confirmed in Table 2 above, the juniper extract is Escherichia coli, Salmonella enterica, Streptococcus mutans, Staphylococcus aureus, Acinetoboxer virii, Bordetella pertussis, and Klebsiella new. It showed antibacterial activity against Monia, Moraxella catarrhalis, Streptococcus pyogenes, Streptococcus pneumoniae, and Aspergillus fumigatus.

Claims (15)

An antibacterial and antifungal composition comprising an extract of Juniperus chinensis L.
The antibacterial and antifungal composition according to claim 1, wherein the juniper extract is an extract obtained from one or more parts selected from the group consisting of the whole plant, leaves, flowers, branches, fruits, roots, stems and seeds of the juniper tree.
The antibacterial and antifungal composition according to claim 1, wherein the juniper extract is an extract extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.
The method of claim 1, wherein the juniper extract is extracted with one or more solvents selected from the group consisting of water, methanol, ethanol, ethyl acetate, acetone, ether, chloroform, benzene, hexane, dichloromethane, and mixed solvents thereof. , Composition for antibacterial and antifungal use.
The composition for antibacterial and antifungal use according to claim 4, wherein the ethanol is 50 to 99% (v/v) ethanol.
The method of claim 1, wherein the composition is Escherichia coli, Salmonella enterica, Staphylococcus aureus, Streptococcus mutans, Acinetoboxer virii, Bordetella pertussis, Klebsiella pneumoniae, A composition for antibacterial and antifungal treatment against two or more types of bacteria selected from the group consisting of Moraxella catarrhalis, Streptococcus pneumoniae, Aspergillus fumigatus and Streptococcus pyogenes.
A food composition comprising the composition of any one of claims 1 to 6.
A health functional food composition comprising the composition of any one of claims 1 to 6.
A pharmaceutical composition comprising the composition of any one of claims 1 to 6.
The pharmaceutical composition of claim 9, wherein the pharmaceutical composition is for preventing or treating respiratory diseases.
The method of claim 10, wherein the respiratory diseases include whooping cough, bronchitis, laryngitis, pneumonia, emphysema, chronic obstructive lung disease, asthma and aspergillus. A pharmaceutical composition, which is at least one selected from the group consisting of gillosis.
A cosmetic composition comprising the composition of any one of claims 1 to 6.
A quasi-drug composition comprising the composition of any one of claims 1 to 6.
A feed additive composition comprising the composition of any one of claims 1 to 6.
Paragraph 1 A cleaning and disinfecting composition comprising the composition of any one of claims 6 to 6.