KR20230174775A - Manufacturing Device of Petides and Amino acids - Google Patents

Abstract

The peptide and amino acid production device of the present invention is a production device for producing low-molecular peptides, liquid amino acids, or amino acid powder by hydrolyzing high molecular weight proteins present in silkworms or insects, etc., and is a production device that dramatically reduces the production time and production rate of low-molecular peptides and amino acids. It is about a high-efficiency, high-purity peptide and amino acid production device that can be improved.
According to the present invention, the production time of peptides and amino acids is significantly shortened compared to the previous one, and the production rate of high-purity amino acids is improved, as well as the production cost and production rate of high-purity amino acids, so that high-purity amino acids can be used in various fields such as food, cosmetics, and pharmaceuticals. It can be applied.

Description

{Manufacturing Device of Petides and Amino acids}

The present invention is a manufacturing device for producing low-molecular peptides, liquid amino acids, or amino acid powder by hydrolyzing high-molecular proteins present in silkworms or insects, etc., and is a high-efficiency/high-purity peptide that can dramatically increase the production time and production rate of low-molecular peptides and amino acids. and amino acid production equipment.

Recently, silkworms and insects have emerged as protein sources that replace livestock/livestock protein, and are being commercialized and released on the market as protein foods, and the related industry is rapidly growing.

In particular, silkworm cocoons are made of sericin and fibroin proteins, and development of these proteins to use them as materials for functional food and beverages or as medical materials has recently been actively underway.

However, the technologies so far have either used the silk protein collected from silkworm cocoons in its original form without undergoing any special processing, or used a method of purifying the dissolved fibroin aqueous solution mostly through dialysis, so the production yield during mass production has been reduced. This was uneconomical, and due to the fibrous structural characteristics of silk protein and the arrangement of amino acids, decomposition in the human body was delayed or difficult to decompose, resulting in a decrease in absorption rate and lower value as a functional/medical material. There was this.

According to the prior art, techniques for producing cocoon/insect peptides or amino acids include chemical decomposition methods such as acid hydrolysis and neutral salt decomposition and biological enzymatic decomposition methods.

Although the chemical decomposition method is easily decomposed and has a high yield, it causes water pollution during the manufacturing process and has the problem of not being able to produce functional peptides because even amino acids other than peptides are decomposed during the hydrolysis step. In addition, the enzymatic decomposition method had a disadvantage in that although the process was simple, the decomposition rate was low, resulting in low yield.

As a result, the quality level of related products using silkworms/insects is low, as silkworms or insects are sold in powder form by simply drying and pulverizing them, or the adult insects are sold in dried form, which not only causes discomfort as edible but does not improve the taste or texture. , low-molecular-weight peptide or amino acid production technology using silkworms or insects also had the disadvantage of being uneconomical as the enzyme reaction time was very long, up to 24 to 72 hours, and the amino acid production rate was low at 40-50%, as well as being expensive and having limited uses.

Meanwhile, in the case of silkworms, depending on the part, the cocoon part has a molecular weight of up to 300,000 Da, and the other parts have a molecular weight of around 100,000 Da. In order to decompose proteins with different molecular weights, different enzymes, input ratios, reaction times, temperature, and pH are required. etc. must be applied differently, but in reality, it is bound to be unproductive and uneconomical, and the existing technology does not suggest detailed technologies but only comprehensive manufacturing methods, and the amino acid production rate is very low and the production time is also considerable through specific enzymatic decomposition alone. It was almost impossible to produce high-efficiency, high-purity amino acids using the previous method.

Therefore, since the proteolytic enzyme reaction reacts very sensitively to the size of protein particles, temperature, pH concentration, etc., optimal conditions for the enzyme reaction can be provided in order to produce high-efficiency and high-purity amino acids through the proteolytic enzyme reaction. Manufacturing equipment is essential.

In other words, high efficiency is achieved through ultrafineization of protein particles for high-efficiency amino acid production and setting of temperature management, PH management, type of enzyme, enzyme input ratio, reaction time, and stirring method to create the best environment for enzymatic decomposition. There is a need for a manufacturing device that can produce peptides and amino acids.

The present invention was devised to solve the problems of the prior art described above, including ultrafine atomization of silkworm or insect protein particles, heating and maintaining temperature to create the best environment for enzymatic decomposition, PH management, types of enzymes, The purpose is to provide a manufacturing device that can decompose and produce high molecular weight proteins of silkworms or insects into low molecular weight peptides or amino acids at a high production rate within a short period of time by setting the stirring method, sterilization method, reaction time, etc.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those skilled in the art from the description of the present invention. .

In order to achieve the above object, the present invention includes a protein decomposition tank (100) that stores a protein solution and is equipped with an enzyme injection device (20) capable of introducing an enzyme that decomposes the protein solution into peptides or amino acids; A side stirrer (50) installed on the side of the protein digestion tank to rotate and agitate the protein solution; An upper stirrer (21) installed at the top of the protein digestion tank (100), which rotates the protein solution by rotating at a predetermined angle with the side stirrer (21); Temperature maintenance means for maintaining the temperature of the protein solution in the protein decomposition tank (100) at a constant level; An ultrasonic generator (30) that applies ultrasonic waves to the protein solution; and a control unit 19 that controls the operation of the temperature maintenance means, the first stirrer 50, the second stirrer 21, and the ultrasonic generator 30.

In the present invention, the ultrasonic generator 30 includes an ultrasonic reactor 41 into which a protein solution is input and which discharges the input protein solution into a protein decomposition tank; A cooling device 40 surrounding the ultrasonic reactor and into which cooling water flows in and out; An ultrasonic vibrator (32) surrounding a portion of the side of the cooling device; and an ultrasonic generator cover 42 that covers the ultrasonic reactor, cooling device, and ultrasonic vibrator.

In the present invention, the protein solution introduced into the ultrasonic reactor (41) is a protein solution that has passed through a wet grinder (92) or a protein solution stored in the protein decomposition tank (100) by the first circulation motor (61). It is desirable to put in.

In the present invention, the ultrasonic generator 30 preferably irradiates the protein solution with ultrasonic waves having a frequency ranging from 20 KH to 40 KH for a certain period of time.

In the present invention, the ultrasonic generator 30 preferably irradiates ultrasonic waves with a frequency of 20 to 40 kHz in order to inject enzymes into the protein decomposition tank and stop the activity of the injected enzymes after a predetermined period of time.

In the present invention, the protein digestion tank 100 further includes sensors 11, 12, 14, 15, 16, and 17 that measure water level, temperature, pH, protein concentration, amino acid concentration, and ultrasound, It is desirable to control the operation by the control unit 19.

In the present invention, the temperature maintenance means supplies hot water to the hot water path 80 formed along the outer peripheral surface of the protein decomposition tank through the boiler 93, and heats the protein to the instantaneous heater 70 mounted on the side of the protein decomposition tank. It is desirable to supply the solution in a circulatory manner to raise and maintain the protein solution at 55°C to 60°C.

In the present invention, the protein solution is continuously supplied to the instantaneous heater (70) through the second circulation motor (62) located at the bottom of the side of the protein digestion tank, and the protein solution with an increased temperature is fed back into the protein digestion tank. It is desirable.

In the present invention, the protein digestion tank 100 maintains a temperature of 55-60°C and a pH of 6.0-8.0 while stirring the stored protein solution at a speed of 60-100 rpm by a first stirrer and a second stirrer. It is desirable.

In the present invention, it is preferable that the side stirrer 50 is installed horizontally in the protein digestion tank, and the stirring propeller 51 rotates and agitates the protein solution in the vertical direction.

In the present invention, the upper stirrer 21 preferably rotates and stirs the protein solution at an angle of 45 degrees with the side stirrer 50.

In the present invention, the protein digestion tank 100 preferably consists of at least two protein digestion tanks connected to each other to sequentially decompose proteins.

In the present invention, the enzyme preferably includes Alcarase as an endo type enzyme and Flavozyme as an Exo type enzyme.

In the present invention, the protein solution is preferably formed by dry grinding silkworm protein powder, insect protein powder, or protein powder mixed with silkworm and insect, followed by wet grinding by adding water.

According to the peptide and amino acid production device of the present invention, when ultrasonic pulverization is performed while passing a mixed solution of protein particles flowing from a storage tank or protein decomposition tank through an ultrasonic generator, the high molecular weight protein is pulverized to a particle size similar to that of a peptide, and the protein The specific surface area of the particles is greatly expanded, dramatically increasing the enzyme decomposition efficiency and significantly shortening the enzyme decomposition time.

In addition, according to the present invention, it is possible to quickly and stably raise and maintain the temperature of the protein solution in the protein digestion tank using a temperature maintenance means, and to efficiently measure temperature, pH, ultrasound management, and protein concentration and amino acid concentration in real time. It has the effect of promoting enzyme reactions and increasing the production rate of peptides and amino acids.

Ultimately, according to the peptide and amino acid production device of the present invention, the production time of peptides and amino acids is significantly shortened compared to the existing one, and the production rate and production cost of high-purity amino acids are greatly improved, so that high-purity amino acids can be used in various fields such as food, cosmetics, and pharmaceuticals. There is a chain effect that can be easily applied to.

Figure 1 is an exemplary diagram of a protein digestion tank according to the peptide and amino acid production apparatus of the present invention.
2 to 3 are internal configuration diagrams of a protein digestion tank according to the peptide and amino acid production apparatus of the present invention.
Figure 4 is a partial configuration diagram of a protein digestion tank according to the peptide and amino acid production apparatus of the present invention.
Figure 5 is a structural diagram of an ultrasonic generator according to the prior art.
Figure 6 is a structural diagram of an ultrasonic generator according to the peptide and amino acid production apparatus of the present invention.
Figure 7 is an exemplary diagram showing two protein digestion tanks connected according to the peptide and amino acid production apparatus of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the attached drawings. Prior to this, the terms or words used in this specification and claims should not be construed as limited to their usual or dictionary meanings, and the inventor should appropriately use the concept of terms to explain his or her invention in the best way. It must be interpreted as meaning and concept consistent with the technical idea of the present invention based on the principle of definability. Therefore, the embodiments described in this specification and the configurations shown in the drawings are only one of the most preferred embodiments of the present invention and do not represent the entire technical idea of the present invention, so at the time of filing the present application, various alternatives are available. It should be understood that equivalents and variations may exist.

According to the conventional enzyme decomposition method, due to the lack of experiments on numerous enzymes that decompose proteins, there was no distinction in the use of enzyme types such as endo-type enzymes and exo-type enzymes, and mainly one type of enzyme was used. In most cases, it was only used. In addition, even if the protein is decomposed by this method, there is a disadvantage that a significant amount of the polymer protein is not converted to amino acids.

In addition, conventionally, in the decomposition of a protein solution, it takes more than 2 hours just to heat the temperature to approximately 60°C, and 60°C must be maintained continuously for a long time, but the temperature between the center and the periphery of the protein decomposition tank containing the soluble solution must be maintained. Temperature differences often occur, which can inhibit enzyme reactions and reduce productivity.

Therefore, the present invention was conceived to overcome the shortcomings of the prior art and to provide a faster and more efficient protein decomposition system, including the type of enzyme, temperature maintenance, and PH maintenance.

1 to 3 are illustrations and internal configuration diagrams of protein digestion tanks according to the peptide and amino acid production apparatus of the present invention.

The peptide and amino acid production apparatus of the present invention includes a protein decomposition tank (100) that stores a protein solution and has an enzyme injection device (20) capable of introducing an enzyme that decomposes the protein solution into peptides or amino acids, and a protein decomposition unit. A side stirrer 50 is installed on the side of the tank and rotates to agitate the protein solution, and is installed at the top of the protein digestion tank 100 and rotates at a predetermined angle with the side stirrer to rotate and agitate the protein solution. An upper stirrer (21), a temperature maintaining means for maintaining the temperature of the protein solution in the protein digestion tank (100) constant, an ultrasonic generator (30) for applying ultrasonic waves to the protein solution, and the temperature maintaining means, a first stirrer (50) ), and a control unit 19 that controls the operation of the second stirrer 21 and the ultrasonic generator 30.

The protein digestion tank 100 is a protein solution storage tank (not shown), which stores a protein solution formed by dry grinding silkworm or insect protein powder and mixing it with water, and a protein solution storage tank (not shown) and a storage tank connection 91. can be supplied.

More specifically, the protein solution is made by dry grinding protein powder mixed with silkworms or insects, mixing the dry grinded protein powder and water at a certain ratio (e.g. 2:8), and then grinding it in a wet grinder (92). It can be introduced into the protein decomposition tank (100) while being wet-grinded.

That is, the protein solution supplied through the storage tank connection 91 can be wet-ground through a wet grinder 92, which transfers the wet-ground protein particles to the ultrasonic generator 30 to further ultrasonic grind the protein. This is to make it easier.

In the present invention, any one or more of the silkworms, silkworm cocoons or their powders, dried silkworms or their powders, and red silkworms or their powders may be applied.

In addition, the above insects may be double-star crickets, grasshoppers, silkworm pupae, white snails, mealworms, white-spotted radish caterpillars (slugs), brown mealworm larvae, rhinoceros beetle larvae, drone pupae, grasshoppers, or powders thereof. It is not necessarily limited to this, and any insect species that contains protein components may be included.

Referring to FIG. 1, the present invention includes sensors (11, 12, 14, 15, 16, 17) that measure water level, temperature, pH, protein concentration, amino acid concentration, and ultrasound in the protein digestion tank (100). ), but its operation is controlled by the control unit 19.

Various sensors (11, 12, 14, 15, 16, 17) that can determine the state of the protein solution are installed in the protein digestion tank (100) that can contain the protein solution, and the control unit (19) and the electrical It is connected to The control unit 19 uses a temperature maintaining means and an upper stirrer ( 21), controls the operation of the side stirrer 50 and the ultrasonic generator 30.

The water level measurement sensor 11 is a sensor that measures and displays the water level of the protein solution introduced into the protein digestion tank 100. It accurately measures and displays the water level of the protein solution and manages it to standardize the water level of the protein solution. It performs the function of providing preliminary information on the temperature, pH, enzyme reaction, etc. of the protein solution.

The temperature sensor 12 separately checks the temperature of each area, such as the core, upper layer, lower layer, and edge, of the protein solution in the protein digestion tank 100 to help maintain the optimal temperature of the solution in the protein digestion tank.

The PH sensor 14 performs the function of checking and displaying the PH of the protein solution in the protein digestion tank 100, checks changes in PH, and allows the user to respond to the PH changes to optimize the enzyme reaction. Assist.

In the present invention, protein concentration is an important factor in determining the amount of enzyme input, and as the enzyme reaction progresses, protein concentration decreases. The protein concentration sensor 15 measures and displays increases and decreases in protein concentration, providing accurate information about protein degradation. Assist with information management and time management.

The ultrasonic sensor 16 displays information such as the frequency range of ultrasonic irradiation to the protein solution by the ultrasonic generator 30, the start, operation, and end of ultrasonic treatment, and assists in pulverizing protein particles into fine particles. do.

The amino acid concentration sensor 15 assists in accurately recognizing the amino acid production rate by indicating changes in amino acid concentration as proteins are decomposed and displaying the change in amino acid concentration.

The control unit 19 is equipped with a separate controller panel so that various sensors mounted on the protein digestion tank can be easily controlled in the field, and the protein digestion tank 100 has a separate ON/OFF switch (18). ), the operation of the protein digestion tank can be started and stopped.

The sensors (11, 12, 14, 15, 16, 17) and the control unit (19) that measure the water level, temperature, pH, protein concentration, amino acid concentration, and ultrasonic waves are connected and operated by a remote central computer, It can be controlled.

Among the factors that affect the reaction rate and decomposition rate in protein hydrolysis, it is important to properly stir the protein solution. In other words, since the amount of enzyme added is very small compared to the entire protein solution, a small amount of enzyme must quickly spread throughout the solution to decompose the protein particles. However, most of the existing stirring propellers are unidirectional, so the enzyme spreads throughout the solution. It took a considerable amount of time to do this, but by using the top/side stirrer of the present invention at the same time, a small amount of enzyme can be quickly and evenly spread throughout the solution, thereby improving the enzyme decomposition reaction.

The side stirrer 50 is installed on the side of the protein digestion tank and performs the function of rotating and agitating the protein solution.

The side stirrer 50 is installed horizontally in the protein digestion tank, and the stirring propeller 51 rotates and agitates the protein solution in the vertical direction. At least two side stirrers 50 may be installed on the side of the protein digestion tank.

The upper stirrer 21 is installed at the top of the protein digestion tank 100 and functions to rotate and agitate the protein solution by rotating at a predetermined angle with the side stirrer.

Referring to FIG. 3, the upper stirrer 21 preferably rotates and stirs the protein solution at an angle of 45 degrees with the side stirrer 50. In this way, if the upper stirrer 21 is rotated and stirred at an angle Θ of 45 degrees with the side stirrer 50 and the rotation axis of the upper stirrer 21 draws a circle when viewed from the top, it is easier to introduce the enzyme. It will be able to spread evenly into the solution.

It is preferable that the stirring speed of the stirrers is in the range of 60 to 100 rpm, and the upper stirrer 21 and the side stirrer 50 are staggered at a predetermined angle to simultaneously stir the protein solution, thereby preventing the concentration of the protein solution liquid. This phenomenon does not occur, and all of the upper and lower layers and the left and right sides of the protein decomposition tank 100 can be completely mixed and stirred.

However, depending on the needs of the invention, the upper stirrer 21 may rotate and stir the protein solution at an angle of 90 degrees with the side stirrer 50.

An enzyme injection device 20 may be formed on the upper part of the upper stirrer 21, and a plurality of stirring propellers may be formed on the lower part of the upper stirrer 21 so that a plurality of enzyme outlets can be formed.

The enzyme introduction device 20 is rotary and functions to increase enzyme reaction efficiency and shorten reaction time by injecting enzyme and rotating the upper stirrer 21 to spread the enzyme evenly on the upper surface of the protein solution.

In this way, the protein digestion tank 100 continuously mixes the protein solution in the longitudinal, horizontal and diagonal directions by two types of stirrers installed at a predetermined angle, and uses ultrasonic waves generated by the ultrasonic generator 30 to dissolve the protein. Since the particles are pulverized into smaller and smaller particles, the enzyme reaction proceeds quickly and evenly on all protein particles, thereby maximizing the protein decomposition effect.

The cover 90 of the protein digestion tank 100 may be a hydraulic cover, and must be made of stainless steel with a thickness in the range of 6-10 mm. Therefore, due to its weight, it must be opened and closed hydraulically.

Meanwhile, as the protein solution in the protein decomposition tank 100 is heated and circulated, a phenomenon may occur in which organic matter such as protein sticks to the inner circumference of the tank and does not come off easily. This phenomenon is also an inhibitor to enzyme reactions and causes decay over time. Therefore, the inner surface of the tank is coated with materials such as fluorine resin or carbon resin to prevent organic substances from sticking to it. The protein digestion tank 100 may be formed using stainless steel.

Figure 4 is a partial configuration diagram of a protein digestion tank according to the peptide and amino acid production apparatus of the present invention.

Temperature is a very important factor in enzyme reactions for proteins. If a typical temperature maintaining device is adopted, the temperature at the center and the edge of the protein digestion tank 100 may differ, and as the size or capacity of the reaction tank increases, the temperature increases. It is common for the temperature difference between the center and the edge to increase.

Therefore, in the present invention, in order to increase the efficiency of the enzyme reaction, a temperature maintenance means is installed and a hot water boiler system as well as a circulating instantaneous heating system are operated in parallel, so that the temperature is maintained at 55°C to 60°C within a short time regardless of the center and edge of the protein decomposition tank. By maintaining the temperature rise, the enzyme reaction rate and amino acid production rate can be increased.

In the present invention, the temperature maintenance means supplies hot water to the hot water path 80 formed along the outer peripheral surface of the protein decomposition tank through the boiler 93, and heats the protein to the instantaneous heater 70 mounted on the side of the protein decomposition tank. It functions to raise and maintain the protein solution at 55℃~60℃ by supplying the solution in a circulatory manner.

To this end, the protein solution is continuously supplied to the instantaneous heater 70 through the second circulation motor 62 located at the bottom of the side of the protein digestion tank, and the protein solution whose temperature has risen is fed back into the protein digestion tank. . The second circulation motor 62 performs the function of circulating and supplying the protein solution to the instantaneous heater 70 through a pipe provided on the lower side of the protein decomposition tank.

In the present invention, the protein digestion tank 100 maintains the stored protein solution at a temperature of 55-60°C and a pH of 6.0-8.0 while stirring the stored protein solution at a speed of 60-100 rpm by the first and second stirrers. .

That is, in the present invention, during the enzyme reaction process, the temperature in the protein digestion tank is continuously maintained at 55°C to 60°C by a temperature maintenance means, and the upper stirrer (21) and the side stirrer (50) mounted on the protein digestion tank (100) ) The stirring speed of the propeller is set to 60-100 rpm, and the stirring directions of the upper stirrer (21) and the side stirrer (50) are alternated with each other. This action causes the temperature of the protein solution in the protein digestion tank (100) to change. Deviations will be further reduced. Therefore, the protein decomposition effect of the enzyme ultimately introduced into the protein decomposition tank 100 can be maximized.

In this way, the conventional temperature maintenance method heats the protein decomposition tank 100 solely using a hot water boiler system, and it takes several hours or more depending on the capacity of the reaction tank just to heat it to 60°C, and the heated solution Also, a temperature difference occurred between the core and the edge of the reaction tank, which was a factor in inhibiting the productivity of the enzyme reaction.

In the present invention, an instantaneous heating method is adopted to raise the protein solution to 60°C within 5 to 10 minutes, which greatly contributes to improving productivity by significantly shortening the solution heating time, and the protein solution with the increased temperature is returned to the protein decomposition tank ( 100), the temperature difference between the protein solutions in the protein digestion tank is prevented by being circulated, thereby improving the enzyme reaction by eliminating factors that inhibit the enzyme reaction due to temperature changes.

Furthermore, the present invention can contribute to improving productivity by significantly shortening the conventional heating time and reducing energy costs by applying not only the hot water boiler system but also the instantaneous heater 70.

The instantaneous heater 70 is installed between the storage tank (not shown) and the protein digestion tank 100, and between the protein digestion tank 100 and the second protein digestion tank 200 according to the needs of the invention, so that the protein solution flows into the tank. Alternatively, the temperature can be maintained even if it is moved.

Figure 5 is a structural diagram of an ultrasonic generator according to the prior art.

Conventionally, an ultrasonic vibrator 32 was installed within the protein digestion tank 100 and ultrasonic waves were applied to the protein solution through an ultrasonic controller 31 from outside the tank.

In other words, such a bath type ultrasonic generator operates by being sealed and installed inside a stirred tank, but the efficiency of heat control during ultrasonic reaction is low, the cavitation effect is minimal, and the protein solution in the sealed tank is low. The method of irradiating while stirring had the disadvantages of being difficult to install, having low durability, and making it difficult to implement various frequencies.

On the other hand, the circulating ultrasonic method adopted by the present invention is installed on the outside of the protein digestion tank 100 and irradiates ultrasonic waves while circulating the solution in the tank, and is different from the bath type ultrasonic method installed inside the tank. In contrast, temperature control is easy, the protein solution is irradiated with ultrasonic waves at 360 degrees, so grinding efficiency is high, and the strong cavitation effect is advantageous for fine grinding, so it has the advantage of maximizing the grinding efficiency of protein particles.

Figure 6 is a structural diagram of an ultrasonic generator according to the peptide and amino acid production apparatus of the present invention.

In the present invention, the ultrasonic generator 30 includes an ultrasonic reactor 41 into which a protein solution is introduced and discharges the introduced protein solution into a protein decomposition tank, and a cooling system surrounding the ultrasonic reactor and allowing cooling water to flow in and out. It is desirable to include a device 40, an ultrasonic vibrator 32 surrounding a portion of the side of the cooling device, and an ultrasonic generator cover 42 covering the ultrasonic reactor, the cooling device, and the ultrasonic vibrator.

The ultrasonic generator 30 of the present invention grinds silkworm or insect polymer protein particles ranging from 100,000 Da to 300,000 Da into ultra-fine particles to the level of low-molecular peptides, greatly increasing the specific surface area of the protein particles, thereby dramatically increasing the enzyme reaction speed and amino acid production rate. It contributes to increasing the , and furthermore, it is possible to break away from the unproductive method of separately decomposing high-molecular-weight and low-molecular-weight proteins.

Meanwhile, in the existing bath-type ultrasonic method, there is a high possibility that ultrasonic waves are not radiated evenly depending on the size and capacity of the tank, and it is difficult to pulverize all protein particles to the level of low-molecular peptides during the first ultrasonic pulverization. Therefore, in the present invention, since the circulating ultrasonic irradiation method is adopted to continuously circulate the protein solution while ultrasonic pulverization is performed, it is easy to pulverize almost all protein particles to the level of low-molecular-weight peptides.

For this purpose, in the present invention, the protein solution stored in the protein decomposition tank 100 is introduced into the ultrasonic reactor 41 in a circulatory manner by the first circulation motor 61.

In addition, since high heat is generated during ultrasonic pulverization of protein particles and may affect the enzyme reaction, the present invention is equipped with a cooling device 40 to offset heat generation and manage the temperature at an appropriate temperature so as not to affect the enzyme reaction. Take care not to

For this purpose, a cooling device 40 through which coolant flows in and out is formed between the ultrasonic reactor 41 and the ultrasonic vibrator 32. The coolant having a coolant inlet and a coolant outlet is a protein decomposition tank 100. It is preferable to be formed outside the protein decomposition tank 100 so as not to affect the temperature.

Ultrasonic pulverization using the ultrasonic generator 30 irradiates ultrasonic waves with a frequency ranging from 20 KH to 40 KH for 20 to 60 minutes depending on the capacity of the circulating protein solution, and the polymer protein particles are ultrafine to the peptide level.

In this way, the circulating ultrasonic method of the present invention is installed outside the protein decomposition tank 100 and circulates the protein solution along the ultrasonic reactor 41 while irradiating ultrasonic waves to pulverize it. It is a method of pulverizing the protein solution by irradiating it with ultrasonic waves. The structure itself is completely different from the bath type ultrasonic method, and the operating mechanism is also completely different.

That is, the cyclic ultrasonic irradiation method of the present invention is i) easy to control the heat generated during the ultrasonic reaction of protein particles, thereby preventing the influence of heat during enzyme decomposition, and ii) ultrasonic waves around the ultrasonic reactor 41. Since the oscillator 32 is installed to irradiate ultrasonic waves at 360 degrees, it can bring about the effect of powerful cavitation fine grinding. iii) Even if an ultrasonic oscillator made of the same titanium material is applied, the durability life of the existing bath type is less than 1,000 hours. In contrast, the cyclic ultrasonic irradiation method has the advantage of having a durability of more than 10,000 hours.

In the ultrasonic generator 30, when an ultrasonic vibrator 32 installed on the outside of the protein decomposition tank 100 generates ultrasonic waves in the protein solution, various bacteria, including pathogenic bacteria, are destroyed by the energy of the ultrasonic waves generated at this time, thereby achieving a sterilization effect. It can be obtained and can act as another major factor that increases the efficiency of the enzyme reaction by preventing degradation of the enzyme reaction by pathogenic bacteria during the enzyme reaction.

The previous sterilization method involved putting a protein solution into a reaction tank, heating it to 100°C, performing a sterilization process for more than an hour, and then lowering the temperature again. This was a wasteful method that required a lot of energy, process steps, and process time. However, in the sterilization process, irradiating the protein solution with ultrasound can prevent waste in the above process and greatly increase productivity.

Meanwhile, in the present invention, the ultrasonic generator 30 can radiate ultrasonic waves with a frequency of 20 to 40 kHz to inject enzymes into the protein digestion tank 100 and stop the activity of the introduced enzymes after a predetermined period of time. .

In the present invention, the enzyme reaction can be carried out by simultaneously adding the endo type enzyme Alcarase and the Exo type enzyme Flavorzyme. For example, after adding two enzymes In order to stop the activity of the enzymes added after 4 hours, the activity of the enzymes can be stopped by irradiating ultrasound with a frequency of 20 to 40 kHz for 15 to 25 minutes through the ultrasonic generator 30.

Figure 7 is an exemplary diagram showing two protein digestion tanks connected according to the peptide and amino acid production apparatus of the present invention.

FIG. 7 shows that a second protein digestion tank 200 of the same structure is installed redundantly after the protein digestion tank 100, so that the enzyme reaction process can be divided into first and second stages. That is, at the rear of the protein digestion tank 100, at least one second protein digestion tank 150 having the same configuration as the protein digestion tank 100 is installed to perform a plurality of enzyme reaction processes. .

In other words, the protein digestion tank 100 can sequentially decompose proteins by connecting at least two protein digestion tanks, and at this time, an instantaneous heater (70) is installed between the two or more protein digestion tanks to maintain the temperature of the protein solution. ) can be installed.

In the present invention, two types of enzymes are added simultaneously. The Endo type enzyme is used to decompose proteins, and the Exo type enzyme is used to produce amino acids. However, after a certain period of time, the vitality of the two enzymes decreases. A secondary process can be performed to increase the vitality of the enzymes. In order to reduce the overall process time, the protein solution that has completed the primary process can be processed into a second process. It is possible to perform a second process by transferring it to the protein digestion tank 200.

Therefore, according to one embodiment of the present invention, the first enzyme reaction process is to heat the protein solution and add endo-type enzyme and exo-type enzyme to the protein solution. Peptides and amino acids can be produced through a second enzyme reaction process in which endo-type enzymes and exo-type enzymes are added again.

As described above, the present invention applies a three-stage grinding technology of 'dry grinding, wet grinding, and ultrasonic grinding' to protein powder to ultrafine the polymer protein particles and greatly expands the specific surface area of the protein particles to improve enzymatic decomposition time and efficiency. can be dramatically increased. However, just because the protein is decomposed by the grinding process does not mean that all of it is amino acidized, and both endo-type enzymes, which decompose proteins well in a short time, and exo-type enzymes, which increase the amino acid production rate, are appropriately added. This causes an enzyme reaction to occur.

In the present invention, the first enzyme reaction process involves adding 2 to 3% by weight of Endo type enzyme and 0.2 to 0.5% by weight of Exo type enzyme to the protein digestion tank (100) based on the weight of the pulverized protein solution. Once added, the enzyme is reacted with the protein solution within 3 hours while adjusting the pH between 6 and 8.

In the conventional method, the enzyme reaction was usually carried out at pH 7. Since the pH at which protein decomposition is good and the pH at which amino acid production is good are different, the pH concentration is adjusted according to the time by considering the protein decomposition rate and amino acid production rate during the enzyme reaction. It must be adjusted accordingly to achieve the best productivity and efficiency.

For example, in the first enzyme reaction step of the present invention, pH is maintained at 8 for 1 hour after enzyme addition to accelerate protein decomposition and amino acid production, and pH is maintained at 6.0 to 6.5 for 1 to 2 hours, and the peptide solution is commercialized. PH can be maintained at 7 for up to 2 to 3 hours as a rest period. A low-purity peptide solution can be extracted from the first enzyme reaction process, and it is also possible to commercialize it separately.

The protein solution that has completed the first enzyme reaction process can be moved from the protein digestion tank 100 to the second protein digestion tank 200 to immediately proceed with the second enzyme reaction process.

Experimentally, the Endo type enzyme is used to decompose proteins and the optimal pH is 8.0, and the Exo type enzyme is used to produce amino acids and the optimal pH is 6.0 to 6.5. .

However, if more than 2 hours have passed since the above enzyme reaction, the vitality of each enzyme decreases and the enzyme reaction decreases accordingly. New endo type enzyme Alcarase 1%-2% and exo type enzyme Alcarase 1%-2% (Exo type) A second enzyme reaction process that adds vitality to the enzyme can be performed by adding 0.1%-0.2% of the enzyme flavourzyme.

In the second enzyme reaction, the pH is maintained at 7 for up to 1 hour after enzyme addition. In the present invention, the second enzyme reaction is not for accelerating protein decomposition or amino acid production, but for final decomposition of the remaining amount of undigested protein. For this purpose, the pH is set to 7 and the enzyme reaction is carried out.

Meanwhile, the second enzyme reaction process involves irradiating ultrasound with a frequency of 20 to 40 kHz for 15 to 25 minutes through the ultrasonic generator 30 to stop the activity of the introduced enzyme 1 hour after the enzyme is added. desirable.

This is because the enzyme activity must be stopped once the secondary enzyme reaction process is completed, and if the enzyme activity is not stopped, it may remain in the product and affect it. Energy costs and time can be greatly reduced by using ultrasound to stop the enzyme activity. This can improve productivity and reduce costs. After the second enzyme reaction process as above is completed, a mixture of low-molecular-weight peptides and amino acids is obtained, which can be packaged and commercialized.

Meanwhile, the undecomposed proteins and impurities contained in the amino acid peptide mixed solution are coagulated using chitosan or activated carbon and filtered, and the filtered amino acid peptide mixed solution is purified through a filter press (not shown) to obtain a high purity amino acid solution. You can. When the peptide/amino acid solution from which undigested proteins and impurities have been removed is passed through a filter press, a high-purity amino acid solution and an organic cake are formed. The organic cake can be processed into a high-purity amino acid powder through a microwave drying process.

In this way, the present invention provides ultra-fine particle size of protein particles for highly efficient amino acid production, consistent temperature management of the protein solution to create the best environment for enzymatic decomposition, pH management for efficient protein decomposition and amino acid production, and input enzymes. High purity amino acids can be produced by optimizing the type, reaction time, etc.

Although the present invention has been described above in relation to specific embodiments of the present invention, this is only an example and the present invention is not limited thereto. A person of ordinary skill in the technical field to which the present invention pertains may change or modify the described embodiments without departing from the scope of the present invention, and within the scope of equivalency of the technical idea of the present invention and the scope of the patent claims described below. Various modifications and variations are possible.

11: water level measurement sensor 12: temperature sensor
14: PH sensor 15: Protein concentration sensor
16: ultrasonic sensor 17: amino acid concentration sensor
18: ON/OFF switch 19: Control unit
20: Enzyme injection device 21: Upper stirrer
30: Ultrasonic generator 31: Ultrasonic controller
32: ultrasonic transducer 40: cooling device
41 Ultrasonic reactor 42: Ultrasonic generator cover
50: side agitator 51: stirring propeller
61: first circulation motor 62: second circulation motor
70: instantaneous heater 80: hot water furnace
90: Cover 91: Storage tank connection
92: wet grinder 93: boiler
100: protein digestion tank 200: second protein digestion tank

Claims (14)

A protein decomposition tank (100) that stores a protein solution and is equipped with an enzyme injection device (20) capable of introducing an enzyme that decomposes the protein solution into peptides or amino acids;
A side stirrer (50) installed on the side of the protein digestion tank to rotate and agitate the protein solution;
An upper stirrer (21) installed at the top of the protein digestion tank (100), which rotates the protein solution by rotating at a predetermined angle with the side stirrer (21);
Temperature maintenance means for maintaining the temperature of the protein solution in the protein decomposition tank (100) at a constant level;
An ultrasonic generator (30) that applies ultrasonic waves to the protein solution; and
A control unit 19 that controls the operation of the temperature maintenance means, the first stirrer 50, the second stirrer 21, and the ultrasonic generator 30;
A peptide and amino acid manufacturing device comprising a.
The method of claim 1, wherein the ultrasonic generator 30 is:
An ultrasonic reactor (41) into which a protein solution is input and which discharges the input protein solution into a protein decomposition tank;
A cooling device 40 surrounding the ultrasonic reactor and into which cooling water flows in and out;
An ultrasonic vibrator (32) surrounding a portion of the side of the cooling device; and
An ultrasonic generator cover 42 covering the ultrasonic reactor, cooling device, and ultrasonic oscillator;
A peptide and amino acid production device comprising:
According to paragraph 2,
The protein solution introduced into the ultrasonic reactor 41 is a protein solution that has passed through a wet grinder 92 or a protein solution stored in the protein decomposition tank 100 is introduced by the first circulation motor 61. Characterized by a peptide and amino acid production device.
The method of claim 1, wherein the ultrasonic generator 30 is:
A peptide and amino acid production device, characterized in that irradiating ultrasonic waves with a frequency in the range of 20 KH to 40 KH for a certain period of time to the protein solution.
The method of claim 1, wherein the ultrasonic generator 30 is:
A peptide and amino acid production device characterized by adding an enzyme to the protein digestion tank and irradiating ultrasound with a frequency of 20 to 40 kHz to stop the activity of the added enzyme after a predetermined time.
According to paragraph 1,
The protein digestion tank 100 further includes sensors 11, 12, 14, 15, 16, and 17 that measure water level, temperature, pH, protein concentration, amino acid concentration, and ultrasound, and the control unit ( 19) A peptide and amino acid production device, characterized in that the operation is controlled by.
The method of claim 1, wherein the temperature maintaining means is:
Hot water is supplied to the hot water passage (80) formed along the outer peripheral surface of the protein decomposition tank through the boiler (93),
A peptide and amino acid manufacturing device characterized in that the protein solution is supplied in a circulatory manner to an instantaneous heater (70) mounted on the side of the protein decomposition tank to raise and maintain the protein solution at 55°C to 60°C.
In clause 7,
The protein solution is continuously supplied to the instantaneous heater (70) through the second circulation motor (62) located at the bottom of the side of the protein digestion tank, and the protein solution with an increased temperature is fed back into the protein digestion tank. Peptide and amino acid manufacturing equipment.
The method of claim 1, wherein the protein digestion tank (100),
A peptide and amino acid production device characterized in that the stored protein solution is stirred at a speed of 60 to 100 rpm by a first stirrer and a second stirrer, while maintaining a temperature of 55 to 60 ° C and a pH of 6.0 to 8.0.
According to paragraph 1,
The side stirrer (50) is installed horizontally in the protein digestion tank, and the stirring propeller (51) rotates and agitates the protein solution in the vertical direction.
According to paragraph 1,
The upper stirrer (21) is a peptide and amino acid production device, characterized in that the protein solution is rotated and stirred at an angle of 45 degrees with the side stirrer (50).
The method of claim 1, wherein the protein digestion tank (100),
A peptide and amino acid production device characterized in that at least two protein digestion tanks are interconnected to sequentially decompose proteins.
The method of claim 1, wherein the enzyme is:
A peptide and amino acid manufacturing device characterized by containing Alcarase as an endo type enzyme and Flavozyme as an exo type enzyme.
According to paragraph 1,
The protein solution is a peptide and amino acid production device characterized in that it is formed by dry grinding silkworm protein powder, insect protein powder, or protein powder mixed with silkworm and insect, and then adding water and wet grinding.