KR20230167595A - Collagen-contaning liquid composition and method for preparing the same - Google Patents

Abstract

The present invention relates to a collagen-containing liquid composition and a method for producing the same. More specifically, the collagen-containing liquid composition includes black carrot extract and/or ingredients that can prevent color changes in the composition itself due to external environments such as light. Alternatively, since it contains prune extract, the collagen-containing liquid composition can be stored stably with less color change even when applied to a recyclable transparent PET container.

Description

Collagen-containing liquid composition and manufacturing method thereof {COLLAGEN-CONTANING LIQUID COMPOSITION AND METHOD FOR PREPARING THE SAME}

The present invention relates to a liquid composition containing collagen and a method for producing the same.

Because collagen is involved in the amount of skin moisture, it is known that consuming collagen can prevent skin aging, joint weakness, and blood vessel damage. Taking advantage of the efficacy of collagen, various functional products using collagen as a raw material are being developed.

In general, collagen liquid products are mainly commercialized and sold in plastic containers.

The plastic container currently in use is made of PET-PEN (polyethylene terephthalate-polyethylene naphthalate), which is capable of blocking light and can stably store contents containing liquid formulations. Therefore, it was mainly used as a container for liquid products, but there is a problem of non-recycling. there was.

When changing the PET-PEN material container to a recyclable transparent PET container, recycling of the plastic container becomes possible and it has been confirmed that the amount of plastic used is reduced by about 65% compared to the existing plastic container. There was an attempt to change to PET containers.

However, when applying collagen liquid products to transparent PET containers, a problem occurred in that the color of the contents inside the PET container changed significantly due to light.

Accordingly, there is a need for the development of collagen liquid products that have excellent stability even when applied to recyclable transparent PET containers and can prevent color change due to light.

Japanese Patent No. 6296792

In order to solve the above problem, the present inventors found that when black carrot extract and/or prune extract is applied to a collagen-containing liquid composition, discoloration due to external environments such as sunlight is minimized and the stability of the collagen-containing liquid composition is strengthened. Confirmed.

Therefore, the purpose of the present invention is to provide a collagen-containing liquid composition with enhanced stability and a method for producing the same.

In order to achieve the above object, the present invention provides (i) collagen; and (ii) at least one selected from black carrot extract and prune extract.

The collagen-containing liquid composition according to the present invention, due to its composition including black carrot extract and/or prune extract, minimizes discoloration of the liquid composition due to external environments such as sunlight, which can have the effect of enhancing stability. there is.

Figure 1 shows the color difference of the liquid compositions prepared in Examples and Comparative Examples by storage period.

Hereinafter, the present invention will be described in more detail.

Liquid composition containing collagen

The present invention relates to (i) collagen; and (ii) at least one selected from black carrot extract and prune extract. When the collagen-containing liquid composition includes both black carrot extract and prune extract, the effect of improving the discoloration of the liquid composition can be significantly improved.

In the present invention, the collagen provides strength and tension to the skin and plays a role in protecting the skin, and is related to skin beauty and anti-aging. Animal collagen, fish collagen, etc. used in oral collagen products are widely used. can be used

For example, the collagen may be fish collagen, and the absorption rate may be better when fish collagen is used.

In addition, the collagen may be a collagen peptide, and the collagen peptide may have an average molecular weight of 1000 Da to 2000 Da, specifically 1000 Da or more, 1200 Da or more, or 1400 Da or more, and 1600 Da or less. , may be 1800 Da or less, or 2000 Da or less, preferably 1500 Da.

In addition, the collagen contains glycine-proline-hydroxyproline (GPH), and the GPH may be included in an amount of 2.5% by weight or more based on the total weight of the collagen. Specifically, the content of the GPH It may be more than 2.5% by weight, more than 2.8% by weight, more than 3.0% by weight, more than 3.2% by weight, or more than 3.4% by weight, and when included in the above content range, it may have the advantage of excellent absorption in the body when ingested orally. The upper limit of the GPH content is not particularly limited, but may be 4.2% by weight or less.

The collagen peptide is a complex containing three amino acids, “glycine-x-y,” where x and y can be any amino acid, and x and y can be the same or different amino acids. That is, alanine (Ala), valine (Val), leucine (Leu), isoleucine (Ile), proline (Pro), and hydroxyproline (Hyp) that exist naturally. ), phenylalanine (Phe), tryptophan (Trp), methionine (Met), serine (Ser), threonine (Thr), cysteine (Cys), glutamine (Gln) , glycine (Gly), asparagine (Asn), tyrosine (Tyr), lysine (Lys), arginine (Arg), histidine (His), aspartic acid, Amino acids such as Asp) and glutamic acid (Glu) can all be used, but preferably Gly-Pro-Hyp, Gly-Pro-Ala, Gly-Ala-Hyp, Gly-Leu-Hyp, Gly-Glu- It can be used in the composition of Lys, Gly-Pro-Lys, Gly-Glu-Hyp, Gly-Phe-Hyp, Gly-Ser-Hyp, Gly-Gln-Hyp and Gly-Glu-Arg, Gly-Pro-Arg. Preferably, glycine-proline-hydroxyproline (glycine-proline-hydroxyproline, GPH) may be linked.

In addition, the collagen may be included in an amount of 0.1% to 10% by weight based on the total weight of the composition, and specifically, the collagen content is 0.1% by weight or more, 1% by weight, 2% by weight or more, or 3% by weight. It may be more than 6% by weight, 8% by weight or less, or 10% by weight or less. If the collagen content is less than 0.1% by weight, the inherent effects of collagen, such as improving skin elasticity, may not be significant, and if the collagen content is more than 10% by weight, taste preference may be reduced.

In the present invention, the black carrot extract can be obtained by extracting or squeezing one or more of the leaves, stems, flowers, and fruits of black carrots.

The black carrot contains a large amount of anthocyanin. The anthocyanin is a pigment contained in flowers, fruits, etc., and when the black carrot extract is applied to a collagen-containing liquid composition, the anthocyanin plays a light-shielding role and prevents discoloration of the collagen-containing liquid composition, thereby improving stability.

In addition, the black carrot extract may be in the form of a black carrot concentrate, and the black carrot concentrate may have a concentration of 60 Brix to 75 Brix. Specifically, the sugar content of the black carrot concentrate is 60 Brix or more, 62 Brix or more, or 64 Brix. It may be Brix or higher, or it may be 71 Brix or lower, 73 Brix or lower, or 75 Brix or lower. If the sugar content of the black carrot concentrate is less than 60 Brix, the discoloration prevention effect of the collagen-containing liquid composition may be minimal, and if it is more than 75 Brix, the formulation stability and emotional quality of the raw material may be reduced.

Additionally, the black carrot extract may be included in an amount of 0.1% to 5% by weight based on the total weight of the composition. Specifically, the content of the black carrot extract may be 0.1% by weight or more, 0.3% by weight or more, 0.5% by weight or more, or 1.0% by weight or more, and 1.8% by weight or less, 2.0% by weight or less, 3.0% by weight or less, and 4.0% by weight. It may be less than or equal to 5.0% by weight. If the content of the black carrot extract is less than 0.1% by weight, the shading effect against external environments such as sunlight may not be significant, and if it is more than 5.0% by weight, the formulation stability and emotional quality of the product may be reduced.

In the present invention, the prune extract can be obtained by extracting or squeezing the fruit of the prune, and preferably, it can be obtained by extracting or squeezing the pulp surrounding the seed from the fruit of the prune.

The prunes contain a large amount of anthocyanin. The anthocyanin is a pigment contained in flowers, fruits, etc., and when the prune extract is applied to a collagen-containing liquid composition, the anthocyanin plays a light-shielding role and prevents discoloration of the collagen-containing liquid composition, thereby improving stability.

In addition, the prune extract may be in the form of a prune concentrate, and the prune concentrate may have a concentration of 65 Brix to 75 Brix. Specifically, the sugar content of the prune concentrate may be 65 Brix or more, 66 Brix or more, or 67 Brix or more. , or may be 73 Brix or less, 74 Brix or less, or 75 Brix or less. If the sugar content of the prune concentrate is less than 60 Brix, the discoloration prevention effect of the collagen-containing liquid composition may be minimal, and if it is more than 75 Brix, the formulation stability and emotional quality of the raw material may be reduced.

Additionally, the prune extract may be included in an amount of 0.1% to 5% by weight based on the total weight of the composition. Specifically, the content of the prune extract may be 0.1 weight% or more, 0.3 weight% or more, 0.5 weight% or more, or 1.0 weight% or more, and 1.8 weight% or less, 2.0 weight% or less, 3.0 weight% or less, and 4.0 weight% or less. Or it may be 5.0% by weight or less. If the content of the prune extract is less than 0.1% by weight, the shading effect against external environments such as sunlight may not be significant, and if it is more than 5.0% by weight, the formulation stability and emotional quality of the product may be reduced.

In the present invention, the color change of the collagen-containing liquid composition is calculated as the color difference (ΔE ^* _ab ) defined by the following equation 1, and the color difference may be 12 or less, specifically, 12 or less, 11 or less, 10 or less, It can be 8 or less or 7 or less:

<Equation 1>

In Equation 1 above, L ^* ₁ , a ^* ₁ , b ^* ₁ and L ^* ₂ , a ^* ₂ , b ^* ₂ refer to colorimeter values measured at different points in time at certain time intervals. For example, when the collagen-containing liquid composition is placed in a transparent PET container and stored in a place exposed to sunlight for 12 weeks, L ^* ₁ , a ^* ₁ , and b ^* ₁ are measured at week 0 (at the start of storage). It is a colorimeter value, and L ^* ₂ , a ^* ₂ , b ^* ₂ may be a colorimeter value measured at week 12, and ΔE ^* _ab may be a color difference representing the color change of the collagen-containing liquid composition over 12 weeks.

The colorimeter referred to in this specification is the “CIE-Lab colorimeter”, which is indicated by L*, a* and b* (CIE-Lab values) specified by the Commission Internationale de l'clairage (CIE). , L*, a* and b* represent the three characteristics of color: brightness, saturation and hue. Specifically, L* represents brightness and is expressed as a value from 0 to 100. The a* and b* are the plain coordinates (x, y) of the colorimetric system, where the horizontal axis is a* and the vertical axis is b*. At this time, a* is a green to red spectrum, where the +a side approaches red and the -a side approaches green, and is expressed as a value from -120 to +120. In addition, b* is a blue to yellow spectrum, with the +b side approaching yellow and the -b side approaching blue, with values ranging from -120 to +120 like a*. It is displayed as

In the present invention, the pH of the collagen-containing liquid composition may be 3 to 4. Specifically, the pH may be 3 or more, 3.2 or more, or 3.4 or more, and may be 3.6 or less, 3.8 or less, or 4.0 or less. When this pH range is satisfied, a significant discoloration effect occurs due to light shading, providing an advantageous effect in terms of stability. It can be expressed.

In the present invention, the liquid composition may further include one or more additives selected from the group consisting of acidulants, sweeteners, preservatives, anti-foaming agents, suspending agents, antioxidants, stabilizers, and fragrances.

The acidulant can be added in an appropriate amount to adjust acidity. The acidulant includes, but is not limited to, citric acid, acetic acid, tartaric acid, malic acid, lactic acid, fumaric acid, phosphoric acid, and salts thereof.

Additionally, the sweetener can be added in an appropriate amount to mask the bitter taste. The sweeteners include xylitol, D-sorbitol, maltitol, mannitol, erythritol, lactose, glucose, oligosaccharide, fructose, refined sugar (sugar), aspartame, stevioside, enzyme-treated stevia, sucralose, steviol glycoside, etc. It is not limited to this.

Additionally, the preservative may be added in an appropriate amount to improve preservability. The preservatives include, but are not limited to, dehydroacetic acid, sorbic acid, benzoic acid, propionic acid, and paraoxybenzoic acid ester.

Additionally, the antifoaming agent can be added in an appropriate amount to remove air bubbles. The antifoaming agent includes carnosic acid, lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, etc., but is not limited thereto.

Additionally, the scent may be an air freshener. The air fresheners include, but are not limited to, orange oil, fruit juice extract, cinnamon oil, spearmint oil, peppermint water, vanilla, peppermint oil, rose oil, lemon oil, berry flavor, strawberry flavor, grape flavor, berry flavor, and yogurt flavor. no.

In addition, the liquid composition of the present invention may further include additives commonly used in food compositions, such as thickeners, extenders, or pH adjusters.

In the present invention, the liquid composition may be a food composition or a health functional food composition.

Additionally, the liquid composition may be formulated into a beverage or drink agent.

In addition, even if the liquid composition is commercialized in a transparent PET container, discoloration due to external environments such as sunlight can be minimized, thereby increasing the recycling rate of the transparent PET container.

Method for producing collagen-containing liquid composition

The present invention relates to a method for producing a collagen-containing liquid composition, comprising: (i) collagen; and (ii) dissolving one or more selected from black carrot extract and prune extract, and may further include dissolving an additive. At this time, the types and contents of the collagen, black carrot extract, prune extract, and additives are as described above.

In the present invention, the black carrot extract can be prepared through the steps of grinding, extracting, and filtering black carrots, and a black carrot concentrate can be prepared by adding the step of concentrating. Alternatively, black carrot concentrate can be prepared by extracting and concentrating the black carrot. At this time, the extraction temperature may be 60°C or higher, 65°C or higher, or 70°C or higher, and may be 85°C or lower, 90°C or lower, or 95°C or lower. Additionally, the extraction time may be 0.5 hours or more or 1 hour or more, and may be 2 hours or less or 3 hours or less. Additionally, the extraction solvent may be at least one selected from the group consisting of water and alcohols having 1 to 5 carbon atoms, and preferably at least one selected from the group consisting of water and ethanol.

In the present invention, the prune extract can be prepared through the steps of grinding, extracting, and filtering the prunes, and the prune concentrate can be prepared by adding the step of concentrating. Alternatively, prune concentrate can be prepared by extracting and concentrating the prunes. At this time, the extraction temperature may be 60°C or higher, 65°C or higher, or 70°C or higher, and may be 85°C or lower, 90°C or lower, or 95°C or lower. Additionally, the extraction time may be 0.5 hours or more or 1 hour or more, and may be 2 hours or less or 3 hours or less. Additionally, the extraction solvent may be at least one selected from the group consisting of water and alcohols having 1 to 5 carbon atoms, and preferably at least one selected from the group consisting of water and ethanol.

Preferred examples are presented below to aid understanding of the present invention. However, the following examples are merely illustrative of the present invention, and it is obvious to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention. , it is natural that such changes and modifications fall within the scope of the attached patent claims.

In the following examples and comparative examples, collagen-containing liquid compositions were prepared according to the composition shown in Table 1 below.

Raw materials (unit: weight%) Example 1 Example 2 Example 3 Comparative Example 1 collagen peptides 4.00 4.00 4.00 4.00 Prune concentrate 1.50 2.00 0.00 0.00 Black carrot concentrate 0.50 0.00 2.00 0.00 incense 0.50 0.50 0.50 0.50 acidifier citric acid 0.15 0.15 0.15 0.15 sweetener xylitol 10.00 10.00 10.00 10.00 Purified water 83.35 83.35 83.35 85.35 Sum 100.00 100.00 100.00 100.00

Example 1

According to the composition as shown in Table 1, collagen peptides (AP Collagen Enzyme-Degraded Peptide, Amore Pacific Co., Ltd.), prune concentrate (Prune Concentrate, Taylor Brothers Farms) and black carrot concentrate (Black Carrot Concentrate, Nature) were mixed with purified water. After dissolving in , fragrance, citric acid, and xylitol were further dissolved to prepare a collagen-containing liquid composition.

Example 2

A collagen-containing liquid composition was prepared in the same manner as Example 1, except that black carrot concentrate was not used.

Example 3

A collagen-containing liquid composition was prepared in the same manner as Example 1, except that the prune concentrate was not used.

Comparative Example 1

A collagen-containing liquid composition was prepared in the same manner as Example 1, except that black carrot concentrate and prune concentrate were not used.

Experimental Example 1

The liquid compositions prepared in Examples and Comparative Examples were each placed in transparent PET containers and stored in a place exposed to sunlight. Using a spectrophotometer (Spectro Photometer CM-5, KONICA MINOLTA), the chromaticity (L ^* , a ^* , b ^* ) of the liquid composition was measured at 0, 2, 4, 8, and 12 weeks after storage. ) was measured.

The measured chromaticity was applied to Equation 1 below to calculate the color difference (ΔE*ab) according to storage time.

<Equation 1>

In Equation 1, L ^* ₁ , a ^* ₁ , b ^* ₁ and L ^* ₂ , a ^* ₂ , b ^* ₂ mean colorimeter values measured over a certain period of time.

Figure 1 shows the color difference of the liquid compositions prepared in Examples and Comparative Examples by storage period.

Referring to Figure 1, it can be seen that Example 1, which includes both black carrot concentrate and prune concentrate, has the least amount of change in color difference over storage time. Specifically, in Example 1, it was confirmed that the color difference was 4 or less when stored for 2 weeks, the color difference was 6 or less when stored for 4 weeks, the color difference was 6 or less when stored for 8 weeks, and the color difference was 7 or less when stored for 12 weeks.

Examples 2 and 3 are liquid compositions that do not contain black carrot concentrate and prune concentrate, respectively, compared to Example 1, and show a relatively large color difference over storage time compared to Example 1.

In addition, Comparative Example 1 was a liquid composition containing neither black carrot concentrate nor prune concentrate, and showed the largest color difference over storage time.

From these results, it was found that black carrot concentrate and prune concentrate play a role in preventing discoloration of collagen-containing liquid compositions, and in particular, when black carrot concentrate and prune concentrate are used together, the discoloration prevention effect is the best.

Although the present invention has been described above with limited examples and drawings, the present invention is not limited thereto, and the technical idea of the present invention and the following description will be provided by those skilled in the art in the technical field to which the present invention pertains. Of course, various modifications and variations are possible within the scope of equivalence of the patent claims.

Claims (11)

(i) Collagen; and (ii) at least one selected from black carrot extract and prune extract. A liquid composition containing collagen.
According to paragraph 1,
A liquid composition containing collagen, wherein the collagen is fish-derived collagen.
According to paragraph 1,
A liquid composition containing collagen, wherein the collagen has a glycine-proline-hydroxyproline (GPH) content of 2.5% by weight to 5% by weight.
According to paragraph 1,
A liquid composition containing collagen, wherein the black carrot extract is included in an amount of 0.1% to 5% by weight based on the total weight of the composition.
According to paragraph 1,
A liquid composition containing collagen, wherein the prune extract is contained in an amount of 0.1% to 5% by weight based on the total weight of the composition.
According to paragraph 1,
A liquid composition containing collagen, wherein the collagen is contained in an amount of 0.1% by weight to 10% by weight based on the total weight of the composition.
According to paragraph 1,
The color change of the composition is calculated as the color difference (ΔE ^* _ab ) defined by Equation 1 below,
A liquid composition wherein the color difference (ΔE ^* _ab ) is 12 or less:
<Equation 1>

In Equation 1, L ^* ₁ , a ^* ₁ , b ^* ₁ and L ^* ₂ , a ^* ₂ , b ^* ₂ mean colorimeter values measured over a certain period of time.
According to paragraph 1,
A liquid composition containing collagen, wherein the composition has a pH of 3 to 4.
According to paragraph 1,
The composition further includes one or more additives selected from the group consisting of acidulants, sweeteners, preservatives, antifoaming agents, suspending agents, antioxidants, stabilizers, and fragrances. Collagen-containing liquid composition.
According to paragraph 1,
The composition is a liquid composition containing collagen, which is a food composition or a health functional food composition.
According to paragraph 1,
The composition is a collagen-containing liquid composition formulated as a beverage or drink.