KR20230156576A - Cartridge for detecting target analyte capable of seling chamber - Google Patents

Abstract

The target analyte detection cartridge capable of chamber sealing according to the present invention is provided to cover one side of the chamber body, a chamber body having a plurality of chambers including a sample introduction chamber into which the sample is introduced, and one of the plurality of chambers. A cover having a chamber seal capable of sealing at least one, wherein one of the chamber body and the cover has a first position where the chamber seal opens the corresponding chamber with respect to the other, and a second position where the chamber seal opens the corresponding chamber. It can be optionally placed in position 2.

Description

Target analyte detection cartridge with chamber sealability {CARTRIDGE FOR DETECTING TARGET ANALYTE CAPABLE OF SELING CHAMBER}

The present invention relates to a target analyte detection cartridge capable of sealing the chamber.

As modern people's interest in health increases and life expectancy extends, the importance of nucleic acid-based in vitro molecular diagnosis, such as accurate analysis of pathogens and genetic analysis of patients, is increasing, and the demand for it is increasing. Nucleic acid-based molecular diagnosis is performed by extracting nucleic acids from a specimen sample and then confirming the presence or absence of target nucleic acids among the extracted nucleic acids.

Polymerase chain reaction (PCR) is the most widely used nucleic acid amplification reaction, which involves repeated cycles of denaturation of double-stranded DNA, annealing of oligonucleotide primers to DNA templates, and primer extension by DNA polymerase. Includes (Mullis et al., US Pat. Nos. 4,683,195, 4,683,202 and 4,800,159; Saiki et al., (1985) Science 230, 1350-1354).

The real-time PCR method using a fluorescent substance is a method of detecting an increase in fluorescence intensity due to nucleic acid amplification during the PCR process. The real-time PCR method has the advantage of enabling multiplex detection by using different fluorescent dyes for each target, but has the disadvantage of requiring expensive equipment and taking a long time to detect.

Meanwhile, POC (Point of Care) diagnostic technology, which accurately and quickly diagnoses a patient's disease regardless of time and place, has recently attracted attention as a very important technology for evidence-based precision medicine.

However, POC diagnostic equipment that simplifies the structure of the device and makes it smaller while reducing processing time has the problem of low detection accuracy of target substances.

Against this background, the present invention provides a target analyte detection cartridge capable of sealing a chamber, including a cover assembly capable of sealing a detection well.

In order to achieve the above object, one aspect of the present invention is a chamber body having a plurality of chambers including a sample introduction chamber into which a sample is introduced; and a cover provided to cover one side of the chamber body and including a chamber seal capable of sealing one or more of the plurality of chambers, wherein one of the chamber body and the cover is exposed to the other. , providing a target analyte detection cartridge capable of sealing the chamber, wherein the chamber sealing portion can be selectively positioned in a first position for opening the corresponding chamber and a second position for sealing the corresponding chamber.

In one embodiment of the present invention, one of the chamber body and the cover may be provided to be movable in one direction with a predetermined angle on the one surface relative to the other.

Here, one of the chamber body and the cover may be provided to be movable in a plane direction parallel to the one surface relative to the other.

Here, one of the chamber body and the cover is provided to be rotatable relative to the other, and one of the chamber body and the cover is provided to be movable in the direction of the rotation axis relative to the other. You can.

In one embodiment of the present invention, the chamber body further includes a detection chamber in which a detection well for detecting a target analyte is provided, and the chamber sealing unit includes a detection well sealing unit capable of sealing the detection well, One of the chamber body and the cover may be selectively positioned in a first position to open the detection well and a second position to close the detection well with respect to the other.

Here, the detection well sealing part may be a detection well plug capable of sealing the detection well inlet.

Here, the detection well sealing unit may be provided to seal the detection well while one of the chamber body and the cover is relatively close to the other in the direction of the rotation axis.

Here, the detection well sealing portion may be provided to extend downward from the bottom of the cover facing one side of the chamber body.

Here, the detection well sealing part may be provided so that it can be forcibly fitted into the detection well inlet.

Here, the detection well sealing part may include a detection well sealing member that is forcibly fitted into the detection well inlet and in close contact with the inner surface of the detection well.

Here, the detection well sealing member may be formed in the detection well sealing part using a double injection method.

In one embodiment of the present invention, the cover may be provided with a light transmitting portion capable of transmitting detection light to the detection well.

Here, the detection well sealing portion is provided to extend downward from the bottom of the cover facing one side of the chamber body, and the cover may be provided with a detection groove that is recessed from the upper surface in the protruding direction of the detection well sealing portion. .

Alternatively, the cover may include a peripheral sealing portion that is seated around the detection chamber while being close to the chamber body.

In one embodiment of the present invention, the detection chamber may include a guide portion having an inclined surface inclined downward in the direction of the detection well to guide the sample to the detection well.

Here, a fine pattern that reduces the surface friction coefficient may be formed in the guide portion of the detection chamber.

Alternatively, the guide portion of the detection chamber may be formed with a guide protrusion that guides the sample to the center of the width direction.

Alternatively, either the chamber body or the cover may be provided to be rotatable relative to the other, and the guide portion of the detection chamber may be located closer to the center of the chamber body than the detection well.

In one embodiment of the present invention, the chamber body includes a plurality of detection chambers arranged in parallel with each other, and the detection chambers have a straight line having a predetermined angle in a radial direction of the chamber body for the detection. It can be arranged to pass through wells.

Here, the detection chamber is provided in the form of a long hole extending in a direction parallel to one of the radial directions, and the detection well may be located outside the guide portion in the radial direction.

According to one embodiment of the present invention, leakage of the chambers can be prevented through the cover while also facilitating suction and discharge of samples or reagents.

In addition, gas leakage during the detection process can be prevented by allowing the detection well sealing portion provided on the cover to change its relative position with respect to the detection well.

The effects of the present invention are not limited to the effects described above, and should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

Figure 1 is a perspective view showing a target analyte detection cartridge according to one embodiment.
Figure 2 is an exploded perspective view of Figure 1.
FIG. 3 is a view showing FIG. 2 viewed from below.
Figure 4 is a perspective view showing a chamber body on which a sealing member is mounted.
Figure 5 is a perspective view of the chamber body.
Figure 6 is a top view of the chamber body.
Figure 7 is a plan view showing a transparent cover at the sample injection location.
Figure 8 is a plan view showing a transparent cover at the sample detection position.
Figure 9 is a cross-sectional view at the sample detection position.
Figure 10 is a perspective view showing a sample injection chamber.
Figure 11 is a cross-sectional view showing the sample injection chamber at the sample injection position.
Figure 12 is a cross-sectional view showing the sample injection chamber at the sample discharge position.
Figure 13 is a plan view showing the beadwell.
Figure 14 is a perspective cross-sectional view showing a beadwell.
Figure 15 is a plan view showing the detection chamber.
Figure 16 is a cross-sectional view of the detection line in Figure 15.
Figure 17 is a cross-sectional view showing the detection chamber.
Figure 18 is a side view showing the rotation prevention structure.
Figure 19 is a cross-sectional view showing the separation prevention structure.
Figure 20 is a cross-sectional view showing the sealing ring.

Hereinafter, the present invention will be described in detail through examples and illustrative drawings. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention.

Additionally, when adding reference numerals to components in each drawing, it should be noted that the same components are given the same reference numerals as much as possible even if they are shown in different drawings. Additionally, when describing the present invention, if it is determined that a detailed description of a related known configuration or function may obscure the gist of the present invention, the detailed description will be omitted.

Additionally, when describing the components of the present invention, terms such as first, second, A, B, (a), (b), (i), or (ii) may be used. These terms are only used to distinguish the component from other components, and the nature, order, or order of the component is not limited by the term. When a component is described as being "connected," "coupled," or "connected" to another component, that component may be directly connected or connected to the other component, but there is another component between each component. can be understood as being able to be “connected,” “coupled,” or “connected.”

The present invention relates to a detection device for detecting target analytes in a sample.

As used herein, “sample” may include biological samples (e.g., cells, tissues, and fluids from biological sources) and non-biological samples (e.g., food, water, and soil). The biological samples include viruses, bacteria, tissues, cells, blood (e.g. whole blood, plasma and serum), lymph, bone marrow fluid, saliva, sputum, swabs, aspiration, milk and urine. , feces, ocular fluid, semen, brain extract, spinal fluid, joint fluid, thymic fluid, bronchial lavage fluid, ascites, and amniotic fluid. Additionally, the sample may include natural and synthetic nucleic acid molecules isolated from biological sources. According to one embodiment of the present invention, the sample may include additional substances such as water, deionized water, saline solution, pH buffer solution, acidic solution, and basic solution.

A target analyte refers to an analyte that is subject to analysis. The analysis may mean, for example, obtaining information about the presence, content, concentration, sequence, activity or characteristics of the analyte in the sample. Analytes can include a variety of substances (e.g., biological substances and non-biological substances such as compounds). Specifically, the analytes may include biological substances such as nucleic acid molecules (e.g., DNA and RNA), proteins, peptides, carbohydrates, lipids, amino acids, biological compounds, hormones, antibodies, antigens, metabolites, and cells. there is. According to one embodiment of the present invention, the analyte may be a nucleic acid molecule.

Therefore, the target analyte detection device of the present invention may be a target nucleic acid detection device. The target nucleic acid detection device allows the nucleic acid reaction in the sample to proceed and detects the target nucleic acid through this.

Nucleic acid reaction refers to a series of physical and chemical reactions that generate a signal depending on the presence or amount of nucleic acid of a specific sequence in a sample. The nucleic acid reaction may be a reaction including binding of a nucleic acid of a specific sequence in a sample to another nucleic acid or substance, or replication, cleavage, or decomposition of a nucleic acid of a specific sequence in the sample. The nucleic acid reaction may be a reaction involving a nucleic acid amplification reaction. The nucleic acid amplification reaction may include amplification of a target nucleic acid. The nucleic acid amplification reaction may be a reaction that specifically amplifies a target nucleic acid.

The nucleic acid reaction may be a signal-generating reaction, which is a reaction capable of generating a signal depending on the presence/absence or amount of the target nucleic acid in the sample. This signal-generating reaction may be a genetic analysis process such as PCR, real-time PCR, or microarray.

Various methods are known for generating an optical signal indicating the presence of a target nucleic acid using a nucleic acid reaction. Representative examples include: the TaqManTM probe method (U.S. Patent No. 5,210,015), the molecular beacon method (Tyagi et al., Nature Biotechnology v.14 MARCH 1996), the Scorpion method (Whitcombe et al., Nature Biotechnology 17:804- 807 (1999)), Sunrise or Amplifluor method (Nazarenko et al., 2516-2521 Nucleic Acids Research, 25(12):2516-2521 (1997), and US Patent No. 6,117,635), Lux method (US Patent No. 7,537,886), CPT (Duck P, et al. Biotechniques, 9:142-148 (1990)), LNA method (US Patent No. 6,977,295), Plexor method (Sherrill CB, et al., Journal of the American Chemical Society, 126:4550-4556 (2004)), HybeaconsTM (D. J. French, et al., Molecular and Cellular Probes (2001) 13, 363-374 and U.S. Patent No. 7,348,141), double-labeled self- Quenched probe (Dual-labeled, self-quenched probe; U.S. Patent No. 5,876,930), hybridization probe (Bernard PS, et al., Clin Chem 2000, 46, 147-148), PTOCE (PTO cleavage and extension) method ( WO 2012/096523), PCE-SH (PTO Cleavage and Extension-Dependent Signaling Oligonucleotide Hybridization) method (WO 2013/115442), PCE-NH (PTO Cleavage and Extension-Dependent Non-Hybridization) method (PCT/KR2013/012312) and CER method (WO 2011/037306).

The target analyte detection device according to an embodiment of the present invention may be a nucleic acid detection device and can detect a signal that occurs depending on the presence of a target nucleic acid. A nucleic acid detection device can detect a signal by amplifying it along with nucleic acid amplification. Alternatively, the nucleic acid detection device may detect the signal by amplifying the signal without amplifying the nucleic acid. Preferably, the signal is detected accompanied by nucleic acid amplification.

A target analyte detection device according to an embodiment of the present invention may include a nucleic acid amplification device.

A nucleic acid amplification device refers to a device that can perform a nucleic acid amplification reaction to amplify a nucleic acid having a specific nucleotide sequence. Methods for amplifying the nucleic acids include the polymerase chain reaction (PCR) and ligase chain reaction (LCR) (U.S. Patents Nos. 4,683,195 and 4,683,202; PCR Protocols: A) Guide to Methods and Applications (Innis et al., eds, 1990), strand displacement amplification (SDA) (Walker, et al. Nucleic Acids Res. 20(7):1691-6 (1992); Walker PCR Methods Appl 3(1):1-6 (1993)), transcription-mediated amplification (Phyffer, et al., J. Clin. Microbiol. 34:834-841 (1996); Vuorinen, et al., J. Clin. Microbiol. 33:1856-1859 (1995)), nucleic acid sequence-based amplification (NASBA) (Compton, Nature 350(6313):91-2 (1991) ), rolling circle amplification (RCA) (Lisby, Mol. Biotechnol. 12(1):75-99 (1999); Hatch et al., Genet. Anal. 15(2):35-40 (1999) )) and Q-Beta Replicase (Lizardi et al., BiolTechnology 6:1197 (1988)).

The target analyte detection device according to one embodiment may be a device that performs a nucleic acid amplification reaction while changing temperature. For example, to amplify DNA (deoxyribonucleic acid) with a specific base sequence, a nucleic acid amplification device can perform a denaturing step, an annealing step, and an extension (or amplification) step. there is.

The denaturation step is a step in which double-stranded DNA is separated into single-stranded DNA by heating a solution containing a sample containing double-stranded DNA, which is a template nucleic acid, and a reagent to a specific temperature, for example, about 95°C. The annealing step provides an oligonucleotide primer having a nucleotide sequence complementary to the nucleotide sequence of the nucleic acid to be amplified, cools the separated single-stranded DNA to a specific temperature, for example, 60°C, and amplifies the single-stranded DNA. This is the step of forming a partial DNA-primer complex by binding a primer to a specific nucleotide sequence. In the extension step, after the annealing step, the solution is maintained at a specific temperature, for example, 72°C, and a double-stranded DNA is formed based on the primer of the partial DNA-primer complex by DNA polymerase. do.

By repeating the three steps described above, for example, 10 to 50 times, DNA having the specific nucleotide sequence can be amplified exponentially. In some cases, the nucleic acid amplification device may perform the annealing step and the extension step simultaneously. In this case, the nucleic acid amplification device may complete the first cycle by performing two steps consisting of a denaturation step and an annealing/extension step.

In particular, the target analyte detection device according to one embodiment may be a device that performs a nucleic acid amplification reaction and a reaction that generates an optical signal depending on the presence of the nucleic acid while accompanied by a change in temperature and detects the generated optical signal.

Additionally, the target analyte detection device according to one embodiment may include an optical module, a thermal module, a control unit for controlling them, a frame supporting the optical module and the thermal module, a case surrounding them, and a display unit.

The optical module consists of an optical housing that forms a dark room, a light source that generates excitation light, an excitation light filter unit that can provide the wavelength band of the target analyte, a beam splitter that can set the optical path, and a reaction container (e.g. For example, it may include a heat lead that pressurizes and heats the cover of the tube, and a detection unit that detects the emission light emitted from the sample.

The excitation light filter unit may include an excitation light filter wheel provided with a plurality of filters having different wavelength bands, and a driver that drives the excitation light filter wheel.

The detection unit may include a detection sensor that acquires an image from the emitted light, an emitted light filter unit, and an emitted light lens array that controls the path and focus of the light.

The emission light filter unit may include an emission light filter wheel provided with a plurality of filters having different wavelength bands, and a driving unit that drives the emission light filter wheel.

The thermal module may include a thermal block in which a plurality of wells for accommodating a reaction vessel are formed, a thermal unit including a thermal element, a cooling unit for cooling the thermal block, and a thermal block housing.

The cooling unit may include a heat dissipation plate and a cooling fan.

Next, the operation of the target analyte detection device according to an embodiment will be described.

The light source emits light to excite the fluorescent substances contained in the sample. For example, the light source may be an LED (Light Emitting Diode).

The light emitted by the light source may be displayed as an excitation beam, and the light emitted by the sample may be displayed as an emission beam. And the path of the excitation beam emitted from the light source may be expressed as an excitation path, and the path of the emission beam emitted from the sample may be expressed as an emission path.

A beam splitter can selectively reflect or transmit incident light. The excitation light from the light source passes through the beam splitter, passes through the hole of the heat lead, and reaches the reaction vessel accommodated in the thermal block.

Then, the light emitted from the sample is reflected by the beam splitter, passes through the emission light lens array, and reaches the detection sensor.

The thermal block may be a thermally conductive material. When the thermal block contacts the reaction vessels, heat may be transferred from the thermal block to the reaction vessels. For example, thermal blocks can be made of metals such as aluminum, gold, silver, nickel, or copper.

The thermal block housing can accommodate a thermal block, thermal element, and cooling unit inside.

The thermal element can increase or decrease the temperature of the thermal block. The thermal element is disposed below the thermal block and can transfer heat to the thermal block or absorb heat from the thermal block by contacting the thermal block. As an example, the thermal element may be a Peltier element or a heat wire, and may include an FPCB board to which they are connected.

The cooling unit may include a heat sink or heat sink that is disposed below the thermal block and radiates to the outside of the thermal block. Additionally, the cooling unit may include a cooling fan that provides external air to cool the heat sink or heat sink fins.

The detection unit detects a signal from the sample. Specifically, the detection unit includes a detection sensor that detects fluorescence generated from the sample. The detection sensor may be provided as a charge coupled device (CCD), complementary metal oxide semiconductor field effect transistor (CMOS), or photodiode.

A target analyte detection device according to an embodiment includes a chamber body, a light-emitting module, and a detection module.

A sample refers to a substance for which the target analyte is to be detected. The samples include biological samples (e.g., cells, tissues, and fluids from biological sources) and non-biological samples (e.g., food, water, and soil). Biological samples include viruses, bacteria, tissues, cells, blood, serum, plasma, lymph, sputum, swab, aspiration, bronchoalveolar lavage fluid, milk, urine, feces, ocular fluid, saliva, Including, but not limited to, semen, brain extract, spinal fluid (SCF), appendix, spleen and tonsil tissue extract, amniotic fluid and ascites. Additionally, the sample may include natural and synthetic nucleic acid molecules isolated from biological sources.

In the present invention, the sample may contain substances necessary for detecting the target analyte. For example, the sample may include additional substances such as water, deionized water, saline solution, pH buffer, acidic solution, basic solution. Additionally, according to one embodiment of the present invention, the sample may include an optical label. The optical label refers to a label that generates an optical signal depending on the presence of a target nucleic acid. The optical label may be a fluorescent label. The fluorescent label useful in the present invention may include any molecule known in the art.

The light emitting module according to the present invention supplies appropriate optical stimulation to the sample accommodated in the chamber body, and the detection module detects the optical signal generated from the sample in response to this.

The optical signal may be luminescence, phosphorescence, chemiluminescence, fluorescence, polarized fluorescence, or other colored signal. The optical signal may be an optical signal generated in response to optical stimulation applied to the sample.

The chamber body is formed as a chamber to accommodate samples, reagents, or beads. That is, the chamber body is a component that directly accommodates a sample, reagent, or bead in the chamber or a reaction vessel containing the sample, reagent, or bead.

As used herein, the expression “the chamber body can receive a sample” can be used to generically refer to cases where the chamber body receives a sample directly in the chamber or a reaction vessel containing the sample.

The chamber body positions the sample at a predetermined position so that optical stimulation from the light emitting module reaches the sample and an optical signal generated from the sample reaches the detection module.

Heat may be supplied to the chamber body by a heat generating element, and heat may be transferred to a sample accommodated directly in the chamber body or a sample accommodated in a reaction vessel.

Reaction vessels can be made of a variety of materials, such as plastic, ceramic, glass, or metal.

The chamber body accommodating the reaction vessel may have the shape of a block or plate. The chamber body accommodating the reaction vessel may include a recess, for example a well, or have a flat surface. The chamber body accommodating the reaction vessel may have a structure that guides the position of the reaction vessel or fixes the sample reaction vessel.

One chamber body is configured to accommodate one or more samples.

A representative example of a chamber body that accommodates a reaction vessel is a thermal block. The thermal block includes a plurality of wells or holes, in which reaction vessels may be accommodated.

That the chamber body accommodates the sample reaction containers may mean that the sample reaction containers are placed in a plurality of wells formed in the chamber body or in an assigned position on the chamber body.

The reaction vessel is used to accommodate the sample to be analyzed, and may be of various types, such as a tube, a vial, a strip connected to a plurality of single tubes, or a plate connected to a plurality of tubes. Includes a plate, microcard, chip, cuvette, or cartridge.

The chamber body that directly accommodates the sample may have the shape of the reaction vessel described above and may be formed of the material of the reaction vessel described above.

In one embodiment, the chamber body may be formed of a thermally conductive material. When the chamber body is in direct contact with the sample or reaction vessels, heat may be transferred from the chamber body to the sample or the sample within the reaction vessel.

The chamber body may be made of metal such as aluminum, gold, silver, nickel or copper, or may be made of plastic or ceramic.

In this way, the chamber body is formed to accommodate a plurality of samples, and the temperature of the plurality of samples is adjusted to enable a reaction for detection, such as a nucleic acid amplification reaction, to occur. For example, when the chamber body is a thermal block in which a plurality of wells are formed, the chamber body is formed of one thermal block, and all wells of the thermal block may be formed not to be thermally independent from each other. In this case, the temperatures of all wells in the chamber body where samples are received are the same, and the temperatures of the received samples cannot be adjusted according to different protocols.

As another example, the chamber body may be configured to control the temperature of some of the samples accommodated in the chamber body according to different protocols. In other words, the chamber body may include two or more thermally independent reaction regions. Each reaction zone is thermally independent. Heat does not transfer from one reaction zone to another. For example, there may be an insulating material or an air gap between the reaction regions. The temperature of each reaction zone can be controlled independently. A reaction protocol including temperature and time can be individually set for each of the reaction zones, and each of the reaction zones can perform the reaction according to an independent protocol. Since the reaction proceeds according to an independent protocol in the reaction regions, the time points of light detection in the reaction regions are independent of each other.

With reference to FIGS. 1 to 18 , a cartridge 10 according to an embodiment of the present invention will be described.

The cartridge 10 according to an embodiment of the present invention may include a chamber body 100 that can accommodate samples and reagents, and a cover 200 that covers the top of the chamber body 100. It may further include a pipette tip 400 coupled to an end portion of the pipette that can suction and discharge fluids such as samples or reagents accommodated in the chamber body 100.

The chamber body 100 may be provided with a plurality of chambers. The chambers may have an opening formed on the upper surface of the chamber body 100 and a fluid receiving portion formed on the lower part.

The cover 200 may be provided to face all or part of the upper surface of the chamber body 100. Additionally, the cover 200 may be provided to cover all or part of the outer side of the chamber body 100. For example, when the chamber body 100 is provided in a cylindrical shape having an upper surface and an outer surface, the cover 200 has a cover surface covering the upper surface of the chamber body 100 and the chamber body 100. ) may include a side surrounding the outer surface of the.

Additionally, the cover 200 can prevent substances contained in the chamber from escaping to the outside of the cartridge 10 and prevent foreign substances outside the cartridge 10 from entering the chamber. Furthermore, the cover 200 may prevent fluid or gas in the chamber from escaping to the outside. In particular, while a reaction occurs in the detection well 152, which will be described later, the cover 200 seals the detection well 152 to prevent external contaminants from flowing into the inside, and at the same time prevents the reaction substances in the detection well 152 from entering. It is possible to prevent leakage to the outside, and further prevent the gas in the detection well 152 from escaping to the outside. For this purpose, the target analyte detection device may be provided with a separate pressurizing unit (not shown) that pressurizes the cover 200 or the chamber body 100.

Meanwhile, one of the chamber body 100 and the cover 200 may be provided to be movable in a plane direction parallel to the one surface relative to the other.

In this embodiment, the cover 200 may be provided to move up and down relative to the chamber body 100. Being able to relatively move up and down means that the cover 200 can move and the chamber body 100 can move. And the vertical movement includes not only vertical movement but also movement in a direction at a predetermined angle to the vertical direction. And the vertical movement may include moving to a close position and moving to a touching position.

For example, the cover 200 may move downward. The cover surface of the cover 200 is in contact with the upper surface of the chamber body 100, or the portion protruding downward from the cover surface of the cover 200 is the chamber of the chamber body 100 or the chamber body 100. ), the cover 200 can seal one or more of the chambers when lowered to contact the periphery of the chamber.

Additionally, one of the chamber body 100 and the cover 200 may be provided to be movable in one direction with a predetermined angle on one surface relative to the other.

In this embodiment, the chamber body 100 may be provided to be rotatably movable relative to the cover 200. Being able to rotate relatively means that the cover 200 can rotate and the chamber body 100 can rotate.

For example, the cover 200 may be provided in a fixed state, and the chamber body 100 may be provided to rotate.

More specifically, the chamber body 100 is provided to be rotatable about a rotation axis, and the cover 200 surrounds the outer surface of the chamber body 100 and allows the chamber body 100 to rotate. It can be provided. For example, the chamber body 100 may have a circular shape when viewed from the top, and the cover 200 may include an inner surface provided to surround the outer surface of the chamber body 100.

The chamber body 100 may rotate in one direction or in both directions based on the central axis. For example, it can be rotated about 360 degrees in one direction, or it can be rotated about 180 degrees clockwise and about 180 degrees counterclockwise. In addition, various rotation angles and directions may be provided depending on the design of the chamber body 100.

The rotational driving force of the chamber body 100 may be transmitted through the lower part of the chamber body 100. For this purpose, the target analyte detection device may be equipped with a separate rotation drive unit (not shown) that rotates the chamber body 100. For example, the chamber body 100 may be coupled to the drive shaft of the rotation drive unit at the center.

According to the basic embodiment described below, the chamber body 100 may be provided to be rotatable and the cover 200 may be provided to be movable up and down. However, unlike this, it is also possible for the cover 200 to be rotatable and the chamber body 100 to be movable up and down. Alternatively, various embodiments are possible, such as the chamber body 100 or the cover 200 being provided to enable both rotational movement and vertical movement.

Referring to FIG. 9, the chamber body 100 may include a mounting portion 160 into which a drive shaft of a rotation drive unit (not shown) can be inserted around the center of the rotation axis. The mounting portion 160 may include a collar portion 161 extending upward from the lower surface of the chamber body 100 along the central axis. Alternatively, the collar portion 161 may extend downward from the upper surface of the chamber body 100 along the central axis.

Additionally, the mounting portion 160 may include a connecting groove 162 that accommodates the connecting rod 240 extending downward around the central axis of the cover 200. For example, the connecting groove 162 may be provided on the outside of the collar portion 161. The connecting rod 140 is guided to the connecting groove 162 and can move up and down inside the connecting groove 162.

Or, conversely, it is also possible for a connecting groove to be formed in the cover 200 and a connecting rod to be provided in the chamber body 100.

Meanwhile, the target analyte detection device includes a pipette (not shown) that provides suction pressure and discharge pressure. As an example, the pipette may be connected to a pump (not shown) that provides pneumatic pressure.

The cartridge 10 may be provided with a pipette tip 400 coupled to the end of the pipette. The pipette tip 400 moves integrally with the pipette and can transmit the pneumatic pressure of the pipette to the fluid accommodated in the chamber of the chamber body 100.

Additionally, the pipette tip 400 may be coupled to the cover 200 to allow up and down movement. To this end, the cover 200 may form a pipette hole 211 through which the pipette or pipette tip 400 can enter and exit.

The chamber body 100 may be allowed to rotate while the lower end of the pipette tip 400 is located above the upper surface of the chamber body 100. The control unit that controls the rotational drive of the target analyte detection cartridge 10 may align the position of the chamber body 100 so that the pipette tip 400 is located at the upper part of the target chamber. Therefore, the pipette discharges fluid from all chambers of the chamber body 100 or all chambers of the chamber body 100 through the pipette tip 400 by only moving up and down without moving in the plane direction, including revolution. Fluid can be injected.

The chamber body 100 may include a plurality of chambers arranged in a circumferential direction with respect to the central axis of rotation. Some of the plurality of chambers may have a shape extending in the radial direction of the chamber body 100. For example, some of the plurality of chambers may be provided in the shape of a long hole with a radial length longer than a circumferential width.

Additionally, some of the plurality of chambers may have a shape whose cross-sectional area becomes narrower toward the bottom. For example, some of the plurality of chambers may have a shape in which the circumferential width narrows toward the bottom. Alternatively, some of the plurality of chambers may have a shape in which the radial width narrows toward the bottom.

Additionally, referring to FIG. 4, the cartridge 10 according to an embodiment of the present invention may further include a sealing member 300 that covers the openings of the plurality of chambers exposed at the top of the chamber body 100. . The sealing member 300 may be provided to seal the entrance of the chamber after inserting reagents, buffers, or beads into the chambers.

The sealing member 300 may be a thin membrane, for example, aluminum foil (Al foil). And the sealing member 300 may be attached to the upper surface of the chamber body 100 using heat fusion or adhesive.

The sealing member 300 is provided so that it can be damaged by the tip of the pipette tip 400. Alternatively, when a chamber insertion part (not shown) protruding from the lower surface of the cover 200 is provided, the sealing member 300 may be damaged by pressure applied by the chamber insertion part as the cover 200 descends. It may be possible. The sealing protrusion may descend while tearing the sealing member 300 and be accommodated inside the chamber. In some cases, the sealing member 300 may be provided with a dot or line groove in the area to be cut in advance to facilitate damage.

Referring to FIG. 12, the pipette tip 400 may be coupled to the cover 200 to move up and down. A pipette may be coupled to the upper part of the pipette tip 400, and the pipette tip 400 may transmit negative pressure or positive pressure provided by the pipette.

And the tip of the pipette tip 400 may be provided in the shape of a thin tube. The tip of the pipette tip 400 can easily penetrate the sealing member 300, and fine volume control is easy.

The pipette tip 400 includes a pipette coupling portion 401 to which the pipette is coupled, a pipette tip body 402 extending downward from the pipette coupling portion 401 and forming a flow path, and the pipette tip body 402. ) may include a pipette tip tip 403 extending downward, and a pipette tip filter member 404 provided in the middle of the flow path inside the pipette tip body 402.

The cover 200 may further include a pipette tip guide 210 that guides the up and down movement of the pipette tip 400. The pipette tip guide 210 may extend along the movement path of the pipette tip 400. For example, the pipette tip guide 210 is provided to protrude above the upper surface of the cover 200 and the pipette tip 400 can be accommodated therein. Since the cover 200 approaches or comes into close contact with the chamber body 100 when detecting a target analyte, there may be insufficient space under the upper surface of the cover 200 for the pipette tip guide 210 to extend.

And the pipette hole 211 formed in the cover 200 may be provided inside the pipette tip guide 210. For example, the pipette hole 211 may be formed to penetrate the upper surface of the cover 200. You can. The pipette tip 400 can enter and exit through the pipette hole 211.

And the pipette tip 400 is provided so that the outer surface includes a cylinder or a partial cylindrical shape, and the pipette tip guide 210 has an inner surface including a cylinder or a partial cylindrical shape corresponding to the outer surface of the pipette tip 400. It can be arranged to do so. Additionally, the inner diameter of the pipette tip guide 210 is slightly larger than the outer diameter of the pipette tip 400 to reduce friction between the pipette tip guide 210 and the pipette tip 400.

In addition, the pipette tip guide 210 may be provided with a support jaw 212 for supporting the pipette tip 400 to prevent the pipette tip 400 from coming off downward, and the pipette tip 400 may be provided with a support jaw 212. It may be provided with a first stopping protrusion 405 that protrudes outward to be supported by (212).

For example, the first stopping protrusion 405 may have a flange shape that protrudes outward from the outer surface. And with the cover 200 spaced apart from the chamber body 100, when the first stopping protrusion 405 of the pipette tip 400 is supported on the pipette tip guide 210, the pipette tip ( The distal end of 400) may be spaced apart from the bottom of the chamber. Therefore, when the pipette tip 400 moves downward, the pipette tip tip can be prevented from being damaged by colliding with the bottom of the chamber.

Additionally, the chambers of the chamber body 100 may have the same depth, and the pipette tip tip 403, which is provided in a tubular shape, may be provided to be equal to or longer than the depth of the chambers. Accordingly, when the pipette tip tip 403 penetrates the sealing member 300 that covers the upper part of the chambers, the sealing member 300 can be prevented from being damaged more than necessary.

A pipette tip filter member 404 may be further included inside the pipette tip 400. The pipette tip filter member 404 can prevent contaminants from outside air from flowing into the cartridge 10. The pipette tip filter member 404 may be located inside the pipette tip body 402, and sufficient volume may be secured below the pipette tip filter member 404. Therefore, when the pipette tip 400 sucks fluid from the chamber, the fluid may not contact the pipette tip filter member 404. This is because, if the fluid sucked through the pipette tip tip 403 contacts the pipette tip filter member 404, it may be exposed to contaminants filtered by the pipette tip filter member 404.

Additionally, a second locking protrusion 406 may be provided on the outer surface of the pipette tip 400 to prevent the pipette tip 400 from moving upward. The second stopping protrusion 406 of the pipette tip 400 may protrude in a ring shape along the outer surface of the pipette tip 400.

And the second stopping protrusion 406 may seal the space between the pipette tip 400 and the side wall of the pipette tip guide 210. The second stopping protrusion 406 may be formed integrally with the pipette tip 400, or may be made of a separate elastic material. And the second stopping protrusion 406 may be formed on the pipette tip 400 using a double injection method.

And the inner surface of the pipette tip guide 210 may include a stepped structure. The step structure may be provided so that the lower inner diameter is larger than the upper inner diameter. The upper inner diameter of the pipette tip guide 210 may be larger than the outer diameter of the body of the pipette tip 400 and smaller than the outer diameter of the second stopping protrusion 406. Additionally, the lower inner diameter of the pipette tip guide 210 may be provided to correspond to the outer diameter of the second stopping protrusion 406 of the pipette tip 400.

Additionally, the second stopping protrusion 406 of the pipette tip 400 may be provided to enable elastic deformation. Therefore, the pipette tip 400 can be assembled through the upper part of the pipette tip guide 210 in an interference fit manner, and the second stopping protrusion 406 passes through the step structure of the pipette tip guide 210. Afterwards, it is elastically restored and can be prevented from coming off again.

Referring to FIG. 6, the target analyte cartridge 10 includes a sample injection chamber 110 into which a sample is injected, and a first bead chamber 120a into which protease K (proteinase K) beads are accommodated. ), a second bead chamber (120b) in which magnetic beads are accommodated, a third bead chamber (120c) in which internal control beads are accommodated, and a lie in which a lysis buffer (lysis buffer) is accommodated. A sheath buffer chamber (130a), a tip mounting chamber (142) where the tip is mounted, a mixing chamber (141) where fluids are mixed (mixed), and a binding buffer chamber where the binding buffer is accommodated. It may include (130b), a plurality of buffer chambers (130c, 130d, 130e) in which the washing buffer is accommodated, and an extra dummy chamber (143). The above chambers may be arranged in a circumferential direction (eg, clockwise) in the above order.

In addition, the target analyte cartridge 10 may further include a plurality of detection chambers 150 for detecting the target analyte contained in the sample arranged linearly, along with chambers provided in the circumferential direction.

Meanwhile, when the distance between the center of rotation C and the pipette tip 400 is the pipette radius Rp, a plurality of chambers may be provided to include the pipette radius Rp therein. For example, a plurality of chambers excluding the detection chambers 150 may be provided so that the center of each chamber is located at the pipette radius (Rp). Alternatively, the plurality of detection chambers 150 may have different distances from the rotation center C to the center of each chamber.

Referring to FIG. 15 , the detection chambers 150 may be arranged so that a straight line Ld having a predetermined angle to the radial direction Dr of the chamber body 100 passes through the detection chambers 150 . Additionally, the plurality of detection chambers 150 may be arranged side by side at equal intervals in a certain straight direction (Ld).

This is because the detection module (not shown) of the target analyte detection device is provided to move linearly, so that the plurality of detection chambers 150 are located on the travel path (Ld) of the detection module.

For example, the distance between the center of each detection chamber 150 and the center of rotation (C) is the shortest, and the distance between the center of the detection chamber 150 located in the center and the center of rotation (C) is the shortest, and the distance between the center of each detection chamber 150 and the center of rotation (C) is the shortest, and the distance between the center of each detection chamber 150 and the center of rotation (C) is the shortest, and the distance between the center of each detection chamber 150 and the center of rotation (C) is the shortest. The distance between the center of the chamber 150 and the center of rotation (C) may be decreased or increased to the greatest distance.

Meanwhile, referring to FIG. 9, the cover 200 has a sample injection hole 221 formed to enable sample injection into the sample injection chamber 110, and a stopper 220 that seals the sample injection hole 221. may include. And the stopper 220 is integrally connected with the cover 200 to prevent loss.

And the cover 200 may further include a sample guide portion 222 extending downward from the sample injection hole 221. The sample guide unit 222 can block the surrounding area so that the sample provided through the sample injection hole 221 can flow into the sample injection chamber 110. For example, the sample guide unit 222 may be provided in a cylindrical shape, and may be provided with a shape and width to enable sample injection into the sample injection chamber 110.

Meanwhile, referring to FIG. 6, the sample injection chamber 110 may be provided in the form of a long hole extending in the circumferential direction. The sample injection chamber 110 may have a different location at which the sample is injected (hereinafter referred to as sample injection area 111) and a location at which the sample is discharged (hereinafter referred to as sample discharge area 112). For example, in the sample injection chamber 110, the center of the sample injection area 111 and the center of the sample discharge area 112 may be arranged to be offset by a predetermined angle at the same radial distance based on the rotation center C. Additionally, the center of the sample injection area 111 and the center of the sample discharge area 112 may be defined as the pipette radius (Rp).

Referring to FIGS. 9 to 12 , the sample injection chamber 110 may be spatially divided into a sample injection area 111 and a sample discharge area 112. By distinguishing the area 111 where the sample is injected into the sample injection chamber 110 and the area 112 where the sample is discharged, suspended matter or contaminants that may be included in the sample injected into the cartridge 10 are prevented from being discharged through the pipette. It can be prevented in the first place.

Additionally, the sample injection chamber 110 may be provided with a blocking portion that spatially blocks the space between the sample injection area 111 and the sample discharge area 112. For example, the blocking unit may be a blocking wall 113.

Additionally, the sample injection chamber 110 may be provided with a sample flow channel 114 penetrating the barrier wall 113. For example, the blocking wall 113 may be provided in a cylindrical shape, and the sample flow channel 114 may be a through hole penetrating the lower part of the blocking wall 113. Additionally, the sample flow channel 114 is formed to penetrate both opposing sides of the barrier wall 113, thereby reducing sample flow resistance.

In addition, the height of the blocking wall 113 is provided higher than when the sample injection chamber 110 is full of samples, so that movement of the sample except through the sample flow channel 114 can be restricted. In addition, the sample flow channel 114 is provided close to the bottom of the sample injection chamber 110 to minimize the amount of sample remaining in the sample injection area 111 without moving to the sample discharge area 112. there is.

That is, the sample inside the sample injection area 111 can flow into the sample discharge area 112 provided inside the blocking wall 113 only through the sample flow channel 114. And the pipette can discharge the sample from the sample discharge area 112. In this way, by providing the blocking wall 113 in the sample injection chamber 110, it is possible to secondarily prevent contaminants other than those floating on the bottom from being discharged.

Additionally, the sample injection chamber 110 may further include a sample filter 115. The sample filter 115 may be installed in the sample discharge area 112 or the sample flow channel 114. And the pipette tip 400 that discharges the sample from the sample discharge area 112 can discharge the sample located on the upper part of the sample filter 115 that has been filtered while passing through the sample filter 115.

Specifically, the sample injected into the sample injection area 111 of the sample injection chamber 110 may pass through the sample flow channel 114 and the sample filter 115 and move to the sample discharge area 112. . At this time, suspended matter or contaminants in the sample can be finally removed while passing through the sample filter 115. Meanwhile, the sample filter 115 can be removed or replaced as needed.

Additionally, the sample filter 115 may be installed below the blocking wall 113 and may be installed to separate the upper and lower parts of the blocking wall 113. For example, the sample filter 115 may be provided at the lower portion of the cylindrical blocking wall 113 and may be provided to shield the entrance to the sample discharge area 112. Alternatively, the sample filter 115 may be provided in the sample flow channel 114. Alternatively, the sample filter 115 may be installed in the space between the bottom surface of the sample injection chamber 110 and the blocking wall 113.

Meanwhile, the sample flow channel 114 may be provided adjacent to the bottom of the sample injection chamber 110. And the sample flow channel 114 may be provided at the boundary between the sample injection area 111 and the sample discharge area 112. For example, the sample flow channel 114 may be a flow path that penetrates the barrier wall 113. Specifically, the flow channel 114 may be a hole penetrating the lower part of the cylindrical blocking wall 113.

And the sample flow channel 114 may be provided to penetrate both sides of the blocking wall 113. Therefore, the flow resistance of the sample flowing through the sample flow channel 114 can be reduced.

And the sample filter 115 may be located above the sample flow channel 114. For example, the sample filter 115 may be installed on the inner side of the cylindrical blocking wall 113 and on top of the sample flow channel 114.

Additionally, the blocking wall 113 may be provided as a separate member from the sample injection chamber 110. And the blocking wall 113 can be detachably coupled to the sample injection chamber 110. For example, the sample discharge area 112 of the sample injection chamber 110 may be provided with a blocking wall fixture that protrudes upward from the bottom surface. And the blocking wall 113 can be fixed by coupling to the blocking wall fixing part. For example, the blocking wall 113 is provided in a cylindrical shape, and the blocking wall fixing part may be a cylinder or a partial shape of a cylinder capable of accommodating the blocking wall 113 therein. Additionally, a sample flow channel 114 may be formed in the barrier wall 113 and/or the barrier wall fixture. For example, the sample flow channel 114 may be a hole penetrating the barrier wall 113 and/or the barrier wall fixture.

Additionally, the sample injection chamber 110 may include a step 116 structure in which the internal cross-sectional area is drastically different between the upper region and the lower region. The step 116 may be provided in the sample injection area 111. For example, the step 116 may be provided on a side of the sample injection chamber 110 facing the sample discharge area 112.

And the upper and lower walls of the step 116 may include an inclined structure. And the step 116 may also include an inclined structure. At this time, the slope of the surface of the step 116 may be smaller than the slope of the upper and lower surfaces of the step 116. The inclined structure can guide the sample to the bottom of the sample injection chamber 110 despite the viscosity of the injected sample.

The lower area of the sample injection chamber 110 may correspond to the volume of a single sample. And the upper area of the sample injection chamber 110 may correspond to the volume of a plurality of samples for sample pooling test. For example, when performing a test by collecting five single samples, the samples may fill beyond the lower area of the sample injection chamber 110 to the upper area.

In this way, the sample injection chamber 110 includes a step 116 structure and an inclined structure, so that it has a volume capable of responding to a sample pooling test and secures a sufficient height for the sample to be discharged even during a single sample test. You can. If the lower cross-sectional area of the sample injection chamber 110 is provided to be large, the height of the sample is lowered when examining a single sample, and the sample volume discharged by the pipette tip 400 from the sample discharge area 112 may not be sufficient. .

Meanwhile, when injecting a sample, the cover 200 has the sample injection hole 221 located above the sample injection area 111, and when detecting a sample, the sample injection hole 221 is located above the sample discharge area 112. ) can be located above.

And the cover 200 may be provided so that the sample injection hole 221 and the sample guide part 222 are positioned on the blocking wall 113 when detecting a sample. For example, the sample guide part 222 and the blocking wall 113 are provided in cylindrical shapes with different radii, and the sample guide part 222 can accommodate the blocking wall 113 therein. It can be provided with a radius. Accordingly, when the cover 200 approaches the chamber body 100 when detecting a sample, the blocking wall 113 enters the sample guide portion 222 and interference between them can be prevented.

Meanwhile, referring to Figures 13 and 14, the bead chamber 120 can accommodate beads 122 (or freeze-drying reagent) therein. And the internal width of the bead chamber 120 can be determined by considering the size of the bead 122. If the internal width of the bead chamber 120 is too large than the size of the bead 122, the free movement of the bead 122 increases, and there is a risk that the bead 122 may be damaged during packaging or transportation or become attached to the inner wall of the chamber due to frictional static electricity. Because this exists.

And the depth of the bead chamber 120 can be determined considering the size of the bead 122. If the bead 122 is placed adjacent to the upper surface of the bead chamber 120, the bead 122 may be affected by heat during the heating process for foil sealing of the bead chamber 120. Therefore, the bead chamber 120 may be provided relatively deeper than the size of the bead 122.

And the width and height of the bead chamber 120 may be determined depending on the size of the bead 122. Specifically, the width of the bead chamber 120 may be determined in the range of 150% to 200% of the size of the bead 122, and the depth of the bead chamber 120 may be 200% of the size of the bead 122. It can be determined in the range of % to 300%. For example, when the size of the bead is 2.5 mm, the circumferential width of the bead chamber 120 may be 4.5 mm, the radial length may be 9.5 mm, and the height may be 6.5 mm.

Additionally, a bump protrusion 121 may be provided at the bottom of the area where the pipette tip 400 descends in the bead chamber 120 to prevent the bead 122 from being damaged. The bump protrusion 121 may be provided in a shape that prevents the bead 122 from being seated on the bump protrusion 121. For example, the bump protrusion 121 is wider than the width of the bead 122. It includes a small width, and the bump protrusion 121 may be a low protrusion extending in the radial direction. This is because, if the bead 122 is located in an area where the pipette tip 400 descends in the absence of the bump protrusion 121, the bead 122 may be damaged by the distal end of the pipette tip 400.

Meanwhile, referring to FIG. 15, the detection chambers 150 may have chambers of the same shape arranged at regular intervals in a straight direction (Ld) perpendicular to the radial reflection (Dr) of the chamber body 100. . Additionally, the detection chambers 150 may be in the form of long holes extending in a direction parallel to the radial direction Dr connecting the center of rotation C at the central point of the detection chambers 150.

For example, when the detection chambers 150 are provided in an odd number, the detection chambers 150 are located at the center with respect to the detection chamber 150 located at the center among the detection chambers 150. It may be provided in the form of a long hole extending in a direction parallel to the radial direction of (150). Additionally, the detection chambers 150 located on the left and right sides of the detection chamber 150 located in the center may be arranged at regular intervals in a straight line perpendicular to the radial direction.

As another example, when the detection chambers 150 are provided in an even number, the detection chambers 150 are positioned at the center based on the center points of the two detection chambers 150 located in the center among the detection chambers 150. It may be provided in the form of a long hole extending in a direction parallel to the radial direction of the point. Additionally, the detection chambers 150 located on the left and right sides of the two centrally located detection chambers 150 may be arranged at regular intervals in a straight line perpendicular to the radial direction.

In addition, referring to FIG. 6, the length of the long hole of the detection chamber 150 is determined by the location of the distal end of the pipette tip 400 within the long hole when the pipette tip 400 is located at the upper part of the corresponding detection chamber 150. It can be arranged to include on the side. Alternatively, the length of the long hole of the detection chambers 150 may be set such that the radial distance (Rp) from the rotation center to the tip of the pipette tip 400 is included inside all of the plurality of detection chambers 150. You can. Here, the distal end of the pipette tip 400 being included in the inner side means when viewed from the planar direction, and the distal end of the pipette tip 400 may also be located at the upper part of the detection chambers 150 when viewed from the side. there is.

And when viewed in a plan direction, the pipette tip 400 is located on the outside of the pipette tip 400 in the radial direction of the long hole in one or more detection chambers 150 located in the center, and the detection chambers 150 located on the outside are located on the inside of the radial direction of the long hole. The pipette tip 400 can be positioned in . That is, as the plurality of detection chambers 150 move from the center to the outside based on the center of the direction in which they are arranged, the position at which the sample is injected from the pipette tip 400 may move from the outside to the inside in the radial direction of the long hole.

Referring again to FIG. 15, the detection area where the sample injected into each detection chamber 150 is detected through the photodetector must be located on the same line (Ld). That is, as the plurality of detection chambers 150 move from the center to the outside based on the center of the direction in which the plurality of detection chambers 150 are arranged, the injection area where the sample is injected from the pipette tip 400 and the detection area become distant from each other, and the detection chamber located in the center (150) may have the same injection area and detection area.

Additionally, one or more of the plurality of detection chambers 150 may be arranged side by side in the travel direction (Ld) of the photodetector or in a direction parallel thereto. In addition, one or more of the plurality of detection chambers 150 may be arranged at equal intervals in the travel direction (Ld) of the photodetector or in a direction parallel thereto.

Additionally, the plurality of detection chambers 150 may have a long hole shape extending in a direction perpendicular to the travel direction (Ld) of the photodetector or a direction parallel thereto. For example, in a long hole shape, an injection area may be provided at a position close to the radial direction, and a detection area may be provided at a position far from the radial direction. However, in some cases, the injection area and radius area may be the same.

And the detection area may be provided deeper than the injection area. Additionally, the injection area may include an inclined surface 151 whose depth increases in the direction of the detection area, and the detection area may include a detection well 152 which is recessed deeper than the inclined surface 151. The sample injected into the injection area may move along the inclined surface 151 and be accommodated inside the detection well 152. In addition, the boundary between the inclined surface 151 and the detection well 152 is provided with a sharp discontinuity to prevent backflow of the sample.

Additionally, a fine pattern including a water-repellent function may be formed on the inclined surface 151. For example, a micropattern may be formed with a size of about 100 micrometers. Therefore, the entire sample injected into the inclined surface 151 can flow into the detection well 152 along a fine pattern with low friction resistance.

This is because differences in detection results may occur depending on differences in the capacity of the sample accommodated in the detection chamber 150. That is, in order to improve detection accuracy, the same volume of sample must be accommodated in the plurality of detection wells 152.

Additionally, a guide portion 153 that guides the sample to the detection well 152 may be provided on the inclined surface 151. For example, the guide portion 153 may be a guide protrusion formed on both sides of the area where the sample flows. The distance between the two guide portions 153 may be prepared to include a decreasing portion in the direction of sample flow.

Meanwhile, beads (not shown) can be accommodated in the detection well 152. For example, freeze-dried beads may be accommodated in the detection well 152. Additionally, a bead jamming prevention protrusion (not shown) may be provided between the injection area or the injection area and the detection area. For example, the bead jamming prevention protrusion may be provided on the inclined surface 117 to limit the movement of the bead to prevent the bead from getting caught on the shallow side of the inclined surface 117. For example, the bead jamming prevention bump may be provided in a protrusion or rib shape, and the guide portion 153 may be the bead jamming prevention bump.

Meanwhile, the cover 200 may be partially transparent to light, or may be entirely transparent to light. The cover 200 allows the excitation light emitted from the light emitter located at the top to reach the sample accommodated in the detection well 152, and at the same time allows the reflected light emitted from the optical label of the sample to reach the photodetector. It is permissible. That is, the cover 200 may be provided so that the paths of the excitation light and the reflected light are light transparent.

Meanwhile, the target analyte detection cartridge 10 according to an embodiment of the present invention may include a chamber sealing unit capable of sealing one or more chambers among a plurality of chambers. For example, the cover 200 may have a shape corresponding to the chamber formed in the chamber body 100 and may include a chamber sealing portion that protrudes downward toward the chamber.

The chamber seal opens the corresponding chamber when the cover 200 is spaced apart from the chamber body 100, but when the cover 200 descends and approaches or comes into close contact with the chamber body 100, it is inserted into the chamber or is prevented from doing so. When fitted, the chamber can be airtight or sealed.

Additionally, when a plurality of chambers are arranged side by side, the chamber seals may be provided side by side depending on the shape and arrangement of the plurality of chambers.

In this way, by providing a chamber seal, it is possible to prevent safety and environmental problems that may occur after the target analyte detection cartridge 10 has been used. If the use of the target analyte detection cartridge 10 is terminated and the user unmounts and discards the cartridge 10 from the detection device, reagents or gases inside the chamber leak to the outside of the cartridge 10. It may harm the safety or health/sanitation of the product and may cause environmental pollution even after disposal.

However, this risk can be prevented by sealing the chamber of the target analyte detection cartridge 10.

Referring to FIGS. 16 and 17 , the cover 200 may further include a detection well plug 230 that seals the detection well 152 of the detection chamber 150. The detection well plug 230 can prevent the sample in the detection well 152 from leaking out of the detection well 152, and further, gas is generated while the sample reacts in the detection well 152. It can support increasing pressure while doing so.

For example, the cover 200 forms a detection well plug 230 that protrudes downward, and the detection well plug 230 can seal the detection well 152 of the detection chamber 150. . For example, the detection well 152 may be provided as a cylindrical groove, and the detection well plug 230 may be provided as a cylindrical protrusion inserted into the inside of the detection well 152. At this time, the detection well plug 230 may be press-fitted to the detection well 152.

Additionally, a sealing member 231 may be provided on the outer surface of the detection well plug 230. The sealing member 231 may be provided on the outer surface of the lower end of the protrusion of the detection well plug 230. The sealing member 231 may be made of a material capable of elastic deformation. For example, the sealing member may be formed on the outer surface of the detection well plug 230 using a double injection method.

As the cover 200 descends, the detection well plug 230 is press-fitted into the detection well 152, and the inside of the detection well 152 can be blocked from the outside. In addition, the detection well plug 230 can withstand an increase in pressure caused by gas generated inside the detection well 152 due to frictional force acting between the detection well plug 230 and the inner wall of the detection well 152. It can be arranged so that Alternatively, by providing downward pressure to the cover 200, the pressure inside the detection well 152 increases, causing the detection well plug 230 to break away from the detection well 152 or move inside the detection well 152. It can prevent gas from leaking.

In addition, the cover 200 may further include a detection groove 232 recessed into the upper part of the detection well plug 230. By forming the detection groove 232 on the top of the detection well plug 230, the length of the optical path passing through the cover 200 can be reduced. For example, the detection well plug 230 is provided in a cylindrical shape with an outer diameter corresponding to the inner diameter of the detection well 152, and the detection well plug 230 is located on the upper part of the detection well plug 230. The detection groove 232, which is cylindrical and has an inner diameter smaller than the outer diameter, may be recessed.

Additionally, the cover 200 may further include a peripheral sealing portion 234 that contacts the upper surface of the chamber body 100 around the detection chamber 150. The peripheral sealing portion 234 may be provided on the lower surface of the cover 200 and around the detection well plug 230. And the peripheral sealing part 234 may contact the surroundings of the detection chamber 150 to seal the detection chamber 150. For example, the peripheral sealing portion 234 may be provided in a ring shape to accommodate the detection chamber 150 therein.

Alternatively, the peripheral sealing portion 234 may be formed on the upper surface of the chamber body 100 rather than on the lower surface of the cover 200.

[Up and down guide structure]

Meanwhile, referring to FIGS. 1 to 9 , the cover 200 may be allowed to move downward only at a certain position of the chamber body 100.

The cover 200 may be rotated away from the upper surface of the chamber body 100 in the sample preparation step before detecting the target material, but in the target material detection step, the detection well plug 230 is connected to the detection well 152. ) While aligned above, the detection well plug 230 comes down to approach the upper surface of the chamber body 100 and seals the detection well 152.

However, when the cover 200 is lowered toward the chamber body 100 while the detection well plug 230 is not aligned on the detection well 152, there is a gap between the cover 200 and the chamber body 100. Interference occurs.

To this end, the cover 200 and the chamber body 100 may include a vertical guide structure. The upper and lower guide structures may include protrusions and grooves.

The cover 200 has upper and lower guide protrusions 250 on its inner side, and the chamber body 100 has upper and lower guide grooves on its outer side that extend in the vertical direction to guide the upper and lower guide protrusions 250 in the vertical direction. 170) can be provided.

On the contrary, the chamber body 100 has upper and lower guide protrusions on the outer side, and the cover 200 extends in the vertical direction to guide the upper and lower guide protrusions of the chamber body 100 on the inner side in the vertical direction. Up and down guide grooves may be provided.

Alternatively, the vertical guide structure may include a protrusion formed on the lower surface of the cover 200 and a groove formed on the upper surface of the chamber body 100. In this case, the cover 200 may be allowed to descend only when the protrusions and grooves are aligned with each other as the cover 200 rotates.

In addition, two or more upper and lower guide structures may be provided in the rotational direction or circumferential direction. Therefore, distortion can be prevented during up and down movement and durability can be improved. For example, four vertical guide structures may be provided at 90-degree intervals in the rotation direction, or three may be provided at 120-degree intervals.

Additionally, the plurality of upper and lower guide structures may be provided at different intervals. For example, three upper and lower guide structures can be provided at intervals of 130 degrees and 110 degrees, respectively. In this case, the alignment of the upper and lower guide structures can be correct only at one position. Accordingly, the cover 200 can be allowed to descend only when the detection well plug 230 is aligned on the detection well 152 in the target material detection step.

[Anti-rotation structure]

Meanwhile, it is necessary to prevent the user from arbitrarily rotating the cover 200 before the target analyte detection cartridge 10 is mounted on the detection device.

In the initial state of the product, the alignment of the upper and lower guide structures may be misaligned. Therefore, it is possible to prevent the user from arbitrarily pressing the cover. However, it is necessary to prevent the user from arbitrarily rotating the cover and manipulating the upper and lower guide structures in an aligned state.

In addition, in the initial state of the cartridge 10 product, the pipette tip 400 may be provided in a state accommodated in the space of the tip mounting chamber 142. In this case, when the user arbitrarily rotates the cover 200, the pipette tip 400 Damage may occur.

And if the cover 200 is allowed to rotate arbitrarily before the cartridge 10 is inserted into the detection device, the cover 200 is allowed to descend relative to the chamber body 100 at the point where the upper and lower guide structures match, thereby allowing the chamber body ( This is because the sealing member 300 that seals the chambers of 100 may be damaged.

To this end, the cover 200 and the chamber body 100 may include a rotation prevention structure. The anti-rotation structure may include protrusions and grooves.

Referring to FIG. 18, the cover 200 has a rotation prevention protrusion 253 at the bottom when it is on the inside, and the chamber body 100 has a rotation prevention protrusion 253 at the top when it is on the outside. A prevention groove 173 may be provided. On the contrary, if the chamber body 100 is on the outside, it may be provided with an anti-rotation protrusion at the top, and if it is on the inside, the cover 200 may be provided with an anti-rotation groove at the bottom for receiving the anti-rotation protrusion.

Additionally, the anti-rotation protrusion 253 may be provided to enable elastic deformation in the radial direction. For example, the rotation prevention protrusion 253 may have a cantilever shape extending downward of the cover 200. Alternatively, incisions may be formed on both sides of the anti-rotation protrusion 253. Therefore, when the cover 200 is rotated above a certain torque, the anti-rotation protrusion 253 may move outward in the radial direction and leave the anti-rotation groove 173. After the rotation prevention protrusion 253 leaves the rotation prevention groove 173, the cover 200 may be rotatable on the upper part of the chamber body 100.

[Press-resistant structure]

Meanwhile, the cover 200 may be prevented from moving downward with respect to the chamber body 100 below a certain pressure. This is because the cover 200 is allowed to descend relative to the chamber body 100 at the point where the upper and lower guide structures are aligned in the sample preparation stage, which may cause the sealing member 300 that seals the chambers of the chamber body 100 to be damaged.

To this end, the cover 200 and the chamber body 100 may include a push-prevention structure. The anti-press structure may include protrusions and locking protrusions.

For example, the cover 200 is provided with an anti-press protrusion (not shown) that protrudes outward in the radial direction from the lower part of the connecting rod 240, and is provided at the upper part of the connecting groove 162 of the chamber body 100. An anti-pressure jaw (not shown) that protrudes inward in the radial direction may be provided.

Additionally, the anti-press protrusion may be provided to enable elastic deformation in the radial direction. For example, the anti-press protrusion may have a cantilever shape extending downward of the cover 200. Alternatively, incisions may be formed on both sides of the anti-press protrusion. Therefore, when the cover 200 is pressed with a certain pressure or more, the anti-press protrusion may move inward in the radial direction and leave the anti-press protrusion. After the anti-press protrusion leaves the anti-press ledge, the cover 200 may move downward with respect to the chamber body 100.

[Escape prevention structure]

Meanwhile, the cover 200 can be prevented from being separated upward from the chamber body 100. This is because if the user arbitrarily allows the cover 200 to be separated from the chamber body 100, the sealing member 300 that seals the chambers of the chamber body 100 may be damaged.

To this end, the cover 200 and the chamber body 100 may include a separation prevention structure. The breakaway prevention structure may include protrusions and locking protrusions.

Referring to FIG. 19, the cover 200 is provided with a first separation prevention protrusion 251 that protrudes radially inward from the lower portion of the side portion, and the chamber body 100 protrudes radially outward from the upper portion of the side portion. A first separation prevention jaw 171 may be provided.

The movement of the cover 200 is restricted in the upward direction because the first separation prevention protrusion 251 is caught by the first separation prevention jaw 171, but in the downward direction, the first separation prevention protrusion 251 chamber body 100 Downward movement along the outer side may be permitted.

In addition, the cover 200 is provided with a second separation prevention protrusion 252 that protrudes radially outward from the lower part of the connecting rod 240, and the chamber body 100 has a radial protrusion at the upper part of the connecting groove 162. A second separation prevention protrusion 172 may be provided that protrudes inward.

The movement of the cover 200 is restricted in the upward direction because the second separation prevention protrusion 252 is caught by the second separation prevention jaw 172, but in the downward direction, the second separation prevention protrusion 252 is connected to the connecting groove 162. ) may be allowed to move downward along the inner surface of the

[Sealing ring]

Meanwhile, referring to FIGS. 19 and 20 , the cover 200 or the chamber body 100 may further include a sealing ring 174 that blocks the chambers of the chamber body 100 from external air.

For example, the sealing ring 174 may be provided on the outer surface of the chamber body 100 and may be provided in a ring shape surrounding the chamber body 100 in the circumferential direction. In addition, the sealing ring 174 can block air outside the cartridge 10 while contacting the inner side of the cover 200.

Alternatively, the sealing ring 174 may be provided on the inner surface of the cover 200 and may be provided in a ring shape surrounding the chamber body 100 in the circumferential direction. In addition, the sealing ring 174 can block air outside the cartridge 10 while contacting the outer side of the chamber body 100.

And the sealing ring 174 may be located below the upper and lower guide grooves 170 at the lower part of the chamber body 100. Because it is located below the upper and lower guide grooves 170, it can be provided in a continuous ring shape on the outer surface of the chamber body 100.

In the sample preparation step, when the cover 200 is separated from the upper surface of the chamber body 100, the sealing ring 174 may be provided without contacting the inner surface of the cover 200. Accordingly, the cover 200 or the chamber body 100 can be allowed to rotate freely.

And in the target analyte detection step, when the cover 200 approaches or comes into close contact with the chamber body 100, the sealing ring 174 may be in contact with the inner surface of the cover 200. Therefore, the cover 200 can block the outside air of the cartridge 10.

Alternatively, the sealing ring 174 may be provided on the upper outer surface of the chamber body 100 or on the lower inner surface of the cover 200. In this case, the sealing ring 174 may always be interposed between the chamber body 100 and the cover 200 regardless of the position of the cover 200. At this time, the sealing ring 174 may have a friction coefficient sufficient to allow free rotation of the chamber body 100 or the cover 200.

And the sealing ring 174 is provided as a separate member and combined with the cover 200 or the chamber body 100, or is formed by double injection molding on the cover 200 or the chamber body 100. can be formed.

Next, a method of using the target analyte detection device and the target analyte detection cartridge 10 according to an embodiment of the present invention will be described.

The target analyte detection cartridge 10 is provided in a packaged state. The user unpacks and takes out the target analyte detection cartridge 10. Then, the stopper 220 of the cover 200 can be opened and the sample can be injected with a pipette through the sample injection hole 221. In the initial state, the sample injection hole 221 is located above the sample injection area 111 of the sample injection chamber 110, so the entire sample is injected into the sample injection area 111. The user closes the stopper 220 of the cover 200 after injecting the sample.

Then, the user mounts the cartridge 10 on the target analyte detection device. For example, the robot arm of the target analyte detection device may move outside the device, and the user may place the target analyte detection device on it. Alternatively, the user may install the cartridge 10 by inserting it into a slot of the target analyte detection device.

At this time, the cartridge 10 may be mounted so that the cover 200 is positioned above and the chamber body 100 is positioned below.

Afterwards, the robot arm moves inside the device so that the center of the cartridge 10 is aligned with the drive shaft of the rotation drive unit. Then, the cartridge 10 or the drive shaft is moved to couple the cartridge 10 to the drive shaft. For example, as the robot arm puts down the cartridge 10, the drive shaft may be inserted and coupled to the inside of the collar portion 161 of the coupling mounting portion 160.

Next, the detection device moves the pipette to couple the end of the pipette to the pipette tip 400. The pipette tip 400 of the cartridge 10 is located at the top of the tip mounting chamber 142 in its initial state. The detection device may restrain the cover 200 from rotating during the coupling process of the pipette and the pipette tip 400. For example, when the detection device moves the pipette downward and presses the pipette tip 400, the first stopping protrusion 405 of the pipette tip 400 is supported on the support jaw 212 of the pipette tip guide 210. , In this state, pressure is applied to the pipette tip 400 and it is coupled. And before the chamber body 100 rotates, the pipette is raised so that the tip of the pipette tip 400 is positioned above the upper surface of the chamber body 100.

Meanwhile, referring to FIG. 6, the tip mounting chamber 142 may be provided in the form of a long hole extending in the circumferential direction. The tip mounting chamber 142 can accommodate the pipette tip tip 403 in the initial state of the cartridge 10, that is, the position where the sample is injected, and the pipette tip tip 403 in the detection state of the cartridge 10 ( 403) is prepared to accommodate this. For example, the position of the tip of the pipette tip when a sample is injected and the position of the tip of the pipette tip when the target analyte is detected may be arranged to be offset by a predetermined angle at the same radial distance based on the center of rotation (C).

Next, the driving unit rotates the chamber body 100 in one direction by a predetermined angle. When the chamber body 100 rotates at a predetermined angle, the tip of the pipette tip 400 is positioned on the sample discharge area 112 of the sample injection chamber 110. Then, the pipette is moved downward so that the tip of the pipette tip 400 enters the inside of the blocking wall 113 and is submerged in the sample, and then negative pressure is provided to the pipette to discharge the sample. At this time, the sample discharged is a sample purified while passing through the sample filter 115.

Next, the driving unit rotates the chamber body 100 in one direction by a predetermined angle. When the chamber body 100 rotates at a predetermined angle, the tip of the pipette tip 400 is positioned on top of the target chamber. Then, the pipette moves downward so that the pipette tip tip 403 penetrates the sealing member 300 and enters the target chamber. Additionally, the sample or reagent can be sucked in by applying negative pressure to the pipette, or the sample or reagent can be discharged by applying positive pressure.

As the chamber body 100 rotates, the pipette tip 400 can be positioned on the target chamber, and a series of sample preparation steps can be performed while the pipette tip 400 inhales and discharges the sample or reagent. For example, the pipette may inhale the buffer from one buffer chamber 130 and discharge the buffer into one bead chamber 120. And when the beads are dissolved in the buffer, the pipette can suck them back in and discharge them into the mixing chamber 141. The sample preparation step is completed by repeating this series of processes.

And in this process, the detection device may include a heating unit capable of transferring heat to one chamber. For example, the heating unit of the detection device may transfer heat to the bead chamber 120 to promote dissolution of the beads, and transfer heat to the mixing chamber 141 to promote mixing of the sample and reagent, A detection reaction can be caused by transferring heat to the well 152.

Next, the driving unit rotates the chamber body 100 in one direction by a predetermined angle. When the chamber body 100 rotates at a predetermined angle, the tip of the pipette tip 400 may be positioned on the injection area of the detection chamber 150. Then, the pipette moves downward so that the pipette tip tip 403 penetrates the sealing member 300 and enters the detection chamber 150. Then, positive pressure is applied to the pipette to eject the sample into the injection area. The sample moves from the injection area to the detection area along the inclined surface 117 and is consequently received in the detection chamber 150.

Here, the injection area and detection area of the detection chamber 150 may be different from each other or may be the same. And the injection area of the detection chamber 150 may be an inclined surface 117 or a detection well 152. If the detection chamber 150 directly injects a sample into the detection well 152, the injection area and the detection area may refer to the same area.

Next, the driving unit rotates the chamber body 100 in one direction by a predetermined angle. When the chamber body 100 rotates at a predetermined angle, the detection well plug 230 may be positioned on the upper part of the detection well 152. In this state, the upper and lower guide structures are aligned so that the cover 200 can be allowed to move downward toward the chamber body 100. Specifically, the upper and lower guide protrusions 250 may be located at the upper part of the upper and lower guide grooves.

Next, the detection device applies more than a certain pressure to the cover 200 and pushes it down. Then, the detection well plug 230 of the cover 200 ruptures the sealing member 300 and is seated on the inner wall of the detection well 152. At this time, when the anti-press structure is applied to the cartridge 10, the anti-press protrusion is deformed by pressure and passes through the anti-press ledge, and the cover 200 may move downward.

Additionally, the detection device may provide additional pressure to the cover 200 to maintain the state in which the detection well plug 230 applies pressure to the detection well 152. When heat is applied to the detection well 152 and a detection reaction occurs, gas is generated. The gas generated at this time may increase the pressure inside the detection well 152. The detection device may prevent gas inside the detection well 152 from escaping out of the detection well 152 by applying force to press the cover 200.

And the detection device drives the detection module in the direction in which the detection chambers 150 are placed. That is, the detection module detects a plurality of detection chambers 150 while moving in a straight line. At this time, even when the detection module is provided in a stationary state and the cartridge 10 rotates in one direction, the detection module can detect a plurality of detection chambers 150. However, fluid flow occurs inside the detection well 152 due to the centrifugal force of the cartridge 10, and as a result, it may be difficult to expect accurate detection results.

The above description is merely an illustrative explanation of the technical idea of the present invention, and those skilled in the art will be able to make various modifications and variations without departing from the essential quality of the present invention. Accordingly, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but rather to explain it, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention shall be interpreted in accordance with the claims below, and all technical ideas within the scope equivalent thereto shall be construed as being included in the scope of rights of the present invention.

10: target analyte detection cartridge, 100: chamber body,
110: sample injection chamber, 111: sample injection area,
112: sample discharge area, 113: barrier,
114: sample flow channel, 115: sample filter,
116: step, 117: slope,
120: bead chamber, 121: bump projection,
122: bead, 130: buffer chamber,
141: mixing chamber, 142: tip mounting chamber,
143: dummy chamber, 150: detection chamber,
151: slope, 152: detection well,
153: guide part, 160: mounting part,
161: Collar part, 162: Connecting groove,
170: Upper and lower guide groove, 171: First separation prevention jaw,
172: second anti-separation jaw, 173: anti-rotation groove,
174: sealing ring, 200: cover,
210: pipette tip guide, 211: pipette hole,
212: support jaw, 220: stopper,
221: sample injection hole, 222: sample guide unit,
230: detection well plug, 231: sealing member,
232: detection groove, 233: light transmission part,
234: peripheral sealing part, 240: connecting rod,
250: upper and lower guide projections, 251: first separation prevention projection,
252: second anti-separation protrusion, 253: anti-rotation protrusion,
300: sealing member, 400: pipette tip,
401: pipette coupling portion, 402: pipette tip body,
403: pipette tip tip, 404: pipette tip filter member,
405: first stumbling protrusion, 406: second stumbling protrusion.

Claims (20)

A chamber body having a plurality of chambers including a sample introduction chamber into which a sample is introduced; and
A cover provided to cover one side of the chamber body and having a chamber seal capable of sealing one or more of the plurality of chambers,
One of the chamber body and the cover relative to the other,
wherein the chamber seal is selectively positioned in a first position to open the corresponding chamber and a second position to close the corresponding chamber,
Target analyte detection cartridge with chamber sealability. According to claim 1,
One of the chamber body and the cover is provided to be movable in one direction with a certain angle to the one surface relative to the other,
Target analyte detection cartridge with chamber sealability. According to clause 2,
One of the chamber body and the cover is provided to be movable in a plane direction parallel to the one surface relative to the other,
Target analyte detection cartridge with chamber sealability. According to clause 3,
One of the chamber body and the cover is provided to be rotatable relative to the other,
One of the chamber body and the cover is provided to be movable in the direction of the rotation axis relative to the other,
Target analyte detection cartridge with chamber sealability. According to any one of claims 1 to 4,
The chamber body further includes a detection chamber provided with a detection well for detecting the target analyte,
The chamber sealing portion includes a detection well sealing portion capable of sealing the detection well,
One of the chamber body and the cover relative to the other,
The detection well seal may be selectively positioned at a first position to open the detection well and a second position to close the detection well,
Target analyte detection cartridge with chamber sealability. According to clause 5,
The detection well sealing unit is a detection well plug-in capable of sealing the detection well entrance,
Target analyte detection cartridge with chamber sealability. According to clause 6,
The detection well sealing part,
One of the chamber body and the cover is provided to seal the detection well while being relatively close to the other in the direction of the rotation axis,
Target analyte detection cartridge with chamber sealability. According to clause 7,
The detection well sealing portion is provided to extend downward from the bottom of the cover facing one side of the chamber body,
Target analyte detection cartridge with chamber sealability. According to clause 8,
The detection well seal is provided so that it can be forcefully fitted into the detection well inlet,
Target analyte detection cartridge with chamber sealability. According to clause 9,
The detection well sealing part includes a detection well sealing member that is tightly fitted to the detection well inlet and is in close contact with the inner surface of the detection well.
Target analyte detection cartridge with chamber sealability. According to claim 10,
The detection well sealing member is formed in the detection well sealing part by a double injection method,
Target analyte detection cartridge with chamber sealability. According to clause 5,
The cover has a light transmitting portion capable of transmitting detection light to the detection well,
Target analyte detection cartridge with chamber sealability. According to claim 12,
The detection well sealing portion is provided to extend downward from the bottom of the cover facing one side of the chamber body,
The cover is provided with a detection groove that is recessed in the protruding direction of the detection well seal on the upper surface,
Target analyte detection cartridge with chamber sealability. According to clause 5,
The cover includes a peripheral sealing portion that is seated around the detection chamber while being close to the chamber body.
Target analyte detection cartridge with chamber sealability. According to clause 5,
The detection chamber includes a guide portion having an inclined surface inclined downward in the direction of the detection well to guide the sample to the detection well,
Target analyte detection cartridge with chamber sealability. According to claim 15,
The guide portion of the detection chamber is formed with a fine pattern that reduces the surface friction coefficient,
Target analyte detection cartridge with chamber sealability. According to claim 15,
The guide portion of the detection chamber is formed with a guide protrusion that guides the sample to the center of the width direction,
Target analyte detection cartridge with chamber sealability. According to claim 15,
Either the chamber body or the cover is provided to be rotatable relative to the other,
The guide portion of the detection chamber is located closer to the center of the chamber body than the detection well,
Target analyte detection cartridge with chamber sealability. According to clause 5,
The detection chambers are arranged in parallel with each other, and a straight line having a certain angle in a radial direction of the chamber body is arranged to pass through the detection wells,
Target analyte detection cartridge with chamber sealability. According to clause 19,
The detection chamber includes a guide portion having an inclined surface inclined downward in the direction of the detection well to guide the sample to the detection well, and is provided in the form of a long hole extending in a direction parallel to one of the radial directions,
The detection well is located outside the guide in the radial direction,
Target analyte detection cartridge with chamber sealability.

Images