KR20240041310A - Apparatus for detecting target analyte including TIR lens - Google Patents

Abstract

The target analyte detection device according to the present invention includes a sample holder configured to accommodate a sample, a light emitting module configured to irradiate excitation light to the sample accommodated in the sample holder, and emission light emitted from the sample. It includes a detection module configured to detect, and the light emitting module includes a light source element that generates excitation light, and a TIR (Total Internal Reflection) lens that guides the excitation light.

Description

Target analyte detection device including TIR lens {Apparatus for detecting target analyte including TIR lens}

The present invention relates to a target analyte detection device including a TIR lens.

As modern people's interest in health increases and life expectancy extends, the importance of nucleic acid-based in vitro molecular diagnosis, such as accurate analysis of pathogens and genetic analysis of patients, is increasing, and the demand for it is increasing. Nucleic acid-based molecular diagnosis is performed by extracting nucleic acids from a specimen sample and then confirming the presence or absence of target nucleic acids among the extracted nucleic acids.

Polymerase chain reaction (PCR) is the most widely used nucleic acid amplification reaction, which involves repeated cycles of denaturation of double-stranded DNA, annealing of oligonucleotide primers to DNA templates, and primer extension by DNA polymerase. Includes (Mullis et al., US Pat. Nos. 4,683,195, 4,683,202 and 4,800,159; Saiki et al., (1985) Science 230, 1350-1354).

In a device for detecting target analytes such as nucleic acids, a light element irradiates excitation light to samples, and optical labels contained in the samples excited by the excitation light emit optical signals. And a photodetector is configured to detect the emission light emitted from the optical label. In such an optical signal detection device, it is necessary to accurately provide excitation light to the sample and accurately provide emission light to the photodetector. Typically, the emission light emitted from the sample is guided to a photodetector by a beam splitter. That is, the excitation light irradiated from the light source device passes through the beam splitter and is provided to the sample, and the emission light emitted from the sample is reflected by the beam splitter and provided to the photodetector.

At this time, there are often cases where the signal-to-noise ratio (SNR) of the photodetector is low and detection accuracy is reduced. Therefore, various attempts are being made to improve detection accuracy by increasing the signal-to-noise ratio (SNR) of the photodetector.

Against this background, the present invention provides a target analyte detection device including a TIR lens.

In order to achieve the above object, one aspect of the present invention is a sample holder formed to accommodate a sample, a light emitting module formed to irradiate excitation light to the sample accommodated in the sample holder, and Target analysis comprising a detection module configured to detect emitted emission light, wherein the light emission module includes a light source element that generates excitation light, and a TIR (Total Internal Reflection) lens that guides the excitation light. A water detection device is provided.

In one embodiment of the present invention, the light emitting module is disposed spaced apart from the TIR lens and includes an excitation light filter formed to allow the excitation light guided by the TIR lens to pass through, detecting the target analyte. It could be a device.

In one embodiment of the present invention, the TIR lens may be a target analyte detection device that is formed to have a beam angle (θ _a ) of 16° to 24°.

In one embodiment of the present invention, the TIR lens may be a target analyte detection device that is formed to have a beam angle (θ _a ) of 18° to 22°.

In one embodiment of the present invention, the target analyte detection device may include one or more objective lenses disposed along the optical axis on top of the sample holder to guide the excitation light in parallel.

In one embodiment of the present invention, the objective lens may be a target analyte detection device, characterized in that two objective lenses are arranged along the optical axis.

In one embodiment of the present invention, the angle (θ _b ) between the light element and the objective lens may be a target analyte detection device, characterized in that it is 10° to 15°.

In one embodiment of the present invention, the light element, the TIR lens, the objective lens, and the sample receiving portion of the sample holder are arranged on the same optical axis, and the excitation light generated from the light element is generated by the TIR lens, It may be a target analyte detection device, characterized in that irradiation is sequentially passed through the objective lens and into the sample receiving portion of the sample holder.

In one embodiment of the present invention, the beam angle (θ _a ) of the TIR lens and the angle (θ _b ) between the light element and the objective lens satisfy the equation: 0.55 ≤ θ _b /θ _a ≤ 0.75. Characterized by a target analyte detection device.

In one embodiment of the present invention, the sample holder is divided into a plurality of sample areas, a plurality of sample receiving portions are formed in each area, and the light emitting module includes a plurality of light element elements, each light element element It may be a target analyte detection device, characterized in that it is formed to irradiate excitation light to each sample area.

In one embodiment of the present invention, the detection module is a target analyte detection device, characterized in that it includes a plurality of photodetectors, and each photodetector is configured to detect emission light emitted from each sample area. You can.

In one embodiment of the present invention, the excitation light filter unit includes an excitation light filter wheel in which a plurality of filters are arranged in a circumferential direction, and an excitation light filter wheel driving unit that rotates the excitation light filter wheel. It may be a target analyte detection device.

In one embodiment of the present invention, the light emitting module includes a plurality of light source elements, and at least two light source elements irradiate excitation light that passes through different filters among a plurality of filters arranged on the same excitation light filter wheel. It may be a target analyte detection device, characterized in that it is arranged to do so.

According to one embodiment of the present invention, the light emitting module includes a TIR lens, and the beam angle of the TIR lens is controlled to a specific range, so that the luminous intensity of the excitation light irradiated to the sample area without deteriorating the optical uniformity of each area of the sample area. By increasing, the signal-to-noise ratio (SNR) can be improved.

The effects of the present invention are not limited to the effects described above, and should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

Figure 1 is a side view of a target analyte detection device according to an embodiment.
Figure 2(a) shows the relative intensity of light measured according to the angle away from the center of the objective lens when light is irradiated from the light emitting module to the objective lens. Figure 2(b) is a conceptual diagram showing a case where the excitation light generated from the light emitting module passes through the objective lens and is irradiated to the sample area.
FIG. 3 is a diagram for explaining the positional relationship between a sample holder divided into a plurality of sample areas and a light-emitting module including a plurality of light source elements.
Figure 4 is a diagram for explaining the positional relationship between a sample holder divided into a plurality of sample areas and an excitation light filter unit.
Figure 5 is an exploded view of a lens housing including a lens, a spacer, and a field stop according to an embodiment of the present invention.
Figure 6 is a cross-sectional view of a lens housing including a lens, a spacer, and a field stop according to an embodiment of the present invention.
Figures 7 (a) to (c) each show cross-sectional views according to various embodiments of the field stop.

Hereinafter, the present invention will be described in detail through examples and illustrative drawings. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Additionally, when adding reference numerals to components in each drawing, it should be noted that the same components are given the same reference numerals as much as possible even if they are shown in different drawings. Additionally, in describing the present invention, if it is determined that a detailed description of a related known configuration or function may obscure the gist of the present invention, the detailed description will be omitted.

Additionally, in describing the components of the present invention, terms such as first, second, A, B, (a), (b), (i), (ii), etc. may be used. These terms are only used to distinguish the component from other components, and the nature, order, or order of the component is not limited by the term. When a component is described as being "connected," "coupled," or "connected" to another component, that component may be directly connected or connected to the other component, but there is another component between each component. can be understood as being able to be “connected,” “coupled,” or “connected.”

The present invention relates to a detection device for detecting target analytes in a sample.

As used herein, “sample” may include biological samples (e.g., cells, tissues, and fluids from biological sources) and non-biological samples (e.g., food, water, and soil). The biological samples include viruses, bacteria, tissues, cells, blood (e.g. whole blood, plasma and serum), lymph, bone marrow fluid, saliva, sputum, swabs, aspiration, milk and urine. , feces, ocular fluid, semen, brain extract, spinal fluid, joint fluid, thymic fluid, bronchial lavage fluid, ascites, and amniotic fluid. Additionally, the sample may include natural and synthetic nucleic acid molecules isolated from biological sources. According to one embodiment of the present invention, the sample may include additional substances such as water, deionized water, saline solution, pH buffer, acidic solution, and basic solution.

A target analyte refers to an analyte that is subject to analysis. The analysis may mean, for example, obtaining information about the presence, content, concentration, sequence, activity or characteristics of the analyte in the sample. Analytes can include a variety of substances (e.g., biological substances and non-biological substances such as compounds). Specifically, the analytes may include biological substances such as nucleic acid molecules (e.g., DNA and RNA), proteins, peptides, carbohydrates, lipids, amino acids, biological compounds, hormones, antibodies, antigens, metabolites, and cells. there is. According to one embodiment of the present invention, the analyte may be a nucleic acid molecule.

Therefore, the target analyte detection device of the present invention may be a target nucleic acid detection device. The target nucleic acid detection device allows the nucleic acid reaction in the sample to proceed and detects the target nucleic acid through this.

Nucleic acid reaction refers to a series of physical and chemical reactions that generate a signal depending on the presence or amount of nucleic acid of a specific sequence in a sample. The nucleic acid reaction may be a reaction including binding of a nucleic acid of a specific sequence in a sample to another nucleic acid or substance, or replication, cleavage, or decomposition of a nucleic acid of a specific sequence in the sample. The nucleic acid reaction may be a reaction involving a nucleic acid amplification reaction. The nucleic acid amplification reaction may include amplification of a target nucleic acid. The nucleic acid amplification reaction may be a reaction that specifically amplifies a target nucleic acid.

The nucleic acid reaction may be a signal-generating reaction, which is a reaction capable of generating a signal depending on the presence/absence or amount of the target nucleic acid in the sample. This signal-generating reaction may be a genetic analysis process such as PCR, real-time PCR, or microarray.

Various methods are known for generating an optical signal indicating the presence of a target nucleic acid using a nucleic acid reaction. Representative examples include: the TaqManTM probe method (U.S. Patent No. 5,210,015), the molecular beacon method (Tyagi et al., Nature Biotechnology v.14 MARCH 1996), the Scorpion method (Whitcombe et al., Nature Biotechnology 17:804- 807 (1999)), Sunrise or Amplifluor method (Nazarenko et al., 2516-2521 Nucleic Acids Research, 25(12):2516-2521 (1997), and US Patent No. 6,117,635), Lux method (US Patent No. 7,537,886), CPT (Duck P, et al. Biotechniques, 9:142-148 (1990)), LNA method (US Patent No. 6,977,295), Plexor method (Sherrill CB, et al., Journal of the American Chemical Society, 126:4550-4556 (2004)), HybeaconsTM (D. J. French, et al., Molecular and Cellular Probes (2001) 13, 363-374 and U.S. Patent No. 7,348,141), double-labeled self- Quenched probe (Dual-labeled, self-quenched probe; U.S. Patent No. 5,876,930), hybridization probe (Bernard PS, et al., Clin Chem 2000, 46, 147-148), PTOCE (PTO cleavage and extension) method ( WO 2012/096523), PCE-SH (PTO Cleavage and Extension-Dependent Signaling Oligonucleotide Hybridization) method (WO 2013/115442), PCE-NH (PTO Cleavage and Extension-Dependent Non-Hybridization) method (PCT/KR2013/012312) and CER method (WO 2011/037306).

The target analyte detection device according to an embodiment of the present invention may be a nucleic acid detection device and can detect a signal that occurs depending on the presence of a target nucleic acid. A nucleic acid detection device can detect a signal by amplifying it along with nucleic acid amplification. Alternatively, the nucleic acid detection device may detect the signal by amplifying the signal without amplifying the nucleic acid. Preferably, the signal is detected accompanied by nucleic acid amplification.

The target analyte detection device 10 according to an embodiment of the present invention may include a nucleic acid amplification device.

A nucleic acid amplification device refers to a device that can perform a nucleic acid amplification reaction to amplify a nucleic acid having a specific nucleotide sequence. Methods for amplifying the nucleic acids include the polymerase chain reaction (PCR) and ligase chain reaction (LCR) (U.S. Patents Nos. 4,683,195 and 4,683,202; PCR Protocols: A) Guide to Methods and Applications (Innis et al., eds, 1990), strand displacement amplification (SDA) (Walker, et al. Nucleic Acids Res. 20(7):1691-6 (1992); Walker PCR Methods Appl 3(1):1-6 (1993)), transcription-mediated amplification (Phyffer, et al., J. Clin. Microbiol. 34:834-841 (1996); Vuorinen, et al., J. Clin. Microbiol. 33:1856-1859 (1995)), nucleic acid sequence-based amplification (NASBA) (Compton, Nature 350(6313):91-2 (1991) ), rolling circle amplification (RCA) (Lisby, Mol. Biotechnol. 12(1):75-99 (1999); Hatch et al., Genet. Anal. 15(2):35-40 (1999) )) and Q-Beta Replicase (Lizardi et al., BiolTechnology 6:1197 (1988)).

The target analyte detection device 10 according to one embodiment may be a device that performs a nucleic acid amplification reaction while changing temperature. For example, to amplify DNA (deoxyribonucleic acid) with a specific base sequence, a nucleic acid amplification device can perform a denaturing step, an annealing step, and an extension (or amplification) step. there is.

The denaturation step is a step in which double-stranded DNA is separated into single-stranded DNA by heating a solution containing a sample containing double-stranded DNA, which is a template nucleic acid, and a reagent to a specific temperature, for example, about 95°C. The annealing step provides an oligonucleotide primer having a nucleotide sequence complementary to the nucleotide sequence of the nucleic acid to be amplified, cools the separated single-stranded DNA to a specific temperature, for example, 60°C, and amplifies the single-stranded DNA. This is the step of forming a partial DNA-primer complex by binding a primer to a specific nucleotide sequence. In the extension step, after the annealing step, the solution is maintained at a specific temperature, for example, 72°C, and a double-stranded DNA is formed based on the primer of the partial DNA-primer complex by DNA polymerase. do.

By repeating the three steps described above, for example, 10 to 50 times, DNA having the specific nucleotide sequence can be amplified exponentially. In some cases, the nucleic acid amplification device may perform the annealing step and the extension step simultaneously. In this case, the nucleic acid amplification device may complete the first cycle by performing two steps consisting of a denaturation step and an annealing/extension step.

In particular, the target analyte detection device 10 according to one embodiment is a device that performs a nucleic acid amplification reaction and a reaction that generates an optical signal depending on the presence of the nucleic acid while accompanied by a change in temperature and detects the generated optical signal. You can.

Figure 1 is a side view of a target analyte detection device according to an embodiment.

The target analyte detection device 10 according to one embodiment includes a sample holder 100, a light emitting module 200, and a detection module 300.

A sample refers to a substance for which the target analyte is to be detected. The samples include biological samples (e.g., cells, tissues, and fluids from biological sources) and non-biological samples (e.g., food, water, and soil). Biological samples include viruses, bacteria, tissues, cells, blood, serum, plasma, lymph, sputum, swab, aspiration, bronchoalveolar lavage fluid, milk, urine, feces, ocular fluid, saliva, Including, but not limited to, semen, brain extract, spinal fluid (SCF), appendix, spleen and tonsil tissue extract, amniotic fluid and ascites. Additionally, the sample may include natural and synthetic nucleic acid molecules isolated from biological sources.

In the present invention, the sample may contain substances necessary for detecting the target analyte. For example, the sample may include additional substances such as water, deionized water, saline solution, pH buffer, acidic solution, basic solution. Additionally, according to one embodiment of the present invention, the sample may include an optical label. The optical label refers to a label that generates an optical signal depending on the presence of a target nucleic acid. The optical label may be a fluorescent label. The fluorescent label useful in the present invention may include any molecule known in the art.

The light emitting module 200 according to the present invention supplies appropriate optical stimulation to the sample accommodated in the sample holder 100, and the detection module 300 detects an optical signal generated from the sample in response to this.

The optical signal may be luminescence, phosphorescence, chemiluminescence, fluorescence, polarized fluorescence, or other colored signal. The optical signal may be an optical signal generated in response to optical stimulation applied to the sample.

The sample holder 100 is formed with a sample receiving portion that accommodates the sample. That is, the sample holder 100 is a component that directly accommodates the sample in the sample receiving unit or accommodates a reaction vessel containing the sample.

As used herein, the expression “sample holder 100 is capable of receiving a sample” generically refers to cases where the sample holder 100 receives a sample directly in a sample receiving portion or receives a reaction vessel containing a sample. can be used for

The sample holder 100 positions the sample at a predetermined position so that optical stimulation from the light emitting module 200 reaches the sample and an optical signal generated from the sample reaches the detection module 300.

Heat may be supplied to the sample holder 100 by a heat generating element, and the heat is transferred to the sample accommodated directly in the sample holder 100 or the sample accommodated in the reaction vessel.

Reaction vessels can be made of a variety of materials, such as plastic, ceramic, glass, or metal.

The sample holder 100 that accommodates the reaction vessel may have the shape of a block or plate. The sample holder 100 accommodating the reaction vessel may include a recess accommodating the reaction vessel, for example a well, or may have a flat surface. The sample holder 100 that accommodates the reaction vessel may have a structure that guides the position of the reaction vessel or fixes the sample reaction vessel.

One sample holder 100 is configured to accommodate one or more samples.

A representative example of a sample holder 100 that accommodates a reaction vessel is a thermal block. The thermal block includes a plurality of wells or holes, in which reaction vessels may be accommodated.

That the sample holder 100 accommodates the sample reaction vessels may mean that the sample reaction vessels are placed in a plurality of wells formed in the sample holder 100 or in an assigned position on the sample holder.

The reaction vessel is used to accommodate the sample to be analyzed, and may be of various types, such as a tube, a vial, a strip connected to a plurality of single tubes, or a plate connected to a plurality of tubes. Includes a plate, microcard, chip, cuvette, or cartridge.

The sample holder 100 that directly accommodates the sample may have the shape of the reaction vessel described above and may be formed of the material of the reaction vessel described above.

In one embodiment, the sample holder 100 may be formed of a thermally conductive material. When the sample holder 100 is in direct contact with a sample or with reaction vessels, heat may be transferred from the sample holder 100 to the sample or the sample in the reaction vessel.

The sample holder 100 may be made of metal such as aluminum, gold, silver, nickel, or copper, or may be made of plastic or ceramic.

In this way, the sample holder 100 is formed to accommodate a plurality of samples, and the temperature of the plurality of samples is adjusted to enable a reaction for detection, such as a nucleic acid amplification reaction, to occur. As an example, when the sample holder 100 is a thermal block in which a plurality of wells are formed, the sample holder 100 is formed as one thermal block, and all wells of the thermal block are formed not to be thermally independent from each other. You can. In this case, the temperatures of all wells in which samples are received in the sample holder 100 are the same, and the temperatures of the received samples cannot be adjusted according to different protocols.

As another example, the sample holder 100 may be configured to control the temperature of some of the samples accommodated in the sample holder 100 according to different protocols. In other words, the sample holder 100 may include two or more thermally independent reaction regions. Each reaction zone is thermally independent. Heat does not transfer from one reaction zone to another. For example, there may be an insulating material or an air gap between the reaction regions. The temperature of each reaction zone can be controlled independently. A reaction protocol including temperature and time can be individually set for each of the reaction zones, and each of the reaction zones can perform the reaction according to an independent protocol. Since the reaction proceeds according to an independent protocol in the reaction regions, the time points of light detection in the reaction regions are independent of each other.

According to one embodiment, the sample holder 100 may be divided into a plurality of sample areas 110. The sample area 110 is an area divided by the excitation light irradiation area of the light emitting module 200.

That is, since the light emitting module 200 of the present disclosure may include a plurality of light source elements 210, the sample holder 100 may be divided into a plurality of sample areas. Each of the plurality of sample areas 110 refers to an area on the sample holder 100 where samples in which an optical signal detection reaction is performed by the same light element 210 are located. In other words, the sample area 110 of the present disclosure refers to a group of reaction sites where an optical signal detection reaction is performed by the same light element 210 among a plurality of reaction sites included in the sample holder 100. That is, the sample area 110 is an area divided by the excitation light irradiation area of the light element 210. One or more wells or holes may be formed in each sample area 110. Figure 1 shows an example in which the sample holder 100 is divided into two sample areas 110a and 110b, but the sample holder 100 of the present invention is not limited thereto. The sample holder 100 of the present invention includes, for example, 2, 3, 4, 5, 6, 8, 10, 12, 16, 20, and 24 sample areas. It may be a sample holder 100.

According to one embodiment, when the sample holder 100 is a thermal block, empty spaces may exist between wells to reduce thermal capacity.

According to one embodiment, a plurality of wells or a plurality of holes in the sample holder 100 are formed in a regular arrangement. For example, a plurality of wells are formed in a matrix form consisting of columns and rows. 16-well in 4 x 4 format, 24-well in 6 x 4 format, 32-well in 4 x 8 format, 60-well in 5 x 12 format, 90-well in 5 x 18 format, 8 x 12 well in format. It can be formed in various shapes such as 96-well, and is not limited to these, but 16-well, 32-well, and 96-well can be mainly used. The shape, size, etc. of the wells may be determined to suit the receiving reaction vessel.

According to one embodiment, the target analyte detection device 10 may include a thermal module 410, a thermal lid 420, a heat dissipation plate 430, and a cooling fan 440.

The thermal module 410 can increase or decrease the temperature of the sample holder 100. The thermal module 410 is disposed below the sample holder 100 and may contact the sample holder 100 to transfer heat to the sample holder 100 or absorb heat from the sample holder 100. As an example, the thermal module 410 may be a Peltier element or a heat wire, and may include an FPCB board to which they are connected.

The thermal module 410 may have various shapes depending on the embodiment of the sample holder 100 or the method of supplying electricity. For example, it may be a polygonal plate shape with an area sufficient to cover a specific area of the sample holder 100, or a heat wire shape in which electrically conductive terminals are pressed together in a linear form. These thermal modules 410 may be It is electrically connected to the power module and can generate heat using the power provided from the power module.

The thermal lid 420 provides one or more of heat and pressure to the reaction vessels accommodated in the sample holder 100. The thermal lid 420 may contact the covers of the reaction vessels and provide pressure to the reaction vessels by pressing the covers of the reaction vessels. Additionally, the thermal lid 420 can maintain a high temperature. For example, the thermal lid 420 may include a heat plate (not shown) that maintains a temperature of 105°C.

The thermal lid 420 includes a plurality of holes. The holes of the thermal lid 420 are formed at positions corresponding to the wells of the sample holder 100. Excitation light and emission light may pass through the hole of the thermal lid 420.

The heat dissipation plate 430 may be located below the sample holder 100 or the thermal module 410. The heat dissipation plate 430 is a passive heat exchanger and is disposed below the sample holder 100 or thermal module 410 to efficiently radiate heat from the sample holder 100 or thermal module 410 to the outside. do.

The heat dissipation plate 430 may be formed of metal, ceramic, or plastic. The heat dissipation plate 430 may include a plurality of heat dissipation fins to expand the heat dissipation area. A plurality of heat dissipation fins may be arranged vertically in a row or radially on the base portion of the heat dissipation plate 430. Heat dissipation fins formed on the heat dissipation plate 430 may be arranged in various directions depending on the embodiment. For example, straight fins may be arranged in the X-axis or Y-axis direction of the base portion of the heat dissipation plate 130.

The cooling fan 440 provides external air to cool the heat dissipation plate 430. The cooling fan 440 may cool the sample holder 100 by cooling the heat dissipation plate 430 thermally connected to the sample holder 100 rather than directly cooling the sample holder 100. The cooling fan 440 may use various types of known cooling fans. For example, axial fans, centrifugal fans, and cross flow fans can be used.

Meanwhile, the target analyte detection device 10 of the present invention includes a light emitting module 200 and an objective lens 600. The light emitting module 200 includes a light element 210, a TIR lens 220, and an excitation light filter unit 230.

The light emitting module 200 emits light to excite the optical label included in the sample. Light emitted by the light element 210 of the light emitting module 200 may be displayed as excitation light. The light emitted by the sample can be expressed as emission light. The path of the excitation light emitted from each light element 210 may be expressed as an excitation path. The path of the emission light emitted from the sample can be expressed as an emission path. The light source element 210 and the photodetector 310 may be placed at fixed positions to maintain an accurate optical path with respect to the sample holder 100.

The light source device 210 may be a laser unit including a light emitting diode (LED) including an organic LED, an inorganic LED, and a quantum dot LED, a tunable laser, a He-Ne laser, and an Ar laser. According to one embodiment of the present invention, the light source device 210 may be an LED.

According to one embodiment, the light emitting module 200 may further include a light source element support 240 for fixing the light source element 210. The light source element support 240 arranges and fixes the light source elements 210 so that the light source elements 210 can evenly supply light to the target sample area. The light element support 240 may be a board on which wiring is formed so that a voltage can be applied from the outside, that is, a printed circuit board (PCB).

According to one embodiment, when the light emitting module 200 includes a plurality of light source elements 210, each of the plurality of light source elements 210 irradiates light to a different sample area 110 of the sample holder 100. is formed That is, each light element 210 radiates excitation light to the individually assigned sample area 110. At this time, the detection module 300 may include a plurality of photodetectors 310, and each of the plurality of photodetectors 310 is assigned a sample area 110 among the different sample areas 110 of the sample holder 100. ) is formed to detect the emission light emitted from the. Each photodetector 310 can detect an optical signal occurring in the assigned sample area 110.

The TIR (Total Internal Reflection) lens 220 is disposed or combined adjacent to the light source element 210 and guides the excitation light emitted from the light source element 210. The TIR lens 220 is formed to guide the excitation light through total internal reflection in order to increase the luminous intensity (LI) of the excitation light emitted from the light source element 210, that is, the intensity of the excitation light. That is, the TIR lens 220 performs a function of collecting light emitted broadly from the light element 210. If the intensity of the excitation light irradiated from the light source element 210 is increased using the TIR lens 220, the signal to noise ratio (SNR) in the photodetector 310 increases, thereby increasing the accuracy of target analyte detection. You can. Meanwhile, a collimating lens that collimates the excitation light emitted from the light source element 210, that is, turns it into parallel light, may be used instead of the TIR lens 220.

When the light emitting module 200 includes a plurality of light elements 210 and a plurality of TIR lenses 220, each TIR lens 220 may be disposed or coupled to each light element element 210 adjacent to each other. According to one embodiment, the light source element 210 and the TIR lens 220 may be formed as one assembly, and the assembly includes a light element support 240 to evenly supply light to the target sample area. Can be arranged and fixed.

The objective lens 600 is disposed along the optical axis at the top of the sample holder 100 and guides or condenses the excitation light emitted from the light emitting module 200 in parallel toward the sample receiving portion of the sample holder 100. is formed The objective lens 600 may be disposed at least one along the optical axis, and preferably, two objective lenses 600 may be disposed along the optical axis.

Meanwhile, when the sample holder 100 is divided into a plurality of sample areas 110, each objective lens 600 may be placed on top of each sample area 110. At this time, the objective lens 600 may be formed to have a planar shape corresponding to the sample area 110.

Figure 2(a) shows the relative intensity of light measured according to the angle away from the center of the objective lens when light is irradiated from the light emitting module to the objective lens, and Figure 2(b) shows the excitation light generated from the light emitting module. This is a conceptual diagram showing the case where irradiation passes through the objective lens and into the sample area.

Referring to FIG. 2, the light element 210, the TIR lens 220, the objective lens 600, and the sample receiving portion of each sample area 110 may be arranged along the same optical axis, that is, in the vertical direction. Excitation light generated from the light source element 210 may sequentially pass through the TIR lens 220 and the objective lens 600 and be irradiated to the sample receiving portion of the sample area 110.

According to one embodiment, the sample area 110 may be formed to have a 16-sample receiving portion in a 4 x 4 shape. At this time, the sample area 110 may be divided into A ZONE, B ZONE, and C ZONE depending on the area where the excitation light reaches. A ZONE is a zone containing 4 sample accommodating units in the center, B ZONE is a zone containing 6 sample accommodating units on the sides, and C ZONE is a zone containing 4 sample accommodating units in the corners.

Referring to FIG. 2, the TIR lens 220 controls and guides the light widely irradiated from the light source element 210 to the sample area 110 to form a constant beam angle (θ _a ).

_b)이란, 대물렌즈(600)의 최외곽과 광원소자(210)가 이루는 각도의 하프각(half angle)을 의미한다. 도 1에서, 샘플 홀더(100)를 향하여 개구되는 차단모듈(500)의 개구부(512)가 대물렌즈(600) 보다 작게 도시되었으나, 실질적으로 개구부(512)는 대물렌즈의 크기와 대응되는 크기를 갖도록 형성될 수 있다. At this time, the beam angle (θ _a ) of the TIR lens is when the light emitted from the light element 210 passes through the TIR lens 220 and reaches the area corresponding to the distance (L) to the objective lens 600. It refers to the half angle of the angle formed between the light source element 210 and a point having a luminous intensity corresponding to 50% of the relative luminous intensity measured at the center (O) of the area. And, the angle between the light element and the objective lens (

_b ) means the half angle of the angle formed between the outermost edge of the objective lens 600 and the light source element 210. In FIG. 1, the opening 512 of the blocking module 500 that opens toward the sample holder 100 is shown to be smaller than the objective lens 600, but in reality, the opening 512 has a size corresponding to the size of the objective lens. It can be formed to have.

According to one embodiment, the beam angle (θ _a ) of the TIR lens may be 16° to 24°, and preferably 18° to 22°. And, the angle (θ _b ) between the light element and the objective lens may be 10° to 15°.

According to one embodiment, the beam angle (θ _a ) of the TIR lens and the angle (θ _b ) between the light element and the objective lens may satisfy the following equation.

Formula: 0.55 ≤ θ _b /θ _a ≤ 0.75

When θ _b /θ _a is greater than 0.75, the luminous intensity (LI) of the excitation light irradiated to the sample area 110 increases and the signal-to-noise ratio (SNR) in the photodetector 310 is improved, thereby improving the accuracy of detecting the target analyte. However, the relative luminance difference between the A zone and the C zone of the sample area 110 may increase, thereby lowering the optical uniformity for each zone.

If θ _b /θ _a is less than 0.55, the relative luminance difference between the A ZONE and C ZONE of the sample area 110 becomes smaller and the optical uniformity for each zone can be increased, but in the sample area 110 Since the luminous intensity (LI) of the irradiated excitation light is not sufficient, the signal-to-noise ratio (SNR) in the photodetector 310 may not be improved.

The excitation light filter unit 230 filters the light emitted from the light source element 210. The filter means that among the light emitted from the light source element 210, selectively passes light in a specific wavelength band or does not selectively pass light in a specific wavelength band. The excitation light filter unit 230 selectively passes light in a specific wavelength band among the light emitted from the light source element 210 to be irradiated to the sample. As a result, only specific optical labels among the optical labels included in the sample generate optical signals.

The excitation light filter unit 230 is spaced apart from the TIR lens 220 and is formed to allow the excitation light guided by the TIR lens 220 to pass through.

The excitation light filter unit 230 includes an excitation light filter wheel 232 in which a plurality of filters 231 having different wavelength bands are arranged in the circumferential direction, and an excitation light filter wheel driver that rotates the excitation light filter wheel 232. It may include (234).

Filter 231 may be a bandpass filter. The bandpass filter refers to a filter that selectively transmits light in a certain wavelength band. The wavelength band of light passing through the bandpass filter is called the passband of the filter 231. The passband may be displayed in the form of a wavelength band. The filter 231 including a specific passband refers to a filter that passes light of a wavelength included in the specific passband.

Each of the plurality of filters 231 disposed on the excitation light filter wheel 232 can pass light that can excite different optical labels. Therefore, according to one embodiment, the passbands of each of the plurality of filters 231 may not overlap or may be different from each other. Each of the plurality of filters 231 may be arranged to selectively excite different optical labels.

The excitation light filter wheel driving unit 234 is connected to the rotation shaft 233 of the excitation light filter wheel 232 and rotates the excitation light filter wheel 232. The excitation light filter wheel driving unit 234 may include, for example, a motor. The motor may be, for example, an AC motor, DC motor, step motor, servo motor, or linear motor, and is preferably a step motor. Each filter 231 of the excitation light filter wheel 232 may be alternately arranged on the light source element 210 by the excitation light filter wheel driving unit 234.

According to one embodiment, when the light emitting module 200 includes a plurality of light source elements 210, at least two light source elements 210 are connected to a plurality of filters 231 disposed on the same excitation light filter wheel 232. It may be arranged to irradiate excitation light that passes through different filters.

FIG. 3 is a diagram for explaining the positional relationship between a sample holder divided into a plurality of sample areas and a light-emitting module having a plurality of light element elements, and FIG. 4 is a diagram showing the position of the sample holder divided into a plurality of sample areas and the excitation light filter unit. This is a drawing to explain the relationship.

Referring to Figures 3 and 4, the sample holder 100 according to one embodiment includes a 96-sample receiving portion in the form of 8 can be distinguished. The sample areas 110a, 110b, 110c, 110d, 110e, and 110f may each include a 16-sample receiving portion in a 4 x 4 shape.

The light emitting module 200 according to one embodiment may include six light element elements 210 fixed to the light element support 240. A TIR lens 220 may be disposed or combined adjacent to each light element 210. The six light elements 210 are formed to irradiate light to different sample areas 110a, 110b, 110c, 110d, 110e, and 110f of the sample holder 100, respectively. That is, each light element 210 irradiates excitation light to individually assigned sample areas 110a, 110b, 110c, 110d, 110e, and 110f.

Six light source elements 210 can be irradiated to six sample areas 110a, 110b, 110c, 110d, 110e, and 110f through two excitation light filter units 230. That is, the four light source elements 210 pass through different filters 231 among the plurality of filters 231 formed in one excitation light filter unit 230 to produce four sample areas 110a, 110b, 110c, and 110d. can be irradiated, and the two light element elements 210 pass through different filters 231 among the plurality of filters 231 formed in the other excitation light filter unit 230 to produce two sample areas 110e and 110f. ) can be investigated.

Meanwhile, in FIGS. 3 and 4, one light source element 210 is shown as corresponding to one sample area 110, but differently, a plurality of light element elements may correspond to one sample area.

In addition, Figures 3 and 4 show that four light source elements 210 share one excitation light filter wheel 232, and four filters 231 are provided on the excitation light filter wheel 232. However, unlike this, it is also possible for a plurality of light source elements less than four to share one excitation light filter wheel, or for a plurality of light source elements of five or more to share one excitation light filter wheel.

In addition, it is possible for one excitation light filter wheel to be provided with a plurality of filters less than four, or a plurality of filters with five or more filters. For example, four light elements may be arranged to share one excitation light filter wheel having five filters. Each of the five filters is disposed on the same radius around the rotation axis of the excitation light filter wheel, and may be provided at any one of eight positions divided at equal intervals in the circumferential direction.

Meanwhile, the description of the light source element 210 and the excitation light filter wheel 230 described above can be applied to the photodetector 310 and the emission light filter wheel 320. And the corresponding structure of the light source element 210 and the excitation light filter wheel 230 may be the same as or different from the corresponding structure of the photodetector 310 and the emission light filter wheel 320. Additionally, the number and arrangement of filters of the excitation light filter wheel 230 may be the same as or different from the number and arrangement of filters of the emission light filter wheel 320.

Meanwhile, according to Figure 1, the target analyte detection device 10 of the present invention includes a detection module 300. The detection module 300 detects an optical signal generated from a sample accommodated in the sample holder 100. The detection module 300 includes a photodetector 310, an emission light filter unit 320, a plurality of lenses 330, a field stop 340, and a spacer 350.

The photodetector 310 may detect an optical signal by generating an electric signal according to the intensity of the optical signal. The photodetector 310 is configured to detect light emitted from an optical label included in the sample. The photodetector 310 may detect the amount of light for each wavelength by distinguishing the wavelengths of light, or may detect the total amount of light regardless of the wavelength. Specifically, the photodetector 310 may use, for example, a photo diode, a photo diode array, a photo multiplier tube (PMT), a CCD image sensor, a CMOS image sensor, or an avalanche photodiode (APD).

The emission light filter unit 320 is spaced apart from the photodetector 310 and is formed to allow emission light emitted from the sample to pass through.

The emission light filter unit 320 includes an emission light filter wheel 322 in which a plurality of filters 321 having different wavelength bands are arranged in the circumferential direction, and an emission light filter wheel driver that rotates the emission light filter wheel 322. It may include (324).

The plurality of filters 321 disposed on the emission light filter wheel 322 are filters for selectively passing emission light emitted from the optical label included in the sample. If light in a different wavelength band other than the emission light emitted from the optical label included in the sample is detected by the photodetector 310, the optical signal cannot be accurately detected. The filter 321 allows the target analyte to be accurately detected by selectively passing the emission light emitted from the optical label.

The emission light filter wheel driving unit 324 is connected to the rotation shaft 323 of the emission light filter wheel 322 and rotates the emission light filter wheel 322. The emission light filter wheel driving unit 324 may include, for example, a motor. The motor may be, for example, an AC motor, DC motor, step motor, servo motor, or linear motor, and is preferably a step motor. Each filter 321 of the emission light filter wheel 322 may be alternately arranged in the photodetector 310 by the emission light filter wheel driving unit 324.

According to one embodiment, the detection module 300 includes a plurality of photodetectors 310, and at least two photodetectors 310 are selected from among the plurality of filters 321 disposed on the same emission light filter wheel 322. It may be arranged to detect emitted light that has passed through different filters.

The plurality of lenses 330 are arranged along the optical axis of the emitted light entering the photodetector 310, and adjust the focus so that the emitted light is focused on the photodetector 310. That is, the emission light emitted from the sample may sequentially pass through the plurality of lenses 330 and the emission light filter unit 320 and form an image on the photodetector 310. The plurality of lenses 330 may be configured in various numbers, such as 2, 3, 4, or 5, depending on the degree of focus control of the emitted light. Additionally, the plurality of lenses 330 may be composed of a combination including one or more concave lenses and one or more convex lenses.

The spacer 350 is disposed between each lens of the plurality of lenses 330 to maintain a constant distance between each lens. As an example, the spacer 350 may be a gap adjustment ring. A plurality of spacers 350 may be arranged corresponding to the number of lenses 330.

The field stop 340 is disposed at a specific position between the plurality of lenses 330 to block light other than the emitted light from being focused on the photodetector 310. For example, the field stop 340 may be a field stop, and the inner diameter D of the field stop 340 may be smaller than the inner diameter of the spacer 350. That is, the field stop 340 can reduce optical noise by preventing light reflected from, for example, the thermal lid 420, rather than the emitted light emitted from the sample, from reaching the photodetector 310. Meanwhile, the field stop 340 can also perform the function of maintaining a constant distance between lenses. That is, the field stop 340 can perform one or more of the functions of blocking light other than the emitted light and maintaining the distance between the lenses constant.

Figure 5 is an exploded view of a lens housing including a lens, a spacer, and a field stop according to an embodiment of the present invention, and Figure 6 is a cross-sectional view of a lens housing including a lens, a spacer, and a field stop according to an embodiment of the present invention. am.

5 and 6, the detection module 300 of the present invention according to one embodiment includes a first lens housing 361 and a second lens housing 362 that are axially coupled to each other and each accommodate one or more lenses. ) includes. At this time, the first lens housing 361 may be disposed on the distal side from the photodetector 310, and the second lens housing 362 may be disposed on the proximal side from the photodetector 310. The first lens housing 361 and the second lens housing 362 may be coupled using various coupling methods to facilitate coupling with each other, but are preferably coupled using a screw coupling method.

According to one embodiment, the field stop 340 includes a lens disposed proximally from the photodetector 310 inside the first lens housing 361 and a photodetector ( 310) may be located between the lenses disposed on the distal side.

More specifically, the first lens housing 361 sequentially accommodates the first lens 331, the first spacer 351, the second lens 332, and the fieldstop 340 from the distal side from the photodetector 310. can do. At this time, the field stop 340 is inserted and coupled into the first lens housing 361 and presses the first lens 331 and the second lens 332 inside the first housing 361 to a predetermined position. so that it can be fixed to . As an example, the coupling between the field stop 340 and the first lens housing 361 may be a screw coupling, and when the field stop 340 is screw coupled with the first lens housing 361, the torque is measured to form the first lens housing 361. It can be determined whether the lens 331 and the second lens 332 are at a predetermined position.

The second lens housing 362 includes a third lens 333, a second spacer 352, a fourth lens 334, a third spacer 353, and a fifth lens ( 335) and a fourth spacer 354 can be accommodated. At this time, the fourth spacer 354 is inserted and coupled into the second lens housing 362 and the third lens 333, fourth lens 334, and fifth lens inside the second housing 362. Pressurize (335) so that it can be fixed in the designated position. As an example, the fourth spacer 354 and the second lens housing 362 may be coupled by screwing, and when the fourth spacer 354 is screwed with the second lens housing 362, the torque is measured and the fourth spacer 354 is screwed together with the second lens housing 362. It can be determined whether the third lens 333, fourth lens 334, and fifth lens 335 are at designated positions.

As such, the detection module 300 includes a first lens housing 361 and a second lens housing 362 each accommodating one or more lenses, and the first lens housing 361 and the second lens housing 362 Since the field stop 340 is provided at the coupling area, it is easy to combine and fix each lens in position, and maintenance can be easy.

Figures 7 (a) to (c) each show cross-sectional views according to various embodiments of the field stop. According to FIG. 7, the second lens 332 may be a convex lens in the direction of the photodetector 310, and the field stop 340 may be formed to have a step on one side supporting the convex side of the second lens 332. (Figure 7(a)), may be formed inclinedly (Figure 7(b)), or may be formed with a curved surface corresponding to the curved surface of the second lens (Figure 7(c)). That is, by expanding the contact area between the convex side of the second lens 332 and the field stop 340, the pressure applied to the second lens 332 is distributed to prevent the second lens 332 from being damaged. You can.

On the other hand, according to FIG. 1, the target analyte detection device 10 of the present invention includes a blocking module 500.

The blocking module 500 includes a beam splitter 520 and a blocking unit 510 disposed along the path of excitation light and emission light formed by the beam splitter 520.

The beam splitter 520 reflects and transmits excitation light incident from the light emitting module 200 and reflects and transmits light emitted from the sample. Accordingly, the excitation light from the light emitting module 200 reaches the sample accommodated in the sample holder 100 and the emission light from the sample reaches the detection module 300.

The blocking unit 510 may be arranged according to the path of excitation light and the path of emission light. The blocking portion 510 forms an internal passage determined by the path of the excitation light. The internal passage can be determined by the path of excitation light and the path of emission light.

The blocking unit 510 allows the excitation light irradiated to the sample from the light emitting module 200 and the emission light emitted to the detection module 300 to pass through the internal passage of the blocking unit 510. At this time, in some sections of each internal passage, excitation light and emission light may pass through the same path. Since the emission light is emitted only when the excitation light is irradiated, the two paths of the excitation light and the emission light do not overlap, but can pass through the same path. When light is irradiated to the sample holder 100, emission light is emitted from the sample of the irradiated sample holder 100 in response to this until the emission light reaches the beam splitter 520 accommodated in the blocking unit 510. The path of the emission light and the path until the excitation light is irradiated to the sample may be the same.

The path of the excitation light includes a first optical path from the beam splitter 520 to the sample holder 100 and a second optical path from the beam splitter 520 to the blocking unit 510. That is, the first optical path is the optical path through which the excitation light reaches the sample holder 100 from the beam splitter 520, and the second optical path is the optical path through which the excitation light reaches the blocking unit 510 from the beam splitter 520. It is a light path. After the excitation light reaches the beam splitter 520 from the light emitting module 200, it is divided into a first optical path and a second optical path. According to one embodiment, the light in the first optical path may be excitation light that passes through the beam splitter 520, and the light in the second optical path may be excitation light that is reflected in the beam splitter 520.

The blocking portion 510 includes an opening 511 opening toward the light emitting module 200, an opening 512 opening toward the sample holder 100, and an opening 513 opening toward the detection module 300. , Excitation light is irradiated from the light emitting module 200 through the openings 511, 512, and 513 and the internal passage to reach the sample holder 100, and the emission light is emitted from the sample holder 100 to reach the detection module 300. reaches.

The blocking module 500 has a noise-reducing structure 530 that adjusts the reflection angle of the reflected light to reduce the reflected light of the second optical path light by the blocking unit 510 from reaching the detection module 300. Includes.

The light of the second optical path reaching the blocking unit 510 may be absorbed and blocked by the blocking unit 510, and the reflected light that is not absorbed by the blocking unit 510 and is reflected by the noise reduction structure 530 is detected. Reaching the module 300 may be reduced and noise performance may be improved. The noise reduction structure 530 reduces the reflected light reflected from the blocking unit 510 from passing through the beam splitter 520 or being reflected from the beam splitter 520 to reach the detection module 300. Accordingly, the noise reduction structure 530 may be positioned opposite the beam splitter 520. When the noise reduction structure 530 is not included, the light of the second optical path reflected from the inner surface of the blocking unit 510 passes through the beam splitter 520 or is reflected by the beam splitter 520 and is transmitted to the detection module ( 300) can be reached. According to one embodiment, the noise reduction structure 530 may be located on the opposite side of the opening 513 facing the detection module 300 with the beam splitter 520 in between.

According to one embodiment, the noise reduction structure 530 may be formed to be inclined on the inner surface of the blocking portion 510. Since the noise reduction structure 530 is formed at an angle, the light of the second optical path reaching the blocking unit 510 is reflected at an angle with respect to the incident direction. That is, the noise reduction structure 530 can adjust the reflection angle of the reflected light depending on the degree of inclination.

The blocking module 500 includes a plurality of blocking modules arranged in each sample area 110 according to the path of the excitation light irradiated to each of the plurality of sample areas 110 and the path of the emission light emitted from each of the plurality of sample areas 110. It may include unit 510.

The blocking module 500 is configured so that when each of the plurality of light source elements 210 irradiates light to different sample areas 110, the excitation light is mixed between neighboring sample areas and a cross-talk phenomenon occurs. It is formed to be positioned based on the excitation light path for each different sample area 110 to prevent this from occurring.

The noise reduction structure 530 is formed in each of the plurality of blocking units 510, and can adjust the reflection angle of the reflected light to reduce the reflected light of the second optical path light included in each excitation light path from reaching the detection module 300. there is.

The above description is merely an illustrative explanation of the technical idea of the present invention, and those skilled in the art will be able to make various modifications and variations without departing from the essential quality of the present invention. Accordingly, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but are for illustrative purposes, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention shall be interpreted in accordance with the claims below, and all technical ideas within the scope equivalent thereto shall be construed as being included in the scope of rights of the present invention.

10: Target analyte detection device 100: Sample holder
110: sample area 200: light emitting module
210: light source element 220: TIR lens
230: excitation light filter unit 231: filter
232: excitation light filter wheel 233: rotation axis
234: Excitation light filter wheel driving unit 300: Detection module
310: photodetector 320: emission light filter unit
321: Filter 322: Emitted light filter wheel
323: Rotating shaft 324: Emitted light filter wheel driving unit
330: Lens 340: Fieldstop
350: spacer 361: first lens housing
362: Second lens housing 410: Thermal module
420: Thermal lid 430: Heat dissipation plate
440: Cooling fan 500: Blocking module
510: Blocking unit 520: Beam splitter
530: Noise reduction structure 600: Objective lens

Claims (13)

a sample holder configured to accommodate a sample;
a light emitting module configured to irradiate excitation light to a sample accommodated in the sample holder; and
It includes a detection module configured to detect emission light emitted from the sample,
The light emitting module is a target analyte detection device including a light source element that generates excitation light, and a TIR (Total Internal Reflection) lens that guides the excitation light. According to paragraph 1,
The light emitting module is disposed spaced apart from the TIR lens and includes an excitation light filter unit formed to allow excitation light guided by the TIR lens to pass. According to paragraph 1,
The TIR lens is a target analyte detection device, characterized in that it is formed to have a beam angle (θ _a ) of 16° to 24°. According to paragraph 3,
The TIR lens is a target analyte detection device, characterized in that it is formed to have a beam angle (θ _a ) of 18° to 22°. According to paragraph 1,
A target analyte detection device, characterized in that it includes one or more objective lenses disposed along the optical axis at the top of the sample holder to guide the excitation light in parallel. According to clause 5,
A target analyte detection device, characterized in that two objective lenses are arranged along the optical axis. According to clause 5,
The target analyte detection device, characterized in that the angle (θ _b ) between the light source element and the objective lens is 10° to 15°. According to clause 5,
The light source element, the TIR lens, the objective lens, and the sample receiving portion of the sample holder are arranged along the same optical axis,
Excitation light generated from the light source element sequentially passes through the TIR lens and the objective lens and is irradiated to the sample receiving portion of the sample holder. According to clause 5,
A target analyte detection device, characterized in that the beam angle (θ _a ) of the TIR lens and the angle (θ _b ) between the light element element and the objective lens satisfy the following equation.
Formula: 0.55 ≤ θ _b /θ _a ≤ 0.75 According to paragraph 1,
The sample holder is divided into a plurality of sample regions, and a plurality of sample receiving portions are formed in each region,
The light emitting module includes a plurality of light element elements, and each light element element is formed to irradiate excitation light to each sample area. According to clause 10,
The detection module includes a plurality of photodetectors, and each photodetector is configured to detect emission light emitted from each sample area. According to paragraph 2,
The excitation light filter unit includes an excitation light filter wheel in which a plurality of filters are arranged in a circumferential direction, and an excitation light filter wheel driving unit that rotates the excitation light filter wheel. According to clause 12,
The light emitting module includes a plurality of light element elements, and at least two light element elements are arranged to irradiate excitation light that passes through different filters among a plurality of filters arranged on the same excitation light filter wheel. Analyte detection device.