KR20230147224A - Method for producing high-purity chondroitin sulfate material using skate cartilage - Google Patents
Method for producing high-purity chondroitin sulfate material using skate cartilage Download PDFInfo
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- KR20230147224A KR20230147224A KR1020220045495A KR20220045495A KR20230147224A KR 20230147224 A KR20230147224 A KR 20230147224A KR 1020220045495 A KR1020220045495 A KR 1020220045495A KR 20220045495 A KR20220045495 A KR 20220045495A KR 20230147224 A KR20230147224 A KR 20230147224A
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- skate
- chondroitin sulfate
- cartilage
- skate cartilage
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- 210000000845 cartilage Anatomy 0.000 title claims abstract description 101
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 title claims abstract description 87
- 229920001287 Chondroitin sulfate Polymers 0.000 title claims abstract description 87
- 229940059329 chondroitin sulfate Drugs 0.000 title claims abstract description 87
- 239000000463 material Substances 0.000 title claims abstract description 41
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 33
- 239000012141 concentrate Substances 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 9
- 238000012545 processing Methods 0.000 claims abstract description 8
- 230000007065 protein hydrolysis Effects 0.000 claims abstract description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 28
- 102000035195 Peptidases Human genes 0.000 claims description 26
- 108091005804 Peptidases Proteins 0.000 claims description 26
- 239000000203 mixture Substances 0.000 claims description 22
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 18
- 102000005158 Subtilisins Human genes 0.000 claims description 16
- 108010056079 Subtilisins Proteins 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000011085 pressure filtration Methods 0.000 claims description 7
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 claims description 6
- 235000019465 surimi Nutrition 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 241000283690 Bos taurus Species 0.000 description 3
- 241000251730 Chondrichthyes Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 102000002704 Leucyl aminopeptidase Human genes 0.000 description 2
- 108010004098 Leucyl aminopeptidase Proteins 0.000 description 2
- 102000057297 Pepsin A Human genes 0.000 description 2
- 108090000284 Pepsin A Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229940111202 pepsin Drugs 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 208000006820 Arthralgia Diseases 0.000 description 1
- 208000008035 Back Pain Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241001333951 Escherichia coli O157 Species 0.000 description 1
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 description 1
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 241001670157 Gymnura Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 208000004296 neuralgia Diseases 0.000 description 1
- 208000024335 physical disease Diseases 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 208000020016 psychiatric disease Diseases 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
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Abstract
본 발명은 홍어 연골을 이용한 콘드로이틴 황산 소재 제조방법에 관한 것이다.
본 발명에 의한 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법은 홍어 가공 공정에서 발생된 홍어 연골이 분쇄되는 제 1단계(S100), 상기 제 1단계(S100)에서 분쇄된 상기 홍어 연골이 단백질 가수분해 처리되는 제 2단계(S200), 상기 제 2단계(S200)에서 가수분해 처리된 상기 홍어 연골 액체로 콘드로이틴 황산이 포함된 농축액이 생성되는 제 3단계(S300) 및 상기 제 3단계(S300)에서 생성된 상기 콘드로이틴 황산이 포함된 농축액을 정제하여 콘드로이틴 황산 소재가 제조되는 제 4단계(S400)를 포함하는 것을 특징으로 한다.The present invention relates to a method for producing chondroitin sulfate material using skate cartilage.
The method for producing high-purity chondroitin sulfate material using skate cartilage according to the present invention includes a first step (S100) in which skate cartilage generated in the skate processing process is pulverized, and the skate cartilage pulverized in the first step (S100) is subjected to protein hydrolysis. In the second step (S200), a concentrate containing chondroitin sulfate is produced from the skate cartilage liquid hydrolyzed in the second step (S200) and in the third step (S300). It is characterized in that it includes a fourth step (S400) in which chondroitin sulfate material is manufactured by purifying the produced concentrate containing chondroitin sulfate.
Description
본 발명은 홍어 연골을 이용한 콘드로이틴 황산 소재 제조방법에 관한 것으로, 보다 상세하게는 홍어 가공 중 부수적으로 발생되는 홍어 연골로부터 경제적이며 효율적인 공정을 통한 고순도 콘드로이틴 황산 소재의 제조방법에 관한 것이다.The present invention relates to a method for producing chondroitin sulfate material using skate cartilage, and more specifically, to a method for producing high purity chondroitin sulfate material through an economical and efficient process from skate cartilage incidentally generated during skate processing.
가오리과에 속하는 홍어(Skate)의 국내 어획량은 2021년을 기준으로 연간 약 3,800톤 이상이며, 홍어를 가공하고 남은 대량의 부산물로 연골, 껍질, 내장 등이 나오게 된다. 이 때 발생한 부산물은 홍어 특유의 냄새 등으로 인하여 다른 용도로 사용되기 어려워 다량 폐기되고 있는 실정이다.As of 2021, the domestic catch of skate, which belongs to the stingray family, is approximately 3,800 tons per year, and cartilage, skin, and intestines are produced as a large amount of by-products remaining after processing skate. The by-products generated at this time are difficult to use for other purposes due to the unique odor of skates, and are being discarded in large quantities.
그 중 홍어 연골에 함유되어 있는 점질성 뮤코다당인 콘드로이틴 황산은 인체에 있어서는 연결조직에 존재하여 콜라겐과 함께 세포간의 매트릭스의 주성분이 되고, 신경계 및 면역에도 관계하는 물질로서 관절염과 심신장애에도 효과적이라는 연구결과가 보고되고 있다. 실제 의약품에서는 신경통, 요통, 관절통 등 비교적 고령자의 많은 질병에 사용되고 있으며, 고령화가 진행됨에 따라 그 수요도 확대될 것이다. Among them, chondroitin sulfate, a viscous mucopolysaccharide contained in skate cartilage, is present in connective tissue in the human body and is a main component of the intercellular matrix along with collagen. It is also a substance related to the nervous system and immunity and is effective in arthritis and mental and physical disorders. Research results are being reported. In actual medicine, it is used to treat many diseases of relatively elderly people, such as neuralgia, back pain, and joint pain, and as the aging population progresses, demand will increase.
또한, 현재까지 콘드로이틴 황산의 주 원료로 소연골, 상어 연골이 사용되어 왔으나, 소연골 유래의 제품을 비롯한 육상포유동물 유래의 경우, 광우병, 구제역, 대장균 O-157 등의 오염 등으로 소비자의 기피가 우려되고, 상어 연골의 경우, 국제 상어 포획 금지 추세에 따라 원료의 공급이 원활하지 못한 실정이다.In addition, bovine cartilage and shark cartilage have been used as the main raw materials for chondroitin sulfate to date, but in the case of products derived from terrestrial mammals, including products derived from bovine cartilage, consumers are reluctant to use them due to contamination with mad cow disease, foot-and-mouth disease, and Escherichia coli O-157. There is concern, and in the case of shark cartilage, the supply of raw materials is not smooth due to the international trend of banning shark catching.
따라서, 폐기되고 있는 홍어 부산물인 연골을 재이용하여 콘드로이틴 황산 소재를 제조할 필요성이 있다.Therefore, there is a need to manufacture chondroitin sulfate material by reusing cartilage, which is a discarded skate by-product.
본 발명은 상기와 같은 문제점을 해결하기 위하여 안출된 것으로, 본 발명의 목적은 홍어 가공 공정에서 부수적으로 발생되는 홍어 연골을 이용한 고순도의 콘드로이틴 황산 소재의 제조방법을 제공하는데 있다.The present invention was created to solve the above problems, and the purpose of the present invention is to provide a method for producing high purity chondroitin sulfate material using skate cartilage incidentally generated during the skate processing process.
상기와 같은 기술적인 문제점을 해결하기 위하여, 본 발명에 의한 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법은 홍어 가공 공정에서 발생된 홍어 연골이 분쇄되는 제 1단계(S100), 상기 제 1단계(S100)에서 분쇄된 상기 홍어 연골이 단백질 가수분해 처리되는 제 2단계(S200), 상기 제 2단계(S200)에서 가수분해 처리된 상기 홍어 연골 액체로 콘드로이틴 황산이 포함된 농축액이 생성되는 제 3단계(S300) 및 상기 제 3단계(S300)에서 생성된 상기 콘드로이틴 황산이 포함된 농축액을 정제하여 콘드로이틴 황산 소재가 제조되는 제 4단계(S400)를 포함하는 것을 특징으로 한다.In order to solve the above technical problems, the method for producing high-purity chondroitin sulfate material using skate cartilage according to the present invention includes a first step (S100) in which skate cartilage generated in the skate processing process is pulverized, and the first step (S100). A second step (S200) in which the skate cartilage pulverized in step (S200) is subjected to protein hydrolysis, and a third step (S200) in which a concentrate containing chondroitin sulfate is produced from the skate cartilage liquid hydrolyzed in the second step (S200). S300) and a fourth step (S400) in which chondroitin sulfate material is produced by purifying the concentrate containing chondroitin sulfate produced in the third step (S300).
또한 바람직하게는 상기 제 1단계(S100)는 상기 홍어 연골이 80~100℃에서 15~25시간 가온(가열)된 후, 상기 홍어 연골에 잔존하는 연육이 제거되는 제 1-1단계(S110), 상기 제 1-1단계(S110)에서 상기 연육이 제거된 상기 홍어 연골이 열풍건조기에 의해 140~160℃에서 22~26시간 건조된 후 상온에서 보관되는 제 1-2단계(S120), 상기 제 1-2단계(S120)에서 건조된 상기 홍어 연골이 물에서 22~26시간 침지되는 제 1-3단계(S130) 및 상기 제 1-3단계(S130)에서 침지된 상기 홍어 연골이 분쇄되는 제 1-4단계(S140)를 포함하는 것을 특징으로 한다.Also preferably, the first step (S100) is a first step (S110) in which the skate cartilage is heated (heated) at 80 to 100° C. for 15 to 25 hours, and then the cartilage remaining in the skate cartilage is removed. , the 1-2 step (S120) in which the skate cartilage from which the surimi was removed in the 1-1 step (S110) is dried at 140-160 ° C. for 22-26 hours by a hot air dryer and then stored at room temperature. The skate cartilage dried in the first and second steps (S120) is immersed in water for 22 to 26 hours in the first and third steps (S130), and the skate cartilage soaked in the first and third steps (S130) is pulverized. It is characterized by including steps 1-4 (S140).
또한 바람직하게는 상기 제 2단계(S200)는 분쇄된 상기 홍어 연골에 분쇄된 상기 홍어 연골 무게의 4~6배의 물이 가수 처리되는 제 2-1단계(S210) 및 상기 제 2-1단계(S210)에서 가수 처리된 홍어 연골 액체가 단백질 분해효소에 의해 가수분해 처리되는 제 2-2단계(S220)를 포함하는 것을 특징으로 한다.Also preferably, the second step (S200) is a step 2-1 (S210) in which the pulverized skate cartilage is hydrotreated with water 4 to 6 times the weight of the pulverized skate cartilage. It is characterized in that it includes a 2-2 step (S220) in which the skate cartilage liquid hydrolyzed in (S210) is hydrolyzed by a proteolytic enzyme.
또한 바람직하게는 상기 제 3단계(S300)는 상기 제 2단계(S200)에서 가수분해 처리된 상기 홍어 연골 액체가 활성탄에 의해 탈취 처리되는 제 3-1단계(S310), 상기 제 3-1단계(S310)에서 탈취 처리된 상기 홍어 연골 액체를 가열하여 상기 홍어 연골 액체에서 단백질 분해효소가 실활 처리되는 제 3-2단계(S320), 상기 제 3-2단계(S320)에서 상기 단백질 분해효소가 실활 처리된 상기 홍어 연골 액체로부터 불순물을 제거하기 위해 상기 홍어 연골 액체가 감압여과 처리되는 제 3-3단계(S330) 및 상기 제 3-3단계(S330)에서 감압여과 처리된 상기 홍어 연골 액체가 감압농축되어 콘드로이틴 황산이 포함된 농축액이 생성되는 제 3-4단계(S340)를 포함하는 것을 특징으로 한다.Also preferably, the third step (S300) is a 3-1 step (S310) in which the skate cartilage liquid hydrolyzed in the second step (S200) is deodorized with activated carbon. In step 3-2 (S320), the proteolytic enzyme in the skate cartilage liquid is deactivated by heating the skate cartilage liquid deodorized in (S310). In step 3-2 (S320), the proteolytic enzyme is Step 3-3 (S330) in which the skate cartilage liquid is subjected to reduced pressure filtration to remove impurities from the deactivated skate cartilage liquid, and the skate cartilage liquid subjected to reduced pressure filtration in step 3-3 (S330) It is characterized in that it includes a 3-4 step (S340) in which a concentrate containing chondroitin sulfate is produced by concentration under reduced pressure.
또한 바람직하게는 상기 제 4단계(S400)는 상기 제 3단계(S300)에서 생성된 상기 콘드로이틴 황산이 포함된 농축액이 에탄올에 의해 정제되는 제 4-1단계(S410) 및 상기 제 4-1단계(S410)에서 정제된 상기 콘드로이틴 황산이 포함된 농축액이 동결건조되어 콘드로이틴 황산 소재가 제조되는 제 4-2단계(S420)를 포함하는 것을 특징으로 한다.Also preferably, the fourth step (S400) is a step 4-1 (S410) in which the concentrate containing chondroitin sulfate produced in the third step (S300) is purified with ethanol. It is characterized in that it includes a 4-2 step (S420) in which the concentrate containing the chondroitin sulfate purified in (S410) is freeze-dried to produce a chondroitin sulfate material.
또한 바람직하게는 상기 단백질 분해효소는 0.5~1.5wt% 알칼라제와 0.5~1.5wt% 프로타멕스의 혼합물인 것을 특징으로 한다.Also preferably, the proteolytic enzyme is a mixture of 0.5 to 1.5 wt% Alcalase and 0.5 to 1.5 wt% Protamex.
또한 바람직하게는 상기 제 3-1단계(S310)는 상기 활성탄이 상기 홍어 연골 액체의 0.1~1wt%이고, 상기 홍어 연골 액체가 상기 활성탄을 이용하여 30~70℃에서 20~260분동안 교반 처리되는 것을 특징으로 한다.Also preferably, in the 3-1 step (S310), the activated carbon is 0.1 to 1 wt% of the skate cartilage liquid, and the skate cartilage liquid is stirred at 30 to 70 ° C. for 20 to 260 minutes using the activated carbon. It is characterized by being
또한 바람직하게는 상기 제 4-1단계(S410)는 상기 콘드로이틴 황산이 포함된 농축액과 상기 에탄올의 비율이 1:2의 중량비인 것을 특징으로 한다.Also preferably, the 4-1 step (S410) is characterized in that the weight ratio of the concentrate containing chondroitin sulfate and the ethanol is 1:2.
상기한 바와 같이 이루어진 본 발명의 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법에 따르면, 대량 폐기되고 있는 홍어 연골에서 고순도의 콘드로이틴 황산 소재를 경제적이며 효율적인 방법으로 제조할 수 있다.According to the method for producing high-purity chondroitin sulfate material using skate cartilage of the present invention as described above, high-purity chondroitin sulfate material can be produced economically and efficiently from skate cartilage, which is discarded in large quantities.
본 발명에 의해 기능성 식품소재나 의료용 소재 및 상품 등에 이용 가능한 순도 높은 콘드로이틴 황산 소재의 생산량이 증대될 것이며, 이는 고령화가 급속하게 진행되고 있는 사회에서 매우 높은 가치를 지닐 것으로 기대된다.The present invention will increase the production of high-purity chondroitin sulfate material that can be used in functional food materials, medical materials, and products, and is expected to have very high value in a rapidly aging society.
본 발명은 도면에 도시된 실시예를 참고로 설명되었으나 이는 예시적인 것에 불과하며, 당해 기술 분야에서 통상의 지식을 가진 자라면 이로부터 다양한 변형 및 균등한 다른 실시예가 가능하다는 점을 이해할 것이다. 따라서, 본 발명의 진정한 기술적 보호 범위는 첨부된 특허청구범위의 기술적 사상에 의하여 정해져야 할 것이다.The present invention has been described with reference to the embodiments shown in the drawings, but these are merely exemplary, and those skilled in the art will understand that various modifications and equivalent other embodiments are possible therefrom. Therefore, the true scope of technical protection of the present invention should be determined by the technical spirit of the attached patent claims.
도 1은 본 발명의 바람직한 일 실시예에 따른 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법에 대한 순서도이다.
도 2는 도 1에 도시된 홍어 연골이 분쇄되는 제 1단계(S100)에 대한 순서도이다.
도 3은 도 1 도시된 홍어 연골이 가수분해 처리되는 제 2단계(S200)에 대한 순서도이다.
도 4는 본 발명에 의한 실험예 2의 결과를 도시한 그래프이다.
도 5는 도 1에 도시된 콘드로이틴 황산이 포함된 농축액이 생성되는 제 3단계(S300)에 대한 순서도이다.
도 6은 도 1에 도시된 콘드로이틴 황산 소재가 제조되는 제 4단계(S400)에 대한 순서도이다.
도 7은 본 발명에 의한 실험예 4의 결과를 도시한 그래프이다.Figure 1 is a flowchart of a method for producing high-purity chondroitin sulfate material using skate cartilage according to a preferred embodiment of the present invention.
Figure 2 is a flow chart for the first step (S100) in which the skate cartilage shown in Figure 1 is pulverized.
Figure 3 is a flow chart for the second step (S200) in which the skate cartilage shown in Figure 1 is hydrolyzed.
Figure 4 is a graph showing the results of Experimental Example 2 according to the present invention.
Figure 5 is a flowchart of the third step (S300) in which the concentrate containing chondroitin sulfate shown in Figure 1 is produced.
Figure 6 is a flowchart of the fourth step (S400) in which the chondroitin sulfate material shown in Figure 1 is manufactured.
Figure 7 is a graph showing the results of Experimental Example 4 according to the present invention.
이하, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 본 발명의 기술적 사상을 용이하게 실시할 수 있을 정도로 상세히 설명하기 위하여, 본 발명의 실시예를 첨부한 도면을 참조하여 설명하기로 한다.Hereinafter, in order to explain the present invention in detail so that a person skilled in the art can easily implement the technical idea of the present invention, embodiments of the present invention will be described with reference to the accompanying drawings.
도 1은 본 발명의 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법에 대한 순서도이다.Figure 1 is a flowchart of the method for producing high-purity chondroitin sulfate material using skate cartilage of the present invention.
도 1을 참조하면, 본 발명은 홍어 가공 공정에서 발생된 홍어 연골이 분쇄되는 제 1단계(S100), 분쇄된 상기 홍어 연골이 단백질 가수분해 처리되는 제 2단계(S200), 가수분해 처리된 상기 홍어 연골 액체로 콘드로이틴 황산이 포함된 농축액이 생성되는 제 3단계(S300)와 생성된 상기 콘드로이틴 황산이 포함된 농축액을 정제하여 콘드로이틴 황산 소재가 제조되는 제 4단계(S400)를 포함할 수 있다.Referring to Figure 1, the present invention includes a first step (S100) in which the skate cartilage generated in the skate processing process is pulverized, a second step (S200) in which the pulverized skate cartilage is subjected to protein hydrolysis, and the hydrolysis-treated It may include a third step (S300) in which a concentrate containing chondroitin sulfate is produced from skate cartilage liquid and a fourth step (S400) in which chondroitin sulfate material is produced by purifying the produced concentrate containing chondroitin sulfate.
도 2는 도 1에 도시된 홍어 연골이 분쇄되는 제 1단계(S100)에 대한 순서도이다.Figure 2 is a flow chart for the first step (S100) in which the skate cartilage shown in Figure 1 is pulverized.
도 2를 참조하면, 상기 제 1단계(S100)는 상기 홍어 연골이 가온(가열)된 후, 상기 홍어 연골에 잔존하는 연육이 제거되는 제 1-1단계(S110), 상기 연육이 제거된 상기 홍어 연골이 열풍건조기에 의해 건조된 후 상온에서 보관되는 제 1-2단계(S120), 건조된 상기 홍어 연골이 물에서 22~26시간 침지되는 제 1-3단계(S130)와 침지된 상기 홍어 연골이 분쇄되는 제 1-4단계(S140)를 포함할 수 있다.Referring to Figure 2, the first step (S100) is a first step (S110) in which the surimi remaining in the skate cartilage is removed after the skate cartilage is warmed (heated), and the surimi is removed. Step 1-2 (S120) in which skate cartilage is dried by a hot air dryer and stored at room temperature; Step 1-3 (S130) in which the dried skate cartilage is immersed in water for 22 to 26 hours and the immersed skate It may include steps 1-4 (S140) in which the cartilage is pulverized.
상기 제 1-1단계(S110)에서, 상기 홍어 연골은 80~100℃에서 15~25시간 가온(가열)될 수 있고, 상기 연육은 가온 자켓이 설치된 리본믹스(Riboon type mixer)를 이용하여 제거될 수 있다.In step 1-1 (S110), the skate cartilage can be heated (heated) at 80 to 100°C for 15 to 25 hours, and the surimi is removed using a ribbon mixer equipped with a heating jacket. It can be.
상기 제 1-2단계(S120)에서, 상기 홍어 연골은 열풍건조기에 의해 140~160℃에서 22~26시간 건조될 수 있다.In step 1-2 (S120), the skate cartilage may be dried at 140-160°C for 22-26 hours using a hot air dryer.
또한, 상기 제 1-3단계(S130)에서, 침지된 상기 홍어 연골은 수분을 충분히 흡수하여 상기 제 1-4단계(S140)를 용이하게 한다.Additionally, in step 1-3 (S130), the immersed skate cartilage sufficiently absorbs moisture to facilitate step 1-4 (S140).
도 3은 도 1에 도시된 홍어 연골이 가수분해 처리되는 제 2단계(S200)에 대한 순서도이다.Figure 3 is a flowchart of the second step (S200) in which the skate cartilage shown in Figure 1 is hydrolyzed.
도 3을 참조하면, 상기 제 2단계(S200)는 분쇄된 상기 홍어 연골에 분쇄된 상기 홍어 연골 무게의 4~6배의 물이 가수 처리되는 제 2-1단계(S210)와 상기 제 2-1단계(S210)에서 가수 처리된 홍어 연골 액체가 단백질 분해효소(protease)에 의해 가수분해 처리되는 제 2-2단계(S220)를 포함할 수 있다.Referring to Figure 3, the second step (S200) is a step 2-1 (S210) in which the pulverized skate cartilage is hydrotreated with water 4 to 6 times the weight of the pulverized skate cartilage, and the second- It may include a second-second step (S220) in which the skate cartilage liquid hydrolyzed in step 1 (S210) is hydrolyzed by protease.
여기서 단백질 분해효소는 알칼라제(Alcalase, Al)와 프로타멕스(Protamex, Pro)를 혼합한 것이다.Here, the proteolytic enzyme is a mixture of Alcalase (Al) and Protamex (Pro).
(실시예 1)(Example 1)
실험예 1: 가수량에 따라 제조된 콘드로이틴 황산 수율Experimental Example 1: Chondroitin sulfate yield prepared according to water content
상기 제 2-1단계(S210)에서 분쇄된 상기 홍어 연골 중량 대비 가수 처리되는 물의 양에 따라 제조된 콘드로이틴 황산(Chondroitin sulfate, CHS)의 수율을 각각 일반적인 산업에서 이용되는 수율 측정 장치를 사용하여 측정 실험하였다.The yield of chondroitin sulfate (CHS) prepared according to the amount of water hydrolyzed relative to the weight of the skate cartilage pulverized in step 2-1 (S210) was measured using a yield measuring device used in general industry. Experimented.
상기 표 1은 가수량에 따라 제조된 콘드로이틴 황산 소재 중의 콘드로이틴 황산 함량을 측정한 결과이다. 가수량이 증가함에 따라 제조된 콘드로이틴 황산 소재의 수율 또한 증가한다는 것을 알 수 있다.Table 1 above shows the results of measuring the chondroitin sulfate content in the chondroitin sulfate material prepared according to the water content. It can be seen that as the amount of water increases, the yield of the prepared chondroitin sulfate material also increases.
하지만, 가수량이 증가함에 따라 이후 제거해야할 수분이 많아지기 때문에 전체적인 공정 처리 시간은 늘어나게 된다. 가수량을 제외한 모든 조건이 동일하다는 전체 하에서 가수량이 5배인 경우 감압농축 시간은 2~4시간이고 가수량이 10배인 경우 감압농축 시간은 8~10시간으로 최소 3배 이상의 차이가 나게 된다.However, as the amount of water increases, the amount of moisture that needs to be removed increases, so the overall processing time increases. Under the condition that all conditions except the amount of water are the same, if the amount of water is 5 times the reduced pressure concentration time is 2 to 4 hours, and if the amount of water is 10 times the reduced pressure concentration time is 8 to 10 hours, a difference of at least 3 times.
따라서, 가수량이 분쇄된 상기 홍어 연골 중량의 5배인 경우에 가장 경제적이고 효율적으로 콘드로이틴 황산을 얻을 수 있음을 알 수 있다.Therefore, it can be seen that chondroitin sulfate can be obtained most economically and efficiently when the water content is 5 times the weight of the pulverized skate cartilage.
실험예 2: 단백질 분해효소에 따라 생성된 콘드로이틴 황산이 포함된 농축액 수율Experimental Example 2: Yield of concentrate containing chondroitin sulfate produced according to proteolytic enzyme
단백질 분해효소로서 알칼라제 2wt%, 프로타멕스 2wt%, 알칼라제 1wt%와 프로타멕스 1wt% 혼합물을 사용함에 따라 생성된 콘드로이틴 황산이 포함된 농축액의 수율을 각각 일반적인 산업에서 이용되는 수율 측정 장치를 사용하여 측정 실험하였다.The yield of the concentrate containing chondroitin sulfate produced by using Alcalase 2wt%, Protamex 2wt%, and a mixture of Alcalase 1wt% and Protamex 1wt% as proteolytic enzymes is the yield used in general industry. A measurement experiment was performed using a measuring device.
여기서, 콘드로이틴 황산이 포함된 농축액은 분쇄된 홍어 연골이 가수분해 처리된 후 감압여과 및 감압농축 처리된 상태이다.Here, the concentrate containing chondroitin sulfate is obtained by hydrolyzing crushed skate cartilage, followed by vacuum filtration and vacuum concentration.
도 4는 본 발명에 의한 실험예 2의 결과를 도시한 그래프이다.Figure 4 is a graph showing the results of Experimental Example 2 according to the present invention.
도 4를 참조하면, 단백질 분해효소로서 알칼라제 2wt%를 단독 사용한 경우 31.68%, 프로타멕스 2wt%를 단독 사용한 경우 31.49%의 수율을 보였으나, 두 단백질 분해효소를 혼합 사용한 경우 32.55%의 수율을 보였다.Referring to Figure 4, when 2 wt% of Alcalase was used alone as a proteolytic enzyme, the yield was 31.68%, and when 2 wt% of Protamex was used alone, the yield was 31.49%, but when the two proteolytic enzymes were mixed, the yield was 32.55%. Yield was shown.
실험예 3: 단백질 분해효소에 따라 제조된 콘드로이틴 황산 소재 순도Experimental Example 3: Purity of chondroitin sulfate material prepared according to proteolytic enzymes
단백질 분해효소로서 알칼라제 2wt%, 프로타멕스 2wt%, 알칼라제 1wt%와 프로타멕스 1wt% 혼합물을 사용함에 따라 제조된 콘드로이틴 황산 소재의 순도을 각각 일반적인 산업에서 이용되는 순도 측정 장치를 사용하여 측정 실험하였다.The purity of the chondroitin sulfate material produced by using Alcalase 2wt%, Protamex 2wt%, and a mixture of Alcalase 1wt% and Protamex 1wt% as proteolytic enzymes was measured using a purity measuring device used in general industry. A measurement experiment was conducted.
상기 표 2는 단백질 분해효소별 콘드로이틴 황산 소재 중의 단백질(Protein) 함량과 콘드로이틴 황산(Condroitin sulfate, CHS) 함량을 측정한 결과이다. 콘드로이틴 황산과 단백질의 함량은 분해효소 종류에 따라 평균 23.85%와 74.42%로 측정되었고, 그 비율은 1:2.8~3.2의 함량비로 나타났으며, 콘드로이틴 황산은 두 효소를 혼합 사용한 경우에 다소 높은 함량을 보였다.Table 2 above shows the results of measuring the protein content and chondroitin sulfate (CHS) content in the chondroitin sulfate material by proteolytic enzyme. The content of chondroitin sulfate and protein was measured on average at 23.85% and 74.42% depending on the type of decomposing enzyme, and the content ratio was 1:2.8 to 3.2, and the content of chondroitin sulfate was slightly higher when the two enzymes were mixed. showed.
실험예 2 및 실험예 3에 따르면, 알칼라제 1wt% 와 프로타멕스 1wt% 혼합물을 단백질 분해효소로 사용하여 가수분해 처리하였을 때 콘드로이틴 황산 소재의 순도가 가장 높다는 것을 알 수 있다.According to Experimental Examples 2 and 3, it can be seen that the purity of the chondroitin sulfate material was the highest when a mixture of 1wt% Alcalase and 1wt% Protamex was hydrolyzed using a proteolytic enzyme.
도 5는 도 1에 도시된 콘드로이틴 황산이 포함된 농축액을 생성하는 제 3단계(S300)에 대한 순서도이다.Figure 5 is a flow chart for the third step (S300) of producing a concentrate containing chondroitin sulfate shown in Figure 1.
도 5를 참조하면, 상기 제 3단계(S300)는 가수분해 처리된 상기 홍어 연골 액체가 활성탄에 의해 탈취 처리되는 제 3-1단계(S310), 탈취 처리된 상기 홍어 연골 액체를 가열하여 단백질 분해효소가 실활 처리되는 제 3-2단계(S320), 상기 단백질 분해효소가 실활 처리된 상기 홍어 연골 액체로부터 상기 홍어 연골 액체가 감압여과 처리되는 제 3-3단계(S330)와 감압여과 처리된 상기 홍어 연골 액체가 감압농축되어 콘드로이틴 황산이 포함된 농축액이 생성되는 제 3-4단계(S340)를 포함할 수 있다.Referring to Figure 5, the third step (S300) is a step 3-1 (S310) in which the hydrolyzed skate cartilage liquid is deodorized with activated carbon, and the deodorized skate cartilage liquid is heated to decompose the protein. Step 3-2 (S320) in which the enzyme is deactivated, Step 3-3 (S330) in which the skate cartilage liquid is subjected to reduced pressure filtration from the skate cartilage liquid in which the proteolytic enzyme has been deactivated, and the skate cartilage liquid is subjected to reduced pressure filtration. It may include a third or fourth step (S340) in which the skate cartilage liquid is concentrated under reduced pressure to produce a concentrate containing chondroitin sulfate.
상기 제 3-1단계(S310)에서, 상기 활성탄은 상기 홍어 연골 액체의 0.1~1wt%이고, 상기 홍어 연골 액체는 상기 활성탄에 의해 30~70℃에서 20~260분 동안 교반 처리될 수 있다.In the 3-1 step (S310), the activated carbon is 0.1 to 1 wt% of the skate cartilage liquid, and the skate cartilage liquid may be stirred with the activated carbon at 30 to 70 ° C. for 20 to 260 minutes.
또한, 상기 제 3-3단계(S330)에서, 감압여과 처리로 인해 상기 홍어 연골 액체의 불순물이 제거될 수 있다.Additionally, in step 3-3 (S330), impurities in the skate cartilage liquid may be removed through reduced pressure filtration.
그리고, 상기 제 3-4단계(S340)에서, 상기 홍어 연골 액체는 50~80℃에서 30~50˚Brix로 감압농축될 수 있다.And, in the 3-4 step (S340), the skate cartilage liquid may be concentrated under reduced pressure at 50 to 80°C to 30 to 50°Brix.
도 6은 1에 도시된 콘드로이틴 황산 소재를 제조하는 제 4단계(S400)에 대한 순서도이다.Figure 6 is a flow chart for the fourth step (S400) of manufacturing the chondroitin sulfate material shown in Figure 1.
도 6을 참조하면, 상기 제 4단계(S400)는 상기 제 3단계(S300)에서 생성된 상기 콘드로이틴 황산이 포함된 농축액이 에탄올에 의해 정제되는 제 4-1단계(S410)와 상기 제 4-1단계(S410)에서 정제된 상기 콘드로이틴 황산이 포함된 농축액이 동결건조되어 콘드로이틴 황산 소재가 제조되는 제 4-2단계(S420)를 포함할 수 있다.Referring to FIG. 6, the fourth step (S400) is a step 4-1 (S410) in which the concentrate containing chondroitin sulfate produced in the third step (S300) is purified by ethanol, and the fourth step (S410) It may include a 4-2 step (S420) in which the concentrate containing the chondroitin sulfate purified in step 1 (S410) is freeze-dried to produce chondroitin sulfate material.
상기 제 4-1단계(S410)에서, 상기 에탄올은 90~100w/w%이고 상기 콘드로이틴 황산이 포함된 농축액은 용매인 상기 에탄올과 3시간까지 접촉 교반될 수 있다.In step 4-1 (S410), the ethanol content is 90 to 100 w/w%, and the concentrate containing chondroitin sulfate may be contacted with the ethanol solvent and stirred for up to 3 hours.
여기서, 콘드로이틴 황산이 포함된 농축액은 다당체인 콘드로이틴 황산과 단백질로 구성되어 있는데, 에탄올 정제 시 단백질의 일부가 에탄올에 용해되어 함께 제거되기에 콘드로이틴 황산의 함량이 높아지게 된다.Here, the concentrate containing chondroitin sulfate is composed of chondroitin sulfate, a polysaccharide, and protein. During ethanol purification, part of the protein is dissolved in ethanol and removed together, so the content of chondroitin sulfate increases.
실험예 4: 용매비별 에탄올 정제 효과Experimental Example 4: Ethanol purification effect by solvent ratio
상기 제 4-1단계(S410)에서 홍어 연골에 홍어 연골 중량 대비 알칼라제를 2wt% 사용하거나 알칼라제와 프로타멕스를 각각 1wt%씩 혼합사용하여 가수분해하고 농축한 40°Brix의 시료에 에탄올 용매비를 달리하여 2시간 추출정제한 후 정제된 콘드로이틴 황산함량을 일반적으로 산업현장에서 사용되는 측정 장치를 이용하여 측정 실험하였다.In step 4-1 (S410), a sample of 40°Brix was hydrolyzed and concentrated by using 2 wt% of Alcalase or a mixture of Alcalase and Protamex of 1 wt% each based on the weight of skate cartilage in skate cartilage. After extraction and purification for 2 hours using different ethanol solvent ratios, the purified chondroitin sulfate content was measured using a measuring device generally used in industrial fields.
도 7은 본 발명에 의한 실험예 4의 결과를 도시한 그래프이다.Figure 7 is a graph showing the results of Experimental Example 4 according to the present invention.
도 7을 참조하면, 알칼라제만 독립적으로 사용하여 가수분해한 시료는 에탄올과 1:1의 중량비로 혼합하여 정제한 시료에서 42.92%로 가장 높은 함량을 나타내었고, 알칼라제와 프로타멕스를 각각 1wt%씩 혼합하여 가수분해한 시료에 대해서는 시료의 2배 중량의 에탄올을 사용하여 정제한 시료가 45.08%로 가장 높은 함량을 나타내었다.Referring to Figure 7, the sample hydrolyzed using only Alcalase independently showed the highest content of 42.92% in the sample purified by mixing with ethanol at a weight ratio of 1:1, and Alcalase and Protamex showed the highest content of 42.92%. For samples hydrolyzed by mixing 1 wt% of each, the sample purified using twice the weight of ethanol showed the highest content at 45.08%.
따라서, 알칼라제와 프로타멕스를 각각 1wt%씩 혼합하여 사용하는 본 발명에서 상기 콘드로이틴 황산이 포함된 농축액과 상기 에탄올의 비율이 1:2의 중량비인 것이 가장 순도 높은 콘드로이틴 황산 소재를 제조하는 방법임을 알 수 있다.Therefore, in the present invention, where Alcalase and Protamex are mixed at 1 wt% each, the weight ratio of the concentrate containing chondroitin sulfate and the ethanol is 1:2 to produce the highest purity chondroitin sulfate material. You can see that this is the method.
(실시예 2)(Example 2)
실시예 2는 상기 제 2-2단계(S220)에서 사용된 단백질 분해효소 혼합물을 제외한 모든 과정 및 제조방법이 본 발명과 동일하다. 상기 단백질 분해효소 혼합물은 펩신(Pepsin) 0.05~1.5wt%, 로이신아미노펩티다아제(Leucineaminopeptidase) 0.05~1.5wt%, 알칼라제 0.5~1.5wt% 및 프로타멕스 0.5~1.5wt%로 이루어질 수 있다.In Example 2, all processes and manufacturing methods are the same as those of the present invention except for the proteolytic enzyme mixture used in step 2-2 (S220). The proteolytic enzyme mixture may consist of 0.05 to 1.5 wt% of pepsin, 0.05 to 1.5 wt% of leucineaminopeptidase, 0.5 to 1.5 wt% of alcalase, and 0.5 to 1.5 wt% of Protamex.
실험예 5: 단백질 분해효소 혼합물에 따라 제조된 콘드로이틴 황산 소재 순도Experimental Example 5: Purity of chondroitin sulfate material prepared according to proteolytic enzyme mixture
단백질 분해효소로서 제 1 혼합물, 제 2 혼합물을 사용함에 따라 제조된 콘드로이틴 황산 소재의 순도을 각각 일반적인 산업에서 이용되는 순도 측정 장치를 사용하여 측정 실험하였다.The purity of the chondroitin sulfate material prepared by using the first mixture and the second mixture as proteolytic enzymes was measured using a purity measuring device used in general industry.
상기 제 1 혼합물은 알칼라제 1wt%와 프로타멕스 1wt% 혼합물이다.The first mixture is a mixture of 1 wt% Alcalase and 1 wt% Protamex.
상기 제 2 혼합물은 펩신 0.2wt%, 로이신아미노펩티다아제 0.2wt%, 알칼라아제 0.8wt% 및 프로타멕스 0.8wt% 혼합물이다.The second mixture is a mixture of 0.2 wt% pepsin, 0.2 wt% leucine aminopeptidase, 0.8 wt% alkalase, and 0.8 wt% Protamex.
상기 표 3은 단백질 분해효소 혼합물에 따른 콘드로이틴 황산 소재 중의 콘드로이틴 황산 함량을 측정한 결과이다. 콘드로이틴 황산의 함량은 제 1 혼합물을 사용하였을 때보다 제 2 혼합물을 사용하였을 때 최종적으로 더 높은 순도를 보였다.Table 3 above shows the results of measuring the chondroitin sulfate content in the chondroitin sulfate material according to the proteolytic enzyme mixture. The content of chondroitin sulfate ultimately showed higher purity when using the second mixture than when using the first mixture.
Claims (8)
상기 제 1단계(S100)에서 분쇄된 상기 홍어 연골이 단백질 가수분해 처리되는 제 2단계(S200);
상기 제 2단계(S200)에서 가수분해 처리된 상기 홍어 연골 액체로 콘드로이틴 황산이 포함된 농축액이 생성되는 제 3단계(S300); 및
상기 제 3단계(S300)에서 생성된 상기 콘드로이틴 황산이 포함된 농축액을 정제하여 콘드로이틴 황산 소재가 제조되는 제 4단계(S400); 를 포함하는 것을 특징으로 하는 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법.The first step (S100) in which skate cartilage generated during the skate processing process is pulverized;
A second step (S200) in which the skate cartilage pulverized in the first step (S100) is subjected to protein hydrolysis;
A third step (S300) in which a concentrate containing chondroitin sulfate is produced from the skate cartilage liquid hydrolyzed in the second step (S200); and
A fourth step (S400) in which chondroitin sulfate material is manufactured by purifying the concentrate containing chondroitin sulfate produced in the third step (S300); A method for producing a high-purity chondroitin sulfate material using skate cartilage, comprising:
상기 제 1단계(S100)는
상기 홍어 연골이 80~100℃에서 15~25시간 가온(가열)된 후, 상기 홍어 연골에 잔존하는 연육이 제거되는 제 1-1단계(S110);
상기 제 1-1단계(S110)에서 상기 연육이 제거된 상기 홍어 연골이 열풍건조기에 의해 140~160℃에서 22~26시간 건조된 후 상온에서 보관되는 제 1-2단계(S120);
상기 제 1-2단계(S120)에서 건조된 상기 홍어 연골이 물에서 22~26시간 침지되는 제 1-3단계(S130); 및
상기 제 1-3단계(S130)에서 침지된 상기 홍어 연골이 분쇄되는 제 1-4단계(S140);를 포함하는 것을 특징으로 하는 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법.According to clause 1,
The first step (S100) is
After the skate cartilage is heated (heated) at 80 to 100° C. for 15 to 25 hours, the surimi remaining in the skate cartilage is removed (S110);
A 1-2 step (S120) in which the skate cartilage from which the surimi has been removed in the 1-1 step (S110) is dried at 140 to 160° C. for 22 to 26 hours using a hot air dryer and then stored at room temperature;
A 1-3 step (S130) in which the skate cartilage dried in the 1-2 step (S120) is immersed in water for 22 to 26 hours; and
A method for producing high-purity chondroitin sulfate material using skate cartilage, comprising a step 1-4 (S140) in which the skate cartilage immersed in step 1-3 (S130) is pulverized.
상기 제 2단계(S200)는
분쇄된 상기 홍어 연골에 분쇄된 상기 홍어 연골 무게의 4~6배의 물이 가수 처리되는 제 2-1단계(S210); 및
상기 제 2-1단계(S210)에서 가수 처리된 홍어 연골 액체가 단백질 분해효소에 의해 가수분해 처리되는 제 2-2단계(S220);를 포함하는 것을 특징으로 하는 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법.According to clause 1,
The second step (S200) is
Step 2-1 (S210) in which the pulverized skate cartilage is hydrotreated with water 4 to 6 times the weight of the pulverized skate cartilage; and
A high-purity chondroitin sulfate material using skate cartilage, comprising a step 2-2 (S220) in which the skate cartilage liquid hydrolyzed in step 2-1 (S210) is hydrolyzed by a proteolytic enzyme. Manufacturing method.
상기 제 3단계(S300)는
상기 제 2단계(S200)에서 가수분해 처리된 상기 홍어 연골 액체가 활성탄에 의해 탈취 처리되는 제 3-1단계(S310);
상기 제 3-1단계(S310)에서 탈취 처리된 상기 홍어 연골 액체를 가열하여 상기 홍어 연골 액체에서 단백질 분해효소가 실활 처리되는 제 3-2단계(S320);
상기 제 3-2단계(S320)에서 상기 단백질 분해효소가 실활 처리된 상기 홍어 연골 액체로부터 불순물을 제거하기 위해 상기 홍어 연골 액체가 감압여과 처리되는 제 3-3단계(S330); 및
상기 제 3-3단계(S330)에서 감압여과 처리된 상기 홍어 연골 액체가 감압농축되어 콘드로이틴 황산이 포함된 농축액이 생성되는 제 3-4단계(S340);를 포함하는 것을 특징으로 하는 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법.According to clause 1,
The third step (S300) is
A 3-1 step (S310) in which the skate cartilage liquid hydrolyzed in the second step (S200) is deodorized with activated carbon;
A 3-2 step (S320) in which proteolytic enzymes in the skate cartilage liquid are deactivated by heating the skate cartilage liquid deodorized in the 3-1 step (S310);
A 3-3 step (S330) in which the skate cartilage liquid is subjected to reduced pressure filtration to remove impurities from the skate cartilage liquid in which the proteolytic enzyme has been deactivated in the 3-2 step (S320); and
Skate cartilage comprising a 3-4 step (S340) in which the skate cartilage liquid subjected to reduced pressure filtration in the 3-3 step (S330) is concentrated under reduced pressure to produce a concentrate containing chondroitin sulfate. Method for producing high-purity chondroitin sulfate material.
상기 제 4단계(S400)는
상기 제 3단계(S300)에서 생성된 상기 콘드로이틴 황산이 포함된 농축액이 에탄올에 의해 정제되는 제 4-1단계(S410); 및
상기 제 4-1단계(S410)에서 정제된 상기 콘드로이틴 황산이 포함된 농축액이 동결건조되어 콘드로이틴 황산 소재가 제조되는 제 4-2단계(S420);를 포함하는 것을 특징으로 하는 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법.According to clause 1,
The fourth step (S400) is
Step 4-1 (S410) in which the concentrate containing chondroitin sulfate produced in the third step (S300) is purified with ethanol; and
High purity using skate cartilage, comprising a 4-2 step (S420) in which the concentrate containing the chondroitin sulfate purified in the 4-1 step (S410) is freeze-dried to produce chondroitin sulfate material. Chondroitin sulfate material manufacturing method.
상기 단백질 분해효소는
0.5~1.5wt%의 알칼라제와 0.5~1.5wt%의 프로타멕스의 혼합물인 것을 특징으로 하는 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법.According to clause 3,
The proteolytic enzyme is
A method of manufacturing high-purity chondroitin sulfate material using skate cartilage, characterized in that it is a mixture of 0.5 to 1.5 wt% of Alcalase and 0.5 to 1.5 wt% of Protamex.
상기 제 3-1단계(S310)에서는
상기 활성탄이 상기 홍어 연골 액체의 0.1~1wt%이고, 상기 홍어 연골 액체가 상기 활성탄을 이용하여 30~70℃에서 20~260분 동안 교반 처리되는 것을 특징으로 하는 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법.According to clause 4,
In step 3-1 (S310),
Manufacturing of high-purity chondroitin sulfate material using skate cartilage, characterized in that the activated carbon is 0.1 to 1 wt% of the skate cartilage liquid, and the skate cartilage liquid is stirred for 20 to 260 minutes at 30 to 70 ° C. using the activated carbon. method.
상기 제 4-1단계(S410)에서는
상기 콘드로이틴 황산이 포함된 농축액과 상기 에탄올의 비율이 1:2의 중량비인 것을 특징으로 하는 홍어 연골을 이용한 고순도 콘드로이틴 황산 소재 제조방법.According to clause 5,
In step 4-1 (S410),
A method for producing high-purity chondroitin sulfate material using skate cartilage, characterized in that the ratio of the concentrate containing chondroitin sulfate and the ethanol is 1:2 by weight.
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