KR20230129826A - Composition for Skin-lightening and Improving Wrinkles Using Water-Soluble Curcumin and Wheat Bran Extracts - Google Patents

Abstract

The present invention provides skin whitening and improvement of skin wrinkles using water-soluble curcumin, wheat bran extract, or a mixture thereof, which inhibits the activity of tyronase in a concentration-dependent manner and also has the activity of inhibiting collagen biosynthesis in human fibroblasts in a concentration-dependent manner. Discloses a composition for

Description

Composition for skin-lightening and Improving Wrinkles Using Water-Soluble Curcumin and Wheat Bran Extracts}

The present invention relates to a composition for skin whitening and skin wrinkle improvement using water-soluble curcumin and wheat bran extract.

With the development of medical technology and the improvement of living standards, people's desire for beauty is growing stronger, and the perception that appearance itself is a form of social competitiveness is increasing. As a result, preferences for skin whitening and skin wrinkle improvement are increasing, and the cosmetics market is expanding (J .Korean Soc. Food Sci. Nutr., 42(3):355 (2013)).

The skin makes up about 16% of the human body, and is in direct contact with the external environment, playing an important role in protecting the human body from external harmful factors such as temperature, humidity, and ultraviolet rays. The skin is composed of the epidermis, dermis, and subcutaneous tissue under the keratin. The epidermis is a thin protective layer composed mainly of keratinocytes, which are made of keratin, melanocytes that produce and secrete melanin, immune cells such as Langerhans cells, and sensory-related cells (Merkel's copuscles). It prevents the invasion of external stimuli and pathogens, regulates body temperature, and maintains moisture and lipid components.

Melanin is a phenolic polymer natural pigment that is widely present in living organisms. In the human body, it is synthesized in melanosomes within melanocytes in the basal layer of the epidermis and is transferred to surrounding keratinocytes, giving the human skin color. If melanin is abnormally low, it causes skin lesions such as vitiligo. Conversely, if it is produced in excess, it causes acquired hyperpigmentation such as melasma, postinflammatory melanoderma, and solar lentigo. It can cause various skin diseases, including.

The synthesis of melanin is accomplished by creating melanosomes in melanocytes present in the basal layer of the skin, and the series of processes by which melanin is synthesized are collectively called melanogenesis. Melanin production uses tyrosine, one of the amino acids, as a substrate, and DOPA (3,4- dihydroxyphenylalanine) is converted to DOPA quinone, and then undergoes a non-enzymatic reaction and spontaneous oxidation process, and then melanin is synthesized through a polymerization reaction with amino acids or proteins (Korean J. FOOD SCI. TECHNOL., 2000. 32(3):736; Journal of Life Science, 2013. 23(12):1445; Pigment Cell Res.1999. 12(1):4-12.). Recently, research has been conducted on the inhibition of melanin synthesis using proteins such as tyrosinase, TRP-1, and TRP-2, as well as MITF (microphtalmia associated transcription factor). The main intracellular signaling pathway is the cAMP/PKA (cyclic monophosphate/protein kinase A) pathway. cAMP promotes the expression of MITF via PKA and CREB1 (cAMP responsive element binding protein 1), and MITF is important in the melanin synthesis process. As a transcriptional regulator, it promotes the transcription of tyrosinase, TRP-1, and TRP-2 (Pharmazie. 2015 Oct;70(10):646-9.; Int J Mol Sci. 2015 Oct 13;16(10):24219- 42; Curr Pharm Biotechnol. 2015;16(12):1111-9.).

Since tyrosinase plays the most important role in the first step of producing melanin, research on whitening agents mainly focuses on substances that inhibit tyrosinase activity or antagonists of tyrosinase substrates in order to control tyrosinase activity. Research has been conducted in the direction of developing (Kor. J. Pharmacogn., 44(3):220, 2013).

The skin makes up about 16% of the human body, and is in direct contact with the external environment, playing an important role in protecting the human body from external harmful factors such as temperature, humidity, and ultraviolet rays. The skin is composed of the epidermis, dermis, and subcutaneous tissue under the keratin. The epidermis is a thin protective layer composed mainly of keratinocytes, which are made of keratin, melanocytes that produce and secrete melanin, immune cells such as Langerhans cells, and sensory-related cells (Merkel's copuscles). It prevents the invasion of external stimuli and pathogens, regulates body temperature, and maintains moisture and lipid components. The dermis is a connective tissue beneath the epidermis and is mostly composed of a network structure of macromolecules called extracellular matrix. This extracellular stroma is made from fibroblasts and consists of fibrous proteins such as collagen and elastin and polysaccharides such as hyaluronic acid.

As we age, skin cells are damaged due to various pollutants, strong ultraviolet rays, stress, and nutritional deficiencies, and cell proliferation is impaired, causing wrinkles, loss of elasticity, pigmentation, and keratinization in the skin. Gilchrest B.A., J. Am. Acad. Dermatol, 21, 610. 1989).

Collagen is the most important protein in maintaining skin moisture and skin flexibility and elasticity, making up 90% of the dermal layer (J Am Acad Dermatol, 17(4):610-613. 2001), and giving the skin strength and tension. This serves to protect the skin from external stimulation. In addition, elastin in the dermal layer accounts for about 2%, which is less than collagen, but plays a role in supporting collagen fibers. Many recent studies have reported that the deficiency, aggregation, and loss of elastin fibers play an important role in the skin wrinkle formation mechanism. (Weihermann AC et al., Int J Cosmet Sci. 2017 Jun;39(3):241-247; Mora Huertas AC et al., Biochimie. 2016 Sep-Oct;128-129:163-73).

Collagen and elastin are synthesized in fibroblasts and decomposed by collagenase or elastase. As we age or are exposed to ultraviolet rays, the function of fibroblasts and the number of cells decreases, resulting in a decrease in the amount of collagen or elastin synthesis and an increase in the action of collagenase or elastase, which decomposes them. Once the elasticity of the skin caused by collagen and elastin is reduced, it is very difficult to recover.

Currently, substances that inhibit skin wrinkle formation include retinoic acid and retinol (Simon C et al., J Invest Dermatol 98: 248-260. 1992; Elaine S et al., J Invest Dermatol 96: 975-978. 1991). Its effectiveness has been proven, and it contains dehydroepiandrosterone (Shin MH et al., J Invest Dermatol 124: 315-323.2005), ginsenoside Rg3 extracted from ginseng (Kim SW et al., J Soc Cosmet Scientists Korea 30: 221-225.2004), and flavonoids ( The wrinkle improvement and skin protection effects of various substances such as Francesco B et al., Int J Pharm 145: 87-94.1996), Sorbus pear extract (Korean Patent No. 101008833), and lotus root extract (Korean Patent No. 100825450) were explored. It has been done. However, there are restrictions on the use of some substances because they have low stability or cause skin irritation, which poses safety issues. Accordingly, the search for substances with excellent anti-wrinkle activity and safety and stability continues.

The present invention discloses the tyrosinase inhibitory activity and collagen biosynthesis inhibitory activity of water-soluble curcumin and wheat bran extract.

The purpose of the present invention is to provide a composition for skin whitening using water-soluble curcumin and wheat bran extract.

Another object of the present invention is to provide a composition for improving skin wrinkles using water-soluble curcumin and wheat bran extract.

Another object or specific object of the present invention will be presented below.

The present invention, as confirmed in the examples and experimental examples below, shows that water-soluble curcumin, wheat bran extract, and mixtures thereof inhibit the activity of tyronesase in a concentration-dependent manner and also inhibit the biosynthesis of collagen in human fibroblasts in a concentration-dependent manner. It is completed by confirming suppression.

Considering the above, in one aspect, the present invention can be viewed as a composition for skin whitening containing water-soluble curcumin, wheat bran extract, or a mixture thereof as an active ingredient, and in another aspect, the present invention can be viewed as a composition for skin whitening containing water-soluble curcumin, wheat bran extract, or a mixture thereof as an active ingredient. It can be understood as a composition for improving skin wrinkles that uses the mixture as an active ingredient.

In this specification, water-soluble curcumin refers to a product obtained by suspending water-insoluble curcumin in water as a solvent (i.e., maintained in a dispersed state without coagulation or precipitation in water as a solvent). At this time, in order to stabilize the dispersed particles and prevent their aggregation and precipitation, suspending agents may be added in an appropriate amount before and after mixing curcumin with water. The suspending agent may be any known in the art, such as gum arabic, tragacanth gum, agar, karaya gum, locust bean gum, guar gum ( Water-soluble polymers such as guar gum, xanthan gum, ghatti gum, and pectin, cyclodextrin, glycerin or its fatty acid ester derivatives, methylcellulose, hydroxymethylcellulose, etc. Modified cellulose, various surfactants, etc. can be used. These suspending agents can be used alone or in a mixture of two or more, and can be used in an appropriate amount for suspension, specifically in the range of 2 to 100 parts by weight based on 100 weight of curcumin.

Also, in this specification, "wheat bran extract" refers to wheat bran, which is the object of extraction, with water, lower alcohols with 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate. , N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or extracts obtained by leaching using mixed solvents thereof, supercritical extraction solvents such as carbon dioxide and pentane. It refers to an extract obtained using or a fraction obtained by fractionating the extract, and the extraction method may be any method such as cold immersion, reflux, heating, ultrasonic radiation, or supercritical extraction, taking into account the polarity, degree of extraction, and degree of preservation of the active substance. It can be applied. In the case of a fractionated extract, the extract is suspended in a specific solvent and then mixed with a solvent of different polarity to form a fraction obtained by adsorbing the crude extract onto a column filled with silica gel and then mixed with a hydrophobic solvent, a hydrophilic solvent, or a mixture of these. It is meant to include fractions obtained using the mobile phase. In addition, the meaning of the extract includes concentrated liquid extract or solid extract from which the extraction solvent has been removed by methods such as freeze-drying, vacuum drying, hot air drying, and spray drying. Preferably, it refers to an extract obtained using water, ethanol, or a mixed solvent thereof as the extraction solvent, and more preferably, it refers to a water (hot water) extract as the extraction solvent.

Also, in this specification, “wheat bran” refers to a by-product obtained in the process of milling wheat. These by-products usually consist of the pericarp and germ, excluding the endosperm, which becomes flour.

In addition, in this specification, the meaning of “active ingredient” refers to an ingredient that can exhibit the desired activity alone or in combination with a carrier that is not active on its own.

The composition of the present invention may contain the active ingredient in any amount (effective amount) as long as it can exhibit a skin whitening effect, wrinkle improvement effect, etc. depending on the use, formulation, purpose of mixing, etc., and a typical effective amount is the total weight of the composition. As a standard, it will be determined within the range of 0.001% by weight to 15% by weight. Here, “effective amount” refers to the intended medical and cosmetic effects, such as skin whitening effect and wrinkle improvement effect, when the composition of the present invention is administered to mammals, preferably humans, to which it is applied during the administration period based on the suggestions of medical experts. It refers to the amount of the active ingredient contained in the composition of the present invention, which may indicate the like. Such effective amounts can be determined experimentally within the scope of the ordinary ability of those skilled in the art.

In addition to the active ingredients, the composition of the present invention has similar activities such as skin whitening effect or skin wrinkle improvement effect, etc., or skin hypersensitivity inhibition activity, skin protection activity (inhibition of skin damage caused by ultraviolet rays, skin moisturization, etc.). In order to improve the convenience of taking, ingesting, or using by adding, any compound or natural extract that has already been proven safe in the art and is known to have the corresponding activity may be additionally included.

These compounds or extracts include the Pharmacopoeia of each country (in Korea, "Korean Pharmacopoeia"), each country's Code of Health Functional Foods (in Korea, "Standards and Specifications for Health Functional Foods" notified by the Ministry of Food and Drug Safety), and the Code of Functional Cosmetics of each country ("Standards and Specifications for Health Functional Foods" notified by the Ministry of Food and Drug Safety in Korea). Compounds or extracts listed in compendia such as "Functional Cosmetics Standards and Test Methods"), compounds or extracts approved as items in accordance with each country's laws governing the manufacture and sale of pharmaceuticals (in Korea, the "Pharmaceutical Affairs Act"), and health functional foods. Compounds or extracts recognized as functional in accordance with the laws of each country that govern the manufacture and sale of functional cosmetics (the “Health Functional Foods Act” in Korea), and the laws of each country that govern the manufacture and sale of functional cosmetics (the “Cosmetics Act” in Korea). Accordingly, compounds or extracts with recognized functionality are included.

These ingredients include, for example, arbutin, niacinamide, ascorbyl glucoside, alpha-bisabolol, and oil-soluble ingredients recognized as skin whitening ingredients in the Cosmetics Code of Korea (Korea Ministry of Food and Drug Safety Notification, "Standards and Test Methods for Functional Cosmetics") in accordance with the Korean Cosmetics Act. Oil Soluble Licorice (Glycyrrhiza) Extract, etc., and retinol, retinyl palmitate, adenosine, polyethoxylated amide, etc., which are recognized as skin wrinkle improvement ingredients in the same Korean Cosmetic International, and ultraviolet rays in the same Korean Cosmetic International. Drometrizole, drometrizole trisiloxane, digalloyl trioleate, dimethylcodiethylbenzalmalonate, diethylhexylbutamidotriazone, etc. are recognized as protective ingredients, and are listed in Korea's "Act on Health Functional Foods". Accordingly, complexes such as pine bark extract, phosphatidylserine, finger root extract powder, and complex extracts such as red ginseng and Cornus officinalis, etc., which are recognized as UV protection ingredients, are also used to treat hypersensitive skin conditions according to the Korean “Health Functional Foods Act”. Recognized as improvement functional ingredients include L. sakei Probio 65, oil containing gamma-linolenic acid, L. plantarum CJLP133, a lactic acid bacteria derived from fruit and vegetables, and probiotic ATP. One or more of these compounds or natural extracts may be included in the composition of the present invention together with the active ingredient.

In a specific aspect, the composition of the present invention can be viewed as a food composition.

The food composition of the present invention can be manufactured in any form, for example, beverages such as tea, juice, carbonated beverages, and electrolyte drinks, processed oils such as milk and yogurt, gums, rice cakes, Korean snacks, bread, snacks, noodles, etc. It can be manufactured into health functional food preparations such as foods, tablets, capsules, pills, granules, liquids, powders, flakes, pastes, syrups, gels, jellies, bars, etc.

In addition, the food composition of the present invention can have any product classification in terms of legal and functional classification as long as it complies with the enforcement laws and regulations at the time of manufacture and distribution. For example, it is a health functional food according to the Korean 「Act on Health Functional Foods」, or confectionery, beans, tea, and beverages according to each food type according to the food code of the Korean 「Food Sanitation Act」 (Ministry of Food and Drug Safety Notification 「Food Standards and Specifications」) , special purpose food, etc.

The food composition of the present invention may contain food additives in addition to the active ingredients. Food additives can generally be understood as substances that are added to, mixed with, or infiltrated into food when manufacturing, processing, or preserving food. Since they are consumed daily and for a long period of time with food, their safety must be guaranteed. In the Food Additives Code of Laws of each country that regulates the manufacturing and distribution of food (in Korea, it is the Food Sanitation Act), food additives with guaranteed safety are limited in terms of ingredients or functions. In the Korea Food Additive Code (Ministry of Food and Drug Safety Notification “Food Additive Standards and Specifications”), food additives are classified into chemical synthetics, natural additives, and mixed preparations in terms of composition. These food additives are classified into sweeteners and flavors in terms of function. It is classified into preservatives, emulsifiers, acidulants, thickeners, etc.

Sweeteners are used to impart an appropriate sweetness to foods, and either natural or synthetic ones can be used in the composition of the present invention. Preferably, a natural sweetener is used, and natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose, and maltose.

Flavoring agents can be used to improve taste or aroma, and both natural and synthetic ones can be used. Preferably, natural products are used. When using natural products, they can serve the purpose of enhancing nutrition in addition to flavor. Natural flavoring agents may be obtained from apples, lemons, tangerines, grapes, strawberries, peaches, etc., or may be obtained from green tea leaves, coriander leaves, bamboo leaves, cinnamon, chrysanthemum leaves, jasmine, etc. You can also use things obtained from ginseng (red ginseng), bamboo shoots, aloe vera, and ginkgo nuts. Natural flavoring agents may be liquid concentrates or solid extracts. In some cases, synthetic flavoring agents may be used, and the synthetic flavoring agents may include esters, alcohols, aldehydes, terpenes, etc.

Preservatives include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), etc., and emulsifiers include acacia gum, carboxymethyl cellulose, xanthan gum, Examples include pectin, and acidulants include acidic acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, and phosphoric acid. In addition to improving taste, acidulants may be added to ensure that the food composition has an appropriate acidity for the purpose of suppressing the growth of microorganisms.

As a thickening agent, a suspending agent, settling agent, gel forming agent, bulking agent, etc. may be used.

In addition to the food additives described above, the food composition of the present invention may contain bioactive substances or minerals known in the art and whose safety is guaranteed as food additives for the purpose of supplementing and reinforcing functionality and nutrition.

Such physiologically active substances include catechins contained in green tea, vitamins such as vitamin B1, vitamin C, vitamin E, and vitamin B12, tocopherol, dibenzoylthiamine, etc., and minerals include calcium preparations such as calcium citrate and magnesium stearate. Magnesium preparations such as iron citrate, iron preparations such as chromium chloride, potassium iodine, selenium, germanium, vanadium, zinc, etc. are included.

The food composition of the present invention may contain the above-described food additives in an appropriate amount to achieve the purpose of addition depending on the product type.

Regarding other food additives that can be included in the food composition of the present invention, each country's food code or food additive code can be referred to.

The composition of the present invention may be considered a pharmaceutical composition in other specific embodiments. When the composition for skin whitening of the present invention is understood as a pharmaceutical composition, its use can be understood as a composition for improving skin hyperpigmentation.

The pharmaceutical composition of the present invention contains a pharmaceutically acceptable carrier in addition to the active ingredient and can be prepared into an oral formulation or a parenteral formulation depending on the route of administration by a conventional method known in the art. Here, the route of administration may be any suitable route, including topical route, oral route, intravenous route, intramuscular route, and direct absorption through mucosal tissue, and two or more routes may be used in combination. An example of a combination of two or more routes is a case where two or more dosage forms of drugs according to the administration route are combined, for example, when one drug is administered firstly through an intravenous route and the other drug is administered secondarily through a local route.

Pharmaceutically acceptable carriers are well known in the art depending on the route of administration or dosage form, and for specifics, reference can be made to each country's pharmacopoeia, including the "Korean Pharmacopoeia".

When the pharmaceutical composition of the present invention is prepared as an oral dosage form, it can be prepared as powder, granules, tablets, pills, dragees, capsules, solutions, gels, syrups, suspensions, and wafers using a suitable carrier according to methods known in the art. It can be manufactured in a dosage form such as: Examples of suitable carriers include sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol, and xylitol, starches such as corn starch, potato starch, and wheat starch, cellulose, methylcellulose, ethylcellulose, sodium carboxymethylcellulose, Cellulose such as hydroxypropylmethylcellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable oil, ethanol, glycerol. etc. can be mentioned. When formulating, appropriate binders, lubricants, disintegrants, colorants, diluents, etc. can be included as needed. Suitable binders include starch, magnesium aluminum silicate, starch paste, gelatin, methylcellulose, sodium carboxymethylcellulose, polyvinylpyrrolidone, glucose, corn sweetener, sodium alginate, polyethylene glycol, and wax. As a lubricant, sodium oleate is used. , sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, silica, talcum, stearic acid, its magnesium and calcium salts, polydethylene glycol, etc. Disintegrants include starch, methyl cellulose, Examples include agar, bentonite, xanthan gum, starch, alginic acid or its sodium salt. Additionally, diluents include lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine, etc.

When the pharmaceutical composition of the present invention is prepared as a parenteral formulation, it can be formulated in the form of injections, transdermal administration, nasal inhalation, and suppositories along with a suitable carrier according to methods known in the art. When formulated as an injection, an aqueous isotonic solution or suspension can be used as a suitable carrier. Specifically, an isotonic solution such as PBS (phosphate buffered saline) containing triethanolamine, sterile water for injection, or 5% dextrose can be used. . When formulated for transdermal administration, it can be formulated in the form of ointments, creams, lotions, gels, external solutions, paste preparations, linear preparations, and aerol preparations. In the case of nasal inhalation, it can be formulated in the form of an aerosol spray using suitable propellants such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, and carbon dioxide. When formulated as a suppository, the carrier is Wethepsol ( witepsol), Tween 61, polyethylene glycols, cocoa fat, laurel paper, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearates, sorbitan fatty acid esters, etc. can be used.

Regarding the specific formulation of pharmaceutical compositions, it is known in the art, and references can be made to, for example, Remington's Pharmaceutical Sciences (19th ed., 1995). The above documents are considered a part of this specification.

The preferred dosage of the pharmaceutical composition of the present invention is in the range of 0.001 mg/kg to 10 g/kg per day, preferably 0.001 mg/kg to 1 g, depending on the patient's condition, weight, gender, age, patient's severity, and administration route. It may be in the /kg range. Administration can be done once a day or divided into several times. These dosages should not be construed as limiting the scope of the invention in any respect.

In another specific aspect, the composition of the present invention can be considered a cosmetic composition.

Even if the composition of the present invention is identified as a cosmetic composition, the cosmetic composition may be classified as an arbitrary product in terms of its use and by law, and is specifically classified into functional cosmetics with uses such as improving skin troubles and atopic dermatitis, and non-functional cosmetics. It may be general cosmetics, etc. The product form can take any product form, specifically solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax. It can take the form of products such as foundation or spray. Specific product forms may include softening lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, or powder.

In addition to the active ingredient, the cosmetic composition of the present invention may contain ingredients commonly used in cosmetic compositions, such as stabilizers, solubilizers, surfactants, vitamins, conventional auxiliaries such as pigments and flavorings, and carriers.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent is used as a carrier component, specifically water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, etc. can be used.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, and microcrystals. Cellulose, aluminum metahydroxide, bentonite, agar, etc. can be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide. Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester can be used.

The cosmetic composition of the present invention can be prepared according to a cosmetic composition manufacturing method commonly used in the art, except that it contains active ingredients that exhibit skin whitening activity, etc.

As described above, according to the present invention, it is possible to provide a composition for skin whitening or improving skin wrinkles using water-soluble curcumin and wheat bran extract. The composition for skin whitening and the composition for improving skin wrinkles of the present invention can be commercialized as drugs, functional foods, etc.

Figures 1 to 3 show the results of evaluating tyrosinase inhibitory activity.
Figures 4 to 6 show the results of evaluating the degree of collagen biosynthesis.
Figure 7 shows the cytotoxicity evaluation results.

Hereinafter, the present invention will be described with reference to examples and experimental examples. However, the scope of the present invention is not limited to these examples and experimental examples.

<Example> Preparation of a mixture of water-soluble curcumin and wheat bran extract

Water-soluble curcumin was prepared using the method disclosed in Korean Patent No. 10-2177749 (name: Method for producing turmeric extract powder (curcumin) using turmeric and curcumin colloidal dispersion produced thereby). Specifically, 100 parts by weight of curcumin powder obtained by extracting turmeric was added to 3,000 parts by weight of purified water (based on 100 parts by weight of curcumin powder), then 0.3 parts by weight of xanthan gum (based on 100 parts by weight of curcumin powder) and 3.0 parts by weight of glycerin (based on 100 parts by weight of curcumin powder) were added. Water-soluble curcumin was prepared as a curcumin dispersion by adding (part by weight) and dispersing at a stirring speed of 4,500 rpm for 20 hours. A mixture of water-soluble curcumin and wheat bran extract was prepared by dissolving 5 parts by weight of wheat bran extract powder based on 100 parts by weight of the curcumin dispersion prepared in this way.

In the experiment below, the water-soluble curcumin and the mixture were concentrated under reduced pressure and freeze-dried to form a powder.

The wheat bran extract was obtained by adding 20 times the weight of water to wheat bran powder, extracting it by heating it at a temperature of 80 to 100 ° C. for 6 hours, and then concentrating it under reduced pressure and freeze-drying it to form a powder.

< Experiment example > Whitening activity and wrinkle improvement activity test

<Experimental Example 1> Whitening activity experiment

Whitening activity experiments were performed by measuring tyrosinase inhibitory activity. Specifically, 20 uL of samples of each concentration of the example and 20 uL of mushroom tyrosinase (125 U) were added to 60 uL of potassium phosphate buffer (pH 6.8) containing 10 mM L-DOPA as a substrate, and reacted at 37°C for 10 minutes. Then, the absorbance was measured at 492 nm using an absorptiophotometer. The results of tyrosinase inhibitory activity were calculated as a percentage compared to the untreated sample, and the results are shown in Figures 1 to 3.

<Experimental Example 2> Wrinkle improvement activity experiment

In the experiment, human dermal fibroblast neonatal cells (HDFn cells) were purchased and used, and the cells were grown in DMEM medium (Dulbeco's modified eagle's medium) with 10% FBS (fetal bovine serum) and 1% penicillin/streptomycin (100U/mL). mL) was added and cultured under 5% CO ₂ and 37°C conditions.

HDFn cells were distributed in 5 × 10 ⁴ cells in a 24-well plate and cultured at 5% CO ₂ at 37°C for 24 hours. Then, the medium was replaced with DMEM without FBS, samples were added, and cultured for 24 hours. The culture fluid was collected and the amount of collagen precursors produced was measured using a procollagen type I measurement kit (procollagen type I protein synthesis kit, Takara, Japan). The results are shown in Figures 4 to 6 as percentages compared to the untreated sample group.

Referring to Figures 4 to 6, each sample of the examples promoted collagen biosynthesis in a concentration-dependent manner, and in particular, the collagen biosynthesis promoting effect of the mixture of water-soluble curcumin and wheat bran extract was high.

Meanwhile, the cytotoxicity evaluation results of each example sample by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay (Br J Cancer 60, 206-210, 1989) are shown. It is shown in 7. Referring to FIG. 7, it can be seen that none of the samples of each example showed any particular cytotoxicity at a treatment concentration of 100 μg/mL.

Claims (6)

A composition for skin whitening comprising water-soluble curcumin, wheat bran extract, or a mixture thereof as an active ingredient.
According to paragraph 1,
The composition is characterized in that it is a food composition.
According to paragraph 1,
The composition is characterized in that it is a cosmetic composition.
A composition for improving skin wrinkles comprising water-soluble curcumin, wheat bran extract, or a mixture thereof as an active ingredient.
According to clause 4,
The composition is characterized in that it is a food composition.
According to clause 4,
The composition is characterized in that it is a cosmetic composition.