KR20230127412A - Composition having radical scavenging activity containing an extract of polar microalgae as an active ingredient - Google Patents
Composition having radical scavenging activity containing an extract of polar microalgae as an active ingredient Download PDFInfo
- Publication number
- KR20230127412A KR20230127412A KR1020220024393A KR20220024393A KR20230127412A KR 20230127412 A KR20230127412 A KR 20230127412A KR 1020220024393 A KR1020220024393 A KR 1020220024393A KR 20220024393 A KR20220024393 A KR 20220024393A KR 20230127412 A KR20230127412 A KR 20230127412A
- Authority
- KR
- South Korea
- Prior art keywords
- radical scavenging
- scavenging activity
- composition
- extract
- active ingredient
- Prior art date
Links
- 230000002292 Radical scavenging effect Effects 0.000 title claims abstract description 34
- 239000000284 extract Substances 0.000 title claims abstract description 24
- 239000000203 mixture Substances 0.000 title claims abstract description 18
- 239000004480 active ingredient Substances 0.000 title claims abstract description 16
- 230000005778 DNA damage Effects 0.000 claims 1
- 231100000277 DNA damage Toxicity 0.000 claims 1
- 208000027866 inflammatory disease Diseases 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 230000000968 intestinal effect Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 230000003449 preventive effect Effects 0.000 abstract description 2
- 235000016425 Arthrospira platensis Nutrition 0.000 description 3
- 240000002900 Arthrospira platensis Species 0.000 description 3
- 208000022559 Inflammatory bowel disease Diseases 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 229940082787 spirulina Drugs 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 2
- 239000003124 biologic agent Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000007358 intestinal barrier function Effects 0.000 description 2
- 230000003870 intestinal permeability Effects 0.000 description 2
- 238000001543 one-way ANOVA Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000016192 Demyelinating disease Diseases 0.000 description 1
- 206010012305 Demyelination Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 201000004681 Psoriasis Diseases 0.000 description 1
- 235000021068 Western diet Nutrition 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000004955 epithelial membrane Anatomy 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 208000026278 immune system disease Diseases 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000004941 influx Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 210000005027 intestinal barrier Anatomy 0.000 description 1
- 208000028774 intestinal disease Diseases 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 230000006371 metabolic abnormality Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 201000006417 multiple sclerosis Diseases 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000803 paradoxical effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/711—Natural deoxyribonucleic acids, i.e. containing only 2'-deoxyriboses attached to adenine, guanine, cytosine or thymine and having 3'-5' phosphodiester links
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Epidemiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Alternative & Traditional Medicine (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pain & Pain Management (AREA)
- Rheumatology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
본 발명은 극지 미세조류의 추출물을 유효성분으로 포함하는 라디칼 소거 활성을 갖는 조성물에 관한 것으로, 더욱 상세하게는 6 종의 미세조류 추출물을 유효성분으로 포함하는 H2O2- 및 OH 라디칼 소거 활성을 발휘하는 조성물을 제공하는 것에 관한 것이다.
본 발명에 따른 극지 미세조류의 추출물을 유효성분으로 포함하는 조성물은, 라디컬 소거 활성의 효과가 있고, 장관 염증질환 관련 예방 물질로 이용할 수 있는 효과가 있다.The present invention relates to a composition having radical scavenging activity comprising an extract of polar microalgae as an active ingredient, and more particularly, to a composition having H 2 O 2 - and OH radical scavenging activity containing extracts of six types of microalgae as an active ingredient. It relates to providing a composition that exhibits.
The composition containing the extract of polar microalgae according to the present invention as an active ingredient has the effect of radical scavenging activity and can be used as a preventive substance related to intestinal inflammatory diseases.
Description
본 발명은 극지 미세조류의 추출물을 유효성분으로 포함하는 라디칼 소거 활성(radical scavenging activity)을 갖는 조성물에 관한 것으로, 더욱 상세하게는 6 종의 미세조류 추출물을 유효성분으로 포함하는 H2O2- 및 OH 라디칼 소거 활성을 발휘하는 조성물을 제공하는 것에 관한 것이다.The present invention relates to a composition having radical scavenging activity comprising an extract of polar microalgae as an active ingredient, and more particularly, to a composition comprising extracts of 6 types of microalgae as an active ingredient, H 2 O 2 - and to providing a composition that exhibits OH radical scavenging activity.
최근 염증성 장질환 뿐 아니라 장관 외부의 질환에서도 장관 면역계 이상이 보고되고 있다. 건선은 염증성 장질환 동반 비율이 높으며 장관투과도 증가 및 마이크로바이옴 불균형이 확인되었다(JAMA Dermatol 154:1417). 다발성 경화증 중증도는 장관투과도와 유의하게 연관되어 있으며, 장점막 장벽기능 손상시 신경탈수초화가 증가한다(Brain 141:1900).Recently, intestinal immune system abnormalities have been reported not only in inflammatory bowel disease but also in diseases outside the intestinal tract. Psoriasis has a high comorbidity with inflammatory bowel disease, and increased intestinal permeability and microbiome imbalance have been confirmed (JAMA Dermatol 154:1417). Multiple sclerosis severity is significantly related to intestinal permeability, and nerve demyelination increases when intestinal barrier function is impaired (Brain 141:1900).
염증성 질환의 증가원인으로 서구식 식단의 유입 및 영양분의 과도한 섭취에 의한 장관 점막 손상 및 투과성 증가가 제시되고 있다. 대사이상, 심혈관질환 등의 합병증까지 고려하였을 때 염증성 질환 범주에 속하는 질환의 발병 빈도는 5명 중 1명에 달해, 향후 국가 보건의 중대한 위험 요소로 치료제 시장 규모는 세계적으로 60조원 이상으로 예상된다.Damage to the intestinal mucosa and increased permeability due to the influx of a Western diet and excessive intake of nutrients have been proposed as the cause of the increase in inflammatory diseases. Considering complications such as metabolic abnormalities and cardiovascular diseases, the frequency of occurrence of diseases belonging to the category of inflammatory diseases reaches 1 in 5 cases. .
염증성 장질환 치료에 비특이적 염증 억제제와 단일 표적 염증매개체를 억제하는 생물학적 제제가 이용되고 있지만, 발병과 관련된 단백질, 사이토카인, 대사체 등이 다양하기 때문에 하나의 대사과정이나 신호전달과정, 타깃 단백질 조절로 완벽한 치료효과를 획득하는 것은 어려운 실정이다.Biological agents that inhibit non-specific inflammation inhibitors and single-target inflammatory mediators are used for the treatment of inflammatory bowel disease. However, it is difficult to obtain a perfect therapeutic effect.
생물학적 제제는 70% 정도의 환자에서만 반응이 있고, 장기간 사용시 약물에 대한 항체 형성으로 치료효과가 감소하거나 장점막 장벽 유지에 필요한 정상 면역반응이 억제되어 역설적 장질환이 발생할 수 있다(N Engl J Med 376:1551, J Invest Dermatol 135:31).Biological agents respond to only about 70% of patients, and when used for a long time, the therapeutic effect may be reduced due to the formation of antibodies to the drug, or paradoxical intestinal disease may occur due to suppression of the normal immune response required to maintain the intestinal barrier (N Engl J Med 376 :1551, J Invest Dermatol 135:31).
피부와 장관의 염증 질환을 근원적으로 조절하기 위해서는 상피 및 점막의 장벽 기능 강화가 필수적이다.In order to fundamentally control inflammatory diseases of the skin and intestinal tract, it is essential to strengthen the barrier function of the epithelial and mucous membranes.
극지 생물은 극한의 환경에서 효율적으로 생존하기 위해 일반 생물체와 구별되는 세포보호물질을 생산할 것으로 예상되어, 장벽 구조물을 경계로 외부 환경에 직접 노출되어 있는 피부와 장관의 면역 불균형 조절을 위한 천연물 신물질로서 활용 가능성이 높아 다양한 연구가 필요한 실정이다.Polar organisms are expected to produce cell protective substances that are distinct from normal organisms in order to survive in extreme environments efficiently, and as a natural new substance for controlling immune imbalance in the skin and intestinal tract directly exposed to the external environment through barrier structures. There is a high possibility of utilization, and various studies are needed.
본 발명의 목적은 극지 미세조류의 추출물을 유효성분으로 포함하는 라디칼 소거 활성을 갖는 조성물을 제공하는 데 있다.An object of the present invention is to provide a composition having a radical scavenging activity comprising an extract of polar microalgae as an active ingredient.
상기 목적을 달성하기 위하여, 본 발명은 극지 미세조류의 추출물을 유효성분으로 포함하는 라디칼 소거 활성을 갖는 조성물을 제공한다.In order to achieve the above object, the present invention provides a composition having a radical scavenging activity comprising an extract of polar microalgae as an active ingredient.
상기 극지 미세조류의 추출물을 유효성분으로 포함하는 라디칼 소거 활성을 갖는 조성물은, 서열번호 1 내지 6으로 이루어진 군에서 선택된 1종 이상의 미세조류의 추출물을 유효성분으로 포함할 수 있다.The composition having radical scavenging activity including the extract of polar microalgae as an active ingredient may include extracts of one or more types of microalgae selected from the group consisting of SEQ ID NOs: 1 to 6 as an active ingredient.
상기 라디칼 소거 활성은 H2O2 라디칼 소거 활성 또는 OH 라디칼 소거 활성인 것일 수 있다.The radical scavenging activity may be H 2 O 2 radical scavenging activity or OH radical scavenging activity.
상기 OH 라디칼은 DNA를 손상시키는 것일 수 있다.The OH radical may damage DNA.
본 발명에 따른 극지 미세조류의 추출물을 유효성분으로 포함하는 조성물은, 라디컬 소거 활성의 효과가 있고, 장관 염증질환 관련 예방 물질로 이용할 수 있는 효과가 있다.The composition containing the extract of polar microalgae as an active ingredient according to the present invention has the effect of radical scavenging activity and can be used as a preventive substance related to intestinal inflammatory diseases.
도 1은 미세조류 추출물의 H2O2 라디칼 소거 활성능을 측정한 그래프이다.
도 2는 미세조류 추출물의 OH 라디칼 소거 활성능을 측정한 그래프이다.1 is a graph measuring the H 2 O 2 radical scavenging activity of microalgae extracts.
Figure 2 is a graph measuring the OH radical scavenging activity of the microalgae extract.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명의 일 형태에 따라 극지 미세조류의 추출물을 유효성분으로 포함하는 라디칼 소거 활성을 갖는 조성물을 제공한다.According to one aspect of the present invention, a composition having radical scavenging activity comprising an extract of polar microalgae as an active ingredient is provided.
상기 극지 미세조류의 추출물을 유효성분으로 포함하는 라디칼 소거 활성을 갖는 조성물은, 서열번호 1 내지 6으로 이루어진 군에서 선택된 1종 이상의 미세조류의 추출물을 유효성분으로 포함할 수 있다.The composition having radical scavenging activity including the extract of polar microalgae as an active ingredient may include extracts of one or more types of microalgae selected from the group consisting of SEQ ID NOs: 1 to 6 as an active ingredient.
상기 라디칼 소거 활성은 H2O2 라디칼 소거 활성 또는 OH 라디칼 소거 활성인 것일 수 있다.The radical scavenging activity may be H 2 O 2 radical scavenging activity or OH radical scavenging activity.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.
본 발명에서는 서열번호 1 내지 6 중에서 선택된 각각의 서열번호를 갖는 6 가지 극지 미세조류 KSF0041(서열번호 1), KNM0029C(서열번호 2), KSF0015(서열번호 3), KSF0037(서열번호 4), KSF0003(서열번호 5) 및 KSF0134(서열번호 6)에 대해 라디칼 소거 활성 기능을 검증하였다.In the present invention, six polar microalgae KSF0041 (SEQ ID NO: 1), KNM0029C (SEQ ID NO: 2), KSF0015 (SEQ ID NO: 3), KSF0037 (SEQ ID NO: 4), KSF0003 having respective SEQ ID NOs selected from SEQ ID NOS: 1 to 6 (SEQ ID NO: 5) and KSF0134 (SEQ ID NO: 6) were verified for radical scavenging activity.
실시예 1. 극지 미세조류 추출물의 HExample 1. H of polar microalgae extract 22 OO 22 라디칼 소거 활성 검증 Validation of radical scavenging activity
도 1은 미세조류 추출물의 H2O2 라디칼 소거 활성능을 측정한 그래프이다. H2O2 10 mM과 미세조류 추출물 5 ㎍을 10분간 반응시킨 후 DCHF-DA 형광 발색을 측정한 결과, 양성 대조군(positive control)인 스피루리나(spirulina)보다 라디칼 소거 활성이 우수하였다.1 is a graph measuring the H 2 O 2 radical scavenging activity of microalgae extracts. As a result of measuring DCHF-DA fluorescence after reacting 10 mM H 2 O 2 and 5 μg of microalgal extract for 10 minutes, the radical scavenging activity was superior to that of spirulina, a positive control.
*vs. spirulina ***P < 0.001, ****P < 0.0001 (oneway ANOVA)*vs. spirulina ***P < 0.001, ****P < 0.0001 (oneway ANOVA)
실시예 2. 극지 미세조류 추출물의 OH 라디칼 소거 활성 검증Example 2. Verification of OH radical scavenging activity of polar microalgae extract
도 2는 미세조류 추출물의 OH 라디칼 소거 활성능을 측정한 그래프이다. 펜톤 반응(fenton reaction)을 이용하여 DNA를 손상시키는 OH 라디칼을 생성시킨 후 미세조류 추출물 5 ㎍을 10분간 반응시킨 후 DCHF-DA 형광 발색을 측정한 결과, 6종의 미세조류 추출물 모두 스피루리나보다 OH 라디칼 소거 활성이 우수하였다.Figure 2 is a graph measuring the OH radical scavenging activity of the microalgae extract. After generating OH radicals that damage DNA using the Fenton reaction, 5 μg of microalgae extract was reacted for 10 minutes, and then DCHF-DA fluorescence was measured. The radical scavenging activity was excellent.
*vs. spirulina ***P < 0.001, ****P < 0.0001 (oneway ANOVA)*vs. spirulina ***P < 0.001, ****P < 0.0001 (oneway ANOVA)
이상, 본 발명내용의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적인 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의해 정의된다고 할 것이다.In the above, specific parts of the present invention have been described in detail, and for those skilled in the art, it is clear that these specific descriptions are only preferred embodiments, and the scope of the present invention is not limited thereby. something to do. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.
<110> Gachon University of Industry-Academic cooperation Foundation Korea Institute of Ocean Science & Technology <120> COMPOSITION HAVING RADICAL SCAVENGING ACTIVITY CONTAINING AN EXTRACT OF POLAR MICROALGAE AS AN ACTIVE INGREDIENT <130> P22-E022 <160> 6 <170> KoPatentIn 3.0 <210> 1 <211> 1636 <212> DNA <213> Unknown <220> <223> KSF0041 <400> 1 actgcgaatg gctcattaaa tcagttatag tttatttgat ggtacctact actcggatac 60 ccgtagtaaa tctagagcta atacgtgcgt aaatcccgac ttctggaagg gacgtattta 120 ttagataaaa ggccgaccgg gctctgcccg actcgcggtg aatcatgata acttcacgaa 180 tcgcatggcc ttgtgccggc gatgtttcat tcaaatttct gccctatcaa ctttcgatgg 240 taggatagag gcctaccatg gtggtaacgg gtgacggagg attagggttc gattccggag 300 agggagcctg agaaacggct accacatcca aggaaggcag caggcgcgca aattacccaa 360 tcctgacaca gggaggtagt gacaataaat aacaatactg ggccttttca ggtctggtaa 420 ttggaatgag tacaatctaa accccttaac gaggatcaat tggagggcaa gtctggtgcc 480 agcagccgcg gtaattccag ctccaatagc gtatatttaa gttgctgcag ttaaaaagct 540 cgtagttgga tttcgggtgg ggcctgccgg tccgccgttt cggtgtgcac tggcagggcc 600 caccttgttg ccggggacgg gctcctgggc ttcactgtcc gggactcgga gtcggcgctg 660 ttactttgag taaattagag tgttcaaagc aggcctacgc tctgaataca ttagcatgga 720 ataacacgat aggactctgg cctatcctgt tggtctgtag gaccggagta atgattaaga 780 gggacagtcg ggggcattcg tatttcattg tcagaggtga aattcttgga tttatgaaag 840 acgaactact gcgaaagcat ttgccaagga tgttttcatt aatcaagaac gaaagttggg 900 ggctcgaaga cgattagata ccgtcctagt ctcaaccata aacgatgccg actagggatc 960 ggcggatgtt tcttcgatga ctccgccggc accttatgag aaatcaaagt ttttgggttc 1020 cggggggagt atggtcgcaa ggctgaaact taaaggaatt gacggaaggg caccaccagg 1080 cgtggagcct gcggcttaat ttgactcaac acgggaaaac ttaccaggtc cagacatagt 1140 gaggattgac agattgagag ctctttcttg attctatggg tggtggtgca tggccgttct 1200 tagttggtgg gttgccttgt caggttgatt ccggtaacga acgagacctc agcctgctaa 1260 atagtcacga ttggctcgcc agtcggcgga cttcttagag ggactattgg cgactagcca 1320 atggaagcat gaggcaataa caggtctgtg atgcccttag atgttctggg ccgcacgcgc 1380 gctacactga tgcattcaac gagcttagcc ttggccgaga ggcccgggta atctttgaaa 1440 ctgcatcgtg atggggatag attattgcaa ttattaatct tcaacgagga atgcctagta 1500 agcgcaagtc atcagcttgc gttgattacg tccctgccct ttgtacacac cgcccgtcgc 1560 tcctaccgat tgggtgtgct ggtgaagtgt tcggattggc gactgggggc ggtctccgct 1620 ctcagccgcc gaaaag 1636 <210> 2 <211> 1705 <212> DNA <213> Unknown <220> <223> KNM0029C <400> 2 gcgaatggct cattaaatca gttatagttt atttgatggt acctctactc ggataaccgt 60 agtaattcta gagctaatac gtgcgcaaat cccgacttct ggaagggacg tatttattag 120 ataaaaggcc agccgagctt gctcgacctg cggtgactca taataacttc acgaatcgca 180 cggccttgcg ccggcgatgt ttcattcaaa tttctgccct atcaactttc gatggtagga 240 tagaggccta ccatggtggt aacgggtgac ggaggattag ggttcgattc cggagaggga 300 gcctgagaaa cggctaccac atccaaggaa ggcagcaggc gcgcaaatta cccaatcccg 360 acacggggag gtagtgacaa taaataacaa taccgggcgt tcaacgtctg gtaattggaa 420 tgagtacaat ttaaatccct taacgaggat ccattggagg gcaagtctgg tgccagcagc 480 cgcggtaatt ccagctccaa tagcgtatat ttaagttgct gcagttaaaa agctcgtagt 540 tggatttcgg gcgagtgacg acggtccgcc tctggtgtgc actgccgttc acccgccttt 600 ctgtcgggaa cgtgctcctg gcctttactg tctgggacac gcgttcgacg aggttacttt 660 gagtaaatta gagtgttcaa agcaggcatc gccttgaata tattagcatg gaatagcacg 720 ataggactct ggcctatttt gctggtctgt aggaccggag taatgattaa gagggacagt 780 cgggggcatt cgtatttcat tgtcagaggt gaaattcttg gatttatgaa agacgaactt 840 ctgcgaaagc atttgccaag gatgttttca ttgatcaaga acgaaagttg ggggctcgaa 900 gacgattaga taccgtcgta gtctcaacca taaacgatgc cgaccaggga ttaggcagtg 960 ttcttttgat gacctgccta gcaccttgag agaaatcaga gtttttgggt tccgggggga 1020 gtatggtcgc aaggctgaaa cttaaaggaa ttgacggaag ggcaccacca ggcgtggagc 1080 ctgcggctta atttgactca acacgggaaa acttaccagg tccagacacg gggatgataa 1140 acagattgag agctcttttt tgattctgtg ggtggtggtg catggccgtt cttagttggt 1200 gggttgcctt gtcaggttga ttccggtaac gaacgagacc tcagcctgct aaatagtcac 1260 atatcctttt tggatatgcc tgacttctta gagggactat tgtcgtttag gcaatggaag 1320 tgtgaggcaa taacaggtct gtgatgccct tagatgttct gggccgcacg cgcgctacac 1380 tgatgcattc aacgagccta tccttggcag aaatgcccgg gtaatctttg aaactgcatc 1440 gtgatgggga tagattattg caattattag tcttcaacga ggaatgccta gtaagcgcga 1500 gtcatcagct cgcgctgatt acgtccctgc cctttgtaca caccgcccgt cgctcctacc 1560 gattgggtgt gctggtgaga actgaggatt ggctgttatt ggtggcaaca ctgattatgg 1620 ccgagaacca gtttaaaccc tcccacctag aggaaggaga agtcgtaaca aggtttccgt 1680 aggtgaacct gcggaaggat cattg 1705 <210> 3 <211> 1609 <212> DNA <213> Unknown <220> <223> KSF0015 <400> 3 ctgcgaatgg ctcattaaat cagttatagt ttatttgatg gtacctacta ctcggatacc 60 cgtagtaaat ctagagctaa tacgtgcgta aatcccgact tctggaaggg acgtatttat 120 tagataaaag gccgaccggg ctctgcccga ctcgcggtga atcatgataa cttcacgaat 180 cgcatggcct tgtgccggcg atgtttcatt caaatttctg ccctatcaac tttcgatggt 240 aggatagagg cctaccatgg tggtaacggg tgacggagga ttagggttcg attccggaga 300 gggagcctga gaaacggcta ccacatccaa ggaaggcagc aggcgcgcaa attacccaat 360 cctgacacag ggaggtagtg acaataaata acaatactgg gccttttcag gtctggtaat 420 tggaatgagt acaatctaaa ccccttaacg aggatcaatt ggagggcaag tctggtgcca 480 gcagccgcgg taattccagc tccaatagcg tatatttaag ttgctgcagt taaaaagctc 540 gtagttggat ttcgggtggg gcctgccggt ccgccgtttc ggtgtgcact ggcagggccc 600 accttgttgc cggggacggg ctcctgggct tcactgtccg ggactcggag tcggcgctgt 660 tactttgagt aaattagagt gttcaaagca ggcctacgct ctgaatacat tagcatggaa 720 taacacgata ggactctggc ctatcctgtt ggtctgtagg accggagtaa tgattaagag 780 ggacagtcgg gggcattcgt atttcattgt cagaggtgaa attcttggat ttatgaaaga 840 cgaactactg cgaaagcatt tgccaaggat gttttcatta atcaagaacg aaagttgggg 900 gctcgaagac gattagatac cgtcctagtc tcaaccataa acgatgccga ctagggatcg 960 gcggatgttt cttcgatgac tccgccggca ccttatgaga aatcaaagtt tttgggttcc 1020 ggggggagta tggtcgcaag gctgaaactt aaaggaattg acggaagggc accaccaggc 1080 gtggagcctg cggcttaatt tgactcaaca cgggaaaact taccaggtcc agacatagtg 1140 aggattgaca gattgagagc tctttcttga ttctatgggt ggtggtgcat ggccgttctt 1200 agttggtggg ttgccttgtc aggttgattc cggtaacgaa cgagacctca gcctgctaaa 1260 tagtcacgat tggctcgcca gtcggcggac ttcttagagg gactattggc gactagccaa 1320 tggaagcatg aggcaataac aggtctgtga tgcccttaga tgttctgggc cgcacgcgcg 1380 ctacactgat gcattcaacg agcttagcct tggccgagag gcccgggtaa tctttgaaac 1440 tgcatcgtga tggggataga ttattgcaat tattaatctt caacgaggaa tgcctagtaa 1500 gcgcaagtca tcagcttgcg ttgattacgt ccctgccctt tgtacacacc gcccgtcgct 1560 cctaccgatt gggtgtgctg gtgaagtgtt cggattggcg actgggggc 1609 <210> 4 <211> 1646 <212> DNA <213> Unknown <220> <223> KSF0037 <400> 4 actgcttata ctgtgaaact gcgaatggct cattaaatca gttatagttt atttgatggt 60 acctactact cggataaccg tagtaattct agagctaata cgtgcgcaca acccgacttc 120 tggaagggtc gtatttatta gataaaaggc cagccgggct ctgcccgacc tgcggtgaat 180 catgataact tcacgaatcg tatggcctcg cgccgacgat gtttcattca aatttctgcc 240 ctatcaactt tcgatggtag gatagaggcc taccatggtg gtaacgggtg acggaggatt 300 agggttcgat tccggagagg gagcctgaga gatggctacc acatccaagg aaggcagcag 360 gcgcgcaaat tacccaatcc cgacacgggg aggtagtgac aataaataac aataccgggc 420 gcttcgcgtc tggtaattgg aatgagtaca atctaaatcc cttaacgagg atccattgga 480 gggcaagtct ggtgccagca gccgcggtaa ttccagctcc aatagcgtat atttaagttg 540 ttgcagttaa aaagctcgta gttggatttc gggtgggacg gtgcggtccg cctctggtgt 600 gcactgctct gttccacctt cctgccgggg acgggctcct gggcttcact gtctgggact 660 cggagtcggc gaggttactt tgagtaaatt agagtgttca aagcaggcct acgctctgaa 720 tacattagca tggaataaca cgataggact ctggcctatc tgttggtctg tgggaccgga 780 gtaatgatta agaggggtag tcggggacat tcgtattccg ttgtcagagg tgaaattctt 840 ggatttacgg aagacgaaca tctgcgaaag catttgtcaa ggatactttc attgatcaag 900 aacgaaagtt gggggctcga agacgattag ataccgtcgt agtctcaacc ataaacgatg 960 ccgactaggg attggcagat gttcttttga tgactctgcc agcaccttat gagaaatcaa 1020 agtttttggg ttccgggggg agtatggtcg caaggctgaa acttaaagga attgacggaa 1080 gggcaccacc aggcgtggag cctgcggctt aatttgactc aacacgggga aacttaccag 1140 gtccagacac gggaaggatt gacagattga gagctctttc ttgattctgt gggtggtggt 1200 gcatggccgt tcttagttgg tgggttgcct tgtcaggttg attccggtaa cgaacgagac 1260 ctcagcctgc taaatagtca gcatcgcacc tgcggtgcgc ggacttctta gagggactat 1320 tggcgttcag ccaatggaag tatgaggcga taacaggtct gtgatgccct tagatgttct 1380 gggccgcacg cgcgctacac tgacgcgacc aacgagccta tccttggccg agaggcccgg 1440 gtaatcttgt aaaccgcgtc gtgatgggga tagattattg caattattag tcttcaacga 1500 ggaatgccta gtaagcgcga gtcatcagct cgcgttgatt acgtccctgc cctttgtaca 1560 caccgcccgt cgctcctacc gattgggtgt gctggtgaag tgttcggatt ggtcttgact 1620 ggggcaacct ggtcttggct gaaaag 1646 <210> 5 <211> 1601 <212> DNA <213> Unknown <220> <223> KSF0003 <400> 5 ttatttgatg gtactttact cggataaccg tagtaattct agagctaata cgtgcgtaaa 60 tcccgacttc tggaagggac gtatttatta gataaaaggc cagccgggct tgcccgacct 120 gaggcgaatc atgataactt cacgaatcgc acggccttgt gccggcgatg tttcattcaa 180 atttctgccc tatcaacttt cgatggtagg atagaggcct accatggtgg taacgggtga 240 cggaggatta gggttcgatt ccggagaggg agcctgagaa acggctacca catccaagga 300 aggcagcagg cgcgcaaatt acccaatccc gacacgggga ggtagtgaca ataaataaca 360 atactgggca tttatgtctg gtaattggaa tgagtacaat gtaaatatct taacgagtat 420 ccattggagg gcaagtctgg tgccagcagc cgcggtaatt ccagctccaa tagcgtatat 480 ttaagttgtt gcagttaaaa agctcgtagt tggatttcgg gtgtggcggc gcggtctggt 540 tctactatgt actgcgttcg gcacaccttt ctgctgggga cgggctcctg ggcttcactg 600 tctgggactc ggaatcagcg aagtgacctt gagcaaacaa gagtgttcaa agcaagccta 660 cgctctgaat tttttagcat ggaatcacac gataggactc tggcctatct tgttggtctg 720 taggaccgga gtaatgatta agagggacag tcgggggcat tcgtatttca ttgtcagagg 780 tgaaattctt ggatttatga aagacgaact tctgcgaaag catttgccaa ggatgttttc 840 attgatcaag aacgaaagtt gggggctcga agacgattag ataccgtcgt agtctcaacc 900 ataaacgatg ccgactaggg attggcaggt gttccattga tgaccctgcc agcaccttat 960 gagaaatcaa agtttttggg ttccgggggg agtatggtcg caaggctgaa acttaaagga 1020 attgacggaa gggcaccacc aggcgtggag cctgcggctt aatttgactc aacacgggga 1080 aacttaccag gtccagacac ggggaggatt gacagattga gagctctttc ttgattctgt 1140 gggtggtggt gcatggccgt tcttagttgg tgggttgcct tgtcaggttg attccggtaa 1200 cgaacgagac ctcagcctgc taaatagtca cgggtacctt ggtacacgct tgacttctta 1260 gagggactat tggcgtttag tcaatggaag tgtgaggcaa taacaggtct gtgatgccct 1320 tagatgttct gggccgcacg cgcgctacac tgatgcattc aacgagccta tccttggccg 1380 agaggtccgg gtaatcttcg aaactgcatc gtgatgggga tagattattg caattattag 1440 tcttcaacga ggaatgccta gtaagcgcaa gtcatcagct tgcgttgatt acgtccctgc 1500 cctttgtaca caccgcccgt cgctcctacc gattgggtgt gctggtgaag agttcggatt 1560 ggccttgacg gggggaaact tctgtcttgg ctgagaagaa c 1601 <210> 6 <211> 1652 <212> DNA <213> Unknown <220> <223> KSF0134 <400> 6 actgctttat actgtgaaac tgcgaatggc tcattaaatc agttatagtt tatttgatgg 60 tacctactac tcggataccc gtagtaattc tagagctaat acgtgcgtaa atcccgactt 120 ctggaaggga cgtatttatt agataaaagg ccagccgggc tctgcccgac ctgcggtgaa 180 tcatgataac ttcacgaatc gtatggcctc gtgccgacga tgtttcattc aaatttctgc 240 cctatcaact ttcgatggta ggatagaggc ctaccatggt ggtaacgggt gacggaggat 300 tagggttcga ttccggagag ggagcctgag agatggctac cacatccaag gaaggcagca 360 ggcgcgcaaa ttacccaatc ccaacacggg gaggtagtga caataaataa caataccggg 420 catttaatgt ctggtaattg gaatgagtac aatctaaatc ccttaacgag gatccattgg 480 agggcaagtc tggtgccagc agccgcggta attccagctc caatagcgta tatttaagtt 540 gttgcagtta aaaagctcgt agttggattt cgggtgggtt cgagtcggtc cgcctctggt 600 gtgcactgct tgggcctacc tttctgtcgg ggacgggctc ctggggttaa ctcttcggga 660 ctcggagtcg acgatgttac tttgagtaaa ttagagtgtt caaagcaagc ctacgctctg 720 aatacattag catggaataa cgcgatagga ctctggccta tcttgttggt ctgtaggact 780 ggagtaatga ttaagaggga cagtcggggg cattcgtatt acattgtcag aggtgaaatt 840 cttggattta tgtaagacga acttctgcga aagcatttgc caaggatgtc ttcattaatc 900 aagaacgaaa gttgggggct cgaagacgat tagataccgt cgtagtctca accataaacg 960 atgccgacta gggattggcg ggtgtttcgt tgatgacccc gccagcacct tatgagaaat 1020 caaagtcttt gggttccggg gggagtatgg tcgcaaggct gaaacttaaa ggaattgacg 1080 gaagggcacc accaggcgtg gagcctgcgg cttaatttga ctcaacacgg gaaaacttac 1140 caggtccaga cacgggaagg attgacagat tgagagctct ttcttgattc tgtgggtggt 1200 ggtgcatggc cgttcttagt tggtgggttg ccttgtcagg ttgattccgg taacgaacga 1260 gacctcagcc tgctaaatag ccacagttgc ctctggcagc tggccggctt cttagaggga 1320 ctattgtcgt ttaggcaatg gaagtatgag gcaataacag gtctgtgatg cccttagatg 1380 ttctgggccg cacgcgcgct acactgatgc attcaacgag cctatccttg gccgagaggc 1440 ccgggtaatc tttgaaactg catcgtgatg gggatagatt attgcaatta ttagtcttca 1500 acgaggaatg cctagtaagc gcgagtcatc agctcgcgtt gattacgtcc ctgccctttg 1560 tacacaccgc ccgtcgctcc taccgattgg gtgtgctggt gaagtgttcg gattggtttc 1620 agttggtggc aacatcgatt gagactgaaa ag 1652 <110> Gachon University of Industry-Academic Cooperation Foundation Korea Institute of Ocean Science & Technology <120> COMPOSITION HAVING RADICAL SCAVENGING ACTIVITY CONTAINING AN EXTRACT OF POLAR MICROALGAE AS AN ACTIVE INGREDIENT <130> P22-E022 <160> 6 <170> KoPatentIn 3.0 <210> 1 <211> 1636 <212> DNA <213> unknown <220> <223> KSF0041 <400> 1 actgcgaatg gctcattaaa tcagttatag tttattgat ggtacctact actcggatac 60 ccgtagtaaa tctagagcta atacgtgcgt aaatcccgac ttctggaagg gacgtattta 120 ttagataaaa ggccgaccgg gctctgcccg actcgcggtg aatcatgata acttcacgaa 180 tcgcatggcc ttgtgccggc gatgtttcat tcaaatttct gccctatcaa ctttcgatgg 240 taggatagag gcctaccatg gtggtaacgg gtgacggagg attagggttc gattccggag 300 agggagcctg agaaacggct accacatcca agggaaggcag caggcgcgca aattacccaa 360 tcctgacaca gggaggtagt gacaataaat aacaatactg ggccttttca ggtctggtaa 420 ttggaatgag tacaatctaa accccttaac gaggatcaat tggagggcaa gtctggtgcc 480 agcagccgcg gtaattccag ctccaatagc gtatatttaa gttgctgcag ttaaaaagct 540 cgtagttgga tttcgggtgg ggcctgccgg tccgccgttt cggtgtgcac tggcagggcc 600 caccttgttg ccggggacgg gctcctgggc ttcactgtcc gggactcgga gtcggcgctg 660 ttactttgag taaattagag tgttcaaagc aggcctacgc tctgaataca ttagcatgga 720 ataacacgat aggactctgg cctatcctgt tggtctgtag gaccggagta atgattaaga 780 ggggacagtcg ggggcattcg tatttcattg tcagaggtga aattcttgga tttatgaaag 840 acgaactact gcgaaagcat ttgccaagga tgttttcatt aatcaagaac gaaagttggg 900 ggctcgaaga cgattagata ccgtcctagt ctcaaccata aacgatgccg actagggatc 960 ggcggatgtt tcttcgatga ctccgccggc accttatgag aaatcaaagt ttttgggttc 1020 cggggggagt atggtcgcaa ggctgaaact taaaggaatt gacggaaggg caccaccagg 1080 cgtggagcct gcggcttaat ttgactcaac acgggaaaac ttaccaggtc cagacatagt 1140 gaggattgac agattgagag ctctttcttg attctatggg tggtggtgca tggccgttct 1200 tagttggtgg gttgccttgt caggttgatt ccggtaacga acgagacctc agcctgctaa 1260 atagtcacga ttggctcgcc agtcggcgga cttcttagag ggactattgg cgactagcca 1320 atggaagcat gaggcaataa caggtctggg atgcccttag atgttctggg ccgcacgcgc 1380 gctacactga tgcattcaac gagcttagcc ttggccgaga ggcccgggta atctttgaaa 1440 ctgcatcgtg atggggatag attattgcaa ttattaatct tcaacgagga atgcctagta 1500 agcgcaagtc atcagcttgc gttgattacg tccctgccct ttgtacacac cgcccgtcgc 1560 tcctaccgat tgggtgtgct ggtgaagtgt tcggattggc gactgggggc ggtctccgct 1620 ctcagccgcc gaaaag 1636 <210> 2 <211> 1705 <212> DNA <213> unknown <220> <223> KNM0029C <400> 2 gcgaatggct cattaaatca gttatagttt atttgatggt acctctactc ggataaccgt 60 agtaattcta gagctaatac gtgcgcaaat cccgacttct ggaagggacg tatttattag 120 ataaaaggcc agccgagctt gctcgacctg cggtgactca taataacttc acgaatcgca 180 cggccttgcg ccggcgatgt ttcattcaaa tttctgccct atcaactttc gatggtagga 240 tagaggccta ccatggtggt aacgggtgac ggaggattag ggttcgattc cggagaggga 300 gcctgagaaa cggctaccac atccaaggaa ggcagcaggc gcgcaaatta cccaatcccg 360 acacggggag gtagtgacaa taaataacaa taccgggcgt tcaacgtctg gtaattggaa 420 tgagtacaat ttaaatccct taacgaggat ccattggagg gcaagtctgg tgccagcagc 480 cgcggtaatt ccagctccaa tagcgtatat ttaagttgct gcagttaaaa agctcgtagt 540 tggatttcgg gcgagtgacg acggtccgcc tctggtgtgc actgccgttc acccgccttt 600 ctgtcgggaa cgtgctcctg gcctttactg tctgggacac gcgttcgacg aggttacttt 660 gagtaaatta gagtgttcaa agcaggcatc gccttgaata tattagcatg gaatagcacg 720 ataggactct ggcctatttt gctggtctgt aggacccggag taatgattaa gagggacagt 780 cgggggcatt cgtatttcat tgtcagaggt gaaattcttg gatttatgaa agacgaactt 840 ctgcgaaagc atttgccaag gatgttttca ttgatcaaga acgaaagttg ggggctcgaa 900 gacgattaga taccgtcgta gtctcaacca taaacgatgc cgaccaggga ttaggcagtg 960 ttcttttgat gacctgccta gcaccttgag agaaatcaga gtttttgggt tccgggggga 1020 gtatggtcgc aaggctgaaa cttaaaggaa ttgacggaag ggcaccacca ggcgtggagc 1080 ctgcggctta atttgactca acacgggaaa acttaccagg tccagacacg gggatgataa 1140 acagattgag agctcttttt tgattctgtg ggtggtggtg catggccgtt cttagttggt 1200 gggttgcctt gtcaggttga ttccggtaac gaacgagacc tcagcctgct aaatagtcac 1260 atatcctttt tggatatgcc tgacttctta gagggactat tgtcgtttag gcaatggaag 1320 tgtgaggcaa taacaggtct gtgatgccct tagatgttct gggccgcacg cgcgctacac 1380 tgatgcattc aacgagccta tccttggcag aaatgcccgg gtaatctttg aaactgcatc 1440 gtgatgggga tagattattg caattattag tcttcaacga ggaatgccta gtaagcgcga 1500 gtcatcagct cgcgctgatt acgtccctgc cctttgtaca caccgcccgt cgctcctacc 1560 gattgggtgt gctggtgaga actgaggatt ggctgttatt ggtggcaaca ctgattatgg 1620 ccgagaacca gtttaaaccc tcccacctag agggaaggaga agtcgtaaca aggtttccgt 1680 aggtgaacct gcggaaggat cattg 1705 <210> 3 <211> 1609 <212> DNA <213> unknown <220> <223> KSF0015 <400> 3 ctgcgaatgg ctcattaaat cagttatagt ttatttgatg gtacctacta ctcggatacc 60 cgtagtaaat ctagagctaa tacgtgcgta aatcccgact tctggaaggg acgtatttat 120 tagataaaag gccgaccggg ctctgcccga ctcgcggtga atcatgataa cttcacgaat 180 cgcatggcct tgtgccggcg atgtttcatt caaatttctg ccctatcaac tttcgatggt 240 aggatagagg cctaccatgg tggtaacggg tgacggagga ttagggttcg attccggaga 300 gggagcctga gaaacggcta ccacatccaa ggaaggcagc aggcgcgcaa attacccaat 360 cctgacacag ggaggtagtg acaataaata acaatactgg gccttttcag gtctggtaat 420 tggaatgagt acaatctaaa ccccttaacg aggatcaatt ggagggcaag tctggtgcca 480 gcagccgcgg taattccagc tccaatagcg tatatttaag ttgctgcagt taaaaagctc 540 gtagttggat ttcgggtggg gcctgccggt ccgccgtttc ggtgtgcact ggcagggccc 600 accttgttgc cggggacggg ctcctgggct tcactgtccg ggactcggag tcggcgctgt 660 tactttgagt aaattagagt gttcaaagca ggcctacgct ctgaatacat tagcatggaa 720 taacacgata ggactctggc ctatcctgtt ggtctgtagg accggagtaa tgattaagag 780 ggacagtcgg gggcattcgt atttcattgt cagaggtgaa attcttggat ttatgaaaga 840 cgaactactg cgaaagcatt tgccaaggat gttttcatta atcaagaacg aaagttgggg 900 gctcgaagac gattagatac cgtcctagtc tcaaccataa acgatgccga ctagggatcg 960 gcggatgttt cttcgatgac tccgccggca ccttatgaga aatcaaagtt tttgggttcc 1020 ggggggagta tggtcgcaag gctgaaactt aaaggaattg acggaagggc accaccaggc 1080 gtggagcctg cggcttaatt tgactcaaca cgggaaaact taccaggtcc agacatagtg 1140 aggattgaca gattgagagc tctttcttga ttctatgggt ggtggtgcat ggccgttctt 1200 agttggtggg ttgccttgtc aggttgattc cggtaacgaa cgagacctca gcctgctaaa 1260 tagtcacgat tggctcgcca gtcggcggac ttcttagagg gactattggc gactagccaa 1320 tggaagcatg aggcaataac aggtctgtga tgcccttaga tgttctgggc cgcacgcgcg 1380 ctacactgat gcattcaacg agcttagcct tggccgagag gcccgggtaa tctttgaaac 1440 tgcatcgtga tggggataga ttattgcaat tattaatctt caacgaggaa tgcctagtaa 1500 gcgcaagtca tcagcttgcg ttgattacgt ccctgccctt tgtacacacc gcccgtcgct 1560 cctaccgatt gggtgtgctg gtgaagtgtt cggattggcg actgggggc 1609 <210> 4 <211> 1646 <212> DNA <213> unknown <220> <223> KSF0037 <400> 4 actgcttata ctgtgaaact gcgaatggct cattaaatca gttatagttt atttgatggt 60 acctactact cggataaccg tagtaattct agagctaata cgtgcgcaca acccgacttc 120 tggaagggtc gtattttatta gataaaaggc cagccgggct ctgcccgacc tgcggtgaat 180 catgataact tcacgaatcg tatggcctcg cgccgacgat gtttcattca aatttctgcc 240 ctatcaactt tcgatggtag gatagaggcc taccatggtg gtaacgggtg acggaggatt 300 agggttcgat tccggagagg gagcctgaga gatggctacc acatccaagg aaggcagcag 360 gcgcgcaaat tacccaatcc cgacacgggg aggtagtgac aataaataac aataccgggc 420 gcttcgcgtc tggtaattgg aatgagtaca atctaaatcc cttaacgagg atccattgga 480 gggcaagtct ggtgccagca gccgcggtaa ttccagctcc aatagcgtat atttaagttg 540 ttgcagttaa aaagctcgta gttggatttc gggtgggacg gtgcggtccg cctctggtgt 600 gcactgctct gttccacctt cctgccgggg acgggctcct gggcttcact gtctgggact 660 cggagtcggc gaggttactt tgagtaaatt agagtgttca aagcaggcct acgctctgaa 720 tacattagca tggaataaca cgataggact ctggcctatc tgttggtctg tgggaccgga 780 gtaatgatta agaggggtag tcggggacat tcgtattccg ttgtcagagg tgaaattctt 840 ggatttacgg aagacgaaca tctgcgaaag catttgtcaa ggatactttc attgatcaag 900 aacgaaagtt gggggctcga agacgattag ataccgtcgt agtctcaacc ataaacgatg 960 ccgactaggg attggcagat gttcttttga tgactctgcc agcaccttat gagaaatcaa 1020 agtttttggg ttccgggggg agtatggtcg caaggctgaa acttaaagga attgacggaa 1080 gggcaccacc aggcgtggag cctgcggctt aatttgactc aacacgggga aacttaccag 1140 gtccagacac gggaaggatt gacagattga gagctctttc ttgattctgt gggtggtggt 1200 gcatggccgt tcttagttgg tgggttgcct tgtcaggttg attccggtaa cgaacgagac 1260 ctcagcctgc taaatagtca gcatcgcacc tgcggtgcgc ggacttctta gagggactat 1320 tggcgttcag ccaatggaag tatgaggcga taacaggtct gtgatgccct tagatgttct 1380 gggccgcacg cgcgctacac tgacgcgacc aacgagccta tccttggccg agaggcccgg 1440 gtaatcttgt aaaccgcgtc gtgatgggga tagattattg caattattag tcttcaacga 1500 ggaatgccta gtaagcgcga gtcatcagct cgcgttgatt acgtccctgc cctttgtaca 1560 caccgcccgt cgctcctacc gattgggtgt gctggtgaag tgttcggatt ggtcttgact 1620 ggggcaacct ggtcttggct gaaaag 1646 <210> 5 <211> 1601 <212> DNA <213> unknown <220> <223> KSF0003 <400> 5 ttatttgatg gtacttact cggataaccg tagtaattct agagctaata cgtgcgtaaa 60 tcccgacttc tggaagggac gtattttatta gataaaaggc cagccgggct tgcccgacct 120 gaggcgaatc atgataactt cacgaatcgc acggccttgt gccggcgatg tttcattcaa 180 atttctgccc tatcaacttt cgatggtagg atagaggcct accatggtgg taacgggtga 240 cggaggatta gggttcgatt ccggagaggg agcctgagaa acggctacca catccaagga 300 aggcagcagg cgcgcaaatt acccaatccc gacacgggga ggtagtgaca ataaataaca 360 atactgggca tttatgtctg gtaattggaa tgagtacaat gtaaatatct taacgagtat 420 ccattggagg gcaagtctgg tgccagcagc cgcggtaatt ccagctccaa tagcgtatat 480 ttaagttgtt gcagttaaaa agctcgtagt tggatttcgg gtgtggcggc gcggtctggt 540 tctactatgt actgcgttcg gcacaccttt ctgctgggga cgggctcctg ggcttcactg 600 tctgggactc ggaatcagcg aagtgacctt gagcaaacaa gagtgttcaa agcaagccta 660 cgctctgaat tttttagcat ggaatcacac gataggactc tggcctatct tgttggtctg 720 taggaccgga gtaatgatta agaggggacag tcgggggcat tcgtatttca ttgtcagagg 780 tgaaattctt ggatttatga aagacgaact tctgcgaaag catttgccaa ggatgttttc 840 attgatcaag aacgaaagtt gggggctcga agacgattag ataccgtcgt agtctcaacc 900 ataaacgatg ccgactaggg attggcaggt gttccattga tgaccctgcc agcaccttat 960 gagaaatcaa agtttttggg ttccgggggg agtatggtcg caaggctgaa acttaaagga 1020 attgacggaa gggcaccacc aggcgtggag cctgcggctt aatttgactc aacacgggga 1080 aacttaccag gtccagacac ggggaggatt gacagattga gagctctttc ttgattctgt 1140 gggtggtggt gcatggccgt tcttagttgg tgggttgcct tgtcaggttg attccggtaa 1200 cgaacgagac ctcagcctgc taaatagtca cgggtacctt ggtacacgct tgacttctta 1260 gagggactat tggcgtttag tcaatggaag tgtgaggcaa taacaggtct gtgatgccct 1320 tagatgttct gggccgcacg cgcgctacac tgatgcattc aacgagccta tccttggccg 1380 agaggtccgg gtaatcttcg aaactgcatc gtgatgggga tagattattg caattattag 1440 tcttcaacga ggaatgccta gtaagcgcaa gtcatcagct tgcgttgatt acgtccctgc 1500 cctttgtaca caccgcccgt cgctcctacc gattgggtgt gctggtgaag agttcggatt 1560 ggccttgacg gggggaaact tctgtcttgg ctgagaagaa c 1601 <210> 6 <211> 1652 <212> DNA <213> unknown <220> <223> KSF0134 <400> 6 actgctttat actgtgaaac tgcgaatggc tcattaaatc agttatagtt tatttgatgg 60 tacctactac tcggataccc gtagtaattc tagagctaat acgtgcgtaa atcccgactt 120 ctggaaggga cgtatttatt agataaaagg ccagccgggc tctgcccgac ctgcggtgaa 180 tcatgataac ttcacgaatc gtatggcctc gtgccgacga tgtttcattc aaatttctgc 240 cctatcaact ttcgatggta ggatagaggc ctaccatggt ggtaacgggt gacggaggat 300 tagggttcga ttccggagag ggagcctgag agatggctac cacatccaag gaaggcagca 360 ggcgcgcaaa ttacccaatc ccaacacggg gaggtagtga caataaataa caataccggg 420 catttaatgt ctggtaattg gaatgagtac aatctaaatc ccttaacgag gatccattgg 480 agggcaagtc tggtgccagc agccgcggta attccagctc caatagcgta tatttaagtt 540 gttgcagtta aaaagctcgt agttggattt cgggtgggtt cgagtcggtc cgcctctggt 600 gtgcactgct tgggcctacc tttctgtcgg ggacgggctc ctggggttaa ctcttcggga 660 ctcggagtcg acgatgttac tttgagtaaa ttagagtgtt caaagcaagc ctacgctctg 720 aatacattag catggaataa cgcgatagga ctctggccta tcttgttggt ctgtaggact 780 ggagtaatga ttaagaggga cagtcggggg cattcgtatt acattgtcag aggtgaaatt 840 cttggattta tgtaagacga acttctgcga aagcatttgc caaggatgtc ttcattaatc 900 aagaacgaaa gttgggggct cgaagacgat tagataccgt cgtagtctca accataaacg 960 atgccgacta gggattggcg ggtgtttcgt tgatgacccc gccagcacct tatgagaaat 1020 caaagtcttt gggttccggg gggagtatgg tcgcaaggct gaaacttaaa ggaattgacg 1080 gaagggcacc accaggcgtg gagcctgcgg cttaatttga ctcaacacgg gaaaacttac 1140 caggtccaga cacgggaagg attgacagat tgagagctct ttcttgattc tgtgggtggt 1200 ggtgcatggc cgttcttagt tggtgggttg ccttgtcagg ttgattccgg taacgaacga 1260 gacctcagcc tgctaaatag ccacagttgc ctctggcagc tggccggctt cttagaggga 1320 ctattgtcgt ttaggcaatg gaagtatgag gcaataacag gtctgtgatg cccttagatg 1380 ttctgggccg cacgcgcgct acactgatgc attcaacgag cctatccttg gccgagaggc 1440 ccgggtaatc tttgaaactg catcgtgatg gggatagatt attgcaatta ttagtcttca 1500 acgaggaatg cctagtaagc gcgagtcatc agctcgcgtt gattacgtcc ctgccctttg 1560 tacacaccgc ccgtcgctcc taccgattgg gtgtgctggt gaagtgttcg gattggtttc 1620 agttggtggc aacatcgatt gagactgaaa ag 1652
Claims (3)
상기 라디칼 소거 활성은 H2O2 라디칼 소거 활성 또는 OH 라디칼 소거 활성인 것을 특징으로 하는 라디칼 소거 활성을 갖는 조성물.According to claim 1,
The radical scavenging activity is a composition having a radical scavenging activity, characterized in that the H 2 O 2 radical scavenging activity or OH radical scavenging activity.
상기 OH 라디칼은 DNA를 손상시키는 것을 특징으로 하는 라디칼 소거 활성을 갖는 조성물.According to claim 2,
The OH radical is a composition having a radical scavenging activity, characterized in that DNA damage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020220024393A KR20230127412A (en) | 2022-02-24 | 2022-02-24 | Composition having radical scavenging activity containing an extract of polar microalgae as an active ingredient |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020220024393A KR20230127412A (en) | 2022-02-24 | 2022-02-24 | Composition having radical scavenging activity containing an extract of polar microalgae as an active ingredient |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20230127412A true KR20230127412A (en) | 2023-09-01 |
Family
ID=87975039
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020220024393A KR20230127412A (en) | 2022-02-24 | 2022-02-24 | Composition having radical scavenging activity containing an extract of polar microalgae as an active ingredient |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR20230127412A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102054391B1 (en) | 2018-06-28 | 2019-12-11 | 한국해양과학기술원 | Composition for Preventing or Treating Inflammation or Cancer Containing Extract of Micractinium sp. |
-
2022
- 2022-02-24 KR KR1020220024393A patent/KR20230127412A/en not_active Application Discontinuation
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102054391B1 (en) | 2018-06-28 | 2019-12-11 | 한국해양과학기술원 | Composition for Preventing or Treating Inflammation or Cancer Containing Extract of Micractinium sp. |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP3808836A1 (en) | Yeast for producing tyrosol and hydroxytyrosol and construction method | |
| Vallejo et al. | Cell aggregations in yeasts and their applications | |
| CN112384611B (en) | Lactobacillus crispatus KBL693 strain and application thereof | |
| WO2003008606A3 (en) | Process for the preparation of l-amino acids using strains of the enterobacteriaceae family which contain an enhanced phob or phor gene | |
| CN109609526B (en) | Barley stripe disease pathogenic gene PgPBS and application thereof | |
| Straver et al. | Cause and control of flocculation in yeast | |
| Barbosa et al. | Susceptibility to oral antiseptics and virulence factors ex vivo associated with Candida spp. isolated from dental prostheses | |
| KR20170143053A (en) | Cosmetic composition for improving anti-oxidation, anti-wrinkle and moisturizing comprising fermented product of used water from washing rice and preparation method of the same | |
| US20080242856A1 (en) | Extracts of taiwanofungus camphoratus with a capacity for inhibiting the activity of matrix metalloproteinases and method for preparing the same | |
| CN106471121A (en) | Reduce the Horizontal Gene Transfer of functional protein | |
| KR20230127412A (en) | Composition having radical scavenging activity containing an extract of polar microalgae as an active ingredient | |
| CN102517306A (en) | Ectoine synthetase gene, recombinant vector, recombinant engineering bacterium and application of recombinant engineering bacterium | |
| CN102994541B (en) | Method for enhancing secretion of glucose oxidase by coexpression of UPR (unfolded protein response) key genes and downstream target genes | |
| Tanaka et al. | Protoplasts of Pyricularia oryzae P2: preparation and regeneration into hyphal form | |
| KR20230127413A (en) | Composition for cell viability containing an extract of polar microalgae as an active ingredient | |
| KR20230127411A (en) | Composition for protecting a cell barrier containing an extract of polar microalgae as an active ingredient | |
| Motlhalamme et al. | Origin and Evolution of Yeasts | |
| CN111849984B (en) | IDH gene dsRNA capable of improving termite control effect of metarhizium anisopliae | |
| CN1294258C (en) | Method for separating antibiotic peptide and separated antibiotic peptide | |
| Brandhorst et al. | Using new genetic tools to study the pathogenesis of Blastomyces dermatitidis | |
| Kim et al. | SIMPLE METHOD FOR RNA PREPARATION FROM CYANOBACTERIA 1 | |
| CN112888712A (en) | Engineered microorganisms and methods of making and using the same | |
| Valdivieso‐Ugarte et al. | Expression of an Aspergillus niger Glucose Oxidase in Saccharomyces cerevisiae and Its Use to Optimize Fructo‐oligosaccharides Synthesis | |
| CN116478893B (en) | Construction and application of engineering probiotics for producing propionic acid | |
| Rai et al. | Genome sequencing and functional characterization of Xanthomonas cucurbitae, the causal agent of bacterial spot disease of cucurbits |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| E902 | Notification of reason for refusal |