KR20230120932A - Degradation method for cellulose acetate using Penicillium griseofulvum - Google Patents

Abstract

The present invention discloses a method for decomposing cellulose acetate using Penicillium griseofulvum , particularly Penicillium griseofulvum strain NIBRFGC000508019 (Accession Number: KACC 93376P).

Description

Degradation method for cellulose acetate using Penicillium griseofulvum {Degradation method for cellulose acetate using Penicillium griseofulvum}

The present invention relates to a method for decomposing cellulose acetate using Penicillium griseoful bum.

The word plastic comes from the Greek word "plastikos", which means "to be molded". It seems that the word came about because of the plastic's ability to be easily shaped when heated. Humanity has made ceaseless efforts to find materials that are beneficial to humans beyond materials that can be obtained from nature such as stone, metal, glass, and wood. It made possible plastics. Plastics are largely classified as “bioplastics” produced through chemical or biological processes using raw materials including renewable materials such as “non-degradable plastics (high-performance engineering plastics)” based on petrochemical materials and plant-derived resources (Biomass). It is divided into “Bioplastic”, and the recent high consumption trend for biodegradable, plant-based products and the institutional environment that encourages the use of bio-based products rather than petroleum-based products are further increasing the need for bioplastic development.

Cellulose is mainly used for bioplastics. Cellulose is a natural polymeric substance that exists in the largest amount on earth and is a major component of wood or cotton. are used in large quantities. In general, natural cellulose contains about 70% of crystal parts and has excellent mechanical properties. Therefore, molding is difficult (Polym (Korea). 2006;30(1):70-74).

Therefore, in order to compensate for these disadvantages, it is converted into a cellulose derivative and applied as a solution or melt processing method. Here, cellulose derivative refers to a product obtained by oxidation, substitution, and other chemical treatment of wood-based materials, and is used as a raw material for various chemical industries depending on the type of functional group to be substituted (Top Curr Chem. 2010;294:117-28). . However, plastics including cellulose derivatives, etc., whose disadvantages are compensated for in this way, are currently consumed worldwide at more than 2 million tons per year, showing an annual growth rate of about 5%. There is a lot of interest in possible or biodegradable resources. In particular, marine debris found in the ocean threatens the marine ecosystem, and various policies are being implemented to reduce the use of plastic.

In fact, cigarette filters in addition to plastic bags and plastic bottles were found to be threatening the marine ecosystem, and it was confirmed that cigarette filters accounted for the largest portion of the garbage collected from the beach over the past 30 years. Cellulose acetate (CA), one of the cellulose derivatives, is mainly used as a material for cigarette filters, and as a result of research on the decomposition of cigarette filters, the main component of which is CA, in the natural state, soil landfill or surface Corrosion of the cigarette filter was observed upon exposure, but it was confirmed that the filter shape was maintained as it was. Also, it is known from data announced by the Ministry of Environment that it takes about 10 to 12 years for the cigarette filter to completely decompose when exposed to the ground. .

In foreign countries, bacteria such as Neisseria sicca , Rhizobium meliloti , and Alcaligenes xylosoxidans and Pestalothiopsis westerdijkii QM 381, The possibility of decomposing CA using enzymes secreted by microorganisms such as yeast has been reported, but there is no research related to this in Korea.

The present invention discloses the CA resolution of Penicillium griseofulvum strains isolated and identified in domestic soil.

An object of the present invention is to provide a method for decomposing cellulose acetate using a strain of Penicillium griseofull.

Other objects or specific objects of the present invention will be presented below.

As confirmed in the following examples and experimental examples, the present invention isolates and identifies a fungus, Penicillium griseofulvum strain, from domestic soil, and the isolated and identified strain is cellulose acetate. It was completed by confirming that it has the resolution of (Cellulose acetate).

Considering the foregoing, it can be understood that the decomposition method of cellulose acetate of the present invention includes the step of culturing Penicillium griseofullum in contact with cellulose acetate.

In the method of the present invention, cellulose acetate means any acetate ester of cellulose polymer represented by Formula 1 below, and preferably means a cellulose diacetate polymer.

<Formula 1>

In addition, in the method of the present invention, in order to increase the decomposition of cellulose acetate by Penicillium griseofulvum by promoting the culture and growth of Penicillium griseofulvum, the culture medium obtained by culturing Penicillium griseofulvum in a medium A medium for promoting the culture and growth of Penicillium griseofulbum before or after contact with the raw cellulose acetate film or before and after contact with the cellulose acetate film may be added to and mixed with the cellulose acetate film.

Such media generally contain a carbon source, a nitrogen source, trace elements, growth promoters, and the like.

The carbon source is preferably a sugar such as a monosaccharide, disaccharide or polysaccharide. For example, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch, starch hydrolyzate and the like can be used. Complex compounds, such as molasses or by-products of sugar refining processes, may also be used. In some cases, oils such as soybean oil and sunflower oil, organic acids such as acetic acid, and glycerol may be preferred as the carbon source. These carbon sources may be included in the medium alone or in a mixture of two or more, and whether included in the medium alone or in a mixture of two or more, 2% (w / w) to 40% (w / w) It can be included in the medium as a range. When natural products or processed products of natural products, such as starch or starch hydrolyzate, are used as carbon sources, these carbon sources may be sterilized to prevent unwanted fermentation by other microorganisms. Sterilization may be performed using methods known in the art, such as high-temperature and high-pressure sterilization and ultraviolet irradiation.

As the nitrogen source, an inorganic nitrogen compound, an organic nitrogen compound, or a complex compound containing these compounds may be used. Examples of inorganic nitrogen compounds include ammonia, ammonium salts (eg, ammonium sulfate, ammonium chloride, ammonium phosphate, ammonium carbonate, ammonium nitrate, etc.), nitrate, and the like, and examples of organic nitrogen compounds include urea, amino acids, and the like. Complex compounds include yeast extract, soytone, peptone, tryptone, malt extract, broth, soybean powder, soybean okara powder, cheonggukjang powder, soybean paste Natural nitrogen sources, such as powder, etc. are mentioned. One or more of these nitrogen sources may also be used in combination, and may be included in the medium in the range of 0.1% (w/w) to 8.0% (w/w). When using a natural nitrogen source, it is preferable to sterilize and use it for the same reason as described in relation to the case of using a natural carbon source.

Trace elements include calcium, magnesium, sodium, cobalt, molybdenum, potassium, manganese, zinc, copper, iron, phosphorus, sulfur, etc. These trace elements may be added to the medium in the form of salt compounds, and the medium of these trace elements A chelating agent such as catechol, citric acid, or the like may be added together to increase solubility in and maintain a solution state. Examples of the salt compound form include sodium hydrogen phosphate, magnesium sulfate, iron chloride, calcium salt, manganese salt, cobalt salt, molybthenate salt, chelate metal salt and the like. These trace elements may be used in combination of one or more, and may be included in the medium in the range of 0.001% (w/w) to 3.0% (w/w).

Biotin, riboflavin, thiamin, folic acid, nicotinic acid, pantothenate, pyridoxine, etc. may be used as growth promoters, and may be included in the medium in the range of 0.0001% (w/w) to 1.0% (w/w).

The medium may be prepared by mixing the above-described carbon source, nitrogen source, trace element, growth promoter, etc., but a medium of known composition commonly used in the culture of fungi in the art, such as BHI liquid medium (Brain-heart Infusion Broth Medium), Czapek Brotho Medium, PDB Medium (Potato Dextrose Broth Medium), SD Medium (Sabouraud Dextrose Medium), SM Medium (Sabouraud Maltose Medium) may also be used. There is much literature in the art on optimization of medium composition, including Applied Microbiol. Physiology, A Practical Approach (Editors P.M. Rhodes, P.F. Stanbury, IRL Press (1997) pp. 53-73, ISBN 0 19 963577 3). It is known, and reference can be made to these known documents.

The medium is preferably used after being sterilized to prevent the proliferation of unnecessary microorganisms. The sterilization method of the medium is known in the art, and a suitable method may be selected and used among known sterilization methods. For example, high-pressure steam sterilization, a sterilization method using ultraviolet rays, and the like may be suitable.

The culture temperature is generally 15°C to 45°C, preferably 25°C to 40°C, more preferably 25°C to 30°C. The pH of the medium is 4 to 8.5, preferably 4.0 to 7.0. The pH of the medium can be adjusted by adding a basic compound such as sodium hydroxide, potassium hydroxide, aqueous ammonia, or an acidic compound such as phosphoric acid or sulfuric acid while the culture is in progress.

Antifoaming agents such as fatty acid polyglycol esters may be added to remove foam formed during cultivation.

Cultivation may be advantageous to create aerobic conditions or anaerobic conditions depending on the growth characteristics.

Cultivation can be batchwise, semi-batchwise or continuous. Suitable specific culture methods are described, for example, in Bioprozeβtechnik 1. Einfuhrung in die Bioverfahrenstechnik [Bioprocess technology 1. Introduction to Bioprocess technology] (Gustav Fischer Verlag, Stuttgart, 1991), Bioreaktoren und periphere Einrichtungen [Bioreactors and peripheral equipment] (Vieweg Verlag, Brunswick/Wiesbaden, 1994), the American Society for Bacteriology (Manual of Methods for General Bacteriology" (Washington D.C., USA, 1981), etc. may be referred to.

In another aspect, the present invention relates to the Penicillium griseofulvum NIBRFGC000508019 strain (Accession Number: KACC 93376P).

The Penicillium griseofulbum NIBRFGC000508019 strain (accession number: KACC 93376P) according to the present invention has an internal transcribed spacer (ITS) gene, beta-tubulin (BTU) gene, calmodulin (CaM) gene, RNA polymerase II subunit (RPB2) It can be understood as a strain whose genes have sequences of SEQ ID NOs: 9, 10, 11, and 12, respectively.

As described above, according to the present invention, it is possible to provide a method for decomposing cellulose acetate using Penicillium griseofulbum.

The method of the present invention can be usefully applied to the decomposition of cellulose acetate, which is difficult to decompose in nature.

Figure 1 is a photograph of the culture of Penicillium griseopulbum NIBRFGC000508019 strain in PDA medium for 7 days.
Figure 2 is a phylogenetic tree of Penicillium griseofullbum NIBRFGC000508019 strain.
Figure 3 is a reaction scheme showing the decomposition mechanism of cellulose acetate.
Figure 4 is the result of confirming the cellulose acetate decomposition ability of the Penicillium griseopulbum NIBRFGC000508019 strain using X-ray diffraction analysis.
Figure 5 is the result of confirming the cellulose acetate decomposition ability of the Penicillium griseofullbum NIBRFGC000508019 strain using infrared spectroscopy.

Hereinafter, the present invention will be described with reference to examples. However, the scope of the present invention is not limited to these examples.

<Example 1> Separation of strain Penicillium griseofulbum NIBRFGC000508019

For the isolation of Penicillium griseofulvum NIBRFGC000508019 strain, 10 mL of sterile water was added to 1 g of a soil sample collected from Mt. Trash, Uiseong-gun, Gyeongsangbuk-do, Korea, and shaken at 150 rpm for 30 minutes in a shaking incubator. Thereafter, the shaken suspension was diluted 10-fold in sterile water to make a diluted solution, and 100 μL of the diluted solution was spread on potato dextrose agar (PDA, Difco, USA) and cultured in the dark at 25 ° C. Mycelia observed after 1-2 days of culture were subcultured to a new PDA, designated as NIBRFGC000508019, and used as a test strain.

The results of culturing the strain NIBRFGC000508019 for 7 days in PDA medium are shown in Figure 1 (A in Figure 1 is a front picture, B in Figure 1 is a picture of the back side, C in Figure 1 is a picture after 40 days of culturing the strain in cellulose ethyl acetate) ), the center of the cells was slightly raised, the cells were white-green and mixed, and the edges were white. The back has a light brown edge and the center has a dark brown color.

<Example 2> Identification of Penicillium griseoful bum NIBRFGC000508019 strain

For the molecular biological identification of the strain Penicillium griseofulvum NIBRFGC000508019, the genomic DNA of the strain was extracted using HiGeneTM Genomic DNA prep kit (BIOFACT, Daejeon, Korea), and the ITS region for sequencing analysis , Beta-tubulin (BTU), Calmodulin (CaM), and RNA polymerase II subunit (RPB 2) genes were subjected to PCR and sequencing.

For ITS region gene amplification, forward/reverse primers of SEQ ID NOs: 1 and 2 (ITS1 (5'-TCC GTA GGT GAA CCT GCG G-3'), ITS4 (5'-TCC TCC GCT TAT TGA TAT GC-3')) For BTU gene amplification, forward/reverse primers of SEQ ID NOs: 3 and 4 (Bt2a (5'-GGT AAC CAA ATC GGT GCT GCT TTC-3'), Bt2b (5'-ACC CTC AGT GTA GTG ACC CTT GGC-3') )), forward/reverse primers of SEQ ID NOs: 5 and 6 (CL1 (5'-GAR TWC AAG GAG GCC TTC TC-3'), CL2A (5'-TTT TTG CAT CAT GAG TTG GAC-3') for CaM gene amplification )), forward/reverse primers of SEQ ID NOs: 7 and 8 (5F2 (5'-GGG GWG AYC AGA AGA AGG C-3'), 7cR (5'-CCC ATR GCT TGY TTR CCC AT-3') for RPB2 gene amplification )) was used to perform PCR, and the amplified product was purified using EXOSAP-IT (Thermo Fisher Scientific, Waltham, MA, USA). The purified PCR product was sent to Bioneer Co., Ltd. (Daejeon, Korea) for sequencing, and the received data was sequenced using SeqMan Lasergene software (DNAStar Inc., Madison, Wisconsin, USA). It was obtained (SEQ ID NO: 9 for the ITS region gene, SEQ ID NO: 10 for the BTU gene, SEQ ID NO: 11 for the CaM gene, and SEQ ID NO: 12 for the RPB2 gene).

The National Center for Biotechnology Information's Blast program (Basic Local Alignment Search Tool) was used to compare sequence homology with previously reported strains. The phylogenetic relationship was created using MEGA7.0 software, and the phylogenetic tree created is shown in FIG.

As a result of blasting the isolated strain at NCBI, the BTU gene showed 100% homology with the strain Penicillium griseofulvum NRRL 2300, and the CaM gene showed Penicillium griseofulvum DTO 072A5. Shows 99.8% homology with the strain, and the RPB2 gene shows 99.8% homology with Penicillium griseofulvum DTO 072A5 strain and 100% homology with Dierchx 1901 strain. This was confirmed, and it was identified as Penicillium griseofulvum by forming the same group on the phylogenetic tree with Penicillium griseofulvum NRRL 2300 strain, and it was identified as Penicillium griseofulvum , and the Korean Agricultural Culture Collection , KACC) and assigned accession number KACC 93376P.

<Example 3> Confirmation of resolution of CA using X-ray diffraction analysis and infrared spectroscopy

Cellulose Acetate (Film) (Sigma-aldrich GF68004772, thickness 0.025mm) was used as the CA film used in the experiment, and in order to remove contaminants on the surface, it was wiped 2-3 times with 70% EtOH, and 5× It was cut into 6 cm and used as an experimental material.

The culture medium was prepared as follows using the method of Mao et al. (Mao et al., 2015). Minimum medium without carbon source (MgSO4 7H ₂ O 0.5 g/L, NH ₄ Cl 1.0 g/L, KH ₂ PO ₄ 5.54 g/L, Na ₂ HPO ₄ 4.78 g/L, CaCl ₂ 7H ₂ O 0.005 g / L, Agar powder 15 g / L) was prepared and put on the prepared CA film, and the test strain was plated on the surface of the film and cultured in a 25 ℃ incubator.

As shown in FIG. 3, the CA film can undergo decomposition of the main chain and the acetyl group (R=COCH3) through oxidation and hydrolysis. In order to confirm the effect of the strain on the CA film, after 40 days of cultivation, the CA film (C in FIG. 1) was analyzed by X-ray diffraction (XRD) and infrared spectrophotometer (IR). Using this, the chemical structure and crystal structure changes of the CA film before and after culturing the strain were analyzed.

The results of confirming the CA resolution through XRD analysis are shown in FIG. 4, and in the process of decomposition of the polymer, decomposition occurs first in the amorphous region, and as the decomposition progresses, the crystal structure changes. In addition, when an acetyl group is decomposed and a hydroxyl group is generated, the crystal structure is affected due to an increase in hydrogen bonding. In the case of a general CA film, diffraction peaks appear at 2θ = 11.7 ^o , 20.1 ^o , and 21.7 ^o . As a result of XRD analysis, it was confirmed that the diffraction pattern of the CA film appeared differently before and after culturing the strain. Through this, it was confirmed that there was a change in the microstructure of the CA film, and it seems to be because the decomposition of the CA molecular chain affects the crystal structure disorder and I _β crystal form due to the acetyl group of the film.

The results of confirming the CA resolution through IR analysis are shown in FIG. 5. In the case of the IR spectrum, if the type of functional group is different, the band area cannot be directly compared, but a qualitative comparison can be made from the relative size of the two bands. In FIG. 5, the CA film has a carbonyl group (C=O) of an acetyl group at 1750 cm ^-1 , a broad hydroxyl group (-OH) at 3470 cm ^-1 , a methyl group (CH3) at 1370 cm ^-1 , and a COC of a Pyranoid ring at 1040 cm ^-1 A band of functional groups appears. Since the acetyl group is converted to a hydroxyl group as the CA film is hydrolyzed, the C=O and CH bands of the acetyl group decrease and the -OH band gradually increases compared to before culturing the strain. Through this, when the ratio of each functional group of the samples after mold culture was calculated, it can be seen that the acetyl group was decomposed in the CA film after strain culture.

<110> National Institute of Biological Resources <120> Degradation method for cellulose acetate using Penicillium griseofulvum <130> P21-037 <160> 12 <170> KoPatentIn 3.0 <210> 1 <211> 19 <212> DNA <213> artificial sequence <220> <223> Forward primer of beta-tubulin <400> 1 tccgtaggtg aacctgcgg 19 <210> 2 <211> 20 <212> DNA <213> artificial sequence <220> <223> Forward primer of beta-tubulin <400> 2 tcctccgctt attgatatgc 20 <210> 3 <211> 24 <212> DNA <213> artificial sequence <220> <223> Forward primer of beta-tubulin <400> 3 ggtaaccaaa tcggtgctgc tttc 24 <210> 4 <211> 916 <212> DNA <213> artificial sequence <220> <223> Backward primer of beta-tubulin <400> 4 tggtatgtcc caggtgctga gtcgttacac atttgcctct tcattgtctc atctgcgccg 60 cacgaacacc cccattggca gagatggaaa gattgccaaa ccccgtcaac tccataatac 120 tcactggggt ctggtctgcc cggctgagac tcccgaaggt caagcttgtg gtctggtcaa 180 gaacttggca ttgatgtgct acatcactgt cggtacacct gcagagccta tcgtggactt 240 tatgattcag cgcaacatgg aagtcctcga ggagttcgaa ccccaagtga cgcccaatgc 300 gacaaaggtg tttgttaatg gtgtttgggt gggtattcac cgggaccctt cgcatctcgt 360 tactacgatg cagaacctgc gtcgacgaaa catgatctca cacgaagtca gtttgattcg 420 tgacatccgt gaacgggaat tcaaaatctt cacggatact ggacgtgtct gccggccact 480 cttcgtcatc gataatgatc ccaagagtga aaactcgggt ggattggtcc ttaataagga 540 acacattcgg aagctcgagg ccgacaaaga cttgccagta gacttggccc cagaagaacg 600 tcgcgaacaa tacttcggat gggatggtct ggtgcgttct ggagcagtcg agtatgtcga 660 tgccgaagaa gaggaaacca tcatgattgt catgaccccc gaggatctcg agatctctcg 720 acagcttcag gccggttacg ctctgccaga tgacgaagcc ggcgacccca ataaacgtgt 780 tcgatcgatt ctcagccagc gtgcccacac ctggacgcac tgtgaaatcc accctagtat 840 gatcttgggt gtttgcgcta gtattattcc tttcccggat cacaaccagt cgccccgtaa 900 cacctaccag tctgcc 916 <210> 5 <211> 20 <212> DNA <213> artificial sequence <220> <223> Forward primer of Calmodulin <400> 5 gartwcaagg aggccttctc 20 <210> 6 <211> 21 <212> DNA <213> artificial sequence <220> <223> Backward primer of Calmodulin <400> 6 tttttgcatc atgagttgga c 21 <210> 7 <211> 19 <212> DNA <213> artificial sequence <220> <223> Forward primer of RNA polymerase II subunit <400> 7 ggggwgayca gaagaaggc 19 <210> 8 <211> 20 <212> DNA <213> artificial sequence <220> <223> Backward primer of RNA polymerase II subunit <400> 8 cccatrgctt gyttrcccat 20 <210> 9 <211> 552 <212> DNA <213> artificial sequence <220> <223> ITS region <400> 9 cctgcggaag gatcattact gagtgagggc cctctgggtc caacctccca cccgtgttta 60 tttaccttgt tgcttcggcg ggcccgcctt aactggccgc cggggggctt acgcccccgg 120 gcccgcgccc gccgaagaca ccctcgaact ctgtctgaag attgtagtct gagtgaaaat 180 ataaattatt taaaactttc aacaacggat ctcttggttc cggcatcgat gaagaacgca 240 gcgaaatgcg atacgtaatg tgaattgcaa attcagtgaa tcatcgagtc tttgaacgca 300 cattgcgccc cctggtattc cggggggcat gcctgtccga gcgtcattgc tgccctcaag 360 cacggcttgt gtgttgggcc ccgtcctccg attccggggg acgggcccga aaggcagcgg 420 cggcaccgcg tccggtcctc gagcgtatgg ggctttgtca cccgctctgt aggcccggcc 480 ggcgcttgcc gatcaaccca aatttttatc caggttgacc tcggatcagg tagggatacc 540 cgctgaactt aa 552 <210> 10 <211> 432 <212> DNA <213> artificial sequence <220> <223> Beta-tubulin gene <400> 10 tggtacgtgc ctagcttttt ttttcccgcg ttgggtatca attgacaagt tactaactgg 60 attacaggca aaccatttcc ggcgagcacg gtctcgatgg tgatggacag taagttaatg 120 gcgacgaatt ctccagtgga tggcatgtct gatatcttgt taggtacaat ggtacctccg 180 acctccagct cgagcgtatg aacgtctact tcaaccatgt gagtatagcc gactgggaac 240 cgaataatcg tgcatcaact gatcgggtct ttttctttga tcatctaggc tagcggtgat 300 aagtacgttc cccgtgccgt tctggtcgat ttggagcccg gtaccatgga cgctgtccgc 360 tccggcccct tcggcaagct tttccgcccc gacaacttcg tcttcggtca gtccggtgct 420 ggtaacaact gg 432 <210> 11 <211> 664 <212> DNA <213> artificial sequence <220> <223> Calmodulin gene <400> 11 cctgtttgg agtgattcct acagattgaa gatattgaag atatgctgac cgggagtttg 60 ttttgcgaaa taggacaagg atggcgatgg tacgtgtggt tgcgcccgac agctcagttg 120 agcccacagc agcgtcctct gctttcgaat attaagagca aggtattcta acatacaatc 180 ctaccaatag gacaaatcac caccaaggag cttggtaccg tcatgcgctc gctgggccag 240 aacccctccg agtccgaact gcaggatatg atcaacgagg tcgacgccga caacaacggc 300 accattgact tccccggtac ttgctccata ccccactgat ataaataaga gacggttat 360 gacgtgcgat agaattcctg accatgatgg ctcgtaagat gaaggatact gattccgagg 420 aggagatccg tgaggcattc aaggttttcg atcgcgacaa caacggattc atttccgctg 480 ccgagctgcg ccacgtcatg acttcaatcg gtgagaagct gaccgacgac gaggttgacg 540 agatgatccg cgaggcggac caggatggtg atggccggat cgactgtatg ttttggaatc 600 ttggagccta ccggcacttg ccttcccgaa tgctaacctc cccatttata cagacaacga 660 gttc 664 <210> 12 <211> 916 <212> DNA <213> artificial sequence <220> <223> RNA polymerase II (RPB2) gene <400> 12 tggtatgtcc caggtgctga gtcgttacac atttgcctct tcattgtctc atctgcgccg 60 cacgaacacc cccattggca gagatggaaa gattgccaaa ccccgtcaac tccataatac 120 tcactggggt ctggtctgcc cggctgagac tcccgaaggt caagcttgtg gtctggtcaa 180 gaacttggca ttgatgtgct acatcactgt cggtacacct gcagagccta tcgtggactt 240 tatgattcag cgcaacatgg aagtcctcga ggagttcgaa ccccaagtga cgcccaatgc 300 gacaaaggtg tttgttaatg gtgtttgggt gggtattcac cgggaccctt cgcatctcgt 360 tactacgatg cagaacctgc gtcgacgaaa catgatctca cacgaagtca gtttgattcg 420 tgacatccgt gaacgggaat tcaaaatctt cacggatact ggacgtgtct gccggccact 480 cttcgtcatc gataatgatc ccaagagtga aaactcgggt ggattggtcc ttaataagga 540 acacattcgg aagctcgagg ccgacaaaga cttgccagta gacttggccc cagaagaacg 600 tcgcgaacaa tacttcggat gggatggtct ggtgcgttct ggagcagtcg agtatgtcga 660 tgccgaagaa gaggaaacca tcatgattgt catgaccccc gaggatctcg agatctctcg 720 acagcttcag gccggttacg ctctgccaga tgacgaagcc ggcgacccca ataaacgtgt 780 tcgatcgatt ctcagccagc gtgcccacac ctggacgcac tgtgaaatcc accctagtat 840 gatcttgggt gtttgcgcta gtattattcc tttcccggat cacaaccagt cgccccgtaa 900 cacctaccag tctgcc 916

Claims (5)

A method of decomposing cellulose acetate comprising the step of contacting and culturing cellulose acetate with Penicillium griseofulvum .
According to claim 1,
The Penicillium griseofulbum has an internal transcribed spacer (ITS) gene, a beta-tubulin (BTU) gene, a calmodulin (CaM) gene, and an RNA polymerase II subunit (RPB2) gene of SEQ ID NOs: 9, 10, 11, and 12, respectively. A method characterized in that it is a strain having a sequence.
According to claim 1,
The contact of the Penicillium griseofulbum with the cellulose acetate is made by contacting the cellulose acetate in the form of a culture medium obtained by culturing the Penicillium griseofullbum in a medium,
The method characterized in that the medium contains at least one of a carbon source, a nitrogen source, a trace element and a growth promoter.
According to claim 1,
Before and after the contact of the cellulose acetate and Penicillium griseofulbum, a medium for promoting the culture and growth of Penicillium griseofullbum is added to the cellulose acetate and mixed,
The method characterized in that the medium contains at least one of a carbon source, a nitrogen source, a trace element and a growth promoter.
Penicillium griseofulbum NIBRFGC000508019 strain (accession number: KACC 93376P).