KR20230119989A - Cosmetic composition comprising multi-layered lamellar nano liposomes containing high concentration of ceramide and method for preparing the same - Google Patents

Abstract

It relates to a cosmetic composition comprising multi-lamellar nanoliposomes containing high concentration of ceramide and a method for preparing the same. The cosmetic composition exhibits a temperature stability improvement effect, an emulsion stability improvement effect, a solubilization stability improvement effect, a dispersion stability improvement effect, or a skin permeability improvement effect while containing ceramide at a high concentration.

Description

Cosmetic composition comprising multi-layered lamellar nano liposomes containing high concentration of ceramide and method for preparing the same}

It relates to a cosmetic composition comprising multi-lamellar nanoliposomes containing high concentration of ceramide and a method for preparing the same.

Lipids between keratinocytes, which play an important role in the skin permeation barrier, are composed of ceramide, cholesterol, fatty acids, and neutral lipids, and it is known that changes in these major components affect the skin barrier function. Among them, ceramide is a major lipid constituting the lamellar structure of lipids between keratinocytes, and is well known to play an essential role in the function as a skin permeability barrier. It is known that the amount of these ceramides decreases with skin aging, and accordingly, the skin barrier function decreases, resulting in a decrease in the amount of moisture in the skin, an increase in the loss of moisture, a peeling phenomenon of the stratum corneum, and the like, resulting in damaged or unhealthy skin barriers. These symptoms can be improved by supplying ceramide to the skin, but ceramide is a poorly soluble substance and has a problem in that it is difficult to contain a large amount in cosmetics due to its structural specificity.

When ceramide is contained in a large amount in a cosmetic formulation, when ceramide particles are prepared and used in a small size, the stability in the formulation decreases, and a phenomenon in which crystallization and precipitation causes gelation (gelation) occurs. Korean Patent Registration No. 10-2325570 proposes a stabilization method by preparing nanoliposomes containing oxygenated water and ceramides, but there is a limitation in containing high concentrations of ceramides. Therefore, there is still a need for a technique capable of stabilizing a cosmetic composition containing a high content of ceramide by overcoming the limitations of the prior art.

Under this background, the present inventors developed a cosmetic composition comprising a multi-layered lamellar nanoliposome containing a high concentration of ceramide, and by confirming remarkably excellent temperature stability, emulsification, solubilization, or dispersion stability, and skin permeability of the cosmetic composition , completed the present invention.

One aspect is to provide a cosmetic composition comprising a multi-lamellar nanoliposome containing a high concentration of ceramide.

Another aspect is to provide a method for preparing the cosmetic composition.

One aspect provides a cosmetic composition comprising a ceramide, an amphoteric surfactant, a nonionic surfactant, and an anionic surfactant.

The ceramide is a type of sphingolipid having a structure in which a fatty acid is linked to sphingosine or phytosphingosine. The ceramide may include both natural ceramide and pseudo ceramide. Natural ceramides are ceramides that exist in nature, and may be ceramides extracted from animals, plants, and microorganisms. Pseudoceramides may refer to compounds having a double-chain lamellar structure of natural ceramides. The ceramide may be at least one selected from the group consisting of ceramide EOP, ceramide NS, ceramide NP, ceramide AS, ceramide EOS, ceramide NDS, ceramide AP, glucosylceramide, and omega hydroxy ceramide, but is not limited thereto. In addition, the ceramide may be at least one selected from the group consisting of ceramide 1, ceramide 2, ceramide 3, ceramide 4, ceramide 5, ceramide 6, ceramide 7, ceramide 8, and ceramide 9, but is not limited thereto.

The cosmetic composition may include liposome particles containing the ceramide, the amphoteric surfactant, the nonionic surfactant, and the anionic surfactant. In other words, the ceramide, the amphoteric surfactant, the nonionic surfactant, and the anionic surfactant may be present in the cosmetic composition in a state in which liposome particles are formed.

The liposome is defined as a spherical phospholipid vesicle of colloidal particles that associate themselves. Liposomes composed of amphiphilic molecules having a water-soluble head (hydrophilic group) and an insoluble tail (hydrophobic group) have an ordered structure that is spontaneously associated by their interaction. It is classified into SUV (Small Unilamellar Vesicle), LUV (Large Unilamellar Vesicle), and MLV (Multi Lamellar Vesicle) according to their size and lamellarity. Liposomes exhibiting various stacking properties have a double membrane structure similar to that of cell membranes. Therefore, in the liposome particles included in the cosmetic composition, the amphoteric surfactant, the nonionic surfactant, and the anionic surfactant form an outer membrane (eg, a double membrane), and the ceramide is enclosed therein. It may have a form that has been made.

The liposome particles included in the cosmetic composition may be multi-lamellar or multi-layered lamellar liposome particles. More specifically, the liposome particles included in the cosmetic composition may be multi-layer lamellar nano-liposome particles. In other words, the liposome particles included in the cosmetic composition may have a multilayer lamellar structured membrane and a core for collecting the ceramide, and the multilayered lamellar structured membrane includes the amphoteric surfactant and the nonionic surfactant. , And it may be made of the anionic surfactant. Therefore, the multilayer lamellar liposome particles included in the cosmetic composition exist in a state in which ceramide is trapped therein, contributing to stabilization of ceramide, and stably emulsifying, solubilizing, or dispersing poorly soluble ceramide in the aqueous phase. .

The term "lamella" means a double membrane structure made by two unit films and may mean having a polarizing Maltese-Cross structure.

Also, the term “multi-layered lamellar” may refer to a structure in which the lamellar structure is composed of two or more layers.

The term “encapsulation” means to surround and encapsulate a target material.

According to one embodiment, the liposome particles (specifically, multi-layered lamellar liposome particles) included in the cosmetic composition are about 50 to 500, 50 to 300, 50 to 200, 50 to 150, 100 to 500, 100 to It may be nanoliposome particles having an average particle diameter of 300, 100 to 200, 100 to 150, 200 to 500, 200 to 300, 300 to 500, or 300 to 400 nm. Therefore, according to one embodiment, the liposome particles included in the cosmetic composition have a uniform nano size, so that they can be stably dispersed in the aqueous phase and can be easily penetrated into the skin. For this reason, the cosmetic composition may exhibit remarkably excellent temperature stability, emulsion stability, solubilization stability, or dispersion stability, while containing ceramide at a high concentration, and may exhibit remarkably excellent skin permeability.

The cosmetic composition may contain the ceramide at a high concentration by forming the liposome particles (specifically, multi-lamellar liposome particles or multi-lamellar nanoliposome particles). Specifically, the cosmetic composition may contain about 0.001 to 30, 1 to 30, or 10 to 30% by weight of the ceramide.

The cosmetic composition may contain about 1 to 10, 3 to 7, or 5% by weight of the amphoteric surfactant.

The amphoteric surfactant is lauramidopropyl betaine, cocamidopropyl betaine, lauryl hydroxysultaine, coco-betaine, lauryl betaine, disodium cocoamphodiacetate, sodium cocoamphodiacetate, lauramido It may contain at least one selected from the group consisting of propyl hydroxysultaine, cocamidopropyl hydroxysultaine, sodium sweet almond amphoacetate, sodium lauroamphoacetate, babasuamidopropyl betaine, and lecithin, It is not limited to this. Most preferably, the amphoteric surfactant may be lecithin.

The term “lecithin” refers broadly to a group of yellowish-brown fatty substances occurring in animal and plant tissues composed of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides, and phospholipids. Lecithin may be a mixture of diglycerides of stearic acid, palmitic acid, and oleic acid linked to the choline ester of phosphoric acid. The lecithin can be obtained from various sources such as eggs and soybeans.

The lecithin may be at least one selected from the group consisting of soybean lecithin, egg lecithin, and hydrogenated lecithin. The lecithin may be hydrogenated lecithin. The hydrogenated lecithin may be the product of controlled hydrogenation of lecithin.

The cosmetic composition may contain about 12 to 24 or 15 to 20% by weight of the nonionic surfactant. According to one embodiment, when the cosmetic composition contains the nonionic surfactant in an amount outside the above numerical range, the liposome particles in the cosmetic composition (specifically, multi-lamellar liposome particles or multi-lamellar nanoliposomes) particles) may not be stably formed, and as a result, temperature stability, emulsification, solubilization, or dispersion stability, or skin permeability of the cosmetic composition may be significantly reduced.

The nonionic surfactant may include, but is not limited to, polyglycerin fatty acid ester, glyceryl citrate/lactate/linoleate/oleate, or a combination thereof.

The polyglycerin fatty acid ester is polyglyceryl-2 caprate, polyglyceryl-3 caprate, polyglyceryl-4 caprate, polyglyceryl-4 caprate, Polyglyceryl-2 oleate, polyglyceryl-4 oleate, polyglyceryl-5 oleate, polyglyceryl-6 oleate 6 oleate), polyglyceryl-2 sesquioleate, polyglyceryl-2 laurate, polyglyceryl-10 laurate, polyglyceryl Polyglyceryl-10 myristate, polyglyceryl-10 oleate, polyglyceryl-10 palmitate, polyglyceryl-10 stearate 10 stearate), polyglyceryl-10 isostearate, polyglyceryl-10 diisostearate, and polyglyceryl-10 distearate It may include one or more selected from the group consisting of, but is not limited thereto. Most preferably, the polyglycerin fatty acid ester may be polyglyceryl-10 stearate, polyglyceryl-10 laurate, or a combination thereof.

According to one embodiment, the cosmetic composition may include the polyglycerin fatty acid ester and the glyceryl citrate/lactate/linoleate/oleate as nonionic surfactants. In this case, according to one embodiment, the cosmetic composition contains the polyglycerin fatty acid ester and the glyceryl citrate/lactate/linoleate/oleate in a ratio of about 4 to 11: 9 to 11, 1: 1.5 to 2.5, 1 : It may be included in a weight ratio of 0.9 to 1.1, 1: 2, or 1: 1. According to one embodiment, when the cosmetic composition includes the polyglycerin fatty acid ester and the glyceryl citrate / lactate / linoleate / oleate in a weight ratio outside the numerical range, the liposome particles in the cosmetic composition ( Specifically, multi-layer lamellar liposome particles or multi-layer lamellar nano-liposome particles) may not be stably formed, and as a result, the temperature stability, emulsification, solubilization, or dispersion stability, or skin permeability of the cosmetic composition is significantly reduced. may be lowered

According to one embodiment, the cosmetic composition, as a nonionic surfactant, the polyglyceryl-10 stearate, the polyglyceryl-10 laurate, and the glyceryl citrate / lactate / linoleate / oleate It may contain. In this case, according to one embodiment, the cosmetic composition comprises the polyglyceryl-10 stearate, the polyglyceryl-10 laurate, and the glyceryl citrate/lactate/linoleate/oleate in a ratio of about 1: It may be included in a weight ratio of 1: 1.5 to 5, 1: 1: 1.5 to 3, or 1: 1: 2. According to one embodiment, the cosmetic composition comprises the polyglyceryl-10 stearate, the polyglyceryl-10 laurate, and the glyceryl citrate / lactate / linoleate / oleate in a weight ratio outside the above numerical range When included as, the liposome particles (specifically, multi-lamellar liposome particles or multi-lamellar nanoliposome particles) may not be stably formed in the cosmetic composition, and thus, the temperature stability of the cosmetic composition, or Emulsification, solubilization, or dispersion stability, or skin permeability may be significantly lowered.

The cosmetic composition may contain about 0.1 to 5, 0.5 to 2, or 1% by weight of the anionic surfactant.

The anionic surfactant is ammonium lauryl sulfate, ammonium laureth sulfate, sodium laureth sulfate, sodium lauryl sulfate, sodium dilauramidoglutamide lysine, sodium lauryl phosphate, disodium lauryl phosphate, potassium laureth At least one selected from the group consisting of phosphate, potassium lauryl phosphate, lauryl phosphate, glutamic acid, aspartic acid, DEA-lauryl sulfate, DEA-laureth sulfate, and sodium lauroyl glutamate It may include, but is not limited thereto. Most preferably, the anionic surfactant may be sodium lauroyl glutamate.

In one embodiment, the cosmetic composition, about 0.001 to 30, 1 to 30, or 10 to 30% by weight of the ceramide; about 1 to 10, 3 to 7, or 5% by weight of the amphoteric surfactant (specifically, lecithin); About 12 to 24 or 15 to 20% by weight of the nonionic surfactant (specifically, polyglycerin fatty acid ester and glyceryl citrate/lactate/linoleate/oleate); And about 0.1 to 5, 0.5 to 2, or 1% by weight of the anionic surfactant (specifically, sodium lauroyl glutamate), the polyglycerin fatty acid ester and the glyceryl citrate / lactate / linoleum The weight ratio of ate/oleate may be about 4 to 11:9 to 11, 1:1.5 to 2.5, 1:0.9 to 1.1, 1:2, or 1:1. According to one embodiment, the ceramide contained in the cosmetic composition, the amphoteric surfactant, the nonionic surfactant, and the weight percent of the anionic surfactant or the polyglycerin fatty acid ester and the glyceryl citrate / lactate If the weight ratio of / linoleate / oleate is out of the above numerical range, the liposome particles (specifically, multi-lamellar liposome particles or multi-lamellar nanoliposome particles) may not be stably formed in the cosmetic composition, , As a result, temperature stability, or emulsification, solubilization, or dispersion stability, or skin permeability of the cosmetic composition may be significantly reduced.

According to one embodiment, the cosmetic composition forms the liposome particles (specifically, the multi-lamellar liposome particles or the multi-lamellar nanoliposome particles) in which the ceramide is collected, while containing ceramide at a high concentration and significantly excellent temperature stability, emulsion stability, solubilization stability, or dispersion stability. Through this, it is possible to stably emulsify, solubilize, or disperse high-concentration ceramide in the aqueous phase.

In addition, according to one embodiment, due to the nano size of the liposome particles (specifically, the multi-lamellar liposome particles or the multi-lamellar nanoliposome particles) in which the ceramide is collected in the cosmetic composition, the cosmetic The composition can exhibit remarkably good skin permeability.

The cosmetic composition may have a zeta potential value of -60 mV to -30 mV.

The term "zeta potential" means the magnitude of the repulsive force and attractive force between particles in units. represents density. Particles of colloidal matter in water have the property of floating rather than settling. These materials have the property of repelling each other due to the + and - ions of the particles, and this repulsive force can be called zeta potential. Therefore, the zeta potential can be applied to evaluate the degree of dispersion or aggregation of particles suspended in a liquid phase, and generally, the higher the absolute value of the zeta potential, the more stable the dispersion of the particles in the suspension can be evaluated.

According to one embodiment, since the absolute value of the zeta potential of the cosmetic composition represents a high value of about 30 to 60 mV, the liposome particles included in the cosmetic composition are very stably dispersed in the cosmetic composition, and the liposome It was found that the particles form a more stable liposome structure by forming strong static interactions, for example, ionic bonds, between the internal negative charge and the external positive charge.

The cosmetic composition may further include a glycol compound.

The glycol compounds include ethylene glycol, diethylene glycol, propylene glycol, dipropylene glycol, butylene glycol, pentylene glycol, hexylene glycol, and ethoxydiglycol. It may be one or more selected from the group consisting of, but is not limited thereto.

The cosmetic composition may contain about 10 to 40 or 20 to 30% by weight of the glycol compound.

The cosmetic composition may be a cosmetic raw material composition used for manufacturing cosmetics. In addition, the cosmetic composition may be a cosmetic composition having a final cosmetic formulation.

The cosmetic composition may further include components such as surfactants, antioxidants, preservatives, pH adjusters, moisturizers, lubricants, emulsifiers, solubilizers, and solvents, if necessary. In addition, the cosmetic composition may further include a material that can supplementally provide essential nutrients to the skin or a material that improves skin conditions, such as a functional material having an effect such as improving wrinkles or whitening. The cosmetic composition may contain adjuvants including, but not limited to, natural fragrances, cosmetic fragrances, or plant extracts.

The cosmetic composition may be prepared in any formulation conventionally prepared in the art. The cosmetic composition includes, for example, solutions, emulsions, suspensions, pastes, creams, lotions, gels, powders, sprays, surfactant-containing cleansing agents, oils, soaps, liquid cleansers, bath additives, foundations, makeup bases, essences, It can be prepared in formulations such as lotion, foam, pack, softening water, sunscreen cream, sun oil, etc., and preferably external skin ointment, softening lotion, nutrient lotion, nutrient cream, massage cream, essence, pack, emulsion, or oil It can be prepared in the form of a gel. The carrier of the cosmetic composition may be selectively used depending on the formulation of the raw product or cosmetic. For example, when preparing an ointment, paste, cream, or gel-type raw product or cosmetic, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, Zinc oxide and the like can be used alone or in combination. In the case of manufacturing raw materials or cosmetics in the form of powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, chlorofluorohydrocarbon, propane/butane, dimethyl ether, etc. may be used alone or in combination. When preparing a raw material product or cosmetic in the form of a solution or emulsion, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, cottonseed as a carrier component oil, peanut oil, corn germ oil, olive oil, castor oil, sesame oil, glycerol aliphatic ester, polyethylene glycol, or sorbitan fatty acid ester, etc. may be used alone or in combination. When preparing a raw material product or cosmetic in the form of a suspension, water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum meta as a carrier component Hydroxide, bentonite, agar, tracanth and the like may be used alone or in combination. In the case of manufacturing a raw material product or cosmetics in the form of cosmetic soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, Sugars and the like can be used alone or in combination.

Another aspect provides a method for preparing a cosmetic composition comprising mixing purified water, ceramide, an amphoteric surfactant, a nonionic surfactant, and an anionic surfactant.

In the above method, the mixing may further include adding and mixing a glycol compound.

In the above method, the specific type, mixed weight %, or mixed weight ratio of each component is as mentioned in the description of the cosmetic composition.

In the method, specific conditions and methods for manufacturing may be based on conventional techniques in the art, but are not limited thereto.

Among the terms or elements mentioned in the manufacturing method, the same as those mentioned in the description of the composition are understood to be the same as those mentioned in the description of the composition above.

The cosmetic composition containing ceramide, amphoteric surfactant, nonionic surfactant, and anionic surfactant according to one aspect forms a multi-layered lamellar nanoliposome, thereby containing ceramide at a high concentration while having remarkably excellent temperature stability, emulsion stability, solubilization stability, or dispersion stability. Through this, it is possible to stably disperse a high concentration of ceramide in the aqueous phase. In addition, the composition may exhibit remarkably excellent skin permeability due to the nano size of the liposome particles.

1 is a diagram showing the results of microscopic imaging of multi-layer lamellar nanoliposome particles containing ceramide included in the cosmetic composition of Example 2 of one aspect.
Figure 2 is a diagram showing the results of analyzing the particle size of the cosmetic composition of Example 1 of one aspect.
Figure 3 is a diagram showing the results of analyzing the particle size of the cosmetic composition of Example 2 of one aspect.
Figure 4 is a diagram showing the results of measuring the zeta potential of the cosmetic composition of Example 2 of one aspect.
Figure 5 is a diagram showing the results of confirming the particles included in the cosmetic composition of Example 1, Example 2, Comparative Example 5, and Comparative Example 6 of one aspect through microscopic imaging.
Figure 6 is a diagram showing the results of analyzing the skin permeability of the cosmetic compositions of Example 1, Example 2, Comparative Example 5, and Comparative Example 6 in one aspect.

Hereinafter, the present invention will be described in more detail through experimental examples and examples. However, these experimental examples and examples are intended to illustrate the present invention by way of example, and the scope of the present invention is not limited to these experimental examples and examples.

In addition, unless there is a specific definition in the present specification, all scientific and technical terms used herein may have the same meaning as commonly understood by a person skilled in the art to which the present invention belongs.

Example 1. - Example 2. Preparation of a cosmetic composition containing multi-layered lamellar nanoliposomes containing ceramide

In this example, a cosmetic composition containing multi-lamellar nanoliposomes containing ceramide was prepared, and the results are shown in Table 1 below.

Specifically, phases A to D of Table 1 were prepared by weighing each component included in each phase of Table 1 below by the amount shown in Table 1 below. Then, phase A was added to phase B, heated to about 70° C., and then stirred for about 10 minutes at a speed of about 2500 rpm with a homogenizer to obtain a mixture of phases A and B. Thereafter, phase C was added to the mixture of phases A and B, stirred at a rate of about 2500 rpm with a homogenizer at about 70 ° C. for about 10 minutes, and then cooled to about 45 ° C. A mixture of phase A, phase B, and phase C was obtained. Thereafter, phase D was added to the mixture of phases A, B, and C, and stirred for about 3 minutes at a speed of about 2500 rpm with a homogenizer, and then at room temperature (about 10 to 30 ° C.) After cooling, a cosmetic composition comprising multi-lamellar nanoliposomes containing ceramide was obtained (Examples 1 and 2). In addition, compared to the examples, nonionic surfactants (specifically, polyglyceryl-10 stearate (Polyglyceryl-10 Stearate), polyglyceryl-10 laurate (Polyglyceryl-10 Laurate), or glyceryl citrate / A cosmetic composition was prepared with different contents of lactate/linoleate/oleate (Glyceryl Citrate/Lactate/Linoleate/Oleate) and the contents of other raw materials maintained the same as in Examples, and used as a comparative example ( Comparative Examples 1 to 6). In addition, microscopy was performed on the prepared cosmetic composition to confirm whether multi-layered lamellar nanoliposome particles containing ceramide were formed.

No award
(Phase) ingredient
(Unit: % by weight) Example
One Example 2 comparative example
One comparative example
2 comparative example
3 comparative example
4 Comparative Example 5 Comparative Example 6 One A Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 2 B Ceramide NP 20 20 20 20 20 20 20 20 3 Amphoteric surfactant: Hydrogenated Lecithin 5 5 5 5 5 5 5 5 4 Non-ionic surfactant: Polyglyceryl-10 Stearate 5 5 One - 15 - 5 5 5 Non-ionic surfactants:
Polyglyceryl-10 Laurate - 5 - One - 15 5 5 6 Non-ionic surfactant: Glyceryl Citrate/Lactate/Linoleate/Oleate 10 10 10 10 10 10 5 30 7 Anionic Surfactant: Sodium Lauroyl Glutamate One One One One One One One One 8 Pentylene Glycol 10 10 10 10 10 10 10 10 9 Dipropylene Glycol 10 10 10 10 10 10 10 10 10 C Butylene Glycol 7 7 7 7 7 7 7 7 11 D 1,2-Hexanediol 2 2 2 2 2 2 2 2

As a result, as shown in Figure 1, in the case of the cosmetic composition of Examples 1 and 2 prepared above, it was confirmed that multi-layered lamellar nanoliposome particles containing ceramide were formed.

Experimental Example 1. Particle Size Analysis of Cosmetic Composition Containing Multi-Lamellar Nano Liposomes Containing Ceramide

In this experimental example, the particle size of Examples 1 to 2 and Comparative Examples 1 to 6 prepared above was analyzed using a particle size analyzer (Zetasizer nano ZS, MALVERN Co.).

As a result, as shown in Table 2, FIG. 2, and FIG. 3, in the case of Example 1, it has a uniform particle size (average particle diameter) of about 350 nm, and in the case of Example 2, about 139 nm It was confirmed that it had a uniform particle size (average particle diameter) of On the other hand, in the case of Comparative Examples 1 to 6, it was confirmed that they had a larger particle size (average particle diameter) (about 684 to 2340 nm) compared to Examples.

particle size Example 1 about 350 nm Example 2 about 139 nm Comparative Example 1 about 1650 nm Comparative Example 2 about 2340 nm Comparative Example 3 about 1251 nm Comparative Example 4 about 1315 nm Comparative Example 5 about 1213 nm Comparative Example 6 about 684 nm

Through this experimental example, in the case of the cosmetic composition of Example prepared above, together with ceramide, about 1 to 10% by weight of an amphoteric surfactant (specifically, lecithin); About 12 to 24% by weight of a nonionic surfactant (specifically, polyglycerin fatty acid ester and glyceryl citrate/lactate/linoleate/oleate); And an anionic surfactant (specifically, sodium lauroyl glutamate) about 0.1 to 5% by weight, polyglycerin fatty acid ester and glyceryl citrate / lactate / linoleate / oligo constituting the nonionic surfactant It was confirmed that ceramide-containing multilayer lamellar nanoliposomes having a uniform and very small size (specifically, about 50 to 500 nm) were formed by including 8 in a weight ratio of about 4 to 11:9 to 11. For this reason, it was found that the cosmetic composition of Example prepared above can exhibit excellent emulsification, solubilization, or dispersion stability while containing a high concentration of ceramide of about 20% by weight.

about 1 to 10% by weight of an amphoteric surfactant (specifically, lecithin), especially with ceramide; about 12 to 24% by weight of a nonionic surfactant (specifically, polyglycerin fatty acid ester and glyceryl citrate/lactate/linoleate/oleate); And an anionic surfactant (specifically, sodium lauroyl glutamate) about 0.1 to 5% by weight, polyglycerin fatty acid ester and glyceryl citrate / lactate / linoleate / oligo constituting the nonionic surfactant In the case of the cosmetic composition of Example 2 prepared above containing 8 in a weight ratio of about 1:0.9 to 1.1, ceramide-containing multilayer lamellar nanoliposomes having a uniform and smallest size (specifically, about 50 to 150 nm) It was confirmed that the formation of For this reason, it was found that the cosmetic composition of Example 2 prepared above can exhibit the best emulsification, solubilization, or dispersion stability while containing a high concentration of ceramide of about 20% by weight.

On the other hand, the amphoteric surfactant; nonionic surfactants; And a comparison in which the weight% of the anionic surfactant is out of the weight%, or the weight ratio of the polyglycerin fatty acid ester and glyceryl citrate / lactate / linoleate / oleate constituting the nonionic surfactant is out of the weight ratio In the case of the cosmetic composition of Example, it was confirmed that the particle size significantly increased (specifically, about 600 to 3000 nm). Through this, it was found that the cosmetic composition of Comparative Example did not form ceramide-containing multilayer lamellar nanoliposomes or was formed very unstable. Could know.

Experimental Example 2. Evaluation of stability of cosmetic composition containing multi-layered lamellar nano-liposomes containing ceramide

In this experimental example, in order to evaluate the stability of the cosmetic composition containing the multilayer lamellar nanoliposomes containing ceramide, the cosmetic compositions of Examples 1 to 2 and Comparative Example 6 in Table 1 were tested at 4 ° C, 25 ° C, and The cosmetic composition was stored for about 30 days at a temperature of 45° C. and the particle size (average particle diameter) of the cosmetic composition was analyzed.

As a result, as shown in Table 3 below, in the case of Examples 1 and 2, compared to Comparative Example 6, it was confirmed that the change in particle size was significantly less at all temperature conditions of 4 ° C, 25 ° C, and 45 ° C did In particular, in the case of Example 2, it was increased to a remarkably low level (about 1.06 to 1.19 times increased) at all temperature conditions of 4 ° C, 25 ° C, and 45 ° C, confirming that the change in particle size was the smallest at all temperature conditions. . On the other hand, in the case of Comparative Example 6, the increase in particle size was not large under the temperature condition of 4 ℃, but the particle size increased significantly (about 1.8 to 2.6 times increase) under the temperature conditions of 25 ℃ and 45 ℃ 25 ℃ and 45 ℃ It was confirmed that the change in particle size was very large under the temperature condition of ℃.

immediately after manufacture Store at 4℃
(after about 30 days) Storage at 25℃
(after about 30 days) Storage at 45℃
(after about 30 days) Example 1 about 308 nm about 364 nm about 394 nm about 455 nm Example 2 about 139 nm about 148 nm about 134 nm about 165 nm Comparative Example 6 about 894 nm about 945 nm about 1685 nm about 2315 nm

Through this experimental example, in the case of the cosmetic composition of Example prepared above, together with ceramide, about 1 to 10% by weight of an amphoteric surfactant (specifically, lecithin); about 12 to 24% by weight of a nonionic surfactant (specifically, polyglycerin fatty acid ester and glyceryl citrate/lactate/linoleate/oleate); And an anionic surfactant (specifically, sodium lauroyl glutamate) about 0.1 to 5% by weight, polyglycerin fatty acid ester and glyceryl citrate / lactate / linoleate / oligo constituting the nonionic surfactant A ceramide-containing multilayer having a uniform and very small size (specifically, about 50 to 500 nm) by including 8 in a weight ratio of about 4 to 11:9 to 11 (more specifically, about 1:0.9 to 1.1) Lamellar nanoliposomes are stably formed, and when stored for a long time at all temperature conditions of 4°C, 25°C, and 45°C, the particle size of the ceramide-containing multilayered lamellar nanoliposomes does not change significantly and is maintained at a constant level, resulting in excellent It was confirmed that it showed stability. On the other hand, the amphoteric surfactant; nonionic surfactants; And a comparison in which the weight% of the anionic surfactant is out of the weight%, or the weight ratio of the polyglycerin fatty acid ester and glyceryl citrate / lactate / linoleate / oleate constituting the nonionic surfactant is out of the weight ratio In the case of the cosmetic composition of the example, it has a relatively large size of particles (specifically, about 600 to 3000 nm), it was confirmed that the particle size further increases when stored for a long time at 25 ℃ and 45 ℃ temperature conditions. Through this, in the cosmetic composition of Comparative Example, ceramide-containing multi-layer lamellar nanoliposomes are not formed or are formed very unstable, so that the emulsification, solubilization, or dispersion stability of ceramide is significantly reduced when stored for a long time at 25 ° C and 45 ° C temperature conditions. It was found that the temperature stability was remarkably deteriorated.

Experimental Example 3. Zeta potential analysis of cosmetic composition containing multilayer lamellar nanoliposomes containing ceramide

In this experimental example, zeta potential was measured to evaluate the stability of the cosmetic composition including the multi-lamellar nanoliposome containing ceramide.

Specifically, the zeta potential was measured on the 30th day of storage for the cosmetic compositions of Examples 1 to 2 and Comparative Examples 5 to 6 in Table 1 using a particle size analyzer (Malvern).

As a result, as shown in Table 4 and FIG. 4 below, it was confirmed that the cosmetic compositions of Examples 1 and 2 had significantly higher zeta potential absolute values compared to the cosmetic compositions of Comparative Examples 5 and 6.

Zeta (mV) Example 1 -31.8 Example 2 -52.6 Comparative Example 5 -2.8 Comparative Example 6 -28.4

Through this experimental example, in the case of the cosmetic composition of Example prepared above, together with ceramide, about 1 to 10% by weight of an amphoteric surfactant (specifically, lecithin); about 12 to 24% by weight of a nonionic surfactant (specifically, polyglycerin fatty acid ester and glyceryl citrate/lactate/linoleate/oleate); And an anionic surfactant (specifically, sodium lauroyl glutamate) about 0.1 to 5% by weight, polyglycerin fatty acid ester and glyceryl citrate / lactate / linoleate / oligo constituting the nonionic surfactant By including 8 in a weight ratio of about 4 to 11: 9 to 11 or about 1: 0.9 to 1.1, it has a zeta potential value of about -55 to -30 mV, which is significantly higher than that of the cosmetic composition of Comparative Example. It was confirmed that it has an absolute value. Through this, in the cosmetic composition of Example prepared above, compared to the cosmetic composition of Comparative Example, the multilayer lamellar nanoliposome particles containing ceramide are more stably dispersed, and the multilayer lamellar nanoliposome particles containing ceramide are It was found that the internal negative charge and the external positive charge form a strong static periodic interaction, for example, an ionic bond, forming a more stable liposome structure.

Experimental Example 4. Microscopic analysis of a cosmetic composition containing multi-layered lamellar nano-liposomes containing ceramide

In this experimental example, microscopic imaging was performed to evaluate the stability of the cosmetic composition including the multi-lamellar nanoliposome containing ceramide.

Specifically, microscopy was performed on the cosmetic compositions of Examples 1 to 2 and Comparative Examples 5 to 6 in Table 1 on the 30th day of storage using a microscope (Olimpus BX53M).

As a result, as shown in FIG. 5, the cosmetic compositions of Examples 1 and 2, compared to the cosmetic compositions of Comparative Examples 5 and 6, contain multi-layered lamellar nanoliposome particles containing smaller and more uniform ceramides. And, it was confirmed that the particles were very stably dispersed in the cosmetic composition.

Through this experimental example, in the case of the cosmetic composition of Example prepared above, together with ceramide, about 1 to 10% by weight of an amphoteric surfactant (specifically, lecithin); about 12 to 24% by weight of a nonionic surfactant (specifically, polyglycerin fatty acid ester and glyceryl citrate/lactate/linoleate/oleate); And an anionic surfactant (specifically, sodium lauroyl glutamate) about 0.1 to 5% by weight, polyglycerin fatty acid ester and glyceryl citrate / lactate / linoleate / oligo constituting the nonionic surfactant By including 8 in a weight ratio of about 4 to 11:9 to 11 or about 1:0.9 to 1.1, ceramide-containing multilayer lamellar nanoliposome particles having a uniform and very small size were stably formed, and the liposome particles were a cosmetic composition. It was confirmed that it was very stably dispersed in For this reason, it was found that the cosmetic composition of Example prepared above can exhibit excellent emulsification, solubilization, or dispersion stability while containing a high concentration of ceramide of about 20% by weight.

On the other hand, the amphoteric surfactant; nonionic surfactants; And a comparison in which the weight% of the anionic surfactant is out of the weight%, or the weight ratio of the polyglycerin fatty acid ester and glyceryl citrate / lactate / linoleate / oleate constituting the nonionic surfactant is out of the weight ratio In the case of the cosmetic composition of Example, it was confirmed that the particle size was not uniform and relatively large, and the dispersion of the particles was very unstable. Through this, it was found that the cosmetic composition of Comparative Example did not form ceramide-containing multilayer lamellar nanoliposomes or was formed very unstable. Could know.

Experimental Example 5. Analysis of skin permeability of a cosmetic composition containing multilayer lamellar nanoliposomes containing ceramide

In this experimental example, a Franz Cell Diffusion system was used to evaluate the skin permeability of the cosmetic composition including the multilayer lamellar nanoliposomes containing ceramide.

Specifically, after removing the subcutaneous fat and tissue except for the dermis from the skin extracted from the abdomen of mice killed by cervical dislocation (ICR outbred albino mice, 12 weeks old, 50g, female), it was cut to a size of about 1.5 cm horizontally and vertically. and used it. A Franz cell diffusion system was prepared by fixing the skin between a donor and a receptor phase with the stratum corneum facing upward. About 5 ml of the receptor phase was administered to the Franz cell diffusion system, and the temperature was maintained at about 37±0.5° C. using a constant temperature water bath, and stirred at about 150 rpm for about 2 hours. After about 0.2 ml of each sample was applied to the epidermal surface of the donor, about 0.3 ml of the recipient phase was collected each time at 0, 1, 2, 3, 4, and 5 hours. The collected receptor phase was subjected to vortex mixing for about 2 minutes and subjected to quantitative analysis after centrifugation.

As a result, as shown in FIG. 6, it was confirmed that the cosmetic compositions of Examples 1 and 2 exhibited superior skin permeability compared to the cosmetic compositions of Comparative Examples 5 and 6. In particular, it was confirmed that the cosmetic composition of Example 2 exhibited significantly superior skin permeability by about 4 times or more compared to the cosmetic composition of Comparative Examples 5 to 6.

Through this experimental example, in the case of the cosmetic composition of Example prepared above, together with ceramide, about 1 to 10% by weight of an amphoteric surfactant (specifically, lecithin); about 12 to 24% by weight of a nonionic surfactant (specifically, polyglycerin fatty acid ester and glyceryl citrate/lactate/linoleate/oleate); And an anionic surfactant (specifically, sodium lauroyl glutamate) about 0.1 to 5% by weight, polyglycerin fatty acid ester and glyceryl citrate / lactate / linoleate / oligo constituting the nonionic surfactant By including 8 in a weight ratio of about 4 to 11: 9 to 11 or about 1: 0.9 to 1.1, ceramide-containing multilayer lamellar nanoliposome particles having a uniform and very small size were stably formed. It was found that the skin permeability of the liposome particles significantly increased compared to the cosmetic composition.

The above description of the present invention is for illustrative purposes, and those skilled in the art can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting.

Claims (15)

A cosmetic composition comprising a ceramide, an amphoteric surfactant, a nonionic surfactant, and an anionic surfactant. The cosmetic composition according to claim 1, wherein the composition comprises liposome particles containing the ceramide, the amphoteric surfactant, the nonionic surfactant, and the anionic surfactant. The cosmetic composition according to claim 2, wherein the liposome particles have a multilayer lamellar membrane structure and a core for collecting the ceramide. The cosmetic composition according to claim 1, wherein the composition comprises 0.001% to 30% by weight of the ceramide. The cosmetic composition according to claim 1, wherein the composition comprises 1% to 10% by weight of the amphoteric surfactant. The cosmetic composition according to claim 1, wherein the composition comprises 12% to 24% by weight of the nonionic surfactant. The cosmetic composition according to claim 1, wherein the composition comprises 0.1% to 5% by weight of the anionic surfactant. The cosmetic composition according to claim 1, wherein the nonionic surfactant comprises polyglycerin fatty acid ester and glyceryl citrate/lactate/linoleate/oleate. The cosmetic composition according to claim 8, wherein the composition comprises the polyglycerin fatty acid ester and the glyceryl citrate/lactate/linoleate/oleate in a weight ratio of 4 to 11:9 to 11. The cosmetic composition according to claim 8, wherein the polyglycerin fatty acid ester includes polyglyceryl-10 stearate, polyglyceryl-10 laurate, or a combination thereof. The method according to claim 10, wherein the composition comprises the polyglyceryl-10 stearate, the polyglyceryl-10 laurate, and the glyceryl citrate/lactate/linoleate/oleate in a ratio of 1:1:1.5 to 5. That which is contained in a weight ratio, a cosmetic composition. The cosmetic composition according to claim 2, wherein the liposome particles have an average particle diameter of 50 nm to 500 nm. The cosmetic composition according to claim 1, wherein the composition has a zeta potential value of -60 mV to -30 mV. The cosmetic composition according to claim 1, wherein the composition exhibits a temperature stability improvement effect, an emulsion stability improvement effect, a solubilization stability improvement effect, a dispersion stability improvement effect, or a skin permeability improvement effect. A method for preparing a cosmetic composition comprising mixing purified water, ceramide, an amphoteric surfactant, a nonionic surfactant, and an anionic surfactant.