KR20230115701A - A new plant type rice having high calcium contents and cultivation method - Google Patents

Abstract

The present invention relates to a new rice variety high in calcium 'Miryang No. 359' and a method for cultivating the same.
'Miryang No. 359' according to the present invention is resistant to blast and striped leaf blight, and its calcium content is about 2.5 times higher than that of Nampyeong, so it can be usefully used as functional rice.

Description

A new plant type rice having high calcium contents and cultivation method}

The present invention relates to a new rice variety high in calcium 'Miryang No. 359' and a method for cultivating the same.

Rice is traditionally the staple food in Korea, but due to changes in consumption conditions, the demand for cooked rice has been continuously declining since the 1980s. Therefore, it is important to develop rice varieties for various uses in order to enhance the competitiveness of domestic rice and expand consumption.

Accordingly, studies are being actively conducted to add special ingredients to rice, which is a staple food in Asia, to make it a health food, or to obtain a therapeutic effect by eating rice. For example, the Rural Development Administration developed 'Miryang 263', a giant heukchal embryo rice with a high content of anthocyanin and rich in various functional substances such as GABA (γ-aminobutyric acid), which is 2.8 times larger than normal rice. There is (Korean Patent Registration No. 10-1201436).

Under this background, the present inventors have made diligent efforts to develop functional rice varieties, and as a result, 'Milyang No. 359', which was cultivated by crossing Junam, a mid-late maturing rice, with rice selected by treating Dharial with EMS (Ethyl-methane-sulfonate), was The present invention was completed by confirming that it was resistant to striped leaf blight and that the calcium content was about 2.5 times higher than that of Nampyeong, a late-season rice.

One object of the present invention is to provide a rice variety 'Miryang 359' of the Junam hybridization line having a high calcium content, deposited under accession number KACC 98126P.

Another object of the present invention is to provide a method for breeding rice varieties having a high calcium content.

Another object of the present invention is to provide a food composition containing the rice varieties or seeds thereof as an active ingredient.

A detailed description of this is as follows. Meanwhile, each description and embodiment disclosed in the present invention may also be applied to each other description and embodiment. That is, all combinations of the various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be seen that the scope of the present invention is limited by the specific descriptions described below.

In addition, those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Also, such equivalents are intended to be included in this invention.

One aspect of the present invention provides a Junam hybrid line 'Milyang 359' rice variety having a high calcium content, deposited under Accession No. KACC 98126P.

The rice variety according to the present invention is a variety selected by crossing an EMS (Ethyl-methane-sulfonate) treated Dharial mutant fixed line with a Junam variety, and the variety was named 'YR30098-4B-377' line. In December 2018, the line was judged to be suitable for the selection requirements for mid-late maturation varieties, and was selected as 'Miryang No. 359', an excellent line in the 2019 rice new regional adaptation test announcement. In addition, the YR30098-4B-377 strain was deposited with the National Academy of Agricultural Sciences of the Rural Development Administration and was given the deposit number KACC 98126P on October 12, 2021.

The 'Miryang No. 359' rice variety of the present invention may have a calcium content that is more than twice as high as that of the parental or mid-late variety, specifically more than three times or more than four times.

In one embodiment of the present invention, as a result of confirming the amylose and calcium (Ca) contents of 'Milyang 359' and Nampyeong, a mid-late maturing rice, 'Miryang 359' has an amylose content of 6.0%, which is glutinous rice, and the calcium content is higher than that of Nampyeong. about 2.5 times higher (Table 3).

In one embodiment of the present invention, as a result of confirming the brown rice color of 'Miryang 359' and Nampyeong, a medium-late rice variety, 'Miryang 359' was brown in color (FIG. 2).

In another embodiment of the present invention, after cultivating 'Milyang 359' in Miryang, Jeonju, Jeonbuk, Jeonnam, Chungbuk, and Chungnam, respectively, the calcium content was checked. Although there is a difference, nevertheless, the calcium content was 2 to 4 times higher than that of general rice such as Nampyeong and Hwaseong (Fig. 1).

The 'Miryang No. 359' rice variety of the present invention may have one or more of the following characteristics:

(a) Departure period: August 20 ± 10 days

(b) Soy sauce (cm): 67±10

(c) Head length (cm): 20±10

(d) sorghum (pieces): 15±5

(e) Number of grains per number: 92±20

(f) Weight of a thousand grains (g): 15.9±5.0

(g) Brown rice yield (kg/10a): 450±100.

The 'Miryang No. 359' rice variety of the present invention may be resistant to blast disease.

The 'Miryang No. 359' rice variety of the present invention may be resistant to striped leaf blight.

In one embodiment of the present invention, 'Milyang No. 359' was resistant to blast and striped leaf blight and showed susceptibility to white leaf blight (Table 2).

The 'Miryang No. 359' rice variety of the present invention may be glutinous rice.

In one embodiment of the present invention, 'Milyang No. 359' was confirmed to be glutinous with an amylose content of 6.0% (Table 3).

Another aspect of the present invention provides a method for breeding rice varieties having a high calcium content.

The method is (a) immersing Dharial rice seeds in a dilute solution of EMS (Ethyl-methane-sulfonate), germinating and harvesting M1 seeds from cultivated plants (M0) based on the group breeding method to advance to the M2 generation and again Establishing a fixed mutant line by advancing to the M5 generation based on the phylogenetic breeding method; and (b) crossing the established mutant line with the Junam cultivar.

Terms used herein are as described above.

In the step (a), Dharial rice seeds are immersed in a dilute solution of EMS (Ethyl-methane-sulfonate), and the M1 seeds harvested from the plant (M0) grown by germination are advanced to the M2 generation based on the collective breeding method, and again It may be to establish a fixed mutant strain by advancing up to the M5 generation based on the phylogenetic breeding method.

The treatment concentration of EMS (Ethyl-methane-sulfonate) in step (a) is not particularly limited thereto, but as an example, it may be about 10 to 200 mM, and as another example, it may be about 50 to 180 mM, and another As an example, it may be about 150 mM.

The immersion temperature in step (a) is not particularly limited thereto, but may be, for example, about 15 to 30 °C, and as another example, 25 °C.

The immersion time of the step (a) is not particularly limited thereto, but as an example, it may be about 1 to 24 hours, as another example, it may be about 6 to 18 hours, and as another example, about 12 hours It can be.

In the present invention, the term “about” may be preceded by a specific numerical value. As used in this application, the term "about" includes not only the exact number that follows the term, but also a range that is or is close to that number. It can be determined whether the number is close to or nearly the specific number mentioned, given the context in which it is presented. As an example, the term “about” can refer to a range of -10% to +10% of a numerical value. As another example, the term "about" can refer to a range of -5% to +5% of a given numerical value. However, it is not limited thereto.

Step (b) may be to cross the established mutant line with the Joonam variety.

In the present invention, steps (a) and (b) may be carried out with appropriate changes according to changes in the characteristics of the mutant strain.

Another aspect of the present invention provides a food composition comprising the rice variety of the present invention, its seeds or extract thereof as an active ingredient.

Terms used herein are as described above.

Specifically, the rice variety is 'Milyang No. 359', as described above.

The food composition of the present invention includes all types of rice processed foods, functional foods, nutritional supplements, health functional foods, and food additives. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

For example, processed rice foods include rice cakes and noodles (tteok, rice cake soup, rice cakes with rice cakes, noodles, ramen, noodles, fresh noodles, traditional rice cakes), rice crackers (biscuits, biscuits, snacks, Korean snacks, fried rice, and fried rice), and rice flour. (raw flour, alpha flour, wet flour), alcoholic beverages (tak-yak wine, soju, beer, rice wine), seasoning foods (taffy, fermented soy sauce, vinegar) and other processed rice products (porridge, Sikhye, snack additives, skewers, seafood, Rice beverage, rice bread, processed rice) can be prepared by processing the rice varieties or seeds thereof provided in the present invention.

In the present invention, "functional food" is the same term as food for special health use (FoSHU), medicine processed to efficiently display bioregulatory functions in addition to nutritional supply, Means food with high medical effect. Here, "function (sex)" means to obtain useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions.

As an essential ingredient that can be included in the food composition of the present invention, there is no particular limitation on other ingredients other than the 'Milyang No. 359' rice variety, its seeds or its extract, and, like conventional foods, various herbal extracts, food supplement additives or Natural carbohydrates and the like may be contained as additional components. In addition, the food auxiliary additives include food auxiliary additives common in the art, such as flavoring agents, flavoring agents, coloring agents, fillers, stabilizers, and the like.

The food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and ingredients commonly added in the art during the preparation. In addition, the formulation of the food may also be prepared without limitation as long as the formulation is recognized as a food. The food composition of the present invention can be prepared in various forms of dosage form, and unlike general medicines, it can have the advantage of being free from side effects that can occur when taking medicines for a long time or having excellent portability using food as a raw material.

The content of the plant, its seed or its extract, which may be included in the food composition of the present invention, may be, for example, 0.01 to 95% by weight of the finally prepared food, but is not limited thereto.

'Milyang No. 359', a new variety of rice high in calcium according to the present invention, is resistant to blast and striped leaf blight, and has a calcium content about 2.5 times higher than that of Nampyeong, so it can be usefully used as functional rice.

1 is a diagram showing the calcium content of 'Miryang No. 359' by cultivation area.
2 is a photograph of seeds of Jeongjo (A: Nampyeong, B: Miryang No. 359) and brown rice (C: Nampyeong, D: Miryang No. 359).
3 is a picture of the mature stage of 'Miryang 359'.

Hereinafter, the present invention will be described in more detail through Examples and Experimental Examples. However, these Examples and Experimental Examples are intended to illustrate the present invention, and the scope of the present invention is not limited to these Examples and Experimental Examples.

Example 1: Selection of new rice varieties

Dharial rice seeds were treated with EMS (Ethyl-methane-sulfonate) as a mutagen (soaked in 150 mM EMS at 25 °C for 12 hours) to secure mutant progeny lines. Two seeds were harvested from each generation of plants from M1 to M2 for generational progress, and stationary lines were cultivated while advancing generations based on the systematic breeding method from M3 to M5 generations. In the M5 generation, it was determined that the individuals in each lineage were genetically fixed by showing a uniform phenotype, and after confirming the lineage, the EMSD-2B-20-1-1 line was selected.

Next, the F1 seed obtained by crossing the selected EMSD-2B-20-1-1 line with Junam, a mid-late rice variety, was published on the packaging and named YR30098. After that, the YR30098-4B-377 line with excellent plant shape and high calcium content was selected by developing it as a line after the 4th generation through a group breeding method.

Example 2: Characterization of new rice varieties

2-1: Agronomic characteristics

The YR30098-4B-377 line selected in Example 1 was published on the test field of the National Institute of Food Science and Southern Crops (Miryang) under normal season fertilization conditions, and observed major agronomic characteristics such as harvest period, soy sauce, water length, number of grains per grain, and weight of thousand grains. did

The agronomic characteristics of the YR30098-4B-377 line are shown in Table 1 below.

A photograph of the maturity of YR30098-4B-377 ('Miryang No. 359') is shown in Figure 3.

family name water dispenser
(Mon. Sun) soy sauce
(cm) stripe
(cm) sugarcane
(dog) Number of grains per unit
(dog) full-maturity rate
(%) Cheonlip Middle School
(g) Brown rice quantity
(kg/10a) Miryang 359 8.20 67 20 15 92 - 15.9 450

2-2: disease resistance

As a result of confirming the disease resistance of the YR30098-4B-377 line to blast, white leaf blight and striped leaf blight, as shown in Table 2 below, the YR30098-4B-377 line is resistant to blast and striped leaf blight and shows susceptibility to white leaf blight. was

family name blast
(0-9)
(Miryang, Sangju) Hin leaf blight
(K ₁ -K _3a ) stripe
leaf blight Miryang 359 1, 7 7, 7, 7, 7 river Nam Pyeong 4, 5 7, 7, 7, 7 river

2-3: Min quality characteristics

As a result of confirming the amylose and calcium (Ca) contents of the YR30098-4B-377 line and Nampyeong, a mid-late rice variety, as shown in Table 3 below, YR30098-4B-377 has an amylose content of 6.0%, and the calcium content is higher than that of Nampyeong. about 2.5 times higher.

Pictures of seeds of rice (A: Nampyeong, B: YR30098-4B-377 ('Miryang No. 359')) and brown rice (C: Nampyeong, D: Miryang No. 359) containing YR30098-4B-377 are shown in FIG. did

family name amylose content
(%) Ca content
(mg/g) note Miryang 359 6.0 0.32 brown Nam Pyeong 19.1 0.13 -

Example 3: Calcium content analysis by cultivation area of new rice varieties

After cultivating the YR30098-4B-377 line selected in Example 1 in Miryang, Jeonju, Jeonbuk, Jeonnam, Chungbuk and Chungnam, respectively, the calcium content was confirmed.

As a result, as shown in Figure 1, the YR30098-4B-377 line has a slight difference in calcium content by cultivation area, but nevertheless, the calcium content is 2 to 4 times higher than that of general rice such as Nampyeong and Hwaseong.

The YR30098-4B-377 line was judged to be suitable for the selection requirements for mid-late maturation varieties in December 2018, and was selected as ‘Miryang No. 359’, an excellent line in the announcement of the new regional adaptation test for rice in 2019.

In addition, the YR30098-4B-377 strain was deposited with the National Academy of Agricultural Sciences of the Rural Development Administration and was given the deposit number KACC 98126P on October 12, 2021.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing its technical spirit or essential features. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Rural Development Administration National Institute of Agricultural Science KACC98126P 20211012

Claims (7)

Rice cultivar 'Milyang 359' of the Junam hybridization line with high calcium content deposited under accession number KACC 98126.
The rice variety according to claim 1, wherein the rice variety has a calcium content more than twice as high as that of the parental or mid-late variety.
The rice variety according to claim 1, wherein the rice variety has the following characteristics:
(a) Departure period: August 20 ± 10 days
(b) Soy sauce (cm): 67±10
(c) Head length (cm): 20±10
(d) sorghum (pieces): 15±5
(e) Number of grains per number: 92±20
(f) Weight of a thousand grains (g): 15.9±5.0
(g) Brown rice yield (kg/10a): 450±100.
The rice variety according to claim 1, wherein the rice variety is resistant to blast.
The rice variety according to claim 1, wherein the rice variety has resistance to striped leaf blight.
(a) Dharial rice seeds are immersed in a dilute solution of EMS (Ethyl-methane-sulfonate), and M1 seeds harvested from plants grown by germination are advanced to the M5 generation based on the systematic breeding method to establish a fixed mutant strain step; and
(b) crossing the established mutant line with the Junam variety; a method for breeding rice varieties with high calcium content, including.
Claims 1 to 5 of any one of rice varieties or a food composition containing its seeds as an active ingredient.