KR20230108402A - Method for preparing liquefied composition with nipa fruticans wurmb - Google Patents

Method for preparing liquefied composition with nipa fruticans wurmb Download PDF

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KR20230108402A
KR20230108402A KR1020220003754A KR20220003754A KR20230108402A KR 20230108402 A KR20230108402 A KR 20230108402A KR 1020220003754 A KR1020220003754 A KR 1020220003754A KR 20220003754 A KR20220003754 A KR 20220003754A KR 20230108402 A KR20230108402 A KR 20230108402A
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flower
buds
composition
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stalks
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장훈
문민지
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/42Preservation of non-alcoholic beverages
    • A23L2/44Preservation of non-alcoholic beverages by adding preservatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof
    • A23L27/11Natural spices, flavouring agents or condiments; Extracts thereof obtained by solvent extraction
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3472Compounds of undetermined constitution obtained from animals or plants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/10Preserving against microbes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health
    • A23V2200/302Foods, ingredients or supplements having a functional effect on health having a modulating effect on age
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health
    • A23V2200/324Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health
    • A23V2200/334Foods, ingredients or supplements having a functional effect on health treating the effects of consuming alcohol, narcotics or other addictive behavior, e.g. treating hangover or reducing blood alcohol levels

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

본 발명은 니파야자 꽃대 및 꽃봉오리의 알코올추출물을 이용한 식품첨가물용 조성물의 제조방법에 관한 것으로, 더욱 상세하게는 니파야자 꽃대와 꽃봉오리를 세척하는 단계와, 상기 세척된 꽃대와 꽃봉오리를 끓는 물에 10~40분간 데치는 단계와, 상기 데친 꽃대와 꽃봉오리를 건조하는 단계와, 상기 건조된 건조물과 말라바시금치를 혼합하는 단계와, 상기 혼합된 혼합물을 로스팅하는 단계와, 상기 로스팅된 혼합물을 분쇄하는 단계와, 상기 분쇄된 혼합물에 알코올 또는 알코올 수용액을 가하고 20~40℃에서 1~5시간 추출한 후, 여과 및 건조하는 단계를 포함하는 것을 특징으로 한다. 본 발명에 의하면, 각종 영양소가 풍부하고, 항산화, 항염 활성 및 해독작용이 우수한 식품첨가물용 조성물을 제조함으로써, 다양한 식품에 상기 조성물을 첨가할 수 있게 되어 소비자의 건강 유지를 도우며, 식품의 풍미를 개선할 수 있는 기능성 식품을 제공할 수 있다는 장점이 있다. 또한, 식품의 기능성 및 영양학적 가치를 우수하게 하는 것은 물론, 감칠맛을 높여주고, 보존성을 좋게 하여 별도의 화학 첨가제 사용을 방지할 수 있는 효과가 있다. The present invention relates to a method for preparing a composition for food additives using an alcohol extract of nipa palm flower stalks and flower buds, and more particularly, to a method comprising the steps of washing the flower stalks and flower buds of nipa palm, and boiling the washed flower stalks and flower buds. Blanching in water for 10 to 40 minutes, drying the blanched peduncles and flower buds, mixing the dried dried material with Malabar spinach, roasting the mixed mixture, and roasting the roasted mixture It is characterized in that it comprises the step of pulverizing, adding alcohol or an aqueous alcohol solution to the pulverized mixture, extracting at 20 to 40 ° C. for 1 to 5 hours, and then filtering and drying. According to the present invention, by preparing a composition for food additives rich in various nutrients and excellent in antioxidant, anti-inflammatory activity and detoxification, it is possible to add the composition to various foods, thereby helping consumers maintain health and enhancing the flavor of food. It has the advantage of being able to provide functional foods that can be improved. In addition, there is an effect of preventing the use of separate chemical additives by improving the functionality and nutritional value of food, as well as enhancing umami and improving preservation.

Description

니파야자 꽃대 및 꽃봉오리의 알코올추출물을 이용한 식품첨가물용 조성물의 제조방법{METHOD FOR PREPARING LIQUEFIED COMPOSITION WITH NIPA FRUTICANS WURMB}Method for preparing a composition for food additives using alcohol extracts of flower stalks and flower buds of nipa palm {METHOD FOR PREPARING LIQUEFIED COMPOSITION WITH NIPA FRUTICANS WURMB}

본 발명은 니파야자 꽃대 및 꽃봉오리의 알코올추출물을 이용한 식품첨가물용 조성물의 제조방법에 관한 것으로, 더욱 상세하게는 니파야자 꽃대 및 꽃봉오리를 활용하여 항산화 활성, 항염증 활성 및 해독작용이 우수하면서도, 식품에 첨가시 그 풍미를 개선하고, 보존성을 개선할 수 있는 식품첨가물용 조성물의 제조방법에 관한 것이다. The present invention relates to a method for preparing a composition for food additives using an alcohol extract of nipa palm flower stems and flower buds, and more particularly, to a method for preparing a composition for food additives using the flower stems and flower buds of nipa palm, which has excellent antioxidant activity, anti-inflammatory activity and detoxification activity, , It relates to a method for producing a composition for food additives that can improve the flavor and improve preservation when added to food.

최근 소비자들은 의약품 형태의 기능성만을 추구하기보다 맛과 기능성을 동시에 갖는 의약건강식품(neutraceuticals)을 더욱 많이 요구하고 있으므로, 식미를 개선하고 소비자가 쉽게 접근할 수 있는 제품 개발이 필요하다.Recently, consumers are demanding more neutraceuticals that have both taste and functionality rather than only pursuing functionality in the form of medicines, so it is necessary to develop products that improve taste and are easily accessible to consumers.

이러한 제품으로는 식품첨가물이 가장 일반적인바, 대한민국 공개특허 제10-2011-0026570호에서는 홍삼, 고본, 겨우살이, 감초 및 프로폴리스의 추출물을 포함하는 식품첨가물이 제안되었다. 또한, 대한민국 등록특허 제10-2204948호에서는 다시마를 이용한 식품첨가물의 제조방법이 제안되었다.Food additives are the most common of these products, and Korean Patent Publication No. 10-2011-0026570 proposes food additives containing extracts of red ginseng, gobon, mistletoe, licorice and propolis. In addition, Korean Patent Registration No. 10-2204948 proposed a method for producing food additives using kelp.

이러한 선행특허들에서는 항산화활성이 우수하고, 영양성분이 풍부하여 소비자의 건강유지를 도모하였다. These prior patents have excellent antioxidant activity and are rich in nutrients, promoting the maintenance of health of consumers.

또한, 대한민국 등록특허 제10-1710194호에서는 니파야자의 꽃대를 이용한 음료첨가물용 조성물을, 대한민국 등록특허 제10-2158291호에서는 니파야자의 꽃봉오리를 이용한 음료첨가물용 조성물을 개시하여 그 기능성을 개선하였다.In addition, Korean Patent Registration No. 10-1710194 discloses a composition for beverage additives using flower stalks of the nipa palm, and Korean Patent No. 10-2158291 discloses a composition for beverage additives using flower buds of the nipa palm, improving their functionality. did

그러나 상기 선행특허들의 어디에서도 니파야자 꽃대, 꽃봉오리 및 말라바시금치를 이용하여 식품첨가물을 제조하는 방법을 찾아볼 수 없다.However, in none of the prior patents, a method for preparing a food additive using nipa palm flower stalks, flower buds, and Malabar spinach cannot be found.

KRKR 10-2011-0026570 10-2011-0026570 AA KRKR 10-2204948 10-2204948 B1B1 KRKR 10-1710194 10-1710194 B1B1 KRKR 10-2158291 10-2158291 B1B1

따라서, 본 발명의 목적은 니파야자 꽃대, 꽃봉오리 및 말라바시금치를 이용하여 항산화, 항염 활성 및 해독작용이 우수한 식품첨가물용 조성물을 제조함으로써, 각종 식품, 특히 음료의 기능성 첨가물로 활용할 수 있도록 하는 식품첨가물용 조성물을 제공하는 데 있다. Accordingly, an object of the present invention is to prepare a composition for food additives having excellent antioxidant, anti-inflammatory activity and detoxification activities using nipa palm flower stalks, flower buds and Malabar spinach, so that it can be used as a functional additive for various foods, especially beverages. It is to provide a composition for food additives.

또한, 식품의 제조시 풍미의 개선 및 보존성 향상을 위한 천연 첨가물로의 활용이 가능하여 몸에 좋지 않은 화학제의 첨가를 지양할 수 있도록 하는 식품첨가물용 조성물을 제공하는 데 있다. In addition, to provide a composition for food additives that can be used as a natural additive to improve flavor and improve preservation during food production, thereby avoiding the addition of unhealthy chemicals.

상기한 목적을 달성하기 위한 본 발명의 니파야자 꽃대 및 꽃봉오리의 알코올추출물을 이용한 식품첨가물용 조성물의 제조방법은, 니파야자 꽃대와 꽃봉오리를 세척하는 단계와, 상기 세척된 꽃대와 꽃봉오리를 끓는 물에 10~40분간 데치는 단계와, 상기 데친 꽃대와 꽃봉오리를 건조하는 단계와, 상기 건조된 건조물과 말라바시금치를 혼합하는 단계와, 상기 혼합된 혼합물을 로스팅하는 단계와, 상기 로스팅된 혼합물을 분쇄하는 단계와, 상기 분쇄된 혼합물에 알코올 또는 알코올 수용액을 가하고 20~40℃에서 1~5시간 추출한 후, 여과 및 건조하는 단계를 포함하는 것을 특징으로 한다.In order to achieve the above object, the method for preparing a composition for food additives using an alcohol extract of nipa palm flower stalks and flower buds of the present invention includes the steps of washing the flower stalks and flower buds of nipa palm, and the washed flower stalks and flower buds. Blanching in boiling water for 10 to 40 minutes, drying the blanched peduncles and flower buds, mixing the dried dried material with Malabar spinach, roasting the mixed mixture, It is characterized in that it comprises the step of pulverizing the mixture, adding alcohol or an aqueous alcohol solution to the pulverized mixture, extracting at 20 to 40 ° C. for 1 to 5 hours, and then filtering and drying.

상기 건조된 건조물과 말라바시금치를 혼합하는 단계에서, 원추리의 꽃봉오리를 추가로 혼합하는 것을 특징으로 한다.In the step of mixing the dried dried product and Malabar spinach, it is characterized in that the flower buds of daylily are additionally mixed.

상기 건조된 건조물과 말라바시금치를 혼합하는 단계에서, 곤달비 잎을 추가로 혼합하는 것을 특징으로 한다.In the step of mixing the dried dried material and Malabar spinach, it is characterized in that the gondalbi leaves are additionally mixed.

상기 세척된 꽃대와 꽃봉오리를 끓는 물에 10~40분간 데치는 단계 후, 상기 데친 꽃대와 꽃봉오리에 탱자 효소를 첨가하고 1~5일간 1~5℃에서 숙성하는 단계를 더 포함하는 것을 특징으로 한다.After the step of blanching the washed peduncles and buds in boiling water for 10 to 40 minutes, adding Tangja enzyme to the blanched peduncles and buds and aging at 1 to 5 ° C for 1 to 5 days. do.

상기 탱자 효소는 탱자의 열매에 설탕을 1:2~10 중량비로 첨가하여 10~25℃에서 10~100일 동안 숙성하고 여과하여 제조된 것임을 특징으로 한다.The Tangja enzyme is characterized in that it is prepared by adding sugar to the fruit of Tangja at a weight ratio of 1:2 to 10, aging at 10 to 25 ° C. for 10 to 100 days, and filtering.

상기 건조된 건조물과 말라바시금치를 혼합하는 단계에서, 녹차 잎을 추가로 혼합하는 것을 특징으로 한다. In the step of mixing the dried dried product and Malabar spinach, green tea leaves are further mixed.

본 발명에 의하면, 각종 영양소가 풍부하고, 항산화, 항염 활성 및 해독작용이 우수한 식품첨가물용 조성물을 제조함으로써, 다양한 식품에 상기 조성물을 첨가할 수 있게 되어 소비자의 건강 유지를 도우며, 식품의 풍미를 개선할 수 있는 기능성 식품을 제공할 수 있다는 장점이 있다.According to the present invention, by preparing a composition for food additives rich in various nutrients and excellent in antioxidant, anti-inflammatory activity and detoxification, it is possible to add the composition to various foods, thereby helping consumers maintain health and enhancing the flavor of food. It has the advantage of being able to provide functional foods that can be improved.

또한, 식품의 기능성 및 영양학적 가치를 우수하게 하는 것은 물론, 감칠맛을 높여주고, 보존성을 좋게 하여 별도의 화학 첨가제 사용을 방지할 수 있는 효과가 있다. In addition, there is an effect of preventing the use of separate chemical additives by improving the functionality and nutritional value of food, as well as enhancing umami and improving preservation.

도 1은 본 발명에 의한 식품첨가물용 조성물의 제조공정을 나타낸 도면.
도 2 및 도 3은 본 발명의 시험예 2에 따른 결과를 나타낸 그래프.
1 is a view showing a manufacturing process of a composition for food additives according to the present invention.
2 and 3 are graphs showing the results according to Test Example 2 of the present invention.

이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.

본 발명의 식품첨가물용 조성물은, 각종 식품에 항산화 및 항염활성을 부여하는 것은 물론, 해독작용을 부여하고, 감칠맛을 높여주고, 그 풍미를 개선하며, 보존성을 좋게 하는 것으로, 즉석에서 제조되어 제공되는 식품의 첨가물로 활용될 수도 있으며, 가공식품의 첨가물로서도 활용될 수 있는 것으로, 그 활용을 제한하지 않는다. 즉, 가장 간편하게는 시판되는 음료, 유제품 등, 또는 물에 본 발명의 조성물을 즉석에서 혼합하여 섭취하는 것이고, 이러한 형태가 아닌 가공음료 또는 각종 가공식품의 제조공정 중 본 발명의 조성물을 혼합하여 제조할 수도 있는 것이다. 아울러, 시판 화학조미료를 대체하여 사용함으로써, 식품의 감칠맛을 높일 수도 있는 것으로, 그 사용을 제한하지 않는다.The composition for food additives of the present invention not only imparts antioxidant and anti-inflammatory activity to various foods, but also imparts detoxification, enhances umami, improves flavor, and improves preservation, and is prepared and provided on the spot. It can also be used as an additive for processed foods, and it can also be used as an additive for processed foods, and its use is not limited. That is, the most convenient way is to immediately mix the composition of the present invention with commercially available beverages, dairy products, or water, and mix the composition of the present invention during the manufacturing process of non-processed beverages or various processed foods. It can be done. In addition, by replacing and using commercially available chemical seasonings, the umami of food can be enhanced, and its use is not limited.

또한, 본 발명에 의해 제조된 조성물의 사용량은 제한하지 않는바, 예시적으로 식품 100중량부에 대해 0.01~10중량부만큼 사용 가능함을 밝혀둔다.In addition, the amount of the composition prepared by the present invention is not limited, and it is exemplified that 0.01 to 10 parts by weight can be used for 100 parts by weight of food.

이러한 본 발명의 식품첨가물용 조성물은 니파야자를 이용하여 제조하는 것이다.The composition for food additives of the present invention is prepared using nipa palm.

여기서, 상기 니파야자(학명 : NIPA FRUTICANS WURMB)는 인도, 말레이시아를 비롯한 동남아시아 및 오스트레일리아가 원산지이며, 맹그로브 지대 등의 습지에서 자란다. 뿌리는 땅속에서 여러 개로 갈라지며, 잎은 지면에서 뭉쳐나고 광택이 있는 녹색을 띤다. 그리고 꽃은 암수한그루이며, 지면의 잎겨드랑이에서 나오는데, 보통 10월에서 이듬해 6월 사이에 꽃대가 올라오고, 이에 꽃봉오리가 맺힌다.Here, the nipa palm (scientific name: NIPA FRUTICANS WURMB) is native to Southeast Asia and Australia, including India and Malaysia, and grows in wetlands such as mangroves. The roots are divided into several branches in the ground, and the leaves are gathered together on the ground and have a glossy green color. And the flowers are hermaphrodite, coming out from the leaf axils of the ground, and the peduncle rises between October and June of the following year, and buds form on it.

본 발명은 이러한 니파야자의 꽃대와 꽃봉오리를 채취하고, 이를 가공하여 식품첨가물용 조성물로 제조하는 것인데, 상기 꽃대와 꽃봉오리에는 폴리페놀 등의 항산화 성분이 풍부하고, 셀레늄 등의 각종 영양성분이 풍부할 뿐 아니라, 우수한 항염증 효능을 보이기 때문이다.The present invention is to collect the peduncle and bud of Nipa palm, and process them to prepare a composition for food additives. The peduncle and bud are rich in antioxidants such as polyphenols and contain various nutrients such as selenium. It is not only abundant, but also because it shows excellent anti-inflammatory effects.

다만, 이러한 니파야자 꽃대와 꽃봉오리만을 단독으로 사용하는 것보다 상기 꽃대 및 꽃봉오리와 함께 말라바시금치를 이용하는 것이 바람직한데, 이는 식품의 관능적 특성의 개선, 감칠맛 향상은 물론, 기능성을 고려할 때 현저히 상승된 작용효과를 나타내기 때문이다. 즉, 상기 니파야자의 꽃대와 꽃봉오리만을 사용할 경우 특유의 이취로 인하여 관능성이 좋지 못하나, 상기 말라바시금치는 이러한 니파야자 꽃대 및 꽃봉오리의 이취를 제거해주며, 감칠맛을 부여하고, 항산화력 역시 현저히 상승시켜준다. 특히, 말라바시금치는 니파야자와의 상호작용을 통해 글루타티온-S-트랜스퍼레이스와 퀴논 리덕타아제의 활성을 증가시켜 신체의 해독작용을 돕는다.However, it is preferable to use Malabar spinach together with the flower stem and flower bud rather than using only the flower stem and bud of nipa palm, which significantly improves the sensory characteristics of food, improves umami, as well as functionality. This is because it shows an increased action effect. That is, when only the flower stalk and bud of the nipa palm are used, the sensory properties are not good due to the peculiar odor, but the Malabar spinach removes the odor of the flower stalk and bud of the nipa palm, imparts a umami taste, and has antioxidant power. raises it significantly. In particular, Malabar spinach helps detoxify the body by increasing the activity of glutathione-S-transferase and quinone reductase through interaction with Nipa palm.

이러한 본 발명의 식품첨가물용 조성물의 제조방법은, 니파야자 꽃대와 꽃봉오리를 세척하는 단계와, 상기 세척된 꽃대와 꽃봉오리를 끓는 물에 10~40분간 데치는 단계와, 상기 데친 꽃대와 꽃봉오리를 건조하는 단계와, 상기 건조된 건조물과 말라바시금치를 혼합하는 단계와, 상기 혼합된 혼합물을 로스팅하는 단계와, 상기 로스팅된 혼합물을 분쇄하는 단계와, 상기 분쇄된 혼합물에 알코올 또는 알코올 수용액을 가하고 20~40℃에서 1~5시간 추출한 후, 여과 및 건조하는 단계를 포함하는 것을 특징으로 한다.The method for preparing the composition for food additives of the present invention comprises the steps of washing nipa palm flower stalks and flower buds, blanching the washed flower stalks and flower buds in boiling water for 10 to 40 minutes, and the blanched flower stalks and flower buds. drying, mixing the dried dried material with Malabar spinach, roasting the mixed mixture, grinding the roasted mixture, and adding alcohol or an alcohol aqueous solution to the ground mixture. It is characterized in that it comprises the steps of adding and extracting at 20 to 40 ° C. for 1 to 5 hours, followed by filtering and drying.

이하, 도 1을 참조하여 본 발명을 단계별로 상세히 설명한다.Hereinafter, the present invention will be described in detail step by step with reference to FIG.

니파야자 꽃대와 꽃봉오리를 세척하는 단계Steps to wash nipa palm flower stalks and flower buds

먼저, 니파야자의 꽃대와 꽃봉오리를 깨끗이 세척한다. 이때, 그 세척방법은 제한하지 않는바, 물, 알코올, 산 세척 등, 종래 게시된 방법을 이용하여 세척하는 정도면 족하다. First, wash the peduncle and flower buds of the nipa palm. At this time, the washing method is not limited, and washing using a conventionally posted method such as water, alcohol, acid washing, etc. is sufficient.

이때, 상기 꽃대는 3~10cm로 절단하여 세척한다. At this time, the peduncle is cut into 3 to 10 cm and washed.

상기 세척된 꽃대와 꽃봉오리를 끓는 물에 10~40분간 데치는 단계Blanching the washed flower stems and flower buds in boiling water for 10 to 40 minutes

다음으로, 상기 세척된 꽃대와 꽃봉오리를 끓는 물, 즉 100℃의 물에 10~40분간 데쳐내고, 상기 데친 꽃대와 꽃봉오리를 찬물에 담가 식힌다.Next, the washed peduncles and flower buds are blanched in boiling water, that is, 100 ° C. water for 10 to 40 minutes, and the blanched peduncles and flower buds are immersed in cold water to cool.

상기 데친 꽃대와 꽃봉오리를 건조하는 단계Drying the blanched peduncles and buds

그리고 상기 데친 꽃대와 꽃봉오리를 건져내고, 열풍건조, 냉풍건조, 일광에 의한 자연건조법 등을 통해 함수율이 5~10%가 되도록 건조한다. 이때, 상기 꽃대는 건조의 용이성을 위하여 길이방향, 즉 결대로 길게 찢어 건조할 수 있음은 당연하다. Then, the blanched peduncles and flower buds are taken out and dried so that the moisture content is 5 to 10% through hot air drying, cold air drying, and natural drying by sunlight. At this time, it is natural that the peduncle can be torn and dried in the longitudinal direction, that is, along the grain, for ease of drying.

상기 건조된 건조물과 말라바시금치를 혼합하는 단계Mixing the dried product with Malabar spinach

상기 건조된 건조물과 말라바시금치를 혼합한다. Mix the dried dried product and Malabar spinach.

이때, 상기 말라바시금치 역시 건조된 상태임이 바람직하나, 이를 제한하는 것은 아니다.At this time, it is preferable that the Malabar spinach is also in a dried state, but is not limited thereto.

상기 말라바시금치(Malaba Spinach)는 카로틴과 비타민C, 칼슘이 풍부하게 함유되어 있어, 식품 조성물의 영양성을 개선해줌은 물론, 관능적 특성 역시 높여주고, 항산화 활성을 더욱 높여준다. 아울러, 니파야자 꽃대, 꽃봉오리와의 상호작용으로 글루타티온-S-트랜스퍼레이스와 퀴논 리덕타아제의 활성을 증가시켜 신체의 해독작용을 돕는다.The Malaba spinach is rich in carotene, vitamin C and calcium, which improves the nutritional properties of food compositions, as well as enhances sensory properties and further enhances antioxidant activity. In addition, interaction with nipa palm flower stalks and flower buds increases the activity of glutathione-S-transferase and quinone reductase to help detoxify the body.

본 발명에서는 상기 말라바시금치의 잎을 이용한다.In the present invention, the leaves of the Malabar spinach are used.

상기 니파야자 꽃대 및 꽃봉오리의 건조물과 말라바시금치의 혼합비는 특별히 제한하지 않으나, 그 기능성 및 관능성을 고려할 때 상기 건조물 100중량부에 대하여 상기 말라바시금치 10~50중량부임이 바람직하다. The mixing ratio of the dried product of the nipa palm flower stalk and flower bud and the Malabar spinach is not particularly limited, but considering its functionality and functionality, it is preferable that the dried product is 10 to 50 parts by weight of the Malabar spinach based on 100 parts by weight of the dry product.

아울러, 상기 건조물 중 니파야자 꽃대와 꽃봉오리 간의 혼합비 역시 제한하지 않는데, 예시적으로 1:0.1~1 중량비 일 수 있다. In addition, the mixing ratio between the peduncle and bud of nipa palm among the dry matter is also not limited, and may be exemplarily 1:0.1 to 1 by weight.

상기 혼합된 혼합물을 로스팅하는 단계Roasting the mixed mixture

다음으로, 상기 혼합된 혼합물에 열을 가해 볶아준다. 상기 열을 가해 볶는 로스팅(roasting)은 재료 내부 조직에 물리적, 화학적 변화를 일으킴으로써 식품의 맛과 향을 좋게 하는 가공공정으로, 120~140℃에서 4~10분간 로스팅함으로써, 건조된 꽃대, 꽃봉오리, 말라바시금치의 향을 좋게 하여 식품의 풍미를 향상시키는 것이다.Next, heat is applied to the mixed mixture to stir-fry. Roasting by applying the heat is a processing process that improves the taste and aroma of food by causing physical and chemical changes in the internal structure of the material. It is to improve the flavor of food by improving the aroma of buds and Malabar spinach.

여기서, 상기 혼합된 혼합물은 밀폐된 용기에 넣고 열을 가해 볶아주는 것이 바람직한데, 이는 건조된 혼합물로부터 발생된 휘발성 성분들이 외부로 배출되는 것을 방지하기 위함이다. Here, it is preferable to put the mixed mixture in an airtight container and heat it to roast it, which is to prevent volatile components generated from the dried mixture from being discharged to the outside.

상기 로스팅된 혼합물을 분쇄하는 단계Grinding the roasted mixture

다음으로, 상기 로스팅된 혼합물을 10~300mesh 정도로 분쇄한다. 이는 분쇄를 통해 유용성분 용출을 용이하게 하기 위한 것이다. Next, the roasted mixture is pulverized to about 10 to 300 mesh. This is to facilitate the elution of useful components through grinding.

상기 분쇄된 혼합물에 알코올 또는 알코올 수용액을 가하고 20~40℃에서 1~5시간 추출한 후, 여과 및 건조하는 단계Adding alcohol or an aqueous alcohol solution to the pulverized mixture, extracting at 20-40 ° C for 1-5 hours, filtering and drying

상기 분쇄된 혼합물에 2~3부피배의 알코올 또는 알코올 수용액을 가하고, 20~40℃에서 1~5시간 추출한다. 다음으로, 이를 여과 및 건조하여 분말 형태로 가공한다. 이때, 상기 여과 및 건조방법을 제한하지 않음은 당연하다. 이때, 상기 알코올로는 에탄올을 사용함이 바람직하며, 상기 알코올 수용액의 농도는 제한하지 않는다.2 to 3 times the volume of alcohol or an aqueous alcohol solution is added to the pulverized mixture, and extraction is performed at 20 to 40° C. for 1 to 5 hours. Next, it is filtered and dried to be processed into a powder form. At this time, it is natural that the filtration and drying method is not limited. At this time, it is preferable to use ethanol as the alcohol, and the concentration of the alcohol aqueous solution is not limited.

상기와 같이 제조된 식품첨가물용 조성물은, 앞서 설명된 바와 같이, 식품에 첨가되어 섭취됨으로써, 우수한 관능성을 보이는 것은 물론, 항염 및 항산화 활성이 우수하고, 해독작용이 우수하며, 식품의 보존성을 향상시킨다는 효과가 있다.As described above, the composition for food additives prepared as described above, by being added to food and ingested, exhibits excellent sensory properties, as well as excellent anti-inflammatory and antioxidant activities, excellent detoxification, and preservation of food. has the effect of enhancing.

한편, 상기 건조된 건조물과 말라바시금치를 혼합하는 단계에서, 원추리의 꽃봉오리를 추가로 혼합할 수 있다. On the other hand, in the step of mixing the dried product and Malabar spinach, the flower buds of daylily may be further mixed.

상기 원추리의 꽃봉오리 역시 식품의 맛을 좋게 하고, 항산화 및 항염 활성을 돕는다. 이때, 상기 원추리의 꽃봉오리는 건조 상태의 것을 사용함이 바람직하며, 상기 건조물, 즉 건조된 니파야자 꽃대와 꽃봉오리 100중량부에 대하여 10~20중량부로 혼합될 수 있다. The flower buds of the daylily also improve the taste of food and help antioxidant and anti-inflammatory activity. At this time, it is preferable to use the flower buds of the day lilies in a dried state, and 10 to 20 parts by weight may be mixed with respect to 100 parts by weight of the dried material, that is, the dried nipa palm peduncle and flower buds.

상기 원추리(Hemerocallis fulva)는 외떡잎식물 백합목 백합과의 여러해살이풀로, 비타민A, 베타카로틴, 루테인 함량이 풍부하고, 항산화 활성이 우수하다. The day lily (Hemerocallis fulva) is a perennial plant of the monocotyledonous plant Liliaceae, rich in vitamin A, beta-carotene and lutein, and has excellent antioxidant activity.

아울러, 상기 건조된 건조물과 말라바시금치를 혼합하는 단계에서, 곤달비 잎을 추가로 혼합할 수도 있다. 상기 곤달비 잎 역시 식품의 관능적 특성을 개선하고 감칠맛을 부여하며, 항산화 및 항염 활성을 돕는 것은 물론, 해독작용을 돕는다. 이때, 상기 곤달비 잎은 건조 상태의 것을 사용함이 바람직하며, 상기 건조물, 즉 건조된 니파야자 꽃대와 꽃봉오리 100중량부에 대하여 10~20중량부로 혼합될 수 있다. In addition, in the step of mixing the dried dried material and Malabar spinach, gondola leaves may be additionally mixed. The gondola leaves also improve the sensory properties of food, impart umami, help antioxidant and anti-inflammatory activity, as well as help detoxification. At this time, it is preferable to use the dry gondola leaf, and it may be mixed in 10 to 20 parts by weight based on 100 parts by weight of the dried material, that is, the dried nipa palm flower stalk and flower bud.

상기 곤달비(Ligularia stenocephala) 잎은 플라보노이드, 폴리페놀 성분이 풍부하여 항산화 및 항염 활성이 우수하다.The gondola (Ligularia stenocephala) leaves are rich in flavonoids and polyphenols, and thus have excellent antioxidant and anti-inflammatory activities.

아울러, 상기 건조된 건조물과 말라바시금치를 혼합하는 단계에서, 녹차 잎을 추가로 혼합할 수도 있다. 상기 녹차 잎 역시 식품의 관능적 특성을 개선하고 감칠맛을 부여하며, 항산화 활성 및 항균 활성을 돕는다. 이때, 상기 녹차 잎은 건조 상태의 것을 사용함이 바람직하며, 상기 건조물, 즉 건조된 니파야자 꽃대와 꽃봉오리 100중량부에 대하여 10~20중량부로 혼합될 수 있다. In addition, in the step of mixing the dried product and Malabar spinach, green tea leaves may be additionally mixed. The green tea leaves also improve the sensory properties of food, impart umami, and help antioxidant activity and antibacterial activity. At this time, the green tea leaves are preferably used in a dry state, and may be mixed in an amount of 10 to 20 parts by weight with respect to 100 parts by weight of the dried material, that is, the dried Nipa palm peduncle and flower buds.

상기 녹차 잎은 플라보노이드, 폴리페놀 성분이 풍부하여 항산화 활성이 우수하다.The green tea leaves are rich in flavonoids and polyphenols, and thus have excellent antioxidant activity.

또한, 상기 세척된 꽃대와 꽃봉오리를 끓는 물에 10~40분간 데치는 단계 후, 상기 데친 꽃대와 꽃봉오리에 탱자 효소를 첨가하고 1~5일간 1~5℃에서 숙성하는 단계를 더 포함할 수도 있다.In addition, after the step of blanching the washed peduncle and bud in boiling water for 10 to 40 minutes, adding tangerine enzyme to the blanched peduncle and bud and aging at 1 to 5 ° C for 1 to 5 days. there is.

상기 탱자 효소를 첨가하여 숙성하면, 그 기능성이 더욱 증대되며, 꽃대와 꽃봉오리 특유의 이취가 없어 식품의 풍미를 더욱 향상시킬 수 있다는 장점이 있다. 이때, 그 혼합비는, 상기 데친 꽃대와 꽃봉오리 대 탱자 효소를 1:0.001~0.01 중량비로 첨가함이 바람직하다. When the Tangja enzyme is added and matured, its functionality is further increased, and there is an advantage in that the flavor of the food can be further improved because there is no peculiar smell of flower stems and flower buds. At this time, the mixing ratio is preferably added in a weight ratio of 1: 0.001 to 0.01 for the blanched flower stem and flower bud to Tangja enzyme.

본 발명에서 상기 탱자 효소는 탱자의 열매에 설탕을 1:2~10 중량비로 첨가하여 10~25℃에서 10~100일 동안 숙성하고 여과하여 제조된 것을 사용한다. 이때, 상기 설탕으로는 유기농 원당, 황설탕, 백설탕, 흑설탕 등을 모두 이용할 수 있는 것으로, 그 종류를 제한하지 않는다. 아울러, 상기 여과방법은 면포 등을 이용하면 족한 것으로, 그 방법을 제한하지 않는다.In the present invention, the Tangja enzyme is prepared by adding sugar to the fruit of Tangja at a weight ratio of 1:2 to 10, aging at 10 to 25 ° C. for 10 to 100 days, and filtering. At this time, organic raw sugar, brown sugar, white sugar, brown sugar, etc. can all be used as the sugar, and the type is not limited. In addition, the filtration method is sufficient to use a cotton cloth or the like, and the method is not limited.

따라서, 상기와 같이 제조되는 본 발명의 식품첨가물용 조성물에 따르면, 식품의 기능성, 즉 항산화 및 항염, 그리고 항균 활성 및 해독작용을 높여주어 소비자의 건강유지를 도모하며, 감칠맛 역시 부여하여 관능성을 개선하는 것이다. 또한, 항염 및 항균 활성을 통해 식료의 보존성 역시 좋게 하는 효과도 있다. Therefore, according to the composition for food additives of the present invention prepared as described above, the functionality of food, that is, antioxidant and anti-inflammatory, and antibacterial activity and detoxification are increased to promote the maintenance of health of consumers, and savory taste is also given to increase the sensory is to improve In addition, there is also an effect of improving the preservation of food through anti-inflammatory and antibacterial activity.

이하, 본 발명을 구체적인 실시예를 통해 더욱 상세히 설명한다.Hereinafter, the present invention will be described in more detail through specific examples.

(실시예 1) : NFE(Nypa Fruticans Ethanol) 1(Example 1): NFE (Nypa Fruticans Ethanol) 1

먼저, 니파야자 꽃대와 꽃봉오리 채취하고 물로 깨끗이 세척한 후, 이를 100℃의 끓는 물에 20분간 데치고, 상기 데친 꽃대와 꽃봉오리를 찬물에 담가 식혔다. 다음으로, 상기 데친 꽃대를 길이방향으로 길게 찢어 데친 꽃봉오리와 함께 60℃의 열풍건조기를 이용하여 수분함량이 7%가 되도록 건조하였다.First, the flower stems and flower buds of nipa palm were collected, washed thoroughly with water, blanched in boiling water at 100 ° C. for 20 minutes, and the blanched flower stems and flower buds were immersed in cold water to cool. Next, the blanched peduncles were torn lengthwise and dried together with the blanched flower buds to a moisture content of 7% using a hot air dryer at 60 ° C.

그리고 말라바시금치의 잎을 50℃의 열풍건조기에서 건조시켜 수분 함량이 5%가 되도록 한 후, 그 건조된 잎 300g을 상기 건조된 니파야자 꽃대 700g 및 꽃봉오리 300g과 혼합하였다. In addition, the leaves of Malabar spinach were dried in a hot air dryer at 50 ° C. to have a moisture content of 5%, and then 300 g of the dried leaves were mixed with 700 g of the dried nipa palm flower stalks and 300 g of flower buds.

다음으로, 상기 혼합된 혼합물을 130℃에서 5분간 로스팅하였다. 로스팅 시에는 밀폐된 로스팅기를 이용하였다. 그리고 상기 로스팅한 혼합물을 300mesh로 분쇄한 후, 이에 2부피배의 50% 알코올 수용액을 가하고 25℃에서 5시간 추출하고, 여과 및 동결건조하였다.Next, the mixed mixture was roasted at 130° C. for 5 minutes. For roasting, a closed roasting machine was used. Then, after pulverizing the roasted mixture with 300 mesh, a 2-fold volume of 50% alcohol aqueous solution was added thereto, followed by extraction at 25° C. for 5 hours, followed by filtration and freeze-drying.

(실시예 2) : NFE 2(Example 2): NFE 2

실시예 1과 동일하게 실시하되, 상기 말라바시금치를 혼합할 시, 건조된 원추리의 꽃봉오리 100g 및 건조된 곤달비 잎 100g을 추가로 혼합하였다.It was carried out in the same manner as in Example 1, but when mixing the Malabar spinach, 100 g of dried flower buds and 100 g of dried gondola leaves were additionally mixed.

(실시예 3) : NFE 3(Example 3): NFE 3

실시예 1과 동일하게 실시하되, 상기 데친 꽃대와 꽃봉오리에 탱자 효소를 1:0.005 중량비로 첨가하고, 5℃에서 2일간 숙성하였다.It was carried out in the same manner as in Example 1, but Tangja enzyme was added to the blanched peduncles and buds at a weight ratio of 1:0.005, and aged at 5 ° C for 2 days.

상기 탱자 효소는 세척된 탱자에 열매에 설탕을 1:5 중량비로 첨가하여 22℃에서 20일 동안 숙성하고 여과하여 제조하였다.The Tangja enzyme was prepared by adding sugar to fruits in a weight ratio of 1:5 to washed Tangja, aging at 22° C. for 20 days, and filtering.

(실시예 4) : NFE 4(Example 4): NFE 4

실시예 1과 동일하게 실시하되, 상기 말라바시금치를 혼합할 시, 건조된 녹차 잎 100g을 추가로 혼합하였다.It was carried out in the same manner as in Example 1, but when mixing the Malabar spinach, 100 g of dried green tea leaves were additionally mixed.

(비교예 1)(Comparative Example 1)

실시예 1과 동일하게 실시하되, 말라바시금치를 사용하지 않았다.It was carried out in the same way as in Example 1, but Malabar spinach was not used.

(시험예 1)(Test Example 1)

항산화 활성 측정Antioxidant activity measurement

(1) DPPH radical 소거능 측정(1) Measurement of DPPH radical scavenging ability

DDPH(1,1-diphenyl-2-picryhydrazyl, Sigma-Aldrich Co.)를 이용하여 시료의 라디칼 소거효과를 보는 Biois법을 활용하였다. 각 시료를 농도별로 제조한 시료 용액 1ml에 0.2mM DPPH 1ml를 넣고, vortex mixer를 사용하여 혼합하고 암실에서 30분간 반응시킨 후 517nm에서 흡광도를 측정하였다. 그리고 아래의 식을 이용하여 free radical scavenging activity 곡선을 작성한 후, IC50을 산출하였으며, 양성대조군으로 ascorbic acid를 사용하였다. The Biois method was used to see the radical scavenging effect of the sample using DDPH (1,1-diphenyl-2-picryhydrazyl, Sigma-Aldrich Co.). 1ml of 0.2mM DPPH was added to 1ml of each sample solution prepared by concentration, mixed using a vortex mixer, reacted for 30 minutes in a dark room, and absorbance was measured at 517nm. And after drawing the free radical scavenging activity curve using the formula below, IC 50 was calculated, and ascorbic acid was used as a positive control.

Radical scavenging activity(%)={(control Absorbance - Sample Absorbance)/control Absorbance}×100Radical scavenging activity (%)={(control Absorbance - Sample Absorbance)/control Absorbance}×100

DPPH radical 소거능 측정 결과DPPH radical scavenging activity measurement result 구분division IC50(ug/ml)IC 50 (ug/ml) NFE 1NFE 1 6.00±1.546.00±1.54 NFE 2NFE 2 5.77±0.795.77±0.79 NFE 3NFE 3 5.66±0.895.66±0.89 NFE 4NFE 4 5.60±1.005.60±1.00 비교예 1Comparative Example 1 8.81±2.158.81±2.15

IC50 값은 추출물을 첨가하지 않은 대조군의 값을 50% 감소시키는 추출물의 농도를 나타낸 것으로, IC50 값이 작을수록 항산화 활성이 높은 것을 뜻한다. 따라서 상기 표 1에서와 같이, NFE 1 내지 4의 항산화능은 비교예 1에 비하여 현저히 우수함을 확인할 수 있었다.The IC 50 value represents the concentration of the extract that reduces the value of the control group to which the extract was not added by 50%, and the smaller the IC 50 value, the higher the antioxidant activity. Therefore, as shown in Table 1 above, it was confirmed that the antioxidant capacity of NFEs 1 to 4 was significantly superior to that of Comparative Example 1.

(2) ABTS radical 소거능 측정(2) Measurement of ABTS radical scavenging ability

ABTS radical 소거능 측정은 Pellegrin 등의 방법으로 측정하였다. 7mM ABTS(2,2'-azinobis(3-ethylenbenzothiazoline-6-sulfonate), Sigma-Aldrich Co.) 5ml와 140mM K2S2O8(Sigma-Aldrich Co.) 88㎕를 잘 섞어 16시간 이상 암소에 방치시켰다.ABTS radical scavenging activity was measured by the method of Pellegrin et al. 5 ml of 7 mM ABTS (2,2'-azinobis (3-ethylenbenzothiazoline-6-sulfonate), Sigma-Aldrich Co.) and 88 μl of 140 mM K2S2O8 (Sigma-Aldrich Co.) were mixed well and left in the dark for 16 hours or more.

이를 absolute ethanol과 1:88 비율로 섞어 734nm에서 대조구의 흡광도 값이 0.7±0.002가 되도록 조절한 ABTS solution을 만들었다. 시료 용액 50㎕에 ABTS solution 1ml를 넣어 혼합하고, 2.5분간 암실에서 방치한 후, 734nm에서 흡광도를 측정하였다. 그리고 IC50을 산출하였으며, 그 결과는 하기 표 2에 나타내었다.This was mixed with absolute ethanol at a ratio of 1:88 to make an ABTS solution adjusted so that the absorbance value of the control at 734 nm was 0.7 ± 0.002. 50 μl of the sample solution was mixed with 1 ml of ABTS solution, left in the dark for 2.5 minutes, and absorbance was measured at 734 nm. And IC 50 was calculated, and the results are shown in Table 2 below.

ABTS radical 소거능 측정 결과ABTS radical scavenging activity measurement result 구분division IC50(ug/ml)IC 50 (ug/ml) NFE 1NFE 1 11.21±3.0211.21±3.02 NFE 2NFE 2 10.49±2.9810.49±2.98 NFE 3NFE 3 10.41±2.2510.41±2.25 NFE 4NFE 4 11.04±2.3911.04±2.39 비교예 1Comparative Example 1 14.57±3.4114.57±3.41

상기 표 2에서와 같이, NFE 1 내지 4의 항산화능은 비교예 1에 비하여 현저히 우수함을 확인할 수 있었다.As shown in Table 2, it was confirmed that the antioxidant capacity of NFEs 1 to 4 was significantly superior to that of Comparative Example 1.

(3) ORAC(Oxygen radical absorbance capacity) value 측정(3) ORAC (Oxygen radical absorbance capacity) value measurement

퍼옥시 라디칼 소거능을 통한 항산화 활성 측정은 peroxyl radical scavenging capacity(ORACROO·) 분석법을 이용하였다. 농도별 시료에 peroxyl radical 생성을 위한 AAPH를 최종 반응 농도가 20M이 되도록 처리하고, 형광표준 용액인 fluorescein를 최종 반응 농도가 40nM이 되도록 처리하고, 최종 반응 농도 1 μM의 Trolox를 control standard로 사용였다. Peroxyl radical의 감소를 나타내는 형광감소율은 GENiosfluorescence plate reader(Tecan Trading AG, Salzburg, Austria)를 이용하여 excitation wavelength 485nm, emission wavelength 535nm에서 매 1분마다 측정하였다. 최종결과는 시료의 형광값과 공시험값의 형광값 간의 넓이 차이로 계산하였다. 그 값은 ORAC value per 1ug으로 하기 표 3에 나타내었다.The antioxidant activity was measured through the peroxy radical scavenging ability using the peroxyl radical scavenging capacity (ORACROO·) assay. Samples for each concentration were treated with AAPH for peroxyl radical generation at a final reaction concentration of 20 M, and fluorescein, a fluorescence standard solution, at a final reaction concentration of 40 nM, and Trolox at a final reaction concentration of 1 μM was used as a control standard. . The fluorescence reduction rate representing the reduction of peroxyl radical was measured every minute at an excitation wavelength of 485 nm and an emission wavelength of 535 nm using a GENiosfluorescence plate reader (Tecan Trading AG, Salzburg, Austria). The final result was calculated as the difference in area between the fluorescence value of the sample and the fluorescence value of the blank test value. The values are shown in Table 3 below as ORAC values per 1 ug.

ORAC value 측정 결과 ORAC value measurement result 구분division ORAC value per 1ugORAC value per 1ug NFE 1NFE 1 56.58±3.3656.58±3.36 NFE 2NFE 2 60.11±3.2460.11±3.24 NFE 3NFE 3 57.61±3.5757.61±3.57 NFE 4NFE 4 56.68±3.3856.68±3.38 비교예 1Comparative Example 1 48.43±3.4548.43±3.45

상기 표 3에서와 같이, NFE 1 내지 4의 ORAC value는 비교예 1에 비해 현저히 우수함을 확인할 수 있었다.As shown in Table 3, it was confirmed that the ORAC values of NFEs 1 to 4 were significantly superior to those of Comparative Example 1.

(시험예 2)(Test Example 2)

항염 활성 측정Anti-inflammatory activity measurement

항염증 효과의 염증성 인자로는 Nitrite(혹은 일산화 산소), IL-1β(인터루킨-1베타, interleukin-1beta)를 사용하였다. 시험방법은, 대식세포(macrophage)에 LPS(lipopolysaccharide)를 이용하여 염증을 유발하고, 여기에 시료를 처리하였을 때의 일반적인 염증 지표들이 억제되었는지를 조사하였다. 양성 대조군으로서는 DEX(Dexamethasone)을 비교약물로 사용하였다. 상기 시료는 NFE 1의 시료를 농도에 맞게 희석하여 사용하였다.Nitrite (or oxygen monoxide) and IL-1β (interleukin-1beta) were used as inflammatory factors with anti-inflammatory effects. In the test method, inflammation was induced using lipopolysaccharide (LPS) in macrophages, and whether general inflammatory indicators were suppressed when the sample was treated thereto was investigated. As a positive control, DEX (Dexamethasone) was used as a comparative drug. The sample was used by diluting the sample of NFE 1 according to the concentration.

그리고 그 결과를 도 2 및 도 3에 나타내었다.And the results are shown in Figures 2 and 3.

도 2에서 확인할 수 있는 바와 같이, LPS로 염증 유도시 Nitrite가 크게 증가하였으나, 시료의 처리로 인하여 증가했던 Nitrite가 감소함을 확인할 수 있었다. 또한, 도 3에서 확인할 수 있는 바와 같이, LPS로 인한 염증 유도시 cytokine이 크게 증가하였으나, 시료의 처리로 그 염증이 완화되는 것을 확인하였다. As can be seen in Figure 2, it was confirmed that nitrite increased greatly when inflammation was induced by LPS, but increased nitrite decreased due to treatment of the sample. In addition, as can be seen in Figure 3, it was confirmed that the cytokine greatly increased when inflammation was induced by LPS, but the inflammation was alleviated by the treatment of the sample.

따라서, 본 발명에 의한 조성물은 농도 의존적으로 염증성 인자들의 억제효과가 있으며, 항염증 성분으로 알려진 DEX와 유사한 수준의 억제 효과가 있을 것을 판단되었다. Therefore, it was determined that the composition according to the present invention has an inhibitory effect on inflammatory factors in a concentration-dependent manner and has an inhibitory effect similar to that of DEX, which is known as an anti-inflammatory component.

(시험예 3)(Test Example 3)

글루타티온-S-트랜스퍼레이스와 퀴논 리덕타아제의 활성 측정Measurement of activity of glutathione-S-transferase and quinone reductase

시료의 해독작용을 글루타티온-S-트랜스퍼레이스와 퀴논 리덕타아제의 활성 증가로 측정하였다. 상기 글루타티온-S-트랜스퍼레이스(GST)와 퀴논 리덕타아제(QR)는 무독화 효소 중 대표적인 효소라 할 수 있다.Detoxification of the sample was measured by increasing the activities of glutathione-S-transferase and quinone reductase. The glutathione-S-transferase (GST) and quinone reductase (QR) can be said to be representative enzymes among detoxifying enzymes.

10%의 소태아혈청 및 1% 페니실린-스트랩토마이신이 함유된 둘베코개량 이글배지에 Hepa1c1c7 세포(ATCC CRL-2026)를 4×105개/㎖가 되도록 현탁하고, 상기 세포 현탁액을 96-well 플레이트의 각 웰에 0.2㎖씩 첨가한 뒤, 5% CO2 인큐베이터에서 37℃로 24시간 동안 배양하였다. 그 다음 새 둘베코개량 이글배지로 교환한 뒤, 한 세트의 대조군에는 증류수를, 다른 세트들에는 각각의 시료를 50㎍/㎖ 양으로 첨가하고 24시간 동안 배양하였다. 배양종료 후 배지를 제거하고, 인산완충염 용액으로 세포를 세정하며, 0.1㎖의 세포 용해액을 첨가한 뒤 37℃에서 10분간 인큐베이션했다.Hepa1c1c7 cells (ATCC CRL-2026) were suspended in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum and 1% penicillin-streptomycin at a concentration of 4 × 10 5 cells/ml, and the cell suspension was 96- After adding 0.2 ml to each well of the well plate, it was cultured for 24 hours at 37° C. in a 5% CO 2 incubator. Then, after replacing with a new Dulbecco's modified Eagle's medium, distilled water was added to one set of control groups, and each sample was added in an amount of 50 μg/ml to the other sets and cultured for 24 hours. After completion of the culture, the medium was removed, the cells were washed with a phosphate buffered saline solution, and 0.1 ml of cell lysate was added, followed by incubation at 37°C for 10 minutes.

GST 활성의 측정을 위하여, 각군의 세포액 25㎕에 155㎕의 반응용액(인산칼륨완충액의 13mM 글루타티온)을 첨가하였으며, 측정 전에 20㎕의 반응기질 10mM CDNB(2,4-디니트로클로로벤젠)을 첨가하고, 340nm 파장에서의 흡광도 변화를 측정하였다. 각 활성 분석은 3회 반복 측정하였으며, 단백량은 샘플용액을 인산완충염 용액으로 50배 희석하고, MicroBCA 단백질 분석키트를 사용하여 측정하였다.For the measurement of GST activity, 155 μl of the reaction solution (13 mM glutathione in potassium phosphate buffer) was added to 25 μl of the cell fluid of each group, and 20 μl of 10 mM CDNB (2,4-dinitrochlorobenzene) as a reactive substrate was added before measurement. was added, and the change in absorbance at a wavelength of 340 nm was measured. Each activity assay was repeatedly measured three times, and the amount of protein was measured by diluting the sample solution 50 times with a phosphate buffered salt solution and using a MicroBCA protein assay kit.

각 GST의 활성은 대조군의 GST 값에 대한 상대 값으로 아래의 식에 의해 산출하였고, 그 결과는 하기 표 4에 나타내었다.The activity of each GST was calculated by the formula below as a relative value to the GST value of the control group, and the results are shown in Table 4 below.

GST 활성 = (시험 물질 첨가 섹션의 최대 레이트 계수/시험 물질 첨가 섹션의 단백량) / (물 첨가 섹션의 최대레이트 계수/물 첨가 섹션의 단백량)GST activity = (maximum rate factor in test substance addition section/protein amount in test substance addition section)/(maximum rate factor in water addition section/protein amount in water addition section)

QR 활성의 측정을 위하여, 각군의 세포액 25㎕에 100㎕의 반응용액을 첨가하였다. 이때 기질 존재 및 부재를 설정하고, 기질 존재에는 반응용액에 또한 최종 농도 50μM이 되도록 메나디온을 첨가하였다. 활성분석은 각각 3회 반복 실시하였다. 또한, QR 활성은 대조군에 대한 QR 상대 활성으로서 아래식에 의해 산출하였고, 그 결과는 하기 표 4에 나타내었다.For measurement of QR activity, 100 μl of the reaction solution was added to 25 μl of the cell solution of each group. At this time, the presence and absence of the substrate were set, and menadione was added to the reaction solution to a final concentration of 50 µM in the presence of the substrate. Activity assays were repeated three times, respectively. In addition, QR activity was calculated by the formula below as QR relative activity to the control group, and the results are shown in Table 4 below.

QR 활성= {[(시험 물질 첨가 섹션의 기질 존재의 흡광도) - (시험 물질 첨가 섹션의 기질 부재의 흡광도)] ÷ (시험 물질 첨가 센션의 단백량)} ÷ {[물 첨가 섹션의 기질 존재의 흡광도] - (물 첨가 섹션의 기질 없음의 흡광도)} ÷ (물 첨가 섹션의 단백량)}QR activity = {[(absorbance of substrate present in test substance addition section) - (absorbance of substrate absence in test substance addition section)] ÷ (protein amount of test substance addition section)} ÷ {[absorbance of substrate present in water addition section ] - (absorbance without substrate in water added section)} ÷ (protein amount in water added section)}

시험예 3의 결과.Results of Test Example 3. 구분division NFE 1NFE 1 NFE 2NFE 2 NFE 3NFE 3 NFE 4NFE 4 비교예 1Comparative Example 1 GST 활성GST active 1.551.55 1.591.59 1.561.56 1.551.55 1.281.28 QR 활성QR active 1.731.73 1.801.80 1.741.74 1.731.73 1.411.41

상기 표4에서 확인할 수 있는 바와 같이, 본 발명의 NFE 1 내지 4는 GST 효소 및 QR 효소의 활성 증가 효과가 우수함을 확인할 수 있었다.As can be seen in Table 4, it was confirmed that the NFEs 1 to 4 of the present invention were excellent in increasing the activity of the GST enzyme and the QR enzyme.

(시험예 4)(Test Example 4)

훈련된 10명의 관능요원에게 시료를 평가하게 하였다. 평가항목은 외관, 맛, 향, 전체적인 기호도였고, 평가는 9점 척도법을 사용하였으며, 9에 가까울수록 극도로 좋고, 1에 가까울수록 극도로 싫은 것으로 나타내었다.Samples were evaluated by 10 trained sensory personnel. The evaluation items were appearance, taste, aroma, and overall acceptability, and the evaluation was performed using a 9-point scale.

시료로는 시판되는 오렌지주스, 우유를 이용하였는바, 먼저, 오렌지주스 200ml에 상기 NFE 1 내지 4, 비교예 1을 각각 10g씩 첨가, 혼합하여 사용하였다. 그리고 우유 200ml에도 상기 NFE 1 내지 4, 비교예 1을 각각 10g씩 첨가, 혼합하여 사용하였다. As samples, commercially available orange juice and milk were used. First, 10 g of NFEs 1 to 4 and Comparative Example 1 were added to 200 ml of orange juice and mixed. In addition, 10 g of each of NFEs 1 to 4 and Comparative Example 1 were added to 200 ml of milk, and mixed and used.

상기 관능평가의 결과는 하기 표 5에 나타냈다.The results of the sensory evaluation are shown in Table 5 below.

시험예 4의 결과.Results of Test Example 4. 구분division 외관Exterior taste incense 기호도preference
오렌지주스

Orange juice
NFE 1NFE 1 5.25.2 6.16.1 5.55.5 5.65.6
NFE 2NFE 2 5.25.2 6.56.5 5.85.8 5.95.9 NFE 3NFE 3 5.25.2 7.07.0 6.16.1 6.46.4 NFE 4NFE 4 5.25.2 6.16.1 5.65.6 5.65.6 비교예 1Comparative Example 1 5.35.3 4.04.0 4.24.2 4.24.2 대조군(무첨가)Control (no additives) 5.05.0 5.05.0 5.05.0 5.05.0
우유


milk

NFE 1NFE 1 5.05.0 5.75.7 5.85.8 5.75.7
NFE 2NFE 2 5.15.1 6.36.3 6.46.4 6.26.2 NFE 3NFE 3 5.15.1 6.56.5 6.66.6 6.56.5 NFE 4NFE 4 5.15.1 5.75.7 5.15.1 5.75.7 비교예 1Comparative Example 1 5.05.0 4.54.5 4.74.7 4.64.6 대조군(무첨가)Control (no additives) 5.05.0 5.05.0 5.05.0 5.05.0

상기 표 5에서 확인할 수 있는 바와 같이, 본 발명에 의한 NFE 1 내지 4는 비교예 1 및 대조군과 비교할 때, 오렌지주스, 우유 모두 맛, 향, 기호도에 있어서 더 우수한 평가를 받았음을 확인하였다. 다만, 외관에 있어서는 큰 차이는 없었다.As can be seen in Table 5, it was confirmed that NFEs 1 to 4 according to the present invention received superior evaluation in taste, aroma, and acceptability for both orange juice and milk compared to Comparative Example 1 and the control group. However, there was no significant difference in appearance.

이상, 본 발명을 바람직한 실시예를 사용하여 상세히 설명하였으나, 본 발명의 범위는 특정 실시예에 한정되는 것은 아니며, 첨부된 특허청구범위에 의하여 해석되어야 할 것이다. 또한, 이 기술분야에서 통상의 지식을 습득한 자라면, 본 발명의 범위에서 벗어나지 않으면서도 많은 수정과 변형이 가능함을 이해하여야 할 것이다.In the above, the present invention has been described in detail using preferred embodiments, but the scope of the present invention is not limited to specific embodiments, and should be interpreted according to the appended claims. In addition, those skilled in the art should understand that many modifications and variations are possible without departing from the scope of the present invention.

Claims (6)

니파야자 꽃대와 꽃봉오리를 세척하는 단계와,
상기 세척된 꽃대와 꽃봉오리를 끓는 물에 10~40분간 데치는 단계와,
상기 데친 꽃대와 꽃봉오리를 건조하는 단계와,
상기 건조된 건조물과 말라바시금치를 혼합하는 단계와,
상기 혼합된 혼합물을 로스팅하는 단계와,
상기 로스팅된 혼합물을 분쇄하는 단계와,
상기 분쇄된 혼합물에 알코올 또는 알코올 수용액을 가하고 20~40℃에서 1~5시간 추출한 후, 여과 및 건조하는 단계를 포함하는 것을 특징으로 하는 니파야자 꽃대 및 꽃봉오리의 알코올추출물을 이용한 식품첨가물용 조성물의 제조방법.
Washing the flower stalks and flower buds of nipa palm;
Blanching the washed flower stalks and flower buds in boiling water for 10 to 40 minutes;
Drying the blanched peduncles and buds;
Mixing the dried product with Malabar spinach;
roasting the mixed mixture;
Grinding the roasted mixture;
Adding alcohol or an aqueous alcohol solution to the pulverized mixture, extracting at 20-40 ° C. for 1-5 hours, filtering and drying A composition for food additives using an alcohol extract of flower stalks and flower buds of nipa palm Manufacturing method of.
제1항에 있어서,
상기 건조된 건조물과 말라바시금치를 혼합하는 단계에서,
원추리의 꽃봉오리를 추가로 혼합하는 것을 특징으로 하는 니파야자 꽃대 및 꽃봉오리의 알코올추출물을 이용한 식품첨가물용 조성물의 제조방법.
According to claim 1,
In the step of mixing the dried dried product and Malabar spinach,
A method for producing a composition for food additives using an alcohol extract of flower stalks and flower buds of nipa palm, characterized in that the flower buds of daylily are additionally mixed.
제1항 또는 제2항에 있어서,
상기 건조된 건조물과 말라바시금치를 혼합하는 단계에서,
곤달비 잎을 추가로 혼합하는 것을 특징으로 하는 니파야자 꽃대 및 꽃봉오리의 알코올추출물을 이용한 식품첨가물용 조성물의 제조방법.
According to claim 1 or 2,
In the step of mixing the dried dried product and Malabar spinach,
Method for producing a composition for food additives using an alcohol extract of nipa palm flower stalks and flower buds, characterized in that the Gondalbi leaves are additionally mixed.
제1항에 있어서,
상기 세척된 꽃대와 꽃봉오리를 끓는 물에 10~40분간 데치는 단계 후,
상기 데친 꽃대와 꽃봉오리에 탱자 효소를 첨가하고 1~5일간 1~5℃에서 숙성하는 단계를 더 포함하는 것을 특징으로 하는 니파야자 꽃대 및 꽃봉오리의 알코올추출물을 이용한 식품첨가물용 조성물의 제조방법.
According to claim 1,
After the step of blanching the washed peduncles and flower buds in boiling water for 10 to 40 minutes,
Method for producing a composition for food additives using an alcohol extract of Nipa palm flower stalks and buds, characterized in that it further comprises the step of adding tangerine enzyme to the blanched flower stalks and buds and aging at 1 to 5 ° C for 1 to 5 days .
제4항에 있어서,
상기 탱자 효소는,
탱자에 열매에 설탕을 1:2~10 중량비로 첨가하여 10~25℃에서 10~100일 동안 숙성하고 여과하여 제조된 것임을 특징으로 하는 니파야자 꽃대 및 꽃봉오리의 알코올추출물을 이용한 식품첨가물용 조성물의 제조방법.
According to claim 4,
The Tangja enzyme,
A composition for food additives using alcohol extracts of flower stalks and flower buds of nipa palm, characterized in that it is prepared by adding sugar to tangerine fruits in a weight ratio of 1:2 to 10, aging at 10 to 25 ° C for 10 to 100 days, and filtering Manufacturing method of.
제1항에 있어서,
상기 건조된 건조물과 말라바시금치를 혼합하는 단계에서,
녹차 잎을 추가로 혼합하는 것을 특징으로 하는 니파야자 꽃대 및 꽃봉오리의 알코올추출물을 이용한 식품첨가물용 조성물의 제조방법.
According to claim 1,
In the step of mixing the dried dried product and Malabar spinach,
A method for producing a composition for food additives using an alcohol extract of nipa palm flower stalks and flower buds, characterized in that green tea leaves are additionally mixed.
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KR101710194B1 (en) 2016-07-12 2017-02-27 주식회사 토디팜코리아 Liquefied composition with nipa fruticans wurmb
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KR101710194B1 (en) 2016-07-12 2017-02-27 주식회사 토디팜코리아 Liquefied composition with nipa fruticans wurmb
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