KR20230102319A - A composition for detecting microorganisms - Google Patents
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Abstract
본 발명은 배양보틀 내에 존재하는 미생물을 검출하기 위한 조성물로서, 상기 조성물은 상기의 화학식 1로 표현되는 화합물과 유니버셜 인디케이터 및 실리콘 조색제를 유효성분으로 포함하여 형성되고, 본 발명의 조성물은 배양배지 내에 존재하는 미생물의 개체수가 증가함에 따라 발생되는 이산화탄소에 의해서 배양배지가 산성으로 변화할 경우에 발생하는 조성물의 색변화를 측정하는 것을 원리로 하고 있으며, 본 발명에서 제공하는 조성물은 종래의 기술에 비하여 적은 양으로도 미생물을 정확하게 검출할 수 있어서, 미생물 검출에 소요되는 시간과 비용을 절감할 수 있다.The present invention is a composition for detecting microorganisms present in a culture bottle, the composition comprising the compound represented by Chemical Formula 1, a universal indicator, and a silicone colorant as active ingredients, and the composition of the present invention is contained in a culture medium The principle is to measure the color change of the composition that occurs when the culture medium is changed to acidity by carbon dioxide generated as the number of microorganisms present increases, and the composition provided by the present invention is superior to conventional techniques. Microorganisms can be accurately detected even with a small amount, so time and cost required for detecting microorganisms can be reduced.
Description
본 발명은 혈액을 이용하여 질병을 진단하는 과정에서, 혈류 내 미생물로 인한 질병의 진단에 소요되는 시간을 단축시키기 위하여 혈액의 배양단계에서 미리 미생물을 검출할 수 있는 민감도가 높고 정확도가 우수한 형광 센서 조성물에 관한 것이다.In the process of diagnosing diseases using blood, the present invention is a highly sensitive and highly accurate fluorescence sensor capable of detecting microorganisms in advance in the blood culture step in order to shorten the time required for diagnosing diseases caused by microorganisms in the bloodstream. It's about the composition.
최근 코로나 바이러스(COVID 19) 등 신종 바이러스를 비롯한 다양한 균주들의 등장으로 인해 인간의 건강을 위협하고 있다. 하지만 매우 낮은 농도의 균주들은 기하급수적으로 개채수가 증식되기 때문에 신속하게 미생물의 개체수를 파악하는 등 균주의 오염을 방지하기 위한 필요성이 요구되고 있다.Recently, various strains including new viruses such as the corona virus (COVID 19) are threatening human health. However, since strains with very low concentrations proliferate exponentially, there is a need to prevent contamination of strains, such as quickly identifying the population of microorganisms.
바이러스 등에 의한 감염이 예상되는 환자를 조기에 진단하고 적절한 치료를 수행하기 위해서는 혈류 내 미생물의 신속한 검사가 무엇보다도 중요하다.In order to early diagnose a patient who is expected to be infected by a virus or the like and to perform appropriate treatment, rapid examination of microorganisms in the bloodstream is of utmost importance.
상기와 같은 혈류 내 미생물을 검사하기 위해서는 혈액배양이 반드시 필요하고, 배양된 혈액에 대해서 미생물을 진단하였을 경우에 증상이 의심되는 환자의 약 5-15%가 양성으로 진단되므로, 혈액 배양이 양성이라는 이유로 특이적인 항균제로 치료하기에는 부족함이 있다.In order to test microorganisms in the bloodstream as described above, blood culture is absolutely necessary, and when microorganisms are diagnosed for cultured blood, about 5-15% of patients with suspected symptoms are diagnosed as positive, so blood culture is positive. For this reason, there is a lack of treatment with specific antibacterial agents.
따라서 혈액 배양의 목적이 혈류감염의 정확한 진단과 효과적인 항균제를 선택하기 위한 것이라면, 혈액배양 검사의 민감도와 정확도를 향상시켜 증상을 정확하게 판단하고 치료하는 것이 감염자들의 사망률을 낮추는데 반드시 필요하다.Therefore, if the purpose of blood culture is to accurately diagnose bloodstream infections and select effective antibacterial agents, it is essential to improve the sensitivity and accuracy of blood culture tests to accurately determine and treat symptoms to reduce the mortality rate of infected people.
혈류내 미생물 등 균주들의 오염농도를 측정하기 위해서 일반적으로 도말 평판법(spread plate method)을 활용하는데 균주가 포함된 일정량의 액상을 한천배지에 도말하고 균주의 온도 및 습도 등 일정 환경조건을 유지한 상태에서 일정 시간이 지난 후에 균주의 개체수를 측정함으로써 오염농도를 측정하는 방법이지만 고농도의 개체수를 도말할 경우에는 균주의 집락수(colony)를 세기 위해 희석과정을 거쳐야하므로 48시간 혹은 일주일 이상이 소요되는 번거러움이 존재했다.In order to measure the contaminant concentration of strains such as microorganisms in the bloodstream, the spread plate method is generally used. It is a method of measuring the contamination concentration by measuring the population of the strain after a certain period of time has elapsed, but when a high concentration population is smeared, a dilution process is required to count the colony of the strain, which takes 48 hours or more than a week. Trouble existed.
또 다른 방법으로는 혼탁도 측정법(turbidity)으로 분광광도계와 광원을 이용하여 흡광도(optical density, O.D)를 측정하는 방법이 있다. 이러한 혼탁도 측정법은 쉽고 빠르며 시료의 파괴와 훼손이 일어나지 않는 장점을 가지고 있으나, 죽은 개체수가 함께 포함될 수 있기 때문에 정확한 측정법이라고 하기는 어렵다.Another method is a method of measuring optical density (OD) using a spectrophotometer and a light source as a turbidity measurement method. This turbidity measurement method has the advantage that it is easy and fast and does not cause destruction or damage of the sample, but it is difficult to say that it is an accurate measurement method because dead objects may be included together.
액체 시료 속의 세포나 개체수를 측정하는 방법으로 레이저빔, 형광염색약을 이용하여 세포에 레이저광을 쬐여 방사되는 형광과 산란광을 분석하여 농도를 정밀하게 측정하는 유동세포계수기(flow cytometer)이 존재하지만, 이는 정밀한 장비 구성으로 장비 도입 및 측정 시 비용이 많이 요구된다.As a method of measuring the number of cells or populations in a liquid sample, there is a flow cytometer that precisely measures the concentration by exposing the cells to laser light using a laser beam or fluorescent dye and analyzing the emitted fluorescence and scattered light. This is a precise equipment configuration, which requires a lot of cost when introducing and measuring equipment.
본 발명은 상기와 같이 혈류내 미생물 등의 감염진단을 신속하고 정확하게 수행할 수 있는 방법을 제공하고, 이로 인해서 혈류 진단을 위한 장비 등을 최소화 하여 비용을 절감하기 위한 것이다.As described above, the present invention provides a method capable of quickly and accurately diagnosing infection of microorganisms in the bloodstream, thereby reducing costs by minimizing equipment for diagnosing bloodstream.
본 발명은 상기와 같은 문제점을 해결하기 위한 것으로서, 혈액을 배양하는 데에 있어서, 혈류 내에 존재하는 미생물을 신속하게 정확하게 검출하기 위하여 미생물의 배양에 의해 발생되는 이산화탄소에 의해서 배양액의 pH가 변화하는 것을 형광화합물의 색변화를 통해서 신속하게 파악할 수 있는 미생물의 배양을 검출하기 위한 형광 조성물 및 이를 이용한 미생물의 검출방법을 제공하기 위한 것이다.The present invention is to solve the above problems, in culturing blood, in order to quickly and accurately detect microorganisms present in the bloodstream, the pH of the culture medium is changed by carbon dioxide generated by the culture of microorganisms. It is an object of the present invention to provide a fluorescent composition for detecting the culture of microorganisms that can be quickly grasped through a color change of a fluorescent compound and a method for detecting microorganisms using the same.
본 발명에서 제공하는 방법을 통해서 혈류 내에 존재하는 미생물을 신속하게 검출할 수 있고, 종래 기술의 문제점이었던 검출장비가 복잡해지고 비용이 많이 소요된다는 문제점을 해결할 수 있었다. Through the method provided by the present invention, it is possible to quickly detect microorganisms present in the bloodstream, and it is possible to solve the problem that detection equipment, which was a problem in the prior art, is complicated and expensive.
상기와 같은 문제점을 해결하기 위하여 본 발명에서는 하기의 화학식 1로 표현되는 형광 화합물을 포함하는 조성물을 제조하여 혈액배양보틀 내에 존재하여 배양되는 미생물에 대한 특이도와 민감도를 향상시켰다.In order to solve the above problems, in the present invention, a composition containing a fluorescent compound represented by Chemical Formula 1 is prepared to improve specificity and sensitivity to microorganisms present in and cultured in a blood culture bottle.
<화학식 1><Formula 1>
상기 화학식 1에서 R1 및 R2는 각각 독립적으로 H 또는 SO3H 중에서 선택되는 치환기이고, m 및 n은 각각 독립적으로 1 내지 3의 정수이다.In Formula 1, R 1 and R 2 are each independently a substituent selected from H or SO 3 H, and m and n are each independently an integer of 1 to 3.
본 발명에서 제공하는 혈액배양보틀 분석용 조성물은 상기 보틀내에서 배양되는 미생물의 균주수가 증가할수록 미생물이 배출하는 이산화탄소의 양이 증가되고 이로 인해서 배양배지가 산성으로 변환되는 원리는 이용한 것으로서, 본 발명에서 제공하는 형광 조성물은 pH의 변화에 발생되는 형광 화합물의 색변화를 적용한 것으로서, 검출장비를 최소화할 수 있고, 소요되는 비용도 절감할 수 있다.The blood culture bottle analysis composition provided in the present invention uses the principle that as the number of strains of microorganisms cultured in the bottle increases, the amount of carbon dioxide emitted by the microorganisms increases, thereby converting the culture medium to acidity. The fluorescent composition provided by applies the color change of the fluorescent compound generated by the change in pH, and can minimize the detection equipment and reduce the cost required.
본 발명에서 제공하는 혈액배양보틀 분석용 미생물 검출용 조성물은 상기 보틀내에서 배양되는 미생물에 매우 특이적이어서, 종래의 기술보다 적은 양의 조성물로도 미생물을 정확하게 검출할 수 있다. 이에 따라 혈액분석을 위한 검출장비를 최소화할 수 있고 소요되는 비용도 절감할 수 있다.The composition for detecting microorganisms for analysis in a blood culture bottle provided in the present invention is very specific for microorganisms cultured in the bottle, and thus can accurately detect microorganisms even with a smaller amount of the composition than in the prior art. Accordingly, the detection equipment for blood analysis can be minimized and the required cost can be reduced.
도 1은 본 발명에서 제공하는 미생물 검출용 조성물을 제조한 실시예 1 및 2를 나타낸다. A는 실시예 1을 나타내고, B는 실시예 2를 나타낸다.
도 2는 본 발명에서 제공하는 미생물 검출용 조성물을 제조하여 바이알에 주입한 후와 24시간이 경과된 후의 색변화를 나타낸다.
도 3은 본 발명에서 제공하는 미생물 검출용 조성물을 이용하여 호기성 세균 및 혐기성 세균을 검출하는 과정을 나타낸 것이다.
도 4는 종래의 미생물 검출용 조성물을 이용하여 호기성 세균 및 혐기성 세균을 검출하는 과정을 나타낸 것이다.1 shows Examples 1 and 2 in which a composition for detecting microorganisms provided in the present invention was prepared. A represents Example 1 and B represents Example 2.
Figure 2 shows the color change after preparing the composition for detecting microorganisms provided in the present invention and injecting it into a vial and after 24 hours have elapsed.
3 shows a process of detecting aerobic bacteria and anaerobic bacteria using the composition for detecting microorganisms provided in the present invention.
4 shows a process of detecting aerobic bacteria and anaerobic bacteria using a conventional composition for detecting microorganisms.
본 발명은 배양보틀 내에 존재하는 미생물을 검출하기 위한 조성물로서, 본 발명에서 제공하는 상기 조성물은 용매에 유니버설 인디케이터(Universal indicator)와 실리콘 조색제 및 하기의 화학식 1로 표현되는 화합물을 포함하여 형성되는 조성물이다.The present invention is a composition for detecting microorganisms present in a culture bottle, and the composition provided by the present invention is formed by including a universal indicator, a silicone colorant, and a compound represented by Formula 1 in a solvent. am.
<화학식 1><Formula 1>
상기 화학식 1에서 R1 및 R2는 각각 독립적으로 H 또는 SO3H 중에서 선택되는 치환기이고, m 및 n은 각각 독립적으로 1 내지 3의 정수이다.In Formula 1, R 1 and R 2 are each independently a substituent selected from H or SO 3 H, and m and n are each independently an integer of 1 to 3.
상기 실리콘 조색제는 분산용 수지, 분산제, 안료 및 첨가제 등으로 이루어진 조색제로서, 분산용 수지로서는 벤조산을 함유한 것을 첨가하고 분산제로서 폴리아민아미드계 화합물이나 말레인산무수물을 첨가하며 안료로서는 프탈로시아닌계 화합물을 첨가하여 형성된 것이다.The silicone colorant is a colorant composed of a dispersing resin, a dispersing agent, a pigment, and an additive. As the dispersing resin, a benzoic acid-containing resin is added, a polyamineamide-based compound or maleic acid anhydride is added as a dispersing agent, and a phthalocyanine-based compound is added as a pigment. it is formed
구체적으로 본 발명에서 제공하는 미생물 검출용 조성물은 상기 화학식 1로 표현되는 화합물의 중량을 100중량부로 설정했을 경우에, 상기 유니버셜 인디케이터 150 내지 200 중량부, 실리콘 조색제 10 내지 20 중량부를 포함하여 형성된다.Specifically, the composition for detecting microorganisms provided by the present invention includes 150 to 200 parts by weight of the universal indicator and 10 to 20 parts by weight of the silicone colorant when the weight of the compound represented by Formula 1 is set to 100 parts by weight. .
상기 용매로서는 탄소수 1 내지 6의 치환 또는 비치환된 알코올, 디메틸포름알데히드(DMF) 등이 사용될 수 있고, 상기 용매의 첨가량은 구체적으로 한정되지 않는다.As the solvent, a substituted or unsubstituted alcohol having 1 to 6 carbon atoms, dimethylformaldehyde (DMF), and the like may be used, and the addition amount of the solvent is not specifically limited.
본 발명에서 제공하는 상기 미생물 검출용 조성물은 상기 성분 이외에 조성물의 안정성을 위해서 수산화나트륨(NaOH)를 더 첨가할 수 있고, 상기 수산화나트륨의 함량은 상기 화학식 1로 표현되는 화합물의 함량은 100 중량부로 설정하였을 경우에 0.001 내지 0.01 중량부를 첨가할 수 있다.The composition for detecting microorganisms provided in the present invention may further add sodium hydroxide (NaOH) for stability of the composition in addition to the above components, and the content of the sodium hydroxide is 100 parts by weight of the compound represented by Formula 1. When set, 0.001 to 0.01 parts by weight may be added.
본 발명에서 제공하는 미생물 검출용 조성물은 종래의 미생물 검출용 조성물에 비하여 적은 양으로도 미생물의 존재를 정확하게 검출할 수 있다.The composition for detecting microorganisms provided in the present invention can accurately detect the presence of microorganisms even with a small amount compared to conventional compositions for detecting microorganisms.
실시예 1. 미생물 검출 조성물 제조Example 1. Microbial Detection Composition Preparation
유니버셜 인디케이터(Universal indicator, Merck, USA) 25ml를 보틀에 주입한 후에 2 내지 3일 건조하여 용매를 증발시켜 제거한다. 상기 유니버셜 인디케이터는 용액의 pH의 변화에 따라서 색이 변화되는 지식약으로서 당업자에게는 자명한 것이다.After injecting 25 ml of universal indicator (Universal indicator, Merck, USA) into a bottle, it is dried for 2 to 3 days to remove the solvent by evaporation. The universal indicator is a knowledge medicine that changes color according to a change in pH of a solution, and is obvious to those skilled in the art.
상기의 용매가 증발된 유니버셜 인디케이터에 프로판올 3ml를 첨가하여 용해시킨 후에 1.5ml를 취한 후에 10N NaOH 10㎕를 첨가하고 피펫팅하여 혼합한다.After dissolving by adding 3ml of propanol to the universal indicator from which the above solvent was evaporated, 1.5ml was taken and 10μl of 10N NaOH was added and mixed by pipetting.
10g의 GE Silicon RTV 615 component A(General Electric company, USA)와 1.0g의 글리세롤을 디쉬(dish)에 분주한 후에 상기의 유니버설 인디케이터 혼합물 1.5ml를 넣고 천천히 혼합한다. After dispensing 10 g of GE Silicon RTV 615 component A (General Electric company, USA) and 1.0 g of glycerol into a dish, 1.5 ml of the above universal indicator mixture was added and mixed slowly.
상기의 혼합물에 0.15g의 백색 실리콘 조색제와 1.0g의 GE Silicon RTV 615 component B 및 하기의 화학식 1로 표현되는 화합물에서 m=1, n=3, R1=SO3H, R2=SO3H인 화합물 1.0g을 혼합하여 교반한 후에 상기의 혼합물을 주사기를 이용하여 바이알에 적당량 분주하고, 상온에서 24시간 동안 건조시킨 후에 2시간 동안 자외선을 조사하여 멸균시켜, 본 발명의 미생물 검출용 조성물을 제조하였다.In the above mixture, 0.15 g of white silicone colorant, 1.0 g of GE Silicon RTV 615 component B, and a compound represented by Formula 1 below, m = 1, n = 3, R 1 =SO 3 H, R 2 =SO 3 After mixing and stirring 1.0 g of the H compound, the mixture is dispensed in an appropriate amount into a vial using a syringe, dried at room temperature for 24 hours, and then sterilized by irradiation with ultraviolet rays for 2 hours. The composition for detecting microorganisms of the present invention was manufactured.
<화학식 1><Formula 1>
실시예 2. 미생물 검출 조성물 제조Example 2. Microbial Detection Composition Preparation
상기 실시예 1에서 포함된 성분 중에서 10N NaOH 20㎕를 첨가하고, 상기 백색 실리콘 조색제를 0.3g을 첨가하는 것을 제외하고 다른 성분 및 공정은 동일하게 제조하였다.Among the components included in Example 1, 20 μl of 10N NaOH was added and 0.3 g of the white silicone colorant was added, but other components and processes were prepared in the same manner.
상기 실시예 1 및 2의 조성물을 도 1에 제시하였다. 도 1의 A는 상기 실시예 1을 나타내고, B는 실시예 2를 나타낸다.The compositions of Examples 1 and 2 are shown in FIG. 1 . A in FIG. 1 represents Example 1, and B represents Example 2.
상기 실시예 1 및 2에서 제조된 조성물 중 실시예 2로 제조된 조성물은 미생물의 검출시간이 길어질 수 있어서, 아래의 미생물 검출실험에서는 상기 실시예 1의 조성물을 이용하였다.Among the compositions prepared in Examples 1 and 2, the composition prepared in Example 2 may increase the detection time of microorganisms, so the composition of Example 1 was used in the microorganism detection experiment below.
<본 발명의 조성물을 이용한 미생물 검출 실험><Microorganism detection experiment using the composition of the present invention>
본 발명에서 제공하는 미생물 검출용 실리콘 조성물을 이용하여 배양보틀 내에 존재하는 미생물을 검출하기 위하여 상기 실시예 1의 조성물과 종래기술의 조성물을 대비하는 실험을 실시하였다.In order to detect microorganisms present in the culture bottle using the silicone composition for detecting microorganisms provided in the present invention, an experiment was conducted to compare the composition of Example 1 and the prior art composition.
상기의 실험을 실시하기 위한 검출대상 미생물로서는 피부상재균을 사용하였다.Skin flora was used as the microorganism to be detected for carrying out the above experiments.
상기 피부상재균을 준비하는 과정은 다음과 같다.The process of preparing the skin flora is as follows.
먼저 -80℃에 보관되어 있는 균스탁에서 꺼내어 3% Tryptic Soy Broth agar 플레이트에 리스탁한 후에 37℃에서 하루동안 정치배양한다.First, it is taken out of the germ stock stored at -80 ° C, re-stocked on a 3% Tryptic Soy Broth agar plate, and then incubated at 37 ° C for one day.
정치배양된 상기 스탁에서 콜로니 2 내지 3개를 취하여 3% TSB 배양액에 접종하고, 37℃, 230rpm에서 10 내지 12 시간 동안 진탕배양하고, Tryptic Soy Broth로 희석하여 1CFU/ml의 농도로 10ml를 준비한다.2 to 3 colonies were taken from the above statically cultured stock, inoculated into a 3% TSB culture medium, cultured with shaking at 37 ° C and 230 rpm for 10 to 12 hours, and diluted with Tryptic Soy Broth to prepare 10 ml at a concentration of 1 CFU / ml do.
상기 피부상재균을 배양하기 위한 배양액은 아래와 같은 조성으로 보틀에 준비하여 혼합한 후에 121℃에서 15 내지 20분 동안 고압증기로 멸균한다.The culture solution for culturing the skin flora is prepared in a bottle with the following composition, mixed, and then sterilized with high-pressure steam at 121° C. for 15 to 20 minutes.
호기성 배양액aerobic culture
혐기성 배양액anaerobic broth
상기 호기성 및 혐기성 배양액을 각각 제조한 후에 20ml 바이알에 넣고, 실시예 1에서 제조된 미생물 검출용 조성물 1.5ml를 첨가한 후에 24시간이 지난 후의 색변화를 관찰하여 도 2에 제시하였다.After preparing the aerobic and anaerobic cultures, respectively, they were placed in a 20ml vial, and 1.5ml of the composition for detecting microorganisms prepared in Example 1 was added, and the color change after 24 hours was observed and presented in FIG. 2 .
도 2에 의하면, 상기 바이알에 미생물 검출용 조성물을 첨가한 후(도 2의 A)와 24시간이 지난 후(도 2의 B)의 색변화를 대비하면, 24시간 후에 상기 미생물 검출용 조성물에 의해서 색변화가 관찰된 것을 알 수 있다.According to Figure 2, when comparing the color change after adding the composition for detecting microorganisms to the vial (A in Fig. 2) and after 24 hours (B in Fig. 2), the composition for detecting microorganisms after 24 hours It can be seen that a color change was observed.
본 발명에서 제공하는 미생물 검출용 조성물과 종래에서 제공하는 미생물 검출용 조성물의 색변화를 대비하기 위해서, 상기 호기성 및 혐기성 배양액을 각각 제조한 후에 20ml 바이알에 넣고, 상기에서 준비된 피부상재균을 상기 배양액에 주입한 후에, 상기 실시예 1에서 제조된 미생물 검출용 조성물을 1.5ml를 첨가한 후에 상온에서 보관하고, 접종후에 13시간이 지난 후에 1시간 단위로 색변화를 관찰하고 촬영한 결과를 도 3에 제시하였다.In order to compare the color change of the composition for detecting microorganisms provided in the present invention and the composition for detecting microorganisms provided in the prior art, after preparing the aerobic and anaerobic cultures, respectively, put them in a 20ml vial, and the skin flora prepared above were prepared in the culture medium After injection, 1.5 ml of the composition for detecting microorganisms prepared in Example 1 was added and stored at room temperature, and after 13 hours after inoculation, color change was observed and photographed in units of 1 hour. The results were shown in FIG. presented in
종래의 기술과 대비하기 위해서 호기성 세균의 배양액에는 BACT/ALERT FA Plus(BioMerieux, France) 1.5ml를, 혐기성 세균의 배양액에는 BACT/ALERT FN Plus 17(BioMerieux, France) 1.5ml를 상기 바이알에 실시예 1에서 제조된 조성물을 대신하여 각각 주입하고 13시간이 지난 후에 1시간 단위로 색변화를 관찰하고 촬영한 결과를 도 4에 제시하였다.In order to compare with the prior art, 1.5 ml of BACT/ALERT FA Plus (BioMerieux, France) was added to the culture medium of aerobic bacteria and 1.5 ml of BACT/ALERT FN Plus 17 (BioMerieux, France) was added to the culture medium of anaerobic bacteria. In place of the composition prepared in 1, each was injected, and after 13 hours, the color change was observed and photographed in units of 1 hour, and the results of photographing are presented in FIG.
도 3 및 도 4에 제시된 바와 같이, 본 발명에서 제공하는 미생물 검출용 조성물의 경우에는 실험실시 후 13시간이 지난 후부터 색변화가 확실히 관찰되는 반면에, 종래기술의 조성물을 이용한 실험에서는 13시간 후에는 색변화가 명확하지 않고, 17시간이 지난 후에야 명확한 색변화가 관찰되는 것을 알 수 있다.As shown in FIGS. 3 and 4, in the case of the composition for detecting microorganisms provided in the present invention, the color change was clearly observed after 13 hours after the test, whereas in the experiment using the composition of the prior art, after 13 hours It can be seen that the color change is not clear, and a clear color change is observed only after 17 hours.
상기의 실시예 등에서 나타난 바와 같이, 본 발명에서 제공하는 미생물 검출용 조성물을 이용할 경우에는 종래의 기술보다 더 빠른 시간에 미생물을 검출할 수 있는 것으로 나타나 탁월한 효과가 있다는 것을 알 수 있다.As shown in the above examples, it can be seen that when the composition for detecting microorganisms provided in the present invention is used, it is possible to detect microorganisms in a faster time than in the prior art, and thus has an excellent effect.
상기의 실시예 등은 본 발명에서 제공하는 조성물을 더욱 자세히 설명하기 위한 예시일 뿐, 본 발명의 범위를 한정하고자 하는 것이 아니다.The above examples and the like are merely examples for explaining the composition provided in the present invention in more detail, and are not intended to limit the scope of the present invention.
본 발명은 산업상이용가능하다.The present invention is industrially applicable.
Claims (4)
<화학식 1>
상기 화학식 1에서 R1 및 R2는 각각 독립적으로 H 또는 SO3H 중에서 선택되는 치환기이고, m 및 n은 각각 독립적으로 1 내지 3의 정수이다.
It relates to a composition for detecting microorganisms, wherein the composition is formed by including a compound represented by Formula 1 below, a universal indicator, and a silicone colorant in a solvent.
<Formula 1>
In Formula 1, R 1 and R 2 are each independently a substituent selected from H or SO 3 H, and m and n are each independently an integer of 1 to 3.
The method of claim 1, wherein the content of each component forming the composition is formed by including 150 to 200 parts by weight of the universal indicator and 10 to 20 parts by weight of the silicone colorant based on 100 parts by weight of the compound represented by Formula 1. A composition for detecting microorganisms
The composition for detecting microorganisms according to claim 1, wherein the solvent is selected from substituted or unsubstituted alcohol having 1 to 6 carbon atoms or dimethylformaldehyde.
The method according to any one of claims 1 to 3, wherein the composition for detecting microorganisms is formed by further adding 0.001 to 0.01 parts by weight of sodium hydroxide when the content of the compound represented by Formula 1 is set to 100 parts by weight. Composition for detecting microorganisms, characterized in that
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| KR20000071045A (en) * | 1997-12-12 | 2000-11-25 | 에프.지.엠. 헤르만스 | Sensor device for detecting microorganisms, and method therefor |
| EP1394219A1 (en) * | 1999-06-09 | 2004-03-03 | Carnegie-Mellon University | PH sensitive cyanine dyes as reactive fluorescent reagents |
| KR20100115301A (en) * | 2009-04-17 | 2010-10-27 | 주식회사 디케이씨코포레이션 | Novel cyanine compound for labeling biomolecule and preparation method thereof |
| KR20130017111A (en) * | 2011-08-10 | 2013-02-20 | 주식회사 디케이씨코포레이션 | A process of labeling biomolecules by using dye compounds with vinylsulfone group |
| KR20180110858A (en) * | 2017-03-30 | 2018-10-11 | (주)바이오액츠 | Dye compound and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000071045A (en) * | 1997-12-12 | 2000-11-25 | 에프.지.엠. 헤르만스 | Sensor device for detecting microorganisms, and method therefor |
| EP1394219A1 (en) * | 1999-06-09 | 2004-03-03 | Carnegie-Mellon University | PH sensitive cyanine dyes as reactive fluorescent reagents |
| KR20100115301A (en) * | 2009-04-17 | 2010-10-27 | 주식회사 디케이씨코포레이션 | Novel cyanine compound for labeling biomolecule and preparation method thereof |
| KR20130017111A (en) * | 2011-08-10 | 2013-02-20 | 주식회사 디케이씨코포레이션 | A process of labeling biomolecules by using dye compounds with vinylsulfone group |
| KR20180110858A (en) * | 2017-03-30 | 2018-10-11 | (주)바이오액츠 | Dye compound and preparation method thereof |
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