KR20230089696A - A long term storage method of Tenebrio molitor larvae - Google Patents

Abstract

The present invention relates to a long-term storage method for Tenebrio molitor larvae, which can maintain a resting phase state of the Tenebrio molitor larvae for a long period of time and provide larvae, pupae, or adults according to the purpose. According to the long-term storage method for the Tenebrio molitor larvae of the present invention, the Tenebrio molitor larvae can be stored for a long time with a high survival rate without loss of individuals during the resting phase, and the productivity of farms can be effectively increased by breaking the pause properly at the moment of need and providing a desired product in the form of larvae, pupae or adults.

Description

A long term storage method of Tenebrio molitor larvae}

The present invention relates to a long-term storage method of brown mealworm larvae capable of maintaining the dormant state of brown mealworm larvae for a long period of time and providing larvae, pupae or adults according to the purpose.

Recently, as the diversity and utilization of useful insects known as natural materials have been reported, consumer interest in them is also increasing. In Korea, insects that can be manufactured and distributed as food are registered and managed through support and policies for the insect industry.

Brown mealworm ( Tenebrio molitor ) is an insect belonging to the Coleoptera Tenebrionidae and is widely used for food in China, Cambodia, Thailand and Mexico, but is mostly used as pet food in Korea. However, interest in natural product-derived functional foods is currently growing, and demand for insects that are believed to contain various substances that can be developed as natural new drugs is gradually increasing. As it has been reported that they can be used in various ways such as antithrombosis and fatty liver, studies to use them for food and medicine are being actively conducted.

The larvae of the brown mealworm are also called mealworms and are already being made and sold as snacks and dishes on the market, and it is known that many countries in Europe are also mass-breeding mealworms for edible sales. In Korea, as a result of a study on the edible use and toxicity of brown mealworms in 2014 by the Rural Development Administration in Korea, it was found that they were harmless to the human body, and the Ministry of Food and Drug Safety also recognized brown mealworm larvae as food. The Ministry of Agriculture, Food and Rural Affairs of Korea suggested calling it 'Gosoae', which means 'savory caterpillar', to promote the eating of brown mealworm larvae (mealworms). It is in the limelight as a future food substitute for meat. In addition, it has a high protein and unsaturated fatty acid content, so it has an excellent effect on preventing cardiovascular diseases.

On the other hand, brown mealworms are particularly useful in the larvae state and can be used as food, but it is difficult for farms to adjust production according to the demand of larvae, and labor and costs are wasted, which is pointed out as a problem. Therefore, it is difficult to properly maintain the production and release of brown gourd larvae in farms, and research on appropriate methods to solve this is necessary.

While studying a method for long-term storage of brown mealworm larvae in dormancy, the combination of appropriate low temperature conditions and dormancy breaking temperature conditions maintains brown mealworm larvae with a high survival rate for a long period of time, and pupation occurs through control of dormancy breaking conditions. Alternatively, it was confirmed that larvae maintenance could be controlled and the present invention was confirmed.

In order to achieve the above object, the present invention is 1) breeding brown gourd 6 to 8 instar larvae at 10 to 16 ° C; and 2) dormant breaking of the brown gourd larvae raised in step 1) at 18 to 30 ° C.; It provides a long-term storage method of brown gourd larvae, including a.

In addition, the present invention is 1) breeding brown goose 6 to 8 instar larvae at 10 to 16 ° C; and 2) dormant breaking of the brown gourd larvae raised in step 1) at 18 to 30 ° C.; It provides a method for controlling the resting period of brown gourd larvae, including.

According to the long-term storage method for brown mealworm larvae of the present invention, brown mealworm larvae can be stored for a long period of time with a high survival rate without loss of objects in the dormant period, and can be appropriately broken at the necessary moment to provide the desired product in the form of larvae, pupae or adults. Therefore, it can effectively increase the productivity of farm households.

1 is a schematic diagram of a long-term storage method for brown gourd larvae according to the present invention.

The present invention relates to a method for long-term storage of brown mealworm larvae and a method for controlling the resting period of brown mealworm larvae.

According to the present invention, it is possible to provide larvae, pupae or adults at a desired time by maintaining the brown mealworm larvae in a dormant state for a long period of time. In particular, according to the method of the present invention, brown geese can be stored for a long time with high survival rate without object loss, and the low-temperature storage temperature and dormant breaking temperature can be appropriately selected according to the type of final target product to transform brown geese into larvae, pupae or adults. can be adjusted

Hereinafter, the present invention will be described in detail.

The present invention is 1) breeding 6 to 8 instar larvae of brown goose at 10 to 16 ° C; and 2) dormant breaking of the brown gourd larvae raised in step 1) at 18 to 30 ° C.; It relates to a long-term storage method of brown gourd larvae, including.

In the method of the present invention, wheat bran can be fed as a food source for the larvae while breeding the 6th to 8th instar larvae at 10 to 16 ° C. In the present invention, it was confirmed that when the brown gourd larvae are reared at a temperature of less than 10 ° C, very high mortality occurs regardless of the presence or absence of a food source, and when reared at a temperature of 25 ° C or higher, the larvae state is not maintained, and the average After 63 days, it was confirmed that all of them were pupated. These results can add low-temperature conditions for long-term storage of larvae of 6 to 8 instars of brown goose, but too low a temperature can rather cause the death of insects, and normal breeding temperatures do not properly maintain the larvae, , indicating that very detailed temperature control is required for long-term storage of brown mealworm larvae.

Therefore, the optimal temperature condition for maintaining the brown gourd larvae in a dormant state is 10 to 16 ° C, and it is most preferable to breed while feeding wheat bran at this temperature. In the present invention, the resting period means a period in which brown gourd larvae show a direct response to an inappropriate environment and a decrease in metabolic rate. When the environment improves again, growth can begin again, and inducing this is called break-in.

The dormant state of brown gourd larvae means that growth does not proceed immediately in unfavorable environments, but development proceeds immediately when an environment suitable for growth is reached. is distinguished from That is, in the present invention, it is possible to quickly control the growth stop and progress of brown gourds through optimal temperature control.

By providing the optimal temperature conditions as described above, larvae of 6 to 8 instars can maintain a larval state for 100 to 160 days, which is longer than the average larva maintenance period of 63 days during general larva breeding, and preferably 130 days to 150 days, most preferably 135 days to 145 days. That is, according to the present invention, since the state of brown gourd larvae can be maintained up to 160 days, preferably up to 145 days, farmhouses can select the time to induce pupation by breaking dormancy within the above period.

On the other hand, even if the dormant state of brown mealworm larvae is maintained for a long time, commercial value is damaged if dormancy is not properly broken, so it is important to ensure that the individual does not die and that the individual's growth can be properly maintained even after dormancy breaking. do. In addition, since brown mealworms have commercial value mainly as larvae, it is necessary to adjust appropriately according to the customer's order, depending on whether the product is provided by maintaining the larva state in the farmhouse or by inducing it to a pupa or adult state.

Accordingly, in the present invention, it may be characterized in that brown gourd larvae stored for a long time at 10 to 16 ° C are dormant at 18 to 30 ° C.

In the case of long-term storage at a low temperature of 10 to 12 ° C., even if dormant breaking was attempted at a temperature of 18 to 30 ° C., pupae were not or hardly induced after 2 to 3 weeks, and even after dormant breaking, the larvae state maintained for a long time.

Therefore, in the present invention, when the state to be finally provided is larvae, the larvae of step 1) are reared at 10 to 12 ° C, and step 2) is performed at 18 to 30 ° C. More specifically, the larvae of step 1) may be reared at 10 to 12 ° C, and step 2) may be performed at 25 to 30 ° C.

Using the method of the present invention, in farms, if larvae that need to be produced after 140 days, for example, are stored for a long time at a low temperature at 10 ° C to 12 ° C, and then dormant breaking at 25 ° C to 30 ° C, it takes 3 weeks to start dormant breaking according to each storage condition. Alternatively, it can produce larvae of suitable weight for production within two weeks.

On the other hand, if the state to be finally provided is a pupa or an adult, the larvae of step 1) may be reared at 14 ° C to 16 ° C, and step 2) may be performed at 18 to 30 ° C. As a result, the larvae of step 1) may be reared at 14 ° C to 16 ° C, and step 2) may be performed at 30 ° C.

Using the method of the present invention, in farms, if imagos are needed after 140 days, for example, long-term storage at low temperature at 14 ° C to 16 ° C, then dormancy breaking at 18 ° C to 30 ° C, most preferably 30 ° C, only 3 weeks from the start of dormant breaking Since more than 90% can be induced into pupae and about 60% become adults, pupae or adults can be provided appropriately.

In particular, in the case of long-term storage of brown mealworm larvae at the low-temperature storage and dormancy breaking temperatures of the present invention, high larvae weight was achieved, and the larvae weight was high in the order of 15 ° C > 12 ° C > 10 ° C, effectively increasing farm productivity. can be improved

Although not limited thereto, the weight of the larvae after 1 week of dormant break of the present invention may be 0.06 to 0.15 g, and according to the method of the present invention, 0.1 g or more suitable for production after 2 to 3 weeks of do not break. Since larvae can be produced, it is possible to rapidly induce development of larvae that have been maintained in a dormant state while maintaining long-term survival at the time when brown gourd larvae are needed.

In another aspect, the present invention provides 1) breeding brown gourd 6 to 8 instar larvae at 10 to 16 ° C; and 2) dormant breaking of the brown gourd larvae raised in step 1) at 18 to 30 ° C.; It provides a method for controlling the resting period of brown gourd larvae, including.

In the present invention, step 1) may be a method characterized in that it is performed while feeding wheat bran as a food source, and step 1) may be characterized in that the dormant period is maintained for 100 to 160 days. That is, according to the present invention, the brown mealworm larval period and dormant period can be maintained as desired for up to 160 days, and the larval period of the brown mealworm can be controlled by appropriately selecting the start time of the dormancy breaking temperature. In addition, as described above, the combination of the cold storage temperature conditions and the resting temperature of the brown mealworm larvae affects the maintenance of the larva after induction of rest or the change to the pupal and adult stages, so through these adjustments, the final target brown meal Growth stages can be controlled.

Therefore, in the present invention, the control of the larval period includes control of the dormant period of the brown gourd larvae, maintenance of the larval state, or control of pupation.

The method for controlling the resting period of brown mealworm larvae of the present invention can be equally applied to the description of the long-term storage method for brown mealworm larvae. In this specification, in order to avoid complexity of description, description of overlapping contents is omitted.

The present invention will be described in detail through examples below. These examples are for illustrative purposes only and do not limit the scope of the present invention.

Example 1. Confirmation of long-term storage conditions for brown geese according to temperature and presence of food source

The brown geese being bred at the National Institute of Agricultural Sciences were individually bred by supplying 0.1 g of wheat bran to a Petri dish (60x15mm), and the experiment was conducted with the same humidity (40%) except for the temperature conditions. In order to stop the growth of brown geese and confirm the optimal conditions for long-term storage in the dormant period, 0.02 to 0.04 g of 6 to 8-week-old brown geese were bred under various low-temperature conditions. Specifically, 25 ℃, which is known as a normal breeding temperature, was used as a control group, and breeding was performed at low temperature conditions of 5 ℃, 10 ℃, 12 ℃, and 15 ℃, and all experimental groups were bred by 30 animals. In addition, wheat bran was fed as a food source under each temperature condition, and the effect of feeding as a food source was confirmed. Table 1 shows the survival rate of brown geese in each condition.

5℃ 10℃ 12℃ 15℃ 25℃ bran oil 60% 100% 97% 100% 96.6 bran radish 47% 66.6 76.6 100% 0%

As shown in Table 1, survival is well maintained at 25 ° C, which is a general optimum breeding temperature, but all become pupae after an average of 63 days, and long-term storage in the desired resting period could not be achieved. In the experimental group maintained and supplied with wheat bran, it was confirmed that it could be stored for a long period of time as a dormant period by maintaining a larval state without becoming a pupa while showing a high survival rate. In particular, under the above conditions, brown gourd larvae could be stored for a long time for about 140 days. On the other hand, in the experimental group raised at 5 ° C among low temperature conditions, the survival rate was less than 50% when wheat bran was not supplied, and the survival rate was 60% even when wheat bran was supplied, confirming that it was an unsuitable condition for long-term storage.

Through the above results, it was confirmed that the optimal conditions for long-term storage of 6 to 8 instar brown mealworm larvae in a dormant state were low temperature conditions of 10 to 15 ° C and wheat bran feeding conditions.

Example 2. Confirmation of break breaking conditions

Even if long-term survival was achieved as in Example 1, the individual could die during dormancy breaking, so an experiment was performed to confirm the optimal dormancy breaking conditions. In Example 1, dormancy breaking was performed on individuals who survived for 140 days by feeding and storing bran at 10 ° C to 15 ° C, and 10 larvae were randomly selected from each experimental group. The temperature for rest breakup was varied under conditions of 18 ° C, 25 ° C, and 30 ° C, and the weight was measured every week. The dormant breaking temperature conditions were given to the bran-fed breeding experimental group of Example 1, and the time points at which the pupae of the individuals first appeared in each condition are shown in Table 2 below.

[Table 2]

As shown in Table 2, all groups fed bran and stored at a low temperature at 15 ° C. pupae were confirmed in 3 weeks, 2 weeks, and 2 weeks, respectively, at dormant temperatures of 18, 25, and 30 ° C. In particular, under the condition of 30 ° C., about 60% of the individuals pupated at 2 weeks, and 90% at 3 weeks showed the best pupal production ability, and about 60% of them became adults under the above conditions. On the other hand, in the experimental group fed wheat bran and stored at a low temperature at 12℃, pupae were confirmed at 3 weeks only at 25℃ dormant break conditions. It was confirmed that the condition of the larvae was maintained for a long period of time. On the other hand, when dormancy is attempted at 18 ° C regardless of the cold storage temperature of 10 to 15 ° C, the time to confirm pupae is late compared to other temperature conditions, so it is suitable for long-term storage of larvae, but it is confirmed that the development is somewhat slower than other experimental groups. did

On the other hand, in the experimental group that was not fed wheat bran and stored at low temperature, all surviving individuals either died or did not grow normally even though they were fed wheat bran again and reared under different temperature conditions in this breakout experiment, so when stored for a long time in low temperature conditions, the supply of bran was reduced. confirmed to be essential.

The weight of each larva was compared for each condition at 1 week in the dormant breaking condition, and the results were compared through a non-parametric test (Mann-Whitney test). The weight change of the larvae in each condition is shown in Table 3 below.

[Table 3]

As can be seen in Table 3, it was confirmed that the weight of the larvae of the first week in the dormant breaking condition was high in the order of 15 ° C > 12 ° C > 10 ° C. When resting at 25 or 30 ° C after 10 to 12 ° C low temperature storage conditions, development proceeds with a weight of 0.1 g or more in all experimental groups, so larvae suitable for production can be obtained within 3 weeks or 2 weeks depending on each storage condition confirmed.

Through the above results, it was confirmed that the dormant larvae state can be maintained for a long period of time for 140 days by adding appropriate low-temperature conditions to brown goose, whose larvae are mainly used for food or breeding, and through the addition of appropriate dormant breaking temperature conditions It was confirmed that the pupalization time can be controlled. For example, in farms, brown gourd larvae can be stored for a long time in a dormant state for more than 140 days at 10 to 15 ° C, which is the low temperature storage temperature of the present invention. Larvae are provided by breeding, and if adults are requested, adults can be provided by breeding at 30 ° C after low temperature storage at 15 ° C.

Claims (12)

1) Breeding 6 to 8 instar larvae at 10 to 16 ° C; and
2) dormant breaking of the brown gourd larvae raised in step 1) at 18 to 30 ° C; A long-term storage method of brown gourd larvae comprising a.
The method according to claim 1, wherein step 1) is performed while feeding wheat bran as a food source.
The method of claim 1, wherein step 1) maintains the resting period for 100 to 160 days.
According to claim 1,
If the final state to be provided is a larva,
Raising the larvae in step 1) at 10 to 12 ° C,
The method of performing step 2) at 18 to 30 ° C.
According to claim 4,
Raising the larvae in step 1) at 10 to 12 ° C,
The method of performing step 2) at 25 to 30 ° C.
According to claim 1,
If the final state to be provided is a pupa or an adult,
Raising the larvae in step 1) at 14 ° C to 16 ° C,
The method of performing step 2) at 18 to 30 ° C.
According to claim 6,
If the final state to be provided is a pupa or an adult,
Raising the larvae in step 1) at 14 ° C to 16 ° C,
Where step 2) is performed at 30° C.
The method according to claim 1, wherein the weight of the larvae after 1 week of dormancy in step 2) is 0.06 to 0.15 g.
1) Breeding 6 to 8 instar larvae at 10 to 16 ° C; and
2) dormant breaking of the brown gourd larvae raised in step 1) at 18 to 30 ° C; A method for controlling the resting period of brown gourd larvae, comprising a.
The method of claim 9, wherein step 1) is performed while feeding wheat bran as a food source.
The method of claim 9, wherein step 1) maintains the resting period for 100 to 160 days.
10. The method of claim 9, wherein the control of the rest period includes control of the dormancy period of brown gourd larvae, maintenance of the larval state, or control of pupation.

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