KR20230087049A - A composition for improving hair or scalp condition comprising a plant extract as an active ingredient - Google Patents

Abstract

The present invention relates to a composition for improving the condition of hair or scalp comprising a plant extract as an active ingredient. According to one aspect, the composition comprising a plant extract as an active ingredient inhibits the activity of hair loss mediating factors, promotes growth of hair papilla cells, reduces the expression of androgen receptor (AR) and cell destruction-related factors, and increases the expression of hair follicle cell activation and regeneration-related factors, to be effectively used to improve the condition of hair or scalp.

Description

A composition for improving hair or scalp condition comprising a plant extract as an active ingredient {A composition for improving hair or scalp condition comprising a plant extract as an active ingredient}

It relates to a composition for improving hair or scalp condition comprising a plant extract as an active ingredient.

Hair is an appendage of the skin and is divided into a hair shaft corresponding to the outer part of the skin and a hair root part surrounded by hair follicles inside the scalp. The hair of the hair shaft is composed of three parts: cuticle, cortex, and medulla from the outermost part. The cuticle is a stratum corneum formed to protect the inside of the hair from external stimuli and determines the wettability and gloss of the hair. The cortex and medulla are responsible for maintaining the shape of the hair and are filled with fibers made of a protein called keratin. Hair is formed in hair follicles at the hair root, and there are about 100,000 to 150,000 hair follicles on the scalp. The hair follicle is in the form of a three-layer cylindrical shape surrounding the hair in the center, and consists of the dermal sheath, the outer root sheath, and the inner root sheath from the outermost. The root part of the hair follicle is called the hair bulb, and hair is generated and grown in that part. It is a bulb-shaped convex shape at the bottom of the hair root, and contains a structure called the dermal papilla, which has a papillary protrusion. The dermal papilla is a central part that controls hair growth. It comes out of the dermal cell layer and has many capillaries and autonomic nerves, supplying various nutrients, protein synthetase, hormones, and oxygen necessary for hair growth. The dermal papilla is also the site where male hormones act in male pattern baldness. The part where cell division occurs according to the control in the dermal papilla is called the matrix, and cell division and proliferation are constantly repeated. There are sebaceous glands in the diagonal direction of the dermal papilla, which provide luster to the hair and supply oil.

The cycle of hair refers to the cycle of creation and extinction of each hair. The mother cycle repeats a certain cycle of development, growth, hair loss, and development. Usually, the corresponding cycle is classified into anagen, catagen, and telogen. The anagen phase is the time when hair cells divide and new hair bulbs are created, and body hair begins to grow in the hair follicle. It is a period in which hair grows due to active hair follicle activity and cell division. It lasts about 2 to 6 years and accounts for 85% of all hair. The catagen phase is the time when hair cells stop dividing and start preparing for hair loss. The hair root goes up, the dermal papilla is detached from the hair bulb, and the hair bulb contracts the tissue and changes to become thin and long like a club. It takes about 2-3 weeks and accounts for 5% of all hair. It is the period when the dermal papilla and hair fall off and the dermal papilla turns round, and the cell activity of the hair follicle completely disappears. The hair falls out due to being pushed by growing hair or by mechanical action such as combing. It accounts for 10% of all hair.

Hair loss progresses as androgens or active oxygen stimulate dermal papilla cells. It occurs when dermal papilla cells secrete substances that destroy hair follicles or inhibit the secretion of growth factors that grow hair. When excessive secretion of sebum occurs in the sebaceous glands, inflammation is induced in the dermal papilla, and nutrient supply becomes inevitable, resulting in hair loss. Stress is also one of the factors that cause hair loss. Corticotropin-releasing factor, a hormone involved in stress response, changes the expression of cytokines in dermal papilla cells, induces early degeneration of hair follicles, and suppresses hair growth to cause hair loss.

A major factor in male pattern baldness is the effect of abnormal androgens. Androgens are the collective term for hormones that affect the growth and development of the male reproductive system. Testosterone, one of the androgens, is converted into dihydrotestosterone, an active male hormone, by an enzyme called 5α-reductase. When the activated dihydrotestosterone binds to the androgen receptor (AR) of the dermal papilla in the frontal and parietal regions, a cell destruction signal is transmitted to the DNA of the hair cells. When DKK-1 (Dickkopf-1) and TGF-β1 (Tumor Growth Factor β-1), which are cell apoptosis factors, are produced by this signal, hair cells are destroyed and converted to a catagen phase, resulting in hair loss. Relatively, women have only half of 5-alpha-reductase compared to men, so the frequency of hair loss by the same mechanism is considerably lower than that of men.

In general, hair growth treatment agents that prevent the occurrence of DHT for the treatment of male pattern baldness are common, and Finasteride is a representative example. However, if women take it for a long time, there is a problem in that the probability of giving birth to a deformed child is high and in the case of men, side effects such as erectile dysfunction occur. Recently, studies on methods of using the proliferation of hair follicle cells without side effects have been actively conducted. VEGF (Vascular Endothelial Grwoth Factor) and BMP 2 (Bone Morphogenetic Protein 2) play very important roles, and especially Wnt/β-catenin is in a very important position in hair follicle cell activation and regeneration.

Philadephus schrenckii is a deciduous shrub native to the valleys of the Korean Peninsula. The leaves are specified as edible parts in the food raw materials notified by the Ministry of Food and Drug Safety. Gogwang tree has been known since ancient times to have the effect of clearing heat and detoxifying and relieving spleen.

Securinega suffruticosa is a deciduous broad-leaved shrub native to the foothills of the Korean Peninsula or along sunny rivers. The leaves and fruits are specified as edible parts in the food raw materials notified by the Ministry of Food and Drug Safety. It has been known for a long time that clown clownfish has the effects of active blood seogeun (活血舒筋), strong muscle bone (强筋骨), and tendon hypertrophy (健脾益胃).

Baby Suyeong ( Rumex acetosella ) is a perennial plant that grows wild in the fields or roadsides of the middle and south of Hanbandu. The leaves and shoots are specified as edible parts in the food raw materials notified by the Ministry of Food and Drug Safety. Since ancient times, Aegisuyeong has been known to have the efficacy of clearing heat, clearing heat, diuretic, warming heat, and killing insects.

However, the use of the plant extract for the prevention, improvement, or treatment of hair and scalp conditions is not known, and no mechanistic studies have been conducted. Therefore, the present inventors conducted direct efficacy studies on the prevention, improvement, and treatment of hair and scalp health with plant extracts.

Korean Patent Publication No. 10-2016-0054087

One aspect is a health functional food composition for improving hair or scalp condition containing at least one selected from the group consisting of Philadephus schrenckii extract, Securinega suffruticosa extract and Rumex acetosella extract as an active ingredient is to provide

To provide a cosmetic composition for improving hair or scalp condition comprising at least one selected from the group consisting of other Philadephus schrenckii extract, Securinega suffruticosa extract and Rumex acetosella extract as an active ingredient.

Another aspect is to provide a pharmaceutical composition for preventing or treating hair loss comprising at least one selected from the group consisting of Philadephus schrenckii extract, Securinega suffruticosa extract and Rumex acetosella extract as an active ingredient is to do

Another aspect is a skin external preparation for improving hair or scalp condition containing at least one selected from the group consisting of Philadephus schrenckii extract, Securinega suffruticosa extract and Rumex acetosella extract as an active ingredient Provides is to do

One aspect is a health functional food composition for improving hair or scalp condition containing at least one selected from the group consisting of Philadephus schrenckii extract, Securinega suffruticosa extract and Rumex acetosella extract as an active ingredient to provide.

The extract may be an extract extracted by a solvent from the whole aerial part of the tree, a part thereof, or a material derived therefrom, respectively. The part may be a tree stem, root, leaf, flower, petal, seed (seed), fruit flesh, fruit skin, or fruit, and preferably may be a leaf. Whole trees, parts thereof, or materials derived therefrom used for extraction may be ground or minced or suitably dried.

The extract may be extracted by any extraction method such as hot water extraction, solvent extraction, distillation extraction, supercritical extraction, etc., which has been conventionally used to extract natural plants, and is preferably extracted with water, an organic solvent, or a mixture thereof. characterized by being The organic solvent may be at least one selected from the group consisting of alcohols having 1 to 4 carbon atoms, such as ethanol, methanol, isopropanol, and butanol. In addition, the alcohol may contain alcohol. The alcohol concentration of the aqueous alcohol solution is 1 to 99.5 (v / v)%, for example, 10 to 99.5 (v / v)%, 1 to 70 (v / v)%, 1 to 40 (v / v)% , 5 to 50 (v/v)%, 5 to 40 (v/v)%, 10 to 50 (v/v)%, or 10 to 40 (v/v)%.

The extraction is 3 to 50 (w / w) times the extraction solvent with respect to the plant, for example, 3 to 40 (w / w) times, 3 to 30 (w / w) times, 5 to 50 (w / w) times w) times, 5 to 40 (w/w) times, 5 to 30 (w/w) times, or 4 to 40 (w/w) times. For example, it may include adding 3 to 50 kg of the extraction solvent with respect to 1 kg of materials derived from Kogwangnamu, Gwangsanji, and Aegisuyeong.

The extraction may be performed by hot water extraction, immersion extraction, supercritical extraction, subcritical extraction, reflux cooling extraction, steam distillation, high pressure enzymatic digestion, ultrasonic extraction, elution, compression, and the like.

The extraction is performed at 4 °C to 90 °C, for example, 4 °C to 80 °C, 4 °C to 70 °C, 5 °C to 80 °C, 10 °C to 70 °C, 15 °C to 70 °C or 20 °C to 70 °C. it may be The extraction time may vary depending on the selected temperature and may be 1 hour to 2 months, for example, 1 hour to 1 month, 1 hour to 10 days, 1 hour to 5 days, 1 hour to 3 days, 1 hour to 2 days. , 1 hour to 1 day, 1 hour to 10 hours, 2 hours to 1 month, 2 hours to 15 days, 2 hours to 10 days, 2 hours to 5 days, 2 hours to 3 days, 2 hours to 2 days, 2 hours to 1 day or 2 hours to 10 hours. The extraction may include mixing the whole, part of, or materials derived therefrom from the whole of Kogwangnamu, Gwangsanji and Aegisuyeong in the solvent and leaving it for a certain period of time. The standing may include suitable agitation. The extraction may be repeated one or more times, for example 1 to 5 times.

In the extraction, plant residues and extracts may be separated by a known method such as filtration. The extraction may also include removing the solvent from the obtained extract by a known method such as concentration under reduced pressure. The extraction may also include preparing a dried extract by drying the obtained extract such as lyophilization and spray drying.

In one embodiment, each of the Kogwang tree extract, the clownfish extract, and the baby sulphur extract is 0.0001% to 90.0% by weight, for example, 0.01% to 60% by weight, 0.01% to 40% by weight based on the total weight of the composition. %, 0.01% to 30%, 0.01% to 20%, 0.01% to 10%, 0.01% to 5%, 0.05% to 60%, 0.05% to 40%, 0.05% to 30% by weight, 0.05% to 20% by weight, 0.05% to 10% by weight, 0.05% to 5% by weight, 0.1% to 60% by weight, 0.1% to 40% by weight, 0.1% by weight % to 30% by weight, 0.1% to 20% by weight, 0.1% to 10% by weight, 0.1% to 5% by weight, 5 to 20% by weight, 5 to 18% by weight, 6 to 15% by weight, 8 to It may be included in 15% by weight or 7 to 10% by weight. However, in the case of long-term intake for the purpose of health and hygiene or health control, it may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

The Kogwang tree extract, clown hair extract, or Arabidopsis extract may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method.

In one embodiment, the hair or scalp condition improvement is to prevent or improve hair damage; promote hair growth; Or it may be hair loss suppression, prevention or improvement.

In one embodiment, the extract may inhibit 5α-reductase, a hair loss mediating factor, and promote the proliferation of dermal papilla cells. In addition, it reduces the expression of one or more selected from the group consisting of androgen receptor (AR) and cell destruction-related factors, Dickkopf-1 (DKK-1) and Transforming Growth Factor Beta 1 (TGF-β1), and activates hair follicle cells and regeneration-related factors, Insulin like growth factor-I (IGF-1), Lymphoid Enhancer Binding Factor 1 (LEF1), and Bone morphogenetic protein 2 (BMP2). The present inventors found this efficacy of the extract and confirmed that the extract has an effect of improving hair or scalp conditions.

The "health functional food" refers to a food manufactured and processed by extracting, concentrating, refining, mixing, etc., a specific ingredient as a raw material or a specific ingredient contained in a food raw material for the purpose of supplementing health. It refers to food designed and processed to sufficiently exert biological control functions such as defense, regulation of circadian rhythm, prevention and recovery of disease, etc. The health functional food composition may perform functions related to hair or scalp condition improvement.

There is no particular limitation on the type of food. Examples of foods to which the above substances may be added include powders, granules, tablets, capsules, pills, gels, jellies, suspensions, emulsions, syrups, tea bags, leached teas, and formulations selected from the group consisting of health drinks, etc. It includes all health foods in the usual sense.

The health beverage composition of the present invention may include various flavoring agents or natural carbohydrates as additional components, like conventional beverages. The aforementioned natural carbohydrates may include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, natural sweeteners such as dextrin and cyclodextrin, and synthetic sweeteners such as saccharin and aspartame. The proportion of the natural carbohydrate is generally about 0.01 to 10 g, preferably about 0.01 to 0.1 g per 100 ml of the composition of the present invention.

The health functional food may include food additives acceptable in food science, and may include appropriate carriers commonly used in the manufacture of health functional food.

In addition to the above, the composition of the present invention contains various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages; and the like. In addition, the composition of the present invention may include fruit flesh for preparing natural fruit juice, fruit juice beverages, and vegetable beverages. These components may be used independently or in combination. The ratio of these additives is not critical, but is generally selected in the range of 0.01 to 0.1 part by weight per 100 parts by weight of the composition of the present invention.

Another aspect provides a cosmetic composition for improving hair or scalp condition comprising at least one selected from the group consisting of an extract of Kogwang tree, an extract of Buddensis chinensis, and an extract of Arabidopsis, as an active ingredient.

In one embodiment, the hair or scalp condition improvement is to prevent or improve hair damage; promote hair growth; Or it may be hair loss suppression, prevention or improvement.

At this time, the extract and its effects are as described above.

The cosmetic composition may be prepared in various types of formulations, such as solutions, suspensions, emulsions, pastes, gels, creams, lotions, shampoos, surfactant-containing cleansing, tonics, and scaling, for example. For example, hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, hair wax, hair aerosol. , hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, hair waving agent, hair bleach, hair gel, hair glaze, hair dressing, hair lacquer, hair moisturizer, It can be prepared in the form of a hair mousse or hair spray. Compositions of such formulations may be prepared according to conventional methods in the art. The blending amount of the additional ingredients such as the moisturizing agent can be easily selected by those skilled in the art within a range that does not impair the objects and effects of the present invention.

The cosmetic composition may further include functional additives and ingredients included in general cosmetic compositions in addition to the active ingredients disclosed herein, and commonly used purified water, thickeners, preservatives, stabilizers, solubilizers, surfactants, carriers, A flavoring agent or a combination thereof may be further included. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extracts. Alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, humectants, viscosity modifiers, emulsions, stabilizers, UV scattering agents, UV absorbers, coloring agents, perfumes, and the like may be exemplified as the carrier. Compounds/compositions that can be used as the alcohol, oil, surfactant, fatty acid, silicone oil, wetting agent, humectant, viscosity modifier, emulsion, stabilizer, UV scattering agent, UV absorber, coloring agent, fragrance, etc. are already known in the art. Since there is, those skilled in the art can select and use an appropriate corresponding material / composition. In addition, the cosmetic composition, if necessary, sunscreen, antioxidants (butylhydroxyanisole, gallic acid propyl, ellisorbic acid, tocopheryl acetate, butylated hydroxytoluene, etc.), preservatives (methylparaben, butylparaben , propylparaben, phenoxyethanol, imidazolidinylurea, chlorphenesin, etc.), colorant, pH adjuster (triethanolamine, citric acid, citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid , sodium succinate, sodium hydroxide, sodium hydrogen phosphate, etc.), humectants (glycerin, sorbitol, propylene glycol, butylene glycol, hexylene glycol, diglycerin, betaine, glycereth-26, methylglue Seth-20, etc.) and lubricants may be further added.

In addition, in the cosmetic composition of each formulation, appropriate components may be selected and blended according to the cosmetic formulation or purpose of use. Since the formulation components and methods may follow conventional techniques, detailed descriptions thereof are omitted herein.

In addition, the cosmetic composition may be used by a method such as directly applying or dispersing to the hair or scalp. The hair to which the composition of the present invention is applied includes hair roots and hair follicles of the head, hair, eyelashes, eyelashes, beard, armpits, pubic hair, and all parts of the body with hair roots and hair follicles.

Another aspect provides a pharmaceutical composition for preventing or treating hair loss comprising, as an active ingredient, at least one selected from the group consisting of an extract from the genus chinensis, an extract from the genus chinensis, and an extract from A.

At this time, the extract and its effects are as described above.

The term "pharmaceutical composition" can refer to a molecule or compound that imparts some beneficial effect upon administration to a subject. Beneficial effects may include enabling diagnostic determination; amelioration of a disease, symptom, disorder or condition; reducing or preventing the occurrence of a disease, condition, disorder or condition; and responding to a disease, symptom, disorder or condition in general. In the present invention, the pharmaceutical composition may give a beneficial effect on hair loss.

The term "prevention" refers to partially or completely delaying or preventing the onset or recurrence of a disease, disorder, or its attendant symptoms, preventing the acquisition or re-acquisition of a disease or disorder, or the risk of acquiring a disease or disorder. tells how to reduce For example, the prevention refers to any action that inhibits or delays the occurrence of hair loss symptoms by administering the composition according to the present invention.

The term “treatment” includes inhibition, alleviation or elimination of the development of a disease, such as hair loss.

The term "improvement" may refer to any activity that at least reduces a parameter related to alleviation or treatment of a condition, for example, the degree of hair loss symptoms.

The pharmaceutical composition can be administered parenterally during clinical administration and can be used in the form of a general pharmaceutical preparation. Parenteral administration may refer to administration through an administration route other than oral, such as rectal, intravenous, peritoneal, intramuscular, arterial, transdermal, nasal, inhalation, ocular and subcutaneous administration. When the pharmaceutical composition of the present invention is used as a medicine, it may additionally contain one or more active ingredients exhibiting the same or similar functions.

Types of pharmaceutically active ingredients capable of delivering the active ingredient into the subject include anticancer agents, contrast agents (dye), hormones, anti-hormonal agents, vitamins, calcium agents, mineral preparations, saccharide preparations, organic acid preparations, protein amino acid preparations, detoxifying agents, and enzymes. Preparations, metabolic preparations, diabetes concomitant medicines, tissue regeneration medicines, chlorophyll preparations, pigment preparations, tumor medicines, tumor treatment agents, radiopharmaceuticals, tissue cell diagnostic agents, tissue cell therapy agents, antibiotics preparations, antiviral agents, complex antibiotics preparations, chemicals Therapeutic agents, vaccines, toxins, toxoids, antitoxins, leptospira serum, blood products, biological agents, analgesics, immunogenic molecules, antihistamines, allergy medications, non-specific immunogenic agents, anesthetics, stimulants, psychoactive agents, small molecule compounds, nucleic acids, It may include aptamers, antisense nucleic acids, oligonucleotides, peptides, siRNAs, and micro RNAs.

The pharmaceutical composition may be prepared by further including one or more pharmaceutically acceptable carriers. A pharmaceutically acceptable carrier may be a mixture of saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, and one or more of these components, and, if necessary, antioxidants and buffers. , bacteriostatic agents and other conventional additives may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to prepare formulations for injections such as aqueous solutions, suspensions, and emulsions, pills, capsules, granules, or tablets. Furthermore, it can be preferably formulated according to each disease or component by an appropriate method in the art.

When formulating the pharmaceutical composition, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for the suppository, Witepsol, Macrogol, Tween 61, cacao butter, liurine fat, glycerogeratin and the like may be used.

The pharmaceutical composition may include carbohydrates such as glucose, sucrose or dextran, antioxidants such as ascorbic acid or glutathione, chelating agents, and small molecules in order to increase stability or absorption. Proteins or other Stabilizers can be used as pharmaceuticals.

Another aspect provides a method of treating hair loss comprising administering the pharmaceutical composition to a subject.

The term “administering” means providing a pharmaceutical composition to a subject by any suitable method.

A pharmaceutically effective amount and an effective dosage of the pharmaceutical composition may vary depending on the formulation method, administration method, administration time and/or route of administration of the pharmaceutical composition. In addition, the type and degree of response to be achieved by administration of the pharmaceutical composition, the type of subject to be administered, age, weight, general health condition, symptom or degree of disease, gender, diet, excretion, simultaneous or It may vary according to various factors including drugs and other components of the composition used together in this case, and similar factors well known in the medical field. A person of ordinary skill in the art can readily determine and prescribe an effective dosage for the desired treatment. Administration of the pharmaceutical composition according to the present invention can be administered once a day, divided into several administrations. Therefore, the dosage is not intended to limit the scope of the present invention in any way. The dosage of the pharmaceutical composition may be 1 ug/kg/day to 1,000 mg/kg/day.

The subject may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat, or cat. The subject may be an individual in need of hair loss treatment.

Another aspect provides an external preparation for skin for improving hair or scalp condition, which contains at least one selected from the group consisting of an extract of Kogwang tree, an extract of H. chinensis, and an extract of Arabidopsis, as an active ingredient.

At this time, the extract and its effects are as described above.

The external skin preparation may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof. The external skin preparation is a component usually used in external preparations for skin such as cosmetics or pharmaceuticals, for example, water-based components, oil-based components, powder components, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, and fragrances. , colorants, various skin nutrients, or combinations thereof and may be suitably blended as needed. The external skin preparations include metal sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, bellapamil, licorice extract, glabridin, and calin. Hot-water extracts of fruits, various herbal medicines, tocopherol acetate, glycyrrhizic acid, tranexamic acid and its derivatives or salts and other drugs, vitamin C, magnesium ascorbate phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, Sugars, such as trehalose, etc. can also be mix|blended suitably.

The skin includes all skin areas of the body, including the face, hands, arms, legs, feet, chest, stomach, back, buttocks, and scalp.

The terms, methods, and effects described for the above invention are equally applied between each invention.

According to the composition containing a plant extract according to one aspect as an active ingredient, inhibits the activity of hair loss mediating factors, promotes the growth of dermal papilla cells, reduces the expression of androgen receptor (AR) and cell destruction related factors, and reduces hair follicles It has the effect of increasing the expression of factors related to cell activation and regeneration, so it can be effectively used to improve hair or scalp conditions.

Figure 1 is a graph comparing the 5α-reductase enzyme inhibitory activity levels when treated with the H. clownfish extract.
Figure 2 is a graph confirming the effect on cytotoxicity and cell proliferation in dermal papilla cells when treated with gogwangnamu, clownfish, or baby swimming extract.
Figure 3 is a graph confirming the expression inhibition of the androgen receptor (AR) when treated with a gogwangnamu, clown clownfish, or baby suyeong extract.
Figure 4 is a graph confirming the expression inhibition of DKK-1 when treated with clown clownfish or A.
Figure 5 is a graph confirming the inhibition of expression of TGF-β1 when treated with gogwangnamu, clownfish or baby suyeong extract.
Figure 6 is a graph confirming the increase in the expression of IGF-1 when treated with clownfish or A.
Figure 7 is a graph confirming the increase in expression of LEF1 when treated with extracts of Kogwangnamu, Gwangsansari, or Aegis suyeong.
Figure 8 is a graph confirming the increase in the expression of BMP 2 when treated with extracts of Gogwang tree or Arabidopsis.

It will be described in more detail through the following examples. However, these examples are intended to illustrate one or more specific examples, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of plant extract

The plant extract contained in the composition of the present invention was prepared by the following process.

First of all, domestically grown wild trees, clownfish, or baby suyeong were purchased, washed with distilled water, and then completely dried in a cool place without sunlight until there was no change in weight. Then, 30% fermented alcohol (30 times, w/w) was added to each completely dried dry matter. At this time, the mixing ratio of the dry matter and 30% fermented alcohol was 30 times the weight ratio. Next, 30% fermented alcohol mixed with the dried product was put in a constant temperature water bath and stirred and extracted at 60 ° C. for 5 hours. The extracted sample was filtered through a housing filter (10 um), concentrated under reduced pressure, and dried to obtain each powder.

Experimental Example 1: Confirmation of 5α-reductase enzyme activity inhibitory effect

The inhibitory ability of the 5α-reductase enzyme of the H. clownfish extract obtained in Example 1 was confirmed.

5α-reductase reduces testosterone to produce dihydrotestosterone (DHT). After reacting rat liver homogenate with testosterone, the ability to inhibit 5α-reductase enzyme activity was measured using a method for quantifying testosterone.

First, 7-week-old male SD rats were prepared and anesthetized using ethyl ether. After laparotomy of SD rats, the hepatic portal vein was nicked, and cold STM buffer (270 mM Sucrose, 10 mM Tris-HCl, pH7.5, 1 mM MgCl2, G-biosciences, USA) was injected into the left ventricle using a syringe. The STM buffer was perfused into the liver until the color of the liver changed from dark red to light pink. Then, the liver was removed, transferred to a beaker, cut with scissors, and then homogenized by adding three times the amount of STM buffer. After the homogenate was centrifuged at 10,000 x g for 10 minutes, the supernatant was homogenized once more to prepare an enzyme solution. The plant extract of Example 1 was added to phosphate buffer (pH6.5), 500 μM testosterone, and 770 μM NADPH (nicotinamide adenine dinucleotide phosphate). Next, the prepared enzyme solution was added and reacted at 37° C. for 30 minutes. After adding dichloromethane to terminate the reaction, centrifugation was performed at 2,000 rpm for 10 minutes. After separating and concentrating the dichloromethane layer, it was dissolved in 50% methanol and quantified using high-speed chromatography. 5α-reductase inhibitory ability was calculated by the formula of 5α-reductase inhibitory ability (%) = [1 - (area value of sample - area value of blank sample) / (area value of test group - area value of blank sample)] × 100 .

As shown in Figure 1, it was confirmed that the clownfish extract was treated with 125, 250 or 500 μg/ml, and exhibited 5α-reductase inhibitory activity in a concentration-dependent manner. These results indicate that the H. clownfish extract is effective in inhibiting or improving hair loss.

Experimental Example 2: Confirmation of cell proliferation and cytotoxicity

It was confirmed what effect the extracts of Kogwangnamu, Hsagi chinensis, or Arabidopsis obtained in Example 1 had on cell proliferation and cytotoxicity on human follicle dermal papilla cells (HFDPC), which are dermal papilla cells.

HFDPC cells were cultured in Follicle Dermal Papilla Cell Growth Medium containing a supplement mix (5% fetal calf serum, 0.5% bovine pituitary extract, 0.5 μg basic fibroblast growth factor, 2.5 mg insulin) and 1% antibiotic antimycotic solution. The HFDPC cell line was dispensed into a 96-well plate at a concentration of 1.0 X 10 ⁴ cells/well and cultured for 24 hours in a CO ₂ incubator controlled at 37° C., 95% humidity, and 5% CO ₂ . Afterwards, the medium was replaced with a medium without a supplement mix, and samples of each concentration were treated and cultured for 24 hours, and the degree of cell viability was measured by MTT assay. The MTT assay is a method for measuring the amount of formazan formed from reduced MTT by mitochondria of living cells. More specifically, each cell was treated with 100 µl of 0.5 mg/ml MTT solution and incubated for 4 hours, then the solution was completely removed and the absorbance of the plate dissolved in DMSO was measured at 540 nm.

As shown in Figure 2, it was confirmed that there was no cytotoxicity at all treatment concentrations, and treated with the extract of Kogwang tree, the extract of Clownfish extract, and the extract of Arabidopsis at each concentration. In particular, it was confirmed that the Gogwang tree extract significantly increased cell growth at a concentration of 25 to 50 μg/ml and the extract of Arabidopsis at a concentration of 50 to 100 μg/ml. These results indicate that the extract of Kogwang tree and the extract of Arabidopsis are effective for hair growth.

Experimental Example 3: Confirmation of suppression of cell destruction-related gene expression

Changes in cell destruction-related gene expression in dermal papilla cells (HFDPC) were confirmed with respect to the extracts of Gogwangnamu, Hsagi chinensis, or Arabidopsis obtained in Example 1.

Specifically, HFDPC cells were cultured with Follicle Dermal Papilla Cell Growth Medium containing supplement mix (5% fetal calf serum, 0.5% bovine pituitary extract, 0.5 μg basic fibroblast growth factor, 2.5 mg insulin) and 1% antibiotic antimycotic solution. The HFDPC cell line was dispensed into a 6-well plate at a concentration of 2.0 X 10 ⁵ cells/well and cultured for 24 hours in a CO ₂ incubator controlled at 37° C., 95% humidity, and 5% CO ₂ . Then, after replacing the medium with no supplement mix, 50 μM of DHT was treated to induce cell destruction. Then, the extracts of Example 1 were treated and cultured for 24 hours. After that, RNA was extracted from the obtained cells using Trizol reagent, cDNA was synthesized from the obtained RNA using a cDNA synthesis kit (Bio-Rad), and quantitative real time-PCR was performed using the synthesized cDNA as a template. PCR, qRT-PCR) was performed. The RT-PCR reaction was performed under conditions of 95°C for 20 seconds, 95°C for 3 seconds, and 72°C for 30 seconds for 40 cycles. The expression level of the gene was finally analyzed through correction for the GAPDH gene. qRT-PCR was performed using the oligomers shown in Table 1 as primers.

gene primer direction Primer sequence (5'→3') AR Forward CCC ACT TGT GTC AAA AGC GA Reverse AGG TCT TCT GGG GTG GAA AG DKK-1 Forward TCG GTT CTC AAT TCC AAC GC Reverse CGG CTG GTA GTT GTC AAT GG TGF-β1 Forward CTG GCG ATA CCT CAG CAA CC Reverse CGG TAG TGA ACC CGT TGA TGT C GAPDH Forward GGA GCG AGA TCC CTC CAA AAT Reverse GGC TGT TGT CAT ACT TCT CAT GG

As shown in FIG. 3, it was confirmed that the mRNA of the androgen receptor (AR), which was increased by dihydrotestosterone (DHT), decreased significantly in a concentration-dependent manner when the extract of Kogwang tree extract, the extract of Clownfish extract, or the extract of Arabidopsis was treated. In particular, it was confirmed that all the extracts of Example 1 inhibited androgen receptor gene expression more effectively than finasteride used as a positive control.

Figure 4 is a graph confirming the mRNA change of Dickkopf-1 (DKK-1) upon treatment with the extract of Example 1. As shown in FIG. 4, it was confirmed that the expression of DKK-1, a cell destruction-related factor, which was increased by DHT treatment, was significantly reduced by the extract of Clownfish extract and the extract of Arabidopsis.

Figure 5 is a graph confirming the mRNA change of transforming growth factor beta 1 (TGF-β1) upon treatment of the extract of Example 1, it was confirmed that the expression of TGF-β1 was significantly reduced in all extracts.

The above results indicate that the extracts of Kogwang tree extract, extract of clown clover, and extract of Arabidopsis japonica are effective in improving scalp and hair health by suppressing the expression of genes related to cell destruction.

Experimental Example 4: Confirmation of promotion of cell proliferation-related gene expression

Changes in cell proliferation-related gene expression in dermal papilla cells (HFDPC) were confirmed with respect to the extracts of Kogwangnamu, Hsagi chinensis or Aegis suyeong obtained in Example 1.

Specifically, HFDPC cells were cultured with Follicle Dermal Papilla Cell Growth Medium containing supplement mix (5% fetal calf serum, 0.5% bovine pituitary extract, 0.5 μg basic fibroblast growth factor, 2.5 mg insulin) and 1% antibiotic antimycotic solution. The HFDPC cell line was dispensed into a 6-well plate at a concentration of 2.0 X 10 ⁵ cells/well and cultured for 24 hours in a CO ₂ incubator controlled at 37° C., 95% humidity, and 5% CO ₂ . Then, after replacing the medium with no supplement mix, the extracts of Example 1 were treated and cultured for 24 hours. After that, RNA was extracted from the obtained cells using Trizol reagent, cDNA was synthesized from the obtained RNA using a cDNA synthesis kit (Bio-Rad), and quantitative real time-PCR was performed using the synthesized cDNA as a template. PCR, qRT-PCR) was performed. The RT-PCR reaction was performed under conditions of 95°C for 20 seconds, 95°C for 3 seconds, and 72°C for 30 seconds for 40 cycles. The expression level of the gene was finally analyzed through correction for the GAPDH gene. qRT-PCR was performed using the oligomers shown in Table 2 as primers.

gene primer direction Primer sequence (5'→3') IGF-1 Forward GGG GAC TTT CGT GAC TGA GC Reverse GGT AGG TCC GGG TCG TTT AC LEF1 Forward AAT GAG AGC GAA TGT CGT TGC Reverse GCT GTC TTT CTT TCC GTG CTA BMP 2 Forward TGC ACC AAG ATG AAC ACA GC Reverse CGC TGT TTG TGT TTG GCT TG GAPDH Forward GGA GCG AGA TCC CTC CAA AAT Reverse GGC TGT TGT CAT ACT TCT CAT GG

As shown in FIG. 6, it was confirmed that the mRNA of insulin like growth factor-I (IGF-1) increased in a concentration-dependent manner when treated with the clownfish extract or the A.

As shown in Figure 7, all extracts of Example 1 significantly increased the gene expression of Lymphoid Enhancer Binding Factor 1 (LEF1).

In addition, Figure 8 is the result of confirming the mRNA expression of Bone morphogenetic protein 2 (BMP2) when the extract of Example 1 was treated, and the gene expression of BMP2 increased in a concentration-dependent manner when the extract of Gogwang tree and the extract of Arabidopsis were treated. I was able to confirm that

The above results indicate that the extracts of Kogwangnamu extract, extracts of Hsagi chinensis, and extracts of Arabidopsis have an effect on improving scalp and hair health by increasing cell proliferation.

Formulation Example 1: Preparation of tablets

For Example 1, tablets were prepared by mixing the ingredients in Table 3 below and tableting according to a conventional tablet manufacturing method.

raw material name unit weight (mg) Example 1 10.0000 silicon dioxide 15.3000 Magnesium Stearate 10.8000 crystalline cellulose 799.4951 Hydroxypropyl Methyl Cellulose 29.0700 Carboxymethyl Cellulose Calcium 27.0000 Glycerin fatty acid ester 0.6930 titanium dioxide 1.4697 red national pigment 4.4082 powdered caramel color 1.7640

Formulation Example 2: Preparation of capsules

In Example 1, soft capsules were prepared by mixing the ingredients in Table 4 below and filling them into gelatin capsules according to the usual capsule preparation method.

raw material name unit weight (mg) Example 1 10 vitamin E 2.25 vitamin C 2.25 palm oil 0.5 hydrogenated vegetable oil 2 Yellow lead One lecithin 2.25 Soft capsule filling solution 427.75

Formulation Example 3: Preparation of liquid formulation

According to the beverage manufacturing method suitable for the taste with respect to Example 1, the ingredients in Table 5 were mixed and filled in a bottle or pouch to prepare a liquid formulation.

raw material name unit weight (g) Example 1 0.5050 xanthan gum 0.0075 fructooligosaccharide solution 0.7500 Coconut Flower Extract Powder 1.0500 Ssanghwa Concentrate 1.5000 Red Ginseng Fragrance 0.0450 Purified water 11.1425

Formulation Example 4: Preparation of Jelly

With respect to Example 1, according to the jelly manufacturing method suitable for the taste, the ingredients in Table 6 were mixed and filled in a three-sided cloth to prepare a jelly.

raw material name unit weight (g) Example 1 0.5000 food gel 0.3600 carrageenan 0.0600 calcium lactate 0.1000 sodium citrate 0.0600 complex gold extract 0.0200 Enzymatically Treated Stevia 0.0440 fructooligosaccharide solution 5.0000 Red Grape Concentrate 2.4000 Purified water 15.4560

Although the above composition ratio is generally prepared as a formulation example by mixing suitable ingredients, the mixing ratio and raw materials may be arbitrarily changed as necessary.

Since the extract of the present invention or the compounds derived therefrom are stable under all test conditions of the formulation examples, there was no problem with the stability of the formulation.

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Claims (12)

A health functional food composition for improving hair or scalp condition comprising at least one selected from the group consisting of Philadephus schrenckii extract, Securinega suffruticosa extract and Rumex acetosella extract as an active ingredient. The health functional food composition of claim 1, wherein the extract is extracted with water, C1 to C4 alcohol, or a mixed solvent thereof. The method according to claim 1, wherein the extract is contained in 0.0001 to 90% by weight relative to the total weight of the composition, the health functional food composition for improving hair or scalp condition. The method according to claim 1, wherein the hair or scalp condition improvement is to prevent or improve hair damage; promote hair growth; Or hair loss suppression, prevention or improvement of the functional food composition for improving hair or scalp conditions. The method according to claim 1, wherein the extract inhibits 5α-reductase (5α-reductase) and promotes the proliferation of dermal papilla cells, health functional food composition for improving hair or scalp conditions. The method according to claim 1, wherein the extract reduces the expression of one or more selected from the group consisting of androgen receptor (AR), Dickkopf-1 (DKK-1) and Transforming Growth Factor Beta 1 (TGF-β1), Health functional food composition for improving hair or scalp condition. The method according to claim 1, wherein the extract increases the expression of one or more selected from the group consisting of Insulin like growth factor-I (IGF-1), Lymphoid Enhancer Binding Factor 1 (LEF1) and Bone morphogenetic protein 2 (BMP2), Health functional food composition for improving hair or scalp condition. The method according to claim 1, wherein the health functional food has a formulation selected from the group consisting of powders, granules, tablets, capsules, pills, gels, jellies, suspensions, emulsions, syrups, tea bags, leaching teas and health drinks, Health functional food composition for improving hair or scalp condition. A cosmetic composition for improving hair or scalp condition comprising at least one selected from the group consisting of Philadephus schrenckii extract, Securinega suffruticosa extract and Rumex acetosella extract as an active ingredient. The method according to claim 9, wherein the hair or scalp condition improvement is hair damage prevention or improvement; promote hair growth; Or hair loss suppression, prevention or improvement of the cosmetic composition for improving hair or scalp conditions. A pharmaceutical composition for preventing or treating hair loss comprising at least one selected from the group consisting of Philadephus schrenckii extract, Securinega suffruticosa extract, and Rumex acetosella extract as an active ingredient. An external skin preparation for improving hair or scalp conditions comprising at least one selected from the group consisting of Philadephus schrenckii extract, Securinega suffruticosa extract, and Rumex acetosella extract as an active ingredient.

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