KR20230086837A - Buffer dispensing apparatus for the preparation of solid sample - Google Patents

Abstract

The present invention relates to a solvent supply device for pretreatment of a solid sample, and the solvent supply device for pretreatment of a solid sample according to the present invention includes a grinding container having a receiving space communicating with an external space through an opening; a crushing pestle inserted into the accommodation space through the opening and pulverizing the solid sample accommodated in the accommodation space; and a buffer solution supply unit supplying the buffer solution to the solid sample accommodated in the accommodation space of the grinding container, wherein the buffer solution is provided in the form of fine droplets or droplets.

Description

Solvent supply apparatus for the preparation of solid samples {Buffer dispensing apparatus for the preparation of solid sample}

The present invention relates to a solvent supply device for pretreatment of a solid sample, and more particularly, to supply a small amount of a buffer solution in the form of fine droplets or droplets, etc. A solvent supply device for pretreatment of a solid sample capable of minimizing dilution of a target substance.

In order to detect harmful substances present in solid samples such as food, fruit trees, and other plant resources, processes such as liquefaction, homogenization, juice extraction, and dilution are required to liquefy the sample in a form suitable for the analysis / inspection method.

For the grinding and homogenization process, a stomacher method, a ball milling method, a grinding method using a crucible and a pestle, and the like are used.

The stomacher method, as in Korean Patent Publication No. 10-2013-0104328, puts the sample and buffer solution inside the plastic bag, and then blows the plastic bag containing the sample in a solid state through a paddle. By doing so, the sample is finely crushed. Through this, the particle size of the sample is reduced and the particle shape and viscosity are homogenized, and the soft tissue is formed so that the substance inside is released to the outside, so that the substance to be detected inside the solid sample can be eluted in the form of a liquid sample.

The ball milling method is to pulverize/homogenize a sample by using collision/friction between the steel bead and the sample generated during reciprocating and rotational motion after a sample, a buffer solution, and steel beads are put into a grinding container.

As in Korean Patent Registration No. 10-2177634, the pulverization method using the crucible and pestle is to physically pulverize the sample using a pestle after putting a sample and a buffer solution into a crucible.

However, since most of these methods perform grinding and homogenization after pre-injecting a large amount of buffer solution, there is a problem in that the concentration of the substance to be detected in the extracted liquid sample is low and the amount of the sample is wasted.

In addition, as the concentration of the substance to be detected is lowered due to the introduction of the buffer solution, there is a problem in that an additional sample concentration process or expensive and more sophisticated analysis/inspection technology is required to secure the reliability of the analysis/inspection result.

Republic of Korea Patent Publication No. 10-2013-0104328 Republic of Korea Patent No. 10-2177634

Therefore, an object of the present invention is to solve this conventional problem, by supplying a small amount of a buffer solution in the form of droplets or droplets, etc. An object of the present invention is to provide a solvent supply device for pretreatment of a solid sample capable of minimizing dilution of a substance.

The above object, according to the present invention, the crushing container formed with a receiving space communicating with the external space through the opening; a crushing pestle inserted into the accommodation space through the opening and pulverizing the solid sample accommodated in the accommodation space; and a buffer solution supply unit supplying a buffer solution to the solid sample accommodated in the receiving space of the grinding vessel, wherein the buffer solution is provided in the form of fine droplets or droplets and is achieved by a solvent supply device for pretreatment of the solid sample.

Here, it is preferable that the buffer solution supply unit supplies the buffer solution in the form of fine droplets to the solid sample in the accommodation space through micropores formed in the grinding container.

In addition, the buffer solution supply unit preferably includes a cover covering an outer surface of the grinding container to form a supply passage between the grinding container and an inlet for supplying the buffer solution to the supply passage.

In addition, it is preferable that the droplet size and discharge amount of the buffer solution are controlled by the injection pressure of the buffer solution injected through the inlet.

In addition, the micropores are preferably disposed at positions corresponding to the pulverization area in which the sample is pulverized.

In addition, it is preferable that the buffer solution supply unit provides a buffer solution in the form of fine droplets into the accommodation space through an opening of the grinding container.

In addition, the buffer solution supply unit has a supply passage formed therein and a cover assembled in the form of closing the opening of the grinding container, an inlet for supplying the buffer solution to the supply passage, and a surface facing the receiving space of the cover. It is preferable to include a nozzle hole disposed and discharging a buffer solution.

In addition, the cover preferably includes a through hole through which the crushing pestle can pass.

In addition, it is preferable that a plurality of micropores through which the buffer solution in the form of fine droplets can be discharged to the outside of the grinding vessel are formed in the grinding vessel.

In addition, the grinding vessel preferably includes a hydrophobic material surface layer formed on an inner wall surface of the micropores so that the liquefied sample pulverized in the accommodation space does not flow out of the grinding vessel through the micropores.

In addition, the micropores are preferably disposed at positions corresponding to the pulverization area in which the sample is pulverized.

In addition, the grinding pestle includes a rod portion and a grinding head portion disposed at an end of the rod portion and having a diameter gradually decreasing as the distance from the rod portion increases, and the grinding area corresponding to the grinding head portion of the accommodation space is It is preferable to have a shape in which the diameter gradually decreases corresponding to the shape of the crushing head.

In addition, it is preferable that a spiral groove is formed on the outer circumferential surface of the grinding head unit to move the liquefied sample pulverized between the grinding vessel and the grinding container toward the rod portion when the grinding head unit rotates.

In addition, it is preferable that the connecting region connecting the opening of the receiving space and the crushing region has a cylindrical shape having a diameter corresponding to the largest diameter portion of the crushing head.

According to the present invention, by supplying a small amount of a buffer solution in the form of fine droplets or droplets, which is put together with the sample into the grinding container during the sample homogenization process, solid sample pretreatment that can minimize the dilution of the detection target material during the homogenization process A solvent supply device is provided.

1 is a perspective view of a solvent supply device for pretreatment of a solid sample according to a first embodiment of the present invention;
Figure 2 is an exploded perspective view of Figure 1;
3 is a cross-sectional view of a solvent supply device for pretreatment of a solid sample according to a first embodiment of the present invention;
4 is a functional diagram of a solvent supply device for pretreatment of a solid sample according to a first embodiment of the present invention;
5 is a perspective view of a solvent supply device for pretreatment of a solid sample according to a second embodiment of the present invention;
Figure 6 is an exploded perspective view of Figure 5;
7 is a cross-sectional view of a solvent supply device for pretreatment of a solid sample according to a second embodiment of the present invention;
8 is a functional diagram of a solvent supply device for pretreatment of a solid sample according to a second embodiment of the present invention.

Prior to description, in various embodiments, components having the same configuration are typically described in the first embodiment using the same reference numerals, and in other embodiments, components different from those of the first embodiment will be described. do.

Hereinafter, a solvent supply device for pretreatment of a solid sample according to a first embodiment of the present invention will be described in detail with reference to the accompanying drawings.

Among the accompanying drawings, FIG. 1 is a perspective view of a solvent supply device for pre-treatment of solid samples according to a first embodiment of the present invention, FIG. 2 is an exploded perspective view of FIG. 1, and FIG. 3 is a pre-treatment of solid samples according to a first embodiment of the present invention. It is a cross-sectional view of the solvent supply device.

As shown in the drawings, the solvent supply device for pretreatment of a solid sample according to the first embodiment of the present invention includes a grinding container 110, a grinding pestle 120, and a buffer solution supply unit 130.

The crushing vessel 110 has an accommodation space 111 formed therein, and an opening connecting the accommodation space 111 and an external space is formed on the upper side.

The accommodating space 111 includes a grinding area 111a where the sample S is pulverized by the pulverizing head 122 of the pulverizing pestle 120 and the pulverizing head 122 of the pulverizing pestle 120. It may be divided into a connection area 111b connecting the grinding area 111a and the opening so that the grinding area 111a can be entered through the opening. Here, the crushing area 111a may have a shape gradually narrowing as it moves away from the opening, and the connecting area 111b may have a cylindrical shape having a constant diameter along the longitudinal direction.

In addition, a plurality of micropores 112 capable of supplying the buffer solution (B) into the grinding area 111a are formed through a region corresponding to the grinding area 111a of the grinding container 110, and the micropores The diameter of 112 is preferably set to a size that allows the buffer solution (B) to be discharged in the form of fine droplets.

The crushing pestle 120 is for crushing the solid sample S accommodated in the accommodating space 111, and can rotate by an external force while being inserted into the accommodating space 111 of the crushing vessel 110. The rod portion 121 has a larger volume than the rod portion 121, and the sample S is pulverized while rubbing against the inner wall surface of the pulverizing container 110 in the pulverizing area 111a of the receiving space 111. It includes a crushing head 122 disposed at the distal end of the rod portion 121 so as to be able to do so. Corresponding to the shape of the crushing area 111a, the crushing head 122 has a shape in which the diameter gradually decreases as it moves away from the rod part 121, and the crushing container 110 rotates on the outer circumferential surface. Spiral grooves 123 and 123 for moving the pulverized liquefied sample S' to the rod portion 121 side are formed between and.

The buffer solution supply unit 130 serves to supply the buffer solution B to the grinding area 111a of the receiving space 111, and forms a supply passage between the outer surface of the grinding container 110. It includes a cover 131 watertightly covering an outer surface of the grinding container 110 except for the opening and an inlet 132 for supplying the buffer solution B to the supply passage.

On the other hand, the inlet 132 may be connected to a storage tank for storing the buffer solution (B), and between the storage tank and the inlet 132, a pump capable of providing pressure for supply of the buffer solution (B) can be provided.

The buffer solution (B) supplied to the supply passage through the inlet 132 is discharged in the form of fine droplets through the micropores 112 formed in the grinding container 110 by the supply pressure of the buffer solution (B). The droplet size and discharge amount of the buffer solution (B) may be controlled by the supply pressure of the buffer solution (B) supplied through the inlet 132.

Now, the operation of the first embodiment of the above-described solvent supply device for pretreatment of a solid sample will be described.

4 of the accompanying drawings is a functional diagram of a solvent supply device for pretreatment of a solid sample according to a first embodiment of the present invention.

As shown in FIG. 4, looking at the grinding process of the solid sample S using the grinding container 110 and the grinding pestle 120 according to the present embodiment, the receiving space 111 through the opening of the grinding container 110 ) After inserting the sample (S) into the inside, inserting the pulverizing head part 122 of the pulverizing pestle 120 into the pulverizing area 111a at the bottom of the receiving space 111, and then pivoting the rod part 121, The sample S may be pulverized/homogenized using pressure and friction between the pulverizing head 122 and the pulverizing container 110 .

At this time, the inlet 132 formed on one side of the cover 131 by the cover 131 of the buffer solution supply unit 130 surrounding the outside of the grinding container 110 and the fine pores 112 formed in the grinding container 110 ) A supply passage is formed between. Therefore, when the buffer solution (B) is supplied at a predetermined pressure through the inlet 132, the buffer solution (B) flows along the supply passage to the area corresponding to the grinding area (111a) of the grinding container (110). It is discharged in the form of fine droplets through the formed micropores 112 . In this way, when the buffer solution (B) is directly supplied to the grinding area 111a where the sample S is pulverized, that is, the contact portion between the pulverizing container 110 and the pulverizing head 122, the pulverization of the sample S is performed. Since the buffer solution (B) can be prevented from being unnecessarily supplied to the remaining areas, the supply amount of the buffer solution (B) used in the grinding/homogenization process of the solid specimen can be minimized.

In addition, since the droplet size and discharge amount of the buffer solution (B) can be controlled by the supply pressure of the buffer solution (B) supplied through the inlet 132, the buffer solution The supply of (B) can be optimized.

In addition, the grinding area 111a of the grinding vessel 110 has a shape gradually narrowing as it proceeds in a direction away from the opening, and the grinding head 122 corresponds to the shape of the grinding area 111a. Since it has a shape in which the diameter gradually decreases as it moves away from the rod portion 121, the outer surface of the grinding head 122 is formed on the inner wall surface of the grinding container 110 in the grinding area 111a. can be attached. Therefore, the liquefied sample (S′) pulverized between the pulverizing container 110 and the pulverizing head 122 rises along the spiral groove 123 formed on the outer surface of the pulverizing head 122, and then rises to the pulverizing head. It can be moved to the upper area of (122).

According to this embodiment, the supply amount of the buffer solution (B) can be minimized by supplying a small amount of the buffer solution (B) in the form of fine droplets in the process of homogenizing the solid sample (S), and the liquefied sample after grinding has been completed Since (S′) can be moved to the upper region of the grinding head 122 to separate it from the buffer solution (B) supplied to the grinding area 111a, dilution of the substance to be detected during the homogenization process can be minimized. .

Next, a solvent supply device for pretreatment of a solid sample according to a second embodiment of the present invention will be described.

Among the accompanying drawings, FIG. 5 is a perspective view of a solvent supply device for pretreatment of solid samples according to a second embodiment of the present invention, FIG. 6 is an exploded perspective view of FIG. 5, and FIG. 7 is a pretreatment of solid samples according to a second embodiment of the present invention. 8 is a cross-sectional view of a solvent supply device for pretreatment of a solid sample according to a second embodiment of the present invention.

As shown in the drawings, in the solvent supply device for pretreatment of solid samples according to the second embodiment of the present invention, the buffer solution supply unit 130' is disposed in the upper opening of the grinding container 110, and the buffer solution supply unit (130') has a configuration difference from the above-described first embodiment in that the buffer solution (B) is provided in the form of fine droplets.

The buffer solution supply unit 130 is a cover 131 assembled to the opening of the grinding container 110 and having a supply passage formed therein, and one side of the cover 131 to supply the buffer solution B to the supply passage. An inlet 132 formed in the cover 131, a plurality of nozzle holes 133 formed on the surface facing the receiving space 111 and communicating with the supply passage, and the crushing pestle 120 are the cover ( 131) and a through hole 134 formed through the center of the cover 131 so that it can be installed in a state passing through, and the buffer solution (B) can be provided in the form of fine droplets to the injection hole 132 A sprayer or mister may be connected.

Meanwhile, since the rest of the configuration except for the buffer solution supply unit 130 is the same as that of the first embodiment, a detailed description of the same configuration will be omitted.

According to the configuration as described above, in a state in which the opening of the grinding vessel 110 is closed using the cover 131' of the buffer solution supply unit 130', the buffer solution in the form of fine droplets ( When B) is supplied at a predetermined pressure, the buffer solution (B) in the form of fine droplets passes through the supply passage inside the cover 131 'through a plurality of nozzle holes 133 to the accommodation space of the grinding container 110 ( 111) can be provided.

Specifically, as shown in FIG. 8, in a state in which the solid sample S and the grinding head 122 of the grinding pestle 120 are inserted into the receiving space 111 of the grinding container 110, the buffer solution supply unit 130' Assemble the cover 131 'of) to the opening of the grinding container 110. At this time, the rod portion 121 of the crushing pestle 120 is exposed to the outside through a through hole 134 formed in the center of the cover 131', and between the through hole 134 and the rod portion 121 The assembly surface of may be sealed by a sealing member or the like.

Subsequently, when the buffer solution (B) in the form of fine droplets is supplied to the inlet 132 of the buffer solution supply unit 130, it is supplied into the accommodation space 111 through the nozzle hole 133, and is provided within the accommodation space 111. The supplied buffer solution (B) in the form of fine droplets can maintain a state of being filled at a constant pressure inside the accommodation space 111 by the supplied pressure.

In this state, when the grinding pestle 120 is rotated to grind the solid sample S located between the grinding head 122 and the grinding container 110, the buffer solution in the form of fine droplets supplied into the receiving space 111 ( As B) is condensed on the surface of the solid sample (S), a small amount of the buffer solution (B) may be supplied to the sample (S).

Meanwhile, the buffer solution (B) in the form of fine droplets not supplied to the grinding process of the sample (S) is discharged to the outside of the grinding vessel 110 through the micropores 112 formed in the grinding vessel 110.

That is, the buffer solution (B) provided in the receiving space 111 exists in the form of fine droplets and is condensed on the surface of the solid sample (S) so that it can be directly supplied to the sample (S), and not supplied to the sample (S). Since the remaining buffer solution (B) can be discharged to the outside through the micropores 112 of the grinding container 110, the amount of supply of the buffer solution (B) applied to the specimen during grinding/homogenization of the solid specimen can be minimized. there is. As a result, it is possible to minimize the dilution of the target substance to be detected during the homogenization process of the liquefied sample (S'), enabling more reliable analysis/inspection, as well as reducing the use of reagents and omitting the additional sample (S) concentration process. The pretreatment process of (S) can be simplified.

On the other hand, the grinding vessel 110 may be made of a hydrophobic material, such as the periphery and inner wall surface of the micropores 112 made of a hydrophobic material surface layer, or may include a hydrophobic material surface layer. Accordingly, the grinding container 110 discharges the buffer solution B in the form of extra fine droplets filled in the receiving space 111 to the outer space of the grinding container 110 through the micropores 112, and crushing. It is possible to prevent the liquefied sample S' pulverized by the pestle 120 from leaking out of the pulverizing container 110 through the micropores 112 .

The scope of the present invention is not limited to the above-described embodiments, but may be implemented in various forms of embodiments within the scope of the appended claims. Anyone with ordinary knowledge in the art to which the invention pertains without departing from the subject matter of the invention claimed in the claims is considered to be within the scope of the claims of the present invention to various extents that can be modified.

110: grinding container, 111: receiving space, 111a: grinding area,
111b: connection area, 112: micropores, 120: crushing pestle,
121: rod part, 122: grinding head part, 123: spiral groove,
130: buffer solution supply unit, 131: cover, 132: inlet,
133: nozzle hole, 134: through hole, S: sample,
S': liquefaction sample, B: buffer solution

Claims (14)

A crushing container having an accommodation space communicating with an external space through an opening;
a crushing pestle inserted into the accommodation space through the opening and pulverizing the solid sample accommodated in the accommodation space; and
A buffer solution supply unit for supplying a buffer solution to the solid sample accommodated in the receiving space of the grinding container; includes,
The buffer solution is a solvent supply device for pretreatment of solid samples provided in the form of fine droplets or droplets. According to claim 1,
The buffer solution supply unit supplies a buffer solution in the form of fine droplets to the solid sample in the accommodation space through micropores formed in the grinding container. According to claim 2,
The buffer solution supply unit includes a cover surrounding an outer surface of the grinding container to form a supply passage between the grinding container and an inlet for supplying the buffer solution to the supply passage. According to claim 3,
A solvent supply device for pretreatment of a solid sample in which the droplet size and discharge amount of the buffer solution are controlled by the injection pressure of the buffer solution injected through the inlet. According to claim 2,
The micropore is a solvent supply device for pretreatment of a solid sample disposed at a position corresponding to a pulverization area in which the pulverization of the sample is performed. According to claim 1,
The solvent supply device for pre-processing solid samples, wherein the buffer solution supply unit provides a buffer solution in the form of fine droplets into the accommodation space through an opening of the grinding container. According to claim 6,
The buffer solution supply unit has a supply passage formed therein and is disposed on a cover assembled in a form to close the opening of the grinding container, an inlet for supplying a buffer solution to the supply passage, and a surface of the cover facing the receiving space, A solvent supply device for pretreatment of a solid sample comprising a nozzle hole for discharging a buffer solution. According to claim 7,
The cover is a solvent supply device for pretreatment of solid samples including a through hole through which the grinding pestle passes. According to claim 6,
A solvent supply device for pretreatment of solid samples in which a plurality of micropores are formed in the grinding vessel to discharge the buffer solution in the form of fine droplets to the outside of the grinding vessel. According to claim 9,
The grinding vessel includes a hydrophobic material surface layer formed on an inner wall surface of the micropores so that the liquefied sample pulverized in the accommodation space does not flow out of the grinding vessel through the micropores Solvent supply device for pretreatment of solid samples. According to claim 10,
The micropore is a solvent supply device for pretreatment of a solid sample disposed at a position corresponding to a pulverization area in which the pulverization of the sample is performed. According to any one of claims 1 to 11,
The crushing pestle includes a rod portion and a grinding head portion disposed at an end of the rod portion and gradually decreasing in diameter as the distance from the rod portion increases,
The solvent supply device for preprocessing solid samples having a shape in which a diameter of the grinding area corresponding to the grinding head portion of the accommodation space gradually decreases corresponding to the shape of the grinding head portion. According to claim 12,
A solvent supply device for pretreatment of solid samples in which a spiral groove is formed on the outer circumferential surface of the grinding head to move the liquefied sample pulverized between the grinding container and the grinding container toward the rod portion when the grinding head is rotated. According to claim 13,
The connection region connecting the opening of the receiving space and the grinding region has a cylindrical shape having a diameter corresponding to the largest diameter portion of the grinding head unit.

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