KR20230078065A - Food composition for flavor improvement containing fermented mixture of Astragalus membranaceus and Lentinula edodes as effective component - Google Patents
Food composition for flavor improvement containing fermented mixture of Astragalus membranaceus and Lentinula edodes as effective component Download PDFInfo
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- KR20230078065A KR20230078065A KR1020210165459A KR20210165459A KR20230078065A KR 20230078065 A KR20230078065 A KR 20230078065A KR 1020210165459 A KR1020210165459 A KR 1020210165459A KR 20210165459 A KR20210165459 A KR 20210165459A KR 20230078065 A KR20230078065 A KR 20230078065A
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- astragalus
- mixed
- paste
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- 240000000599 Lentinula edodes Species 0.000 title claims abstract description 55
- 239000000203 mixture Substances 0.000 title claims abstract description 29
- 239000000796 flavoring agent Substances 0.000 title claims abstract description 21
- 235000019634 flavors Nutrition 0.000 title claims abstract description 20
- 235000013305 food Nutrition 0.000 title claims abstract description 20
- 235000001715 Lentinula edodes Nutrition 0.000 title claims abstract description 11
- 235000006533 astragalus Nutrition 0.000 title claims description 50
- 241000045403 Astragalus propinquus Species 0.000 title abstract description 4
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 40
- 244000068988 Glycine max Species 0.000 claims abstract description 40
- 239000004480 active ingredient Substances 0.000 claims abstract description 29
- 240000004160 Capsicum annuum Species 0.000 claims abstract description 23
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 claims abstract description 23
- 235000007862 Capsicum baccatum Nutrition 0.000 claims abstract description 23
- 239000001728 capsicum frutescens Substances 0.000 claims abstract description 23
- 241001061264 Astragalus Species 0.000 claims description 48
- 210000004233 talus Anatomy 0.000 claims description 48
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- 150000001413 amino acids Chemical class 0.000 abstract description 57
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 abstract description 22
- 235000013922 glutamic acid Nutrition 0.000 abstract description 22
- 239000004220 glutamic acid Substances 0.000 abstract description 22
- 235000019640 taste Nutrition 0.000 abstract description 13
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- 230000000694 effects Effects 0.000 abstract description 4
- 235000019600 saltiness Nutrition 0.000 abstract description 2
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 29
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- 235000015429 Mirabilis expansa Nutrition 0.000 description 7
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- WZFUQSJFWNHZHM-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)CC(=O)N1CC2=C(CC1)NN=N2 WZFUQSJFWNHZHM-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 241000353355 Oreosoma atlanticum Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 244000000231 Sesamum indicum Species 0.000 description 2
- 235000003434 Sesamum indicum Nutrition 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 244000273928 Zingiber officinale Species 0.000 description 2
- 235000006886 Zingiber officinale Nutrition 0.000 description 2
- 229940107666 astragalus root Drugs 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
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- 239000012895 dilution Substances 0.000 description 2
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 2
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- 230000014759 maintenance of location Effects 0.000 description 2
- 235000020429 malt syrup Nutrition 0.000 description 2
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
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- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000008159 sesame oil Substances 0.000 description 2
- 235000011803 sesame oil Nutrition 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 235000019605 sweet taste sensations Nutrition 0.000 description 2
- 235000020357 syrup Nutrition 0.000 description 2
- 239000006188 syrup Substances 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- YLZOPXRUQYQQID-UHFFFAOYSA-N 3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]propan-1-one Chemical compound N1N=NC=2CN(CCC=21)CCC(=O)N1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F YLZOPXRUQYQQID-UHFFFAOYSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000512259 Ascophyllum nodosum Species 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241000237519 Bivalvia Species 0.000 description 1
- 241000238097 Callinectes sapidus Species 0.000 description 1
- 241001507683 Penicillium aurantiogriseum Species 0.000 description 1
- 229910021607 Silver chloride Inorganic materials 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000019256 formaldehyde Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- RQFCJASXJCIDSX-UUOKFMHZSA-N guanosine 5'-monophosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O RQFCJASXJCIDSX-UUOKFMHZSA-N 0.000 description 1
- 235000013928 guanylic acid Nutrition 0.000 description 1
- 239000004226 guanylic acid Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000014594 pastries Nutrition 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000021067 refined food Nutrition 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 1
- 235000019614 sour taste Nutrition 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/70—Germinated pulse products, e.g. from soy bean sprouts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/30—Removing undesirable substances, e.g. bitter substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/50—Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/88—Taste or flavour enhancing agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/15—Flavour affecting agent
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/16—Taste affecting agent
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/208—Fungi extracts
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
Description
본 발명은 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 풍미개선을 위한 식품조성물에 관한 것으로, 더욱 상세하게는 관능적 품질에 영향을 주는 아미노태질소(amino nitrogen)의 양(量), 유리아미노산(free amino acid)의 양(量), 아미노산(amino acid)의 유리율(遊離率), 글루탐산(glutamic acid)의 양(量) 및 글루탐산의 유리율(遊離率)을 증가시킴으로써 짠맛 감소와 구수한 맛의 핵심인 감칠맛을 증가시키는 효능을 갖는 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 풍미개선을 위한 식품조성물에 관한 것이다.The present invention relates to a food composition for flavor improvement containing astragalus and shiitake mixed fermented product as an active ingredient, and more particularly, to the amount of amino nitrogen affecting sensory quality, glass By increasing the amount of free amino acid, the release rate of amino acid, the amount of glutamic acid, and the release rate of glutamic acid, It relates to a food composition for flavor improvement containing, as an active ingredient, a mixed fermented product of Astragalus and shiitake mushrooms, which has the effect of increasing umami, which is the core of savory taste.
된장은 우리나라 전통 발효 식품 중 하나로, 단백질을 공급하는 영양성과 저장성 측면에서 우수한 제품으로 알려져 있다. 서구화된 식문화, 가정간편식(HMR), 배달, 외식 등의 섭취 편의성이 중시되는 방향으로 식품시장이 빠르게 변화하면서 섭취시 부재료 첨가와 조리법의 번거로움이 있는 된장의 수요가 감소하였다(나혜진 외 2, 2020, 식품과학과 산업, 53(2) : 183-199). 다른 재료 추가 없이 간편하게 조리가 가능한 된장 제품에 대한 소비자 니즈(needs)가 증가함에 따라, 된장에 다시마, 바지락 및 꽃게 등의 부재료를 첨가해 풍미를 향상시킨 제품이 출시되고 있다. 풍미개선 된장 제품 시장은 16년 대비 17년에 40% 가량 상승하였기에 풍미개선 된장의 지속적인 개발이 필요하다(aT 한국농수산식품유통공사, 2017, 가공식품 마켓리포트 된장편, 2-5).Soybean paste is one of the traditional fermented foods in Korea and is known as an excellent product in terms of nutrition and storability to supply protein. As the food market is rapidly changing in the direction of focusing on westernized food culture, convenience of consumption such as home meal replacement (HMR), delivery, and eating out, the demand for soybean paste, which requires the addition of auxiliary ingredients and the hassle of cooking, has decreased (Na Hye-jin et al. 2, 2020, Food Science and Industry, 53(2): 183-199). As consumer needs for soybean paste products that can be easily cooked without adding other ingredients increase, products with improved flavor by adding sub-ingredients such as kelp, clams, and blue crabs to soybean paste are being released. The market for flavor-improved soybean paste products increased by 40% in 2017 compared to 2016, so continuous development of flavor-improved soybean paste is necessary (aT Korea Agro-Fisheries & Food Trade Corporation, 2017, Processed Food Market Report Soybean Paste, 2-5).
된장의 풍미개선에 대한 연구 또한 계속해서 진행되고 있다. 관련 특허로는 대한민국 공개특허공보 제10-2006-0040797호(발명의 명칭: 이취와 풍미가 개선된 장류의 제조방법)의 아스퍼질러스 오리제(Aspergillus oryzae)와 페니실리움 폴로니컴(Penicillium polonicum)을 사용한 이취와 풍미개선 장류 제조방법과 대한민국 공개특허공보 제10-2016-0126780호(발명의 명칭: 고로쇠 수액을 이용하여 풍미를 개선한 된장의 제조방법)의 고로쇠 수액을 사용한 된장 풍미개선이 있다.Research on improving the flavor of soybean paste is also ongoing. Related patents include Aspergillus oryzae and Penicillium polonicum of Korean Patent Publication No. 10-2006-0040797 (Title of Invention: Manufacturing Method of Paste with Improved Off-flavor and Flavor) ) and Korean Patent Laid-open Publication No. 10-2016-0126780 (Title of Invention: Method for Manufacturing Soybean Paste with Improved Flavor Using Gorosoe Sap) there is.
특히, 된장의 풍미 및 관능 향상에 주도적으로 관여하는 조건은 유리아미노산(free amino acid)의 양이 높음과 동시에 아미노산의 유리율이 높고, 지미성분인 글루탐산(glutamic acid)의 양과 글루탐산의 유리율이 증가하는 것이다(김종규, 2004, 한국식품위생안전성학회지, 19(1), 31-37).In particular, the conditions leading to the flavor and sensory enhancement of soybean paste are the high amount of free amino acid and high amino acid release rate, as well as the amount and release rate of glutamic acid, which is a flavor component. It is increasing (Kim Jong-gyu, 2004, Korean Journal of Food Hygiene and Safety, 19(1), 31-37).
황기(Astragalus membranaceus)는 단맛을 지니고 있으며, 황기를 첨가한 간장의 아미노태질소 함량과 글루탐산 등 유리아미노산 함량이 높아 관능평가에서 우수한 기호도가 나타난 연구결과가 있다(박신영 외, 2015, 한국식품저장유통학회, 22: 636-643). Astragalus membranaceus has a sweet taste, and there are research results showing excellent acceptability in sensory evaluation due to the high amino acid nitrogen content and free amino acid content such as glutamic acid in soy sauce with the addition of astragalus (Park Shin-young et al., 2015, Korea Food Storage Distribution) Society, 22: 636-643).
또한, 표고버섯(Lentinus edodes)은 강력한 감칠맛 성분인 구아닐산을 함유하고 있으며, 글루탐산 등 유리아미노산을 다른 버섯보다 많이 함유하고 있어 다양한 식품에 이용되고 있다.In addition, shiitake mushrooms ( Lentinus edodes ) contain guanylic acid, which is a strong umami component, and contain more free amino acids such as glutamic acid than other mushrooms, so they are used in various foods.
이에 본 발명자들은 관능적 품질에 영향을 주는 아미노태질소의 양, 유리아미노산의 양, 아미노산의 유리율, 글루탐산의 양 및 글루탐산의 유리율을 증가시킴으로써 짠맛의 감소와 구수한 맛의 핵심인 감칠맛을 증가시키는 효능을 갖는 황기와 표고버섯 혼합발효물을 유효성분으로 사용함으로써 풍미가 개선된 된장, 쌈장, 고추장 등의 식품조성물을 개발하여 본 발명에 이르게 되었다.Accordingly, the present inventors increased the amount of amino nitrogen, the amount of free amino acid, the release rate of amino acids, the amount of glutamic acid, and the release rate of glutamic acid, which affect sensory quality, thereby reducing salty taste and increasing umami, which is the core of savory taste. By using the fermented mixture of Astragalus and shiitake mushrooms having efficacy as an active ingredient, food compositions such as soybean paste, ssamjang, and red pepper paste with improved flavor were developed and led to the present invention.
본 발명은 아미노태질소의 양, 유리아미노산의 양, 아미노산의 유리율, 글루탐산의 양 및 글루탐산의 유리율을 증가시킴으로써 짠맛 감소와 구수한 맛의 핵심인 감칠맛을 증가시키는 효능을 갖는 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 풍미개선을 위한 식품조성물을 제공하는 것을 목적으로 한다.The present invention increases the amount of amino nitrogen, the amount of free amino acid, the release rate of amino acids, the amount of glutamic acid and the release rate of glutamic acid, thereby reducing the salty taste and increasing the savory taste, which is the core of the savory taste Astragalus and shiitake mushroom mixture An object of the present invention is to provide a food composition for flavor improvement containing fermented product as an active ingredient.
상기한 목적을 달성하기 위하여, 관능적 품질에 영향을 주는 아미노태질소의 양, 유리아미노산의 양, 아미노산의 유리율, 글루탐산의 양 및 글루탐산의 유리율을 증가시킴으로써 짠맛 감소와 구수한 맛의 핵심인 감칠맛을 증가시키는 효능을 갖는 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 풍미개선을 위한 된장, 쌈장, 고추장 등의 식품조성물을 제공하는 것을 특징으로 한다.In order to achieve the above object, by increasing the amount of amino nitrogen, the amount of free amino acid, the release rate of amino acids, the amount of glutamic acid and the release rate of glutamic acid, which affect the sensory quality, salty taste reduction and umami, which is the key to savory taste It is characterized by providing a food composition such as soybean paste, ssamjang, and gochujang for flavor improvement containing as an active ingredient a mixed fermented product of astragalus and shiitake mushroom having an effect of increasing.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명의 황기와 표고버섯 혼합발효물은 황기와 표고버섯을 간장에 침지하여 15일 이상 발효한 다음, 상기 황기분말과 건표고버섯이 함유된 간장을 면포로 감싸고 누름돌을 사용하여 하루 동안 상기 황기와 표고버섯을 간장으로부터 분리한 후, 상기 분리된 황기와 표고버섯을 핸드블렌더(필립스)로 3분간 분쇄 및 혼합하는 동안, 1분마다 뭉쳐있는 부분을 풀어주어 균일하게 되도록 하여 제조한다.The mixed fermented product of Astragalus and shiitake mushrooms of the present invention is fermented by immersing Astragalus and shiitake mushrooms in soy sauce for 15 days or more, then wrapping the soy sauce containing the Astragalus powder and dried shiitake mushrooms with a cotton cloth and using a pressing stone for a day. After separating the and shiitake mushrooms from the soy sauce, while the separated astragalus and shiitake mushrooms are ground and mixed with a hand blender (Philips) for 3 minutes, the clumped parts are loosened every minute to make them uniform.
본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장은 상기 황기와 표고버섯 혼합발효물을 된장 사입물과 배합 후 살균하여 제조한다.Doenjang containing the fermented mixture of Astragalus and shiitake mushrooms of the present invention as an active ingredient is prepared by mixing the fermented mixture of Astragalus and shiitake mushrooms with soybean paste and then sterilizing.
본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 쌈장은 상기 황기와 표고버섯 혼합발효물을 쌈장 사입물과 배합 후 살균하여 제조한다.Ssamjang containing the fermented mixture of Astragalus and shiitake mushrooms of the present invention as an active ingredient is prepared by mixing the fermented mixture of Astragalus and shiitake mushrooms with ssamjang and then sterilizing.
본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 고추장은 상기 황기와 표고버섯 혼합발효물을 고추장 사입물과 배합 후 살균하여 제조한다.Red pepper paste containing the fermented mixture of Astragalus and shiitake mushrooms of the present invention as an active ingredient is prepared by combining the fermented mixture of Astragalus and shiitake mushrooms with red pepper paste and then sterilizing.
본 발명은 관능적 품질에 영향을 주는 아미노태질소의 양, 유리아미노산의 양, 아미노산의 유리율, 글루탐산의 양 및 글루탐산의 유리율이 증가된 황기와 표고버섯 혼합발효물을 유효성분으로 사용함으로써 된장, 쌈장, 고추장 등의 식품조성물의 짠맛 감소와 구수한 맛의 핵심인 감칠맛을 증가시키는 효능을 갖는다.The present invention uses a mixed fermented product of astragalus and shiitake mushrooms with increased amounts of amino nitrogen, free amino acid, amino acid release rate, glutamic acid content and glutamic acid release rate, which affect sensory quality, as an active ingredient, soybean paste It has the effect of reducing the salty taste of food compositions such as , ssamjang and gochujang and increasing the savory taste, which is the core of the savory taste.
도 1은 전자혀를 이용하여 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장의 전자관능을 측정한 그래프이다.1 is a graph measuring the electronic function of soybean paste containing the mixed fermented product of Astragalus and shiitake mushrooms of the present invention using an electronic tongue as an active ingredient.
이하, 실시예를 통하여 본 발명을 보다 상세하게 설명한다. 그러나, 다음의 실시예는 본 발명의 범위를 한정하는 것은 아니며, 본 발명의 기술적 사상의 범위 내에서 통상의 지식을 가진 사람에 의한 통상적인 변화가 가능하다.Hereinafter, the present invention will be described in more detail through examples. However, the following examples do not limit the scope of the present invention, and ordinary changes by a person skilled in the art are possible within the scope of the technical spirit of the present invention.
<실시예 1><Example 1>
황기와 표고버섯 혼합발효물 제조Manufacture of mixed fermented product of Astragalus and shiitake mushrooms
국산 황기분말(강원도 홍천군) 1kg과 국산 건표고버섯(충남 서산시) 0.5kg을 양조간장(샘표식품, 양조간장 501) 10kg에 침지하여 15일 이상 발효하였다. 그런 다음 상기 황기분말과 건표고버섯이 함유된 간장을 면포로 감싸고 누름돌을 사용하여 하루 동안 상기 황기분말과 건표고버섯을 간장으로부터 분리하였다. 그런 다음 상기 분리된 황기분말과 건표고버섯을 핸드블렌더(필립스)로 3분 동안 분쇄 및 혼합하였고, 1분 마다 뭉쳐있는 부분을 풀어주어 균일하게 되도록 하여 본 발명의 황기와 표고버섯 혼합발효물을 제조하였다.1 kg of domestic astragalus powder (Hongcheon-gun, Gangwon-do) and 0.5 kg of domestic dried shiitake mushrooms (Seosan-si, Chungcheongnam-do) were immersed in 10 kg of brewed soy sauce (Sempio Foods, brewed soy sauce 501) and fermented for more than 15 days. Then, the soy sauce containing the astragalus powder and dried shiitake mushrooms was wrapped with a cotton cloth and the astragalus powder and dried shiitake mushrooms were separated from the soy sauce for one day using a pressing stone. Then, the separated Astragalus powder and dried shiitake mushrooms were pulverized and mixed with a hand blender (Philips) for 3 minutes, and the clumped parts were loosened every minute to make them uniform, so that the mixed fermented product of Astragalus and shiitake mushrooms of the present invention manufactured.
<실시예 2><Example 2>
황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장의 제조Preparation of Doenjang Containing Fermented Mixture of Astragalus and Shiitake Mushrooms as Active Ingredients
2-1. 된장사입물의 제조 2-1. Manufacturing of soybean paste products
본 발명의 된장 사입물은 대두 71.98중량%에 증자 소맥분 8.31중량%, 소맥분 곡자(Koji) 8.31중량% 및 정제염 11.4중량%를 혼합하여 제조하였다.The doenjang insert of the present invention was prepared by mixing 71.98% by weight of soybeans with 8.31% by weight of steamed wheat flour, 8.31% by weight of wheat flour grains (Koji) and 11.4% by weight of refined salt.
즉, 대두는 수세 후 약 1.6배의 정제수를 가하여 22±5~24±5hr 침지하고, 1.9kgf/㎠의 압력으로 4~6분간 증자 후 30±3℃까지 냉각하여 사용하였고, 증자 소맥분은 소맥분을 1.5±0.5kgf/㎠의 압력에서 증자 후 파쇄하여 냉각한 것을 사용하였으며, 소맥분 곡자(Koji)는 상기 증자 소맥분에 종균을 접종하여 35±5℃에서 제국한 것을 사용하였다. 상기 방법으로 제조된 대두 71.98중량%, 증자 소맥분 8.31중량% 및 소맥분 곡자 8.31중량%를 혼합하여 공기가 통하지 않도록 눌러 담고, 그 상부에 정제염 11.4중량%를 고루 뿌린 후 비닐로 덮어 30일간 숙성하였다. 이렇게 숙성이 완료된 것을 본 발명의 된장 사입물로 사용하였다.That is, after washing the soybeans, about 1.6 times more purified water was added, immersed for 22 ± 5 to 24 ± 5 hr, steamed for 4 to 6 minutes at a pressure of 1.9 kgf / ㎠, and then cooled to 30 ± 3 ° C. After steaming at a pressure of 1.5 ± 0.5 kgf / cm 2, crushing and cooling were used, and wheat flour grains (Koji) were used to inoculate the steamed wheat flour with a spawner and make it at 35 ± 5 ° C. 71.98% by weight of soybeans, 8.31% by weight of steamed wheat flour, and 8.31% by weight of wheat flour grains prepared by the above method were mixed and pressed so as not to pass through the air, and 11.4% by weight of refined salt was evenly sprinkled on top of it. Covered with vinyl and aged for 30 days. The matured soybean paste of the present invention was used as an additive.
2-2. 황기와 표고버섯 혼합발효물과 된장사입물의 배합 및 살균 2-2. Mixing and sterilization of mixed fermented product of Astragalus and shiitake mushrooms and soybean paste
상기 실시예 2-1의 된장사입물 87.3중량%, 상기 실시예 1의 황기와 표고버섯 혼합발효물 9중량%, L-글루탐산나트륨 0.5중량%, 볶음대두분 0.7중량% 및 주정 2.5중량%를 제과 반죽용 혼합기(키친에이드)를 사용하여 3분 동안 혼합하였고, 1분마다 뭉쳐있는 부분을 풀어주어 균일하게 혼합되도록 하였다. 그런 다음, 상기 혼합된 된장을 유리용기에 충진한 후, 항온수조를 사용하여 65℃에서 15분간 중탕을 실시하였다. 이때, 중탕하는 동안 균질기를 사용하여 100RPM의 속도로 된장을 혼합하여 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장을 제조하였다.87.3% by weight of the soybean paste prepared in Example 2-1, 9% by weight of the mixed fermented product of Astragalus and shiitake mushrooms in Example 1, 0.5% by weight of sodium L-glutamate, 0.7% by weight of roasted soybean powder and 2.5% by weight of alcohol It was mixed for 3 minutes using a confectionery dough mixer (Kitchen Aid), and the clumped parts were loosened every minute to ensure uniform mixing. Then, after filling the mixed doenjang in a glass container, water bath was performed at 65° C. for 15 minutes using a constant temperature water bath. At this time, doenjang was mixed at a speed of 100 RPM using a homogenizer during bathing to prepare doenjang containing the mixed fermented product of Astragalus root and shiitake mushroom of the present invention as an active ingredient.
<실시예 3><Example 3>
황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 고추장의 제조Preparation of red pepper paste containing astragalus and shiitake mixed fermented product as an active ingredient
3-1. 고추장사입물의 제조 3-1. Manufacture of red pepper paste paste
본 발명의 고추장사입물은 밀쌀 53.36중량%와 소맥분 곡자(koji) 46.64중량%를 혼합하여 제조하였다.The gochujang paste of the present invention was prepared by mixing 53.36% by weight of wheat rice and 46.64% by weight of wheat flour grains (koji).
즉, 밀쌀은 수세 후 밀쌀의 4배 분량 물에 1~2hr 침지하여 1.8~2.0kg/㎠의 압력으로 30~50분간 증자 후 냉각하여 사용하였고, 소맥분 곡자(koji)는 소맥분을 1.0~2.0kg/㎠의 압력으로 증자 후 파쇄하여 냉각하고, 종균을 접종하여 30~37℃에서 36~40hr 입국한 후, 제국이 완료된 것을 사용하였다. 상기 방법으로 제조된 밀쌀 53.36중량%와 소맥분 곡자(koji) 46.64중량%를 혼합하여 공기가 통하지 않도록 눌러 담은 후 비닐로 덮어 15일간 숙성하였다. 이렇게 숙성이 완료된 것을 본 발명의 고추장사입물로 사용하였다.That is, wheat rice was washed with water, immersed in water four times the amount of wheat rice for 1 to 2 hours, steamed for 30 to 50 minutes at a pressure of 1.8 to 2.0 kg/cm2, and then cooled and used. / cm 2 pressure, then crushing and cooling, inoculating the spawn, entering the country at 30 ~ 37 ℃ for 36 ~ 40 hr, and then using the finished empire. 53.36% by weight of wheat flour prepared by the above method and 46.64% by weight of wheat flour grain (koji) were mixed, pressed so as not to pass through the air, and then covered with plastic and aged for 15 days. This aging was completed was used as a red pepper paste paste of the present invention.
3-2. 황기와 표고버섯 혼합발효물과 고추장사입물의 배합 및 살균 3-2. Mixture and sterilization of mixed fermented product of Astragalus and shiitake mushrooms and red pepper paste
상기 실시예 3-1의 고추장사입물 40.64중량%, 상기 실시예 1의 황기와 표고버섯 혼합발효물 4중량%, 정제염 5.01중량%, 맥아엿 28중량%, 고춧가루 6중량%, 혼합조미료 13.95중량%(혼합조미료는 고춧가루 38중량%, 마늘 7중량%, 양파 4중량%, 정제염 15중량% 및 정제수 36중량%를 혼합하여 공지의 방법으로 제조된 것임), 혼합미분 0.7중량%(혼합미분은 밀쌀 55중량%, 쌀 35중량%, 소맥분 10중량%를 혼합하여 공지의 방법으로 제조된 것임) 및 주정 1.7중량%를 제과 반죽용 혼합기(키친에이드)를 사용하여 3분 동안 혼합하였고, 1분마다 뭉쳐있는 부분을 풀어주어 균일하게 혼합되도록 하였다. 그런 다음, 상기 혼합된 고추장을 유리용기에 충진한 후, 항온수조를 사용하여 65℃에서 15분간 중탕을 실시하였다. 이때 중탕하는 동안 균질기를 사용하여 100RPM의 속도로 고추장을 혼합하여 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 고추장을 제조하였다.40.64% by weight of the red pepper paste paste of Example 3-1, 4% by weight of the mixed fermented product of Astragalus and shiitake mushrooms of Example 1, 5.01% by weight of refined salt, 28% by weight of malt syrup, 6% by weight of red pepper powder, 13.95% by weight of mixed seasoning % (mixed seasoning is prepared by a known method by mixing red pepper powder 38% by weight, garlic 7% by weight, onion 4% by weight, refined salt 15% by weight and purified water 36% by weight), mixed flour 0.7% by weight (mixed fine powder is It is prepared by a known method by mixing 55% by weight of wheat rice, 35% by weight of rice, and 10% by weight of wheat flour) and 1.7% by weight of alcohol were mixed for 3 minutes using a confectionery dough mixer (Kitchen Aid), and 1 minute The clumped parts were loosened each time to ensure uniform mixing. Then, after filling the mixed gochujang in a glass container, water bath was performed at 65 ° C. for 15 minutes using a constant temperature water bath. At this time, red pepper paste was prepared by mixing red pepper paste at a speed of 100 RPM using a homogenizer during hot water bathing to prepare red pepper paste containing the mixed fermented product of Astragalus and shiitake mushrooms of the present invention as an active ingredient.
<실시예 4><Example 4>
황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 쌈장의 제조Preparation of ssamjang containing astragalus and shiitake mixed fermented product as an active ingredient
4-1. 쌈장사입물의 제조 4-1. Manufacture of ssamjang inserts
본 발명의 쌈장사입물은 상기 실시예 2-1의 된장사입물 77.5중량%와 상기 실시예 3-1의 고추장사입물 22.5중량%를 혼합한 것을 쌈장사입물로 사용하였다.The ssamjang filling of the present invention was a mixture of 77.5% by weight of the soybean paste filling of Example 2-1 and 22.5% by weight of the red pepper paste filling of Example 3-1.
4-2. 황기와 표고버섯 혼합발효물과 쌈장사입물의 배합 및 살균 4-2. Mixing and sterilization of mixed fermented product of Astragalus and shiitake mushrooms and ssamjang sauce
상기 실시예 4-1의 쌈장사입물 54중량%, 상기 실시예 1의 황기와 표고버섯 혼합발효물 6중량%, 정제수 7.3중량%, 물엿 13.0중량%, 백설탕 4.5중량%, 대두분 5.0중량%, 혼합조미료 2.5중량%(혼합조미료는 고춧가루 38중량%, 마늘 7중량%, 양파 4중량%, 정제염 15중량% 및 정제수 36중량%를 혼합하여 공지의 방법으로 제조된 것임), 볶음참깨 1.5중량%, 생마늘 1.0중량%, 마늘 농축액 0.7중량%, 양파 농축액 0.5중량%, 대파 농축액 0.3중량%, 생강 농축액 0.2중량%, 정제염 0.2중량%, 참기름 0.2중량%, 효모분말 0.3중량%, 오레오레진캡시켐 0.1중량% 및 주정 2.7중량%를 제과 반죽용 혼합기(키친에이드)를 사용하여 3분 동안 혼합하였고, 1분마다 뭉쳐있는 부분을 풀어주어 균일하게 혼합되도록 하였다. 그런 다음, 상기 혼합된 쌈장을 유리용기에 충진한 후, 항온수조를 사용하여 65℃에서 15분간 중탕을 실시하였다. 이때 중탕하는 동안 균질기를 사용하여 100RPM의 속도로 쌈장을 혼합하여 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 쌈장을 제조하였다.54% by weight of the ssamjang paste of Example 4-1, 6% by weight of the mixed fermented product of Astragalus and shiitake mushroom of Example 1, 7.3% by weight of purified water, 13.0% by weight of starch syrup, 4.5% by weight of white sugar, 5.0% by weight of soybean powder , 2.5% by weight of mixed seasoning (mixed seasoning is prepared by a known method by mixing 38% by weight of red pepper powder, 7% by weight of garlic, 4% by weight of onion, 15% by weight of refined salt and 36% by weight of purified water), 1.5% by weight of fried sesame seeds %, raw garlic 1.0% by weight, garlic concentrate 0.7% by weight, onion concentrate 0.5% by weight, green onion concentrate 0.3% by weight, ginger concentrate 0.2% by weight, refined salt 0.2% by weight, sesame oil 0.2% by weight, yeast powder 0.3% by weight, oreo resin cap Seachem 0.1% by weight and alcohol 2.7% by weight were mixed for 3 minutes using a confectionery dough mixer (Kitchen Aid), and the clumped parts were loosened every minute to ensure uniform mixing. Then, after filling the mixed ssamjang in a glass container, water bath was performed at 65 ° C. for 15 minutes using a constant temperature water bath. At this time, ssamjang was mixed at a speed of 100 RPM using a homogenizer during bathing to prepare ssamjang containing the mixed fermented product of Astragalus and shiitake mushroom of the present invention as an active ingredient.
<비교예 1><Comparative Example 1>
일반된장의 제조Manufacturing of general soybean paste
상기 실시예 2-1의 된장사입물 96.3중량%, L-글루탐산나트륨 0.5중량%, 볶음대두분 0.7중량% 및 주정 2.5중량%를 제과 반죽용 혼합기(키친에이드)를 사용하여 3분 동안 혼합하였고, 1분마다 뭉쳐있는 부분을 풀어주어 균일하게 혼합되도록 하였다. 그런 다음, 상기 혼합된 된장을 유리용기에 충진한 후, 항온수조를 사용하여 65℃에서 15분간 중탕을 실시하였다. 이때 중탕하는 동안 균질기를 사용하여 100RPM의 속도로 된장을 혼합하여 일반 된장을 제조하였다.96.3% by weight of the soybean paste preparation of Example 2-1, 0.5% by weight of sodium L-glutamate, 0.7% by weight of roasted soybean flour, and 2.5% by weight of alcohol were mixed for 3 minutes using a pastry mixer (Kitchen Aid) , the clumped parts were loosened every minute to ensure uniform mixing. Then, after filling the mixed doenjang in a glass container, water bath was performed at 65° C. for 15 minutes using a constant temperature water bath. At this time, general doenjang was prepared by mixing the doenjang at a speed of 100 RPM using a homogenizer during hot water bath.
<비교예 2><Comparative Example 2>
일반고추장의 제조Manufacture of regular red pepper paste
상기 실시예 3-1의 고추장사입물 44.64중량%, 정제염 5.01중량%, 맥아엿 28중량%, 고춧가루 6중량%, 혼합조미료 13.95중량%(혼합조미료는 고춧가루 38중량%, 마늘 7중량%, 양파 4중량%, 정제염 15중량% 및 정제수 36중량%를 혼합하여 공지의 방법으로 제조된 것임), 혼합미분 0.7중량%(혼합미분은 밀쌀 55중량%, 쌀 35중량%, 소맥분 10중량%를 혼합하여 공지의 방법으로 제조된 것임) 및 주정 1.7중량%를 혼합하고 제과 반죽용 혼합기(키친에이드)를 사용하여 3분 동안 혼합하였고, 1분마다 뭉쳐있는 부분을 풀어주어 균일하게 혼합되도록 하였다. 그런 다음, 상기 혼합된 고추장을 유리용기에 충진한 후, 항온수조를 사용하여 65℃에서 15분간 중탕을 실시하였다. 이때 중탕하는 동안 균질기를 사용하여 100RPM의 속도로 고추장을 혼합하여 일반 고추장을 제조하였다.44.64% by weight of the red pepper paste paste of Example 3-1, 5.01% by weight of refined salt, 28% by weight of malt syrup, 6% by weight of red pepper powder, 13.95% by weight of mixed seasoning (mixed seasoning is 38% by weight of red pepper powder, 7% by weight of garlic, onion It is prepared by a known method by mixing 4% by weight, 15% by weight of refined salt and 36% by weight of purified water), 0.7% by weight of mixed flour (the mixed fine powder is a mixture of 55% by weight of wheat rice, 35% by weight of rice, and 10% by weight of wheat flour) prepared by a known method) and 1.7% by weight of alcohol were mixed and mixed for 3 minutes using a mixer for confectionery dough (Kitchen Aid), and the clumped parts were loosened every minute to ensure uniform mixing. Then, after filling the mixed gochujang in a glass container, water bath was performed at 65 ° C. for 15 minutes using a constant temperature water bath. At this time, regular gochujang was prepared by mixing the gochujang at a speed of 100 RPM using a homogenizer while bathing.
<비교예 3><Comparative Example 3>
일반 쌈장의 제조Manufacturing of general ssamjang
상기 실시예 4-1의 쌈장사입물 60중량%, 정제수 7.3중량%, 물엿 13.0중량%, 백설탕 4.5중량%, 대두분 5.0중량%, 혼합조미료 2.5중량%(혼합조미료는 고춧가루 38중량%, 마늘 7중량%, 양파 4중량%, 정제염 15중량% 및 정제수 36중량%를 혼합하여 공지의 방법으로 제조된 것임), 볶음참깨 1.5중량%, 생마늘 1.0중량%, 마늘 농축액 0.7중량%, 양파 농축액 0.5중량%, 대파 농축액 0.3중량%, 생강 농축액 0.2중량%, 정제염 0.2중량%, 참기름 0.2중량%, 효모분말 0.3중량%, 오레오레진캡시켐 0.1중량% 및 주정 2.7중량%를 제과 반죽용 혼합기(키친에이드)를 사용하여 3분 동안 혼합하였고, 1분마다 뭉쳐있는 부분을 풀어주어 균일하게 혼합되도록 하였다. 그런 다음, 상기 혼합된 쌈장을 유리용기에 충진한 후, 항온수조를 사용하여 65℃에서 15분간 중탕을 실시하였다. 이때 중탕하는 동안 균질기를 사용하여 100RPM의 속도로 쌈장을 혼합하여 일반 쌈장을 제조하였다.60% by weight of the ssamjang stock of Example 4-1, 7.3% by weight of purified water, 13.0% by weight of starch syrup, 4.5% by weight of white sugar, 5.0% by weight of soybean flour, 2.5% by weight of mixed seasoning (mixed seasoning is 38% by weight of red pepper powder, garlic 7% by weight onion, 4% by weight of onion, 15% by weight of refined salt and 36% by weight of purified water) prepared by a known method), 1.5% by weight of fried sesame, 1.0% by weight of raw garlic, 0.7% by weight of garlic concentrate, 0.5% onion concentrate % by weight, 0.3% by weight of green onion concentrate, 0.2% by weight of ginger concentrate, 0.2% by weight of refined salt, 0.2% by weight of sesame oil, 0.3% by weight of yeast powder, 0.1% by weight of Oreo Resin Capsichem, and 2.7% by weight of alcohol in a mixer for confectionery dough ( KitchenAid) was used to mix for 3 minutes, and the clumped parts were loosened every 1 minute to ensure uniform mixing. Then, after filling the mixed ssamjang in a glass container, water bath was performed at 65 ° C. for 15 minutes using a constant temperature water bath. At this time, general ssamjang was prepared by mixing the ssamjang at a speed of 100 RPM using a homogenizer while bathing.
<시험예 1><Test Example 1>
이화학 특성 측정Measurement of physical and chemical properties
상기 실시예 2의 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장과 상기 비교예 1의 일반된장의 이화학 특성을 다음과 같이 측정하였다.Physicochemical properties of doenjang containing the mixed fermented product of Astragalus and shiitake mushroom of the present invention of Example 2 as an active ingredient and general doenjang of Comparative Example 1 were measured as follows.
pH는 시료 5g을 10배 희석하여 충분히 교반한 후에 pH미터(Mettler Toledo S220, Switzerland)로 측정하였다.pH was measured with a pH meter (Mettler Toledo S220, Switzerland) after diluting 5 g of the
수분 함량은 시료 2g을 적외선수분측정기(Ohous MB25, Korea)를 이용하여 측정하였다.Moisture content was measured using an infrared moisture meter (Ohous MB25, Korea) for 2 g of the sample.
당도는 시료 5g을 10배 희석하여 충분히 교반한 후에 디지털 굴절계(Atago RX-5000a, Japan)를 이용하여 측정한 후, 희석배수를 곱하였다. Sugar content was measured using a digital refractometer (Atago RX-5000a, Japan) after diluting 5 g of the
염도는 시료 5g을 10배 희석하여 충분히 교반한 후에 디지털 염도계(CAS salt-free 1000, Korea)를 이용하여 측정한 후, 희석배수를 곱하였다.Salinity was measured using a digital salinity meter (CAS salt-free 1000, Korea) after diluting 5 g of the
아미노태질소는 Formol 적정법에 준하여 측정하였다. 즉, 시료 5g을 취하여 증류수 250mL를 가하고, 여과하여 여액을 50mL 취하였다. 지시약으로 페놀프탈레인을 2~3 방울 가하고 0.1N NaOH 용액으로 미홍색이 될 때까지 적정하였다. 여기에 포르말린 용액을 30mL 가하고 다시 0.1N NaOH 용액으로 미홍색이 될 때까지 적정하였다.Amino nitrogen was measured according to the Formol titration method. That is, 5 g of the sample was taken, 250 mL of distilled water was added, filtered, and 50 mL of the filtrate was obtained. 2-3 drops of phenolphthalein was added as an indicator and titrated with 0.1N NaOH solution until it turned pink. 30 mL of a formalin solution was added thereto, and titration was performed again with a 0.1N NaOH solution until it turned pink.
그 결과를 하기의 표 1에 나타내었다.The results are shown in Table 1 below.
표 1에서 확인할 수 있는 바와 같이, 실시예 2의 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장과 비교예 1의 일반된장 사이의 Brix, 염도, pH에서는 크게 차이가 없었으나, 실시예 2의 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장에서 된장 풍미의 측정지표인 아미노태질소 함량이 비교예 1의 일반 된장에서의 아미노태질소 함량과 비교하여 증가하는 것으로 나타나 풍미가 개선되는 것을 알 수 있었다.As can be seen in Table 1, there was no significant difference in Brix, salinity, and pH between the doenjang containing the mixed fermented product of Astragalus and shiitake mushroom of the present invention of Example 2 as an active ingredient and the general doenjang of Comparative Example 1, , In the doenjang containing the mixed fermented product of Astragalus and shiitake mushroom of the present invention as an active ingredient in Example 2, the content of amino nitrogen, which is a measure of the flavor of miso, is increased compared to the content of amino nitrogen in the general doenjang of Comparative Example 1 It was found that the flavor was improved.
<시험예 2><Test Example 2>
아미노산 유리율 측정Measurement of amino acid release rate
2-1. 총 아미노산 측정 2-1. Total Amino Acid Measurement
상기 실시예 2의 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장 시료와 상기 비교예 1의 일반 된장 시료를 동결건조한 후, 분말로하여 각각 균질화한 시료 10mg을 0.05% 2-머캅토에탄올을 함유한 6N 염산 10mL 가하여 동결한 후, 탈기장치를 통해 탈기하였다. 그런 다음 봉관하여 정온가열로에서 110℃에서 24시간 동안 가수분해한 다음, 봉관을 절단하여 즉시 감압 후 40℃에서 농축 건조하여 염산을 제거하였다. 상기 잔사물을 0.2N 구연산나트륨 완충액(pH 2.2)으로 용해하여 만들어진 것을 시험용액으로 하였다. 아미노산 자동분석기에 시험용액과 표준용액(아미노산)을 동일한 조건에서 분석하여 머무름 시간이 일치하는 성분을 비교 동정한 뒤, 표준용액으로부터 얻어진 피크면적으로 시험용액 안의 아미노산 함량을 아미노산 g/시료 100g 으로 계산하였다. After freeze-drying the soybean paste sample containing the mixed fermented product of Astragalus and shiitake mushroom of the present invention of Example 2 as an active ingredient and the general soybean paste sample of Comparative Example 1, 10 mg of each homogenized sample in powder was 0.05% 2- After freezing by adding 10 mL of 6N hydrochloric acid containing mercaptoethanol, it was degassed through a deaerator. Then, the sealed tube was hydrolyzed at 110 ° C. for 24 hours in a constant temperature heating furnace, and then the sealed tube was cut and immediately concentrated and dried at 40 ° C. after reducing pressure to remove hydrochloric acid. A test solution prepared by dissolving the residue in 0.2N sodium citrate buffer (pH 2.2) was used. After analyzing the test solution and the standard solution (amino acid) under the same conditions with an automatic amino acid analyzer, components with the same retention time are compared and identified, and the amino acid content in the test solution is calculated as amino acid g/100g of sample with the peak area obtained from the standard solution. did
그 결과를 하기의 표 2에 나타내었다.The results are shown in Table 2 below.
표 2에서 확인할 수 있는 바와 같이, 비교예 1의 총 아미노산 함량은 아미노산 12.014g/시료 100g인 반면, 실시예 2의 총 아미노산 함량은 아미노산 10.759g/시료 100g으로써 총 아미노산 함량에 있어서 실시예 2가 비교예 1보다 아미노산 1.255g/시료 100g 적은 것을 알 수 있었다.As can be seen in Table 2, the total amino acid content of Comparative Example 1 was 12.014 g of amino acids/100 g of sample, whereas the total amino acid content of Example 2 was 10.759 g of amino acids/100 g of sample, and Example 2 in terms of total amino acid content It was found that 1.255 g of amino acid/100 g of sample was less than that of Comparative Example 1.
2-2. 유리아미노산 측정 2-2. free amino acid determination
상기 실시예 2의 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장 시료와 상기 비교예 1의 일반 된장 시료의 각각 1g에 10배의 물을 가해 비등 수욕 중에 가열하여 응고시킨 다음 여과하여 물층을 취하였다. 이를 감압농축하여 건조시킨 잔사물을 0.2N 구연산나트륨 완충액(pH 2.2)으로 용해하여 만들어진 것을 시험용액으로 하였다. 아미노산 자동분석기에 시험용액과 표준용액(아미노산)을 동일한 조건에서 분석하여 머무름 시간이 일치하는 성분을 비교 동정한 뒤, 표준용액으로부터 얻어진 피크면적으로 시험용액안의 아미노산 함량을 아미노산 g/시료 100g으로 계산하였다. 10 times as much water was added to each of the soybean paste sample containing the mixed fermented product of Astragalus and shiitake mushroom of the present invention of Example 2 as an active ingredient and the general soybean paste sample of Comparative Example 1, respectively, and solidified by heating in a boiling water bath. The water layer was obtained by filtration. A test solution was prepared by dissolving the dried residue obtained by concentrating under reduced pressure in 0.2N sodium citrate buffer (pH 2.2). After analyzing the test solution and the standard solution (amino acid) under the same conditions with an automatic amino acid analyzer, components with the same retention time are compared and identified, and the amino acid content in the test solution is calculated as amino acid g/100g of sample by the peak area obtained from the standard solution. did
그 결과를 하기의 표 3에 나타내었다.The results are shown in Table 3 below.
표 3에서 확인할 수 있는 바와 같이, 비교예 1의 총 유리아미노산 함량은 아미노산 2.839g/시료 100g인 반면, 실시예 2의 총 유리아미노산 함량은 아미노산 2.962g/시료 100g으로써 총 유리아미노산 함량에 있어서 실시예 2가 비교예 1보다 4.33% 높은 것을 알 수 있었다.As can be seen in Table 3, the total free amino acid content of Comparative Example 1 was 2.839 g of amino acid/100 g of sample, whereas the total free amino acid content of Example 2 was 2.962 g of amino acid/100 g of sample, which is It was found that Example 2 was 4.33% higher than Comparative Example 1.
또한, 비교예 1의 총 유리아미노산 중 글루탐산의 함량은 아미노산 0.668g/시료 100g인 반면, 실시예 2의 총 유리아미노산 중 글루탐산의 함량은 아미노산 0.723g/시료 100g으로써 총 유리아미노산 중 글루탐산의 함량에 있어서 실시예 2가 비교예 1보다 8.23% 높은 것을 알 수 있었다.In addition, the content of glutamic acid among the total free amino acids of Comparative Example 1 was 0.668 g of amino acids/100 g of sample, whereas the content of glutamic acid among the total free amino acids of Example 2 was 0.723 g of amino acids/100 g of sample, which was proportional to the content of glutamic acid among total free amino acids. It was found that Example 2 was 8.23% higher than Comparative Example 1.
2-3. 아미노산의 유리율 2-3. Amino Acid Free Rate
상기 시험예 2-1의 총 아미노산 측정값과 상기 시험예 2-2의 유리아미노산 측정값을 토대로 상기 실시예 2의 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장 시료와 상기 비교예 1의 일반 된장 시료의 아미노산의 유리율(%)에 대해 총 아미노산 대비 유리아미노산의 비율로 환산하여 상기 각 시료의 아미노산의 유리율을 하기의 표 4에 나타내었다. Based on the measured values of total amino acids in Test Example 2-1 and the measured values of free amino acids in Test Example 2-2, a soybean paste sample containing the mixed fermented product of Astragalus root and shiitake mushroom of the present invention in Example 2 as an active ingredient and the above The amino acid release rate (%) of the general soybean paste sample of Comparative Example 1 was converted into the ratio of free amino acid to total amino acids, and the amino acid release rate of each sample is shown in Table 4 below.
표 4에서 확인할 수 있는 바와 같이, 비교예 1의 평균 아미노산 유리율은 23.60%인 반면, 실시예 2의 평균 아미노산 유리율은 27.84%로써 아미노산의 유리율에 있어서 실시예 2가 비교예 1보다 4.24% 높은 것을 알 수 있었다.As can be seen in Table 4, the average amino acid release rate of Comparative Example 1 is 23.60%, whereas the average amino acid release rate of Example 2 is 27.84%, and Example 2 is 4.24% higher than Comparative Example 1 in the release rate of amino acids. % was found to be high.
또한, 비교예 1의 글루탐산의 유리율은 22.22%인 반면, 실시예 2의 글루탐산의 유리율은 25.41%로써 글루탐산의 유리율에 있어서 실시예 2가 비교예 1보다 3.19% 높은 것을 알 수 있었다.In addition, the glutamic acid release rate of Comparative Example 1 was 22.22%, whereas the glutamic acid release rate of Example 2 was 25.41%, indicating that Example 2 was 3.19% higher than Comparative Example 1.
이상에서와 같이, 총 아미노산의 함량에 있어서는 실시예 2가 비교예 1보다 적음에도 불구하고, 총 유리아미노산의 함량 및 아미노산의 평균 유리율에 있어서는 실시예 2가 비교예 1보다 증가하였고, 특히, 유리아미노산 중 글루탐산의 양과 글루탐산의 유리율은 실시예 2가 비교예 1보다 증가하였음을 알 수 있었다. As described above, although Example 2 was smaller than Comparative Example 1 in terms of total amino acid content, Example 2 was higher than Comparative Example 1 in terms of total free amino acid content and average amino acid release rate. In particular, It was found that the amount of glutamic acid among free amino acids and the release rate of glutamic acid in Example 2 were increased compared to Comparative Example 1.
따라서, 실시예 2의 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장이 비교예 1의 일반 된장 보다 풍미 및 관능이 향상되었음을 알 수 있었다.Therefore, it was found that the doenjang containing the mixed fermented product of Astragalus and shiitake mushroom of the present invention of Example 2 as an active ingredient improved the flavor and sensory compared to the general doenjang of Comparative Example 1.
<시험예 3> <Test Example 3>
전자혀를 이용한 전자 관능 측정Electronic sensory measurement using an electronic tongue
상기 실시예 2의 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장 시료와 상기 비교예 1의 일반 된장 시료를 각각 30g씩 취하여, 300mL의 끓는 물에 풀어 3분간 가열하여 된장국을 조리한 후, 적절히 희석하여 전자혀 분석에 사용하였다. 30 g each of the soybean paste sample containing the mixed fermented product of Astragalus and shiitake mushroom of the present invention of Example 2 and the general soybean paste sample of Comparative Example 1 were taken, dissolved in 300mL of boiling water, and heated for 3 minutes to make miso soup. After cooking, it was appropriately diluted and used for electronic tongue analysis.
각 시료의 맛은 7개의 전자화학 센서(SRS, GPS, STS, UMS, SPS, SWS, BRS)와 1개의 기준 전극(Ag/AgCl)이 부착된 전자혀(ASTREE, Alpha MOS, Toulouse, France)를 이용하여 분석하였다. 이때 SRS는 신맛, STS는 짠맛, UMS는 감칠맛, SWS는 단맛, BRS는 쓴맛을 감지하고, GPS와 SPS는 센서값을 보정하는 표준센서로 사용하였다. The taste of each sample was evaluated using an electronic tongue (ASTREE, Alpha MOS, Toulouse, France) equipped with seven electrochemical sensors (SRS, GPS, STS, UMS, SPS, SWS, BRS) and one reference electrode (Ag/AgCl). was analyzed using . At this time, SRS detects sour taste, STS detects salty taste, UMS detects savory taste, SWS detects sweet taste, BRS detects bitter taste, and GPS and SPS are used as standard sensors to calibrate sensor values.
상기 된장국 시료 25mL를 120초 동안 분석하였고, 시료 간 오염을 방지하기 위해 증류수로 세척하였으며, 3회 분석결과를 주성분 분석에 사용하였다.25 mL of the miso soup sample was analyzed for 120 seconds, washed with distilled water to prevent contamination between samples, and the analysis results three times were used for main component analysis.
그 결과를 도 1에 나타내었다.The results are shown in Figure 1.
도 1에서 확인할 수 있는 바와 같이, 실시예 2의 본 발명의 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 된장을 사용한 된장국이 비교예 1의 일반 된장을 사용한 된장국에 비해 짠맛을 소폭 감소시켰고, 그 외의 모든 맛에 있어서는 증가하여 풍미가 향상되었고, 특히, 구수한 맛의 핵심인 감칠맛이 증가하였음을 알 수 있었다.As can be seen in Figure 1, the miso soup using miso containing the mixed fermented product of Astragalus and shiitake mushroom of the present invention of Example 2 as an active ingredient compared to miso soup using common miso of Comparative Example 1 Slightly reduced saltiness , In all other tastes, it was found that the flavor was improved by increasing, and in particular, the umami, which is the core of the savory taste, increased.
Claims (3)
A food composition for flavor improvement containing astragalus and shiitake mixed fermented product as an active ingredient.
상기 황기와 표고버섯 혼합발효물은 황기와 표고버섯을 간장에 발효시킨 것을 특징으로 하는 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 풍미개선을 위한 식품조성물.
The method of claim 1,
The astragalus and shiitake mixed fermented product is a food composition for flavor improvement containing astragalus and shiitake mixed fermented product as an active ingredient, characterized in that fermented astragalus and shiitake mushrooms in soy sauce.
상기 식품조성물은 된장, 쌈장, 고추장 중 어느 하나인 것을 특징으로 하는 황기와 표고버섯 혼합발효물을 유효성분으로 함유하는 풍미개선을 위한 식품조성물.The method of claim 1,
The food composition is a food composition for flavor improvement containing astragalus and shiitake mixed fermented product as an active ingredient, characterized in that any one of soybean paste, ssamjang, and red pepper paste.
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KR20060040797A (en) | 2004-11-05 | 2006-05-10 | 학교법인 영남학원 | Method for preparing soybean paste and soy sauce with good flavor and reduced nasty smell |
KR20160126780A (en) | 2015-04-24 | 2016-11-02 | 고로쇠산삼농원 영농조합법인 | manufacturing method of soybean paste improving flavor |
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2021
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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KR20060040797A (en) | 2004-11-05 | 2006-05-10 | 학교법인 영남학원 | Method for preparing soybean paste and soy sauce with good flavor and reduced nasty smell |
KR20160126780A (en) | 2015-04-24 | 2016-11-02 | 고로쇠산삼농원 영농조합법인 | manufacturing method of soybean paste improving flavor |
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