KR20230071796A - Method for cultivation of soybean plant with increased isoflavone derivatives by using a new smart-metabolite chamber - Google Patents

Abstract

In the present invention, a smart-metabolite chamber that can be cultivated to increase the content of isoflavone derivatives in soybean plants and a method for cultivating soybean plants enriched with isoflavone derivatives using the same are disclosed.
According to the soybean plant cultivation method using the smart-metabolite chamber according to the present invention, soybean plants with a high content of isoflavone derivatives can be grown in a short period of time (20 to 40 days). In addition, the cultivation method of the present invention can stably produce soybean plants containing high isoflavone derivatives throughout the year. In addition, soybean leaves, soybean stalks, and soybean roots, extracts or fractions thereof, which are soybean plants containing high isoflavone derivatives according to the present invention, can be used as estrogen substitutes, for prevention and improvement of diseases caused by estrogen deficiency, for antioxidant, And it is useful as a material for pharmaceutical compositions for skin care, liver function foods and cosmetics.

Description

Method for cultivation of soybean plant with increased isoflavone derivatives by using a new smart-metabolite chamber}

The present invention relates to a method for cultivating soybean plants with enhanced isoflavone derivatives using a smart-metabolite chamber, and more particularly, to a smart-metabolite chamber (smart-metabolite chamber that can be cultivated to increase the content of isoflavone derivatives in soybean plants). -metabolite chamber) and a method for cultivating soybean plants enriched with isoflavone derivatives using the same.

Estrogen, a female hormone, begins to decrease after the age of 40 and ultimately causes menopause. When estrogen is reduced, aging begins in the bones, heart, skin, brain, etc., which are the action points of this hormone. In order to suppress this aging process, estrogen substitutes are desperately needed, and isoflavone derivatives having structural similarities have been reported as effective estrogen substitutes.

Isoflavone derivatives are typical flavonoids with excellent pharmacological efficacy, and a lot of capital is being invested in the development of functional plants containing them in high quantities worldwide. Isoflavones are non-steroidal estrogens, which are called phytoestrogens because their chemical and physiological properties are similar to those of estrogen, and are substances that act as estrogen or anti-estrogen by competitively binding to estrogen receptors (Hudson, 2001). They are similar in structure to the female hormone estrogen and show useful physiological activities that appear in estrogen. Ingestion of isoflavones is known to regulate female endocrine status and affect ovarian cycles (Kenneth, Setchell, & Cassidy, 1999). In particular, it has been reported to reduce the risk of osteoporosis, which is one of the menopausal symptoms that can occur due to lack of female hormones, and to lower plasma cholesterol levels (Obstetrics & Gynecology 2001. 389). It is also known to have an effect of lowering the risk of coronary arterial heart disease (CAHD) and excellent antioxidant activity.

Among isoflavone derivatives, non-glycosides include genistein, daidzein, and glycitein, and glycosides include genistin, daidzin, and glycitin. ), etc. While there are many glycosides in non-fermented soybean-derived foods, non-glycosides are mainly present in fermented foods using soybeans, such as soybean paste, as sugars are decomposed from glycosides by bacteria involved in fermentation.

Isoflavone derivatives in soybean have high affinity for estrogen receptor beta (ER-beta), making them the most effective estrogen substitutes (Endocrinology 1997, 863).

A lot of research has been conducted to develop soybean plants high in isoflavone derivatives, and recently, cultivation method technology for producing soybean leaves high in isoflavone derivatives by treating soybean leaves with ethylene or ethene, an ethylene donor (Korean Registered Patent No. 10-1451298, Korean Registered Patent No. 10-2282625) have been reported. These technologies treat the ethylene donor (ethephon) from soybeans grown in the open field about 50 to 60 days after soybean sowing, or harvest only the above-ground part of what is grown in the open field, move it to a closed treatment plant (container), and treat ethylene. (Temperature, rainy season, etc.) is not controlled, so it is difficult to stably produce standardized soybean plants containing high isoflavones, and it is impossible to produce soybean plants once or twice a year, and there is a problem that a lot of labor is required during production. there is.

Therefore, it has been required to develop a method for growing soybean plants containing high isoflavone derivatives that can be produced indoors all year round and requires less labor.

Accordingly, the present inventors developed a smart-metabolite chamber, confirmed that soybean plants containing high isoflavone derivatives could be grown indoors all year round, and completed the present invention.

An object of the present invention is to provide a novel smart-metabolite chamber capable of growing soybean plants enriched with isoflavone derivatives.

Another object of the present invention is to provide a method for growing soybean plants enriched with isoflavone derivatives using the smart-metabolite chamber.

Another object of the present invention is to provide soybean plants enriched with isoflavone derivatives grown by the above method.

Another object of the present invention is to provide a health functional food containing soybean plants enriched with the isoflavone derivatives.

Another object of the present invention is to provide cosmetics containing soybean plants enriched with the isoflavone derivatives.

Another object of the present invention is to provide a pharmaceutical composition comprising a soybean plant enriched with the isoflavone derivative.

In order to achieve the object of the present invention, a novel smart-metabolite chamber capable of growing soybean plants enriched with isoflavone derivatives is provided.

The smart-metabolite chamber includes a chamber part including an inner space; a door unit opening and closing the chamber unit; an observation window installed in the center of the door unit; An LED light source unit installed on the ceiling inside the chamber unit; temperature, humidity and carbon dioxide meters; Ventilation fan for ventilation of the chamber; A temperature control fan installed on the back of the inside of the chamber; ethylene gas injector; ethylene gas meter; Light source control device for controlling the LED light source; Automatic temperature and humidity control and data collection device; Water circulator for temperature control; ethylene gas cylinder; and data storage (see FIG. 1A).

According to another object of the present invention, the present invention provides a method for growing soybean plants enriched with isoflavone derivatives of Formula 1 using the smart-metabolite chamber:

[화학식 1]

[Formula 1]

In the above formula, R ₁ is H or OH, and R ₂ is glucose or glucose malonate.

In the present invention, the isoflavone derivative of Chemical Formula 1 may be any one or more of the compounds represented by [Formula 2] to [Formula 7] below.

In the present invention, the bean plant may be a bean leaf, a bean stalk, and/or a bean root.

The cultivation method

i) sowing soybean seeds or germinated soybeans in a smart-metabolite chamber and growing them for 20 to 40 days;

ii) spraying ethylene from an ethylene gas injector to treat soybean plants grown with ethylene at a concentration of 1,000 to 10,000 ppm for 12 to 72 hours; and

iii) harvesting soybean plants;

Stage i) Growth

Soybean seeds or germinated soybeans are sown in the smart-metabolite chamber and grown for 20 to 40 days.

In step i), soybean seeds or germinated soybeans may be used for sowing, and germinated soybeans are preferably sowed.

In the present invention, the germinated beans are put in 1 to 3 times the weight of the beans, soaked at 15 to 25 ° C, put in a bean sprouter or bean sprout maker at 20 to 30 ° C, and germinated within 16 to 24 hours in a dark room can be made into

In step i), the growth of soybean plants is performed 20 to 40 days after sowing, preferably 30 to 35 days. In the case of less than 20 days, the soybean plant biomass content is low, and in the case of more than 40 days, problems may arise in growth in the indoor smart-metabolite chamber.

The LED light source installed on the ceiling inside the chamber for the growth of bean plants includes a single light source of white, blue, and red; Dual mixed light sources of white and blue, white and red, and blue and red; Alternatively, a triple mixed light source of white, blue, and red may be used, and a triple mixed light source of white, blue, and red is preferable.

The amount of light in the smart-metabolite chamber for soybean plant growth is 100 μmol/m ² /s or more, preferably 200 μmol/m ² /s or more. If less than 100 μmol / m ² /s, photosynthesis is not smooth, so the soybean plant biomass content is low and the production of isoflavone derivatives is not smooth.

The temperature in the smart-metabolite chamber for soybean plant growth is preferably 20 to 35 ° C, more preferably 25 to 30 ° C. In the case of less than 20 ° C., the soybean plant does not grow due to cold damage to the soybean plant, and in the case of more than 35 ° C., high temperature failure may occur.

The humidity in the smart-metabolite chamber for soybean plant growth is preferably 40 to 80%, more preferably 60 to 70%. In the case of less than 40%, photosynthesis of soybean plants is not smooth due to lack of moisture, so that the leaves may wither, and in the case of more than 80%, the roots may rot due to excessive moisture.

step ii) ethylene treatment

Soybean plants grown by spraying ethylene from an ethylene gas sprayer are treated with ethylene at a concentration of 1,000 to 10,000 ppm for 12 to 72 hours.

Ethylene is the simplest olefinic hydrocarbon (chemical formula: C ₂ H ₄ ) and is a colorless, sweet gas. As a kind of plant hormone, it has the effect of promoting the maturation of plant tissues, such as physiological changes after harvest of fruits and vegetables, especially maturation of horticultural crops and yellowing of leafy vegetables.

The ethylene treatment concentration is a high concentration of 1,000 to 10,000 ppm, preferably 5,000 to 1,0000 ppm. When the concentration of ethylene treatment is less than 1,000 ppm, accumulation of isoflavone derivatives is hardly achieved, and even when treatment concentration exceeds 10,000 ppm, there is no significant difference in accumulation of isoflavone derivatives.

The ethylene treatment time is preferably 12 to 72 hours, more preferably 24 to 48 hours. When the ethylene treatment time is less than 12 hours, the accumulation of isoflavone derivatives is hardly achieved, and even when the ethylene treatment time exceeds 72 hours, there is no significant difference in the accumulation of isoflavone derivatives.

In step ii), the ethylene treatment takes place in an enclosed space where the smart-metabolite chamber is completely closed. That is, the door of the smart-metabolite chamber is closed and the ventilation fan is closed to seal it, and then ethylene is treated.

If necessary, the soybean plants are maintained for 1 to 2 hours in an enclosed space as described above before ethylene treatment to stabilize them. It is for humidity control due to moisture generated by respiration of soybean plants.

During ethylene treatment, the temperature of the smart-metabolite chamber is preferably 20 to 40°C, more preferably 30 to 35°C. Humidity is preferably 60 to 90%, more preferably 70 to 80%. In the case of ethylene treatment temperature of 20℃ and humidity of less than 60%, the metabolism of soybean plants is not smooth, so isoflavone derivatives are hardly accumulated. Isoflavone derivatives are not made because the action is not smooth.

iii) harvest

In step ii), soybean plants treated with ethylene are harvested.

As described above, the cultivation method according to the present invention can significantly increase the content of isoflavone derivatives in soybean plants by treating the soybean plants with ethylene under specific conditions as in step ii) before harvesting. In the case of soybean plants grown according to the present invention (about 25,047 μg / g; Example 1), soybean plants grown in the field and then treated with ethylene in a smart-metabolite chamber (about 12,031 μg / g; Comparative Example 2) and Compared to soybean plants grown in the smart-replacement chamber and not treated with ethylene (about 15,956 μg/g; Comparative Example 1), the contents of isoflavone derivatives were significantly increased by 2.08 and 1.57 times, respectively (Table 1).

According to another object of the present invention, to provide soybean plants enriched with isoflavone derivatives grown by the above method.

In the present invention, the isoflavone derivative-enhanced soybean plant may be at least one selected from the group consisting of soybean leaves, soybean stems and soybean roots, and is preferably soybean leaves and/or soybean roots.

Soybean plants enriched with isoflavone derivatives according to the present invention contain 25,000 μg/g or more of isoflavone derivatives, soybean leaves contain 9,590 μg/g or more of isoflavone derivatives, and bean stems contain 3,300 μg/g or more of isoflavone derivatives. g or more, and soybean roots contain more than 12,130 μg/g of isoflavone derivatives (Table 5, FIG. 12).

The soybean plant enriched with isoflavone derivatives according to the present invention can be used as a material for food, cosmetics and pharmaceutical compositions by itself or after being extracted with a solvent.

In the present invention, water, alcohol, or a mixture thereof may be used as the extraction solvent. It is preferable to use C1 to C2 lower alcohol as the alcohol, and it is preferable to use ethanol or methanol as the lower alcohol.

As an extraction method, shaking extraction, Soxhlet extraction, or reflux extraction is preferably used, but is not limited thereto. Specifically, it is preferable to extract by adding 1 to 10 times (v/w) of the extraction solvent to the dried soybean plant, the extraction temperature is preferably 20 to 100 ° C, and the extraction time is preferably 10 to 48 hours. However, it is not limited thereto.

The extract may be dried after being concentrated under reduced pressure, and it is preferable to use a vacuum concentrator or a vacuum rotary evaporator for concentration under reduced pressure, but is not limited thereto. In addition, drying is preferably performed under reduced pressure, vacuum drying, boiling drying, spray drying or freeze drying, but is not limited thereto.

The extract may be further fractionated with an organic solvent, and after suspending the extract in water, fractionation may be performed using n-hexane, chloroform, ethyl acetate or butanol as an organic solvent, preferably using chloroform. A fraction may be obtained by repeating the fractionation process from the extract 1 to 5 times, preferably 3 times, and it is preferable to concentrate under reduced pressure after fractionation, but is not limited thereto.

According to another object of the present invention, the present invention provides a health functional food comprising soybean plants enriched with the isoflavone derivatives.

The health functional food according to the present invention is useful for preventing and improving diseases caused by estrogen deficiency, for antioxidant, and for skin care.

In the present invention, the disease caused by estrogen deficiency is any one selected from the group consisting of osteoporosis, heart disease, breast cancer, vulvovaginal disease, hyperlipidemia, skin aging, and facial flushing.

Since isoflavone derivatives have established physiological activities for preventing and improving diseases caused by estrogen deficiency, for antioxidant purposes, and for skin care, the soybean plant according to the present invention containing a high content of isoflavone derivatives is It can be usefully used as a health functional food as described above.

The health functional food according to the present invention may include isoflavone derivative-enhanced soybean plants (bean leaves, soybean stalks, soybean roots) as it is, or may be included together with other foods or food ingredients in the form of extracts or fractions as described above. .

In the present invention, 'health functional food' refers to a food prepared using raw materials or components having useful functions for the human body or nutrients that are easily deficient in daily meals, and means food that helps maintain the health of the human body.

In the present invention, the form and type of health functional food is not particularly limited. Specifically, it may be in the form of tablets, capsules, powders, granules, liquids and pills. Health functional foods may contain various flavors, sweeteners, or natural carbohydrates as additional ingredients. The sweetener may be a natural or synthetic sweetener, and examples of natural sweeteners include thaumatin, stevia extract, and the like. On the other hand, examples of synthetic sweeteners include saccharin and aspartame. In addition, natural carbohydrates may include monosaccharides, disaccharides, polysaccharides, oligosaccharides and sugar alcohols.

In the present invention, health functional food, in addition to the above additional ingredients, nutrients, vitamins, electrolytes, flavors, colorants, pectanes and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin , alcohol and the like may be further included. These components may be used independently or in combination.

In the present invention, the type of food is not particularly limited, and may be pills, tablets, capsules, tea, wire, flour foods, soups, dairy products, sauces, meat additives, beverages (pouches, drinks, etc.), but are limited thereto. It doesn't work.

Preferably, the food according to the present invention may be tea. The tea according to the present invention may be a mixed tea in which a soybean plant enriched with an isoflavone derivative according to the present invention and a herbal plant having a flavor are mixed. The herbal plant is preferably at least one selected from the group consisting of chamomile, lemongrass, rosehip, lavender, peppermint, fennel, rosemary, jasmine, hibiscus, roseflower, apple fruit, strawberry fruit, lemon fruit and orange flower. The tea or blended tea of the present invention may be processed into a form selected from the group consisting of flavored tea, tea bag, instant tea and can.

According to another object of the present invention, the present invention provides a cosmetic comprising a soybean plant enriched with the isoflavone derivative.

Cosmetics according to the present invention may include isoflavone derivative-enhanced soybean plants (bean leaves, soybean stalks, soybean roots) as they are, or may be included together with other cosmetic ingredients in the form of extracts or fractions as described above.

Cosmetics in the present invention may be in the form of lotion, ointment, gel, cream, patch, mask pack or spray, but are not limited thereto, and in addition, fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, Antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, It may contain any additives commonly used in cosmetics, such as pigments, hydrophilic or lipophilic actives, and lipid vesicles.

According to another object of the present invention, the present invention provides a pharmaceutical composition for preventing and treating diseases caused by estrogen deficiency, including soybean plants enriched with the isoflavone derivatives.

In the present invention, the disease caused by estrogen deficiency is any one selected from the group consisting of osteoporosis, heart disease, breast cancer, vulvovaginal disease, hyperlipidemia, skin aging, and facial flushing.

Since isoflavone derivatives are established as estrogen substitutes for the prevention and improvement of diseases caused by estrogen deficiency as described above, the soybean plant according to the present invention containing a high content of isoflavone derivatives is a pharmaceutical composition for use as described above useful as

The pharmaceutical composition according to the present invention may be formulated in the form of oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories and sterile injection solutions according to conventional methods, respectively. can Carriers, excipients and diluents that may be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose , microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations contain at least one or more excipients such as starch, calcium carbonate, sucrose, etc. Alternatively, it is prepared by mixing lactose and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, solutions for oral use, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included. . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for the suppository, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogeratin and the like may be used.

The pharmaceutical composition according to the present invention can be administered orally or parenterally, and when administered parenterally, it can be administered by external skin or intraperitoneal injection, intrarectal injection, subcutaneous injection, intravenous injection, intramuscular injection or intrathoracic injection injection method. can

The preferred dosage of the pharmaceutical composition according to the present invention varies depending on the condition and body weight of the patient, the severity of the disease, the type of drug, the route of administration and the period of administration, but can be appropriately selected by those skilled in the art. For desirable effects, the composition is preferably administered at 0.0001 to 1 g/kg per day, preferably at 0.001 to 200 mg/kg. The administration may be administered once a day or divided into several times.

According to the soybean plant cultivation method using the smart-metabolite chamber according to the present invention, soybean plants enriched with isoflavone derivatives can be grown in a short period of time (20 to 40 days). In addition, the cultivation method of the present invention can stably produce soybean plants containing high isoflavone derivatives throughout the year.

Soybean leaves, soybean stems, and soybean roots, which are soybean plants enriched with isoflavone derivatives according to the present invention, and extracts or fractions thereof can be used as estrogen substitutes.

Soybean leaves, soybean stems, and soybean roots, which are soybean plants enriched with isoflavone derivatives according to the present invention, and extracts or fractions thereof are pharmaceutical compositions for preventing and improving diseases caused by estrogen deficiency, for antioxidants, and for skin care , It is useful as a material for liver functional foods and cosmetics.

1A shows an example of a configuration diagram of a smart-metabolite chamber according to the present invention. Figure 1b is a picture showing the actual manufactured product of the smart-metabolite chamber according to the present invention.
Figure 2 is a picture showing the process of growing soybean plants in a smart-metabolite chamber according to the present invention. (A) The soybean plant on the 5th day after sowing, (B) the soybean plant on the 15th day of growth, (C) the soybean plant on the 30th day of growth, and (D) the soybean plant after ethylene treatment.
3 is an HPLC chromatogram analyzing isoflavone derivatives of soybean leaves among soybean plants. Figure 3a is an HPLC chromatogram for isoflavone derivative analysis of soybean leaves (Comparative Example 1) that were not treated with ethylene after being grown in a smart-metabolite chamber, and Figure 3b is a smart- Isoflavone derivative analysis HPLC chromatogram of soybean leaves treated with ethylene in the metabolite chamber (Comparative Example 2), and FIG. It is an HPLC chromatogram. a is daidzine, b is genistin, c is malonyl daidzine, d is malonyl genistin, e is daidzein, and f is genistein.
4 is an HPLC chromatogram for analyzing isoflavone derivatives of soybean stems among soybean plants. Figure 4a is an HPLC chromatogram for isoflavone derivative analysis of soybean stems of Comparative Example 1, Figure 4b is an HPLC chromatogram for isoflavone derivatives analysis of soybean stems of Comparative Example 2, and Figure 4c is isoflavones of soybean stems of Example 1 Analytical HPLC chromatogram.
5 is an HPLC chromatogram of isoflavone derivative analysis of soybean roots among soybean plants. Figure 5a is an HPLC chromatogram for isoflavone derivative analysis of soybean roots of Comparative Example 1, Figure 5b is an HPLC chromatogram for isoflavone derivative analysis of soybean roots of Comparative Example 2, and Figure 5c is isoflavones of soybean roots of Example 1 Derivative analysis HPLC chromatogram.
FIG. 6 shows the BPI spectrum of LC-ESI-TOF/MS of peak a in the HPLC chromatogram of FIG. 3c and the chemical formula of daidzine, a corresponding compound.
FIG. 7 shows the LC-ESI-TOF/MS BPI spectrum of peak b in the HPLC chromatogram of FIG. 3c and the chemical formula of the corresponding compound, Genistine.
FIG. 8 shows the BPI spectrum of LC-ESI-TOF/MS of peak c in the HPLC chromatogram of FIG. 3c and the chemical formula of the corresponding compound, malonyl dididzine.
FIG. 9 shows the LC-ESI-TOF/MS BPI spectrum of peak d in the HPLC chromatogram of FIG. 3c and the chemical formula of the corresponding compound, malonyl genistine.
FIG. 10 shows the BPI spectrum of LC-ESI-TOF/MS of peak e in the HPLC chromatogram of FIG. 3c and the chemical formula of the corresponding compound, daidzein.
FIG. 11 shows the BPI spectrum of LC-ESI-TOF/MS of peak f in the HPLC chromatogram of FIG. 3c and the chemical formula of the corresponding compound, genistein.
Figure 12 is a graph comparing the total isoflavone derivative content of soybean plants of Comparative Example 1, Comparative Example 2 and Example 1.

Hereinafter, the present invention will be described in detail with reference to drawings, examples and manufacturing examples. However, the drawings, examples, and manufacturing examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the drawings, examples, and manufacturing examples.

<Smart-metabolite chamber>

The smart-metabolite chamber of the present invention will be described in detail with reference to the drawings.

1A shows an example of a configuration diagram of a smart-metabolite chamber according to the present invention.

Referring to FIG. 1A, the smart-metabolite chamber 1 according to the present invention includes a chamber unit 10 including an inner space, an opening and closing door unit 20 for opening and closing the chamber unit, and an observation window installed in the center of the door unit. (21), an LED light source unit 103 installed on the ceiling inside the chamber unit, a temperature, humidity and carbon dioxide meter 105; A ventilation fan 106 for ventilation of the chamber unit, a temperature control fan 107 installed on the back of the inside of the chamber unit, an ethylene gas spraying device 110, an ethylene gas meter 111, a light source control device 101 for controlling the LED light source, 102), an automatic temperature and humidity control and data collection device 104, a water circulation device for temperature control 108, an ethylene gas cylinder 109, and a data storage device 112.

In the smart-metabolite chamber according to the present invention, the chamber unit 10 may have a width × length × height of 500 to 1200 mm × 400 to 1000 mm × 400 to 8000 mm, and a size of 700 mm × 500 mm × 500 mm desirable. The material of the chamber part 10 is preferably made of aluminum or stainless steel, wood, or polyacrylic plate, but more preferably made of aluminum or stainless steel.

The door part 20 allows the chamber part 10 to be opened and closed, and an observation window 21 is installed in the center so that the growth of the plant can be observed (FIG. 1B). The observation window 21 may be made of a material such as a transparent acrylic plate or glass.

The LED light source unit 103 may be installed on the ceiling inside the chamber unit and may irradiate plants with one or more types of light selected from the group consisting of red, blue, white, UV-A and UV-B. The amount of light can be adjusted from 1% to 100% for each LED light source in a dark room condition.

The light source control devices 101 and 102 for controlling the LED light source may be installed on any part of the outer surface of the chamber unit 10, and a three-channel light source control device of red (660 nm), blue (450 nm), and white. (101) and UV-A (375 nm) and UV-B (315 nm) light source control devices 102. The light source control device can adjust the amount of light of each LED light source from 1% to 100%.

The temperature, humidity and carbon dioxide meter 105 may be installed on one side of the inside of the chamber unit 10 and is connected to an automatic temperature and humidity control and data collection device 104 installed outside the chamber unit 10. Temperature can be measured from -10℃ to 60℃, and humidity can be measured from 0% to 100%. Carbon dioxide can be measured from 0 to 2,000 ppm.

One or more ventilation fans 106 may be installed to ventilate the chamber unit 10 .

The temperature control fan 107 is installed on the inner rear surface of the chamber part 10, functions to control the temperature of the inner space of the chamber part, and is connected to the water circulation device 108 for temperature control installed outside. The temperature in the smart-metabolite chamber 1 can be kept constant from 15° C. to 45° C., and the actual humidity in the smart-metabolite chamber 1 can be kept constant from 20% to 100%.

The ethylene gas injection device 110 is installed on one side of the inside of the chamber part 10 to inject ethylene to plants, and is connected to the ethylene gas cylinder 109 installed outside the chamber part 10. The concentration of ethylene gas inside the chamber unit 10 can be sprayed at a concentration of up to 10,000 ppm, and the ethylene gas is evenly sprayed into the smart-metabolite chamber through the spray device 110 that temporarily passes through water. Ethylene gas injection control is controlled by installing a gas switch (not shown).

The ethylene gas meter 111 is installed under the temperature control fan 107 to measure the ethylene gas concentration inside the chamber. A meter (not shown) of the ethylene gas meter is installed outside and measures the amount detected by the meter.

The data storage device 112 is installed outside the chamber unit 10 and is connected to the automatic temperature and humidity control and data collection device 104 . Data collection device 104 may be a computer.

The smart-metabolite chamber (1) includes a power supply (DC24VDC) for power supply, a voltage divider (VDIV10.1) as a voltage drop module, a modem for remote access to the data logger (Ethernet modem), and wireless Wifi modem communication A router, which is a wired/wireless sharing device, and an electric controller for operating a temperature control fan/ventilation fan can be additionally installed.

Figure 1b is a picture showing the actual manufactured product of the smart-metabolite chamber according to the present invention.

Preparation Example 1: Cultivation of soybean plants in a smart-metabolite chamber

After adding water about twice the weight of the bean seeds and soaking the beans at 20 ° C, germinated beans (germinated beans) were prepared by putting them in a bean germination machine at 25 ° C and germinating for 20 hours in a dark room.

After filling the plant pot with general horticultural medium, 2 to 3 germinated beans were sown. The light ratio of white, blue, and red light of the smart-metabolite chamber 1 is set to 1: 1: 1, the amount of light is set to 400 µmol/m ² /s, the temperature is 25 ± 0.5 ° C and humidity After setting to 65 ± 5%, the soybean photos sowed above were placed in a smart-metabolite chamber and grown for 30 days (Fig. 2 (A) to (C), Comparative Example 1).

Ethylene treatment in the smart-metabolite chamber (1) was performed in an enclosed space completely blocked. After completely blocking the ventilation fan of the smart-metabolite chamber, make it an airtight space, stabilize it for 2 hours under the condition of temperature 35 ± 1 ℃ and humidity 80 ± 5%, and then spray ethylene gas through the ethylene gas injection device 110 After treating the grown soybean plants with ethylene at a concentration of 10,000 ppm for 24 hours, the soybean plants were harvested (FIG. 2 (D); Example 1). Harvested soybean plants were washed three times in running water, separated into leaves, stems, and roots, dried at 50 ± 2 ° C for 24 to 48 hours, and stored in a refrigerator.

For comparison, soybeans were sown in the field (open field), grown for 60 days, harvested in the field, moved to the smart-metabolite chamber (1), and treated with ethylene in the same manner as above to treat ethylene in the field Soybean plants were prepared (Comparative Example 2).

Reference Example: Preparation of soybean plant extract

The soybean leaves, soybean stems, and soybean roots of the prepared soybean plants were dried, and 400 ml of 50% ethanol was added to 10 g of each of the dried soybean leaves, soybean stems, and soybean roots, and extracted at 25° C. for 5 days. The extracted solvent was concentrated under reduced pressure to obtain extracts of soybean leaves, soybean stems, and soybean roots in a yield of 16 to 20%.

Experimental Example 1: Analysis of isoflavone derivatives of soybean leaves, soybean stems, and soybean roots

High performance liquid chromatography (HPLC) analysis was performed to confirm the degree of accumulation of isoflavone derivatives in soybean leaves, soybean stems, and soybean roots of Example 1, Comparative Example 1, and Comparative Example 2.

Each extract prepared in the reference example was filtered using a membrane filtration filter having a pore size of 0.2 μm, and then isoflavones were analyzed. For HPLC, an Agilent 1260 system LC from Agilent Technologies Inc., Waldbronn, Germany was used, and a Lichrophore 100 RP C18 column (4.6 × 250 mm, 5 μm, Merck, Germany) was used as the column. The elution rate is 1 ml / min, and the elution conditions are 0.2% acetic acid aqueous solution (solvent A) and acetonitrile (solvent B) mixed solution with 0.2% acetic acid added. Gradient elution as shown in Table 1 is applied. A flavone derivative was isolated. At this time, analysis was performed at a detection wavelength of 254 nm by a diode array detector (DAD) detector, and the results are shown in FIGS. 3A to 5C.

time (minutes) Solvent A (%) Solvent B (%) 0 100 0 15 90 10 25 80 20 35 75 25 45 65 35 50 65 35

3a to 5c, the isoflavone derivative content of Comparative Example 1 (soybean plants grown in the smart-metabolite chamber and not treated with ethylene) was almost low, but Example 1 (smart-metabolite chamber In the case of soybean plants grown and treated with ethylene), it can be seen that 4 to 6 isoflavone derivatives are significantly accumulated. treated soybean plants), 4 to 6 isoflavone derivatives were significantly increased.

Specifically, in the case of the bean leaves of Comparative Example 1, the isoflavone derivative peak was low (FIG. 3a), but in the case of the bean leaf of Example 1, it was confirmed that 6 types of isoflavone derivatives were significantly increased (FIG. 3c). The soybean leaf of 2 (FIG. 3b) was significantly increased. In addition, the soybean stem of Comparative Example 1 had a low isoflavone derivative peak (FIG. 4a), but the soybean stem of Example 1 showed a significant increase in 6 isoflavone derivatives (FIG. 4c), and the soybean stem of Comparative Example 2 (FIG. 4b) it can be confirmed that it is more increased. On the other hand, four isoflavone derivatives (daidzin, genistin, malonyl daidzin, and malonyl genistin) were significantly increased in the bean root of Example 1 compared to the bean root of Comparative Example 1 (FIG. 5a) (FIG. 5c). ), four isoflavone derivatives (daidzin, malonyl dididzin, daidzein and genistein) were significantly increased compared to the soybean root of Comparative Example 2 (FIG. 5b) (FIG. 5c).

In the case of soybean leaves, soybean stems, and soybean roots of Example 1, daidzin (peak a), genistin (peak b), malonyl daidzin (peak c) and malonyl genistin (peak d) were detected at very high concentrations (Fig. 3c, Fig. 4c, Fig. 5c), in the case of the bean leaves and bean stems of Example 1, daidzein (peak e) and genistein (peak f) were detected at higher concentrations than those of the bean leaves and bean stalks of Comparative Example 1 (FIGS. 3c and 4c), and in the case of the bean root of Example 1, higher concentrations of daidzein (peak e) and genistein (peak f) were detected than the bean root of Comparative Example 2 (Fig. 5c).

From the above results, it can be confirmed that according to the method of growing soybean plants treated with ethylene by growing in a smart-metabolite chamber according to the present invention, soybean plants containing high concentrations of isoflavone derivatives can be obtained.

Experimental Example 2: Isolation, Purification and Structural Analysis of Isoflavone Derivatives

UPLC-ESI-TOF/MS (Acquity UPLC coupled system with SYNAPT G2-Si HDMS, Waters) analysis was performed on the isoflavone derivatives corresponding to each peak of the soybean leaf (FIG. 3c) of Example 1.

Specifically, a UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm, water) was used for the extract prepared in the reference example, and the elution conditions were 0.1% formic acid aqueous solution (solvent A) and 0.1% formic acid acetonitrile (solvent B ) Isoflavone derivatives were isolated by applying gradient elution as shown in Table 2 to the mixed solution. At this time, the amount of the injected extraction solution was 1 μl, and UPLC-ESI-TOF mass spectrometry was performed under the same conditions as in Table 3, and the results are shown in FIGS. 6 to 12.

time (minutes) Solvent A (%) Solvent B (%) 0 7 3 5 85 15 10 75 25 11 70 30 12 55 45

Detail condition Ionization ESI-negative mode Capillary voltage 3 kV Sample cone voltage 40V Desolvation gas flow 800 L/h Cone gas flow 30L/h Desolvation temperature 400℃ Ion source temperature 100℃ Data collection m/z 50-1500 rage with a scan time of 0.2 s Lock mass Leucine-enkephalin (556.2771 Da)

As shown in FIG. 6, in the mass spectrometry spectrum of peak a in FIG. 3c, [M+H] ^- was 415.0 (theoretical molecular weight: 416.3). From this, it was confirmed that the elemental composition was C ₂₁ H ₂₁ O ₉ consistent with the elemental composition of daidzin. Peak a was identified as daidzin of Formula 2, as it coincided with daidzin shown in the reference literature (J. Agric. Food Chem. 2016, 64, 7315-7324).

As shown in FIG. 7, in the mass spectrometry spectrum of peak b in FIG. 3c, [M+H] ^- was 431.1 (theoretical molecular weight: 432.3). From this, it was confirmed that the elemental composition was C ₂₁ H ₂₁ O ₁₀ consistent with the elemental composition of Genistine. Peak a was identified as Genistin of Chemical Formula 3 because it coincided with Genistin shown in the reference document (J. Agric. Food Chem. 2016, 64, 7315-7324).

As shown in FIG. 8, in the mass spectrometry spectrum of peak c in FIG. 3c, [M+H] ^- was 501.4 (theoretical molecular weight: 502.4). From this, it was confirmed that the elemental composition was C ₂₄ H ₂₃ O ₁₂ consistent with the elemental composition of malonyl daidzin. Peak c was identified as malonyl daidzin of Chemical Formula 4, as it coincided with malonyl daidzin shown in the reference literature (J. Agric. Food Chem. 2016, 64, 7315-7324).

As shown in FIG. 9, in the mass spectrometry spectrum of peak d in FIG. 3c, [M+H] ^- was 517.1 (theoretical molecular weight: 518.4). From this, it was confirmed that the elemental composition was C ₂₄ H ₂₃ O ₁₃ consistent with the elemental composition of malonyl genistin. Peak a was identified as malonyl genistin of formula 5, as it coincided with malonyl genistin shown in the reference literature (J. Agric. Food Chem. 2016, 64, 7315-7324).

As shown in FIG. 10, in the mass spectrometry spectrum of peak e in FIG. 3c, [M+H] ^- was 253.1 (theoretical molecular weight: 254.2). From this, it was confirmed that the elemental composition was C ₁₅ H ₁₀ O ₄ consistent with the elemental composition of malonyl daidzin. Peak c was identified as malonyl daidzin of Chemical Formula 6, as it coincided with malonyl daidzin shown in references (Food Chem. 2020, 305, 125462).

As shown in FIG. 11, in the mass spectrometry spectrum of peak f in FIG. 3c, M+H] ^- was 269.0 (theoretical molecular weight: 270.4). From this, it was confirmed that the elemental composition was C ₁₅ H ₁₀ O ₅ consistent with the elemental composition of malonyl genistin. Peak a coincided with malonyl genistin shown in the reference literature (Food Chem. 2020, 305, 125462), so it was identified as malonyl genistin of formula 7.

Experimental Example 3: Analysis and comparison of content of isoflavone derivatives in soybean plants

Each extract of Example 1, Comparative Example 1, and Comparative Example 2 was filtered using a membrane filtration filter having a pore of 0.2 μm, and then the isoflavone content was analyzed and the total amount was quantified and compared.

Specifically, for HPLC, an Agilent 1260 system LC from Agilent Technologies Inc., Waldbronn, Germany was used, and a Lichrophore 100 RP C18 column (4.6 × 250 mm, 5 μm, Merck, Germany) was used as the column. . The solvent flow rate was adjusted to 1 ml/min, the amount of the injected extraction solution was 20 μl, and the mobile phase was a mixture of 0.2% acetic acid aqueous solution (solvent A) and acetonitrile (solvent B) to which 0.2% acetic acid was added, as shown in Table 4. Isoflavone derivatives were isolated by applying gradient elution. At this time, the DAD (diode array detector) detector was analyzed at a detection wavelength of 254 nm. Standard solutions used for quantitative analysis were prepared by dissolving 98% pure daidzine, genistin, daidzein, and genistein purchased from Sigma (USA) in methanol to have a concentration of 1 to 100 μg/ml. HPLC analysis was performed with the prepared standard solution, and a calibration curve was prepared from the peak area.

time (minutes) Solvent A (%) Solvent B (%) 0 100 0 15 90 10 25 80 20 35 75 25 45 65 35 50 65 35

The content and total amount of each isoflavone derivative of the soybean plants analyzed as above are shown in Table 5:

content (μg/g) Ethylene-free ethylene treatment Comparative Example 1 Comparative Example 2 Example 1 bean leaves bean stalk bean root bean leaves bean stalk bean root bean leaves bean stalk bean root Glycosides Daidzin (a) 683.16 185.11 220.24 1236.73 590.23 292.88 1550.28 406.68 868.85 Genistine (b) 402.41 11.01 14.46 883.10 224.99 39.25 1449.34 78.74 43.79 Sum 1085.57 196.12 234.7 2119.82 815.22 332.13 2999.62 485.42 912.64 Malonly-glycosides Daidzin (c) 475.07 761.33 529.50 2644.20 875.54 500.51 2692.37 1,827.62 1908.42 Genistin (d) 1089.32 239.14 104.77 2571.35 554.72 108.64 3200.12 812.52 106.93 Sum 1564.39 1000.47 634.27 5215.55 1430.25 609.15 5892.49 2,640.14 2015.35 Aglycones Daidzein (e) 431.79 107.83 9,645.31 220.91 349.24 483.16 452.76 138.30 8467.71 Genistein (f) 206.11 27.15 822.96 167.18 72.43 86.29 251.12 48.05 743.74 Sum 637.90 134.98 10468.27 518.16 421.67 569.45 703.88 186.35 9211.45 All experiments were repeated 5 times and expressed as the average value.
nd: not detected.

As shown in Table 5, in the case of the soybean leaves of Example 1, daidzin was 1550.28 μg/g, genistin was 1449.34 μg/g, malonyl daidzin was 2692.37 μg/g, and malonyl genistin was 3200.12 μg/g. 2.3 times, 3.6 times, 5.7 times and 2.9 times respectively compared to the bean leaves of Comparative Example 1, and in the case of the bean stems of Example 1, daidzin was 406.68 μg/g, genistin was 78.74 μg/g, and malonyl da Idgin was 1827.62 μg/g and malonyl genistin was 812.52 μg/g, respectively, 2.2 times, 7.2 times, 2.4 times, and 3.4 times higher than the soybean stem of Comparative Example 1. In the case of the bean root of Example 1, daidzin was 868.85 μg/g, genistin was 43.79 μg/g, and malonyl daidzin was 1908.42 μg/g, respectively, 2.2 times, 7.2 times, and 2.4 times higher than that of the bean root of Comparative Example 1. doubled.

On the other hand, compared to the bean leaves of Comparative Example 2, the bean leaves of Example 1 increased daidzin, genistin, malonyl genistin, daidzein, and genistein by 1.2 times, 16 times, 1.2 times, 2.1 times, and 1.5 times, respectively. Compared to 2, in the case of the bean stem of Example 1, malonyl daidzin and malonyl genistin increased by 2.1 times and 1.5 times, respectively, and in the case of the bean root of Example 1, daidzin, malonyl daidzin, daidzein and genistein significantly increased by 3.0-fold, 3.8-fold, 17.5-fold, and 8.6-fold, respectively, compared to Comparative Example 2.

The total isoflavone content of each soybean plant was calculated and shown in FIG. 12 .

As shown in FIG. 12, the total isoflavone contents of the bean leaves and bean stems of Example 1 were 9595.99 μg/g and 3311.91 μg/g, respectively, and significantly increased by 2.9 and 2.5 times compared to the bean leaves and bean stems of Comparative Example 1. did In addition, in the case of Example 1, the total isoflavone content of soybean leaves and soybean stems of Comparative Example 2 was 1.2 times higher, respectively, but in the case of soybean roots, it was significantly higher at 8.0 times.

Preparation Example 2: Preparation of pharmaceutical composition

<1-1> Preparation of powder

0.1 g of soybean leaves, soybean stalks and soybean roots enriched with isoflavone derivatives of the present invention, extracts thereof or fractions of extracts, lactose 1.5 g and talc 0.5 g

A powder was prepared by mixing the above components and filling in airtight bags.

<1-2> Manufacture of tablets

0.1 g of soybean leaves, soybean stems and soybean roots, extracts or fractions of extracts enriched with the isoflavone derivatives of the present invention, 7.9 g of lactose. 1.5 g of crystalline cellulose and 0.5 g of magnesium stearate

After mixing the above components, tablets were prepared by direct tableting method.

<1-3> Preparation of capsules

Soybean leaves, soybean stalks and soybean roots enriched with the isoflavone derivatives of the present invention, 0.1 g of extracts or fractions of extracts thereof, 5 g of corn starch and 4.9 g of carboxy cellulose

After preparing a powder by mixing the above ingredients, the powder was filled into a hard capsule according to a conventional capsule preparation method to prepare a capsule preparation.

<1-4> Preparation of injections

Soybean leaves, soybean stems and soybean roots enriched with the isoflavone derivatives of the present invention, 0.1 g of extracts or fractions thereof, appropriate amount of sterilized distilled water for injection, appropriate amount of pH adjusting agent

It was prepared with the above component content per 1 ampoule (2 ml) according to the conventional method for preparing injections.

<1-5> Preparation of liquid formulation

Soybean leaves, soybean stems and soybean roots enriched with the isoflavone derivatives of the present invention, 0.1 g of extracts or fractions thereof, 10 g of isomerized sugar, 5 g of mannitol, appropriate amount of purified water

Each component was added to and dissolved in purified water according to a conventional method for preparing a liquid formulation, an appropriate amount of lemon flavor was added, and the above components were mixed. Then, purified water was added to adjust the total volume to 100 ml, and then filled into a brown bottle and sterilized to prepare a solution.

Preparation Example 3: Preparation of health functional food

<1-1> Manufacturing of blended tea

Blended tea was prepared by mixing soybean leaves, soybean stems and soybean roots, extracts thereof or fractions of extracts enriched with the isoflavone derivatives of the present invention, and herb plants having flavor.

<1-2> Manufacture of flour food

0.5 to 5.0 parts by weight of soybean leaves, soybean stems and soybean roots enriched with the isoflavone derivatives of the present invention, extracts thereof or fractions of the extracts are added to wheat flour, and breads, cakes, cookies, crackers and noodles are prepared using the mixture. manufactured.

<1-3> Manufacture of soups and gravies

0.1 to 5.0 parts by weight of soybean leaves, soybean stalks and soybean roots, extracts thereof or fractions thereof enriched with the isoflavone derivatives of the present invention were added to soups and broths to prepare health-promoting meat products and noodle soups and broths.

<1-4> Manufacture of ground beef

Ground beef for health promotion was prepared by adding 10 parts by weight of soybean leaves, soybean stems and soybean roots, extracts thereof or fractions thereof enriched with the isoflavone derivatives of the present invention to ground beef.

<1-5> Manufacture of dairy products

5 to 10 parts by weight of soybean leaves, soybean stalks and soybean roots enriched with the isoflavone derivatives of the present invention, their extracts or fractions of the extracts were added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<1-6> Manufacture of wire

Brown rice, barley, glutinous rice, and adlay were alphanized by a known method, dried, roasted, and then prepared into a powder having a particle size of 60 mesh using a grinder.

Black beans, black sesame seeds, and perilla seeds were also steamed and dried by a known method, roasted, and then prepared into powder with a particle size of 60 mesh by a grinder.

The isoflavone derivative-enhanced soybean leaves, soybean stems and soybean roots, extracts thereof, or fractions of the extracts are concentrated under reduced pressure in a vacuum concentrator, dried with a spray or hot air dryer, and the dried product is pulverized with a grinder to a particle size of 60 mesh and dried. powder was obtained.

It was prepared by blending the grains, seeds, and isoflavone derivative-enhanced soybean leaves, soybean stalks and soybean roots, extracts thereof or fractions of extracts of the present invention in the following ratio.

Cereals (30 parts by weight of brown rice, 15 parts by weight of adlay, 20 parts by weight of barley), seeds (7 parts by weight of perilla seeds, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds), soybean leaves enriched with isoflavone derivatives of the present invention, soybeans Stems and bean roots, extracts thereof or fractions of extracts (3 parts by weight), ganoderma lucidum (0.5 parts by weight), Rehmannia Root (0.5 parts by weight)

<1-7> Manufacture of beverages

Soybean leaves, soybean stems and soybean roots enriched with the isoflavone derivatives of the present invention, 100 mg of extracts or fractions thereof, 100 mg of citric acid, 100 mg of oligosaccharides, 2 mg of plum concentrate, 100 mg of taurine, and purified water were added to make a total of 500 ml.

After mixing the above components according to the usual health drink manufacturing method, stirring and heating at 85 ° C. for about 1 hour, the resulting solution is filtered and obtained in a sterilized 1 L container, sealed and sterilized, and stored in a refrigerator according to the present invention. It is used in the manufacture of health beverage compositions.

Although the composition ratio is a mixture of ingredients suitable for a relatively favorite beverage in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as class of demand, country of demand, and purpose of use.

Preparation Example 4: Cosmetics Manufacturing

<1-1> Cream manufacturing

Soybean leaves, soybean stalks and soybean roots enriched with isoflavone derivatives of the present invention, 4.6 parts by weight of extracts or fractions thereof, 2.8 parts by weight of cetostearyl alcohol, 2.6 parts by weight of beeswax, 1.4 parts by weight of stearic acid, lipophilic monostearate glycerin 2 parts by weight, PEG-100 stearate 1 part by weight, sesquioleate 1.4 parts by weight, jojoba oil 4 parts by weight, squalane 3.8 parts by weight, polysorbate 60 1.1 parts by weight, macadamia oil 2 parts by weight, tocopherol acetate 0.2 parts by weight, methylpolysiloxane 0.4 parts by weight, ethylparaben 0.1 parts by weight, propylparaben 0.1 parts by weight, Euxyl K-400 0.1 parts by weight, 1,3-butylene glycol 7 parts by weight, methylparaben 0.05 parts by weight, glycerin 6 parts by weight 0.2 parts by weight of d-panthenol, 0.2 parts by weight of triethanolamine, 0.2 parts by weight of pt 41891, and 0.295 parts by weight of p-H O 46.05 were mixed and prepared according to the cream manufacturing method.

<1-2> Manufacture of Lotion

3.5 parts by weight of extracts or fractions of extracts of soybean leaves, soybean stalks, and soybean roots enriched with the isoflavone derivatives of the present invention, 1.6 parts by weight of cetostearyl alcohol, 1.4 parts by weight of stearic acid, 1.8 parts by weight of lipophilic monostearate glycerin, PEG -100 stearate 2.6 parts by weight, sesquioleate 0.6 parts by weight, squalene 4.8 parts by weight, macadamia oil 2 parts by weight, jojoba oil 2 parts by weight, acetic acid tocopherol 0.4 parts by weight, methylpolysiloxane 0.2 parts by weight, ethylparaben 0.1 parts by weight part, 0.1 part by weight of propyl paraben, 4 part by weight of 1,3-butylene glycol, 0.1 part by weight of methylparaben, 0.1 part by weight of xanthan gum, 4 parts by weight of glycerin, 0.15 part by weight of d-panthenol, 0.1 part by weight of allantoin, carbohydrate (2% aq. Sol) 4 parts by weight, triethanolamine 0.15 parts by weight, ethanol 3 parts by weight, pt 41891 0.1 parts by weight, p-H20 48.3 parts by weight were mixed and prepared according to the lotion manufacturing method.

1: Smart-metabolite chamber
10: chamber part
20: door part
21: observation window
101: light source control device (red, blue, white light source)
102: light source control device (UV-A or UV-B)
103: LED light source (red/blue/white/UV-A or B)
104: temperature and humidity automatic control and data collection device
105: temperature, humidity and carbon dioxide meter
106: ventilation fan
107: temperature control fan
108: water circulation device for temperature control
109: ethylene gas cylinder
110: ethylene gas injector
111: ethylene gas meter
112: data storage

Claims (17)

a chamber portion including an inner space; a door unit opening and closing the chamber unit; an observation window installed in the center of the door unit; An LED light source unit installed on the ceiling inside the chamber unit; temperature, humidity and carbon dioxide meters; Ventilation fan for ventilation of the chamber; A temperature control fan installed on the back of the inside of the chamber; ethylene gas injector; ethylene gas meter; Light source control device for controlling the LED light source; Automatic temperature and humidity control and data collection device; Water circulator for temperature control; ethylene gas cylinder; and a smart-metabolite chamber for growing soybean plants enriched with isoflavone derivatives comprising a data storage device.
A method for cultivating soybean plants enriched with isoflavone derivatives of Formula 1 using the smart-metabolite chamber according to claim 1,

[Formula 1]
In the above formula, R ₁ is H or OH, R ₂ is glucose or glucose malonate,
The above method
i) sowing soybean seeds or germinated soybeans in a smart-metabolite chamber and growing them for 20 to 40 days;
ii) spraying ethylene from an ethylene gas injector to treat soybean plants grown with ethylene at a concentration of 1,000 to 10,000 ppm for 12 to 72 hours; and
iii) harvesting soybean plants; a method comprising the.
The method for cultivating soybean plants enriched with isoflavone derivatives according to claim 2, wherein the sowing in step i) uses germinated soybeans.
The method of claim 2, wherein in step i), the soybean plant is grown 30 to 35 days after sowing.
The method of claim 2, wherein in step i), the light source of the smart-metabolite chamber is a triple mixed light source of white, blue, and red, the amount of light is 100 μmol / m ² /s or more, the temperature is 20 ~ 35 ℃, humidity Is 40 to 80%, isoflavone derivatives fortified soybean plant cultivation method.
The method of claim 2, wherein the ethylene concentration in step ii) is 5,000 to 1,0000 ppm.
The method of claim 2, wherein the ethylene treatment time in step ii) is 24 to 48 hours.
The method of claim 2, wherein in step ii), the temperature of the smart-metabolite chamber is 20-40 ° C and the humidity is 60-90%.
Soybean plants enriched with isoflavone derivatives prepared by the cultivation method according to claim 2.
The isoflavone derivative-enriched soybean plant according to claim 9, wherein the soybean plant is any one selected from the group consisting of soybean leaves, soybean stems, and soybean roots.
The soybean plant enriched with isoflavone derivatives according to claim 9, wherein the isoflavone derivative is one or more selected from the group consisting of the following formulas 2 to 6:

11. The method according to claim 10, wherein the soybean leaves contain at least 9,590 μg/g of isoflavone derivatives, the soybean stalks contain at least 3,300 μg/g of isoflavone derivatives, and the soybean roots contain at least 12,130 μg/g of isoflavone derivatives. Soybean plants enriched with isoflavone derivatives.

A pharmaceutical composition for the prevention and treatment of diseases caused by estrogen deficiency, containing soybean plants enriched with the isoflavone derivative according to claim 9 as an active ingredient,
The disease caused by estrogen deficiency is osteoporosis, heart disease, breast cancer, vulvovaginal disease, hyperlipidemia, skin aging and facial flushing any one selected from the group consisting of a pharmaceutical composition.
A health functional food for improving diseases caused by estrogen deficiency, containing soybean plants enriched with isoflavone derivatives according to claim 9 as an active ingredient,
The disease caused by estrogen deficiency is any one selected from the group consisting of osteoporosis, heart disease, breast cancer, vulvovaginal disease, hyperlipidemia, skin aging and facial flushing.
A health functional food for antioxidant containing soybean plants enriched with isoflavone derivatives according to claim 9 as an active ingredient.
[Claim 16] The health functional food for antioxidant according to claim 15, wherein the food is tea.
Cosmetics containing soybean plants enriched with isoflavone derivatives according to claim 9 as an active ingredient.

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