KR20230067746A - Liquid composition and feed composition containing lutein as an active ingredient - Google Patents

Abstract

The present invention relates to a liquid composition containing lutein, which is insoluble in water and oil, as an active ingredient, and to the use of the liquid composition as feed. Lutein is, due to poorly soluble nature, currently sold only as products contained in pills or capsules. However, the present invention has identified the optimal formation for stabilizing lutein in a liquid state, and the liquid composition prepared with the optimal formation has stable properties under various temperature conditions and does not cause precipitation, to be usefully used as a health food for eye health or as feed for pets.

Description

Liquid composition and feed composition containing lutein as an active ingredient {Liquid composition and feed composition containing lutein as an active ingredient}

The present invention relates to a liquid composition containing lutein, which is insoluble in water and oil, as an active ingredient, and a feed use of the liquid composition.

In recent years, the number of households raising pets in Korea is increasing considerably because not only the family structure has become nuclear, but also because breeding pets helps emotional development. Pets are grown while being protected as family members, not animals that grow outside, but are frequently exposed to contaminated natural environments, such as air or water pollution, processed foods, and stress that humans experience. For this reason, various diseases, such as eye diseases, skin diseases, respiratory diseases, and digestive disorders, which are common in humans, have been developed in pets. Therefore, various feeds are currently being developed so that pets can lead a healthy life.

Meanwhile, lutein is one of xanthophylls, and is one of 600 naturally occurring carotenoids known to date. Lutein is synthesized only in plants and other xanthophylls, and is found in large quantities in leafy vegetables such as spinach, kale, and yellow carrots. The name is derived from the Latin luteus meaning yellow.

In addition, lutein is a nutrient that helps the health of tired eyes, and serves to protect the eyes from sun and oxidative damage. Since most mammals, including humans, cannot synthesize carotenoids, lutein can be consumed through foods containing lutein or dietary supplements. Intake of lutein increases the light density of macular pigment, improving visual function in patients with eye diseases, and effectively inhibits the aging process caused by damage to photoreceptors caused by harmful reactive oxygen species in the eye, preventing cataracts or age-related macular degeneration (Age). -related macular degeneration) can reduce the incidence of eye diseases ([Bernstein P.S et al., Ophthalmology, 2002(109), 1780-1787] and [Kvansakul J et al., Ophthalmic and Physiological Optics, 2006( 26), 362-371]). In addition, as lutein has been recognized as a safe supplement and health functional raw material (functional raw material recognition no. 2008-28, no. 59) by the U.S. Supplement Health Education Act (DSHEA) in 1994 and KFDA in 2008, respectively, lutein Health drinks, functional foods, and nutritional supplements are being actively developed.

However, lutein has poor water solubility and low solubility in fats and surfactants, making it difficult to design a liquid formulation in which the drug is dissolved. It is reported that there are problems that may cause gastrointestinal side effects ([Hentschel R1, Jorch G. Acute side effects of surfactant treatment. J Perinat Med. 2002;30(2):143-8] and [Aulton's Pharmaceutics E-Book: The Design and Manufacture of Medicines, Michael E. Aulton, Kevin M.G. Taylor, Elsevier Health Sciences, July 29, 2013], etc.).

Accordingly, the present inventors have made efforts to prepare a liquid composition containing lutein, and as a result, have identified an optimal composition for stabilizing lutein in a liquid phase. By confirming that this does not occur, the present invention was completed by revealing that the liquid composition containing lutein prepared through the optimal composition of the present invention can be usefully used as a health food or feed for pets.

Republic of Korea Patent Publication No. 10-2021-0096951

An object of the present invention is to provide a liquid composition containing lutein having poor water solubility as an active ingredient.

In order to achieve the above object, the present invention provides a liquid composition containing lutein, glycerin, an emulsifier, a binder, an oligosaccharide and water as active ingredients.

In addition, the present invention provides a health food for improving eye health containing the liquid composition as an active ingredient.

In addition, the present invention provides a feed composition containing the liquid composition as an active ingredient.

Due to its poor solubility, lutein is currently marketed only as a pill or capsule product. However, the present invention has identified an optimal composition for stabilizing lutein in a liquid phase, and a liquid composition prepared with the optimal composition can be prepared at various temperatures. It is stable in appearance and does not cause precipitation under the conditions, so it can be usefully used as a health food for eye health or as a feed for pets.

1A is a diagram confirming temperature stability at 4° C. of a liquid feed composition containing lutein and containing or not containing pullulan prepared in <Example 1> and <Example 2> of the present invention.
Figure 1b is a diagram confirming the temperature stability at 25 ℃ of the liquid feed composition containing lutein and containing or not containing pullulan prepared in <Example 1> and <Example 2> of the present invention.
1c is a diagram confirming the temperature stability at 40° C. of liquid feed compositions containing lutein and containing or not containing pullulan prepared in <Example 1> and <Example 2> of the present invention.
1D is a diagram confirming the temperature stability at 45° C. of liquid feed compositions containing lutein and containing or not containing pullulan prepared in <Example 1> and <Example 2> of the present invention.
Figure 2a is a diagram confirming the temperature stability of the liquid feed composition containing lutein and pullulan prepared in <Example 1> of the present invention under various temperature conditions.
Figure 2b is a diagram confirming the temperature stability of the liquid feed composition containing lutein and not containing pullulan prepared in <Example 2> of the present invention under various temperature conditions.

Hereinafter, the present invention will be described in detail.

The present invention provides a liquid composition containing lutein, glycerin, an emulsifier, a binder, an oligosaccharide, and water as active ingredients.

The lutein is a compound represented by Formula 1 and is known as a nutrient that helps the health of tired eyes, plays a role in protecting the eyes from damage caused by sun and oxidation, and is used in various health foods.

[Formula 1]

The emulsifier is glycerin fatty acid ester, lecithin, sodium metaphosphate, sorbitan fatty acid ester, sodium stearyl lactate, calcium stearyl lactate, sucrose fatty acid ester, polyoxyethylene glycerol fatty acid ester, polyoxyethylene sorbitan fatty acid ester, sodium polyphosphate And it is preferably any one or two or more selected from the group consisting of sodium stearyl lactate, but is not limited thereto.

In addition, the binder is a main component for stabilizing poorly soluble lutein in a liquid phase, and is preferably any one selected from the group consisting of natural binders, synthetic binders, and mixtures, and does not contain the binder. In this case, since the liquid phase stability of lutein is significantly impaired, a binder, particularly pullulan, should be included in order to prepare a stabilized liquid composition containing lutein. On the other hand, the natural binder is preferably any one selected from the group consisting of pullulan, guar gum, ligninsulfonate (ligninsulfonate), cellulose, rosin, gelatin, natural gum, casein, and collagen, and synthetic binder The agent is preferably any one selected from the group consisting of sodium alginate, ammonium alginate, potassium alginate, sodium carboxymethylcellulose, sodium caseinate, polymethylocarbamide, sodium polyacrylate and hydroxypropylmethyl cellulose, and the mixture is preferably a natural binder mixture, a synthetic binder mixture, and a binder mixture, but is not limited thereto.

In addition, the oligosaccharide is preferably any one selected from the group consisting of galactooligosaccharide, gentio-oligosaccharide, maltooligosaccharide, isomaltooligosaccharide, xylooligosaccharide, chitooligosaccharide, fructooligosaccharide and oligosaccharide mixture, but is not limited thereto. .

On the other hand, the liquid composition of the present invention may additionally include at least one selected from the group consisting of flavor, amino acid-based additives, vitamin-based additives, and functional additives, and the flavor enhances the taste of intake. Since it increases the ease of use, a variety of known natural flavoring agents and synthetic flavoring agents such as essential oil extracted from spices, essential oil extracted from citrus peel, coffee or vanilla bean extract, etc. can be used.

In addition, amino acid-based additives, vitamin-based additives, and functional additives that can help growth and development may be included together. DL-Methionine Hydroxide Derivative Calcium Salt, DL-Alanine, DL-Trainin Iron, DL-Tryptophan, L-Glutamic Acid, L-Lysine, L-Lysine Hydrochloride, L-Lysine Sulfate, L-Methionine (L-Methionine), L-valine, L-cystine, L-arginine (L-arginine, L-arginine), L-isoleucine, L-threonine, L-tryptophan, L-histidine, L-histidine hydrochloride, L-cysteine hydrochloride, amino It is preferably any one or two or more selected from the group consisting of acetic acid (glycine), taurine, a mixture of amino acids and L-leucine.

In addition, as the vitamin-based additives, L-carnitine, niacin (niacin-nicotinic acid), nicotinic acid amide, biotin (vitamin H), vitamin A powder (retinol powder), vitamin A oil (retinol oil), vitamin B1, vitamin B1 Naphthaline-1,5-disulfonate (thiaminnaphthalin-1,5-disulfonate), vitamin B1 lauryl sulfate (thiamine lauryl sulfate), vitamin B1 rhodanate (thiamin thiocyanate), vitamin B1 hydrochloride (Thiamin Hydrochloride), Vitamin B1 Nitrate (Thiamin Nitrate), Vitamin B2 (Riboflavin), Vitamin B2 Sodium Phosphate (Riboflavin 5'-Phosphate Ester Sodium), Vitamin B6 Hydrochloride (Pyridoxine Hydrochloride), Vitamin B12 (Cyanocobalamin), Vitamin C (L-ascorbic acid), Vitamin C calcium salt (L-ascorbic acid calcium salt), Vitamin C-2-phosphate magnesium salt (L-ascorbic acid-2-phosphate ester magnesium), L-ascorbic acid -2-polyethylene glycol ether, vitamin C sodium salt (L-ascorbic acid sodium salt), vitamin D powder (calciferol powder), vitamin D2 (calciferol), vitamin D3 (cholecalciferol), vitamin E (dl -alpha-tocopherol), vitamin E acetate (dl-alpha-tocopherol acetate), d-alpha-vitamin E acetate, vitamin K1 (phyloquinone), vitamin K3 (menadione), vitamin K3 sulfite sodium salt (menadione sulfite) sodium salt), vitamin K3 sulfite nicotinamide (menadione sulfite nicotinamide), vitamin K4 (acetomenaphthone), choline chloride, folic acid, inositol, choline hydrogen tartrate, pantothenic acid and provitamin A (beta-carotene). Any one or two or more selected from the group is preferred, but is not limited thereto.

In addition, the functional additives include coenzyme Q10, bilberry extract, ginseng extract, licorice extract, marigold extract (including lutein and marigold pigment), raspberry extract, wood vinegar, milk thistle extract, artichoke extract, burdock extract, yucca extract, Tea Extract, Natural Betaine (Betaine), Chicory Extract, Arrowroot Extract, Quillaya (Quillaa), Taheebo Extract, Golden Root Extract, Evening Primrose Oil, Grapefruit Seed Extract, Grape Seed Extract, Sunflower Seed Extract, Fenugreek Extract It is preferably any one selected from the group consisting of , sesame oil extract, glucan, mannan, silkworm extract, chitosan, and chitin, but any material known to have human or mammalian functionality may be used.

Meanwhile, the liquid composition of the present invention contains 0.04 to 0.2 parts by weight of lutein, 65 to 75 parts by weight of glycerin, 3 to 5 parts by weight of an emulsifier, 4 to 8 parts by weight of a binder, and 4 to 8 parts by weight of oligosaccharides, based on 100 parts by weight of the liquid composition. Part by weight and preferably 10 to 15 parts by weight of water, lutein 0.04 to 0.3 parts by weight, glycerin 65 to 75 parts by weight, emulsifier 3 to 5 parts by weight, binder 4 to 8 parts by weight based on 100 parts by weight of the liquid composition , 4 to 8 parts by weight of oligosaccharides, 0.1 to 0.3 parts by weight of flavoring, 0.01 to 0.05 parts by weight of vitamin-based additives, 0.05 to 0.2 parts by weight of functional additives, and more preferably 10 to 15 parts by weight of water.

In a specific embodiment of the present invention, the present inventors prepared a liquid feed containing lutein and containing or not containing pullulan (see Tables 1 and 2).

In addition, as a result of evaluating the temperature stability of the prepared liquid feed, the liquid feed composition containing lutein and pullulan was stable in liquid at all temperature conditions of 4 ° C, 25 ° C, 40 ° C, and 45 ° C for 60 days. On the other hand, it was confirmed that the phases of the liquid feed composition containing lutein and not containing pullulan were separated (Table 3, see FIGS. 1 and 2).

Therefore, it was confirmed that the liquid feed composition containing lutein and pullulan of the present invention has stable properties under various temperature conditions and does not cause precipitation, so that it can be usefully used as a health food or feed for pets. did

In addition, the present invention provides a health food or feed composition for improving eye health comprising the liquid composition of the present invention.

The 'feed' of the present invention means nutrition for livestock, animals, fish, etc. including pets, or necessary for health maintenance or growth, and the feed of the present invention may be 'formulated feed' or 'supplemental feed', Not limited.

Specifically, the 'supplementary feed' refers to what is added to the feed to increase the efficacy of the feed, and the 'mixed feed' means that the supplementary feed is mixed or processed in an appropriate ratio to the existing feed, so the feed of the present invention It can be used directly or used as a formulated feed or supplementary feed.

The liquid composition of the present invention may be added to food as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods. The mixing amount of the active ingredient can be suitably determined depending on the purpose of its use (for prevention or improvement). In general, the liquid composition in the health food may be added in an amount of 0.01 to 15% by weight of the total food weight, and the health drink composition may be added in an amount of 0.02 to 5 g, preferably 0.3 to 1 g, based on 100 ml. there is. However, in the case of long-term intake for the purpose of health and hygiene or health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

Hereinafter, the present invention will be described in detail by examples and experimental examples.

However, the following Examples and Experimental Examples specifically illustrate the present invention, and the content of the present invention is not limited by the Examples and Experimental Examples.

<Example 1> Preparation of liquid feed containing lutein

A liquid feed composition containing lutein was prepared according to the composition shown in Table 1 below.

Specifically, glycerin fatty acid ester, glycerin, purified water, lutein, and tocopherol were dissolved by heating to 80°C. Then, after emulsifying using an emulsifier, fructooligosaccharide, pullulan, coenzyme Q10, bilberry extract, and flavoring were added and stirred for 30 minutes. Then, after sterilization at 70 ° C. for 60 minutes, it was stored in a storage container to prepare a liquid feed containing lutein.

ingredient Content (g) manufacturer (country) Glycerin Fatty Acid Esters 41.6 Taejin GNS (Korea) glycerin 713 KLK OLEO (Malaysia) lutein One Allied Biotech (Taiwan) tocopherol 0.2 BASF (Germany) fructooligosaccharides 60 Beksul (Korea) pullulan 53 HAYASHIBARA(Japan) Coenzyme Q10 One Zhejiang Medicine (China) Bilberry Extract 0.1 NATUREX AG (Switzerland) flavoring agent (food flavor) 2 Borak (Korea) Purified water 128.1 self-production

<Example 2> Preparation of liquid feed containing lutein and not containing pullulan

A liquid feed composition containing lutein and not containing pullulan was prepared according to the composition shown in [Table 2] below.

In addition, the method for preparing the liquid feed composition was prepared in the same manner as in <Example 1>.

ingredient Content (g) Glycerin Fatty Acid Esters 41.6 glycerin 713 lutein One tocopherol 0.2 fructooligosaccharides 60 pullulan - Coenzyme Q10 One Bilberry Extract 0.1 flavoring agent (food flavor) 2 Purified water 181.1

<Experimental Example 1> Evaluation of temperature stability

The temperature stability of the liquid feed composition containing lutein prepared in <Example 1> and <Example 2> and containing or not containing pullulan was evaluated.

Specifically, the compositions prepared in <Example 1> and <Example 2> were placed in an incubator at 4° C., 25° C., 40° C., and 45° C. for each temperature, and the properties, color, separation, and precipitation for 60 days at weekly intervals. phenomenon was observed.

As a result, as shown in Table 3, FIGS. 1 and 2, the liquid feed composition containing lutein and pullulan is liquid stable at all temperature conditions of 4 ° C, 25 ° C, 40 ° C, and 45 ° C for 60 days. On the other hand, it was confirmed that the phases of the liquid feed composition containing lutein and not containing pullulan were separated (Table 3, Figs. 1 and 2).

Therefore, it was confirmed that the liquid feed composition containing lutein and pullulan of the present invention has stable properties under various temperature conditions and does not cause precipitation, so that it can be usefully used as a health food or feed for pets. did

division temperature condition Composition of <Example 1> Composition of <Example 2>
1 day elapsed 4℃ stability stability 25℃ stability stability 40℃ stability stability 45℃ stability stability
7 days elapsed 4℃ stability stability 25℃ stability stability 40℃ stability phase separation 45℃ stability sex separation
14 days elapsed 4℃ stability stability 25℃ stability phase separation 40℃ stability phase separation 45℃ stability phase separation
21 days passed 4℃ stability stability 25℃ stability phase separation 40℃ stability phase separation 45℃ stability phase separation
28 days passed 4℃ stability stability 25℃ stability phase separation 40℃ stability phase separation 45℃ stability phase separation
35 days passed 4℃ stability stability 25℃ stability phase separation 40℃ stability phase separation 45℃ stability phase separation
42 days elapsed 4℃ stability stability 25℃ stability phase separation 40℃ stability phase separation 45℃ stability phase separation
60 days 4℃ stability stability 25℃ stability phase separation 40℃ stability phase separation 45℃ stability phase separation

Claims (13)

A liquid composition containing lutein, glycerin, an emulsifier, a binder, an oligosaccharide, and water as active ingredients.
The liquid composition according to claim 1, further comprising at least one selected from the group consisting of flavors, amino acid additives, vitamin additives and functional additives.
The method of claim 1, wherein the emulsifier is glycerin fatty acid ester, lecithin, sodium metaphosphate, sorbitan fatty acid ester, sodium stearyl lactate, calcium stearyl lactate, sucrose fatty acid ester, polyoxyethylene glycerol fatty acid ester, polyoxyethylene sorbitan A liquid composition, characterized in that any one or two or more selected from the group consisting of fatty acid esters, sodium polyphosphate and sodium stearyl lactate.
The liquid composition according to claim 1, wherein the binder is any one selected from the group consisting of natural binders, synthetic binders, and mixtures.
The method of claim 4, wherein the natural binder is any one selected from the group consisting of pullulan, guar gum, lignin sulfonate (lignin sulfonate), cellulose, rosin, gelatin, natural gum, casein, and collagen. Liquid composition to be.
The method of claim 4, wherein the synthetic binder is selected from the group consisting of sodium alginate, ammonium alginate, potassium alginate, sodium carboxymethyl cellulose, sodium caseinate, polymethyl carbamide, sodium polyacrylate and hydroxypropylmethyl cellulose. A liquid composition, characterized in that any one.
The liquid composition according to claim 4, wherein the mixture is any one selected from the group consisting of a natural binder mixture, a synthetic binder mixture, and a binder mixture.
The method of claim 1, wherein the oligosaccharide is any one selected from the group consisting of galactooligosaccharide, gentioligosaccharide, maltooligosaccharide, isomaltooligosaccharide, xylooligosaccharide, chitooligosaccharide, fructooligosaccharide and oligosaccharide mixture. A liquid composition for use.
The method of claim 1, based on 100 parts by weight of the liquid composition, lutein 0.04 to 0.3 parts by weight, glycerin 65 to 75 parts by weight, emulsifier 3 to 5 parts by weight, binder 4 to 8 parts by weight, oligosaccharide 4 to 8 parts by weight and 10 to 15 parts by weight of water.
The method of claim 1, based on 100 parts by weight of the liquid composition, lutein 0.04 to 0.3 parts by weight, glycerin 65 to 75 parts by weight, emulsifier 3 to 5 parts by weight, binder 4 to 8 parts by weight, oligosaccharide 4 to 8 parts by weight , 0.1 to 0.3 parts by weight of flavor, 0.01 to 0.05 parts by weight of vitamin-based additives, 0.05 to 0.2 parts by weight of functional additives, and 10 to 15 parts by weight of water.
A health food for improving eye health containing the liquid composition of claim 1 as an active ingredient.
A feed composition containing the liquid composition of claim 1 as an active ingredient.
[Claim 13] The feed composition according to claim 12, wherein the feed is a supplementary feed or formulated feed.

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