KR20230059914A - Mass propagation method of Dysophylla yatabeana Makino - Google Patents

Abstract

As one aspect, the present invention provides a method for mass propagation of Dysophylla yatabeana Makino, including a step of irradiating an artificial light source with a frequency of 4,000 to 5,000 Hz. The present invention has an advantage of being useful for individual conservation by allowing mass propagation of Dysophylla yatabeana Makino, an endangered species, by cultivating Dysophylla yatabeana Makino under specific conditions.

Description

Mass propagation method of Jeonju water tail grass {Mass propagation method of Dysophylla yatabeana Makino}

The present invention relates to a mass propagation method of Jeonju japonica, and specifically, to a cultivation method capable of mass-producing Jeonju japonica by irradiating an artificial light source of a specific frequency.

Smart farms, which can stably produce crops in a restricted space due to various extreme weather events caused by recent frequent climate change, can produce crops efficiently regardless of external environmental factors. LED used as an artificial light source for plant cultivation in smart farms is easy to install in a small space, has a long lifespan, and can be irradiated intermittently in minutes or seconds, so it is economical with less power consumption than other light sources. In addition, it is possible to select the light quality of a specific wavelength, so that functions such as promoting photosynthesis and controlling flowering can be performed. In order to produce high value-added crops with the smart farm production method, it is necessary to apply various additional environmental stresses that can increase the quality and content of functional components in an appropriate cultivation system that can secure productivity. Among these stresses, light environment control is the most effective way to increase production.

Conventionally, spinach is cultivated in an enclosed plant factory system that promotes the growth of spinach by adjusting the light intensity and photoperiod of light of an LED lamp wavelength in which red: blue: white is 8:1:1. There is a literature on the method (Korean Patent Publication No. 10-2017-0025460).

In addition, there is a document (Korean Patent Registration No. 10-1439009) on a method of cultivating sagebrush in a closed plant factory system through control of EC, light intensity, planting distance, etc. of nutrient solution.

However, concepts such as frequency (Hz) and duty ratio are not included in light qualities such as light intensity and photoperiod disclosed in the above-mentioned literature.

Since duty ratio affects power saving, research on plant response is being conducted, but research on plant growth according to frequency (Hz) is lacking.

Korean Registered Patent No. 10-1439009 (registered on September 1, 2014) Korean Patent Publication No. 10-2017-0025460 (published on March 8, 2017)

The problem to be solved by the present invention is to provide a method for mass propagation of Dysophylla yatabeana Makino, which includes irradiating an artificial light source at a frequency of 50 to 5,000 Hz.

The present invention, in one aspect, provides a method for mass propagation of Dysophylla yatabeana Makino, comprising the step of irradiating an artificial light source at a frequency of 4,000 to 5,000 Hz.

In the artificial light source, red light of 620 to 700 nm and blue light of 430 to 480 nm may be mixed at a light quantity ratio of 10 to 12:5 to 8.

A duty ratio of the artificial light source may be 50 to 70%.

The luminous intensity of the artificial light source is 93.67 to 107.23 μmol/m ² s, and the photoperiod may be 14 to 18 hours.

The cultivation temperature of the jeonju water tail grass is 20 ~ 25 ℃, the relative humidity may be 50 ~ 85%.

The present invention provides a jeonju water tail grass grown by the above method in another aspect.

The present invention has an advantage in that it is possible to mass-proliferate Jeonju watertail, which is an endangered species, by cultivating Jeonju watertail under specific conditions, which is useful for object preservation.

Hereinafter, a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings. First of all, it should be noted that in the drawings, the same components or parts are denoted by the same reference numerals as much as possible. In describing the present invention, detailed descriptions of related well-known functions or configurations are omitted to avoid obscuring the subject matter of the present invention.

As used herein, the terms about, substantially, etc. are used at or close to the value when manufacturing and material tolerances inherent in the referenced meaning are given, and are intended to be accurate or Absolute numbers are used to prevent exploitation by unscrupulous infringers of the stated disclosure.

In order to achieve the above purpose, the growth response of Jeonju japonica grown by irradiating artificial light sources with different duty ratios and frequencies was measured, and Jeonju plants grown at 4,000 to 5,000 Hz when the duty ratio was 50 to 70%. The present invention was completed by confirming that the growth response of the tail grass was better than that of the long tail grass grown in other frequency bands.

Prior to the description of the present invention, terms used in the present invention will be briefly described.

In the present invention, the term "smart farm" refers to a system that produces agricultural products throughout the year in a facility, such as producing industrial products using advanced technologies such as environmental control and automation, completely blocked from the outside and crops only with artificial lighting It is divided into a completely controlled type that grows crops and a solar-powered type that grows crops using both sunlight and artificial lighting indoors, such as a greenhouse. In other words, smart farm is a technology that can optimally control environmental conditions such as temperature, light, CO ₂ , and culture medium that affect crop growth and automate work processes to produce crops within the facility regardless of weather conditions. it means.

In the present invention, the term " Dysophylla yatabeana Makino " is a plant belonging to the genus Lamiaceae ( Labiatae ), and lives in a wetland formed in a lowland grass field of about 130 m above sea level in Jeju Island. Currently, Jeonju watertail grass is evaluated as an endangered species (EN) due to reclamation of wetlands and environmental pollution. . The Jeonju water tail grass can reproduce asexually through the underground stem, and the growth rate of the underground stem is an important factor in mass propagation of the object. Therefore, it is important to understand the growth reaction of underground rhizomes and to set appropriate light conditions.

In the present invention, the term "artificial light source" refers to an artificial device that supplies light so that plants can photosynthesize.

In the present invention, the term "irradiation" means to shine light on the surface, and exposes the light of an artificial light source to the surface of the plant.

In the present invention, the term "light intensity" means photosynthetic photon flux density (PPFD), and means the amount of photons falling per unit time per unit area.

In the present invention, the term "duty ratio" is a term used in a pulse having a period and means an on duty ratio. This refers to the ratio of time that the pulse is on for one period. Therefore, if the duty ratio is lowered, the power consumption can be reduced because the off state is prolonged.

In the present invention, “memory” means the time to irradiate an artificial light source (light) in a plant factory (smart farm), and memorization means the time to block light in a plant factory. The photoperiod means that the memorization and the memorization are repeated at a constant time, and the memorization is the time excluding the memorization based on 24 hours (one day).

The present invention, in one aspect, provides a mass propagation method of Jeonjumul tail grass ( Dysophylla yatabeana Makino) comprising the step of irradiating an artificial light source at a frequency of 4,000 to 5,000 Hz.

The jeonju water tail grass can be grown in a smart farm, and the smart farm can control the wavelength band, frequency, luminous intensity, duty ratio, photoperiod, temperature and humidity conditions of the irradiated light. It can be a fully controlled smart farm with

The frequency of the artificial light source may be 50 to 5,000 Hz, and if the frequency is less than 50 Hz, the reduction rate of chlorophyll (chlorophyll) P700 centered on photosystem I of plants is about 20ms (50hz). There is a problem of falling, and when it exceeds 5,000 Hz, considering that the reduction rate of Chlorophyll (chlorophyll) P680 centered in the photosystem II of plants is about 200 μs (5000 Hz), there is a problem of lowering photosynthesis and power efficiency, as described above. Range is preferred.

In this case, the artificial light source may be one selected from the group consisting of a resistance light bulb, a discharge lamp, and a light-emitting diode (LED), and specifically may be a light-emitting diode (LED). The LED may be at least one selected from the group consisting of blue light, red light, yellow light, far red light, and white light, and specifically, may be a mixed light of blue light and red light.

The artificial light source may be a mixture of red light of 620 to 700 nm and blue light of 430 to 480 nm at a ratio of 10 to 12:5 to 8, specifically, red light of 620 to 670 nm and blue light of 430 to 450 nm. It may be a mixed light of blue light, and more specifically, it may be a mixed light of red light of 630 to 660 nm and blue light of 440 nm. In addition, the light quantity ratio of the red light and the blue light may be 10 to 12:5 to 8, specifically, it may be 11:7.

The duty ratio of the artificial light source may be 50 to 70%, and if the duty ratio is less than 50%, the power consumption is low, reducing cultivation costs, but there is a problem in the growth of jeonju watertail.

The luminous intensity of the artificial light source is 93.67 to 107.23 μmol/m ² s, and the photoperiod may be 14 to 18 hours. Specifically, the light intensity may be 100.46 μmol/m ² s, the photoperiod may be 16 hours, and the dark period may be 8 hours. That is, in the smart farm, the artificial light source is irradiated with light at a light intensity of 93.67 to 107.23 μmol / m ^s for 16 hours (specialization) and blocking the light for 8 hours (memorization). can be cultivated.

If the light intensity of the artificial light source is less than 93.67 μmol / m ² s, there is a problem that the growth of the plant does not occur, and if it exceeds 107.23 μmol / m ² s, the growth of the plant is promoted, but the electricity consumption increases and the cultivation cost increases There is a problem of doing, the above range is preferable.

In addition, if the light cycle is less than 14 hours, as the light intensity decreases, there is a problem with growth and a decrease in production yield due to a decrease in photosynthetic action, and when the light cycle exceeds 18 hours, the light saturation is achieved and the photosynthetic efficiency is increased. Rather, there is a problem of decreasing and eventually inhibiting growth, and the above range is preferable.

The Jeonju watertail cultivation temperature may be 20 ~ 25 ℃ and the relative humidity may be 50 ~ 85%, specifically, the cultivation temperature may be 22 ~ 24 ℃, the relative humidity may be 75 ~ 85%.

When the cultivation temperature is less than 20 ° C and the relative humidity is less than 50%, considering the environment of the native place (original habitat) of Jeonju watertail grass (living in a sunny plant wetland), there is a problem in supplying moisture due to low humidity, and the cultivation temperature is When the temperature exceeds 25 ° C and the relative humidity exceeds 85%, the leaves and stems of plants are burned or aged due to high temperature stress, and productivity and quality are deteriorated, such as inhibition of shoot and root growth. Range is preferred.

In another aspect, the present invention provides a Jeonju Trifolium cultivated by the cultivation method of the Jeonju Tripod.

Hereinafter, the present invention will be described in detail according to examples. However, the following examples are only to illustrate the present invention, and the content of the present invention is not limited to the following examples.

<Example 1>

After putting the top soil in white plastic and transplanting the Jeonju watertail grass, the Jeonju watertail grass was grown by irradiating the LED light source in the smart farm.

At this time, the LED light source is a mixed light source in which red light of 630 to 660 nm and blue light of 440 nm are mixed in a light quantity ratio of 11:7.

Cultivation conditions during the cultivation period are shown in Table 1 below.

[Table 1]

<Comparative Examples 1 and 2>

Cultivated in the same manner as in Example 1, but the cultivation conditions during the cultivation period are shown in Table 2 below.

[Table 2]

<Comparative Example 3>

Cultivation was performed in the same manner as in Example 1, but a mixed light source in which red light of 630 to 660 nm, blue light of 440 nm, and white light of 450 to 540 nm was mixed at a ratio of 11:4:3 was used.

<Comparative Example 4>

Cultivation was performed in the same manner as in Comparative Example 1, but a mixed light source in which red light of 630 to 660 nm, blue light of 440 nm, and white light of 450 to 540 nm was mixed at a ratio of 11:4:3 was used.

<Comparative Example 5>

Cultivation was performed in the same manner as in Comparative Example 2, but a mixed light source in which red light of 630 to 660 nm, blue light of 440 nm, and white light of 450 to 540 nm was mixed at a ratio of 11:4:3 was used.

<Experimental example>

In order to confirm the growth reaction of Jeonju watertail plant grown according to Example 1 and Comparative Examples 1 to 5, the length of the underground stem, the weight of the underground stem, the length of the above-ground part, the weight of the above-ground part and the number of leaves were measured.

Table 3 below shows the growth reactions of Example 1 and Comparative Examples 1 to 5 according to changes in Duty and H ₂ .

[Table 3]

Referring to Table 3, comparing the growth of the cultivated Jeonju japonica, first, in the case of the length of the underground stem, Comparative Example 1 was 19.55 ± 9.03 cm, Comparative Example 2 was 10.9 ± 4.45 cm, Comparative Example 3 was 18.1 ± 8.1 cm, Comparative Example 3 Example 4 measured 17.2 ± 7.96 cm and Comparative Example 5 measured 19.0 ± 8.07 cm, while Example 1 measured 19.8 ± 9.12 cm, it can be seen that the longest. In addition, in the case of the length of the aerial part, Comparative Example 1 was 38.7 ± 6.5 cm, Comparative Example 2 was 39.7 ± 7.3 cm, Comparative Example 3 was 38.1 ± 4.7 cm, Comparative Example 4 was 34.4 ± 4.6 cm, and Comparative Example 5 was 23.1 ± 4.3 cm. In contrast to the measured length of Example 1, it can be seen that the length of the underground diameter was measured as 40.8 ± 5.6 cm, which is the longest.

In addition, in the case of the number of leaves of the cultivated Jeonju japonica, Comparative Example 1 was 38 ± 5.5 ea, Comparative Example 2 was 35.7 ± 11.2 ea, Comparative Example 3 was 27.8 ± 3.9 ea, Comparative Example 4 was 30.6 ± 4.2 ea and Comparative Example 5 was measured at 28.6 ± 6.4 ea, whereas Example 1 was measured at 41.4 ± 6.7 ea, confirming the highest number of leaf waters.

That is, when the duty ratio is 50% and the light amount ratio of the mixed light of red and blue light at 5000Hz (Example 1) is 11:7, it can be seen that the effect of growth (length of underground stem, length of above-ground part and number of leaves) is the best. In addition, in the case of mixed light, as can be seen in Comparative Examples 3 to 5 in which white light is further mixed, it can be seen that the mixed light of red and blue is more suitable for the growth of japonica than the mixed light of red, blue and white, and the ratio When the ratio was 11:7, the effect was most excellent.

The above examples are only for exemplifying the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples. Therefore, the disclosed embodiments should be considered from an illustrative rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent scope will be construed as being included in the present invention.

Claims (6)

Including irradiating an artificial light source with a frequency of 4,000 to 5,000 Hz,
Method for mass propagation of Dysophylla yatabeana Makino. According to claim 1,
The artificial light source,
Characterized in that the red light of 620 ~ 700 nm and the blue light of 430 ~ 480 nm are mixed at a light quantity ratio of 10 ~ 12: 5 ~ 8,
Mass propagation method of Jeonju watertail grass. According to claim 1,
The duty ratio of the artificial light source is
Characterized in that 50 to 70%,
A method for mass propagation of Jeonju watertail grass. According to claim 1,
The luminous intensity of the artificial light source,
93.67 ~ 107.23 μmol / m ² s, characterized in that the photoperiod is 14 ~ 18 hours,
Mass propagation method of Jeonju watertail grass. According to claim 1,
Characterized in that the cultivation temperature of the Jeonju watertail grass is 20 to 25 ℃, and the relative humidity is 50 to 85%.
Mass propagation method of Jeonju watertail grass. Claims 1 to 5 of the method of any one of the cultivated jeonju water tail grass.