KR20230031281A - 바이러스 정제를 위한 재료 및 방법 - Google Patents

바이러스 정제를 위한 재료 및 방법 Download PDF

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KR20230031281A
KR20230031281A KR1020237000108A KR20237000108A KR20230031281A KR 20230031281 A KR20230031281 A KR 20230031281A KR 1020237000108 A KR1020237000108 A KR 1020237000108A KR 20237000108 A KR20237000108 A KR 20237000108A KR 20230031281 A KR20230031281 A KR 20230031281A
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aav particles
mnase
buffer
purified
fold
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KR1020237000108A
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Korean (ko)
Inventor
브라이언 이. 탐커비치
메튜 피. 에르콜리노
스테펀 티. 스파놀
사캬 싱 모하파트라
윌리엄 리로이드 3세 페리
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얀센 바이오테크 인코포레이티드
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Publication of KR20230031281A publication Critical patent/KR20230031281A/ko
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    • CCHEMISTRY; METALLURGY
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14123Virus like particles [VLP]
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    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151Methods of production or purification of viral material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/31Endoribonucleases active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters (3.1.31)
    • C12Y301/31001Micrococcal nuclease (3.1.31.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020237000108A 2020-06-02 2021-06-01 바이러스 정제를 위한 재료 및 방법 Withdrawn KR20230031281A (ko)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US202063033631P 2020-06-02 2020-06-02
US202063033549P 2020-06-02 2020-06-02
US202063033492P 2020-06-02 2020-06-02
US202063033731P 2020-06-02 2020-06-02
US202063033643P 2020-06-02 2020-06-02
US202063033531P 2020-06-02 2020-06-02
US202063033449P 2020-06-02 2020-06-02
US63/033,731 2020-06-02
US63/033,449 2020-06-02
US63/033,643 2020-06-02
US63/033,492 2020-06-02
US63/033,631 2020-06-02
US63/033,531 2020-06-02
US63/033,549 2020-06-02
PCT/US2021/035113 WO2021247476A1 (en) 2020-06-02 2021-06-01 Materials and methods for viral purification

Publications (1)

Publication Number Publication Date
KR20230031281A true KR20230031281A (ko) 2023-03-07

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KR1020237000108A Withdrawn KR20230031281A (ko) 2020-06-02 2021-06-01 바이러스 정제를 위한 재료 및 방법

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Country Link
US (1) US20230212528A1 (https=)
EP (1) EP4157301A4 (https=)
JP (1) JP2023528406A (https=)
KR (1) KR20230031281A (https=)
CN (1) CN116096867A (https=)
AU (1) AU2021284250A1 (https=)
CA (1) CA3185729A1 (https=)
WO (1) WO2021247476A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120092093A (zh) * 2022-08-25 2025-06-03 富士胶片株式会社 靶病毒的制造方法及培养组合物
KR20260019635A (ko) * 2023-06-09 2026-02-10 사르토리우스 비아이에이 세퍼레이션스 디.오.오. 완전 충전 아데노 관련 바이러스 (aav) 캡시드의 향상된 분리 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001522244A (ja) * 1997-04-24 2001-11-13 ユニバーシティ オブ ワシントン パルボウイルスベクターによる標的化した遺伝子改変
US6156303A (en) * 1997-06-11 2000-12-05 University Of Washington Adeno-associated virus (AAV) isolates and AAV vectors derived therefrom
AU4671300A (en) * 1999-04-30 2000-11-17 City Of Hope The use of adeno-associated virus (aav) deliver cytoprotective genes
BRPI0511764B8 (pt) * 2004-06-01 2021-05-25 Avigen Inc método de prevenção de agregação de vírions de vírus adeno-associado recombinante (raav) em uma preparação purificada de virions raav
ES2934848T3 (es) * 2015-12-11 2023-02-27 Univ Pennsylvania Método de purificación escalable para AAV8
US11028372B2 (en) * 2015-12-11 2021-06-08 The Trustees Of The University Of Pennsylvania Scalable purification method for AAVRH10
UY37620A (es) * 2017-02-28 2018-08-31 Univ Pennsylvania Vacunas contra la gripe mediadas por aav novedosas
CA3190596A1 (en) * 2020-08-07 2022-02-10 Janssen Biotech, Inc. Formulations for highly purified viral particles

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Publication number Publication date
US20230212528A1 (en) 2023-07-06
WO2021247476A1 (en) 2021-12-09
AU2021284250A1 (en) 2023-02-09
EP4157301A1 (en) 2023-04-05
CN116096867A (zh) 2023-05-09
JP2023528406A (ja) 2023-07-04
EP4157301A4 (en) 2025-03-19
CA3185729A1 (en) 2021-12-09

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