CA3185729A1 - Materials and methods for viral purification - Google Patents
Materials and methods for viral purificationInfo
- Publication number
- CA3185729A1 CA3185729A1 CA3185729A CA3185729A CA3185729A1 CA 3185729 A1 CA3185729 A1 CA 3185729A1 CA 3185729 A CA3185729 A CA 3185729A CA 3185729 A CA3185729 A CA 3185729A CA 3185729 A1 CA3185729 A1 CA 3185729A1
- Authority
- CA
- Canada
- Prior art keywords
- mnase
- aav particles
- buffer
- viral titer
- fold
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14123—Virus like particles [VLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/31—Endoribonucleases active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters (3.1.31)
- C12Y301/31001—Micrococcal nuclease (3.1.31.1)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (15)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063033631P | 2020-06-02 | 2020-06-02 | |
| US202063033549P | 2020-06-02 | 2020-06-02 | |
| US202063033492P | 2020-06-02 | 2020-06-02 | |
| US202063033731P | 2020-06-02 | 2020-06-02 | |
| US202063033643P | 2020-06-02 | 2020-06-02 | |
| US202063033531P | 2020-06-02 | 2020-06-02 | |
| US202063033449P | 2020-06-02 | 2020-06-02 | |
| US63/033,731 | 2020-06-02 | ||
| US63/033,449 | 2020-06-02 | ||
| US63/033,643 | 2020-06-02 | ||
| US63/033,492 | 2020-06-02 | ||
| US63/033,631 | 2020-06-02 | ||
| US63/033,531 | 2020-06-02 | ||
| US63/033,549 | 2020-06-02 | ||
| PCT/US2021/035113 WO2021247476A1 (en) | 2020-06-02 | 2021-06-01 | Materials and methods for viral purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3185729A1 true CA3185729A1 (en) | 2021-12-09 |
Family
ID=78829875
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3185729A Pending CA3185729A1 (en) | 2020-06-02 | 2021-06-01 | Materials and methods for viral purification |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20230212528A1 (https=) |
| EP (1) | EP4157301A4 (https=) |
| JP (1) | JP2023528406A (https=) |
| KR (1) | KR20230031281A (https=) |
| CN (1) | CN116096867A (https=) |
| AU (1) | AU2021284250A1 (https=) |
| CA (1) | CA3185729A1 (https=) |
| WO (1) | WO2021247476A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120092093A (zh) * | 2022-08-25 | 2025-06-03 | 富士胶片株式会社 | 靶病毒的制造方法及培养组合物 |
| KR20260019635A (ko) * | 2023-06-09 | 2026-02-10 | 사르토리우스 비아이에이 세퍼레이션스 디.오.오. | 완전 충전 아데노 관련 바이러스 (aav) 캡시드의 향상된 분리 방법 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001522244A (ja) * | 1997-04-24 | 2001-11-13 | ユニバーシティ オブ ワシントン | パルボウイルスベクターによる標的化した遺伝子改変 |
| US6156303A (en) * | 1997-06-11 | 2000-12-05 | University Of Washington | Adeno-associated virus (AAV) isolates and AAV vectors derived therefrom |
| AU4671300A (en) * | 1999-04-30 | 2000-11-17 | City Of Hope | The use of adeno-associated virus (aav) deliver cytoprotective genes |
| BRPI0511764B8 (pt) * | 2004-06-01 | 2021-05-25 | Avigen Inc | método de prevenção de agregação de vírions de vírus adeno-associado recombinante (raav) em uma preparação purificada de virions raav |
| ES2934848T3 (es) * | 2015-12-11 | 2023-02-27 | Univ Pennsylvania | Método de purificación escalable para AAV8 |
| US11028372B2 (en) * | 2015-12-11 | 2021-06-08 | The Trustees Of The University Of Pennsylvania | Scalable purification method for AAVRH10 |
| UY37620A (es) * | 2017-02-28 | 2018-08-31 | Univ Pennsylvania | Vacunas contra la gripe mediadas por aav novedosas |
| CA3190596A1 (en) * | 2020-08-07 | 2022-02-10 | Janssen Biotech, Inc. | Formulations for highly purified viral particles |
-
2021
- 2021-06-01 CN CN202180050117.9A patent/CN116096867A/zh active Pending
- 2021-06-01 AU AU2021284250A patent/AU2021284250A1/en not_active Abandoned
- 2021-06-01 EP EP21818554.4A patent/EP4157301A4/en active Pending
- 2021-06-01 US US18/000,525 patent/US20230212528A1/en active Pending
- 2021-06-01 KR KR1020237000108A patent/KR20230031281A/ko not_active Withdrawn
- 2021-06-01 JP JP2022573726A patent/JP2023528406A/ja active Pending
- 2021-06-01 WO PCT/US2021/035113 patent/WO2021247476A1/en not_active Ceased
- 2021-06-01 CA CA3185729A patent/CA3185729A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR20230031281A (ko) | 2023-03-07 |
| US20230212528A1 (en) | 2023-07-06 |
| WO2021247476A1 (en) | 2021-12-09 |
| AU2021284250A1 (en) | 2023-02-09 |
| EP4157301A1 (en) | 2023-04-05 |
| CN116096867A (zh) | 2023-05-09 |
| JP2023528406A (ja) | 2023-07-04 |
| EP4157301A4 (en) | 2025-03-19 |
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