KR20230010321A - System for measuring organic matter caused by micro-organisms using fluorescence spectroscopy of continuous type, and method for the same - Google Patents

Abstract

The present invention provides a measuring system for an organic matter caused by microorganisms using continuous fluorescence spectroscopy and a method thereof, which can characterize and quantify an organic matter caused by microorganisms which can be used as a biological index and monitor water quality by using and measuring the inherent fluorescence of tryptophan which is an amino acid associated with a microorganism activity which is an organic matter caused by underwater microorganisms by using continuous fluorescence spectroscopy to correspond to continuously supplied samples. Also, the population of colon bacillus can be calculated by using the fluorescent intensity of samples by using an excitation wavelength in a range of 270-290nm and an emission wavelength of 300nm or 350nm for tryptophan measurement. Also, the fluorescent intensity of tryptophan can be measured to calculate BOD5. The contamination of microorganisms can be indirectly monitored. Alarm information in accordance with a microorganism warning range can be generated and transmitted.

Description

Microorganism-derived organic matter measurement system and method using a continuous fluorescence spectrometer

The present invention relates to a system for measuring organic matter originating from microorganisms, and more particularly, to measure organic matter originating from microorganisms in water using a continuous fluorescence spectroscopy device, thereby characterizing and quantifying organic matter originating from microorganisms, continuous fluorescence analysis. It relates to a system for measuring organic matter caused by microorganisms using a device and a method therefor.

In general, methods for real-time analysis of natural organic matter present in water include a UV254 absorbance measurement method and a total organic carbon (TOC) measurement method. These UV254 absorbance measurement methods and TOC measurement methods are It is the most comprehensive and widely adopted method used to quantify the presence of organic carbon atoms in

Specifically, in natural water that exists in water, there are proteins derived from microorganisms and dissolved organic matter caused by organic matter. The UV254 absorbance measurement method and the total organic carbon measurement method are suitable for measuring dissolved organic matter caused by microorganisms (tryptophan). -like; There is a limitation that it is not suitable for measuring tryptophan). Microorganism-derived organic matter (tryptophan) is a value that reflects the fluorescence properties of protein compounds and is used as an indicator of biological activity in water.

In addition, microorganisms present in water are composed of a wide variety of pathogens, and these pathogens may vary depending on the environment. Accordingly, it is practically not possible to regularly isolate or identify each specific pathogen present in the water. Accordingly, microbiological measurement methods according to the prior art have only relied on population counts, for example, relying on detection of populations of general bacteria and Escherichia coli for contamination.

On the other hand, the evaluation of microbial contamination present in water is ultimately related to the presence of pathogens. Methods using general bacteria and E. It is time-consuming and the measurement process is cumbersome. In particular, even if the measurement results are obtained after a predetermined time, it is difficult to quickly respond to potential contamination accidents because the contamination of microorganisms has already progressed significantly.

As a method for monitoring microbial water quality according to the prior art, there is a method using Adenosine Tri-Phosphate (ATP), but most of the microbial water quality monitoring equipment for this is expensive, and the reagents used are expensive, and these reagents are refrigerated. Since storage is essential, there is a problem in that there is a risk of reagent deterioration.

In addition, the biochemical oxygen demand (BOD) measurement method is a method of indirectly measuring the amount of dissolved oxygen used in the process of decomposing microorganisms present in water as the amount of organic matter. However, this BOD measurement method takes 5 days or more of analysis time, and in particular, since it is a laboratory-based measurement method, there is a problem in that a rapid response to potential contamination accidents is not suitable.

On the other hand, as a prior art related to the biochemical oxygen demand (BOD) described above, Korean Patent Registration No. 10-1305993 discloses an invention entitled "Method for Measuring BOD Using Qualitative and Quantitative Analysis of Organic Matter", which is shown in FIG. Refer to and explain.

1 is an operational flow chart showing a BOD measurement method using qualitative and quantitative analysis of organic matter according to the prior art.

Referring to FIG. 1 , in the BOD measurement method using qualitative and quantitative analysis of organic matter according to the prior art, first, a statistical analysis of organic matter contained in water quality is performed (S10). Here, the statistical analysis of organic matter may be statistical analysis of dissolved organic carbon (Biochemical Oxygen Demand: DOC), conductivity, fluorescence characteristics, and ultraviolet absorbance.

Next, a correlation equation is extracted based on qualitative analysis and quantitative analysis (S20), and then the extracted correlation equation is stored (S30).

Next, parameters including dissolved organic carbon (DOC), conductivity, fluorescence characteristics, and ultraviolet absorbance of the water quality to be measured are measured (S40).

Next, BOD is determined by substituting the measured variables into the correlation equation (S50). Here, the biochemical oxygen demand (BOD) means the amount of oxygen required when microorganisms that breathe oxygen ingest organic matter for 5 days.

According to the BOD measurement method using qualitative and quantitative analysis of organic substances according to the prior art, BOD of water quality can be measured through quantitative and qualitative analysis of organic substances, but as described above, it takes more than 5 days for analysis. There is a problem.

On the other hand, as a related technology, Korean Patent Registration No. 10-1735464 discloses an invention titled "Apparatus and method for measuring the existence of organic matter or life and death of living things", which will be described with reference to FIG. 2 .

2 is a configuration diagram of a life and death measuring device for measuring the existence of organic matter or the life and death of living things according to the prior art.

Referring to FIG. 2 , a life and death measuring device 10 for measuring the existence of organic matter or the life and death of living things according to the prior art is a device for measuring the existence of organic matter or the life and death of living things through infrared absorption and absorption analysis, and is an infrared light source. (11), a sample container 12, a monochromator 13, an infrared detection unit 14, a determination unit 15, a recording unit 16, a determination unit 17, and a scanning motor 18.

The infrared light source 11 irradiates the sample contained in the sample container 12 with infrared rays 19 for spectroscopic analysis, and the infrared detector 14 detects infrared rays transmitted or reflected from the sample.

The determination unit 15 identifies an amide infrared absorption peak of the sample using the detected infrared rays, and determines the existence of an organic substance in the sample or the life or death of an organism from the identified amide infrared absorption peak. That is, the presence or absence of an amide infrared absorption peak determines the existence of an organic substance in a sample, and the life or death of an organism in a sample can be determined by a shift in the wave number of the amide infrared absorption peak. In this case, the amide infrared absorption peak may be at least one of the infrared absorption peaks of amide I, amide II, and amide III of the protein.

The monochromator 13 disperses light emitted from the infrared light source 11 into a limited range of wavelengths, and selectively allows only radiation of a specific wavelength to reach the infrared detector 14 .

The recording unit 16 measures and records the transmittance obtained from the infrared detection unit 14, and the display unit 17 displays the infrared detection spectrum.

The scan motor 18 is connected to the monochromator 13 and the recording unit 16 and matches them according to the change in wavelength.

According to the prior art life and death measuring device for measuring the existence of organic matter or the life and death of living things, the measurement is simple and quantifiable without the use of reagents through a simple measurement process using infrared absorption spectroscopy, and the same The presence or absence of cells or tissues in the sample and the process of life and death change can be continuously measured.

However, in the case of a life and death measuring device for measuring the presence or death of organic matter according to the prior art, it is a method of determining the presence of organic matter in a biological sample by the presence or absence of an amide infrared absorption peak, so it is not suitable for measuring organic matter in water There is a limit that no

On the other hand, as another related technology, Korean Patent Registration No. 10-1865587 discloses an invention titled "apparatus and method for monitoring microbial harmfulness in real time in water quality", which will be described with reference to FIG. 3 .

Figure 3 is a block diagram of a real-time monitoring device for microbial harmfulness in water quality according to the prior art.

Referring to FIG. 3 , a real-time monitoring device for microbial harmfulness in water quality according to the prior art includes a first measurement module 20, a second measurement module 30, and an analysis module 40, and the analysis module 40 ) includes a multiple regression storage DB 41, a calculation processing unit 42, a reference value excess determination unit 43, and a transmission unit 44.

The first measurement module 20 measures the number of specific microbial colonies formed after culturing specific microorganisms in the vagina in a culture medium.

The second measurement module 30 measures the community distribution characteristics of all microorganisms in the water quality by flow cytometry (FCM).

The analysis module 40 receives measurement values from the first measurement module 20 and the second measurement module 30 and estimates the quantitative amount of a specific microorganism in the water quality in real time using a statistical analysis technique. Specifically, the calculation process unit 42 of the analysis module 40 calculates the quantitative amount of a specific microorganism in the water quality using a multiple regression equation stored in the multiple regression equation storage database 41, and furthermore, the analysis module 40 ) of the standard value determination unit 43 determines whether the quantitative amount of the estimated specific microorganism exceeds a predetermined harmfulness standard value, and also, the transmitter 44 of the analysis module 40 exceeds the harmfulness standard value, wireless It performs a function of transmitting at least one of voice, image, and text information to a predetermined terminal through communication.

According to the real-time monitoring device for microbial hazards in water quality according to the prior art, flow cytometry (FCM) is used to quickly and in real time monitor the harmfulness of microorganisms in water quality, and to determine the characteristics of microbial community distribution within minutes for real-time monitoring. It is possible to estimate the quantitative amount of a specific microorganism by deriving the correlation between the measured value of flow cytometry and the measured value by the culture method.

However, in the case of a real-time monitoring device for microbial harmfulness in water quality according to the prior art, the measurement value by the culture method of the first measurement module 20 is the value of the flow cytometry (FCM) measurement value of the second measurement module 30. There is a hassle of deriving the correlation.

On the other hand, as another related technology, Korean Patent Registration No. 10-2150025 discloses an invention titled "Real-time detection sensor device for target microorganisms in tap water", which will be described with reference to FIG. 4 .

4 is a configuration diagram of a system for detecting target microorganisms in real time in tap water including a sensor device according to the prior art.

Referring to FIG. 4 , a system for detecting target microorganisms in real time in tap water including a sensor device according to the prior art includes a microorganism detection unit 50, a communication unit 60, a data storage unit 70, and a determination unit 80. do.

A microorganism loading unit 51 and a signal recognition unit are provided on the microorganism detection unit 50 that detects target microorganisms, and a hydrothermal synthesis unit and a signal disturbance blocking unit 52 are provided on the signal recognition unit, so that the microorganism seating unit The electrochemical reaction occurring in (51) is detected as an impedance signal value to measure or detect the target microorganism. At this time, the microorganisms include bacteria, Escherichia coli, etc., but are not limited thereto.

In addition, the microorganism detection unit 50 provided on the substrate is composed of a silicon dioxide wafer (SiO ₂ Wafer), and a microorganism seating unit 51 is formed at the center of the microorganism detection unit 50, and the microorganism seating unit 51 A signal recognition unit is formed along the circumference of.

According to the system for detecting target microorganisms in real time in tap water including a sensor device according to the prior art, bacteria or microorganisms that are detected below a standard value or are not detected in tap water can be detected and detected in real time.

However, in the case of a real-time detection system for target microorganisms in tap water including a sensor device according to the prior art, there is a limitation in that the target microorganisms must be measured or detected by detecting the electrochemical reaction occurring in the microorganism accommodation unit with an impedance signal value.

On the other hand, characterizing the organic and microbial matrices in water is a key issue for safe water supply, so the development of technologies that can provide convenient metrics of microbial water quality changes that enable continuous analysis of water quality, especially in water supply systems, is essential. It is an urgent situation.

Republic of Korea Patent Registration No. 10-1305993 (registration date: September 3, 2013), title of invention: "Method for measuring BOD using qualitative and quantitative analysis of organic matter" Republic of Korea Patent Registration No. 10-1735464 (registration date: May 8, 2017), title of invention: "Apparatus and method for measuring the existence of organic matter or life and death of organisms" Republic of Korea Patent Registration No. 10-1865587 (registration date: June 1, 2018), title of invention: "Apparatus and method for real-time monitoring of microbial hazards in water quality" Republic of Korea Patent Registration No. 10-2150025 (registration date: August 25, 2020), title of invention: "Sensor device for real-time detection of target microorganisms in tap water" Republic of Korea Patent Registration No. 10-972454 (registration date: July 20, 2010), title of invention: "Probe for measuring microorganisms in water quality using microbial fluorescence"

The technical problem to be achieved by the present invention for solving the above problems is to characterize the organic matter caused by microorganisms by measuring the organic matter caused by microorganisms in water using a continuous fluorescence spectroscopy to correspond to the continuously supplied sample. It is to provide a system and method for measuring organic matter caused by microorganisms using a continuous fluorescence analyzer capable of quantifying and monitoring water quality in real time.

Another technical problem to be achieved by the present invention is to measure the fluorescence intensity of a sample using an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm to measure tryptophan, an amino acid organic substance related to microbial activity. It is to provide a microorganism-derived organic matter measuring system and method using a continuous fluorescence spectrometer capable of calculating the population of Escherichia coli by doing so.

Another technical problem to be achieved by the present invention is to measure tryptophan, an organic amino acid, using an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm, which can calculate BOD5 used as a biological indicator. Thus, it is to provide a system and method for measuring organic matter originating from microorganisms using a continuous fluorescence spectrometer.

Another technical problem to be achieved by the present invention is to provide a continuous fluorescence analysis device capable of indirectly monitoring microbial contamination by continuously measuring tryptophan fluorescence intensity and generating and transmitting alarm information according to the microbial warning range. It is to provide a system and method for measuring organic matters caused by microorganisms.

As a means for achieving the above-described technical problem, the system for measuring organic matter caused by microorganisms using a continuous fluorescence analyzer according to the present invention includes a sample supply unit for continuously supplying samples so that they can be supplied at a flow rate that does not affect fluorescence analysis; a continuous type fluorescence analyzer for measuring samples continuously supplied from the sample supplier, calculating tryptophan concentration, E. coli population, or BOD5 concentration according to fluorescence intensity, and generating alarm information; And a manager terminal for receiving the alarm information transmitted through a wireless communication module of the continuous fluorescence analyzer, wherein the continuous fluorescence analyzer measures organic matter derived from microorganisms by fluorescence intensity representing tryptophan concentration, , Measure tryptophan fluorescence intensity according to a predetermined range of excitation wavelength and specific emission wavelength, calculate tryptophan concentration according to the tryptophan fluorescence intensity, and calculate E. coli population and BOD5 concentration from the calculated tryptophan concentration.

Here, the sample supply unit can continuously inject the sample at a flow rate that does not affect the fluorescence analysis by the continuous fluorescence analyzer by automatically controlling the sample supply pump.

Here, the continuous fluorescence analysis device includes: a continuous sample measurement unit for measuring fluorescence intensity of samples continuously supplied from the sample supply unit; a fluorescence intensity analysis unit that calculates tryptophan concentration, E. coli population, and BOD5 concentration according to the fluorescence intensity; an alarm information generation unit that determines whether or not to alarm according to the range of E. coli populations and generates alarm information; and a wireless communication module wirelessly transmitting the alarm information to a manager terminal.

Here, the continuous sample measurement unit includes a light source and an excitation monochromator, irradiates light using an ultraviolet light emitting diode as a light source, generates an excitation wavelength in the range of 270 nm to 290 nm through the excitation monochromator, , an excitation wavelength generator for incident light on the sample supplied through the sample supply unit; It includes an emission monochromator and a detector, and continuously detects a specific emission wavelength from light reflected from the sample, but when the emission monochromator, a band pass filter, passes a specific emission wavelength of 300 nm or 350 nm, a small spectrometer or an emission wavelength detector for detecting light of the specific emission wavelength using a detector such as a photodiode; And it may include a fluorescence intensity conversion unit for converting the emission wavelength detected through the detector of the emission wavelength detector into fluorescence intensity.

Here, the excitation wavelength generator and the emission wavelength detector may be installed at an angle of 90 degrees and connected through an optical fiber.

Here, the fluorescence intensity analysis unit, a tryptophan concentration calculator for calculating the tryptophan concentration from the fluorescence intensity measured according to the excitation wavelength in the range of 270 nm ~ 290 nm and the emission wavelength of 300 nm or 350 nm; E. coli population calculation unit for calculating the E. coli population by measuring the fluorescence intensity representing the tryptophan concentration according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm; a BOD5 determining unit for calculating a BOD5 concentration by measuring fluorescence intensity indicating a tryptophan concentration according to an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm; and a water quality risk determiner configured to determine a water quality risk by comparing values calculated based on the tryptophan concentration and the number of E. coli populations with a reference value, which is an E. coli risk risk range.

a]로 주어지며, a는 비례계수로서, 상기 a값은 UVLED 광원의 세기에 대응하는 형광강도에 따라 달라질 수 있다.Here, the tryptophan concentration calculated by the tryptophan concentration calculation unit is [(fluorescence intensity at an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm)

a], where a is a proportionality coefficient, and the a value may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source.

b]로 주어지며, b는 비례계수로서, 상기 b값은 UVLED 광원의 세기에 대응하는 형광강도에 따라 달라질 수 있다.Here, the continuous fluorescence analyzer measures and calculates the E. coli population by fluorescence intensity according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm, but the E. coli population is [(270 nm to 290 nm). fluorescence intensity at an excitation wavelength of nm and an emission wavelength of 300 nm or 350 nm)]

b], b is a proportionality coefficient, and the b value may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source.

c]로 주어지며, c는 비례계수로서, 상기 c값은 UVLED 광원의 세기에 대응하는 형광강도에 따라 달라질 수 있다.Here, the E. coli population calculated by the E. coli population calculation unit is [(tryptophan concentration)

c], c is a proportionality coefficient, and the c value may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source.

d + e]로 주어지며, 이때, d 및 e는 비례게수로서, 상기 d값 및 e값은 UVLED 광원의 세기에 대응하는 형광강도에 따라 달라질 수 있다.Here, the BOD5 concentration calculated in the BOD5 determination unit is [(tryptophan concentration)

d + e], where d and e are proportional coefficients, and the d and e values may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source.

Here, the E. coli risk range in the alarm information generating unit is "safe" for 0 objects/100ml, "slightly safe" for 0 to 1 object/100ml, and "slightly safe" for 1 to 10 objects/100ml according to the number of objects. Alarm information may be generated as "slightly dangerous", 10 to 100 objects/100ml as "dangerous", and 100 or more objects/100ml as "very dangerous".

Here, the alarm information generating unit may generate alarm information by calculating the number of Escherichia coli populations to indirectly indicate contamination by microorganisms when the tryptophan concentration is 10 μg/L or more.

On the other hand, as another means for achieving the above-described technical problem, the method for measuring organic matter caused by microorganisms using a continuous fluorescence analyzer according to the present invention includes a continuous sample measuring unit, a fluorescence intensity analysis unit, an alarm information generating unit and wireless communication A method for measuring organic matter originating from microorganisms using a continuous fluorescence spectrometer having a module, the method comprising the steps of: a) setting the flow rate of a continuously supplied sample and the intensity of a light source of a continuous sample measuring unit to be fixed; b) setting the excitation wavelength generator of the continuous sample measurement unit to generate an excitation wavelength within a predetermined range and the emission wavelength detector to detect a specific emission wavelength; c) continuously supplying a sample to the continuous sample measurement unit by a sample supply unit; d) measuring the continuously supplied sample by the continuous sample measuring unit and converting the detected emission wavelength into fluorescence intensity; e) calculating a tryptophan concentration, an E. coli population, and a BOD5 concentration according to the fluorescence intensity by the fluorescence intensity analyzer; f) determining a water quality risk by comparing the fluorescence intensity analysis unit with a preset tryptophan reference value and an E. coli population reference value; g) generating alarm information according to a water quality risk determination result by the alarm information generation unit; and h) transmitting alarm information to an administrator terminal through the wireless communication module, wherein the continuous fluorescence analysis device measures the organic matter derived from microorganisms by fluorescence intensity representing a tryptophan concentration, and excitation within a predetermined range is performed. It is characterized in that the tryptophan fluorescence intensity is measured according to the wavelength and the specific emission wavelength, the tryptophan concentration is calculated according to the tryptophan fluorescence intensity, and the E. coli population and BOD5 concentration can be calculated from the calculated tryptophan concentration.

Here, in step a), the continuous sample measurement unit of the continuous fluorescence spectrometer may adjust the intensity of the light source and control the flow rate in the sample supply pump in order to continuously measure the sample.

Here, in step a), the continuous fluorescence analyzer fixes the light source and flow rate, and if the fluorescence intensity is below the reference value when measured based on 10 μg/L of tryptophan, the light source is increased and the flow rate is adjusted to optimize the light source and flow rate. Microorganism-derived organic matter measurement system using a continuous fluorescence analyzer, characterized in that for controlling to find and measure.

Here, step a) may include: a-1) adjusting the intensity of a light source so that the excitation wavelength generator generates an excitation wavelength within a predetermined range and the emission wavelength detector detects a specific emission wavelength; a-2) adjusting the flow rate of the sample continuously supplied from the sample supply unit according to the control of the sample supply pump; a-3) measuring the fluorescence intensity of 10 μg/L tryptophan by the sample measuring unit; a-4) comparing whether the measured tryptophan fluorescence intensity is greater than a set value; and a-5) fixing the flow rate of the continuously supplied sample and the intensity of the light source when the tryptophan fluorescence intensity is greater than the set value, but when the tryptophan fluorescence intensity is less than the set value, the above a-1) to a-4) are repeated.

Here, the step g) may include g-1) generating alarm information according to the calculated number of E. coli populations; g-2) comparing whether the tryptophan concentration is greater than the reference value of 10 μg/L; and g-3) generating alarm information according to the tryptophan concentration when the tryptophan concentration is greater than the reference value, wherein the alarm information according to the E. coli population is safe, slightly safe, slightly dangerous, dangerous, and very It can be any one of the dangers.

According to the present invention, by measuring using the intrinsic fluorescence of tryptophan, an amino acid related to microbial activity, which is an organic matter originating from microorganisms in water, using a continuous fluorescence spectroscopy to correspond to continuously supplied samples, microorganisms Characterize and quantify causative organisms and monitor water quality in real time.

According to the present invention, in order to measure tryptophan, an amino acid related to microbial activity, the fluorescence intensity of the sample is measured using an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm to measure the E. coli population. can be calculated

According to the present invention, in order to measure tryptophan, an amino acid organic substance related to microbial activity, the fluorescence intensity of the sample is measured using an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm to calculate the population of Escherichia coli. can do.

Another technical problem to be achieved by the present invention is to measure tryptophan, an organic amino acid, using an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm, which can calculate BOD5 used as a biological indicator. Thus, it is to provide a system and method for measuring organic matter originating from microorganisms using a continuous fluorescence spectrometer.

According to the present invention, microbial contamination can be indirectly monitored by continuously measuring tryptophan fluorescence intensity, and alarm information according to a microbial warning range can be generated and transmitted.

1 is an operational flow chart showing a BOD measurement method using qualitative and quantitative analysis of organic matter according to the prior art.
2 is a configuration diagram of a life and death measuring device for measuring the existence of organic matter or the life and death of living things according to the prior art.
Figure 3 is a block diagram of a real-time monitoring device for microbial harmfulness in water quality according to the prior art.
4 is a configuration diagram of a system for detecting target microorganisms in real time in tap water including a sensor device according to the prior art.
5 is a block diagram of a system for measuring organic matter caused by microorganisms using a continuous fluorescence spectrometer according to an embodiment of the present invention.
FIG. 6 is a view for explaining in detail a continuous type sample measurement unit of the continuous type fluorescence spectrometer shown in FIG. 5 .
FIG. 7 is a detailed configuration diagram of a fluorescence intensity analysis unit of the continuous fluorescence analysis device shown in FIG. 5 .
8 is a view showing the tryptophan concentration corresponding to the fluorescence intensity detected by the continuous fluorescence analysis device and the E. coli population corresponding to the fluorescence intensity in the microorganism-derived organic matter measurement system using the continuous fluorescence analysis device according to an embodiment of the present invention. to be.
9 is a view showing the BOD5 concentration corresponding to the tryptophan concentration in the microorganism-derived organic matter measurement system using a continuous fluorescence analyzer according to an embodiment of the present invention.
10 is an operation flow chart showing a method for measuring organic matter originating from microorganisms using a continuous fluorescence spectrometer according to an embodiment of the present invention.
11 shows that the continuous fluorescence analyzer controls the supply of samples and adjusts the light source so that the continuous fluorescence analyzer can continuously and stably measure the fluorescence intensity in the method for measuring organic matter originating from microorganisms using the continuous fluorescence analyzer according to an embodiment of the present invention. It is an operation flow.
12 is an operation flow chart showing the generation of alarm information according to the detection of E. coli in the method for measuring organic matters caused by microorganisms using a continuous fluorescence analyzer according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily practice with reference to the accompanying drawings. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein. And in order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification.

Throughout the specification, when a certain component is said to "include", it means that it may further include other components without excluding other components unless otherwise stated. Also, terms such as “… unit” described in the specification refer to a unit that processes at least one function or operation, and may be implemented by hardware, software, or a combination of hardware and software.

[Microorganism-derived organic matter measurement system using a continuous fluorescence spectrometer]

Figure 5 is a configuration diagram of a microorganism-derived organic matter measuring system using a continuous fluorescence analyzer according to an embodiment of the present invention, and Figure 6 is a detailed description of the continuous sample measuring unit of the continuous fluorescence analyzer shown in FIG. FIG. 7 is a detailed configuration diagram of the fluorescence intensity analysis unit of the continuous fluorescence analysis device shown in FIG. 5 .

Referring to FIG. 5 , the system for measuring organic matters caused by microorganisms using a continuous fluorescence analyzer according to an embodiment of the present invention includes a sample supply unit 100, a continuous fluorescence analyzer 200, and a manager terminal 300, The continuous fluorescence analysis device 200 includes a continuous sample measurement unit 210, a fluorescence intensity analysis unit 220, an alarm information generation unit 230, and a wireless communication module 240.

The sample supply unit 100 continuously supplies samples to the continuous fluorescence analyzer 200 so that the samples can be supplied at a flow rate that does not affect fluorescence analysis. At this time, the flow rate of the sample may be controlled by controlling the operation of the sample supply pump 110 . That is, the sample supply unit 100 continuously injects the sample at a flow rate that does not affect the fluorescence analysis by the continuous fluorescence analyzer 200 by the automatic control of the sample supply pump 110 .

The continuous fluorescence analysis device 200 measures samples continuously supplied from the sample supply unit 100, calculates tryptophan concentration, E. coli population, or BOD5 concentration according to fluorescence intensity, and generates alarm information. At this time, the continuous fluorescence analyzer 200 measures the fluorescence intensity representing the concentration of tryptophan in organic substances derived from microorganisms, and measures the tryptophan fluorescence intensity according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm. The tryptophan concentration may be calculated according to the tryptophan fluorescence intensity, and at this time, the number of E. coli populations and the BOD5 concentration may be calculated from the calculated tryptophan concentration. In addition, the continuous fluorescence analyzer 200 may directly measure the E. coli population according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm.

Specifically, referring to FIG. 5 , the continuous sample measurement unit 210 of the continuous fluorescence analyzer 200 measures fluorescence intensity of a sample continuously supplied from the sample supply unit 100 .

More specifically, referring to FIG. 6, the continuous sample measuring unit 210 includes an excitation wavelength generator 211, an emission wavelength detector 212, and a fluorescence intensity conversion unit 213, and the excitation wavelength generator ( 211) includes a light source 211a and an excitation monochromator 211b, and the emission wavelength detector 212 may include an emission monochromator 212a and a detector 212b, but is not limited thereto.

The excitation wavelength generator 211 emits light using a light source 211a, for example, an ultraviolet light emitting diode (UVLED), and then, through the excitation monochromator 211b, for example, 270 nm to 270 nm. An excitation wavelength in the range of 290 nm is generated, and a light source is incident on the sample supplied through the sample supply unit 100.

The emission wavelength detector 212 continuously detects a specific emission wavelength from light reflected from the sample. Specifically, the emission wavelength detector 212 is composed of an emission monochromator 212a and a detector 212b, and the emission monochromator 212a, for example, a bandpass filter is 300 nm or 350 nm. When a specific emission wavelength of nm is passed, light of the specific emission wavelength is detected using a detector 212b such as a small spectrometer or a photodiode.

The fluorescence intensity conversion unit 213 converts the emission wavelength detected through the detector 212b of the emission wavelength detector 212 into fluorescence intensity.

Here, it is preferable that the excitation wavelength generator 211 and the emission wavelength detector 212 are installed at an angle of 90 degrees, and the excitation wavelength generator 211 and the emission wavelength detector 212 are connected by an optical fiber. it is desirable

Referring back to FIG. 5 , the fluorescence intensity analyzer 220 of the continuous fluorescence analyzer 200 calculates tryptophan concentration, E. coli population, and BOD5 concentration according to the fluorescence intensity. Specifically, as shown in FIG. 6, the fluorescence intensity analyzer 220 includes a tryptophan concentration calculator 221, an E. coli population calculator 222, a BOD5 determiner 223, and a water quality risk determiner 224. ) may be included.

a]로 주어지며, 이때, a는 비례계수로서, 예를 들면, 0.2787로 산출될 수 있지만, 상기 a값은 UVLED 광원(211a)의 세기에 대응하는 형광강도에 따라 달라질 수 있다.The tryptophan concentration calculation unit 221 of the fluorescence intensity analyzer 220 calculates the tryptophan concentration from the fluorescence intensity measured according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm. At this time, the tryptophan concentration [unit: μg / L] is [(fluorescence intensity at an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm)

a], where a is a proportionality coefficient, for example, can be calculated as 0.2787, but the a value may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a.

b]로 주어지며, 이때, b는 비례계수로서, 예를 들면, b는 72.074로 주어질 수 있지만, 상기 b값은 UVLED 광원(211a)의 세기에 대응하는 형광강도에 따라 달라질 수 있다.In addition, the continuous fluorescence analyzer 200 may measure and calculate the E. coli population by fluorescence intensity according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm. At this time, the E. coli population [unit: population / ml] is [(fluorescence intensity at an excitation wavelength of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm)]

b], where b is a proportionality coefficient, for example, b may be given as 72.074, but the b value may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a.

c]로 주어지며, 이때, c = 258.61로 주어지되, 상기 c값은 UVLED 광원(211a)의 세기에 대응하는 형광강도에 따라 달라질 수 있다.In addition, the E. coli population calculation unit 222 of the fluorescence intensity analysis unit 220 measures the fluorescence intensity representing the tryptophan concentration according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm, thereby counting the E. coli population. can be calculated. At this time, the E. coli population [unit: population / ml] is [(tryptophan concentration)

c], where c = 258.61, but the c value may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a.

d + e]로 주어지며, 이때, d = 0.0839, e = -5.7534로 주어지되, 상기 d값 및 e값은 UVLED 광원(211a)의 세기에 대응하는 형광강도에 따라 달라질 수 있다.In addition, the BOD5 determination unit 223 of the fluorescence intensity analysis unit 220 measures the fluorescence intensity representing the tryptophan concentration according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm to determine the BOD5 concentration. can be calculated At this time, the BOD5 concentration [mg / L] is [(tryptophan concentration)

d + e], where d = 0.0839 and e = -5.7534, but the d and e values may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a.

The water quality risk determination unit 224 of the fluorescence intensity analysis unit 220 determines the water quality risk by comparing the values calculated from the tryptophan concentration and the E. coli population with a reference value that is within the E. coli risk range. For example, the risk range of Escherichia coli risk (Risk) is 0 population / 100 ml, 0 to 1 population / 100 ml range, 1 to 10 population / 100 ml range, 10 to 100 population / 100 ml range, and It can be divided into a population of 100 or more / 100 ml.

In addition, the alarm information generation unit 230 of the continuous fluorescence analyzer 200 determines whether or not to alarm according to the E. coli population range and generates alarm information. Here, the alarm information generating unit 230 compares the continuously measured fluorescence intensity with the value calculated as the tryptophan concentration and the E. coli population in the fluorescence intensity analysis unit 220 with the reference value, which is the risk range of E. coli risk. Alarm information is generated according to the method, and then transmitted wirelessly to the manager terminal 300 through the wireless communication module 240 in real time. For example, in the alarm information generating unit 230, the E. coli risk range is "safe" for 0 object/100 ml, "slightly safe" for 0 to 1 object/100 ml, and 1 to 10 object/100 ml according to the number of objects. Alarm information can be generated as “slightly dangerous” in the range of 100 ml, “dangerous” in the range of 10 to 100 objects/100 ml, and “very dangerous” in the range of 100 or more objects/100 ml.

In addition, when the concentration of tryptophan is 10 μg/L or more, the alarm information generation unit 230 may calculate the number of E. coli populations and generate alarm information so as to indirectly indicate contamination by microorganisms.

The wireless communication module 240 of the continuous fluorescence analyzer 200 wirelessly transmits the alarm information to the manager terminal 300 in real time.

The manager terminal 300 receives the alarm information transmitted through the wireless communication module 240 of the continuous fluorescence analyzer 200 .

On the other hand, Figure 8 shows the tryptophan concentration corresponding to the fluorescence intensity detected by the continuous fluorescence analysis device and the E. coli population corresponding to the fluorescence intensity in the microorganism-derived organic matter measurement system using the continuous fluorescence analysis device according to an embodiment of the present invention. FIG. 9 is a diagram showing the BOD5 concentration corresponding to the tryptophan concentration in the microorganism-derived organic matter measuring system using a continuous fluorescence analyzer according to an embodiment of the present invention.

In the case of the system for measuring organic matter derived from microorganisms using a continuous fluorescence spectrometer according to an embodiment of the present invention, the concentration of tryptophan, an organic matter derived from microorganisms, is used as a convenient measurement index of microorganisms present in water, and a predetermined range of excitation wavelength and specific emission are used. By using a continuous-type fluorescence spectrometer that measures samples in real time by wavelength, the concentration of organic matter caused by microorganisms and the number of E.coli (E.coli) can be quantified.

a]로 주어지며, 이때, a는 비례계수로서, 예를 들면, 0.2787로 산출될 수 있지만, 상기 a값은 UVLED 광원(211a)의 세기에 대응하는 형광강도에 따라 달라질 수 있다.In particular, by using tryptophan, an organic substance derived from microorganisms, as a measurement index for a water supply system in which microorganisms may occur in relation to water, it is used to monitor the concentration of organic substances caused by microorganisms, the number of Escherichia coli and BOD5 as indicators, as shown in FIG. Similarly, the continuous fluorescence analyzer 200 calculates the tryptophan concentration from the fluorescence intensity measured according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm. At this time, the tryptophan concentration [unit: μg / L] is [(fluorescence intensity at an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm)

a], where a is a proportionality coefficient, for example, can be calculated as 0.2787, but the a value may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a.

b]로 주어지며, 이때, b는 비례계수로서, 예를 들면, b는 72.074로 주어질 수 있지만, 상기 b값은 UVLED 광원(211a)의 세기에 대응하는 형광강도에 따라 달라질 수 있다.In addition, the continuous fluorescence analyzer 200 may measure and calculate the E. coli population by fluorescence intensity according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm. At this time, the E. coli population [unit: population / ml] is [(fluorescence intensity at an excitation wavelength of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm)]

b], where b is a proportionality coefficient, for example, b may be given as 72.074, but the b value may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a.

Accordingly, the quality of water can be monitored in real time by characterizing and quantifying the organic matter originating from microorganisms by measuring organic matter originating from microorganisms using a continuous fluorescence spectrometer to correspond to samples continuously supplied. In addition, by using tryptophan as an organic matter derived from microorganisms in water as a measurement index, it can be safely measured as non-pathogenic without being specific to any pathogen, and can be used as a tool for water quality monitoring, and the number of E. coli populations can be calculated by calculating the tryptophan concentration in water. can do.

d + e]로 주어지며, 이때, d = 0.0839, e = -5.7534로 주어지되, 상기 d값 및 e값은 UVLED 광원(211a)의 세기에 대응하는 형광강도에 따라 달라질 수 있다.In addition, as shown in FIG. 9, the continuous fluorescence analyzer 200 measures the fluorescence intensity representing the concentration of tryptophan according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm to measure the BOD5 concentration can be calculated. At this time, the BOD5 concentration [mg / L] is [(tryptophan concentration)

d + e], where d = 0.0839 and e = -5.7534, but the d and e values may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a.

That is, the concentration of tryptophan in water can be calculated and used as an indicator of BOD5, and can be used as a tool for monitoring water quality.

After all, according to an embodiment of the present invention, the intrinsic fluorescence of tryptophan, an amino acid related to microbial activity, which is an organic matter caused by microorganisms in water, is used by using a continuous fluorescence spectroscopy to correspond to continuously supplied samples. By measuring it, it is possible to characterize and quantify organic matter caused by microorganisms and monitor water quality in real time. In addition, in order to measure tryptophan, an amino acid related to the activity of microorganisms, which is an organic substance, the fluorescence intensity of the sample can be measured using an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm to calculate the E. coli population. there is.

[Method for measuring organic substances caused by microorganisms using a continuous fluorescence spectrometer]

10 is an operation flow chart showing a method for measuring organic matter originating from microorganisms using a continuous fluorescence spectrometer according to an embodiment of the present invention.

Referring to FIG. 10 , the method for measuring organic matters caused by microorganisms using a continuous fluorescence analyzer according to an embodiment of the present invention includes a continuous sample measurement unit 210, a fluorescence intensity analysis unit 220, and an alarm information generation unit 230. ) and a wireless communication module 240 as a method for measuring organisms originating from microorganisms using a continuous fluorescence analyzer 200, first, the flow rate of continuously supplied samples and the intensity of the light source of the continuous sample measuring unit 210 are fixed. It is set to be (S110).

Next, the excitation wavelength generator 211 of the continuous sample measuring unit 210 generates an excitation wavelength within a predetermined range, and the emission wavelength detector 212 is set to detect a specific emission wavelength (S120).

Next, the sample supply unit 100 continuously supplies samples to the continuous sample measurement unit 210 (S130). That is, the sample supply unit 100 continuously injects the sample at a flow rate that does not affect the fluorescence analysis by the continuous fluorescence analyzer 200 by the automatic control of the sample supply pump 110 .

Next, the continuous sample measuring unit 210 measures the continuously supplied sample and converts the detected emission wavelength into fluorescence intensity (S140). At this time, the continuous sample measuring unit 210 measures the fluorescence intensity representing the concentration of tryptophan in the microbial-derived organic material, and measures the tryptophan fluorescence intensity according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm. In addition, the continuous fluorescence analyzer 200 may directly measure the E. coli population according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm.

Next, the fluorescence intensity analyzer 220 calculates tryptophan concentration, E. coli population, and BOD5 concentration according to the fluorescence intensity (S150). That is, the tryptophan concentration can be calculated according to the tryptophan fluorescence intensity, and at this time, the number of E. coli populations and the BOD5 concentration can be calculated from the calculated tryptophan concentration.

a]로 주어지며, 이때, a는 비례계수로서, 예를 들면, 0.2787로 산출될 수 있지만, 상기 a값은 UVLED 광원(211a)의 세기에 대응하는 형광강도에 따라 달라질 수 있다.Specifically, the tryptophan concentration calculation unit 221 of the fluorescence intensity analysis unit 220 calculates the tryptophan concentration from the fluorescence intensity measured according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm. . At this time, the tryptophan concentration [unit: μg / L] is [(fluorescence intensity at an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm)

a], where a is a proportionality coefficient, for example, can be calculated as 0.2787, but the a value may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a.

b]로 주어지며, 이때, b는 비례계수로서, 예를 들면, b는 72.074로 주어질 수 있지만, 상기 b값은 UVLED 광원(211a)의 세기에 대응하는 형광강도에 따라 달라질 수 있다.In addition, the continuous fluorescence analyzer 200 may measure and calculate the E. coli population by fluorescence intensity according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm. At this time, the E. coli population [unit: population / ml] is [(fluorescence intensity at an excitation wavelength of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm)]

b], where b is a proportionality coefficient, for example, b may be given as 72.074, but the b value may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a.

c]로 주어지며, 이때, c = 258.61로 주어지되, 상기 c값은 UVLED 광원(211a)의 세기에 대응하는 형광강도에 따라 달라질 수 있다.In addition, the E. coli population calculation unit 222 of the fluorescence intensity analysis unit 220 measures the fluorescence intensity representing the tryptophan concentration according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm, thereby counting the E. coli population. can be calculated. At this time, the E. coli population [unit: population / ml] is [(tryptophan concentration)

c], where c = 258.61, but the c value may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a.

d + e]로 주어지며, 이때, d = 0.0839, e = -5.7534로 주어지되, 상기 d값 및 e값은 UVLED 광원(211a)의 세기에 대응하는 형광강도에 따라 달라질 수 있다.In addition, the BOD5 determination unit 223 of the fluorescence intensity analysis unit 220 measures the fluorescence intensity representing the tryptophan concentration according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm to determine the BOD5 concentration. can be calculated At this time, the BOD5 concentration [mg / L] is [(tryptophan concentration)

d + e], where d = 0.0839 and e = -5.7534, but the d and e values may vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a.

Next, the fluorescence intensity analyzer 220 compares the preset tryptophan reference value and E. coli population reference value, respectively, to determine the water quality risk (S160). That is, the water quality risk is determined by comparing the values calculated from the tryptophan concentration and the E. coli population with a reference value that is within the E. coli risk range. For example, the risk range of Escherichia coli risk (Risk) is 0 population / 100 ml, 0 to 1 population / 100 ml range, 1 to 10 population / 100 ml range, 10 to 100 population / 100 ml range, and It can be divided into a population of 100 or more / 100 ml.

Next, the alarm information generation unit 230 generates alarm information according to the water quality risk determination result (S170). Here, the alarm information generating unit 230 compares the continuously measured fluorescence intensity with the value calculated as the tryptophan concentration and the E. coli population in the fluorescence intensity analysis unit 220 with the reference value, which is the risk range of E. coli risk. Alarm information is generated according to the method, and then transmitted wirelessly to the manager terminal 300 through the wireless communication module 240 in real time. For example, in the alarm information generating unit 230, the E. coli risk range is "safe" for 0 object/100 ml, "slightly safe" for 0 to 1 object/100 ml, and 1 to 10 object/100 ml according to the number of objects. Alarm information can be generated as “slightly dangerous” in the range of 100 ml, “dangerous” in the range of 10 to 100 objects/100 ml, and “very dangerous” in the range of 100 or more objects/100 ml. In addition, when the concentration of tryptophan is 10 μg/L or more, the alarm information generation unit 230 may calculate the number of E. coli populations and generate alarm information so as to indirectly indicate contamination by microorganisms.

Next, alarm information is transmitted to the manager terminal 300 through the wireless communication module 240 (S180).

On the other hand, Figure 11 is a method for measuring organic matter originating from microorganisms using a continuous fluorescence analyzer according to an embodiment of the present invention, wherein the continuous fluorescence analyzer controls sample supply and adjusts the light source so that the fluorescence intensity can be continuously and stably measured. It is a flow chart showing the operation.

Referring to FIG. 11, in the method for measuring organic matter caused by microorganisms using a continuous fluorescence spectrometer according to an embodiment of the present invention, the flow rate of the continuously supplied sample and the intensity of the light source of the continuous sample measuring unit 210 are set to be fixed. Specifically, the excitation wavelength generator 211 generates an excitation wavelength within a predetermined range, and the emission wavelength detector 212 adjusts the intensity of the light source to detect a specific emission wavelength (S111).

Next, the flow rate of the sample continuously supplied from the sample supply unit 100 is adjusted according to the control of the sample supply pump 110 (S112).

Next, the sample measuring unit 210 measures the fluorescence intensity of 10 μg/L tryptophan (S113).

Next, it is compared whether the measured tryptophan fluorescence intensity is greater than the set value (S114). At this time, when the tryptophan fluorescence intensity is less than the set value, steps S111 to S114 are repeated.

Next, when the tryptophan fluorescence intensity is greater than the set value, the flow rate of the continuously supplied sample and the intensity of the light source are fixed.

Accordingly, the sample supply can be controlled in advance and the light source can be adjusted so that the continuous fluorescence analyzer 200 can continuously and stably measure the fluorescence intensity. That is, in order to continuously measure the sample, it is necessary to adjust the light source and control the flow rate of the sample supply pump 110. For example, when the light source and flow rate are fixed and measured based on 10 μg/L of tryptophan, fluorescence If the intensity is less than the standard value, the light source may be increased and the flow rate may be adjusted to find and measure the optimal light source and flow rate.

Meanwhile, FIG. 12 is an operation flow chart illustrating generation of alarm information according to detection of E. coli in the method for measuring organic matters caused by microorganisms using a continuous fluorescence analyzer according to an embodiment of the present invention.

Referring to FIG. 12, in the method for measuring organic substances derived from microorganisms using a continuous fluorescence analyzer according to an embodiment of the present invention, as described above, after measuring the tryptophan fluorescence intensity of the sample (S140), the tryptophan concentration and the number of E. coli populations After calculating (S150), it is possible to generate alarm information according to the calculated E. coli population or generate alarm information according to the tryptophan concentration.

Specifically, alarm information according to the calculated E. coli population is generated (S161). For example, the alarm information according to the population of Escherichia coli may be any one of safe, slightly safe, slightly dangerous, dangerous, and very dangerous.

Next, whether the tryptophan concentration is greater than a reference value, for example, 10 μg/L is compared (S162).

Next, when the tryptophan concentration is greater than the reference value, alarm information according to the tryptophan concentration is generated (S163). Subsequently, alarm information according to the calculated E. coli population or alarm information according to the tryptophan concentration is transmitted to the manager terminal 300 (S170).

In other words, if the tryptophan concentration is 10 μg/L or more, contamination by microorganisms is indirectly indicated, and the number of E. coli populations is calculated and alarm information is transmitted.

After all, according to an embodiment of the present invention, contamination of microorganisms can be indirectly monitored by continuously measuring tryptophan fluorescence intensity, and alarm information according to the warning range of microorganisms can be generated and transmitted.

The above description of the present invention is for illustrative purposes, and those skilled in the art can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims rather than the detailed description above, and all changes or modifications derived from the meaning and scope of the claims and equivalent concepts should be construed as being included in the scope of the present invention. do.

100: sample supply unit 200: continuous fluorescence analysis device
300: manager terminal 110: sample supply pump
210: continuous sample measurement unit 220: fluorescence intensity analysis unit
230: alarm information generating unit 240: wireless communication module
211: excitation wavelength generator 212: emission wavelength detector
213: fluorescence intensity conversion unit
221: tryptophan concentration calculation unit 222: E. coli population calculation unit
223: BOD5 determination unit 224: water quality risk determination unit
211a UVLED light source 211b excitation monochromator
212a: emission monochromator 212b: detector

Claims (23)

A sample supply unit 100 for continuously supplying a sample so that it can be supplied at a flow rate that does not affect fluorescence analysis;
A continuous type fluorescence analysis device 200 for measuring samples continuously supplied from the sample supply unit 100, calculating tryptophan concentration, E. coli population, or BOD5 concentration according to fluorescence intensity, and generating alarm information; and
Including a manager terminal 300 for receiving the alarm information transmitted through the wireless communication module 240 of the continuous fluorescence analyzer 200,
The continuous fluorescence analyzer 200 measures the fluorescence intensity representing the concentration of tryptophan in organic matter derived from microorganisms, measures the tryptophan fluorescence intensity according to a predetermined range of excitation wavelength and a specific emission wavelength, and calculates the tryptophan fluorescence intensity. Microbial-derived organic matter measurement system using a continuous fluorescence analyzer, characterized in that the tryptophan concentration is calculated according to the calculated tryptophan concentration, and the E. coli population and the BOD5 concentration are calculated. According to claim 1,
Continuous-type fluorescence analysis, characterized in that the sample supply unit 100 continuously injects the sample at a flow rate that does not affect the fluorescence analysis by the continuous-type fluorescence analyzer 200 by automatic control of the sample supply pump 110 A system for measuring organic matter caused by microorganisms using a device. The method of claim 1, wherein the continuous fluorescence analysis device 200,
a continuous sample measurement unit 210 for measuring fluorescence intensity of samples continuously supplied from the sample supply unit 100;
Fluorescence intensity analyzer 220 for calculating tryptophan concentration, E. coli population and BOD5 concentration according to the fluorescence intensity;
an alarm information generation unit 230 for generating alarm information by determining whether or not to alarm according to the range of E. coli population; and
Microbial-derived organic matter measurement system using a continuous fluorescence analysis device including a wireless communication module 240 for wirelessly transmitting the alarm information to the manager terminal 300. The method of claim 3, wherein the continuous sample measuring unit 210,
It includes a light source 211a and an excitation monochromator 211b, and irradiates light using an ultraviolet light emitting diode (UVLED) as the light source 211a, and through the excitation monochromator 211b, a range of 270 nm to 290 nm an excitation wavelength generator 211 generating an excitation wavelength of and incident a light source on the sample supplied through the sample supply unit 100;
It includes an emission monochromator 212a and a detector 212b, and continuously detects a specific emission wavelength from the light reflected from the sample, and the bandpass filter of the emission monochromator 212a is 300 nm or an emission wavelength detector 212 for detecting light of the specific emission wavelength using a detector 212b such as a small spectrometer or a photodiode when a specific emission wavelength of 350 nm is passed; and
Microbial-derived organic matter measurement system using a continuous fluorescence analysis device including a fluorescence intensity conversion unit 213 for converting the emission wavelength detected through the detector 212b of the emission wavelength detector 212 into fluorescence intensity. According to claim 4,
The excitation wavelength generator 211 and the emission wavelength detector 212 are installed at an angle of 90 degrees and connected by an optical fiber. The method of claim 3, wherein the fluorescence intensity analyzer 220,
Tryptophan concentration calculator 221 for calculating the tryptophan concentration from the fluorescence intensity measured according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm;
E. coli population calculation unit 222 for calculating the E. coli population by measuring fluorescence intensity representing the concentration of tryptophan according to an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm;
a BOD5 determination unit 223 for calculating a BOD5 concentration by measuring fluorescence intensity indicating a tryptophan concentration according to an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm; and
Microbial-derived organic matter measurement system using a continuous fluorescence spectrometer including a water quality risk determination unit 224 for determining a water quality risk by comparing the value calculated from the tryptophan concentration and the E. coli population with a reference value, which is an E. coli risk range. System. According to claim 6,
The tryptophan concentration [unit: μg/L] calculated by the tryptophan concentration calculation unit 221 is [(fluorescence intensity at an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm)

a], where a is a proportionality coefficient, and the a value varies depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a. According to claim 6,
The continuous fluorescence analyzer 200 measures and calculates the E. coli population by fluorescence intensity according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm, and the E. coli population [Unit: population / ml ] is [(fluorescence intensity at an excitation wavelength of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm)]

b], b is a proportionality coefficient, and the b value varies according to the fluorescence intensity corresponding to the intensity of the UVLED light source 211a. According to claim 6,
The E. coli population calculated by the E. coli population calculation unit 222 [unit: population/ml] is [(tryptophan concentration)

c], c is a proportionality coefficient, and the c value varies according to the fluorescence intensity corresponding to the intensity of the UVLED light source 211a. According to claim 6,
The BOD5 concentration [mg/L] calculated by the BOD5 determination unit 223 is [(tryptophan concentration)

d + e], d and e are proportional coefficients, and the d and e values vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a. Causal organic matter measurement system. According to claim 6,
In the alarm information generation unit 230, the E. coli risk range is "safe" for 0 objects/100ml, "slightly safe" for 0 to 1 object/100ml, and "slightly safe" for 1 to 10 objects/100ml. "Slightly dangerous", 10 to 100 objects / 100㎖ is "dangerous", 100 or more objects / 100㎖ is "very dangerous" Microorganisms using a continuous fluorescence analyzer characterized in that it generates alarm information Causal organic matter measurement system. According to claim 6,
The alarm information generator 230 calculates the number of Escherichia coli populations and generates alarm information so that contamination by microorganisms can be indirectly indicated when the tryptophan concentration is 10 μg/L or more. A system for measuring organic matter originating from microorganisms used. A method for measuring organic substances caused by microorganisms using a continuous fluorescence analyzer 200 equipped with a continuous sample measuring unit 210, a fluorescence intensity analysis unit 220, an alarm information generating unit 230, and a wireless communication module 240 in
a) setting the flow rate of the continuously supplied sample and the intensity of the light source of the continuous sample measuring unit 210 to be fixed;
b) setting the excitation wavelength generator 211 of the continuous sample measuring unit 210 to generate an excitation wavelength within a predetermined range and the emission wavelength detector 212 to detect a specific emission wavelength;
c) continuously supplying, by the sample supply unit 100, samples to the continuous sample measurement unit 210;
d) the continuous sample measuring unit 210 measures the continuously supplied sample and converts the detected emission wavelength into fluorescence intensity;
e) calculating, by the fluorescence intensity analyzer 220, tryptophan concentration, E. coli population, and BOD5 concentration according to the fluorescence intensity;
f) determining, by the fluorescence intensity analyzer 220, a water quality risk by comparing a preset tryptophan reference value and an E. coli population reference value;
g) generating, by the alarm information generation unit 230, alarm information according to a water quality risk determination result; and
h) transmitting alarm information to the manager terminal 300 through the wireless communication module 240,
The continuous fluorescence analyzer 200 measures the fluorescence intensity representing the concentration of tryptophan in organic matter derived from microorganisms, measures the tryptophan fluorescence intensity according to a predetermined range of excitation wavelength and a specific emission wavelength, and calculates the tryptophan fluorescence intensity. A method for measuring organic substances caused by microorganisms using a continuous fluorescence analyzer, characterized in that the tryptophan concentration is calculated according to the calculated tryptophan concentration, and the E. coli population and BOD5 concentration are calculated. According to claim 13,
In step a), the continuous sample measurement unit 210 of the continuous fluorescence spectrometer 200 adjusts the light source intensity and controls the flow rate in the sample supply pump 110 to continuously measure the sample. A method for measuring organic matter originating from microorganisms using a continuous fluorescence spectrometer. According to claim 13,
In the step a), the continuous fluorescence analyzer 200 fixes the light source and the flow rate, and if the fluorescence intensity is below the reference value when measured based on 10 μg/L of tryptophan, the light source is increased and the flow rate is adjusted to obtain an optimal light source and Microbial-derived organic matter measurement system using a continuous fluorescence analyzer, characterized in that for controlling to find and measure the flow rate. The method of claim 13, wherein step a),
a-1) adjusting the intensity of a light source so that the excitation wavelength generator 211 generates an excitation wavelength within a predetermined range and the emission wavelength detector 212 detects a specific emission wavelength;
a-2) adjusting the flow rate of the sample continuously supplied from the sample supply unit 100 according to the control of the sample supply pump 110;
a-3) measuring the fluorescence intensity of 10 μg/L tryptophan by the sample measuring unit 210;
a-4) comparing whether the measured tryptophan fluorescence intensity is greater than a set value; and
a-5) when the tryptophan fluorescence intensity is greater than the set value, fixing the flow rate of the continuously supplied sample and the intensity of the light source,
When the tryptophan fluorescence intensity is less than the set value, the step a-1) to a-4) is repeatedly performed. According to claim 13,
The tryptophan concentration [unit: μg/L] calculated in step e) is [(fluorescence intensity at an excitation wavelength in the range of 270 nm to 290 nm and an emission wavelength of 300 nm or 350 nm)

a], where a is a proportionality coefficient, and the a value varies depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a. According to claim 13,
In step e), the E. coli population is measured by fluorescence intensity according to the excitation wavelength in the range of 270 nm to 290 nm and the emission wavelength of 300 nm or 350 nm, and the E. coli population [unit: population / ml] is [(270 Fluorescence intensity at an excitation wavelength of ∼290 nm and an emission wavelength of 300 nm or 350 nm)]

b], b is a proportionality coefficient, and the b value varies according to the fluorescence intensity corresponding to the intensity of the UVLED light source 211a. According to claim 13,
The number of E. coli populations calculated in step e) [unit: population/ml] is [(tryptophan concentration)

c], c is a proportionality coefficient, and the c value varies depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a. According to claim 13,
The BOD5 concentration [mg/L] calculated in step e) is [(tryptophan concentration)

d + e], d and e are proportional coefficients, and the d and e values vary depending on the fluorescence intensity corresponding to the intensity of the UVLED light source 211a. Methods for measuring causal organic matter. According to claim 13,
In step g), alarm information according to the calculated E. coli population or alarm information according to the tryptophan concentration is generated. The method of claim 13, wherein step g),
g-1) generating alarm information according to the calculated E. coli population;
g-2) comparing whether the tryptophan concentration is greater than the reference value of 10 μg/L; and
g-3) generating alarm information according to the tryptophan concentration when the tryptophan concentration is greater than a reference value;
The alarm information according to the number of Escherichia coli is safe, slightly safe, slightly dangerous, dangerous, and very dangerous. The method of claim 22,
According to the E. coli population range in step g-1), 0 population/100 ml is "safe", 0 to 1 population/100 ml is "slightly safe", and 1 to 10 population/100 ml is "slightly dangerous". , 10 ~ 100 population / 100㎖ range is "dangerous", 100 or more population / 100㎖ generates alarm information as "very dangerous" Method for measuring organic matter caused by microorganisms using a continuous fluorescence spectrometer.

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