KR100972454B1 - Microorganism mesuring probe using microorganism fluorescence - Google Patents

Abstract

The present invention relates to a probe for measuring microorganisms, and more particularly, to a probe for measuring microorganisms capable of detecting even microorganisms and ultra-fine particles contained in a sample water and accurately measuring turbidity.

The microbial measurement probe of the present invention is a light emitting unit having a cylindrical housing, a first lamp which is embedded in the housing and emits ultraviolet light for detecting microorganisms contained in the sample water, and is emitted from the light emitting unit to the outside of the housing. A photodetector for detecting light or fluorescence scattered by the number of samples is provided.

According to the probe for measuring microorganisms of the present invention, by embedding an ultraviolet diode, an infrared diode, and a laser diode in the probe, particles and microorganisms of various sizes in the water can be precisely detected with a single probe, which makes measurement simple and accurate, based on turbidity. Provide a system.

Turbidity, Ultrafine Particles, Particles, Microorganisms, Lasers, Near Infrared, Probes

Description

Microorganism mesuring probe using microorganism fluorescence}

The present invention relates to a probe for measuring microorganisms, and more particularly, to a probe for measuring microorganisms capable of detecting even microorganisms and ultra-fine particles contained in a sample water and accurately measuring turbidity.

Due to serious environmental pollution, research on the measurement and removal of water quality and air pollution is indispensable and active.

Above all, the measurement and improvement of the pollution level of water, which is the source of humans and all living things, is the most important.In order to judge water pollution, biological characteristics of the water environment, chemical properties such as trace elements in suspended solids, and water color, smell, turbidity The physical characteristics of the should be considered.

Among them, especially turbidity is a quantitative indicator of the cloudiness of water and is expressed as scattering and absorption by light of particles suspended in water. This turbidity is caused by various suspended substances and the size of suspended particles in the water varies from colloidal dispersion to coarse dispersion. This includes nanoscale ultrafine particles.

Turbidity is caused by extremely fine dispersoids, such as colloidal dispersion, in water in relatively stagnant conditions, such as lakes, and by coarse dispersoids, mostly in flowing water, such as river water.

Particulates that form sediment or suspended solids are the main pollutants that affect the environment, such as water sources and lakes, and these particles flow into the water supply pipes and cause problems such as erosion or deposition inside the pipes. In addition, metal fine particles are generated by the corrosion of the surface of the water pipes made of metal pipes, which may cause corrosion of the pipes and cause erosion at the same time. Particles in the pipe form scales on the inner surface of the pipe, disrupting the flow of fluid inside the pipe, and particles smaller than 64 μm that cause turbidity can directly damage biological activity or cause harmful chemicals. It can also be a means of delivery.

And when the water quality is contaminated by colloids or dispersoids, the transparency of the water changes, so that the degree of contamination can be easily known.

These ultrafine particles include a variety of metals, organic substances, viruses, algae and mold, and carcinogenic substances such as polycyclic aromatic hydrocarbons (PAH). In particular, ultra-fine particles consist of metal elements such as heavy metals, so it is urgent to develop measurement methods and monitoring technology.

In addition, organic matter is introduced into the water quality and the number of microorganisms increases, causing biological contamination. In this case, especially in drinking water management, monitoring of microorganisms is also a very important measurement variable.

Therefore, when measuring turbidity to evaluate water quality, ultra-fine particles or microorganisms among suspended solids in water should be accurately detected.

However, conventional turbidity measuring devices are configured to detect turbidity using a tungsten filament lamp or an infrared LED as a light source. Such a longitudinal light source emits only light having a wavelength in the ultraviolet band and thus cannot selectively detect particles of various sizes in the water, and it is not easy to detect particles smaller than 6 μm with a low energy beam such as an infrared LED. There is a problem. In addition, the tungsten lamp has a problem that the life is very short, such as 2000 hours, so it must be replaced frequently.

In addition, the conventional turbidity measuring device has a problem that microorganisms present in the water can not be detected at all.

The present invention has been made to improve the above problems, and an object of the present invention is to provide a probe for measuring microorganisms that can detect ultrafine particles or microorganisms in water so that water quality can be accurately analyzed.

Another object of the present invention is to provide an integrated probe capable of selectively detecting suspended substances or microorganisms in water with one probe.

Probe for measuring the microorganism of the present invention for achieving the above object is a cylindrical housing; A light emitting unit embedded in the housing and having a first lamp for emitting ultraviolet light for detecting microorganisms contained in the sample water; And a photo detector emitted from the light emitting unit to the outside of the housing to detect light or fluorescence scattered by the sample water.

The first lamp is an ultraviolet diode that emits light having a wavelength of 330 to 350nm.

The light emitting unit further includes a second lamp that emits laser light to detect particulates contained in the sample water, and a third lamp that emits infrared light to detect general turbidity, wherein the light emitting unit is one of the lamps. It is characterized in that either one is selectively driven.

The second lamp is a laser diode that emits light of a wavelength of 830nm, the third lamp is characterized in that the near-infrared diode that emits light of a wavelength of 820 to 900nm.

A light emitting optical fiber for transferring the light emitted from the light emitting part to the sample water located outside the housing; And a light receiving optical fiber which transmits light incident from the sample water into the housing to the photodetector.

On one side of the bottom surface of the housing, the light emitted from the end of the light emitting optical fiber is projected to the sample water, and the light is transmitted at right angles to the optical axis emitted from the light emitting window is incident to the end of the light receiving optical fiber And a light receiving window formed on the other side of the bottom of the housing.

And a wiper installed at an outer side of a bottom of the housing so as to be driven by the driving motor and to clean the light emitting window and the light receiving window.

In addition, the turbidity measuring device of the present invention for achieving the above object is a cylindrical housing, and for detecting the first lamp and ultra-fine particles for emitting ultraviolet light for detecting microorganisms contained in the sample water contained in the housing A light emitting portion having a second lamp for emitting laser light and a third lamp for emitting infrared light for detecting general turbidity; and light or fluorescence emitted from the light emitting portion to the outside of the housing and scattered by the sample water. A turbidity measurement probe having a photodetector for detecting; An operation unit for selectively driving any one of the lamps of the light emitting unit; A controller for controlling the lamps and calculating and processing a signal input from the photodetector to output a constant electrical signal; And a display unit for displaying the electrical signal output from the controller.

As described above, according to the probe for measuring microorganisms of the present invention, the amount of microorganisms contained in the sample water is accurately detected by irradiating ultraviolet light to the sample water to detect fluorescence generated by NADH or NADPH generated during the metabolism of microorganisms. can do.

In addition, by irradiating the sample water with a laser light having a high energy, it is possible to accurately detect the ultrafine particles contained in the sample water.

By incorporating ultraviolet diodes, infrared diodes, and laser diodes inside the housing, particles and microorganisms of various sizes can be accurately detected with a single probe, providing a simple and accurate diode-based turbidity system.

Hereinafter, with reference to the accompanying drawings will be described in detail for the microbial measurement probe according to a preferred embodiment of the present invention.

1 is a cross-sectional view showing the inside of the probe for turbidity measurement according to an embodiment of the present invention, Figures 2 and 3 is a partial cross-sectional view showing the inside of the probe according to another embodiment of the present invention, Figure 4 5 is a block diagram illustrating a fluorescence measurement apparatus according to an embodiment of the present invention, and FIG. 5 is a graph showing fluorescence generated when UV light is irradiated to NADH of microorganisms present in a sample water, and FIG. Figure 7 is a schematic configuration diagram of a turbidity measuring apparatus according to an embodiment of the present invention, Figure 7 is a graph measuring the amount of fluorescence detection according to the concentration of NADH by the turbidity measuring apparatus of the present invention, Figure 8 is a turbidity measuring apparatus of the present invention It is a graph measuring the fluorescence detection amount according to the concentration of microorganisms, Figure 9 is the scattered light detection amount according to the concentration of ultra-fine particles present in the sample water by the turbidity measuring device of the present invention 10 is a graph showing the influence of the laser light source applied to the formazin standard solution of the turbidity measuring device of the present invention, and FIG. It is a graph measuring the amount of scattered light detection.

1 and 4, a turbidity apparatus according to an exemplary embodiment of the present disclosure may include a main body (not shown) having an operation unit, a control unit, and a display unit, and a turbidity measurement probe 10 connected to the main body and a cable 15. )

The turbidity measurement probe 10 includes a housing 20, a light emitting unit 30 embedded in the housing 20 to emit light, a photo detector 40 for detecting light, and a light of the light emitting unit 30. It has a light emitting optical fiber 50 for transmitting to the sample water located in the lower portion of the housing 20, and a light receiving optical fiber 60 for transmitting the light or fluorescence scattered from the sample water to the photodetector (40).

The housing 20 has a cylindrical shape as a whole, and the lower part is immersed in or in contact with the sample water for turbidity measurement. The housing 20 has a structure in which the upper housing 21 and the lower housing 23 are screwed together, and an annular pad 24 for maintaining airtightness is provided between the upper housing 21 and the lower housing 23. Is mounted. The upper and lower coupling structures of the housing 20 facilitate replacement and maintenance of components in the housing 20.

The light emitting unit 30, the photodetector 40, and the light emitting and receiving optical fibers 50 and 60 are installed in the housing 20. And the optical surface for irradiating light with the sample water to measure the turbidity, that is, the bottom surface of the housing 20 is formed in a concave conical shape drawn inward. At this time, the cross section of the bottom surface of the housing 20 is formed to face each other at a 90 ° angle as shown in FIG. And the center of the bottom is formed flat is provided with a mounting portion 80 to be mounted a motor to be described later.

Through-holes are formed at one side and the other side of the bottom of the housing 20 facing each other, and the light-emitting window 25 and the light-receiving window 27 through which light is transmitted are respectively provided in the through-holes. The light emitting window 25 and the light receiving window 27 are made of glass or transparent acrylic.

The light emitting unit 30 is a light source for irradiating light to the sample water, the light emitting unit 30 constituting the turbidity measurement probe 10 of the present invention emits ultraviolet light to detect the microorganisms present in the sample water 1st lamp 31, the 2nd lamp 33 which emits a laser beam in order to detect microparticles | fine-particles, and the 3rd lamp 35 which emits near-infrared light in order to detect general turbidity.

The microparticles mean suspended solids having a size of less than 6 μm, and include ultra-fine particles having nanoscale sizes. In addition, the general turbidity refers to suspended solids that can be detected by a conventional turbidity detector, and means suspended solids from particles having a size of 6 μm or more to coarse dispersoids.

Preferably, the first lamp 31 is an ultraviolet diode emitting light having a wavelength of 330 to 350 nm, the second lamp 33 is a laser diode emitting light having a wavelength of 830 nm, and the third lamp 35 is 820. An infrared diode that emits light having a wavelength of 900 nm to 900 nm is used.

Small particles scatter more strongly in shorter wavelength bands than longer wavelength bands, and larger particles scatter more strongly in wider wavelength bands. Light sources in the 860 nm band are less sensitive to small particles, so an appropriate measurement area (particle size) must be considered. In the wavelength range of 820 to 900nm, it is easy to detect particles having a size of 6 µm or more. However, for particles having a size smaller than that, when the light is irradiated to the particles, the amount of reflected light increases and the scattered light at the 90 degree position required for turbidity detection is reduced. In order to detect ultra-fine particles of 6 μm or less, a light source having strong energy such as a laser light source is required. In particular, a laser having a wavelength band of 830 nm can detect nanoparticles having a diameter of 10 nm or less.

The first, second and third lamps 31, 33 and 35 are mounted on the printed circuit board 17 which is electrically connected with the main body together with the photodetector 40. At this time, each lamp is inserted and installed by each cylindrical lamp insertion tube 71 so that mutual light does not interfere with each other, the end of the lamp insertion tube 71 so that light can be transmitted to the light emitting optical fiber 30. One end of the light emitting optical fiber 30 is connected. In this case, a lens 73 may be inserted into the lamp insertion tube 71 between the lamp 31 and the light emitting optical fiber 51 so that light emitted from the lamp 31 may be transmitted in parallel.

As described above, the turbidity measuring probe 10 of the present invention detects microorganisms, microparticles, and general suspended solids having a relatively large particle size by using three light sources having different wavelengths, and provides accurate water quality analysis data. Can provide.

Ultraviolet light of the first lamp 31 is used to detect microorganisms. This means that the microorganisms present in water produce NADH or NADPH during metabolism. As shown in FIG. 5, NADH or NADPH utilizes the property of emitting fluorescence when ultraviolet light is irradiated. Preferably, ultraviolet light of 330 to 350 nm is used. In particular, it is preferable to irradiate with 340 nm ultraviolet light which emits 440 nm fluorescence.

Therefore, by measuring the fluorescence emitted from the microorganism at an angle of 90 ° it is possible to detect the amount of microorganisms present in the sample water.

In addition, the second lamp 33 and the third lamp 35 are used to detect suspended solids from ultrafine particles to coarse dispersoids present in the sample water in addition to the microorganisms, and the light scattered by the particles in the sample water is 90 °. By measuring at an angle to detect the amount of particles.

The light emitting optical fiber 50 is a means for transmitting the light emitted from the first, second, and third lamps to the light emitting window 25 formed on the bottom of the housing. Each lamp 31, 33, 35 It is composed of the first and second, third light emitting optical fibers 51, 53, 55 so as to transmit a light source. One end of the light emitting optical fiber 50 is connected to the lamp insertion tube 71 of the light emitting unit 30 and extends to the light emitting window 25.

Therefore, the light emitted from the light emitting part 25 is transmitted through the light emitting optical fiber 50 to the light emitting window 25 and is incident on the sample water located outside the bottom of the housing 20, and a part of the light incident on the sample water. Is scattered by suspended solids contained in the sample water. The light scattered at an angle of 90 ° is incident on the light receiving window 27 and transmitted to the photodetector 40 through the light receiving optical fiber 60.

As the light emitting and light receiving optical fibers 50 and 60 of the present invention, glass optical fibers or plastic optical fibers can be used. In particular, it is preferable to use a plastic optical fiber having excellent optical properties and processability than glass optical fiber. Plastic optical fiber has a very large ratio of core to cross section. For example, 1mm Out-dia standard fiber dia is 0.98mm, Clad dia is 0.02mm, so the ratio of core to fiber cross-sectional area is 98%. This means that the light transmission efficiency of the plastic optical fiber is very high. In addition, the plastic optical fiber has the advantages of low cost and easy installation due to excellent bending characteristics, and strong external shock.

The plastic optical fiber consists of a core made of high-purity acrylic resin (PMMA: Polymethyl methacrylate) and a thin clad layer made of special fluoropolymer (F-PMMA: Fluorine Polymethyl methacrylate). Since the refractive index of the clad is lower than that of the core, light from one end of the optical fiber causes total reflection at the connection surface of the core and the clad, and is transmitted through the core to the other end.

As described above, the light emitted from the light emitting unit 30 may be transmitted through the optical fibers 50 and 60 to reduce the volume of the probe 10 and minimize the interference of the light. In addition, since the probe 10 is not limited to the shape of the probe has a relatively different shape can be designed.

The photo detector 40 uses a photo-multiplication tube (PMT) to detect the amount of light scattered from the sample water. The optical multiplier converts the input optical signal into an electrical signal and sends it to the amplifier 110. In addition, as a photodetector 40, a photodiode may be used. The photodetector 40 is inserted into the cylindrical insertion tube 75 and installed on the printed circuit board 17 in order to prevent the light emitted from the light emitting portion from directly entering. A lens may be installed at the end 77 of the insertion tube 75.

In addition, a wiper may be disposed on the bottom surface of the housing 20 to wipe the light emitting window 25 and the light receiving window 27 when the outer surfaces of the light emitting window 25 and the light receiving window 27 are contaminated by contaminants of the sample water. 87) is installed. The wiper 87 is driven by the motor 81 mounted on the mounting portion 80 provided on the bottom surface of the housing 20. The drive shaft 83 of the motor penetrates to the outside of the housing 20, and an arm 85 having a predetermined length is coupled to an end of the drive shaft 83 protruding to the outside of the housing 20, and an end of the arm 85. The wiper 87 is installed. The wiper 87 is coupled to the frame 89 coupled by the pin 86 to the end of the arm 85 to adjust the angle formed with the arm 85, the lower portion of the frame 89 and the front end of the housing ( It consists of a blade 88 of rubber material in close contact with the bottom of the 20).

The wiper 87 rotates at least one rotation by the operation of the motor 81 to perform the cleaning operation of the light emitting window 25 and the light receiving window 27, and when stopped, the wiper 87 is disposed on the light emitting window 25 and the light receiving window 27. Stop at a location where it will not interfere so that light does not interfere with the sample water.

This is because the wiper 87 is connected to the control unit 130 through the wiper driving circuit 170 so that a certain time has elapsed or the light emitting window 25 or the light receiving window 27 is dirty and abnormal turbidity is measured. The cleaning operation is performed by the control. Therefore, the surfaces of the light emitting window 25 and the light receiving window 27 can be kept clean at all times, thereby enabling accurate turbidity measurement.

In addition, a power source for driving the light emitting unit 30 and the motor 81 may be supplied using a battery (not shown) built in the housing 20 or through a cable 15 connected to the main body.

On the other hand, the turbidity measurement probe according to another embodiment of the present invention, as shown in Figure 3 is formed a bent portion of the bent at a right angle to the bottom surface of the housing 20, the light emitting optical fiber 50 on one side of the bent portion A light emitting window 25 through which light emitted from an end of the light incident side is incident is provided, and a light receiving window 27 through which scattered light is incident is provided on the other side of the bent portion opposite to the light emitting window 25.

And the turbidity measurement probe according to another embodiment of the present invention, as shown in Figure 2 the end of the light-emitting and light-receiving optical fiber 50, 60 penetrates the bottom of the housing 20 of the housing 20 It may have a structure that is installed to be exposed to the outside.

And the light emitting unit 30 is applied to the turbidity measurement probe 10 of the present invention, unlike shown, may be composed of a first lamp alone to detect microorganisms, or a second lamp alone to detect particulates. . In addition, the light emitting unit may be configured by combining two lamps among the first lamp and the second and third lamps.

Referring to the operation of the turbidity measurement probe 10 of the present invention.

First, when power is applied to the first lamp 31 of the light emitting unit 30 while the lower portion of the housing 20 is locked to the sample water to measure turbidity, 340 nm through the first light emitting optical fiber 51. Ultraviolet light having a wavelength of about 70 is emitted through the light emitting window 25 and is incident on the number of samples located under the housing 20. Ultraviolet light incident on the sample water is excited by NADH or NADPH of the microorganisms present in the sample water and emitted in 450 nm fluorescence. At this time, the emitted fluorescence is incident to the light receiving window 27 and transmitted to the photodetector through the light receiving optical fiber 60.

When power is applied to the second lamp 33 or the third lamp 35 of the light emitting unit 30, light is incident on the sample water through the second light emitting optical fiber 53 or the third light emitting optical fiber 55. do. The light incident on the sample water is scattered by the particles present in the sample water, and the light incident on the light receiving window 27 is scattered at a 90 ° angle among the light detectors through the light receiving optical fiber 60. Is delivered.

The main body connected to the probe 10 and the cable 15 to receive an electrical signal output through the photodetector 40 has a substantially rectangular shape, and a display unit 160 for electronically displaying a screen is formed on a front surface thereof. On one side of the display unit 160, an operation unit 140 for various settings and manipulations is provided, and a control unit 130 is located therein. And a power supply unit (not shown) for supplying power to the main body is further provided. The power supply unit may use a battery built in the inside of the main body, or may use a power source introduced through a wire from the outside.

In addition, a conventional amplifier 110 and an A / D converter 120 are connected to the main body, thereby amplifying an electrical signal from the photodetector 40, converting the electrical signal into a digital signal, and inputting the digital signal to the controller 130.

The controller 130 calculates and processes the input signal and outputs an electrical signal to the display unit 160 through the display driver circuit 150. In addition, the controller 130 controls the lamps 31, 33, 35 of the light emitting unit 30 to be selectively driven by the manipulation unit 140. Then, the electric signal is output to the wiper driving circuit 170 to drive the motor 81.

The operation unit 140 may apply power to the turbidity measuring apparatus of the present invention, operate various devices, and selectively drive any one of the lamps of the light emitting unit 30. The operation unit 140 includes a mode selection button, an up button, a down button, a set button, a power button, and the like.

The display unit 160 displays the measured turbidity at a predetermined value or displays the currently set mode.

Hereinafter, the characteristics of the turbidity measurement probe of the present invention will be described through Examples and Experimental Examples. However, the following examples are intended to illustrate the present invention in detail, and the scope of the present invention is not limited to the following examples.

(Example)

The turbidity sensor is a light source of the light emitting part, and an ultraviolet diode (SEOUL OPTO DEVICE Co., S8D28, 340 nm) for detecting microorganisms and a laser diode (Hamamatsu Co., Japan, L8414-41, 830 nm) for detecting ultrafine particles (nanoparticles) ) And a near-infrared diode (WI3311-H, 860 nm, Wonsemiconductor Co., Korea) was used for the detection of general turbidity.

In addition, a 2 mm diameter plastic optical fiber (Mitsubishi Co., Japan, SH-8001) was used to transmit the light emitted from the light emitting part to the sample water located at the bottom of the probe. A photomultiplier tube (PMT, H5783, Hamamatsu Co., Japan) was used as the photodetector.

Figure 5 shows the experimental configuration of the turbidity sensor, the light irradiated from the light emitting part is incident on the sample contact surface of the lower end of the probe through the light emitting optical fiber, and the scattered light generated by suspended matter such as particulates in the sample water and The fluorescence generated by the microorganisms was transmitted to the photomultiplier tube by the optical fiber for receiving light at a 90 ° angle. In the photomultiplier, the transmitted scattered light and fluorescence were amplified and displayed as voltage.

Experimental Example 1 Microbial Detection Experiment

First, in order to detect microorganisms in water, NADH produced during the metabolic process of microorganisms was detected by a turbidity measurement apparatus using the probe of the present invention. Β-nicotinamide adenine dinucleotide (NADH) was purchased from Sigma for the detection experiment. The sample water was prepared by diluting the NADH in tertiary distilled water, and irradiated with ultraviolet light having a wavelength band of 340 nm. In addition, using a fluorescence spectrophotometer (F-4500, Hitachi Co., Japan) was shown in Figure 7 by comparing the detection performance for NADH.

Referring to FIG. 7, as the concentration of NADH in the sample water increased from 0 to 0.5 mM, the amount of fluorescence emission also increased, and the difference in detection signal was about 2 V, which showed very high sensitivity. And when compared with the results measured by the fluorescence spectrophotometer showed a high linearity.

Next, E. coli DH5α and Bacillus subtilis type NCT 3601 were used as microorganisms for actual microbial detection experiments. Incubate E. coli DH5α and Bacillus subtilis type NCT 3601 for 24 hours using a shaker, centrifuge, wash with distilled water, re-centrifuge, take only microorganisms, and dilute microorganisms in tertiary distilled water. Prepared. In the microbial detection experiment, E. coli and Bacillus were detected by varying the number of individuals from 0 to 2.5 × 10 ³ CFU, respectively, and the results are shown in FIG. 8.

Referring to FIG. 8, as the number of microorganisms increased, the signal detected by the turbidity measuring device also increased linearly. In the detection of microorganisms, Bacillus showed higher sensitivity than that of E. coli, and this phenomenon appears to be due to the production of a large amount of NADH or NADPH.

2. Experimental Example 2: Ultrafine Particle Detection Experiment

Fe ₃ O ₄ with a particle size distribution of 10 to 20 nm for the detection of ultrafine particles Nanoparticles were synthesized and detection experiments were performed.

Fe ₃ O ₄ In order to synthesize nanoparticles, ferric ammonium sulfate and iron (III) chloride were mixed at a 1: 2 molar ratio, followed by stirring at 80 ° C. and adding 28% ammonium aqueous solution for 30 minutes to synthesize nanoparticles. At this time, the pH of the reaction solution was maintained at 10, and the reaction formula is as follows.

^{Fe 2 + + 2Fe 3 + +} 8OH - → Fe 3 O 4 + 4H ₂ O

After the reaction, the mixture was washed with distilled water and ethanol, and the nanoparticles and the solution were separated using a magnet of 6 × 10 3 Gauss, followed by vacuum drying at 70 ° C. Fe ₃ O ₄ in the tertiary distilled water filtered using a cellulose membrane having a pore size of 0.45 ㎛ as the sample water The nanoparticles were diluted and irradiated with laser light having a wavelength band of 830 nm.

9 shows Fe ₃ O ₄ from 0 to 0.5 ppm This is the result of measuring the scattered light by laser diode by changing the concentration of nanoparticles. As shown in FIG. 9, it was confirmed that the detection of ultrafine particles in water quality using a laser light source had a high linearity and a significance level of 1.0 × 10 ⁻⁴ or less.

In order to investigate the influence of the laser light source on the general turbidity solution, laser light is irradiated to the sample water diluted with 4000 NTU standard forazine solution (HACH Co., USA) in distilled water and the results are shown in FIG. Indicated.

As a result, when the turbidity of the sample water was changed to a concentration of 0 to 10 NTU as shown in FIG. 10, the same signal as that of the ultra-fine particle concentration of 0 ppm in FIG. 9 was detected. It is not affected and shows high selectivity in ultrafine particle detection.

3. Experimental Example

In order to detect general turbidity, turbidity detection experiments were carried out using the above-described forazine standard solution. To this end, the near-infrared LED light having a wavelength band of 860 nm was irradiated to the sample water diluted with 4000 NTU standard forazine solution (HACH Co., USA) in tertiary distilled water, and the results are shown in FIG. 11.

Referring to FIG. 11, when the change in turbidity of the sample water was changed from 0 to 1.0 NTU using a forazine standard solution, high linearity of 99% or more was observed at low concentrations of turbidity. And even more than 0.3 NTU showed high linearity, but the sensitivity of the sensor was slightly lower. This phenomenon is caused by turbidity in solution as the concentration of formazine increases, so that incident light irradiated from the light source hits the suspended material and is scattered, and the scattered light is disturbed by the suspended material in the process of reaching the detector. The sensitivity seems to be lowered.

From the above results, the turbidity measuring apparatus using the turbidity measuring probe of the present invention can accurately detect not only general turbidity but also ultrafine particles or microorganisms, so that turbidity measurement can be performed precisely. This is realized by the turbidity measurement probe of the present invention in which the detection of each particle and microorganism according to the size of suspended solids present in water is adopted by adopting different light sources for generating scattered light.

Although the present invention has been described with reference to one embodiment shown in the drawings, this is merely exemplary, and it will be understood by those skilled in the art that various modifications and equivalent embodiments thereof are possible.

Therefore, the true scope of protection of the present invention should be defined only by the appended claims.

1 is a cross-sectional view showing the inside of the turbidity measurement probe according to an embodiment of the present invention,

2 and 3 is a partial cross-sectional view showing the inside of the probe according to another embodiment of the present invention,

Figure 4 is a block diagram of a turbidity measuring device according to an embodiment of the present invention,

5 is a graph showing a three-dimensional spectrum of fluorescence generated when irradiating ultraviolet light to the NADH of the microorganisms present in the sample water,

6 is a schematic configuration diagram of a turbidity measuring apparatus according to an embodiment of the present invention,

7 is a graph measuring the amount of fluorescence detection according to the concentration of NADH by the turbidity measuring device of the present invention,

8 is a graph measuring the amount of fluorescence detection according to the concentration of the microorganism by the turbidity measuring device of the present invention,

9 is a graph measuring the amount of scattered light detection according to the concentration of ultrafine particles present in the sample water by the turbidity measuring device of the present invention,

FIG. 10 is a graph showing the effect of the laser light source applied to the turbidity measuring device of the present invention on a formazin standard solution.

11 is a graph measuring the amount of scattered light detected according to the concentration in the formazin standard solution by the turbidity measuring apparatus of the present invention.

<Description of the symbols for the main parts of the drawings>

10: probe 20: housing

25: light emitting window 27: light receiving window

30: light emitting unit 31: first lamp

33: second lamp 35: third lamp

40: photodetector 50: light emitting optical fiber

60: light receiving optical fiber 81: motor

87: wiper 130: control unit

140: operation unit 160: display unit

Claims (8)

delete delete delete delete delete A cylindrical housing formed such that left and right sides of the bottom contacting the sample water are perpendicular to each other with respect to the center of the bottom; A light emitting window formed on one side of a bottom surface of the housing and configured to transmit light; A light receiving window formed on the other side of the bottom of the housing and configured to transmit light at right angles to the optical axis emitted from the light emitting window; A light emitting unit embedded in an upper portion of the housing and having a first lamp for emitting ultraviolet light for detecting microorganisms contained in the sample water; A photodetector embedded in an upper portion of the housing and detecting fluorescence generated in the sample water by light emitted from the light emitting part to the outside of the housing; A light emitting optical fiber which has one end connected to the light emitting part and the other end extending to the light emitting window to transmit the light emitted from the light emitting part to the sample water located outside the housing; And a light receiving optical fiber having one end connected to the photodetector and the other end extending to the light receiving window to transmit light incident from the sample water into the housing through the light receiving window to the photodetector. Probe for measuring microorganisms. [7] The apparatus of claim 6, further comprising a drive motor installed inside the housing, and a wiper installed outside the bottom of the housing to be driven by the drive motor to wipe the light emitting window and the light receiving window. Probe for measuring microorganisms. delete

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