KR20220160380A - Apparatus for controlling temperature of pcr device using thermoelectric element - Google Patents

Abstract

A temperature control device for a gene amplification device according to one aspect of the present invention includes: a frame part; a reaction vessel located inside the frame part, accommodating a sample therein and transferring external heat to the sample; a temperature control part located on one side of the frame part and moving from the lower side of the reaction vessel to control the temperature of the sample; a driving part connected to the temperature control part to move the temperature control part; and a movement control part located on the other side of the frame part and controlling the movement of the temperature control part by the driving part. An object of the present invention is to provide the temperature control device for the gene amplification device having a structure capable of reducing the time required for temperature change in each step during a PCR process.

Description

Temperature control device of gene amplification device {APPARATUS FOR CONTROLLING TEMPERATURE OF PCR DEVICE USING THERMOELECTRIC ELEMENT}

The present invention relates to a temperature control device for a gene amplification device.

Polymerase chain reaction (PCR) is a molecular biological technique that copies and amplifies a desired part of DNA. The polymerase chain reaction generally goes through three steps: DNA denaturation, primer binding (annealing), and DNA synthesis (elongation). The experiment is run by repeating the above three steps, each step being run at a different temperature.

DNA denaturation is a process of separating DNA strands and is mainly performed at 92℃~95℃. The primer binding process is a step in which a DNA primer binds to DNA, and is mainly performed at 50~60℃. The DNA synthesis process is a stage in which DNA replication occurs by DNA polymerase, and is mainly performed at 72℃.

Conventionally, when a current is passed to reach a set temperature, a Peltier element using a Peltier effect in which one side emits heat and the other side absorbs heat has been utilized. However, when a single Peltier device is used, it takes a certain amount of time when the temperature changes for each step, which not only increases the amplification time of all genes, but also has a problem in that the yield of genes decreases.

In order to solve the above problem, an object of the present invention is to provide a temperature control device of a gene amplification device having a structure capable of reducing the time required when the temperature is changed in each step during the PCR process.

A temperature control device for a gene amplification device according to an aspect of the present invention includes a frame part, a reaction container located inside the frame part, accommodating a sample therein and transferring external heat to the sample, and located on one side of the frame part. , A temperature control unit that moves from the lower side of the reaction vessel and controls the temperature of the sample, a drive unit connected to the temperature control unit and moving the temperature control unit, and located on the other side of the frame unit, the temperature control unit by the drive unit A movement control unit for controlling the movement of the control unit may be included.

The frame unit may include a lower frame and an insulating frame coupled to the lower frame to prevent heat transfer to the outside.

The temperature controller may include a plurality of thermoelectric elements for heating or cooling the reaction vessel.

The thermoelectric elements may be adjusted to different set temperatures, respectively.

The temperature controller may further include a cooling block contacting one surface of the thermoelectric element.

The temperature controller may further include a cooling fan contacting the other surface of the cooling block.

The movement control unit is coupled to the lower frame, is connected to an input module to which an input signal is input, is connected to the input module, and is connected to a control module that sends a control signal and an output signal based on the input signal and the control module, An output module outputting the output signal may be included.

The driving unit may include a driving motor connected to the control module and driven according to the control signal, and a thermoelectric element moving module connected to the driving motor to move the thermoelectric element.

The thermoelectric element movement module is coupled to a first gear connected to the driving motor, a guide rail coupled to the lower frame, and coupled to a guide rail for guiding a movement path of the thermoelectric element, coupled to the guide rail, and connected to the thermoelectric element to form the guide It may include a guide block sliding on the rail and a second gear coupled to the guide block and meshed with the first gear.

The reaction vessel may include a well block having at least one groove formed thereon and a tube containing the sample inserted into the groove.

In the temperature control device of the gene amplification device according to one aspect of the present invention, a height adjustment column coupled to the lower frame and extending in a first direction, and a second height adjustment column coupled to the height adjustment column and intersecting the first direction The head portion may further include a top plate protruding in a direction and adjustable in height in the first direction, and a protruding part coupled to the top plate and formed to correspond to the groove of the well block.

As described above, the temperature control device of the gene amplification device according to one aspect of the present invention can rapidly change the temperature at each step without time delay.

Accordingly, the temperature of the reaction sample can be stably controlled, the overall PCR process time can be shortened, and the gene production yield can be improved through accurate temperature control.

1 is a side perspective view of a temperature control device of a gene amplification device according to an embodiment of the present invention.
FIG. 2 is a schematic cross-sectional view taken along line II-II′ of FIG. 1 .
3 is a diagram schematically illustrating a driving unit and a movement control unit according to an embodiment of the present invention.
Figure 4 is a plan view of Figure 3;
5 is a temperature change graph of a polymerase chain reaction.
6 is a flowchart of a step of moving a thermoelectric element according to an embodiment of the present invention.
7 is a side perspective view of a temperature control device of a gene amplification device having a plurality of zones according to an embodiment of the present invention.

Since the present invention can apply various transformations and have various embodiments, specific embodiments will be exemplified and described in detail in the detailed description. However, it should be understood that this is not intended to limit the present invention to specific embodiments, and includes all transformations, equivalents, and substitutes included in the spirit and scope of the present invention.

Terms used in the present invention are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In the present invention, terms such as 'include' or 'having' are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. At this time, it should be noted that in the accompanying drawings, the same components are indicated by the same reference numerals as much as possible. In addition, detailed descriptions of well-known functions and configurations that may obscure the gist of the present invention will be omitted. For the same reason, in the accompanying drawings, some components are exaggerated, omitted, or schematically illustrated.

Hereinafter, a temperature control device of a gene amplification device according to an embodiment of the present invention will be described.

1 is a side perspective view of a temperature control device of a gene amplification device according to an embodiment of the present invention. FIG. 2 is a schematic cross-sectional view taken along line II-II′ of FIG. 1 . 3 is a side perspective view of a driving unit and a movement control unit according to an embodiment of the present invention. Figure 4 is a plan view of Figure 3;

1 to 4, the temperature control device 10 of the gene amplification device according to an embodiment of the present invention includes a frame part 100, a reaction container 300, a movement control part 400, and a head part 500. ), a temperature control unit 700 and a driving unit 900 may be included. Here, those skilled in the art related to this embodiment know that other general-purpose components other than those shown in FIGS. 1 to 4 may be included in the temperature control device 10 of the gene amplification device. You will understand when you grow up.

The frame unit 100 according to this embodiment may include a lower frame 110, an insulating frame 120, a reaction vessel inlet 130, a reaction vessel inlet cover 140, and an upper cover 150. In addition, those skilled in the art can understand that other general-purpose components may be included in the frame unit 100.

1 to 3, the lower frame 110 is coupled to the lower portion of the insulating frame 120 to be described later, and the movement control unit 400, the temperature control unit 700, and the driving unit 900 are included therein. can The lower frame 110 may be hollow and have a hexahedral shape having a plane with one side of an edge protruding.

An input module 410 and an output module 420 to be described below may be positioned outside the protruding portion 160 of the lower frame 110 . Inside the protruding portion 160 of the lower frame 110, a control module 430 and a power supply unit 440, which will be described later, may be located. A temperature control unit 700 and a driving unit 900 may be located inside the lower frame 110 .

The lower frame 110 may be made of materials such as plastic and steel having sufficient rigidity to store the movement control unit 400, the temperature control unit 700, and the driving unit 900 accommodated therein. have. However, the shape and internal structure of the lower frame 110 are not limited thereto and may be changed within a range that can be employed by those skilled in the art.

Referring to FIG. 1 , the heat insulation frame 120 is coupled to the upper portion of the lower frame 110 to prevent heat transfer to the outside. The insulating frame 120 may be disposed along the edge of the lower frame 110 to form a space in which the reaction container 300 can be located. The heat insulating frame 120 is coupled to the lower frame 110 without a gap to maintain a constant temperature of the inner space where the reaction vessel 300 described later is located. The insulating frame 120 may be made of insulating glass so that the temperature of the internal space is kept constant and the internal space can be viewed from the outside. However, the shape and material of the insulating frame 120 are not limited thereto and may have various shapes and materials.

The reaction vessel inlet 130 is formed in the heat insulation frame 120 and may be an inlet for inserting the reaction vessel 300 into an inner space surrounded by the heat insulation frame 120 . The reaction vessel inlet 130 may be formed as a square hole on the side of the heat insulating frame 120 to the extent that the reaction vessel 300 can enter. However, the shape and position of the reaction container inlet 130 is not limited thereto and may have various shapes and positions.

The reaction vessel inlet cover 140 may serve to cover the reaction vessel inlet 130 in order to keep the temperature of the inner space constant. The reaction vessel inlet cover 140 may be formed in a rectangular plate shape corresponding to the reaction vessel inlet 130 . The reaction vessel inlet cover 140 may be made of the same material as the heat insulating frame 120 . The reaction vessel inlet cover 140 may be coupled to a corner of the reaction vessel inlet using a hinge so as to be easily opened and closed. However, the shape and material of the reaction container inlet cover 140 is not limited thereto and may have various shapes and materials.

The upper cover 150 may serve to cover the open upper portion of the heat insulating frame 120 . The upper cover 150 may be formed in the form of a rectangular plate corresponding to a rectangular plane when the insulating frame 120 is viewed from above. The upper cover 150 may be coupled to the heat insulating frame 120 without a gap. Like the insulating frame 120, the upper cover 150 may be made of insulating glass to keep the temperature of the interior space constant. The upper cover 150 may serve as an upper passage leading from the outside to the inner space in addition to the reaction vessel inlet cover 140 . However, the shape and material of the upper cover 150 is not limited thereto and may be changed within the range that can be employed by those skilled in the art.

The reaction vessel 300 is located inside the frame unit 100, and can accommodate a sample therein and transfer external heat to the sample. The reaction vessel 300 may include a well block 310 in which at least one groove is formed and a tube 320 containing the sample inserted into the groove. However, the configuration of the reaction container 300 is not limited thereto, and those skilled in the art can understand that other general-purpose components may be further included.

The well block 310 may have at least one groove formed therein to accommodate a tube 320 to be described later. The well block 310 may have a rectangular block shape including grooves formed of a plurality of rows and columns. The well block 310 may be made of a material such as thermally conductive plastic to transfer heat from the temperature controller 700 located at the bottom to the sample. However, the shape and material of the well block 310 are not limited thereto and may have various shapes and materials.

The tube 320 is inserted into the groove of the well block 310 and may contain a sample. The tube 320 may have a cylindrical shape with curved corners at the bottom and an open top. The tube 320 may be made of glass or plastic so that the state of the contained sample can be checked from the outside. In addition, the tube 320 may have various shapes and materials capable of containing a sample.

Referring to FIGS. 3 and 4 , the movement control unit 400 is located on the other side of the frame unit 100 and can control the movement of the temperature control unit 700 to be described later by the driving unit 900 to be described later. The movement controller 400 may include an input module 410, an output module 420, a control module 430, a power supply 440, and a temperature sensor 450. However, those skilled in the art can understand that other general-purpose components other than those shown in FIGS. 3 and 4 may be further included in the movement control unit 400 .

Referring to FIGS. 1 and 3 , the input module 410 is coupled to the lower frame 110 so that an input signal can be input. The input module 410 may have any structure into which an input signal may be input. For example, a numeric keypad for setting the temperature of a sample may be utilized, and a touch pad may be utilized. An input signal such as a set temperature and a duration may be input to the input module 410, and the input signal may be transmitted to a control module described later. In addition, the input module 410 of various shapes and structures may be possible.

5 is a temperature change graph of a polymerase chain reaction (PCR). The polymerase chain reaction generally goes through three steps: DNA denaturation, primer binding (annealing), and DNA synthesis (elongation). As shown in FIG. 5, it is possible to selectively amplify only a specific DNA fragment in a small amount of DNA solution by repeatedly executing the three steps. In general, 94 ℃ for the DNA denaturation step, 72 ℃ for the primer binding step, and 50 ~ 60 ℃ for the DNA synthesis step are known to be suitable temperatures.

The control module 430 is connected to the input module 410 and can send a control signal and an output signal based on the input signal. The control module 430 may determine a control signal based on the input signal and the temperature measured by the temperature sensor 450 to be described later. For example, the control module 430 controls the temperature of the DNA denaturation step to be 94 ° C, but when the temperature of the sample is 60 ° C, the thermoelectric element set to 94 ° C among the thermoelectric elements described below is located at the bottom of the reaction vessel 300. control signals can be determined.

The output module 420 may be connected to the control module 430 and output an output signal. The output module 420 may have any structure capable of outputting an output signal. For example, the output module 420 is configured in the form of a liquid crystal display (LCD) plate, and output signals such as the temperature of the current sample, the set temperature, and the currently corresponding process step can be output to the LCD plate. In addition, the output module 420 may have various shapes and materials.

The power supply unit 440 may be coupled to the lower frame 110 to supply power to the movement control unit 400 , the driving unit 900 and the temperature control unit 700 . Wires may be connected between the power supply unit 440, the movement control unit 400, the driving unit 900, and the temperature control unit 700. The power supply unit 440 may have any configuration capable of supplying power to the movement control unit 400, the driving unit 900, and the temperature control unit 700.

The temperature sensor 450 may be coupled to the reaction container 300 to measure the temperature of the sample (see FIG. 1). The temperature sensor 450 may be attached to one side of the well block 310 to measure the temperature of the sample contained in the tube 320 . The temperature sensor 450 is connected to the control module 430 and provides the temperature of the current sample to help determine the control signal. A non-contact temperature sensor such as an infrared sensor may be used as the temperature sensor 450 . However, the location and type of the temperature sensor 450 is not limited thereto and may have various locations and types.

Referring to FIG. 1 , the head part 500 is coupled to the lower frame 110 so that a sample or a reagent may be injected into the reaction vessel 300 . The head part 500 may include a height adjustment pillar 510, an upper plate 520, and a protruding part 530. However, those of ordinary skill in the art related to the present embodiment may understand that other general-purpose components other than those shown in FIG. 1 may be included in the head unit 500 .

The height adjustment pillar 510 is coupled to the lower frame 110 and extends in a first direction to serve as a pillar for fixing an upper plate 520 to be described later. Here, the first direction may be a direction perpendicular to the top of the lower frame 110 . The height adjustment pillar 510 may have a hollow square pillar shape so that a part of an upper plate 520 to be described later can be inserted therein. The height adjustment pillar 510 may be made of a material such as steel having sufficient rigidity to allow the upper plate 520 to be fixed. However, the shape and material of the height adjustment pillar 510 is not limited thereto and may have various shapes and materials.

The top plate 520 is coupled to the height adjustment pillar 510, protrudes in a second direction crossing the first direction, and may be height-adjustable in the first direction. Here, the second direction perpendicularly intersects the first direction and may be a horizontal direction on the top of the lower frame 110 . The upper plate 520 may be in the form of a square plate having a protruding part that can be fitted into the empty space of the height adjustment pillar 510 . The protruding part of the top plate 520 is inserted into the height adjustment pillar 510 and can slide vertically up and down. However, the shape and coupling relationship of the upper plate 520 is not limited thereto and may have various shapes and positions.

The protruding part 530 is coupled to the upper plate 520 and is formed to correspond to the groove of the well block 310 to insert a sample or inject a reagent. A plurality of protruding parts 530 may be formed on the surface of the upper plate 520 facing the lower frame 110 . The protruding part 530 may have a diameter smaller than that of the tube 320 so as to be inserted into the tube 320 when the protruding part 530 moves downward along with the upper plate 520 . However, the shape of the protruding part 530 is not limited thereto and may have various shapes.

Referring to FIGS. 2 to 4 , the temperature control unit 700 is located on one side of the frame unit 100 and moves from the lower side of the reaction vessel 300 to control the temperature of the sample. The temperature controller 700 may include a thermoelectric element 710, a cooling block 720, a cooling fan 730, an air duct 740, and a heat conduction plate 750. However, those skilled in the art can understand that other general-purpose components may be further included in addition to the components shown in FIGS. 2 to 4 .

The thermoelectric element 710 may heat or cool the reaction vessel 300 by being coupled by a guide block 930b to be described later and a connection frame 940 to be described later. The thermoelectric element 710 may be a Peltier element that absorbs heat on one side and emits heat on the other side when a current is applied. A plurality of thermoelectric elements 710 may be formed and adjusted to a set temperature for each step of a polymerase chain reaction (PCR). The thermoelectric element 710 is set to a different set temperature, so that the thermoelectric element 710 set to the set temperature of the corresponding step reacts without the need to change the temperature of the thermoelectric element 710 when proceeding to the next step in the polymerase chain reaction. The temperature can be controlled by placing it in the lower part of the container 300 . The time required for temperature control of the thermoelectric element 710 may be reduced by increasing the number of the thermoelectric elements 710 and locating appropriate thermoelectric elements in each step (see FIG. 6 ). However, the number and connection relationship of the thermoelectric elements 710 may be changed as needed.

The cooling block 720 may contact one surface of the thermoelectric element 710 to prevent the thermoelectric element 710 from overheating. The cooling block 720 transfers heat generated from the thermoelectric element 710 to the outside so that rapid cooling can be achieved. The cooling block 720 may be made of aluminum or copper having high thermal conductivity. The cooling block 720 can expect a cooling effect due to a natural convection effect. The cooling block 720 may have a rectangular plate shape having a plurality of protruding parts in order to maximize a cooling effect by increasing a surface area (see FIG. 2 ). In addition to the illustrated structure, the cooling block 720 may have a structure such as a heat pipe. However, the shape and material of the cooling block 720 are not limited thereto and may have various shapes and materials.

The cooling fan 730 may prevent the thermoelectric element 710 from overheating by blowing air in contact with the other surface of the cooling block. The cooling fan 730 may allow outside air to flow through an air duct 740 to be described later. The cooling fan 730 may have various fan shapes capable of flowing air.

The air duct 740 is coupled to the cooling fan 730 and may serve as a passage for convection with outside air. The air duct 740 may have a cylindrical shape with both ends penetrating and vertically bent. One end is coupled to the cooling fan 730 without a gap and the other end to the lower frame 110. It may be formed in the form of a hole. However, the shape and position of the air duct 740 is not limited thereto and may have various shapes and positions.

The heat conduction plate 750 may be coupled to an upper end of the lower frame 110 to transfer heat generated from the thermoelectric element 710 to the reaction vessel 300 . The reaction vessel 300 may be placed on top of the heat conduction plate 750 . The heat conduction plate 750 may have a rectangular plate shape larger than the plane of the reaction vessel 300 . The heat conduction plate 750 may be made of aluminum or copper having high thermal conductivity in order to provide uniform heat to the reaction container 300 . However, the shape and material of the heat conduction plate 750 are not limited thereto and may be changed within a range that can be employed by those skilled in the art.

The driving unit 900 may be connected to the temperature controller 700 to move the temperature controller 700 . The driving unit 900 may include a driving motor 910 , a driving shaft 920 , a thermoelectric element movement module 930 and a connection frame 940 . However, those skilled in the art can understand that other general-purpose components may be further included in addition to the components shown in FIGS. 3 and 4 .

The driving motor 910 may be connected to the control module 430 and driven according to a control signal. The driving motor 910 may serve to provide power for movement of the thermoelectric element 710 . A driving shaft 920 is coupled to the driving motor 910 to generate torque using the driving shaft 920 as a rotational shaft. The drive motor 910 may be any type of motor for providing power.

The thermoelectric element movement module 930 may be connected to the driving motor 910 to move the thermoelectric element (see FIG. 3 ). The thermoelectric element movement module 930 may include a guide rail 930a, a guide block 930b, a first gear 930c, and a second gear 930d. However, those skilled in the art can understand that other general-purpose components may be further included in addition to the components shown in FIG. 3 .

The guide rail 930a is coupled to the lower frame 110 and may guide a movement path of the thermoelectric element 710 . The guide rail 930a may be a linear member along the movement path of the thermoelectric element 710 or may have a protruding portion formed at an upper end for coupling with a guide block 930b to be described later. The guide rail 930a may utilize an LM guide (linear motion). However, the shape of the guide rail 930a is not limited thereto and may have various shapes.

The guide block 930b is coupled to the guide rail 930a and connected to the thermoelectric element 710 to slide the guide rail 930a. The guide block 930b may have a block shape having a sufficient length so that the plurality of thermoelectric elements 710 may be connected at regular intervals. A guide block 930b may have a groove formed therein so as to be fitted with the protruding portion of the guide rail 930a. An LM guide block may be used as the guide block 930b. However, the shape of the guide block 930b is not limited thereto and may have various shapes.

The first gear 930c may be connected to the drive motor 910 to transmit power of the drive motor 910 . The first gear 930c may have a shape in which protrusions (gear teeth) are formed at regular intervals on a disk-shaped rotating body. The center of the first gear 930c is connected to the drive shaft 920 so that the drive shaft 920 can be used as a rotation shaft to perform a rotational motion. The first gear 930c may function as a pinion gear among a rack gear and a pinion gear by meshing with a second gear 930d to be described later. However, the shape of the first gear 930c is not limited thereto, and any shape capable of transmitting power is possible.

The second gear 930d is coupled to the guide block 930b and meshes with the first gear 930c to convert the rotational motion of the first gear 930c into linear motion. The second gear 930d is coupled to the upper portion of the guide block 930b and may have a rectangular shape in which one side is longer than the other side, and protrusions (teeth of the gear) are formed at regular intervals. The second gear 930d may function as a pinion gear among rack gears and pinion gears by meshing with the first gear 930c. The second gear 930d may mesh with the first gear 930c to move the thermoelectric element 710 in a direction parallel to the guide rail 930a. However, the shape of the second gear 930d is not limited thereto, and any shape capable of converting rotational motion into linear motion is possible.

The connection frame 940 may serve to connect the thermoelectric element 710 and the guide block 930b by being coupled to the guide block 930b. The connection frame 940 may have a long rod shape between the thermoelectric element 710 and the guide block 930b. The connection frame 940 may be made of a material having sufficient rigidity, such as steel, to support the weight of the thermoelectric element 710 and to be fixed even when the thermoelectric element 710 moves. However, the shape and material of the connection frame 940 is not limited thereto and may have various shapes and materials.

6 is a flowchart of a step of moving a thermoelectric element according to an embodiment of the present invention.

Referring to FIG. 6 , the thermoelectric element 710 may be moved to an appropriate position according to the set temperature for each step of the polymerase chain reaction (PCR). For example, in FIG. 6, the right thermoelectric element 710a is 94 ° C (appropriate temperature for DNA denaturation process), the middle thermoelectric element 710b (appropriate temperature for primer bonding process) is 60 ° C, and the left thermoelectric element 710c ( Optimum temperature of the DNA synthesis process) can be set to 72 ℃. Later, when the DNA denaturation process is followed by the primer bonding process, the thermoelectric element 710 adjusted to 72 ° C. can be placed under the heat conduction plate 750 as shown in FIG. 6 without the need to lower the thermoelectric element 710 from 94 ° C. to 72 ° C. can Accordingly, when each step is performed, the temperature of the thermoelectric element 710 may be immediately changed to a set temperature instead of being gradually changed. In this way, the polymerase chain reaction may be performed, and the time required according to the temperature change of the thermoelectric element 710 may be shortened. In addition, the gene production yield can be improved through precise temperature control.

7 is a side perspective view of a temperature control device 10 of a gene amplification device having a plurality of zones according to an embodiment of the present invention.

Referring to FIG. 7 , the inner space of the insulating frame 120 may be partitioned into a plurality of zones 200 . For example, as shown in FIG. 7 , the inner space of the insulating frame 120 may be divided into a first zone 210 , a second zone 220 and a third zone 230 . Each zone 200 may have one movement control unit 400, a head unit 500, a reaction vessel 300, a temperature control unit 700, and a driving unit 900. Due to this, polymerase chain reaction (PCR) under different conditions for each zone is possible.

Although one embodiment of the present invention has been described above, those skilled in the art can add, change, delete, or add components within the scope not departing from the spirit of the present invention described in the claims. The present invention can be variously modified and changed by the like, and this will also be said to be included within the scope of the present invention.

10 Temperature control device of gene amplification device
100 frame part
200 zone
300 reaction vessel
400 movement control unit
500 head
700 temperature controller
900 driving unit

Claims (11)

frame part;
a reaction vessel located inside the frame unit, accommodating a sample therein and transferring external heat to the sample;
a temperature control unit located at one side of the frame unit and moving from the lower side of the reaction vessel to control the temperature of the sample;
a driving unit connected to the temperature controller and moving the temperature controller; and
A temperature control device of a gene amplification device comprising a movement control unit located on the other side of the frame unit and controlling movement of the temperature controller by the driving unit. According to claim 1,
the frame part,
lower frame and
A temperature control device of a gene amplification device comprising an insulating frame coupled to the lower frame to prevent heat transfer to the outside. According to claim 2,
The temperature controller,
A temperature control device for a gene amplification device comprising a plurality of thermoelectric elements for heating or cooling the reaction vessel. According to claim 3,
The thermoelectric element is a temperature control device of a gene amplification device set to a different set temperature. According to claim 4,
The temperature controller,
The temperature control device of the gene amplification device further comprising a cooling block in contact with one surface of the thermoelectric element. According to claim 5,
The temperature controller,
The temperature control device of the gene amplification device further comprising a cooling fan in contact with the other surface of the cooling block. According to claim 4,
The movement control unit,
an input module coupled to the lower frame and inputting an input signal;
a control module connected to the input module and sending a control signal and an output signal based on the input signal; and
A temperature control device of a gene amplifying device comprising an output module connected to the control module and outputting the output signal. According to claim 7,
the driving unit,
A drive motor connected to the control module and driven according to the control signal; and
A temperature control device of a gene amplification device comprising a thermoelectric element movement module connected to the driving motor and moving the thermoelectric element. According to claim 8,
The thermoelectric element movement module
a first gear connected to the driving motor;
a guide rail coupled to the lower frame and guiding a movement path of the thermoelectric element;
a guide block coupled to the guide rail and connected to the thermoelectric element to slide the guide rail; and
A temperature control device of a gene amplification device including a second gear coupled to the guide block and meshed with the first gear. According to claim 2,
The reaction vessel,
A well block having at least one groove formed thereon; and
A temperature control device of a gene amplification device comprising a tube containing the sample fitted into the groove. According to claim 10,
a height adjustment pillar coupled to the lower frame and extending in a first direction;
an upper plate coupled to the height-adjusting pillar, protruding in a second direction crossing the first direction, and adjustable in height in the first direction; and
The temperature control device of the gene amplification device further comprises a head coupled to the upper plate and including a protruding part formed to correspond to the groove of the well block.

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