KR101427038B1 - nucleic acid amplification apparatus - Google Patents

nucleic acid amplification apparatus Download PDF

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Publication number
KR101427038B1
KR101427038B1 KR1020130022602A KR20130022602A KR101427038B1 KR 101427038 B1 KR101427038 B1 KR 101427038B1 KR 1020130022602 A KR1020130022602 A KR 1020130022602A KR 20130022602 A KR20130022602 A KR 20130022602A KR 101427038 B1 KR101427038 B1 KR 101427038B1
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nucleic acid
block
reaction tube
acid amplification
moving table
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KR1020130022602A
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Korean (ko)
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이현영
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이현영
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
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  • Immunology (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to an apparatus for amplifying a nucleic acid, comprising: a body part (11) having a chamber (111); a fixing part (12) arranged in a center part of the chamber (111) for fixing a reaction tube module (20); a moving table (13) connected to the inside of the chamber (111); a constant high temperature block (14) connected to one side of the moving table (13); a constant low temperature block (15) connected to the other side of the moving table (13), which is arranged facing the constant high temperature block (14); and a power module (16) for moving the moving table (13) right and left.

Description

A nucleic acid amplification apparatus,

The present invention relates to a nucleic acid amplification apparatus, and more particularly, to a nucleic acid amplification apparatus in which nucleic acid amplification reagent mixture is brought into contact with constant temperature blocks alternately at different temperatures.

In general, nucleic acid amplification technology is very important technology for molecular genetic analysis, and it is very important technology for analysis of human genome research, detection of infectious diseases and genetic diseases. The PCR (Polymerase Chain Reaction) method, which is a typical technique of this nucleic acid amplification technology, is the most widely known technology, and is a technique capable of selectively amplifying a specific nucleic acid sequence in a large amount. The general PCR method amplifies a specific nucleotide sequence in a large amount by repeating the procedure of denaturation and anealing extension. Therefore, in order to repeat this procedure of denaturation and anealing extension, the nucleic acid amplification reagent should be changed to the respective set temperature. A Peltier device is most commonly used for the above-described temperature repetition, and a device for amplifying a nucleic acid by performing temperature cycling of heating and cooling in a single block is typically used. However, the nucleic acid amplification apparatus in which the block itself is repeatedly heated and cooled requires a high-precision temperature control technique and a time required for raising and lowering the temperature, thereby complicating the structure of the apparatus and increasing the reaction time.

Korean Patent Laid-Open No. 10-2012-0075918 (multi-chamber reactor for nucleic acid amplification and analysis) is disclosed in the related art.

The present invention intends to provide a nucleic acid amplification apparatus capable of nucleic acid amplification more quickly and simply by bringing two constant temperature blocks into contact with a nucleic acid amplification solution alternately.

According to an aspect of the present invention,

A body portion 11 having a chamber 111 formed therein;

A fixing part (12) formed at the center of the chamber (111) so that the reaction tube module (20) can be fixed;

A moving table (13) coupled to the inside of the chamber (111);

A high temperature thermostat block (14) coupled to one side of the moving table (13);

A low-temperature thermostat block (15) coupled to the other side of the moving table (13) and arranged to face the thermostatic thermostat block (14);

And a power module (16) capable of moving the moving table (13) to the left and right.

Also,

The fixing part 12 is a body part hole formed in the direction of the chamber 111 from the upper part of the body part 11,

The reaction tube module (20)

A plurality of reaction tube units (21) capable of containing a nucleic acid amplification substance;

A frame (22) having a plurality of fixing holes (221) capable of fixing the plurality of reaction tube units (21), respectively;

And a cover member (23) which can cover the plurality of reaction tube units (21).

Also,

A plurality of grooves 141 are formed in the high temperature thermostatic block 14,

The groove 141 is open at the upper side and the side of the low-temperature and constant-temperature block direction 15,

A heat generator 142 is coupled to the high temperature thermostat block 14.

Also,

A plurality of grooves 151 are formed in the low-temperature thermostatic block 15,

The groove 151 is open at the upper side and the side in the direction of the high temperature thermostat block 14,

A heating device 152 and a cooling device 153 are coupled to the low-temperature thermostat block 15.

The present invention provides a nucleic acid amplification device capable of nucleic acid amplification more quickly and easily by making two thermostable blocks (high temperature thermostatic block and low temperature thermostat block) alternately contact with nucleic acid amplification solution.

1 is a perspective view of a nucleic acid amplification apparatus according to an embodiment of the present invention;
2 is a perspective view illustrating a process of inserting a reaction tube module containing a nucleic acid amplification solution into a nucleic acid amplification apparatus according to an embodiment of the present invention.
3 is a cross-sectional view of a nucleic acid amplification apparatus according to an embodiment of the present invention.
4 is a perspective view of a drive module according to an embodiment of the present invention;
5 is a cross-sectional view of a drive module of a nucleic acid amplification device according to an embodiment of the present invention (with a reaction tube module in contact with a high temperature thermostat block).
FIG. 6 is a cross-sectional view of a driving module of a nucleic acid amplification apparatus according to an embodiment of the present invention (a reaction tube module is in contact with a low-temperature thermostat block).
7 is an exploded perspective view of a reaction tube module according to an embodiment of the present invention.
8 is a perspective view of a reaction tube module according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Reference will now be made in detail to the preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the like elements throughout. , Thereby not limiting the spirit and scope of the present invention.

FIG. 1 is a perspective view of a nucleic acid amplification apparatus according to an embodiment of the present invention, FIG. 2 is a perspective view illustrating a process of inserting a reaction tube module containing a nucleic acid amplification solution into a nucleic acid amplification apparatus according to an embodiment of the present invention, FIG. 3 is a cross-sectional view of a nucleic acid amplification apparatus according to an embodiment of the present invention, FIG. 4 is a perspective view of a driving module according to an embodiment of the present invention, and FIG. 5 is a schematic view of a nucleic acid amplification apparatus according to an embodiment of the present invention. 6 is a cross-sectional view of a driving module of a nucleic acid amplification apparatus according to an embodiment of the present invention (in which a reaction tube module is in contact with a low-temperature thermostat block) FIG. 7 is an exploded perspective view of a reaction tube module according to an embodiment of the present invention, and FIG. 8 is a perspective view of a reaction tube module according to an embodiment of the present invention.

The nucleic acid amplification apparatus 10 of this embodiment includes a body portion 11 having a chamber 111 formed therein; A fixed part 12 formed at the center of the chamber 111 so that the reaction tube module 20 can be fixed; a moving table 13 coupled to the inside of the chamber 111; A low temperature thermostatic block 15 disposed on the other side of the moving table 13 and facing the high temperature thermostatic block 14, a thermostatic thermostatic block 14 coupled to one side of the moving table 13, 13 to the right and left.

The body part 11 forms an outer appearance of the nucleic acid amplifying device 10, and various electronic parts and electronic elements are built in. A display device can be additionally coupled to the outside. An input module for operating the nucleic acid amplification apparatus 10 may be coupled to the body part 11. [

A chamber 111 is formed inside the body part 11. [ The reaction tube module 20 can be coupled to the inside of the chamber 111. [ At the center of the chamber 111, a fixing part 12 to which the reaction tube module 20 can be fixed is formed. The fixing part 12 may be a body part hole formed in the body part 11. [ Most of the reaction tube module 20 enters into the chamber 111 by covering the top of the reaction tube module 20 in the body cavity.

A moving table (13) is coupled to the inside of the chamber (111). The moving table 13 may be horizontally coupled to the chamber 111. The moving table 13 can reciprocate right and left by the operation of the power module 16. (See Fig. 3)

A high temperature thermostatic block 14 is coupled to one side of the upper part of the moving table 13. On the other side of the upper portion of the moving table 13, a low-temperature thermostat block 15 is coupled. The low-temperature thermostat block 15 is arranged to face the thermostatic thermostat block 14.

The high temperature thermostat block 14 is preferably maintained at about 90 degrees Celsius to about 95 degrees Celsius. A plurality of grooves 141 are formed in the high temperature thermostatic block 14 and the side surfaces of the upper portion and the low temperature thermostatic block 15 are opened. A heating device 142 is coupled to the high temperature thermostat block 14. The heating device 142 may be a Peltier device.

The low temperature thermostat block 15 is preferably maintained at about 50 degrees Celsius to about 70 degrees Celsius. A plurality of grooves 151 are formed in the low-temperature thermostat block 15, and the grooves 151 are open at the upper side and the side in the direction of the thermostatic block 14, and the thermostat 152 And a cooling device 153 may be combined. By the control of the heating device 152 and the cooling device 153, the low-temperature thermostatic block 15 can be maintained at about 50 degrees Celsius to about 70 degrees Celsius.

The upper part of the reaction tube module 20 can be fixed to the fixing part 12. The fixing part 12 may be a body part hole formed in the direction of the chamber 111 from the upper part of the body part 11. [

The reaction tube module (20) comprises: a plurality of reaction tube units (21) capable of containing nucleic acid amplification material; A frame (22) having a plurality of fixing holes (221) capable of fixing a plurality of reaction tube units (21), respectively; And a cover member 23 which can cover the plurality of reaction tube units 21. (See Fig. 7)

The power module 16 is composed of a combination of a motor and a gear, and moves the moving table 13 repeatedly to the left and right in a forward rotation of the motor. The method of moving the moving table 13 may be a combination of a linear gear coupled to the lower portion of the moving table 13 and a circular sawtooth gear coupled to the motor. The power module 16 may move the moving table 13 using a chain or a belt.

In the case of FIG. 5, the high temperature thermostat block 14 contacts the reaction tube module 20 to raise the temperature of the nucleic acid amplification material in the reaction tube module 20. 6, the moving table 13 is moved to the left side, and the low-temperature thermostat block 15 is moved to the reaction tube module 20 . Thus, as the moving table 13 moves to the left and right, the reaction tube module 20 comes into contact with the high temperature temperature block 14 or the low temperature temperature block 15. Therefore, the reaction tube module 20 is placed in a high-temperature and low-temperature environment in a timely manner.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. Modifications and additions by those skilled in the art to an equivalent range based on the embodiments will also fall within the scope of the present invention.

The nucleic acid amplification apparatus 10 includes a chamber 111,
Body portion (11) Reaction tube module (20)
The fixing part (12)
Blocks of a high temperature (14) Blocks of a low temperature (15)
Power module (16)

Claims (4)

A body portion 11 having a chamber 111 formed therein;
A fixing part (12) formed at the center of the chamber (111) so that the reaction tube module (20) can be fixed;
A moving table (13) coupled to the inside of the chamber (111);
A high temperature thermostat block (14) coupled to one side of the moving table (13);
A low-temperature thermostat block (15) coupled to the other side of the moving table (13) and arranged to face the thermostatic thermostat block (14);
And a power module (16) capable of moving the moving table (13) to the left and right, the nucleic acid amplifying device comprising:
The fixing part 12 is a body part hole formed in the direction of the chamber 111 from the upper part of the body part 11,
The reaction tube module (20)
A plurality of reaction tube units (21) capable of containing a nucleic acid amplification substance;
A frame (22) having a plurality of fixing holes (221) capable of fixing the plurality of reaction tube units (21), respectively;
And a cover member (23) for covering the plurality of reaction tube units (21).

The method according to claim 1,
A plurality of grooves 141 are formed in the high temperature thermostatic block 14,
The groove 141 is open at the upper side and the side in the direction of the low-temperature thermostatic block 15,
And a heat generator (142) is coupled to the high temperature thermostat block (14).

3. The method of claim 2,
A plurality of grooves 151 are formed in the low-temperature thermostatic block 15,
The groove 151 is open at the upper side and the side in the direction of the high temperature thermostat block 14,
Wherein a heating device (152) and a cooling device (153) are coupled to the low-temperature thermostat block (15).

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KR1020130022602A 2013-03-04 2013-03-04 nucleic acid amplification apparatus KR101427038B1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016017832A1 (en) * 2014-07-30 2016-02-04 이현영 Nucleic acid amplification device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4103964B2 (en) * 1993-10-20 2008-06-18 ストラッタジーン Thermal cycle apparatus including a temperature gradient block
KR20110118572A (en) * 2010-04-23 2011-10-31 나노바이오시스 주식회사 Device for amplify nucleic acid comprising two heating block

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4103964B2 (en) * 1993-10-20 2008-06-18 ストラッタジーン Thermal cycle apparatus including a temperature gradient block
KR20110118572A (en) * 2010-04-23 2011-10-31 나노바이오시스 주식회사 Device for amplify nucleic acid comprising two heating block

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016017832A1 (en) * 2014-07-30 2016-02-04 이현영 Nucleic acid amplification device

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