KR20220154918A - Cosmetic composition containing Cudrania extract as an active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition which has various functions beneficial to the skin, including skin whitening, wrinkle reduction, and the like, by including a Cudrania tricuspidata extract, jojoba seed oil, and a red ginseng fermented filtrate. The Cudrania tricuspidata extract is a Cudrania tricuspidata bark extract obtained by extracting the bark of Cudrania tricuspidata.

Description

Cosmetic composition containing Cudrania extract as an active ingredient {Cosmetic composition containing Cudrania extract as an active ingredient}

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition having various functions beneficial to the skin, such as skin whitening and wrinkle improvement, including Cudrania mulberry extract, jojoba seed oil and red ginseng fermented filtrate.

In general, cosmetics are used for the purpose of covering skin defects, covering skin, or emphasizing a specific part of skin.

Types of cosmetics include base makeup cosmetics, point makeup cosmetics, etc. Base makeup cosmetics include skin, lotion, foundation, etc., and point makeup cosmetics include lipstick, eyeliner, eye shadow, and the like.

Conventional cosmetics were mainly used for decorating the skin, but with the advent of modern times, functional products having functions such as skin whitening and wrinkle improvement are being released to have effects on the health or beauty of the skin.

However, in order to configure functional products, the composition is often composed of chemical substances. As a product related to this, Korean Registered Patent No. 10-2201385 (2021.01.11. Notice), "Composition for skin whitening and whitening using the same" Cosmetics for use" has been disclosed. This prior patent relates to a composition for skin whitening using sulforaphane and cosmetics for whitening using the same.

However, the conventional skin whitening composition and whitening cosmetics using the same use a sulforaphane raw material and may be irritating to the skin depending on the content thereof, and thus the need for a more safe natural product-oriented cosmetic composition is increasing.

Republic of Korea Patent Registration No. 10-2201385 (2021.01.11. notice), "Composition for skin whitening and cosmetics for whitening using the same"

In order to solve the above problems, an object of the present invention is to provide a cosmetic composition having various functions beneficial to the skin, such as skin whitening and wrinkle improvement, including Cudrania mulberry extract, jojoba seed oil and red ginseng fermented filtrate. .

In order to achieve the above object, the present invention provides a cosmetic composition containing a Cudrania quince extract as an active ingredient, characterized in that it comprises a mixture of a Cudrania quince extract, jojoba seed oil and red ginseng fermentation filtrate.

In addition, the kuji mulberry extract is characterized in that it is obtained by extracting the bark of the cudra mulberry tree extract.

In addition, the red ginseng fermentation product is characterized in that it is a lactobacillus / red ginseng fermentation filtrate obtained by fermenting red ginseng using lactobacillus and filtering it.

In addition, it is characterized in that it is formulated into a skin, lotion, serum, cream formulation, including a mixture of the kkuji mulberry extract, jojoba seed oil, and red ginseng fermentation filtrate.

The cosmetic composition containing the bark extract of Cudrania mulberry according to the present invention as an active ingredient has various functions beneficial to the skin, such as skin whitening and wrinkle improvement, including the extract of Cudrania mulberry, jojoba seed oil and fermented red ginseng filtrate.

1 is a graph showing the skin whitening effect of the composition prepared in Examples 1 to 2, Comparative Examples 1 to 9, and Preparation Example 1.
Figure 2 is a graph showing the anti-wrinkle effect of the composition prepared in Examples 1 to 2, Comparative Examples 1 to 9, and Preparation Example 1.
Figure 3 is a graph showing the anti-pollution effect of the composition prepared in Examples 1 to 2, Comparative Examples 1 to 9, and Preparation Example 1.

The detailed description of the present invention below refers to the accompanying drawings shown as examples of embodiments in which the present invention can be practiced. These embodiments are described in detail so that those skilled in the art will be able to practice the present invention. It should be understood that the various embodiments of the present invention are different, but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in one embodiment in another embodiment without departing from the spirit and scope of the invention. Additionally, it should be understood that the location or arrangement of individual components within each described embodiment may be changed without departing from the spirit and scope of the invention.

Accordingly, the detailed description set forth below is not to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all equivalents as claimed by those claims. Like reference numbers in the drawings indicate the same or similar function throughout the various aspects.

The terms used in the present invention have been selected from general terms that are currently widely used as much as possible while considering the functions in the present invention, but these may vary depending on the intention of a person skilled in the art or precedent, the emergence of new technologies, and the like. In addition, in a specific case, there is also a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, not simply the name of the term.

In the present invention, when a part "includes" a certain component, it means that it may further include other components, not excluding other components, unless otherwise stated.

Hereinafter, the cosmetic composition containing the kkujimul tree extract according to the present invention as an active ingredient will be described in detail.

The cosmetic composition containing the Cudrania sinensis extract according to the present invention as an active ingredient is characterized by comprising a mixture of Cudrania sinensis extract, jojoba seed oil, and red ginseng fermentation filtrate.

The kkujimul tree extract refers to an extract obtained from the kkujimul tree. In the present invention, the kkujimul tree extract is preferably obtained by extracting the bark of the kkujimul tree in more detail. The Cudrania tricuspidata (Carriere) Bureau ex Lavallee ) is a small tree belonging to the genus Cudrania, 3-8m high, 3-8m tall, with thorns on branches, leaves ovate, sometimes split into 3 parts, 6-10cm long, 3-6cm wide, It gradually narrows at the top and becomes dull at the end. The flowers of the kuji mulberry tree bloom in male and female plants in May-June, and in the male inflorescence, small flowers gather to form a head shape, and short and soft hairs gather.

The jojoba seed oil refers to oil obtained from the seeds of jojoba. The jojoba ( Simmondsia chinensis ) is a shrub of the jojoba family ( Simmondsiaceae ), and is cultivated to obtain oil (liquid wax ester) from seeds. The jojoba is native to the desert regions of Arizona, California and Mexico in the United States. The jojoba seed oil is distributed on the market under the INCI name SIMMONDSIA CHINENSIS (JOJOBA) SEED OIL, and it may be possible to directly purchase and use jojoba seed oil on the market.

The red ginseng fermentation filtrate refers to a filtrate obtained by fermenting and filtering red ginseng. More specifically, the red ginseng fermentation product is preferably a lactobacillus/red ginseng fermentation filtrate obtained by fermenting red ginseng using a Lactobacillus strain and filtering it. The red ginseng is a light yellowish brown or light reddish brown color obtained by carefully selecting good quality 6-year-old fresh ginseng produced in suitable areas for ginseng (eng CA Meyer) cultivation, steaming for a long time in an unpeeled state and drying it, and the size and shape of ginseng, etc. It is classified into 1 to 5 grades after washing, steaming, and drying to turn into red ginseng. Red ginseng is distributed on the market and is easy to obtain, and Lactobacillus strains can also be purchased and used on the market. Alternatively, the lactobacillus / red ginseng fermentation filtrate is distributed in the market under the INCI name LACTOBACILLUS / GINSENG ROOT FERMENT FILTRATE, and the lactobacillus / red ginseng fermentation filtrate may be directly purchased and used.

The cosmetic composition containing the Cudrania mulberry extract according to the present invention as an active ingredient includes a mixture of Cudrania mulberry extract, jojoba seed oil, and red ginseng fermentation filtrate, which contains the Cudrania mulberry extract, jojoba seed oil, and red ginseng fermentation filtrate at the same time. it means.

The mixture of the Cudrania mulberry extract, jojoba seed oil and red ginseng fermentation filtrate is preferably a mixture of the Cudrania mulberry extract, jojoba seed oil and red ginseng fermentation filtrate in a weight ratio of 0.01 to 50.0: 0.01 to 50.0: 0.01 to 50.0, respectively.

More preferably, the mixture of the Cudrania tree extract, jojoba seed oil and red ginseng fermentation filtrate is most preferably a mixture of the Cudrania tree extract, jojoba seed oil and red ginseng fermentation filtrate in a weight ratio of 34.0: 33.0: 33.0, respectively.

In specific implementation, the cosmetic composition containing the kkujimul tree extract according to the present invention as an active ingredient may be a cream formulation. Components that may be additionally included when the cosmetic composition containing the kkujimul tree extract according to the present invention as an active ingredient in skin, lotion, serum, and cream formulations are shown in Table 1 below.

Ingredients INCI name Purified water WATER glycerin GLYCERIN Caprylic/Capric Triglyceride CAPRYLIC/CAPRIC TRIGLYCERIDE Butylene Glycol Dicaprylate/Dicaprate BUTYLENE GLYCOL DICAPRYLATE/DICAPRATE betaine BETAINE Cetearyl Alcohol CETEARYL ALCOHOL 1,2-Hexanediol 1,2-HEXANEDIOL jojoba seed oil SIMMONDSIA CHINENSIS (JOJOBA) SEED OIL shea butter BUTYROSPERMUM PARKII(SHEA) BUTTER Cudrania Bark Extract CUDRANIA TRICUSPIDATA BARK EXTRACT Butylene Glycol BUTYLENE GLYCOL trehalose TREHALOSE Glyceryl Stearate GLYCERYL STEARATE beeswax BEESWAX Cetearyl Olivate CETEARYL OLIVATE carbomer CARBOMER tromethamine TROMETHAMINE Sorbitan Olivate SORBITAN OLIVATE Cetearyl Glucoside CETEARYL GLUCOSIDE Sodium Acrylate/Sodium Acryloyl Dimethyl Taurate Copolymer SODIUM ACRYLATE/SODIUM ACRYLOYLDIMETHYL TAURATE COPOLYMER polyisobutene POLYISOBUTENE Ethylhexylglycerin ETHYLHEXYLGLYCERIN beta-glucan BETA-GLUCAN propanediol PROPANEDIOL sodium hyaluronate SODIUM HYALURONATE Spices FRAGRANCE Disodium EDT DISODIUM EDTA Lactobacillus/red ginseng fermentation filtrate LACTOBACILLUS/GINSENG ROOT FERMENT FILTRATE

That is, the cosmetic composition containing the Cudrania sinensis extract according to the present invention as an active ingredient is purified water, glycerin, caprylic / capric triglyceride, butylene glycol dicaprylate / dicaprate, betaine, cetea Lil Alcohol, 1,2-Hexanediol, Jojoba Seed Oil, Shea Butter, Cudrania Bark Extract, Butylene Glycol, Trehalose, Glyceryl Stearate, Beeswax, Cetearyl Olivate, Carbomer, Tromethamine, Sorbitan Olivate, Cetearyl Glucoside, Sodium Acrylate/Sodium Acryloyldimethyltaurate Copolymer, Polyisobutene, Ethylhexylglycerin, Beta-Glucan, Propanediol, Sodium Hyaluronate, It may contain a mixture of fragrance, disodium EDTA, and lactobacillus/red ginseng fermented filtrate at the same time. In this case, the fragrance may be menthol.

Hereinafter, the effect of the cosmetic composition containing the Cudrania mulberry extract according to the present invention as an active ingredient will be examined in detail through the following Examples, Comparative Examples and Experimental Examples.

Example One. mulberry tree extract, jojoba seed oil and red ginseng fermentation filtrate Preparation of compositions comprising mixtures

According to the following manufacturing process, a composition containing a mixture of kkuji mulberry extract, jojoba seed oil and red ginseng fermentation filtrate was prepared.

34.0% by weight of Cudrania extract, 33.0% by weight of jojoba seed oil, and 33.0% by weight of red ginseng fermented filtrate were put in a circular beaker and stirred for 30 minutes at 120 rpm using an Aji mixer to prepare a composition.

Example 2. Cudrania Bark extract, jojoba seed oil and Lactobacillus / red ginseng fermentation filtrate Compositions containing mixtures

According to the following manufacturing process, a composition containing a mixture of kuji mulberry bark extract, jojoba seed oil, and lactobacillus/red ginseng fermentation filtrate was prepared.

34.0% by weight of Cudrania bark extract, 33.0% by weight of jojoba seed oil, and 33.0% by weight of lactobacillus/red ginseng fermented filtrate were placed in a circular beaker and stirred for 30 minutes at 120 rpm using an aji mixer to prepare a composition.

comparative example One. mulberry tree A composition containing only the extract

According to the following manufacturing process, a composition containing only the kkuji mulberry extract alone was prepared.

A composition was prepared by putting 34.0% by weight of Cudrania mulberry extract into a beaker, adding purified water as the remaining amount, and then stirring for 30 minutes at 120 rpm using an Aji mixer.

comparative example 2. Cudrania Bark A composition containing only the extract

According to the following manufacturing process, a composition containing only the kkujimul bark extract alone was prepared.

A composition was prepared by putting 34.0% by weight of Cudrania bark extract in a beaker, adding purified water as the remaining amount, and then stirring for 30 minutes at 120 rpm using an aji mixer.

comparative example 3. Jojoba seed oil only Compositions containing alone

According to the following manufacturing process, a composition containing only jojoba seed oil was prepared.

After putting 33.0% by weight of jojoba seed oil in a beaker and adding the remaining amount of purified water, the mixture was stirred at 120 rpm for 30 minutes using an azimixer to prepare a composition.

comparative example 4. Red ginseng fermentation filtrate only Compositions containing alone

According to the following manufacturing process, a composition containing only the red ginseng fermentation filtrate was prepared.

After putting 33.0% by weight of the red ginseng fermented filtrate in a beaker and adding the remaining amount of purified water, the mixture was stirred at 120 rpm for 30 minutes using an Azimixer to prepare a composition.

comparative example 5. Lactobacillus / Red ginseng fermentation filtrate only Compositions containing alone

According to the following manufacturing process, a composition containing only the lactobacillus/red ginseng fermented filtrate was prepared.

33.0% by weight of the lactobacillus/red ginseng fermented filtrate was put in a beaker, the remaining amount of purified water was added, and then the mixture was stirred at 120 rpm for 30 minutes using an azimixer to prepare a composition.

manufacturing example One. Cudrania Bark extract, jojoba seed oil and Lactobacillus / red ginseng fermentation filtrate containing a mixture creamy Produce

Purified water, glycerin, caprylic/capric triglyceride, butylene glycol dicaprylate/dicaprate, betaine, cetearyl alcohol, 1,2-hexanediol, jojoba seed oil, shea butter, Cudrania Bark Extract, Butylene Glycol, Trehalose, Glyceryl Stearate, Beeswax, Cetearyl Olivate, Carbomer, Tromethamine, Sorbitan Olivate, Cetearyl Glucoside, Sodium Acrylate/Sodium Acryloyldimethyltaurate copolymer, polyisobutene, ethylhexylglycerin, beta-glucan, propanediol, sodium hyaluronate, fragrance (menthol), disodium EDTA and lactobacillus/red ginseng fermentation filtrate Each was mixed in an equal weight ratio, put into a beaker, and stirred at 120 rpm for 30 minutes using an Azimixer to prepare a cream formulation.

Experimental example One. skin whitening Check the effect

The skin whitening effect of the compositions prepared in Examples 1 to 2, Comparative Examples 1 to 5, and Preparation Example 1 was confirmed.

After adding 5 to 6 ml of DMEM medium (89% glucose 4.5g/L, 10% serum, 1% antibiotics) to a 25cm2 T-flask, cryopreserved mouse-derived B-16 melanoma (ATCC CRL 6323) cell line was frozen. Inoculated and cultured for 24 hours under 37°C temperature and 5% CO ₂ conditions, then treated with 0.05% trypsin containing 0.02% EDTA to separate the cells, inoculated into a 25cm2 T-flask and cultured for 48 hours. . As a result, the number of cells obtained was 5.76 × 10 ⁶ cells per flask. Here, 100 μg / ml of Examples 1 to 2, Comparative Examples 1 to 5, and the composition prepared in Preparation Example 1 was diluted in DMEM medium, treated with cultured melanoma cells, and cultured at 37 ° C. for 5 days. After culturing, all the medium was removed, and the cells were separated by treatment with 1 ml of saline-phosphate buffer (PBS) containing 0.02% EDTA and 0.05% trypsin, and only the cells were collected by centrifugation for 5 minutes. The collected cells were treated with 5% trichloroacetate (TCA), stirred, centrifuged, and precipitated melanin was washed with a saline-phosphate buffer solution. Subsequently, after dissolving the precipitated melanin by treating it with 1N NaOH, the absorbance was measured at 475 nm, the concentration of melanin was determined from the standard concentration curve of synthetic melanin (Sigma), and the melanin production inhibition rate was shown in Table 2 below.

Melanin production inhibition rate (%) Example 1 63.3 Example 2 71.2 Comparative Example 1 28.2 Comparative Example 2 31.4 Comparative Example 3 25.6 Comparative Example 4 27.3 Comparative Example 5 28.4 Preparation Example 1 74.2

As a result, it was confirmed that the skin whitening effect of Examples 1 and 2, in which all components were mixed, had a significantly higher value than Comparative Examples 1 to 5, in which each component was treated alone, and unexpected It was confirmed that a synergistic effect appeared. On the other hand, it was confirmed that Example 2 had a higher whitening effect than Example 1.

Experimental example 2. Check wrinkle improvement effect

The anti-wrinkle effect of the compositions prepared in Examples 1 to 2, Comparative Examples 1 to 5, and Preparation Example 1 was confirmed.

Elastase is involved in the decomposition of elastin, a matrix protein that maintains skin elasticity in the dermis, and is a non-specific hydrolase capable of degrading elastin. In addition, it is one of the leukocyte aggregation enzymes that decompose elastin in the body, and its activity is increased in abnormal tissues, which is a direct cause of tissue destruction, and causes wrinkles and loss of elasticity of the skin. Therefore, elastase inhibitors have the effect of improving skin wrinkles.

Porcine pancreas elastase inhibitory activity was measured using N-succinyl-(L-Ala)3-p-nitroanilide as a substrate at 37°C for 20 minutes by p-nitroanilide The amount of production was measured at 405 nm. That is, the compositions prepared in Examples 1 to 2, Comparative Examples 1 to 5, and Preparation Example 1 were prepared to have a concentration of 100 μg/ml, and 0.5 ml each was taken in a test tube and placed in a 50 mM Tris-HCl buffer (pH 8.6). After adding 0.5 ml of the dissolved elastase (2.5 U/ml) solution, N-succinyl-(LAla)3-p-nitroanilide (0.5 mg/ml) dissolved in 50 mM Tris-HCl buffer (pH 8.6) was used as a substrate. ㎖) was added and reacted for 20 minutes to measure. As a control group, ursolic acid was used, and the results are shown in Table 3 below as a percentage compared to ursolic acid as a control group.

Elastase inhibition (%) Control (ursolic acid) 100% Example 1 101.3% Example 2 103.2% Comparative Example 1 60.8% Comparative Example 2 65.2% Comparative Example 3 55.6% Comparative Example 4 57.4% Comparative Example 5 59.1% Preparation Example 1 105.8%

As a result, it was confirmed that the elastase inhibitory effect of Examples 1 and 2, in which all components were mixed, had a significantly higher value than Comparative Examples 1 to 5, in which each component was treated alone. It was confirmed that an unexpected synergistic effect appeared. On the other hand, it was confirmed that Example 2 can have a higher anti-wrinkle effect than Example 1.

Experimental example 3. anti-pollution Check the effect

The anti-pollution effect of the compositions prepared in Examples 1 to 2, Comparative Examples 1 to 5, and Preparation Example 1 was confirmed.

In general, the degree of cell damage by pollutants is measured using skin keratinocytes that come into direct contact with pollutants, and the compositions prepared in Examples 1 to 2, Comparative Examples 1 to 5, and Preparation Example 1 are treated, , The effect of inhibiting skin cell damage was confirmed. The experiment was conducted according to a known method.

Human keratinocyte HaCaT was dispensed at a concentration of 1×10 ⁴ cells/ml in 200 µl each in a 96-well plate, and cultured in a CO ₂ incubator at 37°C for 24 hours. At this time, IMDM medium containing 10% of bovine serum and 1% of antibiotics was used as the medium. After culturing, the medium was replaced with IMDM medium excluding bovine serum and antibiotics, and then treated with benzopyrene (2.5 μg/mL), ammonium nitrate (500 ppm), and formaldehyde (1.5 μg/mL), and then Examples 1 to 2 and Comparative Example 1 to 5, and the composition prepared in Preparation Example 1 was diluted 1000 times and further cultured for 21 hours. Thereafter, 5 mg/ml MTT solution was added and incubated for 3 hours, then the medium was removed, 150 μl of dimethyl sulfoxide was added to each well, shaken for 10 minutes, and absorbance was measured at 570 nm. The cell growth rates of the compositions prepared in Examples 1 to 2, Comparative Examples 1 to 5, and Preparation Example 1 were compared with the cell viability of the experimental group not treated with contaminants as a control and the cell growth rate of the control group as 100%. In the control group, only purified water was added instead of the experimental group.

Cell proliferation rate (%) for benzopyrene (2.5 μg/mL) Cell proliferation rate (%) for ammonium nitrate (500 ppm) Cell proliferation rate (%) for formaldehyde (1.5 μg/mL) Control group (no contaminant treatment group) 100 100 100 Control group (experimental group, untreated group) 41 43 38 Example 1 81 82 79 Example 2 84 85 82 Comparative Example 1 48 50 48 Comparative Example 2 49 51 50 Comparative Example 3 42 43 41 Comparative Example 4 43 44 40 Comparative Example 5 45 45 42 Preparation Example 1 85 86 84

As a result, it was confirmed that the anti-pollution effect of Examples 1 and 2, in which all components were mixed, had a significantly higher value than Comparative Examples 1 to 5, in which each component was treated alone. It was confirmed that a synergistic effect was not found. On the other hand, it was confirmed that Example 2 had a higher anti-pollution effect than Example 1.

Although the present invention has been described with the accompanying drawings, this is only one example of various embodiments including the gist of the present invention, and is intended to be easily practiced by those skilled in the art. As an object, it is clear that the present invention is not limited to the embodiments described above. Therefore, the protection scope of the present invention should be construed by the following claims, and all technical ideas within the equivalent range by change, substitution, substitution, etc. will be included In addition, it is clear that some configurations in the drawings are provided exaggerated or reduced than the actual ones to more clearly explain the configuration.

Claims (5)

A cosmetic composition containing a Cudrania quince extract as an active ingredient, characterized in that it comprises a mixture of Cudrania quince extract, jojoba seed oil and red ginseng fermentation filtrate. According to claim 1,
The cosmetic composition containing the kuji mulberry extract as an active ingredient, characterized in that the kuji mulberry extract is an extract of the cudra mulberry bark obtained by extracting the bark of the cudra mulberry tree. According to claim 1,
The red ginseng fermentation product is a cosmetic composition containing as an active ingredient, a lactobacillus / red ginseng fermentation filtrate obtained by fermenting red ginseng using lactobacillus and filtering it. According to claim 1,
The mixture is a cosmetic composition containing a kuji mulberry extract as an active ingredient, characterized in that the kuji mulberry extract, jojoba seed oil, and red ginseng fermentation filtrate are mixed in a weight ratio of 0.01 to 50.0: 0.01 to 50.0: 0.01 to 50.0, respectively. According to claim 1,
A cosmetic composition containing the Cudrania quince extract as an active ingredient, characterized in that it is formulated into a cream formulation, including a mixture of the Cudrania quince extract, jojoba seed oil and red ginseng fermentation filtrate.

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