KR20220109819A - A fermented plant oil for anti-pollution, and a cosmetic composition comprising the same - Google Patents

Abstract

The present invention provides a vegetable oil for preventing or removing skin fine dust fermented by a Pseudozyma sp. strain. The fermented vegetable oil has excellent emulsification stability, thereby being suitable as a cosmetic raw material, and in particular, having the excellent effect of preventing and removing fine dust.

Description

Anti-pollution fermented vegetable oil, and a cosmetic composition comprising the same

The present invention relates to a fermented vegetable oil having the effect of adsorbing and removing heavy metals or fine dust remaining in skin pores and cosmetics containing the same.

External pollutants such as rapid industrialization, automobile exhaust gas, heavy metal powder and fine dust caused by air masses from China contaminate people's skin, and are considered a major cause of skin aging and skin troubles.

Heavy metals refer to heavy metal elements with a specific gravity of 4 or more, such as lead, mercury, cadmium, zinc, and nickel.

Heavy metals cause a chemical reaction with the atmosphere in the skin and additionally create harmful substances such as nitrogen oxides (NO), which can cause skin irritation, atopy, and skin troubles.

On the other hand, fine dust is a very small substance that is invisible to our eyes and refers to particulate matter with a diameter of 10 μm or less that floats or scatters in the atmosphere for a long time.

Specifically, it is an air pollutant that comes out when fossil fuels such as coal and petroleum are burned or exhaust gases such as manufacturing and automobile soot, and is adsorbed into the lungs through the bronchi and causes various lung diseases.

Fine dust affects not only inflammatory skin diseases such as psoriasis, atopic dermatitis, and acne, but also skin aging, hair loss, and skin cancer.

Since fine dust is up to 20 times smaller than the size of pores, it can easily penetrate into the skin, and continuous attacks of fine dust can cause serious diseases by losing the protective function of the skin barrier.

In addition, it is known that the increase of inflammatory cytokines and the increase of free radicals in the body caused by fine dust increase the inflammatory response, wrinkle formation, and pigmentation in skin cells, and seriously affect the development of skin cancer.

Fine dust increases premature aging, lack of moisture, pigmentation, fine lines and wrinkles in the short term, and in the long term, irreversible skin damage causes the skin to lose its ability to protect the body from environmental factors, resulting in serious problems such as skin cancer. can cause illness.

There is a method to remove heavy metals from the body by injecting complex compound-forming substances such as EDTA (ethylene diamine tetraacetic acid) and BAL (British anti-lewisite) to bind them with the heavy metals accumulated in the body, thereby discharging the heavy metals out of the body. In addition, there is a method of preventing absorption of heavy metals into the body and facilitating their discharge by taking vitamin B1, vitamin C, and vitamin E.

However, there is no effective way to remove the heavy metals accumulated in the skin pores, and although fine dust can be removed to some extent with cleansing, the finer it is, the stronger the adsorption power, so it penetrates deeply into the pores and is completely removed with general cleansing. it is impossible

On the other hand, in the cosmetic industry, a number of products using natural products are being developed to reduce skin irritation caused by various chemicals.

Natural ingredients have fewer side effects on the skin, and as consumers' response to cosmetics using natural ingredients increases, the value of development as a cosmetic raw material is increasing.

Therefore, there is a demand for the development of a cosmetic composition that improves skin stability, including natural raw materials, and has excellent adsorption power of heavy metals or fine dust.

The present invention is to solve the problems of the prior art described above, and an object of the present invention is to provide an eco-friendly cosmetic raw material having excellent biosafety, anti-adsorption prevention and removal ability of fine dust.

According to one aspect of the present invention, there is provided a vegetable oil for preventing or removing skin fine dust adsorption fermented by the Pseudozima sp. strain.

In one embodiment, the strain may be Pseudojima genus SY16 (KCTC 8950P).

In one embodiment, the vegetable oil may be one or more selected from the group consisting of sunflower seed oil, olive oil and apricot seed oil.

In one embodiment, the vegetable oil may be fermented by adding self-inflicted herbs, olive leaves and hemp seeds.

In one embodiment, the vegetable oil may further include antioxidant, and anti-inflammatory use.

In one embodiment, the vegetable oil may inhibit TRPV1 or XRE activated by fine dust.

According to another aspect of the present invention, there is provided a cosmetic composition comprising the vegetable oil as an active ingredient.

In one embodiment, the cosmetic composition comprises a softening lotion, a nutrient lotion, a moisture cream, a nutrient cream, a massage cream, a nutrient lotion, an essence, an ampoule, a gel, an eye cream, an oil, a foundation, a cleansing cream, a cleansing foam, a cleansing water, It may be formulated with one or more selected from the group consisting of a mask pack, a spray, and a powder.

According to the present invention, the fermented vegetable oil has excellent emulsion stability, so it is suitable as a cosmetic raw material, and in particular, the effect of preventing and removing fine dust is remarkably excellent.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a result of evaluating the XRE gene expression inhibition activity according to the vegetable oil treatment according to an embodiment of the present invention. (A) is the result of treating fermented sunflower seed oil, and (B) is the result of treating unfermented sunflower seed oil.

The terms used in this specification have been selected as currently widely used general terms as possible while considering the functions in the present invention, which may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like.

In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Numerical ranges are inclusive of the values defined in that range. Every maximum numerical limitation given throughout this specification includes all lower numerical limitations as if the lower numerical limitation were expressly written. Every minimum numerical limitation given throughout this specification includes all higher numerical limitations as if the higher numerical limitation were expressly written. All numerical limitations given throughout this specification will include all numerical ranges that are better within the broader numerical limits, as if the narrower numerical limitations were expressly written.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited by the following examples.

According to one aspect of the present invention, there is provided a vegetable oil for preventing or removing skin fine dust adsorption fermented by the Pseudozima sp. strain.

The present inventors confirmed the excellent skin improvement effect of the vegetable oil fermented by the Pseudozima sp. strain, and found that the fermented vegetable oil can effectively protect the skin from fine dust.

The “fermentation” refers to a process in which microorganisms decompose organic matter using their own enzymes. Raw materials that have undergone the fermentation process through microorganisms can have at least 2 times to up to tens of times the effect of those before fermentation.

The fermented metabolites contain various amino acids, organic acids, and antioxidants that are good for the skin, and can promote skin metabolism, and the fermentation process can improve skin absorption by reducing particles and decomposing toxins.

The fermented vegetable oil may refer to fermenting a vegetable raw material and extracting a fat-soluble component and adding it to the oil, or may refer to aging by adding a vegetable raw material to the oil.

In addition, the fermented vegetable oil may refer to an oil obtained by fermenting fish or plant materials to extract a fat-soluble component or an oil extracted after immersing, germination or steaming of oil seeds.

The fermented vegetable oil of the present invention can be obtained by culturing the Pseudozima sp. strain in a culture medium under aerobic conditions, then performing secondary culture by adding vegetable oil to the culture medium, and recovering the vegetable oil. .

The culture medium has not yet added vegetable oil, and various types of culture materials can be blended so that the fermentation of vegetable oil occurs efficiently, for example, glucose, yeast extract, malt extract, peptone, and mashed soybean meal. , (NH ₄ ) ₂ SO ₄ , KH ₂ PO ₄ , MgSO ₄ , CaCl ₂ , and may include one or more from the group consisting of NaCl.

As the fermented vegetable oil is fermented by microorganisms, the free fatty acid content may be increased than before fermentation, for example, the free fatty acid content may be increased 5.0 to 140.0 times compared to before fermentation.

The Pseudojima genus ( Pseudozima sp. ) The strain may be a Pseudojima genus SY16 (KCTC 8950P) strain.

The Pseudojima genus SY16 (KCTC 8950P) strain is a microorganism capable of producing an emulsified material, proliferating in a cylindrical shape and in a polar germination type, and can form endospores distinct from hyphae and ascomytospores.

The vegetable oils include sunflower seed oil, olive oil and apricot seed oil, palm oil, argan oil, green tea seed oil, grape seed oil, meadowfoam seed oil, soybean oil, grape seed oil, sesame oil, perilla oil, flax oil, safflower seed oil , may be selected from the group consisting of corn oil, rapeseed oil, and canola oil, but preferably at least one selected from the group consisting of sunflower seed oil, olive oil and apricot seed oil.

The vegetable oil may be fermented by adding one or more selected from the group consisting of self-made herbs, olive leaves and hemp seeds.

The self-growing ( Radix Lithospermi ) is a perennial plant of the Lithospermum erythrorhizon family that lives in the meadows of mountains and fields of Korea, China, and Japan. have leaves

The self-inflicted hair has a lot of fine hairs pointing upwards as a whole, and the root is thick and purple, so it is called self-inflicted, and in oriental medicine, the root is used as a medicine.

The self-inflicted herb is effective for hematemesis, nosebleeds, urine, bleeding, and measles, and is externally used as an antiseptic for burns, frostbite, eczema, rash, and skin ulcers. The root is used for antibacterial and anti-inflammatory action, and in folklore, it is also called as an anti-inflammatory drug.

The self-inflicted substances include various active ingredients such as a mixture of deoxyshikonin, β β dimethylacrylshikonin, isobutylshikonin, shikonin, β cytosterol and caffeic acid ester. It can be, and the vegetable oil that has undergone the fermentation process by adding the self-inflicts can exhibit a more excellent anti-pollution effect.

The "olive (olive, Olea europaea Linnaeus )" is a plant of the ash family, and leaves and fruits are used for food, which is based on food standards and standards. The olive is native to the Mediterranean region and grows mainly in southern Europe, where the leaves are small and hard and relatively dry, so it is dry at high temperatures such as Spain and Italy. Oleuropein, which is abundantly contained in the olive leaf, is known to be effective in improving vascular health, anti-aging, anti-inflammatory, and preventing constipation, and hydroxytyrosol is known to be effective in inflammation.

The "Hemp seed" is a seed of hemp called hemp, hemp, and tetrahydrocannabinol, which is known as an anesthetic or hallucinogenic component, compared to cannabis obtained from the mouth and flowers of hemp. Due to its low content, it is distributed for industrial use.

The hemp seeds contain high-quality protein, essential fatty acids, vitamins, minerals, dietary fiber, and the like, so it is known to help prevent cardiovascular disease and various cancers, and it is known as a good food for diet by giving a feeling of satiety. .

The vegetable oil may further include antioxidant, and anti-inflammatory uses.

The “antioxidant” refers to the action of inhibiting oxidation, and the human body is in a balance between the oxidation promoting substance and the oxidation inhibitory substance. , oxidative stress is induced in the living body, which can cause cell damage and pathological diseases.

Reactive oxygen species (ROS), which is a direct cause of oxidative stress, is chemically unstable and highly reactive, so it can easily react with various biomaterials such as DNA, proteins, lipids and carbohydrates, It attacks and causes irreversible damage to cells and tissues or causes mutations, cytotoxicity and cancer, and is also a direct cause of aging.

By removing or reducing the reactive oxygen species, it is possible to obtain an antioxidant effect to prevent aging and maintain health.

The “anti-inflamatory” refers to an inflammatory improvement effect. The “inflammation” is a mechanism to repair and regenerate the damaged area when an invasion that causes any organic changes such as physical action, chemical substances, or bacterial infection is applied to a living body or tissue, and when stimulation is applied locally, histamine, serotonin , bradykinin, prostaglandin, HETE (hydroxyeicosatetraenoic acid), and vasoactive substances such as leukotriene are released, which can increase vascular permeability and cause inflammation.

The vegetable oil may inhibit TRPV1 or XRE activated by fine dust.

The TRPV1 (Transient Receptor Potential Vanilloid 1) protein is one of the calcium permeable non-selective cation channel family, and the activity of TRPV1 induces Ca ²⁺ influx and can be inhibited by specific antagonists such as capsazepine. have.

The TRPV1 may be activated by external stimuli such as capsaicin, bradykinin, PGE ₂ , or ATP.

In addition, the XRE (Xenobiotic Response Element) is a xenobiotic metabolic factor activated through a receptor called Aryl Hydrocarbon Receptor (AHR) of keratinocytes of the skin, and the skin regulates defense factors for protecting the skin from xenobiotics.

However, when the air pollution level is serious, the XRE is rather activated excessively in the process of protecting the skin against air pollutants, which may cause damage to the skin through an inflammatory reaction, moisture loss, and the like.

The vegetable oil can effectively protect the skin from external stimuli such as fine dust, and can effectively inhibit TRPV1 and XRE, whose expression levels are increased by external stimuli.

According to another aspect of the present invention, there is provided a cosmetic composition comprising the vegetable oil as an active ingredient.

Since the cosmetic composition contains the vegetable oil, it is possible to effectively remove fine dust adsorbed to the skin, and effectively protect the skin from external stimuli such as fine dust.

The cosmetic composition includes a softening lotion, a nourishing lotion, a moisture cream, a nourishing cream, a massage cream, a nourishing lotion, an essence, an ampoule, a gel, an eye cream, an oil, a foundation, a cleansing cream, a cleansing foam, a cleansing water, a mask pack, a spray and a powder It may be formulated with one or more selected from the group consisting of.

The cosmetic composition may be formulated by a conventional method. In the formulation of the cosmetic composition, for example, reference may be made to the content disclosed in the International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association, Inc., Washington, 1995).

Specifically, the cosmetic composition can be prepared in general emulsified formulations and solubilized formulations. For example, lotion, such as a softening lotion or a nourishing lotion; emulsions such as facial lotions and body lotions; creams such as nourishing creams, moisturizing creams, and eye creams; essence; makeup ointment; spray; gel; pack; sunscreen; makeup base; foundations such as liquid type, solid type or spray type; powder; makeup removers such as cleansing creams, cleansing lotions, and cleansing oils; Or it may be formulated as a cleaning agent such as a cleansing foam, soap, body wash, but is not limited thereto.

In the cosmetic composition, in addition to the essential components in each formulation, other components may be appropriately blended within the range that does not impair the purpose according to the present invention, depending on the type of formulation or purpose of use.

The cosmetic composition may include a conventionally acceptable carrier, for example, oil, water, surfactant, humectant, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc. may be appropriately mixed, but is limited thereto not.

The acceptable carrier may vary depending on the formulation. For example, when formulated into an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or their Mixtures may be used.

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or mixtures thereof may be used as a carrier component, and in the case of spray, chlorofluorohydro It may further contain a propellant such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, solubilizer, or emulsifier may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan may be used.

When the cosmetic composition is formulated as a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the cosmetic composition is formulated as a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionate, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars, etc. can be used

The cosmetic composition is a fatty substance, organic solvent, solubilizer, thickening agent, gelling agent, softening agent, antioxidant, suspending agent, stabilizing agent, foaming agent, and fragrance commonly used in the industry depending on the quality or function of the final product. , surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, commonly used in cosmetics It may additionally contain adjuvants commonly used in the field of cosmetology or dermatology, such as any other ingredients.

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

Through the following examples, the present invention will be described in more detail, but it is obvious that the present invention is not limited by the following examples.

Preparation Example 1: Preparation of fermented vegetable oil

Culture medium (glucose 10 g/L, yeast extract 3 g/L, malt extract 3 g/L, peptone 3 g/L, soya bean meal 3 g/L, (NH ₄ ) ₂ SO ₄ 2 g/L, KH ₂ PO ₄ 1 g/L, MgSO ₄ 0.5 g/L, CaCl ₂ 0.1 g/L, NaCl 0.1 g/L) Inoculate 1 L of Pseudojima SY16 (KCTC 8950P) strain and culture at 25° C., 300 rpm, aerobic conditions did.

Sunflower seed oil, olive oil or apricot seed oil 100L was added to the culture medium, and secondary culture was performed at 25° C., 500 rpm, and aerobic conditions.

After the secondary culture, 0.5L of the culture solution was taken for each time period, and only the oil portion was recovered.

Preparation Example 2: Preparation of fermented vegetable oil

A fermented vegetable oil was prepared in the same manner as in Preparation Example 1, but the self-inflicted pulverized product prepared by drying and crushing the culture medium was added.

Preparation Example 3: Preparation of fermented vegetable oil

A fermented vegetable oil was prepared in the same manner as in Preparation Example 1, but the mixed pulverized product of self-inflicted herb, olive leaf, and hemp seeds prepared by drying and crushing the culture medium was added.

Comparative Preparation Example 1: Preparation of vegetable oil

A separate fermentation process for sunflower seed oil, olive oil, or apricot seed oil was not performed, and the oil was used as a sample of Comparative Example.

Comparative Preparation Example 2: Preparation of fermented vegetable oil

A fermented vegetable oil was prepared in the same manner as in Preparation Example 1, but the fermentation process was performed using lactobacillus bifidus as a fermenting microorganism.

Examples and Comparative Examples

Examples (Table 1) and Comparative Examples (Table 2) were set by mixing the samples as follows to verify the skin improvement effect of the obtained sample.

[Content (parts by weight)] division Example One 2 3 4 5 6 7 8 9 10 11 12 sunflower seed oil
(Production Example 1) 300 - - 100 - - - - - - - - Olive oil (Preparation Example 1) - 300 - 100 - - - - - - - - Apricot Seed Oil (Preparation Example 1) - - 300 100 - - - - - - - - Sunflower seed oil (Preparation Example 2) - - - - 300 - - 100 - - - - Olive oil (Preparation Example 2) - - - - - 300 - 100 - - - - Apricot Seed Oil
(Production Example 2) - - - - - - 300 100 - - - - sunflower seed oil
(Production Example 3) - - - - - - - - 300 - - 100 Olive oil (Preparation Example 3) - - - - - - - - - 300 - 100 Apricot Seed Oil (Preparation Example 3) - - - - - - - - - - 300 100

[Content (parts by weight)] division comparative example One 2 3 4 5 6 7 8 sunflower seed oil
(Production Example 1) 300 - - 100 - - - - Olive oil (Preparation Example 1) - 300 - 100 - - - - Apricot Seed Oil (Preparation Example 1) - - 300 100 - - - - Sunflower seed oil (Preparation Example 2) - - - - 300 - - 100 Olive oil (Preparation Example 2) - - - - - 300 - 100 Apricot Seed Oil (Preparation Example 2) - - - - - - 300 100

Experimental Example 1: Skin safety test

In order to verify the skin safety of the samples of Examples and Comparative Examples, MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT).

After processing the samples of Examples and Comparative Examples, cell viability was measured.

Cytotoxicity was performed by modifying Mosmann's method for measuring cell viability using MTT {3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide} reagent.

Fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) were each dispensed in a 96-well plate at a concentration of 1×10 ⁴ cells/well and cultured at 37° C., 5% CO ₂ for 48 hours. did.

After removing the culture medium and culturing the sample in the treated medium for 48 hours, the medium was removed and washed repeatedly with PBS (Phosphate buffered saline).

MTT was dissolved in PBS at 5 mg/mL, 50L was added, and incubated at 37°C, 5% CO ₂ for 24 hours. 100 L of DMSO (dimethyl sulfoxide) was added per well, and the absorbance was measured at 540 nm after stirring for 10 minutes.

As a result of the measurement, the vegetable oils of Examples and Comparative Examples showed little cytotoxicity.

The above results suggest that the sample is not only harmless to the human body, but also has excellent human safety.

Experimental Example 2: Polycyclic aromatic hydrocarbon removal effect test

The adsorption effect of polycyclic aromatic hydrocarbons (PAH), a harmful air substance contained in large amounts in fine dust, was tested.

After preparing a pig skin (1 cm x 1 cm) specimen from which the hair was removed, it was washed with PBS for 10 minutes.

The washed pig skin was immersed in a solution (pH 11) containing 600 ppm of naphtalene, acenaphthylene, acenaphthene, fluorene, and benzopyrene for 2 hours at room temperature. dried.

Samples of Examples and Comparative Examples were treated with specimens and reacted for 3 hours, and PBS was applied to the control group and reacted for 3 hours.

After washing with PBS, the specimen was cut to a constant weight and pulverized with a homogenizer.

After drying the pulverized tissue with anhydrous sodium sulfate (Na ₂ SO ₄ ), polycyclic aromatic hydrocarbons were extracted from the tissue through Sohxlet extraction and liquid-liquid extraction (Husain et al., 1997; Takatsuki et al., 1985), quantified by HPLC. The evaluation results were expressed as the ratio of the experimental group to the control group (Table 3).

[% of control] division naphthalene acenaphthylene fluorene benzopyrene Example 1 13.1 11.5 12.2 9.2 Example 2 14.2 12.3 13.7 10.1 Example 3 13.6 11.9 14.5 9.5 Example 4 11.3 9.6 10.2 7.6 Example 5 9.2 8.2 8.5 7.5 Example 6 10.2 8.9 9.2 8.2 Example 7 9.5 8.1 10.3 7.8 Example 8 6.4 7.7 7.5 6.5 Example 9 8.4 7.6 7.9 7.1 Example 10 9.5 8.3 8.8 7.9 Example 11 9.2 8.0 9.2 7.4 Example 12 6.1 7.4 7.1 6.0 Comparative Example 1 95.3 89.4 94.2 91.2 Comparative Example 2 91.2 85.2 92.6 95.3 Comparative Example 3 92.4 90.1 89.2 89.2 Comparative Example 4 95.9 88.1 87.4 95.3 Comparative Example 5 91.2 95.5 93.4 86.9 Comparative Example 6 85.6 86.4 102.3 87.1 Comparative Example 7 91.2 92.5 98.2 97.4 Comparative Example 8 87.2 89.3 89.3 92.4

Referring to Table 3, the polyaromatic hydrocarbons were significantly reduced by the treatment of the samples of Examples 1 to 12, whereas the samples of Comparative Examples showed little anti-pollution activity.

The above results suggest that the vegetable oil fermented by the Pseudojima strain can effectively remove fine dust and other harmful substances, and the anti-pollution activity can be further enhanced by the addition of self-inflicted herbs.

Experimental Example 3: Fine dust removal effect test

The effect of removing fine dust was tested using the samples of Examples and Comparative Examples.

In order to measure the cleaning power of the fine dust mimetic body, first, the area where the test product was used and the area where the test product was not used (washed with water) were partitioned on the forearm, and pictures were taken.

The fine dust mimic was uniformly applied and the forearm area was photographed with a high-resolution digital camera (DSLR) before and after using the test product.

The skin brightness (intensity value) of the area where the test product was used and the area not used (washed with water) was analyzed using the image analysis program Image pro plus.

It was confirmed that the skin darkened by applying a fine dust mimic was brightened after using the product, and the change in skin intensity value was compared between the water washing area and the area where the test product was used.

Since the skin intensity value and the skin brightness level are proportional, the greater the change rate of the skin intensity value, the better the cleaning power of the fine dust replica. The evaluation results are shown in Table 4 below.

division Fine dust removal rate (%) Example 1 83.6 Example 2 81.3 Example 3 80.5 Example 4 86.6 Example 5 90.6 Example 6 87.0 Example 7 88.0 Example 8 93.1 Example 9 92.7 Example 10 88.1 Example 11 89.8 Example 12 93.9 Comparative Example 1 52.9 Comparative Example 2 50.6 Comparative Example 3 54.9 Comparative Example 4 54.5 Comparative Example 5 54.7 Comparative Example 6 53.0 Comparative Example 7 56.3 Comparative Example 8 56.8 control 52.8

Referring to Table 4, the samples of Examples 1 to 12 effectively removed fine dust.

In particular, the fine dust removal effect of vegetable oils (Examples 5 and 8) fermented by adding self-inflicted vinegar was further improved compared to other examples.

The above results suggest that the vegetable oil fermented by the Pseudojima strain can effectively remove fine dust and other harmful substances, and the anti-pollution activity can be further enhanced by the addition of self-inflicted herbs.

Experimental Example 4: Fine dust adsorption prevention effect test

The effect of preventing adsorption of fine dust was tested using the samples of Examples and Comparative Examples.

Adult women over 30 years of age were exposed to an anti-pollution test device containing fine dust after applying the sample to the forearm.

After applying the fine dust mimetic corresponding to the size of PM2.5 to PM10 in the vacuum chamber under the same conditions, the test site to which the test sample was applied and the test area to which the test sample was not applied were compared through Image-Pro Plus (Table 5).

division Adsorption reduction rate (%) Example 1 44.9 Example 2 43.8 Example 3 42.4 Example 4 49.2 Example 5 56.3 Example 6 54.2 Example 7 53.9 Example 8 64.2 Example 9 58.9 Example 10 55.3 Example 11 55.8 Example 12 67.9 Comparative Example 1 12.2 Comparative Example 2 12.5 Comparative Example 3 16.8 Comparative Example 4 17.3 Comparative Example 5 19.2 Comparative Example 6 10.8 Comparative Example 7 13.8 Comparative Example 8 15.0

Referring to Table 5, in the case of the experimental group to which the samples of Examples 1 to 12 were applied, the reduction in the amount of fine dust adsorption was at a high level, but the vegetable oil of Comparative Example showed little anti-pollution activity.

Experimental Example 5: Antioxidant activity test

The free radical scavenging activity measurement method using the free radical DPPH (1, 1-diphenyl-2-picryl hydrazyl) was used to confirm the free radical decomposition effect.

4 mL methanol was put into a test tube, and samples of Examples and Comparative Examples were added. After adding 1 mL of 0.15 mM DPPH solution and reacting at room temperature for 30 minutes, absorbance was measured at 520 nm.

Initial (Ai) and blank (Ab) without substrate and DPPH were measured, and α-tocopherol and BHT were used as positive controls. The results are shown in Table 6 below.

division DPPH clearance % α-tocopherol 76.5 BHT 71.5 Example 1 44.8 Example 2 42.5 Example 3 43.4 Example 4 48.8 Example 5 81.0 Example 6 79.0 Example 7 78.9 Example 8 86.1 Example 9 83.2 Example 10 79.8 Example 11 81.4 Example 12 87.8 Comparative Example 1 43.9 Comparative Example 2 41.4 Comparative Example 3 43.4 Comparative Example 4 42.2 Comparative Example 5 42.3 Comparative Example 6 43.3 Comparative Example 7 44.4 Comparative Example 8 50.2

Referring to Table 6, in the case of vegetable oils, the antioxidant effect was not significantly increased by fermentation, but in the case of vegetable oils fermented with the addition of self-inflicted herbs (Examples 5 and 8), the antioxidant effect was significantly enhanced.

Vegetable oils (Examples 5 to 12) fermented with the addition of self-inflicted herbs were evaluated to have the same or higher level of free radical scavenging activity than α-tocopherol and BHT, which are well known in the art.

That is, the decomposition of active oxygen can be promoted by the samples of Examples 5 to 12, and the above results suggest that the anti-aging effect and the skin condition improvement effect by the antioxidant activity are excellent.

Experimental Example 6: Anti-inflammatory activity test

The NF-kB transcriptional inhibitory effect was measured using the NF-kB reporter assay. LPS-induced iNOS, COX-2, IFN-β or TNF-α are expressed by the transcription factor NF-kB.

NF-kB is inactivated in a complex state with IkB in the cytoplasm. When I-kB-α is phosphorylated by I-kB-αkinase, the bound complex is decomposed and activated as NF-kB, and it moves into the nucleus (translocation) do.

It induces the expression of the target gene of NF-kB and causes inflammation. TRIF, which acts on inflammatory diseases such as multiple sclerosis, is a TIP-domain having a receptor that induces interferon-β. As a receptor that responds to the activity of toll-like-receptors (TLRs), the TLRs are the invading microorganisms. Recognizes a specific component and activates an immune response to an antigen.

The receptor recognizes a well-conserved pathogenic pattern and activates a signal to stimulate the release of inflammatory cytokines.

IRF-3 (interferon-stimulating gene factor 3) is a transcriptional activator induced by interferon α/β stimulation. It binds to the interferon stimulation response sequence (ISRE) in the transcriptional regulatory region of most interferon-inducing genes and activates its transcription.

β-galactosidase (NF-kB-Luc or TRIF depending on the presence or absence of β-like receptor molecules (MyD88 and TRIF) using the PEI method in 12-well plates according to the manufacturer's protocol (Jin et al., 2009). 1 μg of each containing plasmid was injected (transfection) into HEK293 (1×10 ⁶ cells/ml) cells.

After culturing for 24 hours, the samples of Examples and Comparative Examples were treated with LPS (100 ng/ml), and NF-kB activity and IRF-3 activity were measured using luciferase assays after 24 hours. did.

The luciferase assay was performed using the luciferase assay system (Promega Co., USA) reported in the manufacturer's protocol (Jung et al., 2009), and the analysis results are shown in Table 7 below.

division NF-kB activity IRF-3 activity Control group (blank group) 1.00 1.00 Example 1 0.75 0.80 Example 2 0.77 0.82 Example 3 0.80 0.86 Example 4 0.73 0.78 Example 5 0.65 0.70 Example 6 0.70 0.75 Example 7 0.68 0.73 Example 8 0.58 0.62 Example 9 0.63 0.68 Example 10 0.68 0.74 Example 11 0.67 0.72 Example 12 0.55 0.61 Comparative Example 1 1.21 1.10 Comparative Example 2 1.15 1.05 Comparative Example 3 1.37 1.25 Comparative Example 4 1.29 1.17 Comparative Example 5 1.21 1.10 Comparative Example 6 1.24 1.13 Comparative Example 7 1.34 1.22 Comparative Example 8 1.44 1.31

[Fold, Luciferase activity]

Referring to Table 7, NF-kB activity and IRF-3 activity were significantly reduced by the samples of Examples.

The above results suggest that the samples of Examples 1 to 12 effectively inhibit the transcription of NF-kB, thereby inhibiting or alleviating inflammation and having an effect of preventing and improving inflammatory diseases.

Experimental Example 7: XRE expression inhibition activity test

The expression level of the XRE gene by fine dust (UPM) was measured using a luciferase reporter system.

Measurements were made on a Turner Designs TD 20/20 luminometer (Turner Designs, USA) using the self-developed XRE-Luciferase Reporter Assay System.

100 μL of luciferase reagent (Steady Glo Reagent) was reacted in a culture dish with cells for 5 minutes, each sample was transferred to a 96-well plate, and the fluorescence value was calculated through a fluorescence meter (Luminometer).

The experimental group that was not treated with fine dust (untreated group) was used as a negative control group, and the experimental group treated only with fine dust was used as a positive control group. It was.

division Luciferase activity Example 1 62.3 Example 2 63.9 Example 3 66.4 Example 4 60.6 Example 5 54.0 Example 6 58.1 Example 7 56.4 Example 8 48.1 Example 9 52.1 Example 10 56.7 Example 11 54.4 Example 12 46.5 Comparative Example 1 101.2 Comparative Example 2 102.7 Comparative Example 3 100.3 Comparative Example 4 101.6 Comparative Example 5 102.5 Comparative Example 6 99.3 Comparative Example 7 101.2 Comparative Example 8 98.2 negative control 18.5 positive control 100

Referring to FIG. 1 , sunflower seed oil effectively reduced the expression of XRE by fermentation.

Referring to Table 8, the amount of XRE expression was significantly reduced by the treatment of the samples of Examples 1 to 12, whereas in the case of samples that did not undergo fermentation or had different fermentation microorganisms (Comparative Examples 1 to 8), the expression level of XRE was reduced. couldn't do it

The above results suggest that the sample of Examples can effectively protect the skin from external stimuli through the fermentation process by the Pseudozima sp. strain.

Experimental Example 8: Confirmation of skin improvement activity according to vegetable oil substitution

The sunflower seed oil, olive oil and apricot seed oil used in the experiment were substituted with palm oil, argan oil, green tea seed oil, grape seed oil, meadowfoam seed oil, soybean oil, grape seed oil, sesame oil, and perilla oil, Experimental Example Experiments 1 to 6 were repeated.

As a result of the experiment, although there are some differences in the case of vegetable oil, antioxidant and anti-inflammatory activities were enhanced along with anti-pollution activity by fermentation of the Pseudozima sp. strain.

The above results suggest that the Pseudozima sp. strain can implement beneficial skin improvement activity by changing the active ingredient of vegetable oil through fermentation.

The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.

Name of deposit institution: Korea Research Institute of Bioscience and Biotechnology

Accession number: KCTC08950P

Deposit date : 19990624

Claims (8)

Vegetable oil for preventing or removing skin fine dust fermented by Pseudozima sp . According to claim 1,
The strain is Pseudojima genus SY16 (KCTC 8950P), vegetable oil. The method of claim 1,
A vegetable oil, wherein at least one is selected from the group consisting of sunflower seed oil, olive oil and apricot seed oil. 4. The method of claim 3,
A vegetable oil that is fermented by adding at least one selected from the group consisting of self-inflicted herbs, olive leaves and hemp seeds. According to claim 1,
A vegetable oil, further comprising antioxidant, and anti-inflammatory uses. According to claim 1,
A vegetable oil that inhibits TRPV1 or XRE activated by fine dust. A cosmetic composition comprising the vegetable oil of any one of claims 1 to 6 as an active ingredient. 8. The method of claim 7,
From the group consisting of flexible lotion, nourishing lotion, moisture cream, nourishing cream, massage cream, nourishing lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, cleansing water, mask pack, spray and powder A cosmetic composition formulated with one or more selected.

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