KR20220094440A - light cultivating device for adherent diatoms - Google Patents
light cultivating device for adherent diatoms Download PDFInfo
- Publication number
- KR20220094440A KR20220094440A KR1020200185657A KR20200185657A KR20220094440A KR 20220094440 A KR20220094440 A KR 20220094440A KR 1020200185657 A KR1020200185657 A KR 1020200185657A KR 20200185657 A KR20200185657 A KR 20200185657A KR 20220094440 A KR20220094440 A KR 20220094440A
- Authority
- KR
- South Korea
- Prior art keywords
- light
- light source
- diatoms
- present
- culture
- Prior art date
Links
- 230000001464 adherent effect Effects 0.000 title claims abstract description 19
- 241000206761 Bacillariophyta Species 0.000 title claims description 21
- 239000011941 photocatalyst Substances 0.000 claims abstract description 12
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 238000012258 culturing Methods 0.000 claims description 13
- 229910010413 TiO 2 Inorganic materials 0.000 claims description 11
- 239000013535 sea water Substances 0.000 claims description 11
- 229910052751 metal Inorganic materials 0.000 claims description 9
- 239000002184 metal Substances 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 7
- 230000001678 irradiating effect Effects 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- 241001491724 Melosira nummuloides Species 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 239000010949 copper Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 238000000354 decomposition reaction Methods 0.000 abstract description 2
- 239000011368 organic material Substances 0.000 abstract description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 238000002834 transmittance Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 241001536303 Botryococcus braunii Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- GYHNNYVSQQEPJS-UHFFFAOYSA-N Gallium Chemical compound [Ga] GYHNNYVSQQEPJS-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229910052733 gallium Inorganic materials 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000012770 industrial material Substances 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052754 neon Inorganic materials 0.000 description 1
- GKAOGPIIYCISHV-UHFFFAOYSA-N neon atom Chemical compound [Ne] GKAOGPIIYCISHV-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000001699 photocatalysis Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J35/00—Catalysts, in general, characterised by their form or physical properties
- B01J35/002—Catalysts characterised by their physical properties
- B01J35/004—Photocatalysts
-
- B01J35/39—
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/24—Recirculation of gas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
Abstract
Description
본 발명은 배양용기, 광원, 투광창, 기계적 세척장비, 기포발생기를 포함하는 부착성 규조류 광배양 장치에 관한 발명으로, 더욱 상세하게는 투광창에 규조가 부착됨으로 인한 차광작용에 의하여 배양효율이 급격히 저하되는 문제를 방지하기 위하여 광촉매가 코팅된 투광창을 구비한 부착성 규조류 광배양 장치에 관한 발명이다. The present invention relates to an adherent diatom light culture apparatus including a culture vessel, a light source, a light-transmitting window, a mechanical washing equipment, and a bubble generator. The present invention relates to an adherent diatom photoculture device having a light-transmitting window coated with a photocatalyst in order to prevent the problem of rapid deterioration.
용암해수란 제주도의 현무암층을 뚫고 육지 지하로 흘러 들어온 바닷물이다. 용암해수는 제주도의 서부 일부지역과 동부지역을 중심으로 발견되며 고농도의 미네랄을 함유하고 있다. 제주도 서부 일부지역과 동부지역은 용암이 굳어 형성된 화산암이 지표에서부터 해수면 아래 약 150m 내외까지 두텁게 분포하며, 본암층은 지하수를 저류시킬 수 있는 공극이 차지하는 비율이 높으며, 해안과 인접하여 해수와 약간 의 지하수가 혼합된 대수층이 발달하고 있다. 또한 일반해수는 생활하수, 산업폐수, 항만오염 등의 불안정한 환경에 노출되어 산업화 소재 가공에 많은 비용이 소요되는 반면, 용암해수는 화산암반층에 의한 자연정화와 여과를 거쳐 중금속 흡착 및 유해물질을 차단하기 때문에 안전성과 안정성, 경제성을 확보하고 있고, 깊은 바다에서 취수하는 해양심층수에 비해서 비교할 수 없을 정도로 취수비용이 저렴하다.Lava seawater is seawater that penetrates the basalt layer of Jeju Island and flows underground. Lava seawater is found mainly in the western and eastern parts of Jeju Island and contains high concentrations of minerals. In some western and eastern parts of Jeju Island, volcanic rocks formed by hardening lava are distributed thickly from the surface to about 150m below the sea level. Aquifers mixed with groundwater are developing. In addition, while general seawater is exposed to unstable environments such as domestic sewage, industrial wastewater, and port pollution, it takes a lot of money to process industrial materials, whereas lava seawater undergoes natural purification and filtration by volcanic bedrock to block heavy metal adsorption and harmful substances. Therefore, it secures safety, stability, and economic feasibility, and the cost of water intake is incomparably lower than that of deep sea water taken from the deep sea.
본 출원의 발명자는 제주 용암해수를 이용하여 양식을 하는 과정에서 용암해수와 함께 부착성 규조류가 취수되는 것을 확인하고, 이들 부착성 규조류를 우점시켜 대량 배양하는 방법을 확립하였다. 분리 확인된 부착성 규조류는 양식 산업의 사료 대체 에너지원으로서 또는 의학, 환경, 생명산업 등 여러 산업분야의 기초재로서 가치가 높은 자원으로 활용가능하다.The inventor of the present application confirmed that adherent diatoms were taken together with lava seawater during aquaculture using Jeju lava seawater, and established a method of mass-cultivating these adherent diatoms dominantly. The isolated and confirmed adherent diatoms can be used as a valuable resource as an alternative energy source for feed in the aquaculture industry or as a basic material in various industrial fields such as medicine, environment, and life industry.
이러한 부착성 규조류 대량배양에 있어서, 자연광을 이용하는 경우 초기 설비투자와 운전비용이 저렴한 장점이 있으나, 배양장치 내부로 효과적인 빛 전달이 이루어지지 않아 균체의 성장속도가 느리고, 균체의 성장 수율이 낮으며, 오염으로 인한 미세조류 생물량 및 종의 불안정성, 영양원의 불균등 분포 등의 문제점과 더불어 많은 양의 이산화탄소를 제거하기 위해서는 넓은 설치공간이 필요한 단점이 있다.In the mass culture of adherent diatoms, if natural light is used, the initial equipment investment and operating cost are low, but effective light transmission is not made inside the culture device, so the growth rate of the cells is slow, and the growth yield of the cells is low. In addition to problems such as instability of microalgal biomass and species due to contamination, and uneven distribution of nutrient sources, there is a disadvantage that a large installation space is required to remove a large amount of carbon dioxide.
한편, 광원으로 형광등을 사용할 경우, 광변환효율이 백열등(8%)에 비해서는 높지만 LED(25~30%)에 비해서는 낮으며, 이로 인해 같은 광량을 발산할 경우, 형광등에서의 외부 접촉온도는 110℃에 육박하여 재배식물이나 미세조류 배양기에 가까이 둘 수 없으며, 통상 빛의 세기는 광원과의 거리의 제곱에 반비례하므로 광효율이 급격히 저하되는 문제점이 있었다.On the other hand, when a fluorescent lamp is used as a light source, the light conversion efficiency is higher than that of an incandescent lamp (8%) but lower than that of an LED (25-30%). can not be placed close to cultivated plants or microalgae incubators because it approaches 110°C, and since the intensity of light is inversely proportional to the square of the distance from the light source, there is a problem that the light efficiency is rapidly reduced.
또한, 광원으로 LED를 사용할 경우에도 LED를 둘러싸고 있는 투광창에 규조가 부착되어 차광작용에 의하여 효율이 급격히 저하되는 문제가 있고, 종래 클리닝 장치를 설치하여 이를 해결하고자 하였으나, 빈번한 클리닝은 투광창에 손상을 일으켜 광투과도가 감소하게 되는 문제가 있었다. In addition, even when an LED is used as a light source, diatoms are attached to the light-transmitting window surrounding the LED, and there is a problem that the efficiency is rapidly reduced due to the light-shielding action. There was a problem in that the light transmittance was reduced by causing damage.
종래 부착성 규조류의 배양은 주로 부착기질에 자연적으로 부착된 규조류를 자연배양하거나 유수식으로 배양하여 전복 등의 먹이로 사용하면서 양식생물에게 적합한 최적의 규조류를 선택적으로 부착 및 배양시키기 어려운 점이 있었으며, 부착기질에 적층하며 자라고 탈락하여 죽는 것을 반복하는 생물학적 특성상 대량배양이 용이하지 않았다Conventional culturing of adherent diatoms mainly involves culturing diatoms naturally attached to the adherent substrate in a natural or flowing manner and using them as food such as abalone, while selectively attaching and culturing optimal diatoms suitable for cultured organisms. It was not easy to mass-culture due to the biological nature of repeating growth, dropping, and dying on the substrate.
이러한 부착성 규조류 배양에 있어서, 광원으로 LED를 사용할 경우 LED를 둘러싸고 있는 투광창에 규조가 부착됨으로 인한 차광작용에 의하여 효율이 급격히 저하되는 문제가 있었다. In the culturing of adherent diatoms, when an LED is used as a light source, there is a problem in that the efficiency is rapidly reduced due to the light blocking effect due to the attachment of the diatom to the light-transmitting window surrounding the LED.
상기와 같은 과제를 해결하기 위하여 본 발명은 배양액이 수용되는 배양용기, 상기 배양용기의 내부에서 빛을 조사하는 광원, 상기 광원을 둘러싸며 광촉매가 코팅된 투광창, 상기 투광창 외부에 설치되는 기계적 세척장비, 상기 배양액에 산소를 공급하는 기포발생기를 포함하는 부착성 규조류 광배양 장치를 제공하는 바, 배양용기의 하부에 형성되어 있는 기포발생기는 공기공급장치를 통해 배양용기 외부로부터 공기를 규조류에 공급한다. In order to solve the above problems, the present invention provides a culture vessel in which a culture solution is accommodated, a light source irradiating light from the inside of the culture vessel, a light transmitting window with a photocatalyst coating surrounding the light source, and a mechanical installation outside the light transmitting window There is provided an adhesive diatom photo-cultivation device comprising a washing equipment and a bubble generator for supplying oxygen to the culture medium, wherein the bubble generator formed at the bottom of the culture vessel supplies air from the outside of the culture vessel to the diatoms through an air supply device. supply
또한 본 발명에서 광원은 LED(light emitting diode, 발광다이오드)인 것이 바람직하며, 미세조류의 광합성에 적합한 파장범위의 LED를 선택하거나 백색광을 사용할 수 있다. In addition, the light source in the present invention is preferably an LED (light emitting diode, light emitting diode), it is possible to select an LED of a wavelength range suitable for photosynthesis of microalgae or use white light.
또한 본 발명은 광원의 지지체 역할과 냉각기능을 하는 원형 또는 삭각형 단면의 금속막대를 더 포함하는 것을 특징으로 할 수 있다. In addition, the present invention may be characterized in that it further comprises a metal rod of a circular or angled cross-section serving as a support for the light source and a cooling function.
또한 본 발명은 금속막대의 일단에 냉각핀을 더 포함할 수 있다. In addition, the present invention may further include a cooling fin at one end of the metal rod.
또한 본 발명에서 금속막대의 재질은 구리로 이루어진 것을 특징으로 할 수 있다. In addition, the material of the metal rod in the present invention may be characterized in that it is made of copper.
또한 본 발명에서 광촉매는 TiO2인 것을 특징으로 할 수 있다.In addition, the photocatalyst in the present invention may be characterized in that TiO 2 .
또한 본 발명에서 규조류는 제주 용암해수로부터 분리된 Melosira nummuloides 인 것을 특징으로 할 수 있다. In addition, diatoms in the present invention may be characterized as Melosira nummuloides isolated from Jeju lava seawater.
본 발명에 의한 부착성 규조류 광배양 장치는 투광창에 코팅된 광촉매의 유기물 분해작용에 의하여 투광창의 물리적 세척빈도를 현저히 감소시킴에 따라 투광창 손상을 최소화할 수 있고, 그 결과 부착성 규조의 배양효율을 극대화시킬 수 있다. Adhesive diatom photo-cultivation device according to the present invention can minimize damage to the light-transmitting window by remarkably reducing the frequency of physical washing of the light-transmitting window due to the decomposition of organic matter of the photocatalyst coated on the light-transmitting window, and as a result, culture of adherent diatoms efficiency can be maximized.
도 1은 TiO2로 코팅한 일단부와 아무런 처리가 되지 아니한 타단부의 시간경과에 따른 광 투과도를 비교하여 나타낸 것이다. 1 shows a comparison of the light transmittance over time of one end coated with TiO 2 and the other end that is not treated at all.
본 발명의 일 실시예에서 배양액이 수용되는 배양용기, 상기 배양용기의 내부에서 빛을 조사하는 광원, 상기 광원을 둘러싸며 광촉매가 코팅된 투광창, 상기 투광창 외부에 설치되는 기계적 세척장비, 상기 배양액에 산소를 공급하는 기포발생기를 포함하는 부착성 규조류 광배양 장치를 제공한다.In an embodiment of the present invention, a culture vessel in which the culture solution is accommodated, a light source irradiating light from the inside of the culture vessel, a light-transmitting window surrounded by a photocatalyst coated with a photocatalyst, a mechanical cleaning equipment installed outside the light-transmitting window, the above It provides an adherent diatom light culture device comprising a bubbler for supplying oxygen to the culture medium.
본 발명에 따른 부착성 규조류 광배양 장치는 외부에서 유입되는 미생물의 오염을 방지하는 밀폐형 구조를 가지며 배양용기에서 배양되는 부착성 규조류의 온도와 광 조건을 충족시키기 위해 배양용기에 온도를 조절하는 냉각순환 장치, 광 에너지를 제공하는 광원과 이산화탄소가 포함된 공기를 제공하는 기포발생기를 포함할 수 있다. The optical culturing apparatus for adherent diatoms according to the present invention has a closed structure that prevents contamination of microorganisms introduced from the outside, and cooling that regulates the temperature in the culture vessel to satisfy the temperature and light conditions of adherent diatoms cultured in the culture vessel It may include a circulation device, a light source providing light energy, and a bubbler providing air containing carbon dioxide.
본 발명의 다른 실시예에서 광원은 LED(light emitting diode, 발광다이오드)인 것이 바람직하며, 미세조류의 광합성에 적합한 파장범위의 LED를 선택하거나 백색광을 사용할 수 있다. In another embodiment of the present invention, the light source is preferably a light emitting diode (LED), and an LED having a wavelength range suitable for photosynthesis of microalgae may be selected or white light may be used.
본 발명에 있어서, LED(Light Emitting Diode)는 발광다이오드로, 갈륨, 인, 비소 등을 재료로 한 다이오드로 전류를 흘려 빛을 발산하는 반도체소자를 의미한다. 상기 LED는 텅스텐전구나 네온램프 등 다른 발광 소자와 비교해서 전기로부터 빛의 변환효율이 양호하여 열을 발하지 않고 소형 경량이기 때문에 수명이 긴 장점이 있다.In the present invention, an LED (Light Emitting Diode) is a light emitting diode, and refers to a semiconductor device that emits light by flowing a current through a diode made of gallium, phosphorus, arsenic, or the like. Compared with other light emitting devices such as a tungsten light bulb or a neon lamp, the LED has a long lifespan because it does not generate heat due to good conversion efficiency of light and does not generate heat.
본 발명에서 광원으로부터의 광은 전범위 스펙트럼 광 또는 특정 파장의 광을 포함한다. 일 실시예에서, 광원은 LED를 구동시키기에 적합한 전력공급기를 더 포함하고, 전력공급기는 광 제어기가 추가로 작동하도록 결합되며, 이때 광 제어기는 LED에 의해 방출되는 광의 강도 및 파장을 제어한다. In the present invention, the light from the light source includes a full spectrum light or light of a specific wavelength. In one embodiment, the light source further comprises a power supply suitable for driving the LED, the power supply coupled to further actuate a light controller, wherein the light controller controls the intensity and wavelength of light emitted by the LED.
본 발명의 또 다른 실시예에서 광원의 지지체 역할과 냉각기능을 하는 원형 또는 삭각형 단면의 금속막대를 더 포함할 수 있다.In another embodiment of the present invention, it may further include a metal rod having a circular or angled cross-section serving as a support for the light source and a cooling function.
본 발명의 또 다른 실시예에서 본 발명은 금속막대의 일단에 냉각핀을 더 포함할 수 있다. In another embodiment of the present invention, the present invention may further include a cooling fin at one end of the metal rod.
본 발명의 또 다른 실시예에서 본 발명에서 금속막대의 재질은 구리로 이루어진 것을 특징으로 할 수 있다. In another embodiment of the present invention, the material of the metal rod in the present invention may be characterized in that it is made of copper.
본 발명의 또 다른 실시예에서 본 발명에서 광촉매는 TiO2인 것을 특징으로 할 수 있다.In another embodiment of the present invention, the photocatalyst in the present invention may be characterized as TiO 2 .
본 발명에서 광촉매로 사용되는 TiO2는 n형 반도체로서 자외선(400nm)을 받으면 전자(Electron), 전공대(Electron Hole)가 형성되어 강한 산화력을 가진 하이드록시 라디칼(-OH)과 슈퍼 옥사이드(O2 -)를 생성하고, 이 이 하이드록시 라디칼과 슈퍼 옥사이드가 유기 화합물을 산화 분해시켜 물(H20)과 탄산가스(CO2)로 변화시키게 된다. TiO 2 used as a photocatalyst in the present invention is an n-type semiconductor. When it receives ultraviolet (400 nm), electrons and electron holes are formed, so that hydroxy radicals (-OH) and superoxide (O) with strong oxidizing power are formed. 2 - ) is generated, and this hydroxy radical and superoxide oxidize and decompose the organic compound to change it into water (H 2 0) and carbon dioxide (CO 2 ).
본 발명의 또 다른 실시예에서 본 발명에서 규조류는 제주 용암해수로부터 분리된 Melosira nummuloides 인 것을 특징으로 할 수 있다. In another embodiment of the present invention, the diatoms in the present invention may be characterized as Melosira nummuloides isolated from Jeju lava seawater.
이하, 구체적인 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지 않는 것은 당업계에서 통상의 지식을 가진 자에 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail through specific examples. These examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples.
실시예. 광촉매 코팅의 효과 확인Example. Check the effect of photocatalytic coating
투광창에 코팅된 광촉매의 효과를 확인하기 위하여 광원을 둘러싸고 있는 투광창의 일단부를 TiO2로 코팅한 후 시간의 경과에 따른 투광창의 광 투과정도를 비교하여 도 1에 나타내었다. 도 1에 따르면, 실험개시 후 10일 정도 경과시까지는 TiO2로 코팅한 일단부와 TiO2로 코팅하지 않은 타단부 모두 광 투과도에 있어 큰 차이를 나타내지 아니하였으나, 25일 경과시 TiO2로 코팅한 일단부는 규조류의 부착이 거의 발생하지 아니하여 광 투과도에 큰 변화가 없었으나, TiO2로 코팅하지 아니한 타단부의 경우 규조류의 부착으로 광 투과도가 현저히 저하되는 것을 확인할 수 있었다. 이는 TiO2로 코팅한 일단부의 경우 부착된 유기물이 산화되어 물(H20)과 탄산가스(CO2)로 변화됨에 기인한다. In order to confirm the effect of the photocatalyst coated on the light transmitting window, one end of the light transmitting window surrounding the light source was coated with TiO 2 and the degree of light transmission of the light transmitting window according to the lapse of time was compared and shown in FIG. 1 . According to FIG. 1, until about 10 days from the start of the experiment, neither the one end coated with TiO 2 nor the other end not coated with TiO 2 showed a significant difference in light transmittance, but after 25 days, the coating was coated with TiO 2 At one end, adhesion of diatoms hardly occurred, so there was no significant change in light transmittance. However, in the case of the other end, which was not coated with TiO 2 , it was confirmed that the light transmittance was significantly lowered due to adhesion of diatoms. This is because, in the case of one end coated with TiO 2 , the attached organic material is oxidized and changed into water (H 2 0) and carbon dioxide (CO 2 ).
Claims (7)
상기 배양용기의 내부에서 빛을 조사하는 광원;
상기 광원을 둘러싸며 광촉매가 코팅된 투광창;
상기 투광창 외부에 설치되는 기계적 세척장비;
상기 배양액에 산소를 공급하는 기포발생기;를 포함하는 부착성 규조류 광배양 장치.
a culture vessel in which the culture medium is accommodated;
a light source irradiating light from the inside of the culture vessel;
a transparent window surrounding the light source and coated with a photocatalyst;
mechanical cleaning equipment installed outside the light-transmitting window;
Adherent diatom photo-culture apparatus comprising a; bubble generator for supplying oxygen to the culture medium.
The apparatus according to claim 1, wherein the light source is an LED.
[Claim 2] The apparatus for culturing adherent diatoms according to claim 1, further comprising a metal rod having a circular or angled cross-section serving as a support for a light source and a cooling function.
[4] The apparatus according to claim 3, further comprising a cooling fin at one end of the metal rod.
[4] The apparatus according to claim 3, wherein the metal rod is made of copper.
The apparatus according to claim 1, wherein the photocatalyst is TiO 2 .
[Claim 2] The apparatus for culturing adherent diatoms according to claim 1, wherein the diatoms are Melosira nummuloides isolated from Jeju lava seawater.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020200185657A KR20220094440A (en) | 2020-12-29 | 2020-12-29 | light cultivating device for adherent diatoms |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020200185657A KR20220094440A (en) | 2020-12-29 | 2020-12-29 | light cultivating device for adherent diatoms |
Publications (1)
Publication Number | Publication Date |
---|---|
KR20220094440A true KR20220094440A (en) | 2022-07-06 |
Family
ID=82400202
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020200185657A KR20220094440A (en) | 2020-12-29 | 2020-12-29 | light cultivating device for adherent diatoms |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR20220094440A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115380812A (en) * | 2022-07-07 | 2022-11-25 | 温州大学 | Algae breeding device for algae-eating organisms and breeding method thereof |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20110081909A (en) | 2005-12-09 | 2011-07-14 | 어드밴스드 아날로직 테크놀로지스 인코퍼레이티드 | Isolation structures for semiconductor integrated circuit substrates and methods of forming the same |
KR20120105460A (en) | 2009-12-16 | 2012-09-25 | 도레이 카부시키가이샤 | Method for analyzing rna |
KR20130042496A (en) | 2010-05-20 | 2013-04-26 | 퀄컴 인코포레이티드 | Apparatus and methods for locating, tracking and/or recovering a wireless communication device |
KR101447387B1 (en) | 2013-09-30 | 2014-10-06 | 한국해양대학교 산학협력단 | The effect of various wavelengths of light from light-emitting diodes on the antioxidant system of marine cyanobacteria Synechococcus sp. |
-
2020
- 2020-12-29 KR KR1020200185657A patent/KR20220094440A/en not_active Application Discontinuation
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20110081909A (en) | 2005-12-09 | 2011-07-14 | 어드밴스드 아날로직 테크놀로지스 인코퍼레이티드 | Isolation structures for semiconductor integrated circuit substrates and methods of forming the same |
KR20120105460A (en) | 2009-12-16 | 2012-09-25 | 도레이 카부시키가이샤 | Method for analyzing rna |
KR20130042496A (en) | 2010-05-20 | 2013-04-26 | 퀄컴 인코포레이티드 | Apparatus and methods for locating, tracking and/or recovering a wireless communication device |
KR101447387B1 (en) | 2013-09-30 | 2014-10-06 | 한국해양대학교 산학협력단 | The effect of various wavelengths of light from light-emitting diodes on the antioxidant system of marine cyanobacteria Synechococcus sp. |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115380812A (en) * | 2022-07-07 | 2022-11-25 | 温州大学 | Algae breeding device for algae-eating organisms and breeding method thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20190169564A1 (en) | Methods of culturing microorganisms in non-axenic mixotrophic conditions | |
EP2740349B1 (en) | Algae cultivation method | |
US4952511A (en) | Photobioreactor | |
RS20060002A (en) | Bioreactor | |
KR101489510B1 (en) | Mass production equipment for marine algae by controlling a photosynthetic light source, a deep seaw | |
CN110760439B (en) | Algae cultivation photo-biological reaction kettle and continuous culture reaction system containing same | |
KR101444642B1 (en) | Wastewater Treating Apparatus for Saving Energy Using Microalgae | |
KR101444643B1 (en) | Wastewater Treating Apparatus Using Microalgae | |
KR20150144782A (en) | Reactor with integrated illumination | |
KR101256773B1 (en) | Purification of wastewater and increment of microalgae biomass by microalgae using wavelength selective irradiation of led | |
KR20220094440A (en) | light cultivating device for adherent diatoms | |
EA026158B1 (en) | Method of reducing residual recalcitrant organic pollutants | |
RU2452693C1 (en) | Rotating biological contactor for biochemical water treatment | |
JP7020229B2 (en) | Microalgae culture method and microalgae | |
RU2668162C1 (en) | Method of chlorella microalgae cultivation | |
JP3181237B2 (en) | Microalgae chlorella and method for immobilizing CO2 using microalgae chlorella | |
WO2015126002A1 (en) | Trapezoidal polarized fluorescence bioreactor | |
Plengsakul et al. | Plastic media reduced algal wall-growth of Chlorococcum humicola for the cultivation in internal-loop airlift photobioreactor | |
JP3073908U (en) | Light source for growing photosynthetic bacteria and sewage treatment apparatus provided with the light source | |
CN103936228A (en) | Method for removing tetracycline in water based on high-rate algae pond | |
JP7313832B2 (en) | Method for culturing haptoalgae and apparatus for culturing haptoalgae | |
RU2683522C1 (en) | Method for biological treatment of waste water | |
KR101622936B1 (en) | Apparatus and method for cultivating micro-algae applied ozone oxidation | |
KR101415553B1 (en) | Device for culturing micro algae | |
Starovoytov et al. | The Rationale for the Fish Farms Circulating Water Biological Treatment Intensification |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
E902 | Notification of reason for refusal |