KR20220086366A - Method for enhancing the content of functional ingredients in seeds - Google Patents
Method for enhancing the content of functional ingredients in seeds Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C7/00—Sowing
- A01C7/06—Seeders combined with fertilising apparatus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing carboxylic groups or thio analogues thereof, directly attached by the carbon atom to a cycloaliphatic ring; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C7/00—Fertilisers containing calcium or other cyanamides
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
본 발명은 화학 물질 처리를 통한 새싹작물의 기능성분 함량 증진 방법을 적용하여 새싹작물을 제조하는 방법에 관한 것이다. 본 발명에 따르면 기능성분 함량이 증진된 새싹작물을 얻을 수 있어 다양한 건강식품으로 널리 활용될 수 있다.The present invention relates to a method for producing a sprout crop by applying a method for enhancing the content of functional components of a sprout crop through chemical treatment. According to the present invention, it is possible to obtain a sprout crop with an enhanced content of functional ingredients, which can be widely used as a variety of health foods.
Description
본 발명은 화학 물질 처리를 통한 새싹작물의 기능성분 함량 증진 방법을 적용하여 새싹작물을 제조하는 방법에 관한 것이다. The present invention relates to a method for producing a sprout crop by applying a method for enhancing the content of functional components of a sprout crop through chemical treatment.
새싹작물은 작물 종자를 싹틔워 어린잎, 줄기, 뿌리의 완전한 식물적 특징을 갖춘 어린 콩나물 형태의 작물을 말한다. 옛 선조들은 다양한 새싹작물을 몸에 좋은 약초(藥草)로 활용하여 각종 질병을 예방하고 건강을 개선하였다. 서양에서는 새싹작물을 이용한 역사는 짧지만 건강기능성 물질과 효능에 주목하면서 다양한 건강식품으로 개발하였다. 우리나라에서도 최근 웰빙 영향으로 새싹채소 소비는 증가하고 있으며, 채소라기보다는 건강기능성 식품으로 여기는 분위기이다. 새싹채소는 신선편의 식품시장에서 주로 소비되며 국내 시장규모는 꾸준히 증가하고 있다.Sprout crops refer to crops in the form of young sprouts that have sprouted crop seeds and have complete botanical characteristics of young leaves, stems and roots. Ancient ancestors used various sprouted crops as medicinal herbs that are good for the body to prevent various diseases and improve health. Although the history of using sprout crops is short in the West, it has been developed as a variety of health foods, paying attention to health functional substances and efficacy. In Korea, the consumption of sprouted vegetables is also increasing due to the influence of well-being in recent years, and it is considered as a health functional food rather than a vegetable. Sprout vegetables are mainly consumed in the fresh convenience food market, and the domestic market size is steadily increasing.
새싹작물의 건강기능성 물질 함량이 높다는 연구결과가 알려지면서 다양한 건강식품으로 개발되고 있다. 종자가 발아할 때 효소 등의 활발한 대사작용으로 각종 영양소, 건강 기능성 물질들을 다량 생성한다. 특히, 햇빛과 물을 이용하여 단백질, 지방을 구성하고 아미노산, 지방산부터 알카로이드, 왁스(Wax)류 등까지 비교적 짧은 시간 내에 다양한 물질을 생성할 수 있다. As the results of research that sprout crops contain high content of health functional substances are known, they are being developed as various health foods. When the seed germinates, it produces a large amount of various nutrients and health functional substances through active metabolism such as enzymes. In particular, it is possible to construct proteins and fats using sunlight and water, and to produce various substances from amino acids and fatty acids to alkaloids and waxes within a relatively short time.
따라서, 새싹작물의 수요 증가 원인에 따라 다양한 효능을 가지면서 유효성분 함량이 높은 새싹작물 연구가 요구되는 실정이다.Therefore, research on sprout crops with a high content of active ingredients while having various efficacy is required depending on the cause of the increase in the demand for sprout crops.
이러한 배경하에 본 발명자들은 새싹작물을 건강기능 식품산업 소재로서 개발하기 위해 예의 연구 노력하였다. 그 결과, 화학물질을 직접 분사하는 방식을 통해 짧은 재배 기간 내에서도 인간에게 이로운 유용 대사체 함량이 증진된 새싹작물을 재배할 수 있음을 발견하고 본 발명은 완성하였다. Under this background, the present inventors made intensive research efforts to develop sprout crops as materials for the health functional food industry. As a result, it was discovered that sprout crops with an enhanced content of useful metabolites beneficial to humans can be cultivated even within a short cultivation period through direct spraying of chemicals, and the present invention has been completed.
본 발명의 목적은 화학적 처리를 통한 기능성분 함량이 증진된 새싹작물 제조방법을 제공하는 것이다.It is an object of the present invention to provide a method for producing sprout crops with enhanced functional ingredient content through chemical treatment.
본 발명의 다른 목적은 상기 제조방법을 통해 제조된 기능성분 함량이 증진된 새싹작물을 제공하는 것이다.Another object of the present invention is to provide a sprout crop with enhanced content of functional ingredients prepared through the above manufacturing method.
본 발명의 또다른 목적은 새싹작물의 기능성분 함량을 증진시키는 방법을 제공하는 것이다. Another object of the present invention is to provide a method for enhancing the functional component content of sprout crops.
도 1을 참조하면, 본 발명의 제1 양태는 침지한 종자를 수득하는 제1 단계; 상기 침지한 종자를 발아시켜 발아한 종자를 수득하는 제2 단계; 및 상기 발아한 종자에 화학 물질을 처리하여 새싹을 재배하는 제3 단계를 포함하는 기능성분 함량이 증진된 새싹작물 제조방법을 제공한다.Referring to Figure 1, a first aspect of the present invention is a first step of obtaining an immersed seed; a second step of germinating the immersed seeds to obtain germinated seeds; and a third step of cultivating sprouts by treating the germinated seeds with chemical substances.
본 발명의 용어 "새싹작물"이란 종자가 발아한 후 7~30일 정도 자라 잎과 뿌리를 수확하는 상태의 작물을 의미한다. 잎을 이용하는 작물로는 새싹보리ㆍ새싹밀ㆍ새싹귀리 등이 있으며, 뿌리를 이용하는 작물은 콩나물ㆍ숙주나물ㆍ새싹땅콩 등이 있고, 잎과 줄기 모두 이용하는 작물은 메밀ㆍ무ㆍ다채ㆍ베이비채소류 등이 있다.As used herein, the term “sprout crop” refers to a crop in which leaves and roots are harvested after the seed germinates and grows for 7 to 30 days. Crops that use leaves include sprouted barley, sprouted wheat, and sprouted oats, while those that use roots include bean sprouts, host greens, and sprouted peanuts. Crops using both leaves and stems include buckwheat, radish, vegetables, and baby vegetables. There is this.
본 발명의 상기 새싹작물은 보리싹, 밀싹, 메밀싹, 무싹, 다채싹 및 귀리싹으로 이루어진 군으로부터 선택된 적어도 하나인 것일 수 있다. 종자 내 탄수화물과 단백질 등은 종자가 발아하고 성장하는 과정에서 일어나는 다양한 효소의 대사작용에 필요한 에너지를 공급한다. 이 과정에서 새싹작물은 각종 필수아미노산, 미네랄 등 영양소와 작물의 종류에 따라 폴리코사놀류, 플라보노이드 배당체류, 사포닌류 등 새싹작물 고유의 건강기능성 물질을 다량 생성하게 된다. The sprout crops of the present invention may be at least one selected from the group consisting of barley sprouts, wheat sprouts, buckwheat sprouts, radish sprouts, multi-chaed sprouts, and oat sprouts. Carbohydrates and proteins in seeds provide the energy necessary for the metabolism of various enzymes that occur during the process of seed germination and growth. In this process, sprout crops produce a large amount of health functional substances unique to sprout crops, such as policosanols, flavonoid glycosides, and saponins, depending on the type of crop and nutrients such as various essential amino acids and minerals.
본 발명의 용어 "기능성분"이란 종자 내에 함유되어 있는 기능성을 나타내는 성분을 의미한다. As used herein, the term "functional ingredient" refers to a component exhibiting functionality contained in seeds.
본 발명의 상기 기능성분은 폴리코사놀 또는 플라보노이드인 것일 수 있다. 상기 폴리코사놀은 구체적으로 옥타코사놀, 헥사코사놀일 수 있다. 상기 플라보노이드는 구체적으로 플라본글루코사이드는 보다 구체적으로 아이소오리엔틴 또는 아이소샤프토사이드인 것일 수 있다. The functional component of the present invention may be policosanol or a flavonoid. The policosanol may be specifically octacosanol or hexacosanol. Specifically, the flavonoid may be a flavone glucoside, more specifically isoorientin or isoshaftoside.
상기 폴리코사놀은 식물 왁스로부터 추출 가능한 천연 지방 알코올의 한 종류로 콜레스테롤 특히 LDL을 낮춰 혈관 질환 예방 또는 개선에 효과가 있는 것으로 알려져 있다.The policosanol is a type of natural fatty alcohol that can be extracted from plant wax and is known to be effective in preventing or improving vascular diseases by lowering cholesterol, particularly LDL.
상기 플라보노이드는 폴리페놀에 속하는 수용성 식물색소로, 항산화 작용과 모세 혈관을 강화시키는 효과가 있는 것으로 알려져 있다. 상기 아이소오리엔틴 및 아이소샤프토사이드는 플라보노이드의 당 부분구조를 의미한다. The flavonoids are water-soluble plant pigments belonging to polyphenols, and are known to have an antioxidant action and an effect of strengthening capillaries. The isoorientin and isoshaftoside refer to the sugar moiety of flavonoids.
특히 아이소오리엔틴 또는 아이소샤프토사이드는 항산화, 항염, 피부조직증식, 또는 항알러지 효과를 갖는 것으로 알려져 있다. In particular, isoorientin or isoshaftoside is known to have antioxidant, anti-inflammatory, skin tissue proliferation, or anti-allergic effects.
본 발명의 상기 제1 단계는 종자를 발아 시키기 위한 전처리 단계를 의미한다. 이는 발아시 필요한 수분을 충분히 공급하여 종자 내 효소 활성을 증대시키고 배를 신장시키기 위한 단계로, 물에 12시간 이상 36시간 이하로 침지시킬 수 있다.The first step of the present invention refers to a pre-treatment step for germination of seeds. This is a step for increasing the enzyme activity in the seed and extending the embryo by supplying sufficient moisture for germination, and can be immersed in water for 12 hours or more and 36 hours or less.
상기 침지의 온도 조건은 20
다음으로, 상기 제2 단계는 수분이 흡수된 종자를 발아시키는 단계로 구체적으로 12시간 내지 24시간동안 암조건에서 종피의 파괴 및 유아, 유근이 출현하는 상태에 도달하는 단계를 의미한다.Next, the second step is a step of germinating the seed that has absorbed moisture, and specifically refers to the step of reaching a state in which the seed coat is destroyed and infants and young roots appear under dark conditions for 12 to 24 hours.
다음으로, 상기 제3 단계는 발아한 종자의 재배 과정에서 인간에게 이로운 유용 대사체인 기능성분의 함량을 증가시키기 위한 화학 물질을 처리하는 단계를 의미한다. 상기 제3 단계를 통해 건강기능 식품으로서 요구되는 새싹작물의 기능성을 향상시킬 수 있다.Next, the third step refers to a step of treating a chemical substance to increase the content of a functional component that is a useful metabolite beneficial to humans in the process of cultivating the germinated seed. Through the third step, it is possible to improve the functionality of the sprout crop required as a health functional food.
상기 화학 물질은 염 형태 또는 유기화합물 형태인 것일 수 있다. 구체적으로 염 형태의 화학 물질은 CaCl2(염화칼슘), NaCl(염화나트륨), K2SO4(탄산칼륨), Na2CO3(탄산나트륨) 및 Ca3PO4(인산칼슘)일 수 있고, 유기화합물 형태의 화학 물질은 메틸자스모네이트일 수 있다. The chemical substance may be in the form of a salt or an organic compound. Specifically, the chemical substance in the form of a salt may be CaCl 2 (calcium chloride), NaCl (sodium chloride), K 2 SO 4 (potassium carbonate), Na 2 CO 3 (sodium carbonate) and Ca 3 PO 4 (calcium phosphate), and an organic compound The chemical in the form may be methyljasmonate.
보다 구체적으로, 본 발명의 상기 화학 물질은 CaCl2(염화칼슘), NaCl(염화나트륨), K2SO4(탄산칼륨), Na2CO3(탄산나트륨), Ca3PO4(인산칼슘) 및 메틸자스모네이트로 이루어진 군에서 선택된 적어도 하나인 것일 수 있다.More specifically, the chemical substance of the present invention is CaCl 2 (calcium chloride), NaCl (sodium chloride), K 2 SO 4 (potassium carbonate), Na 2 CO 3 (sodium carbonate), Ca 3 PO 4 (calcium phosphate) and methyl It may be at least one selected from the group consisting of smonate.
상기 메틸자스모네이트는 종자 발아, 식물 방어, 뿌리 성장, 개화, 과일 숙성 및 노화와 같은 다양한 발달 경로에 사용되는 휘발성 유기 화합물을 의미한다. The methyl jasmonate refers to a volatile organic compound used in various developmental pathways such as seed germination, plant defense, root growth, flowering, fruit ripening and aging.
상기 화학물질인 염(salt)의 종류 (CaCl2(염화칼슘), NaCl(염화나트륨), K2SO4(탄산칼륨), Na2CO3(탄산나트륨), Ca3PO4(인산칼슘))는 종자의 발아 및 새싹의 생육 시 고농도로 처리될 경우 피해를 많이 보지만, 극소량(4~15mM)의 처리에 따르면 생장속도 증가, 유용대사체 증가, 새싹의 수유 증가 등 다양한 경로에 의해 사용되는 염 화합물을 의미한다. The type of salt (CaCl 2 (calcium chloride), NaCl (sodium chloride), K 2 SO 4 (potassium carbonate), Na 2 CO 3 (sodium carbonate), Ca 3 PO 4 (calcium phosphate)) In the case of germination and growth of sprouts, it causes a lot of damage when treated at high concentrations, but when treated in a very small amount (4~15mM), it means a salt compound used by various routes such as an increase in growth rate, an increase in useful metabolites, and an increase in sprout feeding. do.
상기 화학 물질은 발아한 종자에 직접 분사하는 방식으로 수행되는 것일 수 있다. 구체적으로 상기 화학 물질을 수용액 형태로 제조하여 발아한 종자 표면 및 생장한 새싹에 분사하는 것일 수 있다. 분사 거리는 5cm (단위) 내지 15cm (단위)일 수 있다. 분사 시 압축분무기를 이용하여 분사하였다. The chemical may be directly sprayed onto germinated seeds. Specifically, the chemical substance may be prepared in the form of an aqueous solution and sprayed on the surface of the germinated seed and the sprout. The injection distance may be 5 cm (units) to 15 cm (units). When spraying, it was sprayed using a compressed sprayer.
상기 화학 물질은 1일당 2회 내지 3회 처리할 수 있으며, 1회당 6시간 내지 8시간 간격으로 처리할 수 있다. 6 시간 미만 간격으로 처리할 경우 생육속도 저하 및 종자 부패 문제가 발생할 수 있고, 8 시간 초과 간격으로 처리할 경우 생육속도 및 새싹 수확 후 수율이 현저하게 떨어질 수 있는 문제가 발생할 수 있다.The chemical substance may be treated 2 to 3 times per day, and may be treated at intervals of 6 to 8 hours per time. If the treatment is performed at intervals of less than 6 hours, problems of lowering the growth rate and seed rot may occur.
상기 화학 물질이 메틸자스모네이트인 경우, 농도는 15 ppm 내지 40 ppm 인 것일 수 있다. 메틸자스모네이트는 40 ppm 초과일 경우 플라보노이드 증가량이 40 ppm 미만인 경우보다 감소한다. 또한, 15 ppm 미만일 경우 플라보노이드 증가 효과가 발생하지 않는다. 상기 메틸자스모네이트의 1회당 분사량은 50 mL/min 내지 100 mL/min 인 것일 수 있다.When the chemical substance is methyl jasmonate, the concentration may be 15 ppm to 40 ppm. For methyljasmonate, the amount of increase in flavonoids is reduced when it is more than 40 ppm than when it is less than 40 ppm. In addition, when it is less than 15 ppm, the effect of increasing flavonoids does not occur. The injection amount per one time of the methyl jasmonate may be 50 mL/min to 100 mL/min.
상기 화학 물질이 염 형태의 화학물질로서 CaCl2(염화칼슘), NaCl(염화나트륨), K2SO4(탄산칼륨), Na2CO3(탄산나트륨) 및 Ca3PO4(인산칼슘)으로 이루어진 군에서 선택된 적어도 하나인 경우, 농도는 4 mM 내지 15 mM 인 것일 수 있다. 상기 염 형태의 화학물질의 1회당 분사량은 50 mL/min 내지 100 mL/min 인 것일 수 있다.In the group consisting of CaCl 2 (calcium chloride), NaCl (sodium chloride), K 2 SO 4 (potassium carbonate), Na 2 CO 3 (sodium carbonate) and Ca 3 PO 4 (calcium phosphate) as a chemical substance in salt form In the case of at least one selected, the concentration may be 4 mM to 15 mM. The injection amount per one time of the chemical in the salt form may be 50 mL/min to 100 mL/min.
상기 제3 단계는 재배 온도가 15
본 발명의 제2 양태는 상기 제1 양태의 제조방법으로 제조된 기능성분 함량이 증진된 새싹작물을 제공한다.A second aspect of the present invention provides a sprout crop with enhanced functional ingredient content prepared by the manufacturing method of the first aspect.
상기 용어"기능성분"및 "새싹작물"이란, 상기에서 전술한 바와 같다. The terms "functional ingredient" and "sprout crop" are the same as described above.
상기 제1 양태의 제조방법에 의할 경우, 공지된 기능성분 그 중에서도 폴리코사놀 또는 플라보노이드 함량이 증가된 새싹작물을 재배할 수 있음에 본 발명의 특징이 있다.According to the manufacturing method of the first aspect, there is a feature of the present invention that sprout crops with an increased content of policosanol or flavonoids among known functional ingredients can be grown.
본 발명의 제3 양태는 침지한 종자를 수득하는 제1 단계; 상기 침지한 종자를 발아시켜 발아한 종자를 수득하는 제2 단계; 및 상기 발아한 종자에 화학 물질을 처리하여 새싹을 재배하는 제3 단계를 포함하는 새싹작물의 기능성분 함량 증진 방법을 제공한다.A third aspect of the present invention is a first step of obtaining an immersed seed; a second step of germinating the immersed seeds to obtain germinated seeds; and a third step of cultivating sprouts by treating the germinated seeds with chemical substances.
상기 용어"기능성분"및 "새싹작물"이란, 상기에서 전술한 바와 같다.The terms "functional ingredient" and "sprout crop" are the same as described above.
상기 제1 단계 내지 제3 단계는 상기에서 전술한 바와 같다.The first to third steps are the same as described above.
새싹작물이 함유한 기능성분 중 폴리코사놀 함량 및 플라보노이드 함량을 증진시키고자 할 경우 다양한 종류의 발아 종자에 화학 물질을 처리하는 방법을 적용할 수 있다.If you want to increase the policosanol content and flavonoid content among the functional ingredients contained in sprout crops, you can apply a chemical treatment method to various types of germinated seeds.
본 발명의 화학 물질 처리에 의하면 폴리코사놀 함량이 증진된 새싹작물을 재배할 수 있다.According to the chemical treatment of the present invention, sprout crops with enhanced policosanol content can be grown.
본 발명의 화학 물질 처리에 의하면 플라보노이드 함량이 증진된 새싹작물을 재배할 수 있다. According to the chemical treatment of the present invention, it is possible to cultivate sprout crops with enhanced flavonoid content.
도 1은 본 발명의 제조방법 중 새싹밀에 화학 물질을 처리하는 단계를 이미지화 시킨 도이다.
도 2는 폴리코사놀 성분을 질량 분석기로 분석한 그래프이다.
도 3은 본 발명의 일 실시예에서 제조한 새싹밀의 처리한 염 종류별 폴리코사놀(옥타코사놀) 함량을 비교 분석한 막대 그래프이다.
도 4는 본 발명의 일 실시예에서 제조한 새싹보리의 메틸자스모네이트 처리 농도별 폴리코사놀(헥사코사놀) 함량을 비교 분석한 막대 그래프이다.
도 5는 플라보노이드 중 플라본글루코사이드의 성분을 UPLC(ultra performance liquid chromatography)로 분석한 그래프이다.
도 6은 본 발명의 일 실시예에서 제조한 새싹밀의 처리한 재배 온도 조건별 및 염 종류별 플라본글루코사이드 함량을 비교 분석한 막대 그래프이다.1 is an image of the step of treating the chemical substances in sprout wheat in the manufacturing method of the present invention.
2 is a graph of analysis of the policosanol component with a mass spectrometer.
3 is a bar graph comparing and analyzing the policosanol (octacosanol) content of each treated salt type of sprout wheat prepared in an embodiment of the present invention.
4 is a bar graph comparing and analyzing the policosanol (hexacosanol) content for each methyl jasmonate treatment concentration of sprouted barley prepared in an Example of the present invention.
5 is a graph analyzing the components of flavone glucoside among flavonoids by ultra performance liquid chromatography (UPLC).
6 is a bar graph comparing and analyzing the flavone glucoside content according to the treated cultivation temperature conditions and salt types of sprout wheat prepared in an embodiment of the present invention.
이하, 본 발명의 실시예를 통하여 본 발명을 더욱 상세하게 설명한다. 그러나 이들 실시예는 본 발명을 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail through examples of the present invention. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.
실시예 1 내지 실시예 5. 염 수용액 처리를 통한 새싹작물 재배Examples 1 to 5. Cultivation of sprout crops through salt aqueous solution treatment
실시예 1.1 내지 실시예 5.1: 온실 조건에서 재배할 경우Examples 1.1 to 5.1: Cultivation in greenhouse conditions
새싹밀 종자를 24시간 동안 온도 18~25℃에서 침지한 후, 18시간 이상 암조건에서 발아를 시킨다. 발아시킨 종자는 약 20℃ 온실 조건에서 물빠짐이 좋은 메쉬망에 골고루 뿌려놓고 CaCl2(실시예 1.1), NaCl(실시예 2.1), K2SO4(실시예 3.1), Na2CO-3(실시예 4.1), Ca3PO4(실시예 5.1)으로 총 5가지를 사용하였으며, 각각 처리 농도는 7.5 mM로 동일 농도로 처리하였다. 처리방법은 분사기에 각각의 염 수용액을 담아 새싹에 직접 분사하였다. 10일 내지 14일 경과 후 약 15 cm의 새싹밀을 수득하였다.Sprout wheat seeds are immersed at a temperature of 18 to 25°C for 24 hours, and then germinated in dark conditions for 18 hours or more. The germinated seeds are spread evenly on a mesh network with good drainage in a greenhouse condition of about 20 ° C. CaCl 2 (Example 1.1), NaCl (Example 2.1), K 2 SO 4 (Example 3.1), Na 2 CO -3 (Example 4.1) and Ca 3 PO 4 (Example 5.1) were used in a total of five, each of which was treated at the same concentration as 7.5 mM. As for the treatment method, each salt aqueous solution was put in a sprayer and sprayed directly on the sprout. After 10 to 14 days, sprouted wheat of about 15 cm was obtained.
실시예 1.2 내지 실시예 5.2: 생장상 조건에서 재배할 경우Examples 1.2 to 5.2: When grown under growth conditions
상기 실시예 1.1 내지 실시예 5.1과 CaCl2(실시예 1.2), NaCl(실시예 2.2), K2SO4(실시예 3.2), Na2CO-3(실시예 4.2), Ca3PO4(실시예 5.2)의 각각의 염 수용액 처리를 약 18℃의 생장상 조건에서 재배한 것을 제외하고 상기 실시 예 1.1 내지 실시 예 5.1과 동일하게 새싹밀을 재배하였다.Examples 1.1 to 5.1 and CaCl 2 (Example 1.2), NaCl (Example 2.2), K 2 SO 4 (Example 3.2), Na 2 CO -3 (Example 4.2), Ca 3 PO 4 ( Sprout wheat was grown in the same manner as in Examples 1.1 to 5.1, except that each of the aqueous salt solutions of Example 5.2) was grown under the growth conditions of about 18°C.
실시예 6. 메틸자스모네이트 처리를 통한 새싹작물 재배Example 6. Cultivation of sprout crops through methyl jasmonate treatment
새싹보리 종자를 24시간 동안 온도 18~25℃에서 침지 한 후, 18시간 이상 암조건에서 발아를 시킨다. 발아시킨 종자는 물빠짐이 좋은 메쉬망에 골고루 뿌려놓고 약 20℃ 온실 조건 조건에서 메틸자스모네이트 농도 각각 25 ppm의 식물성장호르몬을 처리하였다. 10일 내지 14일 경과 후 약 15 cm의 새싹보리를 수득하였다.After immersing barley sprout seeds at a temperature of 18~25℃ for 24 hours, let them germinate in dark conditions for more than 18 hours. The germinated seeds were spread evenly on a mesh with good drainage and treated with plant growth hormone at a concentration of 25 ppm of methyl jasmonate, respectively, in a greenhouse condition of about 20 °C. After 10 to 14 days, sprouted barley of about 15 cm was obtained.
비교예 1. 염 수용액 무처리의 새싹작물 재배Comparative Example 1. Cultivation of sprout crops without treatment with aqueous salt solution
새싹밀 종자를 24시간 동안 침지 한 후, 18시간 이상 암조건에서 발아를 시킨다. 발아시킨 종자는 물빠짐이 좋은 메쉬망에 골고루 뿌려놓고 10일 내지 14일 경과 후 약 15 cm 새싹밀을 수득하였다.After soaking sprouted wheat seeds for 24 hours, germinate under dark conditions for 18 hours or more. The germinated seeds were spread evenly on a mesh with good drainage, and after 10 to 14 days, about 15 cm sprouted wheat was obtained.
비교예 2. 메틸자스모네이트 무처리의 새싹작물 재배Comparative Example 2. Cultivation of sprout crops without methyl jasmonate treatment
새싹보리 종자를 24시간 동안 침지 한 후, 18시간 이상 암조건에서 발아를 시킨다. 발아시킨 종자는 물빠짐이 좋은 메쉬망에 골고루 뿌려놓고 10일 내지 14일 경과 후 약 15cm 의 새싹보리를 수득하였다.After immersing sprouted barley seeds for 24 hours, germinate under dark conditions for 18 hours or more. The germinated seeds were spread evenly on a mesh with good drainage, and after 10 to 14 days, sprouted barley of about 15 cm was obtained.
비교예 3.Comparative Example 3. 메틸자스모네이트 고농도 처리를 통한 새싹작물 재배Cultivation of sprout crops through high-concentration treatment of methyl jasmonate
상기 실시예 6에서 메틸자스모네이트 농도 50 ppm 을 제외하고 동일한 방법으로 새싹보리를 재배하였다. Barley sprouts were grown in the same manner as in Example 6, except that the concentration of methyl jasmonate was 50 ppm.
실험예 1. 새싹작물의 폴리코사놀 성분 분석Experimental Example 1. Analysis of policosanol components of sprout crops
상기 실시예 1 내지 6 및 비교예 1 내지 3에서 재배한 새싹밀 또는 새싹보리는 50
도 2는 폴리코사놀 분석 크로마토그램 결과로 이를 참조하면, 헥사코사놀 및 옥타코사놀을 확인할 수 있다. 참고로, 폴리코사놀 분석 기기의 조건은 하기 표 1에 요약하였다.2 is a policosanol analysis chromatogram. Referring to this, hexacosanol and octacosanol can be confirmed. For reference, the conditions of the policosanol analysis instrument are summarized in Table 1 below.
도 3에 나타낸 바와 같이, 새싹밀 재배시 염의 종류별로 상기 비교예 1, 실시예 1.1 내지 5.1 및 상기 실시예 1.2 내지 5.2와 같이 처리한 후 폴리코사놀 중 옥타코사놀 함량 변화를 분석하였다. 염 수용액 처리시 물만 처리한 비교예 1의 새싹밀보다 친수성 유용 대사체인 옥타코사놀 함량이 높게 나타남을 알 수 있었다. 특히, 실시예 1.1, 실시예 4.2 및 실시예 5.2 의 옥타코사놀 함량 증가가 매우 높은 것을 확인하였다.As shown in FIG. 3 , changes in octacosanol content in policosanol were analyzed after treatment as in Comparative Example 1, Examples 1.1 to 5.1, and Examples 1.2 to 5.2 for each type of salt during sprout wheat cultivation. It was found that the content of octacosanol, a hydrophilic useful metabolite, was higher than that of the sprout wheat of Comparative Example 1 treated with only water when the salt aqueous solution was treated. In particular, it was confirmed that the increase in the octacosanol content of Examples 1.1, 4.2 and 5.2 was very high.
도 4에 나타낸 바와 같이, 새싹보리 재배시 메틸자스모네이트 농도별로 상기 비교예 2, 비교예 3 및 실시예 6과 같이 처리한 후 폴리코사놀 중 헥사코사놀 함량 변화를 분석하였다. 메틸자스모네이트 무처리한 비교예 2 의 새싹보리에 비해 25 ppm(실시예 6) 및 50 ppm(비교예 3)의 헥사코사놀 함량이 증가하는 것을 알 수 있었다. 그러나, 실시예 6의 저농도(25ppm)로 처리하였을 경우 비교예 3의 고농도(50ppm)로 처리할 때보다 더 높은 헥사코사놀 함량을 확인함으로써 폴리코사놀 함량을 최대로 증가시킬 수 있는 유의미한 농도 범위가 존재함을 확인하였다.As shown in FIG. 4 , changes in hexacosanol content in policosanol were analyzed after treatment as in Comparative Examples 2, 3 and 6 for each methyl jasmonate concentration during cultivation of sprouted barley. It was found that the hexacosanol content of 25 ppm (Example 6) and 50 ppm (Comparative Example 3) was increased compared to the sprouted barley of Comparative Example 2 that was not treated with methyl jasmonate. However, when treated with a low concentration (25 ppm) of Example 6, by confirming a higher hexacosanol content than when treated with a high concentration (50 ppm) of Comparative Example 3, there is a significant concentration range capable of maximally increasing the policosanol content. was confirmed.
실험예 2.Experimental Example 2. 새싹작물의 플라보노이드 성분 분석Analysis of Flavonoid Components of Sprouts
상기 실시예 1 내지 실시예 5 및 비교예 1에서 재배한 새싹밀은 50
플라보노이드 분석 기기의 조건은 하기 표 2에 요약하였다.The conditions of the flavonoid assay instrument are summarized in Table 2 below.
도 5에 나타낸 바와 같이, UPLC 분석을 통해 플라보노이드 중 아이소오리엔틴의 고유 피크(peak) 및 아이소샤프토사이드의 고유 피크값을 확인할 수 있었다. 이를 기준으로 본 발명의 일 실시예에서 재배한 새싹밀의 성분 중 플라보노이드 성분을 확인할 수 있었다. As shown in FIG. 5 , the intrinsic peak value of isoorientin and the intrinsic peak value of isoshaftoside among flavonoids could be confirmed through UPLC analysis. Based on this, it was possible to confirm the flavonoid component among the components of sprout wheat grown in one embodiment of the present invention.
도 6에 나타낸 바와 같이, 온실 조건(실시예 1.1 내지 5.1 및 비교예 1) 또는 생장상 조건(실시예 1.2 내지 5.2 및 비교예 1)을 달리하여 재배한 새싹밀의 플라보노이드 성분 분석시 대부분의 염 처리시 물만 처리한 새싹밀보다 친수성 유용대사체인 플라본글루코사이드 함량이 높게 나타났다. 특히 Na2CO3 및 Ca3PO4 처리 시 친수성 성분인 플라본글루코사이드 함량이 높게 나타났으며, 생장상 및 온실 조건 모두에서 비교예 1과 비교해 플라본글루코사이드 함량이 증가하였으나, 생장상보다는 온실에서 재배한 것이 더 높은 함량 증가가 있는 것을 알 수 있었다.As shown in FIG. 6, most salt treatment in the analysis of flavonoid components of sprouted wheat grown under different greenhouse conditions (Examples 1.1 to 5.1 and Comparative Example 1) or growth conditions (Examples 1.2 to 5.2 and Comparative Example 1) The content of flavone glucoside, a hydrophilic useful metabolite, was higher than that of sprout wheat treated with only water. In particular, during treatment with Na 2 CO 3 and Ca 3 PO 4 , the content of flavone glucoside, a hydrophilic component, was high, and the content of flavone glucoside was increased compared to Comparative Example 1 in both growth phase and greenhouse conditions. It was found that there was a higher content increase.
이상의 설명으로부터, 본 발명이 속하는 기술분야의 당업자는 본 발명이 그 기술적 사상이나 필수적 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 이와 관련하여, 이상에서 기술한 실시 예들은 모든 면에서 예시적인 것이며 한정적인 것이 아닌 것으로서 이해해야만 한다. 본 발명의 범위는 상기 상세한 설명보다는 후술하는 특허 청구범위의 의미 및 범위 그리고 그 등가 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims to be described later and their equivalents.
Claims (11)
상기 침지한 종자를 발아시켜 발아한 종자를 수득하는 제2 단계; 및
상기 발아한 종자에 화학 물질을 처리하여 새싹을 재배하는 제3 단계를 포함하는, 기능성분 함량이 증진된 새싹작물 제조방법.
A first step of obtaining immersed seeds;
a second step of germinating the immersed seeds to obtain germinated seeds; and
A method for producing sprout crops with enhanced functional ingredient content, comprising a third step of cultivating sprouts by treating the germinated seeds with chemical substances.
상기 제3 단계에서 상기 화학 물질은 CaCl2(염화칼슘), NaCl(염화나트륨), K2SO4(탄산칼륨), Na2CO3(탄산나트륨), Ca3PO4(인산칼슘) 및 메틸자스모네이트로 이루어진 군에서 선택된 적어도 하나인 것인, 기능성분 함량이 증진된 새싹작물 제조방법.
According to claim 1,
In the third step, the chemical substances are CaCl 2 (calcium chloride), NaCl (sodium chloride), K 2 SO 4 (potassium carbonate), Na 2 CO 3 (sodium carbonate), Ca 3 PO 4 (calcium phosphate) and methyljasmonate. At least one selected from the group consisting of, a method for producing sprout crops with enhanced functional ingredient content.
상기 제3 단계는 상기 화학 물질을 발아한 종자에 직접 분사하는 방식으로 수행되는, 기능성분 함량이 증진된 새싹작물 제조방법.
According to claim 1,
The third step is a method for producing sprout crops with enhanced functional ingredient content, which is performed by directly spraying the chemical substance onto germinated seeds.
새싹작물은 보리싹, 밀싹 및 귀리싹으로 이루어진 군으로부터 선택된 적어도 하나인 것인, 기능성분 함량이 증진된 새싹작물 제조방법.
According to claim 1,
The sprout crop is at least one selected from the group consisting of barley sprouts, wheat sprouts and oat sprouts, a method for producing sprout crops with enhanced functional ingredient content.
기능성분은 폴리코사놀 또는 플라보노이드인 것인, 기능성분 함량이 증진된 새싹작물 제조방법.
According to claim 1,
The functional ingredient is policosanol or a flavonoid, a method for producing sprout crops with enhanced functional ingredient content.
상기 화학 물질이 메틸자스모네이트인 경우, 농도는 15 ppm 내지 40 ppm 인 것인, 기능성분 함량이 증진된 새싹작물 제조방법.
3. The method of claim 2,
When the chemical substance is methyl jasmonate, the concentration is 15 ppm to 40 ppm, the functional ingredient content is enhanced sprouting crop production method.
상기 제3 단계는 재배 온도가 15
The method of claim 1,
In the third step, the cultivation temperature is 15
상기 제3 단계에서 상기 화학 물질은 1일당 1회 내지 3회 처리하는, 기능성분 함량이 증진된 새싹작물 제조방법.
According to claim 1,
In the third step, the chemical substance is treated 1 to 3 times per day, the functional ingredient content is enhanced sprout crop production method.
상기 화학 물질은 1회당 6 시간 내지 8시간 간격으로 처리하는, 기능성분 함량이 증진된 새싹작물 제조방법.
9. The method of claim 8,
The chemical substance is treated at intervals of 6 to 8 hours per time, the functional ingredient content is enhanced sprout crop production method.
A sprout crop with an enhanced functional ingredient content prepared by the method of any one of claims 1 to 9.
상기 침지한 종자를 발아시켜 발아한 종자를 수득하는 제2 단계; 및
상기 발아한 종자에 화학 물질을 처리하여 새싹을 재배하는 제3 단계를 포함하는, 새싹작물의 기능성분 함량 증진 방법.
A first step of obtaining immersed seeds;
a second step of germinating the immersed seeds to obtain germinated seeds; and
A method for enhancing the functional component content of sprout crops, comprising a third step of cultivating sprouts by treating the germinated seeds with chemical substances.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110064063A (en) * | 2009-12-07 | 2011-06-15 | 김재일 | Cutivation method of wheat sprowt or barley sprowt for rice wrapped in leaves and shredded vegetables and wheat sprowt or barley sprowt cultivated by such a method |
| KR20110115468A (en) * | 2010-04-15 | 2011-10-21 | 한국식품연구원 | Cultivation method of plant with increased contents of functional materials |
| KR101461859B1 (en) * | 2014-05-27 | 2014-11-12 | (주)라파프로폴리스 | Method for cultivating bean sprouts using non alcohol water soluble propolis and alkali-water |
| KR20150034024A (en) * | 2013-09-25 | 2015-04-02 | 대한민국(농촌진흥청장) | A method for preparing extracts of wheat sprouts, oat sprouts or rye sprouts having increased content of policosanol |
| KR101815616B1 (en) * | 2017-06-14 | 2018-01-05 | 정동흔 | A cultivation method of bean sprouts using onion extract |
| KR20200059488A (en) * | 2018-11-21 | 2020-05-29 | 주식회사 푸마시 | Method for cultivating barley sprout with increased saponarin content and barley sprout cultivated by the same |
-
2020
- 2020-12-16 KR KR1020200176750A patent/KR102606386B1/en active IP Right Grant
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110064063A (en) * | 2009-12-07 | 2011-06-15 | 김재일 | Cutivation method of wheat sprowt or barley sprowt for rice wrapped in leaves and shredded vegetables and wheat sprowt or barley sprowt cultivated by such a method |
| KR20110115468A (en) * | 2010-04-15 | 2011-10-21 | 한국식품연구원 | Cultivation method of plant with increased contents of functional materials |
| KR20150034024A (en) * | 2013-09-25 | 2015-04-02 | 대한민국(농촌진흥청장) | A method for preparing extracts of wheat sprouts, oat sprouts or rye sprouts having increased content of policosanol |
| KR101461859B1 (en) * | 2014-05-27 | 2014-11-12 | (주)라파프로폴리스 | Method for cultivating bean sprouts using non alcohol water soluble propolis and alkali-water |
| KR101815616B1 (en) * | 2017-06-14 | 2018-01-05 | 정동흔 | A cultivation method of bean sprouts using onion extract |
| KR20200059488A (en) * | 2018-11-21 | 2020-05-29 | 주식회사 푸마시 | Method for cultivating barley sprout with increased saponarin content and barley sprout cultivated by the same |
Non-Patent Citations (5)
| Title |
|---|
| Do Manh Cuong 등, "Effect of Salinity Stress on Phenylpropanoid Genes Expression and Related Gene Expression in Wheat Sprout", Agronomy, 10, 390(13 March 2020) * |
| J. Agric. Food Chem. Vol 53, pps 5583-5586(2005.06.07.)* * |
| Jean-Robert Ioset et al., "Flavonoid profiling among wild type and related GM wheat varieties"Plant Mol Biol. 65:645-654(2007) * |
| Plant Mol Biol. Vol 65:645-654(2007.09.12.)* * |
| SIBEL IRMAK et al., "Policosanol Contents and Compositions of Wheat Varieties", J. Agric. Food Chem. 53, 5583-5586(2005) * |
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