KR20220077364A - Extracting method of phenol compounds and flavonoid from oat sprouts - Google Patents
Extracting method of phenol compounds and flavonoid from oat sprouts Download PDFInfo
- Publication number
- KR20220077364A KR20220077364A KR1020200166217A KR20200166217A KR20220077364A KR 20220077364 A KR20220077364 A KR 20220077364A KR 1020200166217 A KR1020200166217 A KR 1020200166217A KR 20200166217 A KR20200166217 A KR 20200166217A KR 20220077364 A KR20220077364 A KR 20220077364A
- Authority
- KR
- South Korea
- Prior art keywords
- flavonoids
- phenolic compounds
- extract
- oats
- oat powder
- Prior art date
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B63/00—Purification; Separation; Stabilisation; Use of additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0288—Applications, solvents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/68—Purification; separation; Use of additives, e.g. for stabilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
본 발명은 귀리에 함유되어 있는 페놀화합물와 플라보노이드를 대량으로 추출시켜 주기 위한 방법에 관한 것으로, 동결건조된 귀리를 분쇄하여 분말로 형성한 후, 30~60분동안 1 ~ 3KGy로 방사선을 조사하고, 추출용매로서 65 ~ 75농도%의 메탄올을 사용하여 추출하는 것을 특징으로 한다.The present invention relates to a method for extracting a large amount of phenolic compounds and flavonoids contained in oats. After grinding freeze-dried oats to form a powder, irradiated with radiation at 1 to 3KGy for 30 to 60 minutes, It is characterized in that the extraction is performed using methanol at a concentration of 65 to 75% as an extraction solvent.
Description
본 발명은 귀리로부터 대량의 페놀화합물과 플라보노이드를 추출할 수 있도록 하는 추출방법에 관한 것이다.The present invention relates to an extraction method capable of extracting a large amount of phenolic compounds and flavonoids from oats.
폴리페놀 화합물(polyphenolic compound)은 식물 내에 가장 많이 존재하는 물질 중의 하나로 일반적으로 식물들의 2차 대사 작용에 의한 산물로 생성되며, 주로 당(glycoside)에 부착된 형태로 존재하나 경우에 따라 아글리콘(aglycone) 형태로도 존재한다. Polyphenolic compounds are one of the most abundant substances in plants, and are generally produced as a product of secondary metabolism of plants. It also exists in the form of aglycone).
최근 폴리페놀 화합물이 주목받고 있는 이유는 생체내에서 산화를 방지하는 기능, 즉, 항산화 기능뿐만 아니라 항돌연변이, 항암 및 항염증 활성 등을 가지고 있어 건강 유지와 질병 예방 등에 기여할 것으로 기대되기 때문이다. 또한, 폴리페놀 화합물은 콜레스테롤이 소화관으로 흡수되는 것을 막아주기 때문에 혈중 콜레스테롤의 수치를 낮게 해주는 기능도 한다.The reason why polyphenol compounds have recently been attracting attention is because they have anti-oxidation functions in vivo, that is, not only antioxidant functions, but also anti-mutation, anti-cancer and anti-inflammatory activities, and are expected to contribute to health maintenance and disease prevention. In addition, polyphenol compounds prevent cholesterol from being absorbed into the digestive tract, thereby lowering the level of cholesterol in the blood.
플라보노이드는 폴리페놀 화합물 중에서 가장 큰 그룹으로, 과일과 채소 등에서 담황색 또는 노란색을 띠는 색소 화합물이며, '비타민 P' 라고도 불리고 있다.Flavonoids are the largest group of polyphenol compounds, and are pigment compounds that have a pale yellow or yellow color in fruits and vegetables, and are also called 'vitamin P'.
플라보노이드는 신체 내에서 항바이러스, 항히스타민, 항산화, 모세혈관 투과 억제, 혈압 강하, 살충, 간세포 보호, 혈당 강하 등의 작용을 하며, 특이한 부작용이 없는 것으로 보고되었다. 또한, 암 발생의 위험을 줄이고 모든 질병의 원인이 되는 생체내 산화 작용을 억제한다는 사실이 알려지면서 플라보노이드 물질의 개발 및 활용에 관한 관심이 지속적으로 커져가고 있다. It has been reported that flavonoids have antiviral, antihistamine, antioxidant, capillary permeation inhibition, blood pressure lowering, insecticidal, hepatocellular protection, and blood sugar lowering actions in the body, and have no specific side effects. In addition, as it is known that it reduces the risk of cancer and inhibits oxidation in vivo, which is the cause of all diseases, interest in the development and utilization of flavonoids is continuously increasing.
위에서 언급한 다양한 효과를 이용하기 위해 폴리페놀 화합물이나 플라보노이드를 보리 등의 천연식물로부터 추출하는 시도가 꾸준히 이루어지고 있는 실정이다.In order to utilize the various effects mentioned above, attempts are being made to extract polyphenol compounds or flavonoids from natural plants such as barley.
종래, 천연식물로부터 폴리페놀 화합물과 플라보노이드를 추출함에 있어 단순히 용매를 이용하여 추출하는 방식임에 따라 천연식물로부터 추출되는 페놀화합물과 플라보노이드의 함량이 많지 않았다.Conventionally, in extracting polyphenol compounds and flavonoids from natural plants, the content of phenolic compounds and flavonoids extracted from natural plants was not large as the extraction method was simply using a solvent.
또한, 천연식물 중에서도 귀리에 함유되어 있는 폴리페놀 화합물과 플라보노이드를 추출하는 기술에 대해서는 아직까지 알려진 바가 없었다.In addition, technology for extracting polyphenol compounds and flavonoids contained in oats among natural plants has not yet been known.
본 발명자는 귀리에 함유되어 있는 페놀화합물과 플라보노이드의 함량을 측정하여 추출하는 방법을 예의 연구한 결과, 동결건조한 귀리분말에 방사선의 조사유무와 추출용매로 사용되는 메탄올의 농도에 따라 추출되는 페놀화합물과 플라보노이드의 함량이 변화되는 것을 확인하고 본 발명을 완성하였다.As a result of diligent research on the extraction method by measuring the content of phenolic compounds and flavonoids contained in oats, the present inventors found that phenolic compounds extracted according to the presence or absence of radiation and the concentration of methanol used as an extraction solvent in the freeze-dried oat powder. It was confirmed that the content of and flavonoids was changed, and the present invention was completed.
따라서, 본 발명은 최적의 방사선량으로 조사함은 물론 최적 농도의 메탄올을 투입하여 추출하도록 함으로써 페놀화합물와 플라보노이드를 최대량으로 추출할 수 있도록 하는 귀리에 함유된 페놀화합물과 플라보노이드의 추출방법을 제공하는 데 그 목적이 있다.Accordingly, the present invention provides a method for extracting phenolic compounds and flavonoids contained in oats, which allows extraction of phenolic compounds and flavonoids in the maximum amount by not only irradiating with an optimal radiation dose but also extracting by adding methanol of an optimal concentration. There is a purpose.
상기의 과제를 해결하기 위한 해결수단은, 동결건조된 귀리를 분쇄하여 분말로 형성한 후, 30~60분동안 1 ~ 3KGy로 방사선을 조사하고, 추출용매로서 65 ~ 75농도%의 메탄올을 사용하여 추출하는 것을 특징으로 한다.The solution to the above problem is to grind lyophilized oats to form a powder, then irradiate with 1 to 3KGy for 30 to 60 minutes, and use 65 to 75 concentration% of methanol as an extraction solvent. It is characterized in that it is extracted.
여기서, 최대치의 페놀화합물과 플라보노이드를 추출하기 위해, 각기 다른 방사선량으로 조사된 귀리분말의 추출액에 함유된 페놀화합물과 플라보노이드의 함량을 확인하여 서로 비교하는 함량 확인단계와, 상기 단계를 거쳐 가장 많은 량이 함유된 추출액을 선별하고, 해당 추출액의 귀리분말에 조사된 방사선량을 확인하는 방사선량 확인단계를 거쳐 최적의 방사선량을 결정하는 것이 바람직하다.Here, in order to extract the maximum amount of phenolic compounds and flavonoids, the contents of phenolic compounds and flavonoids contained in the extract of oat powder irradiated with different radiation doses are checked and compared with each other, and the content confirmation step, It is desirable to determine the optimal radiation dose by selecting the extract containing the amount and going through the radiation dose checking step of checking the radiation dose irradiated to the oat powder of the extract.
이때, 상기 함량 확인단계에서, 페놀화합물은 추출액을 원심분리하여 취한 상등액 0.5 ~ 1.5㎖에 플론시약(Folin-ciocalteau reagent) 0.1㎖를 혼합한 후, 탄산나트륨(Na2CO3) 0.2㎖와 증류수 2㎖를 혼합하여 실온에서 1시간 방치한 다음 원심분리하여 취한 상등액의 흡광도를 측정하여 확인하고, 플라보노이드는 추출액을 원심분리하여 취한 상등액 0.5 ~ 1.5㎖에 90~98% 에탄올 1~2㎖를 첨가한 후, 5~15% 질화알루미늄(aluminium nitrate) 0.1㎖과 1M 아세트산칼륨(potassium acetate) 0.1㎖와 증류수 2.8㎖를 혼합하여 상온에서 20~40분간 방치한 다음 흡광도를 측정하여 확인하게 된다.At this time, in the content confirmation step, the phenolic compound is obtained by mixing 0.1 ml of Folin-ciocalteau reagent with 0.5 to 1.5 ml of the supernatant obtained by centrifuging the extract, and then 0.2 ml of sodium carbonate (Na 2 CO 3 ) and 2 distilled water ㎖ was mixed and left at room temperature for 1 hour, and then centrifuged to measure the absorbance of the supernatant to check, and flavonoids were obtained by centrifuging the extract and adding 1-2 mL of 90-98% ethanol to 0.5 to 1.5 mL of the supernatant. After that, 0.1 ml of 5-15% aluminum nitrate, 0.1 ml of 1M potassium acetate, and 2.8 ml of distilled water were mixed, left at room temperature for 20 to 40 minutes, and then the absorbance was measured to confirm.
본 발명의 귀리로부터 페놀화합물과 플라보노이드의 추출방법은, 귀리분말에 조사되는 방사선량을 변경시켜 주면서 추출되는 페놀화합물과 플라보노이드의 함량을 비교하여 확인함으로써 최적의 방사선량을 결정하여 추출물의 수득율 향상을 도모할 수 있는 효과가 있다.The extraction method of phenolic compounds and flavonoids from oats of the present invention determines the optimal radiation dose by comparing and confirming the contents of extracted phenolic compounds and flavonoids while changing the radiation dose irradiated to oat powder to improve the yield of the extract. There is an effect that can be achieved.
도 1은 본 발명에 따른 방사선 조사유무 및 방사선량에 따른 귀리에 함유되어 있는 페놀화합물의 함량을 보인 그래프,
도 2는 본 발명에 따른 방사선 조사유무 및 방사선량에 따른 귀리에 함유되어 있는 플라보노이드의 함량을 보인 그래프,
도 3은 본 발명에 따른 방사선 조사유무 및 방사선량에 따른 귀리에 함유되어 있는 항산화물질의 활성량을 보인 그래프.
도 4는 본 발명에 따른 메탄올의 농도에 따른 새싹귀리분말에 함유된 페놀화합물의 추출량 그래프,
도 5는 본 발명에 따른 메탄올의 농도에 따른 새싹귀리분말에 함유된 플라보노이드의 추출량 그래프.1 is a graph showing the content of phenolic compounds contained in oats according to the presence or absence of radiation and the amount of radiation according to the present invention;
2 is a graph showing the content of flavonoids contained in oats according to the presence or absence of radiation and the amount of radiation according to the present invention;
3 is a graph showing the active amount of antioxidants contained in oats according to the presence or absence of radiation and the amount of radiation according to the present invention.
4 is a graph of the extraction amount of phenolic compounds contained in sprout oat powder according to the concentration of methanol according to the present invention;
5 is a graph of the extraction amount of flavonoids contained in sprout oat powder according to the concentration of methanol according to the present invention.
이하, 본 발명의 바람직한 실시예에 따른 귀리로부터 대량의 페놀화합물과 플라보노이드의 추출방법을 상세히 설명하기로 한다.Hereinafter, a method for extracting a large amount of phenolic compounds and flavonoids from oats according to a preferred embodiment of the present invention will be described in detail.
도시된 바와 같이, 본 발명에 따른 귀리로부터 대량의 페놀화합물과 플라보노이드의 추출방법은 동결건조된 귀리분말에 방사선을 조사함으로써 페놀화합물과 플라보노이드를 대량으로 추출할 수 있게 된다.As shown, in the method for extracting a large amount of phenolic compounds and flavonoids from oats according to the present invention, phenolic compounds and flavonoids can be extracted in large quantities by irradiating the freeze-dried oat powder with radiation.
구체적으로, 귀리를 동결전조하거나 시중에 동결건조된 귀리를 구매하여 분쇄하여 분말화한다. 물론 동결건조된 귀리분말을 구매하여 사용할 수도 있다.Specifically, freeze-form oats or purchase freeze-dried oats in the market and pulverize them. Of course, you can purchase and use freeze-dried oat powder.
귀리분말에 방사선을 조사하는데, 동일한 량으로 조사하는 것이 아닌 1 ~ 3KGy로 대략 30~60분동안 방사선을 조사한다.The oat powder is irradiated with radiation, not the same amount, but irradiated with 1 ~ 3KGy for about 30 to 60 minutes.
방사선량이 1KGy 미만이면 조사되량이 너무 적어 효과가 미미하고, 방사선량이 3KGy를 초과하면 너무 과다하게 조사되어 귀리분말이 방사능을 갖게 될 위험이 있다.If the radiation dose is less than 1KGy, the effect is insignificant because the radiation dose is too small.
귀리분말에 방사선을 조사한 후에는 추출용매로서 메탄올을 사용하여 추출하게 되는데, 추출시에는 10kHz 내지 50kHz에서 10W 내지 300W의 세기로 100 ~140분 동안 초음파 처리과정을 거쳐 추출한다.After irradiating the oat powder with radiation, it is extracted using methanol as an extraction solvent. During extraction, the oat powder is extracted through a sonication process for 100 to 140 minutes at an intensity of 10W to 300W at 10kHz to 50kHz.
초음파 처리를 통해, 귀리분말의 효과적인 세포벽 파괴로 본 발명에서 추출하고자 하는 활성물질인 페놀화합물과 플라보노이드의 용출을 보다 더 증진시켜 줄 수 있다.Through sonication, it is possible to further enhance the dissolution of phenolic compounds and flavonoids, which are the active substances to be extracted in the present invention, by effectively destroying the cell wall of the oat powder.
메탄올을 이용한 추출방법은 초음파 처리 후 원심분리기(Beckman, USA, CA)를 이용하여 3 ~ 5℃의 온도에서 1000~1200RPM으로 7 ~ 15분 동안 각각 원심분리한 다음 상등액을 취하고, 이렇게 취한 상등액을 기준 시료용액으로 사용하게 된다.The extraction method using methanol is centrifuged at a temperature of 3 to 5° C. at 1000 to 1200 RPM for 7 to 15 minutes using a centrifugal separator (Beckman, USA, CA) after ultrasonic treatment, and then the supernatant is taken, and the supernatant thus obtained is separated. It will be used as a reference sample solution.
이때, 추출용매로 사용되는 상기 메탄올은 농도에 따라 추출액에 함유된 페놀화합물과 플라보노이드의 함량에 차이가 발생하게 된다.At this time, the methanol used as an extraction solvent causes a difference in the contents of phenolic compounds and flavonoids contained in the extract according to the concentration.
메탄올의 농도에 따라 귀리분말(새싹귀리 분말)의 추출물에 함유된 페놀화합물의 함량과 플라보노이드의 함량은 각각 도 4와 도 5에 도시되어 있다. 도 4 및 도 5의 페놀화합물의 함량과 플라보노이드의 함량 수치는 g중의 mg gallic acid equivalents(GAE)로 나타내었다.The content of phenolic compounds and the content of flavonoids contained in the extract of oat powder (sprout oat powder) according to the concentration of methanol are shown in FIGS. 4 and 5, respectively. The phenolic compound content and flavonoid content values of FIGS. 4 and 5 are expressed as mg gallic acid equivalents (GAE) in g.
도 4와 도 5를 보면 귀리분말에 함유되어 있는 페놀화합물과 플라보노이드의 추출량은 메탄올의 농도가 진할수록 추출되는 양이 증가되는 것으로 나타났다. 65농도% 이하의 농도를 갖는 메탄올은 페놀화합물과 플라보노이드의 추출량이 많지 않고, 75농도% 이상의 농도를 갖는 메탄올은 오히려 추출량이 적으며, 비용이 증가됨에 따라 경제적이지 못하다.4 and 5, it was found that the extraction amount of phenolic compounds and flavonoids contained in oat powder increased as the concentration of methanol increased. Methanol having a concentration of 65% or less does not extract much phenolic compounds and flavonoids, and methanol having a concentration of 75% or more has a small extraction amount and is not economical as the cost increases.
따라서, 본 발명에 사용되는 메탄올은 65 ~ 75농도%의 메탄올을 사용하여 페놀화합물과 플라보노이드를 추출하도록 함이 바람직하다.Therefore, the methanol used in the present invention is preferably used to extract phenolic compounds and flavonoids using methanol at a concentration of 65 to 75%.
한편, 귀리분말로부터 최대치의 페놀화합물과 플라보노이드를 추출하기 위해서, 최적의 방사선량으로 조사하도록 함이 바람직하다.On the other hand, in order to extract the maximum amount of phenolic compounds and flavonoids from the oat powder, it is desirable to irradiate with an optimal radiation dose.
최적의 방사선량을 결정하기 위해, 함량 확인단계와 방사선량 확인단계를 거치게 된다.In order to determine the optimal radiation dose, it goes through a content check step and a radiation dose check step.
함량 확인단계에서는 각기 다른 량으로 조사된 귀리분말 추출액을 후술되는 방법으로 이용하여 페놀화합물과 플라보노이드의 함량을 확인하고, 서로 비교하게 된다.In the content confirmation step, the contents of phenolic compounds and flavonoids are checked and compared with each other by using the oat powder extract irradiated in different amounts by the method described below.
더 구체적으로 페놀화합물의 함량은 추출액을 원심분리하여 취한 상등액 0.5 ~ 1.5㎖에 플론시약(Folin-ciocalteau reagent) 0.1㎖를 혼합하여 실온에 약 2시간 정도 방치한 후, 탄산나트륨(Na2CO3) 0.2㎖와 증류수 2㎖를 혼합하여 실온 암조건에서 1시간 방치한 다음 원심분리하여 상등액을 취하고, 750nm에서 분광광도계(UV spectrophotometer)로 흡광도 측정을 통해 정량하여 확인하게 된다.More specifically, the content of the phenolic compound is determined by mixing 0.1 ml of Folin-ciocalteau reagent with 0.5 to 1.5 ml of the supernatant obtained by centrifuging the extract, leaving it at room temperature for about 2 hours, and then sodium carbonate (Na 2 CO 3 ) 0.2 ml and 2 ml of distilled water are mixed, left for 1 hour in the dark at room temperature, centrifuged to take the supernatant, and the absorbance is measured at 750 nm with a UV spectrophotometer to quantify and confirm.
정량을 위한 검량선은 갈산(Gallic acid (Sigma G7384)) 1mg을 70% 메탄올 1㎖에 용해시켜 2, 4, 6, 8, 10 ug/㎖가 되도록 조제한 후 상기 상등액과 같은 방법으로 처리하여 750 nm에서 흡광도를 측정하여 작성하였다.The calibration curve for quantification is 2, 4, 6, 8, 10 ug/ml by dissolving 1 mg of gallic acid (Sigma G7384) in 1 ml of 70% methanol, and then treating the supernatant in the same manner as above to 750 nm was prepared by measuring the absorbance.
도 1에는 동결건조한 귀리분말과 1KGy와 3KGy로 방사선을 조사한 귀리분말에서 추출한 추출액에 함유된 총 페놀화합물의 함량 그래프가 도시되어 있다.1 is a graph showing the content of total phenolic compounds contained in extracts extracted from freeze-dried oat powder and oat powder irradiated with 1 KGy and 3 KGy radiation.
그래프에 도시된 바와 같이, 방사선을 조사하지 않은 귀리분말에 비해 방사선을 조사한 귀리분말에서 추출한 페놀화합물의 함량이 많았으며, 방사선을 조사한 귀리분말중에서도 1KGy보다는 3KGy로 조사한 귀리분말의 페놀화합물 함량이 증가하였음을 확인할 수 있다.As shown in the graph, the phenolic compound content extracted from the irradiated oat powder was higher than that of the non-irradiated oat powder, and the phenolic compound content of the irradiated oat powder increased with 3KGy rather than 1KGy among the irradiated oat powder. It can be confirmed that
그리고 방사선량 확인단계는 함량 확인단계를 거쳐 가장 많은 량이 함유된 추출액을 선별하고, 해당 추출액의 귀리분말에 조사된 방사선량을 확인하게 되고, 이를 통해 최적의 방사선량을 결정하게 되는 것이다.In the radiation dose confirmation step, the extract containing the largest amount is selected through the content check step, and the radiation dose irradiated to the oat powder of the extract is checked, and through this, the optimal radiation dose is determined.
구체적으로, 플라보노이드는 추출액을 원심분리하여 취한 상등액 0.5 ~ 1.5㎖에 90~98% 에탄올 1~2㎖를 첨가한 후, 5~15% 질화알루미늄(aluminium nitrate) 0.1㎖과 1M 아세트산칼륨(potassium acetate) 0.1㎖와 증류수 2.8㎖를 혼합하여 상온에서 20~40분간 방치한 다음 415nm에서 분광광도계(UV spectrophotometer)로 흡광도 측정을 통해 정량하여 확인하게 된다.Specifically, flavonoids are obtained by adding 1 to 2 ml of 90 to 98% ethanol to 0.5 to 1.5 ml of a supernatant obtained by centrifugation of the extract, and then 0.1 ml of 5 to 15% aluminum nitrate and 1M potassium acetate. ) Mix 0.1 ml and 2.8 ml of distilled water, leave at room temperature for 20 to 40 minutes, and then quantify and confirm by measuring absorbance at 415 nm with a UV spectrophotometer.
정량을 위한 검량선은 루틴(Rutin (PHL 89270)) 1mg을 70% 메탄올 1㎖에 용해시켜 5, 20, 40, 80 ug/㎖가 되도록 조제한 후, 상기 상등액과 같은 방법으로 처리하여 415 nm에서 흡광도를 측정하여 작성하였다.The calibration curve for quantitation was prepared by dissolving 1 mg of Rutin (PHL 89270) in 1 ml of 70% methanol to make 5, 20, 40, 80 ug/ml, and then treating the supernatant in the same way as the absorbance at 415 nm. was measured and written.
도 2에는 동결건조한 귀리분말과 1KGy와 3KGy로 방사선을 조사한 귀리분말에서 추출한 추출액에 함유된 총 플라보노이드의 함량 그래프가 도시되어 있다.2 is a graph showing the content of total flavonoids contained in lyophilized oat powder and extracts extracted from oat powder irradiated with 1KGy and 3KGy.
그래프에 도시된 바와 같이, 방사선을 조사하지 않은 귀리분말에 비해 방사선을 조사한 귀리분말에서 추출한 플라보노이드의 함량이 많았으며, 방사선을 조사한 귀리분말중에서도 1KGy보다는 3KGy로 조사한 귀리분말의 플라보노이드의 함량이 증가하였음을 확인할 수 있다.As shown in the graph, the content of flavonoids extracted from the irradiated oat powder was higher than that of the non-irradiated oat powder, and among the irradiated oat powder, the content of flavonoids in the oat powder irradiated with 3KGy rather than 1KGy was increased. can confirm.
한편, 본 발명에서는 동결전조한 귀리분말과 방사선을 조사한 귀리분말에 함유된 항산화물질의 활성량을 측정하였으며, 그 결과가 도 3에 도시되어 있다.Meanwhile, in the present invention, the active amount of antioxidants contained in the oat powder irradiated with the oat powder irradiated with the freeze-formed oat powder was measured, and the results are shown in FIG. 3 .
실시예로서, 동결건조한 귀리분말과 1KGy, 3KGy로 방사선 조사한 귀리분말 2g에 추출용매로서 70% 메탄올 10㎖를 투입하여 25℃에서 24시간 추출한 후 30분 동안 8,000 ×g에서 원심분리기(Beckman, USA, CA)를 이용하여 고형분을 제거하였다. 원심분리된 상등액만을 모아 0.45um syringe filter하여 실험에 사용하였다. 이 중 50㎕에 0.1M Tris-HCL (pH 7.4) 450㎕을 가한 후 0.3mM DPPH (α,α‘β-picrylhydrazyl) solution in Methanol을 500㎕ 가하여 혼합(voltex mixer 혼합) 한 뒤 20분간 암조건에서 반응시킨 후에 517nm에서 분광광도계(UV spectrophotpmeter)로 흡광도를 측정한 후 다음 식에 의해 계산하여 백분율로 나타내었으며, 갈산(Gallic acid (Sigma G7384))를 표준물질로 이용하였다.As an example, 10 ml of 70% methanol as an extraction solvent was added to freeze-dried oat powder and 2 g of oat powder irradiated with 1 KGy and 3 KGy, extracted at 25° C. for 24 hours, and then centrifuged at 8,000 × g for 30 minutes (Beckman, USA). , CA) was used to remove solids. Only the centrifuged supernatant was collected, and a 0.45um syringe filter was used for the experiment. Of these, 450 μl of 0.1M Tris-HCL (pH 7.4) was added to 50 μl, and 500 μl of 0.3 mM DPPH (α,α'β-picrylhydrazyl) solution in methanol was added and mixed (voltex mixer mixing), followed by dark conditions for 20 minutes. After reacting at 517 nm, absorbance was measured with a spectrophotometer (UV spectrophotopmeter) and expressed as a percentage by calculation by the following equation, and gallic acid (Sigma G7384) was used as a standard material.
그래프에 도시된 바와 같이, 방사선을 조사하지 않은 귀리분말에 비해 방사선을 조사한 귀리분말에서 추출한 항산화물질의 활성량이 높고, 방사선을 조사한 귀리분말중에서도 1KGy보다는 3KGy로 조사한 귀리분말의 항산화물질의 활성량이 더 증가하였음을 확인할 수 있다.As shown in the graph, the active amount of antioxidants extracted from irradiated oat powder was higher than that of oat powder not irradiated with radiation, and the antioxidant activity of oat powder irradiated with 3KGy rather than 1KGy among oat powder irradiated with radiation was higher. It can be seen that increased
없음doesn't exist
Claims (4)
A large amount of phenolic compounds from oats, characterized in that after grinding the freeze-dried oats to form a powder, irradiating radiation at 1 to 3KGy for 30 to 60 minutes, and using methanol at a concentration of 65 to 75% as an extraction solvent and flavonoid extraction method.
최대치의 페놀화합물과 플라보노이드를 추출하기 위해,
각기 다른 방사선량으로 조사된 귀리분말의 추출액에 함유된 페놀화합물과 플라보노이드의 함량을 확인하여 서로 비교하는 함량 확인단계와,
상기 단계를 거쳐 가장 많은 량이 함유된 추출액을 선별하고, 해당 추출액의 귀리분말에 조사된 방사선량을 확인하는 방사선량 확인단계를 거쳐 최적의 방사선량을 결정하는 것을 특징으로 하는 귀리로부터 대량의 페놀화합물과 플라보노이드 추출방법.
According to claim 1,
To extract the maximum amount of phenolic compounds and flavonoids,
A content confirmation step of comparing the contents of phenolic compounds and flavonoids contained in the extract of oat powder irradiated with different radiation doses;
A large amount of phenolic compounds from oats, characterized in that the extract containing the largest amount is selected through the above step, and the optimal radiation dose is determined through the radiation dose checking step of confirming the radiation dose irradiated to the oat powder of the extract and flavonoid extraction method.
상기 함량 확인단계에서, 페놀화합물은 추출액을 원심분리하여 취한 상등액 0.5 ~ 1.5㎖에 플론시약(Folin-ciocalteau reagent) 0.1㎖를 혼합한 후, 탄산나트륨(Na2CO3) 0.2㎖와 증류수 2㎖를 혼합하여 실온에서 1시간 방치한 다음 원심분리하여 취한 상등액의 흡광도를 측정하여 확인하는 것을 특징으로 하는 귀리로부터 대량의 페놀화합물과 플라보노이드의 추출방법.
3. The method of claim 2,
In the content confirmation step, the phenolic compound is obtained by mixing 0.1 ml of Folin-ciocalteau reagent with 0.5 to 1.5 ml of the supernatant obtained by centrifuging the extract, and then 0.2 ml of sodium carbonate (Na 2 CO 3 ) and 2 ml of distilled water. A method of extracting a large amount of phenolic compounds and flavonoids from oats, characterized by measuring and confirming the absorbance of the supernatant obtained by centrifugation after mixing and leaving at room temperature for 1 hour.
상기 함량 확인단계에서, 플라보노이드는 추출액을 원심분리하여 취한 상등액 0.5 ~ 1.5㎖에 90~98% 에탄올 1~2㎖를 첨가한 후, 5~15% 질화알루미늄(aluminium nitrate) 0.1㎖과 1M 아세트산칼륨(potassium acetate) 0.1㎖와 증류수 2.8㎖를 혼합하여 상온에서 20~40분간 방치한 다음 흡광도를 측정하여 확인하는 것을 특징으로 하는 귀리로부터 대량의 페놀화합물과 플라보노이드의 추출방법.3. The method of claim 2,
In the content confirmation step, flavonoids are obtained by centrifuging the extract and adding 1 to 2 ml of 90 to 98% ethanol to 0.5 to 1.5 ml of the supernatant, then 5 to 15% aluminum nitrate 0.1 ml and 1M potassium acetate A method of extracting a large amount of phenolic compounds and flavonoids from oats, characterized in that 0.1 ml of (potassium acetate) and 2.8 ml of distilled water are mixed, left at room temperature for 20 to 40 minutes, and then the absorbance is measured and confirmed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020200166217A KR102563978B1 (en) | 2020-12-02 | 2020-12-02 | Extracting method of phenol compounds and flavonoid from oat sprouts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020200166217A KR102563978B1 (en) | 2020-12-02 | 2020-12-02 | Extracting method of phenol compounds and flavonoid from oat sprouts |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20220077364A true KR20220077364A (en) | 2022-06-09 |
| KR102563978B1 KR102563978B1 (en) | 2023-08-03 |
Family
ID=81986017
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020200166217A KR102563978B1 (en) | 2020-12-02 | 2020-12-02 | Extracting method of phenol compounds and flavonoid from oat sprouts |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR102563978B1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115490662A (en) * | 2022-09-16 | 2022-12-20 | 云南省农业科学院生物技术与种质资源研究所 | Method for extracting flavonoid compounds from oryza verrucosa |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110086138A (en) * | 2008-11-14 | 2011-07-27 | 삐에르화브르데르모-코스메띠끄 | Extract of aerial parts of oats harvested before ear emergence |
| KR20110085758A (en) | 2010-01-21 | 2011-07-27 | 대한민국(관리부서:농촌진흥청장) | Extracts from young barley leaves including polyphenol compounds and their manufacturing method |
| KR20140006274A (en) | 2012-07-02 | 2014-01-16 | 전남대학교산학협력단 | Method for optimal extraction of resveratrol from peanut sprout |
| CN107456429A (en) * | 2017-08-30 | 2017-12-12 | 上海家化联合股份有限公司 | A kind of oat rudiment extract and its application in cosmetics |
-
2020
- 2020-12-02 KR KR1020200166217A patent/KR102563978B1/en active IP Right Grant
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110086138A (en) * | 2008-11-14 | 2011-07-27 | 삐에르화브르데르모-코스메띠끄 | Extract of aerial parts of oats harvested before ear emergence |
| KR20110085758A (en) | 2010-01-21 | 2011-07-27 | 대한민국(관리부서:농촌진흥청장) | Extracts from young barley leaves including polyphenol compounds and their manufacturing method |
| KR20140006274A (en) | 2012-07-02 | 2014-01-16 | 전남대학교산학협력단 | Method for optimal extraction of resveratrol from peanut sprout |
| CN107456429A (en) * | 2017-08-30 | 2017-12-12 | 上海家化联合股份有限公司 | A kind of oat rudiment extract and its application in cosmetics |
Non-Patent Citations (2)
| Title |
|---|
| J. Korean Soc. Food Sci. Nutr., 2002, 31, 2, 246-250, 1부.* * |
| J. Korean Soc. Food Sci. Nutr., 2016, 45, 11, 1681-1684, 1부.* * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115490662A (en) * | 2022-09-16 | 2022-12-20 | 云南省农业科学院生物技术与种质资源研究所 | Method for extracting flavonoid compounds from oryza verrucosa |
| CN115490662B (en) * | 2022-09-16 | 2023-06-23 | 云南省农业科学院生物技术与种质资源研究所 | Method for extracting flavonoid compounds from wild rice in verruca vulgaris |
Also Published As
| Publication number | Publication date |
|---|---|
| KR102563978B1 (en) | 2023-08-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Najafabad et al. | Free radical scavenging capacity and antioxidant activity of methanolic and ethanolic extracts of plum (Prunus domestica L.) in both fresh and dried samples | |
| CN106573024A (en) | Composition for improving liver function, containing extract of dendropanax morbifera | |
| Pandey et al. | Antioxidant activity of ethanolic extract of Annona squamosa Linn Bark | |
| Babotă et al. | Chemical composition, diuretic, and antityrosinase activity of traditionally used Romanian Cerasorum stipites | |
| KR102563978B1 (en) | Extracting method of phenol compounds and flavonoid from oat sprouts | |
| Chua et al. | Water soluble phenolics, flavonoids and anthocyanins extracted from jaboticaba berries using maceration with ultrasonic pretreatment | |
| Ghanem et al. | The effect of herbal tea from Balanites aegyptiaca fruits on streptozotocin-induced diabetes mellitus in rats | |
| Moussaid et al. | In vivo anti-inflammatory and in vitro antioxidant activities of Moroccan medicinal plants | |
| Cahyaningrum et al. | Antioxidant activity of loloh Malaka fruit (Phyllanthus emblica L.) in ayurveda medication: how it supports environmental conservation | |
| Leite et al. | Chemical Profile and Evaluation of the Antioxidant and Anti-Acetylcholinesterase Activities of Annona squamosa L.(Annonaceae) Extracts. Foods 10, 2343 | |
| Ramdan et al. | Anti-glycation and radical scavenging activities of hydro-alcohol and aqueous extracts of nine species from Lamiaceae family | |
| Mahajan et al. | Evaluation of Punica granatum fruit peels extracts for its free radical scavenging and anti-inflammatory activity | |
| Maidadi et al. | Efficacy of Rytigynia senegalensis Blume on Free Radical Scavenging, Inhibition of α‐Amylase and α‐Glucosidase Activity, and Blood Glucose Level | |
| Wang et al. | Antioxidative effect of large molecular polymeric pigments extracted from zijuan pu-erh tea in vitro and in vivo | |
| Saad et al. | MULTIVARIATE ANALYSIS OF POLYPHENOLIC CONTENT AND IN VITRO ANTIOXIDANT CAPACITIES OF DIFFERENT FRACTIONS OF LEPIDIUM SATIVUM L. AERIAL PARTS AND ROOTS FROM ALGERIA. | |
| Kumar et al. | Potential role of Tribulus terrestris fruit extract in inhibition of advanced glycation end products | |
| Carole et al. | Comparison of phytochemical, iron chelating, and free radical scavenging activity of fresh Ribes nigrum (Black Currant) and nutraceutical C24/7 | |
| KR20240061173A (en) | Extracting method of phenol compounds and flavonoid from kudzu vine using gamma irradiation | |
| Julianti et al. | Phytochemical, Antioxidant Analysis and In Vitro Xanthine Oxidase Inhibitory Activity of Kaempferia parviflora and Kaempferia galanga. | |
| AU2021104456A4 (en) | Polyherbal ayurvedic formulation as a potential anti-oxidant agent. | |
| Baghalpour et al. | Antinociceptive, anti-inflammatory, and antioxidant studies on Withania somnifera (L.) Dunal | |
| Guettaf et al. | Contribution to Pharmacological Valorisation of Algerian Arctium minus (Hill) Bernh. Subsp. Atlanticum (Pomel) Maire; Antioxidant an d Acetylcholinesterase Inhibitory Activities | |
| Musewu et al. | In vitro evaluation of fruit extracts of Annona stenophylla diels and Flacourtia indica for incorporation into formulations for management of cancer | |
| Srinivasa et al. | Microwave assisted extraction and evaluation of in vitro antioxidant activity of Cinnamomum aromaticum | |
| Widyawaty et al. | Antioxidant Activity, Total Phenol, and Total Flavonoid of Syzygium Polyanthum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| E902 | Notification of reason for refusal | ||
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant |