KR20220074093A - Manufacturing method of feed additive for companion animal - Google Patents

Abstract

The method for producing a companion animal feed additive of the present invention comprises the step of proteolytically decomposing a mixture of chicken liver and pig liver to a slaughtering by-product with a proteolytic enzyme to obtain a protein degradation product.

Description

Companion animal feed additive manufacturing method {Manufacturing method of feed additive for companion animal}

The present invention relates to a method for manufacturing a companion animal feed additive. More specifically, the present invention relates to a method for producing a companion animal feed additive comprising the step of proteolytically decomposing a mixture of chicken liver and pig liver added to slaughtering by-products with a proteolytic enzyme to obtain a protein degradation product.

In Korea, more than 10 million people are raising companion animals, and thus, consumers' interest in products related to companion animals is increasing.

Feed is the most essential for raising companion animals among companion animal-related products, and it should be able to provide nutrition for balanced growth and activity to companion animals. Currently, various feeds that can provide sufficient and balanced nutrition to companion animals are being developed and marketed, but there are many problems that companion animals do not like, so a method to improve this is needed.

In addition, the cost of feed is gradually increasing to the extent that there is a report (National Council for Green Consumers and Nature) that dog owners spend an average of 140,000 won per month for feed and snacks. It is possible and there is a need for a method to increase palatability.

In this regard, inedible slaughtering by-products consisting of intestines, fat, head, and feet are decomposed with animal proteolytic enzymes, and proteolytic substances obtained by adding phosphoric acid at the stage of completion of the decomposition reaction are converted into corn, soybean meal, meat meal, animal fat and salt. Although the technology of adding to the configured feed (Korean Patent No. 10-0394581) has been developed, it is necessary to develop a method to increase palatability.

Korean Patent No. 10-0394581

An object of the present invention is to provide a method for manufacturing a feed additive that can make feed appeal to companion animals with higher preference.

Another object of the present invention is to provide a feed additive that is manufactured by the above method and can exhibit a synergistic effect of high feed preference.

Another object of the present invention is to provide a feed that can appeal to companion animals with a higher degree of preference by adding the feed additive.

According to one aspect of the present invention, there is provided a method for producing a companion animal feed additive comprising the step of proteolytically decomposing a mixture of chicken liver and pig liver added to slaughtering by-products with a proteolytic enzyme to obtain a protein degradation product.

In the method for producing a companion animal feed additive of the present invention, the step of obtaining the protein degradation product is a mixture of 200 to 400 parts by weight of chicken liver and 200 to 400 parts by weight of pig liver added to 400 parts by weight of slaughtering by-products by weight of Alcalase and Ara Preferably, the step is to obtain a protein degradation product by protein degradation with a zyme at 55 to 65° C. for 3 to 10 hours.

According to another aspect of the present invention, the present invention provides a companion animal feed additive manufactured by the method for manufacturing the companion animal feed additive.

According to another aspect of the present invention, the present invention provides a feed to which the companion animal feed additive is added.

According to the present invention, it is possible to manufacture a feed additive capable of greatly increasing the preference for feed for companion animals, particularly dogs, at low cost. In particular, the feed additive of the present invention can exhibit a more improved palatability enhancing effect compared to the slaughter by-product protein decomposition product developed for the purpose of enhancing the palatability of the existing feed composed of corn, soybean meal, meat meal, etc., so that it is easier to provide sufficient nutrition to companion animals. can provide

1 is a block diagram showing a method for manufacturing a feed additive according to an embodiment of the present invention.

The feed additive manufacturing method of the present invention was developed for the improved effect of the existing technology for increasing the palatability of feed, in particular, the method of Korean Patent No. 10-0394581. It characterized in that it comprises the step of obtaining a protein degradation product by digesting the protein with a degrading enzyme.

In the present invention, the slaughtering by-product means a by-product remaining after slaughtering a chicken and taking an edible part for food (used for human consumption). In general, as such by-products, intestines, fat, head, and feet are left, but chicken liver is generally not included here because it is used for food. Preferably, the slaughtering by-products are intestines and fat.

In the present invention, the step of obtaining the protein degradation product may be carried out using an apparatus or apparatus commonly used for enzymatic digestion of animal raw materials, for example, a reactor. Preferably, a reaction tank equipped with a temperature control device is used to provide a temperature suitable for enzyme activity. For example, proteolysis can be induced by adding the slaughtering by-products, chicken liver and pig liver to a reaction tank, adding a proteolytic enzyme thereto, and then incubating at a constant temperature for a certain period of time.

In the present invention, the mixture, that is, the mixture in which chicken liver and pig liver are added to the slaughtering by-product, is preferably in the form of the slaughtering by-product, chicken liver and pork liver shreds.

In this case, 200 to 400 parts by weight of chicken liver and 200 to 400 parts by weight of pig liver are preferably used based on 400 parts by weight of the slaughtering by-product. More preferably, based on 400 parts by weight of the slaughtering by-product, 250 to 350 parts by weight of chicken liver and 250 to 350 parts by weight of pig liver, more preferably 270 to 330 parts by weight of chicken liver and 270 to 330 parts by weight of pig liver, more preferably 290 to 310 parts by weight of chicken liver and 290 to 310 parts by weight of pig liver are used.

In the present invention, the mixture, that is, the mixture in which chicken liver and pig liver are added to the slaughtering by-product, may further contain other additives other than the slaughtering by-product, chicken liver and pig liver, for example, an additive for accelerating the enzymatic reaction. However, for the effect of the present invention, other additives are preferably included in less than 20% by weight, more preferably less than 10% by weight, more preferably less than 5% by weight, and more preferably other additives are not included. That is, the ratio of slaughtering by-products, chicken liver and pork liver is 100%.

Preferably, alcalase and arazyme are used together as the proteolytic enzyme in the present invention. Alcalase is a serine endopeptidase enzyme product containing subtilisin A as a main component, and arazyme is a metalloprotease produced from microorganisms isolated from the intestines of Korean unsweetened spiders. Each is well known in this field.

In the present invention, the amount of proteolytic enzyme used may be adjusted according to, for example, the amount of a mixture in which chicken liver and pig liver are added to the raw material to be proteolyzed, that is, slaughtered by-products. For example, when using alcalase and arazyme as proteolytic enzymes, preferably 0.1 to 5 parts by weight of alcalase based on 1000 parts by weight of a mixture of chicken liver and pig liver added to slaughtering by-products (based on dry weight) and 0.1 to 5 parts by weight of arazyme (based on dry weight) are used. More preferably, based on 1000 parts by weight of a mixture in which chicken liver and pig liver are added to the slaughtering by-product, 0.2 to 3 parts by weight of alkalinease and 0.2 to 3 parts by weight of arazyme, more preferably 0.3 to 2 parts by weight of alkalinease and ara 0.2 to 1.5 parts by weight of zyme, more preferably 0.5 to 1.5 parts by weight of alcalase and 0.3 to 1 part by weight of arazyme, more preferably 0.6 to 1 part by weight of alcalase and 0.3 to 0.7 parts by weight of arazyme .

In the present invention, the protein decomposition temperature and time is preferably 55 to 65 ℃, 3 to 20 hours. More preferably, it is 57-63 degreeC, 4 to 18 hours, More preferably, it is set as 59-61 degreeC and 5 to 15 hours.

The production method of the present invention preferably further comprises the step of adding phosphoric acid and a natural antioxidant to the protein degradation product.

In the present invention, the step of adding the phosphoric acid and natural antioxidant can be carried out by using an apparatus or device commonly used for mixing raw materials of food or feed, for example, a blender, and is used to perform the protein degradation. It can also be carried out in a reaction tank. Preferably, a temperature control device is provided to maintain a constant temperature, and a device capable of stirring is used with a screw or the like.

At this time, preferably, the phosphoric acid and natural antioxidant are added and the reaction is further maintained for 5 to 12 hours, more preferably 6 to 10 hours, more preferably 7 to 9 hours. The temperature at this time is preferably 55 to 65°C, more preferably 57 to 63°C, and still more preferably 59 to 61°C.

In the case of further maintaining the reaction including adding the phosphoric acid and natural antioxidant as described above, the protein degradation time in the step of obtaining the protein degradation product is preferably 3 to 10 hours, more preferably 4 to 8 hours, more preferably 5 to 7 hours.

Phosphoric acid in the present invention is preferably added so as to be 3 to 6% by weight of phosphoric acid based on the weight of the final feed additive, more preferably 4 to 5% by weight, more preferably 4 to 4.5% by weight. do.

As the natural antioxidant in the present invention, tocopherol concentrate, rosemary extract, or a mixture thereof is preferably used. In this case, the tocopherol concentrate is preferably obtained by vacuum steam distillation of edible vegetable oil, and is a form of vitamin E containing concentrated tocopherol, more preferably INS No. 307b is a food additive. The rosemary extract is preferably extracted from Rosmarinus officinalis L. , more preferably INS (International Numbering System for Food Additives) No. It is a food additive of 392. More preferably, a natural antioxidant consisting of the tocopherol concentrate, rosemary extract, lecithin and soybean oil and containing 5 to 10 g of tocopherol per 1 kg is used.

In the present invention, the natural antioxidant is preferably added in an amount of 1 to 4 parts by weight based on 400 parts by weight of the slaughtering by-product, more preferably 1 to 3 parts by weight, more preferably 1.5 to 2.5 parts by weight.

The feed additive of the present invention may be the protein degradation product itself obtained in the step of obtaining the protein degradation product, or it may be one obtained through the step of adding the phosphoric acid and natural antioxidant.

The feed additive of the present invention prepared by the manufacturing method as described above may be in a liquid form or in a powder form. In this case, the powdery form can be obtained by a method of pulverizing the liquid form, for example, concentration under reduced pressure or freeze-drying.

The feed of the present invention is characterized in that the feed additive of the present invention as described above is added. At this time, preferably, 1 to 10 parts by weight of the feed additive of the present invention is added based on 100 parts by weight of the feed before the basic feed, that is, the feed additive of the present invention is added, more preferably 1 to 5 parts by weight, more preferably is added in an amount of 2 to 4 parts by weight. The basic feed is preferably a feed containing corn, meat meal and soybean meal as main components, for example, a feed in which corn, meat meal and soybean meal account for 70% or more of the feed weight, and more preferably a feed containing animal fats and oils. . More preferably, it is a feed containing 40 to 50% by weight of corn, 20 to 30% by weight of meat meal, 15 to 25% by weight of soybean meal, and 5 to 15% by weight of animal oil and fat, and more preferably, corn, meat meal while satisfying the above content. , soybean meal and animal fat and oil.

In addition, the present invention provides a feed manufacturing method for adding the feed additive of the present invention to the basic feed. In this case, the preferred addition amount of the feed additive of the present invention and matters relating to the basic feed are as described above.

In the present invention, the companion animal is preferably a dog.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and therefore, the scope of the present invention is not to be construed as being limited by these examples.

[Example]

Example 1. Preparation of feed additive

400 kg of slaughtered by-products inedible part (intestinal and fat), 300 kg of chicken liver, and 300 kg of pork liver were crushed and put into the reactor, followed by proteolytic enzyme alcalase® (NOVOZYMES A/S) 800 g and arazyme® ) (Insect Biotech Co., Ltd.) 500 g was added and heated, and the proteolysis reaction proceeded for 6 hours after the temperature of the reactor reached 60°C.

After the decomposition reaction, 85% (w/w) phosphoric acid solution was added at 5% (w/w), and 2.0 kg of natural antioxidant (NaSure 51) containing tocopherol (α, βδγ and rosemary oil) was added at 8 The reaction was further maintained for a period of time, and then filtered to prepare a feed additive from which solids were removed.

The natural antioxidant is INS No. 392 Rosemary Extract, INS No. 307b tocopherol concentrate, INS No. It consists of 322(i) lecithin and soybean oil, and contains about 7 g of tocopherol per 1 kg.

Comparative Example 1. Existing slaughter by-product protein degradation product

For comparison, the protein degradation product of Korea Patent No. 10-0394581 was used in the experiment.

Specifically, 1000 kg of slaughtered by-products inedible parts (internal organs, fat, head, feet) were put into the reaction tank, and then 250 g of proteolytic enzyme alcalase® was added and heated, so that the temperature of the reaction tank was about 60 ° C. Proteolytic reaction proceeds for 3 hours after reaching, and after the degradation reaction, 50% (w/w) phosphoric acid solution is added at 5% (w/w), and 1.5 kg of sodium propionate is added as an antifungal agent. It is prepared by adding

Example 2. Manufacture of feed additive to feed

3 parts by weight of the feed additive of Example 1 was added to 100 parts by weight of a feed (Daejoo Industrial Co., Ltd.) consisting of 45% by weight of corn, 25% by weight of meat meal, 20% by weight of soybean meal, and 10% by weight of animal fat.

Comparative Example 2. Manufacture of feed with the addition of an existing slaughtering by-product protein degradation product

It was carried out in the same manner as in Example 2, except that the protein degradation product of Comparative Example 1 was used instead of the feed additive of Example 1.

Experimental Example 1. Efficacy evaluation of feed additives

At the dog breeding test site, each dog was placed in a cage with two feeders, and after feeding the feed of Example 2 or Comparative Example 2 at a set time every day for 30 minutes, the remaining amount of feed remaining in the container was investigated. At this time, water was freely ingested with a nipple-type drinker.

The results are shown in Tables 1 and 2.

Experimental results for small dogs number of experiments Feeding amount (g) Feed intake (g) Remaining amount after intake (g) Comparative Example 2 Example 2 Comparative Example 2 Example 2 Comparative Example 2 Example 2 One 6,250 6,250 483 1,762 5,767 4,488 2 6,250 6,250 717 1,567 5,533 4,683 3 6,250 6,250 1,033 1,609 5,217 4,641 4 6,250 6,250 558 1,719 5,692 4,531 5 6,250 6,250 817 1,636 5,433 4,614 6 6,250 6,250 517 1,794 5,733 4,456 7 6,250 6,250 550 1,742 5,700 4,508 total 43,750 43,750 4,675 11,829 39,075 31,921

* Experimental dogs: 5 small dogs weighing around 5kg* Experimental period: 5 days

As a result of the experiment for small dogs, as shown in Table 1, the feed intake (11,829 g) of Example 2 was 253% higher than that of Comparative Example 2 (4,675 g).

Experimental results for medium-sized dogs number of experiments Feeding amount (g) Feed intake (g) Remaining amount after intake (g) Comparative Example 2 Example 2 Comparative Example 2 Example 2 Comparative Example 2 Example 2 One 17,500 17,500 1,517 4,415 15,983 13,085 2 17,500 17,500 2,730 4,177 14,770 13,323 3 17,500 17,500 1,873 4,085 15,627 13,415 4 17,500 17,500 1,595 4,541 15,905 12,959 5 17,500 17,500 1,488 4,513 16,012 12,987 6 17,500 17,500 1,709 4,712 15,791 12,788 7 17,500 17,500 1,616 4,626 15,884 12,874 total 122,500 122,500 12,528 31,069 109,972 91,431

* Test dogs: 7 medium-sized dogs weighing around 20 kg* Experiment period: 5 days

As a result of the experiment for medium-sized dogs, as shown in Table 2, the feed intake (31,069 g) of Example 2 was 248% higher than that of Comparative Example 2 (12,528 g).

Claims (4)

A method for producing a companion animal feed additive comprising the step of proteolytically decomposing a mixture of chicken liver and pig liver added to a slaughtering by-product with a proteolytic enzyme to obtain a protein degradation product. The method of claim 1,
The step of obtaining the protein degradation product is a mixture of 200 to 400 parts by weight of chicken liver and 200 to 400 parts by weight of pig liver added to 400 parts by weight of slaughtering by-products with alkalinease and arazyme at 55 to 65° C. for 3 to 20 hours. A method of decomposing a protein to obtain a protein degradation product. A companion animal feed additive manufactured by the method of claim 1 or 2. A feed comprising the companion animal feed additive of claim 3.

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