KR20220071701A - Composition for softening meat and method for softening meat using the same - Google Patents

Composition for softening meat and method for softening meat using the same Download PDF

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KR20220071701A
KR20220071701A KR1020200159135A KR20200159135A KR20220071701A KR 20220071701 A KR20220071701 A KR 20220071701A KR 1020200159135 A KR1020200159135 A KR 1020200159135A KR 20200159135 A KR20200159135 A KR 20200159135A KR 20220071701 A KR20220071701 A KR 20220071701A
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meat
softening
carbonate
composition
phosphate
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황영
권선향
이세표
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주식회사 아워홈
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/42Additives other than enzymes or microorganisms in meat products or meat meals
    • A23L13/428Addition of flavours, spices, colours, amino acids or their salts, peptides, vitamins, yeast extract or autolysate, nucleic acid or derivatives, organic acidifying agents or their salts or acidogens, sweeteners, e.g. sugars or sugar alcohols; Addition of alcohol-containing products
    • AHUMAN NECESSITIES
    • A22BUTCHERING; MEAT TREATMENT; PROCESSING POULTRY OR FISH
    • A22CPROCESSING MEAT, POULTRY, OR FISH
    • A22C9/00Apparatus for tenderising meat, e.g. ham
    • A22C9/004Apparatus for tenderising meat, e.g. ham by massaging
    • A22C9/005Tumblers and rotating drums for massaging meat in their interior
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/48Addition of, or treatment with, enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/70Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor
    • A23L13/72Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor using additives, e.g. by injection of solutions
    • A23L13/74Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor using additives, e.g. by injection of solutions using microorganisms or enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P20/00Coating of foodstuffs; Coatings therefor; Making laminated, multi-layered, stuffed or hollow foodstuffs
    • A23P20/10Coating with edible coatings, e.g. with oils or fats

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
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  • Meat, Egg Or Seafood Products (AREA)

Abstract

The present invention relates to a tenderizer composition including 96.0 to 98.0 wt% of alkali salt and 2 to 4 wt% of a proteolytic breakdown enzyme and a tenderizing method of meat using the same. When meat is tenderized by using the tenderizer composition according to the present invention, a tenderizing effect is excellent, there is no bad smell/taste, there is no powdering phenomenon due to excessive tenderizing of a meat surface, and there are effects of having high tenderizing uniformity and excellent functional quality.

Description

육류 연화용 조성물 및 이를 이용하여 육류를 연화시키는 방법{Composition for softening meat and method for softening meat using the same} Composition for softening meat and a method for softening meat using the same

본 발명은 육류 연화용 조성물 및 이를 이용하여 육류를 연화시키는 방법에 관한 것으로, 더욱 상세하게는 저작, 연하 및 소화 능력이 저하된 어린이, 환자 또한 고령자들의 육류 섭취 편의성을 향상시키기 위한 육류의 연화 조성물 및 육류 연화 방법에 관한 것이다.The present invention relates to a composition for softening meat and a method for softening meat using the same, and more particularly, to a composition for softening meat for improving the convenience of eating meat for children, patients, and the elderly with reduced chewing, swallowing and digestive abilities and to a method of tenderizing meat.

여기서는, 본 개시에 관한 배경기술이 제공되며, 이들이 반드시 공지기술을 의미하는 것은 아니다.Background to the present disclosure is provided herein, which does not necessarily imply known art.

연하장애는 음식물을 삼키거나 입에서 위장까지 이동할 수 있도록 구강상태를 준비시키는데 어려움이 있는 것을 의미한다. 이와 같은 문제가 발생하는 경우 식사 중 또는 식사 후 기침 및 질식, 음식이 달라붙음, 침 흘림, 영양 결핍 등의 증세가 나타날 수 있다. 또한, 저작능력이 떨어지는 어린이, 환자, 고령자들의 경우 제대로 분쇄되지 않은 음식물이 소화기관에 무리를 주거나 저작이 편한 음식물만 섭취하게 되기 때문에 영양의 불균형 역시 발생하기 쉽다.Dysphagia means difficulty in swallowing food or preparing the oral cavity for movement from the mouth to the stomach. When these problems occur, symptoms such as coughing and choking during or after eating, food sticking, drooling, and nutritional deficiencies may appear. In addition, in the case of children, patients, and the elderly with poor chewing ability, food that is not properly crushed puts a strain on the digestive system or eats only foods that are easy to chew, so nutritional imbalance is also likely to occur.

특히, 육류의 경우 환자나 어린이, 기력이 떨어진 고령자에게 양질의 단백질을 공급할 수 있는 식재료이나 저작, 연하, 소화에 어려움이 있을 수 있으므로 육류를 분쇄하여 재구성하는 공법 등을 이용하여 소화와 섭취에 용이한 형태로의 전환을 시도하고 있다. 그러나, 고기 자체의 맛을 즐기기 위해서는 고기의 형태와 조직을 유지하면서도 경도를 감소시켜 육류의 기호도를 높일 필요가 있으며, 육류를 연화하기 위한 다양한 기술이 개발되고 있다.In particular, meat is a food material that can supply high-quality protein to patients, children, and the elderly with low energy, but there may be difficulties in mastication, swallowing, and digestion. Attempts to transform into one form. However, in order to enjoy the taste of meat itself, it is necessary to increase the taste of meat by reducing hardness while maintaining the shape and structure of the meat, and various technologies for softening meat have been developed.

이에 따라, 어린이, 환자나 고령자의 육류 섭취 편의성을 높일 뿐 아니라, 선호도가 낮은 육류의 질긴 부위의 기호도를 높일 수 있는 육류의 연화 방법의 개발이 필요한 실정이다.Accordingly, there is a need to develop a meat softening method that can increase the convenience of eating meat for children, patients, or the elderly, as well as increase the preference for tough parts of meat with low preference.

한편, 단백질 분해효소는 육류 연화에 대한 효과적이지만, 단독 사용 시 연화 불균형, 과다연화 등의 문제점들이 있다. 물리적 및 화학적인 방법은 연화 균일성, 과대연화 방지에 우수하였으나, 효소대비 연화력이 약하며, 과대 사용 시 관능적인 품질 저하 등 문제들이 있어 안정하고 효과적인 연화방법이 요구되고 있다.On the other hand, although proteolytic enzymes are effective for tenderizing meat, there are problems such as softening imbalance and over-softening when used alone. Physical and chemical methods were excellent in softening uniformity and prevention of over-softening, but they have weak softening power compared to enzymes, and there are problems such as sensory quality deterioration when over-used, so a stable and effective softening method is required.

본 발명은 상기와 같은 문제점을 해결하기 위하여 효소적, 화학적 및 물리적 방법을 용합한 기술을 이용한 육류 연화 조성물 및 이를 이용하여 육류를 연화시키는 방법을 제공하고자 한다. An object of the present invention is to provide a meat softening composition using a technology that combines enzymatic, chemical and physical methods in order to solve the above problems, and a method for softening meat using the same.

그러나 본 발명의 목적들은 상기에 언급된 목적으로 제한되지 않으며, 언급되지 않은 또 다른 목적들은 아래의 기재로부터 당업자에게 명확하게 이해될 수 있을 것이다.However, the objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned will be clearly understood by those skilled in the art from the following description.

본 발명은 알칼리염 96.0 내지 98.0 wt% 및 단백질 분해효소 2 내지 4 wt% 포함하는 것을 특징으로 하는 연화제 조성물을 제공한다.The present invention provides an emollient composition comprising 96.0 to 98.0 wt% of an alkali salt and 2 to 4 wt% of a proteolytic enzyme.

또한 상기 알칼리염은 탄산염 및 인산염 중 어느 1종 이상을 포함하고, 상기 탄산염은 탄산수소나트륨 및 탄산나트륨 중 어느 1종 이상을 포함하는 것을 특징으로 한다.In addition, the alkali salt includes any one or more of carbonate and phosphate, and the carbonate is characterized in that it includes any one or more of sodium hydrogen carbonate and sodium carbonate.

또한 상기 단백질 분해효소는 파파인(Papain) 및 브로멜라인(Bromelain) 중 어느 1종 이상을 포함하는 것을 특징으로 한다.In addition, the proteolytic enzyme is characterized in that it contains any one or more of papain (Papain) and bromelain (Bromelain).

또한 본 발명은 연화제 조성물을 이용하여 원육을 연화시키는 연화육 제조방법으로서, 원육 100 중량부에 대하여 상기 연화제 조성물을 0.1 내지 1 중량부 사용하는 것을 특징으로 하는 연화육 제조방법을 제공한다.The present invention also provides a method for preparing tenderized meat for softening raw meat using a softener composition, wherein 0.1 to 1 part by weight of the softening agent composition is used with respect to 100 parts by weight of the raw meat.

또한 원육에 상기 연화제 조성물을 첨가하고 진공조건에서 텀블링하여 균질화한 후 진공 포장하여 20 내지 25 시간 동안 냉장 숙성시키는 것을 특징으로 한다.In addition, the softening agent composition is added to the raw meat, homogenized by tumbling under vacuum conditions, and then vacuum-packed and refrigerated for 20 to 25 hours.

본 발명에 따른 연화 조성물을 사용하여 육류를 연화시키면 연화효과가 우수하며, 이취/이미가 없고, 고기 표면 과대연화로 인한 가루화 현상이 없으며, 연화 균일성 높고 관능적인 품질이 우수한 효과를 제공한다. When meat is softened using the softening composition according to the present invention, the softening effect is excellent, there is no odor/flavor, there is no pulverization phenomenon due to over-softening of the meat surface, and the softening uniformity and sensual quality are excellent. .

도 1 및 2는 비효소 단일 연화소재에 따른 연화력을 측정한 결과를 나타낸 것이다.
도 3은 탄산염과 당을, 도 4는 탄산염과 인산염을, 도 5는 탄산염과 브로멜라인을, 도 6은 탄산염과 파파인을, 도 7은 알칼리염과 단백질 분해효소를 혼합한 연화소재에 따른 연화력을 측정한 결과를 나타낸 것이다.
1 and 2 show the results of measuring the softening force according to the non-enzymatic single softening material.
3 is carbonate and sugar, FIG. 4 is carbonate and phosphate, FIG. 5 is carbonate and bromelain, FIG. 6 is carbonate and papain, and FIG. 7 is a softening material mixed with alkali salt and proteolytic enzyme The result of measuring the softening power is shown.

본 명세서에 사용되는 모든 기술용어 및 과학용어는 다른 언급이 없는 한은 기술적으로 통상의 기술을 가진 자에게 일반적으로 이해되는 것과 동일한 의미를 가진다. 또한 본 명세서 및 청구범위의 전반에 걸쳐, 다른 언급이 없는 한 포함(comprise, comprises, comprising)이라는 용어는 언급된 물건, 단계 또는 일군의 물건, 및 단계를 포함하는 것을 의미하고, 임의의 어떤 다른 물건, 단계 또는 일군의 물건 또는 일군의 단계를 배제하는 의미로 사용된 것은 아니다.All technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art, unless otherwise stated. Also throughout this specification and claims, unless otherwise indicated, the term comprise, comprises, comprising is meant to include the recited object, step or group of objects, and steps, and any other It is not used in the sense of excluding an object, step, or group of objects or groups of steps.

이하에 본 발명을 상세하게 설명하기에 앞서, 본 명세서에 사용된 용어는 특정의 실시예를 기술하기 위한 것일 뿐 첨부하는 특허청구의 범위에 의해서만 한정되는 본 발명의 범위를 한정하려는 것은 아님을 이해하여야 한다.Prior to describing the present invention in detail below, it is to be understood that the terminology used herein is for the purpose of describing specific embodiments and is not intended to limit the scope of the present invention, which is limited only by the appended claims. shall.

한편, 본 발명의 여러 가지 실시예들은 명확한 반대의 지적이 없는 한 그 외의 어떤 다른 실시예들과 결합될 수 있다. 특히 바람직하거나 유리하다고 지시하는 어떤 특징도 바람직하거나 유리하다고 지시한 그 외의 어떤 특징 및 특징들과 결합될 수 있다. 이하, 첨부된 도면을 참조하여 본 발명의 실시예 및 이에 따른 효과를 설명하기로 한다.On the other hand, various embodiments of the present invention may be combined with any other embodiments unless clearly indicated to the contrary. Any feature indicated as particularly preferred or advantageous may be combined with any other feature and features indicated as preferred or advantageous. Hereinafter, embodiments of the present invention and effects thereof will be described with reference to the accompanying drawings.

본 발명의 일실시예에 따른 육류 연화제 조성물은 알칼리염 및 단백질 분해효소를 포함하여 육류의 관능적 품질이 유지되며, 이취/이미가 없고 고기 표면 과대연화로 인한 가루화 현상이 없지만, 전반적인 연화효과가 우수한 효과를 제공한다. The meat tenderizer composition according to an embodiment of the present invention maintains the organoleptic quality of meat, including alkali salts and proteolytic enzymes, has no off-flavor/flavor, and does not have pulverization due to over-softening of the meat surface, but has an overall softening effect. It provides an excellent effect.

상기 연화제 조성물은 알칼리염을 96.0 내지 98.0 wt%, 단백질 분해효소를 2 내지 4 wt% 포함한다. 알칼리염을 단독으로 사용하는 경우 연화제 조성물을 고농도로 사용하여야 연화력을 가지는데, 이취(암모니아 냄새) 및 변색(진한 갈색) 등 관능적 저하 현상이 함께 발생하는 문제점이 있다. 또한 단백질 분해효소만을 단독으로 사용하는 경우 고기 표면의 과대 연화현상이 나타나는 문제점이 있다. The emollient composition includes 96.0 to 98.0 wt% of an alkali salt and 2 to 4 wt% of a proteolytic enzyme. When an alkali salt is used alone, a softening agent composition must be used in a high concentration to have softening power, but there is a problem in that sensory deterioration such as off-flavor (ammonia odor) and discoloration (dark brown) occur together. In addition, when only proteolytic enzymes are used alone, there is a problem in that the surface of meat is over-softened.

본 발명은 알칼리염과 단백질 분해효소를 특정 비율로 혼합하여 사용함으로써 연화력 상승효과를 가지면서도 고기 표면의 과대 연화현상이 개선되어 관능적 품질 또한 우수한 효과를 제공한다. 상기 연화제 조성물은 더욱 바람직하게는 알칼리염을 97.0 내지 98.0 wt%, 단백질 분해효소를 2 내지 3 wt% 포함하는 것이 좋다.In the present invention, by using a mixture of alkali salt and proteolytic enzyme in a specific ratio, while having a synergistic effect on softening power, over-softening of the meat surface is improved, thereby providing excellent sensory quality. The emollient composition more preferably contains 97.0 to 98.0 wt% of an alkali salt and 2 to 3 wt% of a proteolytic enzyme.

상기 알칼리염은 탄산염 및 인산염 중 어느 1종 이상을 포함하며, 상기 탄산염은 탄산수소나트륨 및 탄산나트륨 중 어느 1종 이상을 포함한다. 상기 연화제 조성물은 알칼리염으로 바람직하게는 탄산염을 포함하며, 더욱 바람직하게는 탄산나트륨을 포함한다. The alkali salt includes any one or more of carbonate and phosphate, and the carbonate includes any one or more of sodium hydrogen carbonate and sodium carbonate. The emollient composition preferably includes carbonate as an alkali salt, and more preferably includes sodium carbonate.

상기 단백질 분해효소는 식물성 단백질 분해효소를 사용하며, 바람직하게는 파파인(Papain) 및 브로멜라인(Bromelain) 중 어느 1종 이상을 사용하는 것이 좋고, 더욱 바람직하게는 파파인을 사용하는 것이 좋다. The proteolytic enzyme uses a plant protease, preferably any one or more of papain and bromelain, and more preferably papain.

본 발명에 따른 연화제 조성물을 사용하여 연화육을 제조하는 방법은 고기 100 중량부에 대하여 상기 연화제 조성물을 0.1 내지 1 중량부 사용한다. 연화제 조성물을 0.1 중량부 미만으로 사용하는 경우 연화력이 떨어지며, 1 중량부 초과하여 사용 시 연화력은 높아지지만 이취(암모니아 냄새) 및 변색(진한 갈색) 등 관능적 저하 현상이 나타나는 문제점이 있다. 바람직하게는 고기 100 중량부에 대하여 상기 연화제 조성물을 0.3 내지 0.5 중량부 사용하는 것이 좋다. 더욱 바람직하게는 고기 100 중량부에 대하여 상기 연화제 조성물을 0.4 내지 0.5 중량부 사용하는 것이 좋다.In the method for preparing tenderized meat using the softener composition according to the present invention, 0.1 to 1 part by weight of the softener composition is used based on 100 parts by weight of the meat. When the softening agent composition is used in an amount of less than 0.1 parts by weight, the softening power is lowered, and when used in an amount exceeding 1 part by weight, the softening power is increased, but there is a problem in that sensory deterioration such as off-flavor (ammonia smell) and discoloration (dark brown) appears. Preferably, 0.3 to 0.5 parts by weight of the softening agent composition is used based on 100 parts by weight of the meat. More preferably, 0.4 to 0.5 parts by weight of the softening agent composition is used based on 100 parts by weight of the meat.

연화육을 제조하는 더욱 구체적인 방법은 원육에 연화제 조성물을 첨가하고 진공조건에서 텀블링하여 균질화한 후 진공 포장하여 20 내지 25 시간 냉장(2 내지 6℃) 숙성시킴으로써 연화육을 제조한다. 상기 연화제 조성물은 pH 5 정도의 약산성을 띄는 소스에 첨가되었을 때 8.5 내지 8.8 으로 pH를 상승시키는 효과를 갖는다.A more specific method for preparing tenderized meat is to prepare tenderized meat by adding a softening agent composition to raw meat, tumbling under vacuum conditions, homogenizing, vacuum packaging, and refrigeration (2 to 6° C.) aging for 20 to 25 hours. The emollient composition has an effect of raising the pH to 8.5 to 8.8 when added to a slightly acidic source of about pH 5.

실시예 및 실험예Examples and Experimental Examples

(1) 비효소 단일 연화소재 스크리닝(1) Non-enzyme single softening material screening

육류 단백질 연화에 관련한 비효소 성분의 육류에 대한 연화효과를 평가하였다. 소재 후보군은 탄산염 2종(탄산나트륨, 탄산수소나트륨), 복합인산염 1종(폴리인산나트륨/메타인산나트륨의 혼합한 복합인산염), 당알코올 2종(소르비톨, 말티톨), 당류 1종(트레할로스), 칼슘염 2종(연화칼슘 및 젖산칼슘), 유화제 1종(MCT 오일)으로 구성되었다.The softening effect of non-enzyme ingredients related to meat protein softening on meat was evaluated. The material candidates are 2 types of carbonate (sodium carbonate, sodium bicarbonate), 1 type of complex phosphate (composite phosphate of sodium polyphosphate/sodium metaphosphate), 2 types of sugar alcohols (sorbitol, maltitol), 1 type of sugar (trehalose), It consisted of two types of calcium salts (emulsified calcium and calcium lactate) and one type of emulsifier (MCT oil).

본 실험에서는 호주산 두께 3mm인 소불고기에 대한 연화 효과를 측정하였으며, 연화육 제조에 간장불고기양념소스를 이용하였다. In this experiment, the softening effect of beef bulgogi with a thickness of 3 mm from Australia was measured, and soy sauce bulgogi sauce was used to prepare the tenderized meat.

양념육 제조는 다음 순서대로 진행되었다. 슬라이스된 냉동 원육을 해동시키고, 정제수에 조건에 따른 각각 소재를 첨가하고 소재를 물에 녹인 후 소스와 혼합시켰다. 슬라이스 원료육 및 소스를 텀블러에 투입하여 진공조건에서 5~10분 간 텀블링하였다. 진공 텀블러로 배합한 후, 폴리백에 포장하여 24시간 냉장(5℃) 숙성 후 -18℃ 냉동고에서 냉동하였다. The seasoning was carried out in the following order. The sliced frozen raw meat was thawed, each material according to the conditions was added to purified water, and the material was dissolved in water and then mixed with the sauce. The sliced raw meat and sauce were put into a tumbler and tumbled for 5 to 10 minutes under vacuum conditions. After mixing with a vacuum tumbler, it was packaged in a poly bag, aged in refrigeration (5°C) for 24 hours, and then frozen in a -18°C freezer.

냉동된 양념육을 1~1.5시간 침수 해동 혹은 24시간 냉장 해동으로 완전 해동 후 조리하였다. 예열된 볶음팬에 양념육을 넣어 센 불로 4분간 조리하였으며, 조리 후 관능 및 물성평가를 진행하였다. 고기 중량 대비 탄산염, 인산염 또한 칼슘염 0.4 % ~ 1.6%, 당류 및 MCT 오일 10% 사용 시의 연화효과를 측정하였다. Frozen seasoned meat was completely thawed by immersion thawing for 1-1.5 hours or refrigerated thawing for 24 hours before cooking. Seasoned meat was put in a preheated frying pan and cooked over high heat for 4 minutes, and sensory and physical properties were evaluated after cooking. The softening effect was measured when using 0.4% to 1.6% of carbonate, phosphate, calcium salt, sugar, and 10% of MCT oil relative to the weight of meat.

제조된 양념육의 관능평가 실험군 당 샘플 5개 이상 시식 후 1(질김), 2(조금 질김), 3(조금 부드러움), 4(부드러움), 5(매우 부드러움)로 나누어 연화도를 평가하였다. 연화도 4 수준 이상을 연화식 조건으로 선정하여 평가하였고, 맛/향을 질적 표현으로 기록하였으며, 외관(부서짐 현상) 및 육즙 손실의 양은 육안으로 관찰 후 사진으로 기록하였다. Sensory evaluation of the prepared seasoned meat After tasting at least 5 samples per experimental group, the softness was evaluated by dividing the samples into 1 (tough), 2 (slightly tough), 3 (slightly soft), 4 (soft), and 5 (very soft). A softening level of 4 or higher was selected and evaluated as a softening condition, and taste/odor was recorded as a qualitative expression, and the appearance (breaking phenomenon) and amount of loss of juice were observed with the naked eye and then recorded with a photograph.

연화육의 물성 측정은 KS 고령친화식품 품질 기준의 경도 측정 방법을 따라 진행하였다. 구체적인 방법은 다음과 같다. 지름 3mm 원형 프로브를 이용하여 압축속도 10mm/sec, 클리어런스를 시료의 두께 30%, 시료 온도 20±2℃ 조건에서 5회 측정한다. The physical properties of the tenderized meat were measured according to the hardness measurement method of the KS age-friendly food quality standard. The specific method is as follows. Using a 3mm diameter circular probe, measure the compression speed of 10mm/sec, the clearance at 30% of the thickness of the sample, and the sample temperature at 20±2℃ 5 times.

그 결과를 표 1, 2 및 도 1, 2에 나타내었다. The results are shown in Tables 1 and 2 and FIGS. 1 and 2 .

실험군 experimental group 맛&향 taste & aroma 경도 Hardness 외관(색) Appearance (color) 대조군 control 불고기 고유의 맛 Unique taste of bulgogi 1 (질김)1 (tough) 불고기 고유의 색(Brown) Bulgogi's unique color (Brown) 탄산나트륨 0.4%sodium carbonate 0.4% 불고기 고유의 맛 Unique taste of bulgogi 1 (질김)1 (tough) 불고기 고유의 색(Brown) Bulgogi's unique color (Brown) 탄산나트륨 0.8%Sodium carbonate 0.8% 약한 암모니아 냄새weak ammonia odor 4 (부드러움)4 (soft) 진한 갈색
(Dark Brown))
dark brown
(Dark Brown))
탄산나트륨 1.6 %Sodium carbonate 1.6% 강한 암모니아 냄새strong ammonia smell 5
(매우 부드러움)
5
(very soft)
진한 갈색
(Dark Brown)
dark brown
(Dark Brown)
탄산수소나트륨0.4%Sodium bicarbonate 0.4% 불고기 고유의 맛 Unique taste of bulgogi 1 (질김)1 (tough) 불고기 공유의 색 (Brown)Color of Bulgogi Sharing (Brown) 탄산수소나트륨0.8%Sodium bicarbonate 0.8% 약한 암모니아 냄새weak ammonia odor 5
(매우 부드러움)
5
(very soft)
진한 갈색
(Dark Brown))
dark brown
(Dark Brown))
탄산수소나트륨1.6 %Sodium bicarbonate 1.6% 강한 암모니아 냄새strong ammonia smell 5
(매우 부드러움)
5
(very soft)
진한 갈색
(Dark Brown)
dark brown
(Dark Brown)
인산염 0.4%Phosphate 0.4% 불고기 고유의 맛 Unique taste of bulgogi 1 (질김)1 (tough) 불고기 고유의 색
(Brown)
Bulgogi's unique color
(Brown)
인산염 0.8%Phosphate 0.8% 불고기 고유의 맛 Unique taste of bulgogi 인산염 1.6%Phosphate 1.6% 불고기 고유의 맛 Unique taste of bulgogi 연화칼슘 0.4%Softened calcium 0.4% 약한 쓴맛mild bitter taste 연화칼슘 0.8%Softened calcium 0.8% 강한 쓴맛strong bitter taste 젖산칼슘 0.4%Calcium Lactate 0.4% 약한 쓴맛mild bitter taste 젖산칼슘 0.8%Calcium Lactate 0.8% 약한 쓴맛 mild bitter taste 솔르비톨 10%Sorbitol 10% 약한 단맛mild sweetness 말티톨 10%Maltitol 10% 단맛sweetness 트레할로스 10%Trehalose 10% 약한 단맛mild sweetness MCT 오일 10%MCT Oil 10% 불고기 고유의 맛Unique taste of bulgogi

소재Material 사용량(% 고기대비)Consumption (% compared to meat) 경도결과(N/m2)Hardness result (N/m 2 ) 없음(대조군)None (control) -- 576,516576,516 탄산나트륨sodium carbonate 0.40.4 536,212536,212 0.80.8 376,761 376,761 1.61.6 310,824 310,824 탄산수소나트륨sodium bicarbonate 0.40.4 506,420506,420 0.80.8 301,525 301,525 1.61.6 254,019 254,019 인산염phosphate 0.40.4 584,462584,462 0.80.8 562,793562,793 1.61.6 535,618 535,618 염화칼슘calcium chloride 0.40.4 576,392576,392 0.80.8 521,980 521,980 젖산칼슘calcium lactate 0.40.4 631,925631,925 0.80.8 540,593 540,593 소르비톨sorbitol 1010 747,147747,147 말티톨maltitol 1010 542,790542,790 트레할로스Trehalose 1010 649,091649,091 MCT 오일 MCT Oil 1010 581,684581,684

표 2 및 도 1, 도 2에 나타난 것과 같이 기계적 경도에서, 9개 후보소재 중 탄산염이 유일한 연화효과가 있는 소재로 확인되었다. 그러나 탄산염 0.8% 이상 사용 시 높은 연화력이 확인되었으나, 이취(암모니아 냄새) 및 변색(진한 갈색) 등 관능적 저하 현상이 발견되었다. As shown in Table 2 and FIGS. 1 and 2, in terms of mechanical hardness, carbonate was confirmed as the only material having a softening effect among the nine candidate materials. However, when more than 0.8% of carbonate was used, high softening power was confirmed, but sensory deterioration such as off-flavor (ammonia odor) and discoloration (dark brown) was found.

(2) 연화소재 조합별 평가 (2) Evaluation by soft material combination

화학성분 중 연화효과가 있는 탄산염의 경우, 저농도는 연화력이 약하며, 고농도 사용 시 연화력이 강하나 고기의 관능적 품질 저하를 일으키고 사용하는데 제한성이 있는 것을 확인하였으므로 탄산염과 다른 성분(당질, 인산염, 단백질 분해효소)을 조합하여 연화 시너지 연화력이 우수한 최적 조합이 도출하였다. In the case of carbonate, which has a softening effect among chemical components, it has been confirmed that the softening power is weak at low concentration, and the softening power is strong when used at high concentration, but it causes a decrease in the sensory quality of meat and limits its use. synergistic softening enzyme), an optimal combination with excellent softening power was derived.

탄산염과 당질(소르비톨 S, 말티톨 M, 트레할로스 T)을 조합하여 연화력 측정한 결과를 표 3 및 도 3에 나타내었다. 표 3 및 도 3에 나타난 것과 같이 탄산염과 당질의 조합은 연화력에 대한 유의미한 상승효과가 없는 것을 알 수 있다. Table 3 and FIG. 3 show the results of measuring softening power by combining carbonate and carbohydrates (sorbitol S, maltitol M, and trehalose T). As shown in Table 3 and Figure 3, it can be seen that the combination of carbonate and saccharide does not have a significant synergistic effect on softening power.

소재Material 사용량(% 고기대비)Consumption (% compared to meat) 경도결과(N/m2)Hardness result (N/m 2 ) 없음(대조군)None (control) -- 562,300562,300 탄산염 lead carbonate 0.40.4 496,811496,811 0.80.8 349,864 349,864 1.61.6 348,753 348,753 탄산염 + 솔르비톨Carbonate + Sorbitol 0.4/100.4/10 544,760544,760 0.8/100.8/10 389,422389,422 1.6/101.6/10 310,794310,794 탄산염 + 말티톨carbonate + maltitol 0.4/100.4/10 622,432622,432 0.8/100.8/10 380,575380,575 1.6/101.6/10 269,703269,703 탄산염 + 트레할로스carbonate + trehalose 0.4/100.4/10 462,045462,045 0.8/100.8/10 386,041386,041 1.6/101.6/10 361,835361,835

탄산염과 인산염을 조합하여 연화력 측정한 결과를 표 4 및 도 4에 나타내었고, 관능평가 결과를 표 5에 나타내었다. 도 4 및 표 4, 표 5에 나타난 것과 같이 탄산염 0.4% 사용 시 우불고기의 관능적 품질이 유지되며, 이취/이미가 없고 고기 표면 과대연화로 인한 가루화 현상이 없지만, 전반적인 연화효과가 약하게 나타났다. 인산염 0.8%까지 사용 시 연화효과가 우수하지만, 용해성 문제로 산업 현장에서 사용이 불가하다. The results of measuring softening power by combining carbonate and phosphate are shown in Tables 4 and 4, and the sensory evaluation results are shown in Table 5. As shown in Figures 4, 4, and 5, when using 0.4% carbonate, the sensory quality of beef bulgogi is maintained, there is no odor/flavor, and there is no pulverization phenomenon due to over-softening of the meat surface, but the overall softening effect was weak. When using up to 0.8% phosphate, the softening effect is excellent, but it cannot be used in industrial fields due to solubility problems.

소재Material 사용량(% 고기대비)Consumption (% compared to meat) 경도결과(N/m2)Hardness result (N/m 2 ) 없음(대조군)None (control) -- 587,000587,000 탄산수소나트륨 sodium bicarbonate 0.40.4 526,446526,446 탄산수소나트륨 +인산염Sodium Bicarbonate + Phosphate 0.4/0.20.4/0.2 547,085547,085 0.4/0.40.4/0.4 451,586451,586 0.4/0.80.4/0.8 415,416415,416 탄산나트륨 sodium carbonate 0.40.4 500,412500,412 탄산나트륨 +인산염Sodium Carbonate + Phosphate 0.4/0.20.4/0.2 454,901454,901 0.4/0.40.4/0.4 468,388468,388 0.4/0.80.4/0.8 390,064390,064

실험군 experimental group 맛&향 taste & aroma 경도 Hardness 외관(색) Appearance (color) 대조군 control 불고기
고유의 맛
Bulgogi
unique taste
1 (질김)1 (tough) 불고기
고유의 색 (Brown)
Bulgogi
Unique color (Brown)
탄산나트륨 0.4% sodium carbonate 0.4% 1 (질김)1 (tough) 탄산나트륨 0.4% + 인산염 0.2% Sodium carbonate 0.4% + Phosphate 0.2% 3 (조금 부드러움)3 (slightly soft) 탄산나트륨 0.4% + 인산염 0.4% Sodium carbonate 0.4% + Phosphate 0.4% 3 (조금 부드러움)3 (slightly soft) 탄산나트륨 0.4% + 인산염 0.8% Sodium carbonate 0.4% + Phosphate 0.8% 4 (부드러움)4 (soft) 탄산수소나트륨 0.4% Sodium bicarbonate 0.4% 1 (질김)1 (tough) 탄산수소나트륨 0.4% + 인산염 0.2% Sodium bicarbonate 0.4% + Phosphate 0.2% 1 (질김)1 (tough) 탄산수소나트륨 0.4% + 인산염 0.4% Sodium bicarbonate 0.4% + Phosphate 0.4% 3 (조금 부드러움)3 (slightly soft) 탄산수소나트륨 0.4% + 인산염 0.8% Sodium Bicarbonate 0.4% + Phosphate 0.8% 4 (부드러움)4 (soft)

탄산염과 단백질 분해효소를 조합하여 연화력 측정한 결과를 표 6, 7 및 도 5, 6에 나타내었고, 관능평가 결과를 표 8에 나타내었다. 본 실험에서 사용한 효소는 국내에 유통되고 있는 식물성 단백질분해효소 파파인(Papain T100MG, Bision, Korea) 및 브로멜라인(Bromelain BR 1200, Indonesia)이다. The results of measuring softening power by combining carbonate and proteolytic enzymes are shown in Tables 6 and 7 and FIGS. 5 and 6, and the sensory evaluation results are shown in Table 8. The enzymes used in this experiment were papain (Papain T100MG, Bision, Korea) and bromelain (Bromelain BR 1200, Indonesia), which are distributed in Korea.

소재Material 사용량(% 고기대비)Consumption (% compared to meat) 경도결과(N/m2)Hardness result (N/m 2 ) 없음(대조군)None (control) -- 587,000587,000 브로멜라인bromelain 0.010.01 566,770566,770 0.0150.015 515,837515,837 탄산수소나트륨 sodium bicarbonate 0.40.4 527,583527,583 탄산수소나트륨 +브로멜라인Sodium Bicarbonate + Bromelain 0.4/0.010.4/0.01 439,805439,805 0.4/0.0150.4/0.015 487,992487,992 탄산나트륨 sodium carbonate 0.40.4 503,416503,416 탄산나트륨 +브로멜라인sodium carbonate + bromelain 0.4/0.010.4/0.01 465,492465,492 0.4/0.0150.4/0.015 399,403399,403

소재Material 사용량(% 고기대비)Consumption (% compared to meat) 경도결과(N/m2)Hardness result (N/m 2 ) 없음(대조군)None (control) -- 587,000587,000 파파인papain 0.010.01 541,483541,483 0.0150.015 579,026579,026 탄산수소나트륨 sodium bicarbonate 0.40.4 527,583527,583 탄산수소나트륨 +파파인Sodium Bicarbonate + Papain 0.4/0.010.4/0.01 553,852553,852 0.4/0.0150.4/0.015 570,256570,256 탄산나트륨 sodium carbonate 0.40.4 503,416503,416 탄산나트륨 +파파인Sodium Carbonate + Papain 0.4/0.010.4/0.01 466,740466,740 0.4/0.0150.4/0.015 429,663429,663

탄산염 0carbonate 0 탄산수소나트륨 0.4%Sodium bicarbonate 0.4% 탄산나트륨 0.4%sodium carbonate 0.4% 효소 0enzyme 0 1 (질김)1 (tough) 1 (질김)1 (tough) 1 (질김)1 (tough) 파파인 0.01%Papain 0.01% 1 (질김)1 (tough) 1 (질김)1 (tough) 4 (부드러운)4 (soft) 파파인 0.015%Papain 0.015% 1 (질김)1 (tough) 1 (질김)1 (tough) 5(매우 부드러운)5 (very soft) 브로멜라인 0.01%Bromelain 0.01% 1 (질김)1 (tough) 4 (부드러운)4 (soft) 4(부드러운)4 (soft) 브로멜라인 0.015%Bromelain 0.015% 3 (조금 부드러움)3 (slightly soft) 4 (부드러운)4 (soft) 5(매우 부드러운)5 (very soft)

표 6, 7 및 도 5, 6에 나타난 것과 같이 탄산염 및 효소 서로의 연화력 상승효과가 확인되었으며, 효소만 첨가한 경우에 나타나는 고기 표면의 과대 연화현상이 탄산염을 첨가함으로써 개선되는 것을 확인하였다. 또한 같은 농도 적용 시 탄산수소나트륨에 비하여 탄산나트륨이 효소와의 연화 시너지 효과가 더 현저한 것으로 나타났다. 또한, 효소는 같은 비율로 탄산나트륨과 조합 시, 효소 종류에 따라 연화력 차이가 없으나, 브로멜라인에 비하여 파파인 처리군의 관능적 품질이 더 우수하였다. As shown in Tables 6 and 7 and FIGS. 5 and 6, the synergistic effect of the softening power between the carbonate and the enzyme was confirmed, and it was confirmed that the over-softening of the meat surface, which appeared when only the enzyme was added, was improved by adding the carbonate. In addition, when the same concentration was applied, the softening synergistic effect of sodium carbonate with the enzyme was more remarkable than that of sodium bicarbonate. In addition, when the enzyme was combined with sodium carbonate in the same ratio, there was no difference in softening power depending on the type of enzyme, but the sensory quality of the papain-treated group was superior to that of bromelain.

탄산나트륨 0.4% 및 효소 0.015% 의 경우 이취/변색이 없지만 효소농도 증가에 따라 과대연화, 즉 고기 표준 가루화 현상이 다소 생기는 것을 확인하였으며, 연화효과 및 외관, 관능적 품질을 종합적으로 고려하였을 때 최적 비율 조합은 탄산나트륨 0.4% 및 파파인 0.01%인 것을 확인하였다. In the case of sodium carbonate 0.4% and enzyme 0.015%, there was no off-flavor/discoloration, but it was confirmed that over-softening, that is, standard meat powdering, occurred as the enzyme concentration increased. It was confirmed that the combination was 0.4% sodium carbonate and 0.01% papain.

추가 실험으로 알칼리염(탄산나트륨, 탄산수소나트륨 및 인산염)과 효소를 혼합하여 연화 효과 및 pH를 도 7, 표 9 및 표 10에 나타내었다. As an additional experiment, an alkali salt (sodium carbonate, sodium bicarbonate and phosphate) and an enzyme were mixed, and the softening effect and pH were shown in FIG. 7, Table 9, and Table 10.

도 7 및 표 9에 나타낸 것과 같이 알칼리염(탄산나트륨, 탄산수소나트륨 및 인산염)과 브로멜라인 같이 사용 시, 염 종류에 따라 경도 차이가 미미하였다. 알칼리염과 파파인 같이 사용 시, 탄산나트륨의 경도가 유의적으로 낮게 나왔다. 인산염 첨가 시 샘플 조리 후 인공적인 감칠맛이 강하게 나타나 관능적인 품질이 저하된 것으로 평가되었다. 표 10에 나타나는 것과 같이 소스와 탄산나트륨 및 단백질 분해효소를 포함하는 연화제 조성물을 혼합하였을 경우 pH 가 8.5 내지 8.8인 것을 확인하였다. As shown in FIGS. 7 and 9, when used together with alkali salts (sodium carbonate, sodium hydrogen carbonate and phosphate) and bromelain, the difference in hardness was insignificant depending on the salt type. When used together with alkali salt and papain, the hardness of sodium carbonate was significantly lower. It was evaluated that when phosphate was added, the sensory quality was deteriorated because the artificial umami taste was strong after the sample was cooked. As shown in Table 10, it was confirmed that when the source, the emollient composition including sodium carbonate and proteolytic enzyme were mixed, the pH was 8.5 to 8.8.

소재Material 사용량(% 고기대비)Consumption (% compared to meat) 경도결과(N/m2)Hardness result (N/m 2 ) 없음(대조군)None (control) -- 587,000587,000 탄산나트륨 +브로멜라인sodium carbonate + bromelain 0.4/0.010.4/0.01 465,492 465,492 탄산수소나트륨 +브로멜라인Sodium Bicarbonate + Bromelain 439,805 439,805 인산염 +브로멜라인Phosphate + Bromelain 455,067455,067 탄산나트륨 +파파인 Sodium Carbonate + Papain 466,740 466,740 탄산수소나트륨 + 파파인Sodium Bicarbonate + Papain 553,852 553,852 인산염 + 파파인Phosphate + Papain 504,338504,338

소스sauce 소스+
0.01B
Sauce+
0.01B
소스+ 0.01PSource + 0.01P 소스+
(탄산수소나트륨
0.4%+0.01B)
Sauce+
(Sodium Bicarbonate
0.4%+0.01B)
소스+
(탄산수소나트륨
0.4%+0.01P)
Sauce+
(Sodium Bicarbonate
0.4%+0.01P)
소스+
(탄산나트륨
0.4%+0.01B)
Sauce+
(Sodium Carbonate
0.4%+0.01B)
소스+
(탄산나트륨
0.4%+0.01P)
Sauce+
(Sodium Carbonate
0.4%+0.01P)
소스+
(인산염
0.4%+0.01B)
Sauce+
(phosphate
0.4%+0.01B)
소스+
(인산염
0.4%+0.01P)
Sauce+
(phosphate
0.4%+0.01P)
5.025.02 4.984.98 4.994.99 6.776.77 6.796.79 8.718.71 8.628.62 6.396.39 6.436.43

전술한 각 실시예에서 예시된 특징, 구조, 효과 등은 실시예들이 속하는 분야의 통상의 지식을 가지는 자에 의하여 다른 실시예들에 대해서도 조합 또는 변형되어 실시 가능하다. 따라서 이러한 조합과 변형에 관계된 내용들은 본 발명의 범위에 포함되는 것으로 해석되어야 할 것이다.Features, structures, effects, etc. exemplified in each of the above-described embodiments may be combined or modified for other embodiments by those of ordinary skill in the art to which the embodiments belong. Accordingly, the contents related to such combinations and modifications should be interpreted as being included in the scope of the present invention.

Claims (5)

알칼리염 96.0 내지 98.0 wt% 및 단백질 분해효소 2 내지 4 wt% 포함하는 것을 특징으로 하는 연화제 조성물.
An emollient composition comprising 96.0 to 98.0 wt% of an alkali salt and 2 to 4 wt% of a proteolytic enzyme.
제1항에 있어서,
상기 알칼리염은 탄산염 및 인산염 중 어느 1종 이상을 포함하고,
상기 탄산염은 탄산수소나트륨 및 탄산나트륨 중 어느 1종 이상을 포함하는 것을 특징으로 하는 연화제 조성물.
The method of claim 1,
The alkali salt includes any one or more of carbonate and phosphate;
The carbonate is an emollient composition comprising at least one of sodium hydrogen carbonate and sodium carbonate.
제1항에 있어서,
상기 단백질 분해효소는 파파인(Papain) 및 브로멜라인(Bromelain) 중 어느 1종 이상을 포함하는 것을 특징으로 하는 연화제 조성물.
The method of claim 1,
The proteolytic enzyme is an emollient composition comprising at least one of papain and bromelain.
연화제 조성물을 이용하여 원육을 연화시키는 연화육 제조방법으로서,
상기 연화제 조성물은 알칼리염 96.0 내지 98.0 wt% 및 단백질 분해효소 2 내지 4 wt% 포함하고,
원육 100 중량부에 대하여 상기 연화제 조성물을 0.1 내지 1 중량부 사용하는 것을 특징으로 하는 연화육 제조방법.
A method for preparing tenderized meat for softening raw meat using a softener composition, the method comprising:
The emollient composition comprises 96.0 to 98.0 wt% of an alkali salt and 2 to 4 wt% of a proteolytic enzyme,
A method for producing tenderized meat, characterized in that 0.1 to 1 part by weight of the softening agent composition is used based on 100 parts by weight of raw meat.
제4항에 있어서,
원육에 상기 연화제 조성물을 첨가하고 진공조건에서 텀블링하여 균질화한 후 진공 포장하여 20 내지 25 시간 동안 냉장 숙성시키는 것을 특징으로 하는 연화육 제조방법.
5. The method of claim 4,
A method for producing tenderized meat, characterized in that the softening composition is added to the raw meat, homogenized by tumbling in a vacuum condition, then vacuum-packed and refrigerated for 20 to 25 hours.
KR1020200159135A 2020-11-24 2020-11-24 Composition for softening meat and method for softening meat using the same KR20220071701A (en)

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